title: Developing an eDNA approach for wetland biomonitoring: Insights on technical and conventional approaches

authors: Bird, Starsha

abstract: Wetlands are ecologically and culturally significant ecosystems that are experiencing biodiversity declines globally. Biomonitoring techniques that use environmental DNA (eDNA) to detect and monitor biodiversity are well established in lake, riverine, and marine ecosystems. However, their use in wetlands requires further development due to the presence of sediments that block eDNA filters to limit water filtration, alongside a lack of standardized methodology. In this study, we examined eDNA dynamics to understand spatiotemporal biodiversity patterns in an Aotearoa New Zealand wetland and to optimize their application to wetland-specific challenges. We sampled four sites across Opuatia Wetland at three time points during an austral spring. We conducted conventional taxonomic surveys, tested three different filter sizes (1.2 μm, 5 μm, and semi-quantitative dacron filters), and assessed our ability to detect foreign DNA (from kea; Nestor notabilis) at different time points and distances post-release. We found significant differences in DNA sequence composition across time and space, and when using different sized filters. eDNA data generally complemented (versus replaced) conventional survey and identification methods, with certain species only detected by one method or the other. Taxonomic resolution of conventional sampling and identification methods often exceeded that of eDNA. Foreign DNA was detectable 10 m from its release point for up to 1 week post-release. Our results provide new considerations for future eDNA research in wetland environments, where rapid biomonitoring techniques are needed to support conservation and preservation.

text: Developing an eDNA approach for wetland biomonitoring: Insights on technical and conventional approaches Wetlands are ecologically and culturally significant ecosystems that are experiencing biodiversity declines globally. Biomonitoring techniques that use environmental DNA (eDNA) to detect and monitor biodiversity are well established in lake, riverine, and marine ecosystems. However, their use in wetlands requires further development due to the presence of sediments that block eDNA filters to limit water filtration, alongside a lack of standardized methodology. In this study, we examined eDNA dynamics to understand spatiotemporal biodiversity patterns in an Aotearoa New Zealand wetland and to optimize their application to wetland-specific challenges. We sampled four sites across Opuatia Wetland at three time points during an austral spring. We conducted conventional taxonomic surveys, tested three different filter sizes (1.2 μm, 5 μm, and semi-quantitative dacron filters), and assessed our ability to detect foreign DNA (from kea; Nestor notabilis) at different time points and distances post-release. We found significant differences in DNA sequence composition across time and space, and when using different sized filters. eDNA data generally complemented (versus replaced) conventional survey and identification methods, with certain species only detected by one method or the other. Taxonomic resolution of conventional sampling and identification methods often exceeded that of eDNA. Foreign DNA was detectable 10 m from its release point for up to 1 week post-release. Our results provide new considerations for future eDNA research in wetland environments, where rapid biomonitoring techniques are needed to support conservation and preservation.

year: 2024

title: Chinese clientele at Chinese hotels—Preferences and satisfaction

authors: Gu, Huimin

subjects: Chinese hotels

abstract: The paper examines the proposal that levels of satisfaction with mainland Chinese hotels by the domestic market are related to performance in core services to which clients attribute high levels of importance. Regression analysis indicates that the main determinants of satisfaction are the external environment, reputation and cleanliness of the bedroom. It is suggested that key components of the hotel product such as a comfortable bed are akin to hygiene factors in Herzberg's theory of human relations, that is, their absence causes dissatisfaction, but their presence is insufficient to generate high levels of satisfaction. The sample comprises 941 Chinese respondents.

text: Chinese clientele at Chinese hotels—Preferences and satisfaction The paper examines the proposal that levels of satisfaction with mainland Chinese hotels by the domestic market are related to performance in core services to which clients attribute high levels of importance. Regression analysis indicates that the main determinants of satisfaction are the external environment, reputation and cleanliness of the bedroom. It is suggested that key components of the hotel product such as a comfortable bed are akin to hygiene factors in Herzberg's theory of human relations, that is, their absence causes dissatisfaction, but their presence is insufficient to generate high levels of satisfaction. The sample comprises 941 Chinese respondents.

year: 2008

title: Te Ahi Kā

authors: Temara, Pou

subjects: Ahi kā

abstract: Ko te urutaumatua o tēnei tuhinga, ko te matapaki i te ahi kā. He aha tēnei tikanga? I pēhea i ara ake ai? He aha ōna mātāpono, ōna taipitopito? Koia te tūāpapa mō te tuhinga nei. Tuarua, ko te wānanga i te tikanga Māori me ōna rerenga pērā i te whakapapa. Ko te pātai ka puta i tēnei wāhanga o te wānanga ko tēnei nā, he aha te wāhi o te whakapapa ki te ahi kā? Ko te ngako o tēnei tuhinga ko te wetewete i te tikanga ahi kā kia whai tikanga ai te whakataukī nei, ‘ko te ahi kā te mana o te hapū ki te whenua’.

text: Te Ahi Kā Ko te urutaumatua o tēnei tuhinga, ko te matapaki i te ahi kā. He aha tēnei tikanga? I pēhea i ara ake ai? He aha ōna mātāpono, ōna taipitopito? Koia te tūāpapa mō te tuhinga nei. Tuarua, ko te wānanga i te tikanga Māori me ōna rerenga pērā i te whakapapa. Ko te pātai ka puta i tēnei wāhanga o te wānanga ko tēnei nā, he aha te wāhi o te whakapapa ki te ahi kā? Ko te ngako o tēnei tuhinga ko te wetewete i te tikanga ahi kā kia whai tikanga ai te whakataukī nei, ‘ko te ahi kā te mana o te hapū ki te whenua’.

year: 2012-05