title: Investigating diet as the source of tetrodotoxin in Pleurobranchaea maculata

authors: Khor, Serena

abstract: The origin of tetrodotoxin (TTX) is highly debated; researchers have postulated either an endogenous or exogenous source with the host accumulating TTX symbiotically or via food chain transmission. The aim of this study was to determine whether the grey side-gilled sea slug (Pleurobranchaea maculata) could obtain TTX from a dietary source, and to attempt to identify this source through environmental surveys. Eighteen non-toxic P. maculata were maintained in aquariums and twelve were fed a TTX-containing diet. Three P. maculata were harvested after 1 h, 24 h, 17 days and 39 days and TTX concentrations in their stomach, gonad, mantle and remaining tissue/fluids determined using liquid chromatography-mass spectrometry. Tetrodotoxin was detected in all organs/tissue after 1 h with an average uptake of 32%. This decreased throughout the experiment (21%, 15% and 9%, respectively). Benthic surveys at sites with dense populations of toxic P. maculata detected very low or no TTX in other organisms. This study demonstrates that P. maculata can accumulate TTX through their diet. However, based on the absence of an identifiable TTX source in the environment, in concert with the extremely high TTX concentrations and short life spans of P. maculata, it is unlikely to be the sole TTX source for this species.

text: Investigating diet as the source of tetrodotoxin in Pleurobranchaea maculata The origin of tetrodotoxin (TTX) is highly debated; researchers have postulated either an endogenous or exogenous source with the host accumulating TTX symbiotically or via food chain transmission. The aim of this study was to determine whether the grey side-gilled sea slug (Pleurobranchaea maculata) could obtain TTX from a dietary source, and to attempt to identify this source through environmental surveys. Eighteen non-toxic P. maculata were maintained in aquariums and twelve were fed a TTX-containing diet. Three P. maculata were harvested after 1 h, 24 h, 17 days and 39 days and TTX concentrations in their stomach, gonad, mantle and remaining tissue/fluids determined using liquid chromatography-mass spectrometry. Tetrodotoxin was detected in all organs/tissue after 1 h with an average uptake of 32%. This decreased throughout the experiment (21%, 15% and 9%, respectively). Benthic surveys at sites with dense populations of toxic P. maculata detected very low or no TTX in other organisms. This study demonstrates that P. maculata can accumulate TTX through their diet. However, based on the absence of an identifiable TTX source in the environment, in concert with the extremely high TTX concentrations and short life spans of P. maculata, it is unlikely to be the sole TTX source for this species.

year: 2014-01

title: The third-order law for magnetohydrodynamic turbulence with shear: Numerical investigation

authors: Wan, Minping

subjects: numerical analysis

abstract: The scaling laws of third-order structure functions for isotropic, homogeneous, and incompressible magnetohydrodynamic (MHD) turbulence relate the observable structure function with the energy dissipation rate. Recently [ Wan et al. Phys. Plasmas 16, 090703 (2009) ], the theory was extended to the case in which a constant velocity shear is present, motivated by the application of the third-order law to the solar wind. We use direct numerical simulations of two-dimensional MHD with shear to confirm this new generalization of the theory. The presence of the shear effect broadens the circumstances in which the law can be applied. Important implications for laboratory and space plasmas are discussed.

text: The third-order law for magnetohydrodynamic turbulence with shear: Numerical investigation The scaling laws of third-order structure functions for isotropic, homogeneous, and incompressible magnetohydrodynamic (MHD) turbulence relate the observable structure function with the energy dissipation rate. Recently [ Wan et al. Phys. Plasmas 16, 090703 (2009) ], the theory was extended to the case in which a constant velocity shear is present, motivated by the application of the third-order law to the solar wind. We use direct numerical simulations of two-dimensional MHD with shear to confirm this new generalization of the theory. The presence of the shear effect broadens the circumstances in which the law can be applied. Important implications for laboratory and space plasmas are discussed.

year: 2010

title: The city of Rotorua and its meaning to Ngati Whakaue

authors: Te Awekotuku, Ngahuia

subjects: New Zealand

abstract: This paper discusses the history and settlement of the city of Rotorua as it relates to Ngati Whakaue.

text: The city of Rotorua and its meaning to Ngati Whakaue This paper discusses the history and settlement of the city of Rotorua as it relates to Ngati Whakaue.

year: 1976-08