title: A Generic Alerting Service for Digital Libraries

authors: Buchanan, George

subjects: alerting

abstract: Users of modern digital libraries (DLs) can keep themselves up-to-date by searching and browsing their favorite collections, or more conveniently by resorting to an alerting service. The alerting service notifies its clients about new or changed documents. Proprietary and mediating alerting services fail to fluidly integrate information from differing collections. This paper analyses the conceptual requirements of this much-sought after service for digital libraries. We demonstrate that the differing concepts of digital libraries and its underlying technical design has extensive influence (a) the expectations, needs and interests of users regarding an alerting service, and (b) on the technical possibilities of the implementation of the service. Our findings will show that the range of issues surrounding alerting services for digital libraries, their design and use is greater than one may anticipate. We also show that, conversely, the requirements for an alerting service have considerable impact on the concepts of DL design. Our findings should be of interest for librarians as well as system designers. We highlight and discuss the far-reaching implications for the design of, and interaction with, libraries. This paper discusses the lessons learned from building such a distributed alerting service. We present our prototype implementation as a proof-of-concept for an alerting service for open DL software.

text: A Generic Alerting Service for Digital Libraries Users of modern digital libraries (DLs) can keep themselves up-to-date by searching and browsing their favorite collections, or more conveniently by resorting to an alerting service. The alerting service notifies its clients about new or changed documents. Proprietary and mediating alerting services fail to fluidly integrate information from differing collections. This paper analyses the conceptual requirements of this much-sought after service for digital libraries. We demonstrate that the differing concepts of digital libraries and its underlying technical design has extensive influence (a) the expectations, needs and interests of users regarding an alerting service, and (b) on the technical possibilities of the implementation of the service. Our findings will show that the range of issues surrounding alerting services for digital libraries, their design and use is greater than one may anticipate. We also show that, conversely, the requirements for an alerting service have considerable impact on the concepts of DL design. Our findings should be of interest for librarians as well as system designers. We highlight and discuss the far-reaching implications for the design of, and interaction with, libraries. This paper discusses the lessons learned from building such a distributed alerting service. We present our prototype implementation as a proof-of-concept for an alerting service for open DL software.

year: 2005-06-07

title: An investigation of the accelerated loss of diastase (α-amylase) activity in mānuka honey

authors: Kaur, Harmanpreet

abstract: New Zealand’s multi-million dollar apiculture industry exports over 10,000 tonnes of honey annually. Each country has import guidelines to ensure honey quality. The diastase activity assay is used to determine whether honey has been exposed to harsh conditions (excess storage time or high temperatures will denature diastase) and reports the activity as diastase number (DN), where DN must be >8 for exports. Unfortunately, mānuka honey (NZ’s high value honey with unique properties) fails the diastase assay more often than other NZ floral honeys. Bioactive compounds [methylglyoxal (MGO), dihydroxyacetone (DHA) and 3-phenyllactic acid (3PLA)] in mānuka honey were predicted to decrease diastase activity by forming modifications on diastase. Hence, the extent, diversity, location and functional impacts of these modifications on diastase were investigated.

Two fresh mānuka honeys, a clover control and clover spiked (MGO, DHA, 3PLA, 3PLA+MGO and 3PLA+DHA) honey samples were stored at 27 °C for ~200 days and periodically tested to observe chemical compositional changes in relation to the loss of diastase activity. The results corroborated previous data; DN decreased in honey over time with increased loss rates observed in mānuka. In the spiked clover samples both DHA and MGO increased rates of diastase activity loss and these rates were enhanced by 3PLA (a proton donor). Enzyme kinetics (enzyme-substrate binding and enzyme rate constants) suggest that denaturation is not the sole mechanism for reduced DN values in honey over time, rather, surface level modifications from bioactive compounds are affecting the affinity of diastase for the substrate.

Diastase was isolated from the honeys (days 0 and ~200) using SDS-PAGE gels and analysed with LC-MS/MS to identify surface modifications on diastase. Results showed evidence of diastase modification over the time trial for all honeys. This occurred in unique locations for both mānuka honeys and MGO and 3PLA+DHA spiked clover honeys. Some of these locations were in proximity to the active site of diastase.

The most abundant honey protein, MRJP1, was used to accurately identify trends in modification changes on proteins over time as a model for the effect occurring on diastase (since similar reactive amino acids are present on both MRJP1 and diastase). All honeys (except the clover control) had an increased number of modifications. The largest increases were observed for the MGO, DHA and 3PLA+MGO spiked honeys. Fewer modifications were detected for the 3PLA+DHA spiked honey despite this having the most pronounced changes in the enzyme kinetic parameters. This is likely because the modification library focused on MGO-induced modifications and did not account for DHA modifications.

In summary, MGO modifications on proteins in honey were observed. DHA modifications and the role of 3PLA as a proton donor were deduced from the enzyme kinetic parameters. While time and temperature denature diastase, surface modifications on diastase may be contributing to the accelerated loss of diastase activity observed for mānuka honey. This necessitates the reassessment of diastase activity as a test for honey quality, especially in the context of complex honey matrices (such as mānuka honey).

text: An investigation of the accelerated loss of diastase (α-amylase) activity in mānuka honey New Zealand’s multi-million dollar apiculture industry exports over 10,000 tonnes of honey annually. Each country has import guidelines to ensure honey quality. The diastase activity assay is used to determine whether honey has been exposed to harsh conditions (excess storage time or high temperatures will denature diastase) and reports the activity as diastase number (DN), where DN must be >8 for exports. Unfortunately, mānuka honey (NZ’s high value honey with unique properties) fails the diastase assay more often than other NZ floral honeys. Bioactive compounds [methylglyoxal (MGO), dihydroxyacetone (DHA) and 3-phenyllactic acid (3PLA)] in mānuka honey were predicted to decrease diastase activity by forming modifications on diastase. Hence, the extent, diversity, location and functional impacts of these modifications on diastase were investigated.

Two fresh mānuka honeys, a clover control and clover spiked (MGO, DHA, 3PLA, 3PLA+MGO and 3PLA+DHA) honey samples were stored at 27 °C for ~200 days and periodically tested to observe chemical compositional changes in relation to the loss of diastase activity. The results corroborated previous data; DN decreased in honey over time with increased loss rates observed in mānuka. In the spiked clover samples both DHA and MGO increased rates of diastase activity loss and these rates were enhanced by 3PLA (a proton donor). Enzyme kinetics (enzyme-substrate binding and enzyme rate constants) suggest that denaturation is not the sole mechanism for reduced DN values in honey over time, rather, surface level modifications from bioactive compounds are affecting the affinity of diastase for the substrate.

Diastase was isolated from the honeys (days 0 and ~200) using SDS-PAGE gels and analysed with LC-MS/MS to identify surface modifications on diastase. Results showed evidence of diastase modification over the time trial for all honeys. This occurred in unique locations for both mānuka honeys and MGO and 3PLA+DHA spiked clover honeys. Some of these locations were in proximity to the active site of diastase.

The most abundant honey protein, MRJP1, was used to accurately identify trends in modification changes on proteins over time as a model for the effect occurring on diastase (since similar reactive amino acids are present on both MRJP1 and diastase). All honeys (except the clover control) had an increased number of modifications. The largest increases were observed for the MGO, DHA and 3PLA+MGO spiked honeys. Fewer modifications were detected for the 3PLA+DHA spiked honey despite this having the most pronounced changes in the enzyme kinetic parameters. This is likely because the modification library focused on MGO-induced modifications and did not account for DHA modifications.

In summary, MGO modifications on proteins in honey were observed. DHA modifications and the role of 3PLA as a proton donor were deduced from the enzyme kinetic parameters. While time and temperature denature diastase, surface modifications on diastase may be contributing to the accelerated loss of diastase activity observed for mānuka honey. This necessitates the reassessment of diastase activity as a test for honey quality, especially in the context of complex honey matrices (such as mānuka honey).

year: 2025

title: An evaluation of Te Rau Puawai workforce 100: Stakeholder perspectives

authors: Nikora, Linda Waimarie

subjects: Maori students

abstract: To evaluate the Te Rau Puawai programme, the Ministry of Health commissioned the
Maori and Psychology Research Unit of the University of Waikato in July 2001. The
overall aim of the evaluation was to provide the Ministry with a clearer understanding
of the programme including: the perceived critical success factors, the barriers if any
regarding Te Rau Puawai, the impact of the programme, the extent to which the
programme may be transferable, gaps in the programme, and suggested
improvements.
There are a number of stakeholders who do not have a direct role in the provision of
Te Rau Puawai. These people are not involved in the day to day running of Te Rau
Puawai (as do, for example, the coordinator, support team or academic mentors),
nevertheless they play an important role, contributing in a variety of ways to the
programme.

text: An evaluation of Te Rau Puawai workforce 100: Stakeholder perspectives To evaluate the Te Rau Puawai programme, the Ministry of Health commissioned the
Maori and Psychology Research Unit of the University of Waikato in July 2001. The
overall aim of the evaluation was to provide the Ministry with a clearer understanding
of the programme including: the perceived critical success factors, the barriers if any
regarding Te Rau Puawai, the impact of the programme, the extent to which the
programme may be transferable, gaps in the programme, and suggested
improvements.
There are a number of stakeholders who do not have a direct role in the provision of
Te Rau Puawai. These people are not involved in the day to day running of Te Rau
Puawai (as do, for example, the coordinator, support team or academic mentors),
nevertheless they play an important role, contributing in a variety of ways to the
programme.

year: 2002-05-01