title: Modelling single cell electroporation with bipolar pulse: Simulating dependance of electroporated fractional pore area on the bipolar field frequency

authors: Talele, Sadhana

subjects: electroporation

abstract: Electroporation EP, in which external electric field pulses create transient pores in a cell membrane, is an important technique for delivery of DNA and drugs into the cell. To enable entry of DNA into cells, the pores should have sufficiently large radii, remain open long enough for the DNA chain to enter the cell, and should not cause membrane rupture. A numerical model for a single spherical cell electroporated by application of direct and/or alternating external electric field pulses has been developed. The model is used to calculate the transmembrane potential, the number of pores and the the fraction of area occupied by the pores (fractional pore area FPA) in response to the various electric field pulses. Presented here are simulation results used to compare the extent of electroporation (fractional pore area FPA) in response to eletric field pulses of different frequencies in a range of extracellular conductivity for two cell raii. It is also observed that a 1 MHz bipolar sinusoidal applied electric field pulse reduces the relative difference in fractional pore area between the two cell sizes compared to a 100 kHz pulse.

text: Modelling single cell electroporation with bipolar pulse: Simulating dependance of electroporated fractional pore area on the bipolar field frequency Electroporation EP, in which external electric field pulses create transient pores in a cell membrane, is an important technique for delivery of DNA and drugs into the cell. To enable entry of DNA into cells, the pores should have sufficiently large radii, remain open long enough for the DNA chain to enter the cell, and should not cause membrane rupture. A numerical model for a single spherical cell electroporated by application of direct and/or alternating external electric field pulses has been developed. The model is used to calculate the transmembrane potential, the number of pores and the the fraction of area occupied by the pores (fractional pore area FPA) in response to the various electric field pulses. Presented here are simulation results used to compare the extent of electroporation (fractional pore area FPA) in response to eletric field pulses of different frequencies in a range of extracellular conductivity for two cell raii. It is also observed that a 1 MHz bipolar sinusoidal applied electric field pulse reduces the relative difference in fractional pore area between the two cell sizes compared to a 100 kHz pulse.

year: 2010

title: Enacted stigma experiences and protective factors are strongly associated with mental health outcomes of transgender people in Aotearoa/New Zealand

authors: Tan, Kyle K. H.

abstract: Introduction: International evidence has found large mental health inequities among transgender people and demonstrates that mental health outcomes are associated with enacted stigma experiences and protective factors. This study aimed to examine the extent of associations of enacted stigma experiences specific to transgender people alongside protective factors with mental health of transgender people in Aotearoa/New Zealand. 
Methods: The 2018 Counting Ourselves survey was a nationwide community-based study of transgender people (N = 1178, Mage = 29.5) living in Aotearoa/New Zealand. The survey assessed a wide range of gender minority stress experiences and protective factors that comprised primary (support from friends and family) and secondary social ties (neighborhood and transgender community belongingness). We calculated the predicted probabilities that transgender people exhibit very high psychological distress level, non-suicidal self-injury, and suicidal risks with different combinations and exposure profiles of enacted stigma and protective factors. 
Results: Our findings demonstrated that enacted stigma was associated with negative mental health, and support of friends and family was linked to better outcomes across all mental health measures. Beyond primary social ties, sense of belongingness to neighborhood and transgender communities were linked to reduced odds of psychological distress and suicidal ideation. For those scoring high on enacted stigma and low on protective factors, our model revealed a 25% probability of attempting suicide in the last year compared to 3% for those scoring low on enacted stigma and high on protective factors. 
Conclusions: Echoing previous findings, this study demonstrates that transgender people across Aotearoa/New Zealand are less likely to manifest life-threatening mental health outcomes if they experience low levels of enacted stigma and high levels of access to protective factors. Our findings suggest a need to address the enacted stigma that transgender people face across interpersonal and structural settings, and also to enhance social supports that are gender affirmative for this population.

text: Enacted stigma experiences and protective factors are strongly associated with mental health outcomes of transgender people in Aotearoa/New Zealand Introduction: International evidence has found large mental health inequities among transgender people and demonstrates that mental health outcomes are associated with enacted stigma experiences and protective factors. This study aimed to examine the extent of associations of enacted stigma experiences specific to transgender people alongside protective factors with mental health of transgender people in Aotearoa/New Zealand. 
Methods: The 2018 Counting Ourselves survey was a nationwide community-based study of transgender people (N = 1178, Mage = 29.5) living in Aotearoa/New Zealand. The survey assessed a wide range of gender minority stress experiences and protective factors that comprised primary (support from friends and family) and secondary social ties (neighborhood and transgender community belongingness). We calculated the predicted probabilities that transgender people exhibit very high psychological distress level, non-suicidal self-injury, and suicidal risks with different combinations and exposure profiles of enacted stigma and protective factors. 
Results: Our findings demonstrated that enacted stigma was associated with negative mental health, and support of friends and family was linked to better outcomes across all mental health measures. Beyond primary social ties, sense of belongingness to neighborhood and transgender communities were linked to reduced odds of psychological distress and suicidal ideation. For those scoring high on enacted stigma and low on protective factors, our model revealed a 25% probability of attempting suicide in the last year compared to 3% for those scoring low on enacted stigma and high on protective factors. 
Conclusions: Echoing previous findings, this study demonstrates that transgender people across Aotearoa/New Zealand are less likely to manifest life-threatening mental health outcomes if they experience low levels of enacted stigma and high levels of access to protective factors. Our findings suggest a need to address the enacted stigma that transgender people face across interpersonal and structural settings, and also to enhance social supports that are gender affirmative for this population.

year: 2020

title: Romeo and Tusi: An eclectically musical Samoan/Māori Romeo and Juliet from Aotearoa/New Zealand

authors: Houlahan, Mark

subjects: Romeo and Tusi

abstract: Is there a distinctive New Zealand Shakespeare? What ‘new’ approaches to Shakespeare have originated from New Zealand? This article explores the issue by contextualising a version of Romeo and Juliet staged successfully throughout New Zealand in the late 1990s. The production is set against the background of the history of Shakespeare in New Zealand, which goes back to the arrival of Captain Cook on the Endeavour in 1769.

Pacific Underground's Romeo and Tusi sought a looser and more populist kind of Shakespeare performance. They used the conceit of rehearsing the play at high school, where a Māori Romeo and a Samoan Juliet cross community boundaries. Drawing on several music genres, and performing outdoors, they generated a Shakespeare that audiences from a diverse range of ethnic and cultural communities could enjoy.

text: Romeo and Tusi: An eclectically musical Samoan/Māori Romeo and Juliet from Aotearoa/New Zealand Is there a distinctive New Zealand Shakespeare? What ‘new’ approaches to Shakespeare have originated from New Zealand? This article explores the issue by contextualising a version of Romeo and Juliet staged successfully throughout New Zealand in the late 1990s. The production is set against the background of the history of Shakespeare in New Zealand, which goes back to the arrival of Captain Cook on the Endeavour in 1769.

Pacific Underground's Romeo and Tusi sought a looser and more populist kind of Shakespeare performance. They used the conceit of rehearsing the play at high school, where a Māori Romeo and a Samoan Juliet cross community boundaries. Drawing on several music genres, and performing outdoors, they generated a Shakespeare that audiences from a diverse range of ethnic and cultural communities could enjoy.

year: 2009