title: Fish biosurveillance by genetic methods: a feasibility study

authors: Knox, Matthew Andrew

subjects: New Zealand

abstract: We evaluated the possibility of isolating DNA from environmental samples to detect and identify invasive fish species based on variation in the sequences for mitochondrial gene region. Goldfish CO1 DNA was successfully amplified from the water in a tank containing goldfish. Sequencing results verified that the DNA extracted from water matched goldfish sequences obtained from DNA extracted from tissue from other sources.
We also evaluated the potential of a DNA-based system to detect and identify invasive fish species based on difference in the length of several gene regions. Sequences for the 12S and 16S rRNA genes, cytochrome c oxidase subunit 1 (CO1) gene and the d-loop region downloaded from GenBank suggested that sequences from these genes would contain enough variation to distinguish six pest fish species (koi carp, rudd, brown bullhead catfish, goldfish, perch and gambusia) introduced to New Zealand freshwaters. A 250-nucleotide region of the 16S rRNA gene was successfully amplified and sequenced from fish tissue, faecal material and fish mucus. While sequences from these regions could distinguish fish species, there was insufficient variation in length to identify each fish species. Future directions for this research are suggested.

text: Fish biosurveillance by genetic methods: a feasibility study We evaluated the possibility of isolating DNA from environmental samples to detect and identify invasive fish species based on variation in the sequences for mitochondrial gene region. Goldfish CO1 DNA was successfully amplified from the water in a tank containing goldfish. Sequencing results verified that the DNA extracted from water matched goldfish sequences obtained from DNA extracted from tissue from other sources.
We also evaluated the potential of a DNA-based system to detect and identify invasive fish species based on difference in the length of several gene regions. Sequences for the 12S and 16S rRNA genes, cytochrome c oxidase subunit 1 (CO1) gene and the d-loop region downloaded from GenBank suggested that sequences from these genes would contain enough variation to distinguish six pest fish species (koi carp, rudd, brown bullhead catfish, goldfish, perch and gambusia) introduced to New Zealand freshwaters. A 250-nucleotide region of the 16S rRNA gene was successfully amplified and sequenced from fish tissue, faecal material and fish mucus. While sequences from these regions could distinguish fish species, there was insufficient variation in length to identify each fish species. Future directions for this research are suggested.

year: 2008

title: Solar wind turbulent heating by interstellar pickup protons: 2-component model

authors: Isenberg, Philip A.

subjects: interstellar pickup ions

abstract: We apply a recently developed 2-component phenomenology to the turbulent heating of the core solar wind protons as seen at the Voyager 2 spacecraft. We find that this new description improves the model predictions of core temperature and correlation scale of the fluctuations, yielding excellent agreement with the Voyager measurements. However, the model fluctuation intensity substantially exceeds the Voyager measurements in the outer heliosphere, indicating that this picture needs further refinement.

text: Solar wind turbulent heating by interstellar pickup protons: 2-component model We apply a recently developed 2-component phenomenology to the turbulent heating of the core solar wind protons as seen at the Voyager 2 spacecraft. We find that this new description improves the model predictions of core temperature and correlation scale of the fluctuations, yielding excellent agreement with the Voyager measurements. However, the model fluctuation intensity substantially exceeds the Voyager measurements in the outer heliosphere, indicating that this picture needs further refinement.

year: 2010

title: Supervising research in Māori cultural contexts: a decolonizing, relational response

authors: Berryman, Mere

abstract: We have collaborated for 25 years as indigenous Māori and non- Māori researchers undertaking research with Māori families, their schools and communities. We have endeavored to meet our responsibilities to the Māori people (indigenous inhabitants of New Zealand) and communities with whom we have researched, as well as meet the requirements and responsibilities of our academic institutions. In this paper, we reflect on the implications of these responsibilities for our work as supervisors of master’s and doctoral students (Māori and non-Māori) who seek to draw on decolonizing methodologies as they undertake research in Māori cultural contexts. We draw on the experiences and interactions we have had with four different postgraduate students whose research on improving educational outcomes for Māori students has required them to engage and participate in Māori cultural contexts.

text: Supervising research in Māori cultural contexts: a decolonizing, relational response We have collaborated for 25 years as indigenous Māori and non- Māori researchers undertaking research with Māori families, their schools and communities. We have endeavored to meet our responsibilities to the Māori people (indigenous inhabitants of New Zealand) and communities with whom we have researched, as well as meet the requirements and responsibilities of our academic institutions. In this paper, we reflect on the implications of these responsibilities for our work as supervisors of master’s and doctoral students (Māori and non-Māori) who seek to draw on decolonizing methodologies as they undertake research in Māori cultural contexts. We draw on the experiences and interactions we have had with four different postgraduate students whose research on improving educational outcomes for Māori students has required them to engage and participate in Māori cultural contexts.

year: 2017