title: Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract

authors: Badiali, Luca

abstract: Background: G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target. The Adhesion subfamily is the second largest one of GPCRs and its several members are known to mediate neural development and immune system functioning through cell-cell and cell-matrix interactions. The distribution of these receptors has not been characterized in detail in the gastrointestinal (GI) tract. Here we present the first comprehensive anatomical profiling of mRNA expression of all 30 Adhesion GPCRs in the rat GI tract divided into twelve subsegments. Methods: Using RT-qPCR, we studied the expression of Adhesion GPCRs in the esophagus, the corpus and antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum. Results: We found that twenty-one Adhesion GPCRs (70%) had a widespread (expressed in five or more segments) or ubiquitous (expressed in eleven or more segments) distribution, seven (23%) were restricted to a few segments of the GI tract and two were not expressed in any segment. Most notably, almost all Group III members were ubiquitously expressed, while the restricted expression was characteristic for the majority of group VII members, hinting at more specific/localized roles for some of these receptors. Conclusions: Overall, the distribution of Adhesion GPCRs points to their important role in GI tract functioning and defines them as a potentially crucial target for pharmacological interventions. © 2012 Badiali et al.; licensee BioMed Central Ltd.

text: Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract Background: G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target. The Adhesion subfamily is the second largest one of GPCRs and its several members are known to mediate neural development and immune system functioning through cell-cell and cell-matrix interactions. The distribution of these receptors has not been characterized in detail in the gastrointestinal (GI) tract. Here we present the first comprehensive anatomical profiling of mRNA expression of all 30 Adhesion GPCRs in the rat GI tract divided into twelve subsegments. Methods: Using RT-qPCR, we studied the expression of Adhesion GPCRs in the esophagus, the corpus and antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum. Results: We found that twenty-one Adhesion GPCRs (70%) had a widespread (expressed in five or more segments) or ubiquitous (expressed in eleven or more segments) distribution, seven (23%) were restricted to a few segments of the GI tract and two were not expressed in any segment. Most notably, almost all Group III members were ubiquitously expressed, while the restricted expression was characteristic for the majority of group VII members, hinting at more specific/localized roles for some of these receptors. Conclusions: Overall, the distribution of Adhesion GPCRs points to their important role in GI tract functioning and defines them as a potentially crucial target for pharmacological interventions. © 2012 Badiali et al.; licensee BioMed Central Ltd.

year: 2012

title: Evolution of the global carbon cycle and climate regulation on Earth

authors: Isson, Terry T.

subjects: Carbon cycle

abstract: The existence of stabilizing feedbacks within Earth's climate system is generally thought to be necessary for the persistence of liquid water and life. Over the course of Earth's history, Earth's atmospheric composition appears to have adjusted to the gradual increase in solar luminosity, resulting in persistently habitable surface temperatures. With limited exceptions, the Earth system has been observed to recover rapidly from pulsed climatic perturbations. Carbon dioxide (CO₂) regulation via negative feedbacks within the coupled global carbon‐silica cycles are classically viewed as the main processes giving rise to climate stability on Earth. Here we review the long‐term global carbon cycle budget, and how the processes modulating Earth's climate system have evolved over time. Specifically, we focus on the relative roles that shifts in carbon sources and sinks have played in driving long‐term changes in atmospheric pCO₂. We make the case that marine processes are an important component of the canonical silicate weathering feedback, and have played a much more important role in 𝘱CO₂ regulation than traditionally imagined. Notably, geochemical evidence indicate that the weathering of marine sediments and off‐axis basalt alteration act as major carbon sinks. However, this sink was potentially dampened during Earth's early history when oceans had higher levels of dissolved silicon (Si), iron (Fe), and magnesium (Mg), and instead likely fostered more extensive carbon recycling within the ocean‐atmosphere system via reverse weathering—that in turn acted to elevate ocean‐atmosphere CO₂ levels.

text: Evolution of the global carbon cycle and climate regulation on Earth The existence of stabilizing feedbacks within Earth's climate system is generally thought to be necessary for the persistence of liquid water and life. Over the course of Earth's history, Earth's atmospheric composition appears to have adjusted to the gradual increase in solar luminosity, resulting in persistently habitable surface temperatures. With limited exceptions, the Earth system has been observed to recover rapidly from pulsed climatic perturbations. Carbon dioxide (CO₂) regulation via negative feedbacks within the coupled global carbon‐silica cycles are classically viewed as the main processes giving rise to climate stability on Earth. Here we review the long‐term global carbon cycle budget, and how the processes modulating Earth's climate system have evolved over time. Specifically, we focus on the relative roles that shifts in carbon sources and sinks have played in driving long‐term changes in atmospheric pCO₂. We make the case that marine processes are an important component of the canonical silicate weathering feedback, and have played a much more important role in 𝘱CO₂ regulation than traditionally imagined. Notably, geochemical evidence indicate that the weathering of marine sediments and off‐axis basalt alteration act as major carbon sinks. However, this sink was potentially dampened during Earth's early history when oceans had higher levels of dissolved silicon (Si), iron (Fe), and magnesium (Mg), and instead likely fostered more extensive carbon recycling within the ocean‐atmosphere system via reverse weathering—that in turn acted to elevate ocean‐atmosphere CO₂ levels.

year: 2020

title: ‘Let’s Get Wild’: Sensuous Geographies of Kāwhia Kai and Hokitika Wildfoods Festivals in Aōtearoa New Zealand

authors: Modlik, Melanie

subjects: Food Festivals

abstract: Food Festivals allow communities to celebrate local food and drink. This original thesis examines the mutual relationship between food festival organisers, festival attendees and festival spaces. Two unique New Zealand regional food festivals – Kāwhia Kai on the West Coast of the North Island and Wildfoods Hokitika on the West Coast of the South Island - are critically examined. The research addresses three questions. First, how do these food festivals construct their locations and vice versa? Second, in what ways do food festivals contribute to place identity? And, third, how do food festival attendees sensually experience the festivals?
The concept of embodiment provides the overarching framework for this research. With academic interests in both tourism and geography, I weave understandings of culinary tourism, festivals, tourist embodied experiences, sensuality, visercality and abjection to address my research questions. I use this theoretical ‘toolbox’ alongside qualitative methodological approaches which enable research participants to express their embodied experiences and emotions. Ten semi-structured interviews were conducted with festival organisers and food stall holders. Participant ‘sensing’ was carried out in both Kāwhia and Hokitika. In thinking about the social, cultural and ‘felt’ aspects of food, I also use my own body as an instrument of research. Promotional texts were collected and all data analysed using critical discourse analysis.
The empirical material is divided into two themes. The first theme focuses on location and place identity. Concepts of ‘place attachment’ and ‘sense of belonging’ construct food festival regions. The formation of ‘new colonial’ and ‘new Māori’ traditions is another way in which these food festivals created unique place identities. The second theme highlights the embodied experiences and spaces of both food festivals. Bodily senses, place and spaces are created through visceral and abject reactions to food experiences.
Food festivals have the potential to enhance the economic, social and cultural wellbeing of regional communities. The success of food festivals, however, is dependent on multi-sensual embodied experiences.

text: ‘Let’s Get Wild’: Sensuous Geographies of Kāwhia Kai and Hokitika Wildfoods Festivals in Aōtearoa New Zealand Food Festivals allow communities to celebrate local food and drink. This original thesis examines the mutual relationship between food festival organisers, festival attendees and festival spaces. Two unique New Zealand regional food festivals – Kāwhia Kai on the West Coast of the North Island and Wildfoods Hokitika on the West Coast of the South Island - are critically examined. The research addresses three questions. First, how do these food festivals construct their locations and vice versa? Second, in what ways do food festivals contribute to place identity? And, third, how do food festival attendees sensually experience the festivals?
The concept of embodiment provides the overarching framework for this research. With academic interests in both tourism and geography, I weave understandings of culinary tourism, festivals, tourist embodied experiences, sensuality, visercality and abjection to address my research questions. I use this theoretical ‘toolbox’ alongside qualitative methodological approaches which enable research participants to express their embodied experiences and emotions. Ten semi-structured interviews were conducted with festival organisers and food stall holders. Participant ‘sensing’ was carried out in both Kāwhia and Hokitika. In thinking about the social, cultural and ‘felt’ aspects of food, I also use my own body as an instrument of research. Promotional texts were collected and all data analysed using critical discourse analysis.
The empirical material is divided into two themes. The first theme focuses on location and place identity. Concepts of ‘place attachment’ and ‘sense of belonging’ construct food festival regions. The formation of ‘new colonial’ and ‘new Māori’ traditions is another way in which these food festivals created unique place identities. The second theme highlights the embodied experiences and spaces of both food festivals. Bodily senses, place and spaces are created through visceral and abject reactions to food experiences.
Food festivals have the potential to enhance the economic, social and cultural wellbeing of regional communities. The success of food festivals, however, is dependent on multi-sensual embodied experiences.

year: 2015