title: Lake Rotokakahi: The kakahi (Hyridella menziesi) in a general framework of lake health

authors: Butterworth, Joseph

subjects: Rotokakahi

abstract: Lake Rotokakahi is a mesotrophic lake located within the Rotorua Lakes District, North Island, New Zealand. Under the legal guardianship of the Tuhourangi and Ngati Tumatawera tribes of Te Arawa it has remained closed to the public since 1948. 

Lake Rotokakahi was last monitored regularly in 1996 under the Environment Bay of Plenty (EBOP) water quality monitoring programme with only the lake outlet (Te Wairoa Stream) being monitored since that time. Water quality data collected up to 1996 suggests that there may be degradation of water quality in the lake, as indicated by declining levels of dissolved oxygen in the bottom waters.

 Lake Rotokakahi steeped in historical significance, as well as having major cultural and recreational values was well known for its abundant resources and as the name suggests, particularly for its massive supply of the freshwater mussel or kakahi (Hyridella menziesi). Freshwater mussel species worldwide are in decline however little is known on factors controlling kakahi abundance and distribution. 

The overarching objective of this thesis is update water quality data last monitored in Lake Rotokakahi in 1996 while also identifying key environmental variables thought to influence kakahi populations.  This objective is underpinned by a number of aims that include:
 	Establishment of a 12-13 month water quality programme within Lake Rotokakahi so that data is obtained for comparisons with previous water quality data (pre-1997) to allow assessment of whether there have been water quality changes in the lake.
 	Conduct a population survey of the resident kakahi population examining possible environmental factors influencing their populations.
 	Present an oral history of Lake Rotokakahi focusing on its historical significance, water quality, and collection of kairoto (food collected form lakes).

from the 18 September 2006 to 14 September 2007 monthly water sampling was carried out at a mid lake station, the lake outflow and inflow for measures of nutrients, phytoplankton, zooplankton and chlorophyll a. Vertical profiles of temperature dissolved oxygen and chlorophyll fluorescence were also taken on various part of Lake Rotokakahi.

A lengthened period of anoxia in the bottom waters during thermal stratification was recorded with increased levels of chlorophyll a in winter and reduced secchi disk depth indicating an increase in phytoplankton biomass. Nutrient concentrations remain moderate relative to historical data. On going water quality monitoring on Lake Rotokakahi is recommended to further evaluate the extent of which water quality change is occurring. This will provide a better understanding of how Lake Rotokakahi can be best managed to further preserve the lake.

On 1 March and 20 April kakahi were sampled at five sites. Large kakahi densities were distributed predominantly in depths above the hypolimnion. Chlorophyll a fluorescence and dissolved oxygen were found to be the best correlates for kakahi density and biomass respectively. Low dissolved oxygen concentrations in the hypolimnion are thought to restrict kakahi distributions to above the thermocline in periods of hypolimnetic anoxia.

text: Lake Rotokakahi: The kakahi (Hyridella menziesi) in a general framework of lake health Lake Rotokakahi is a mesotrophic lake located within the Rotorua Lakes District, North Island, New Zealand. Under the legal guardianship of the Tuhourangi and Ngati Tumatawera tribes of Te Arawa it has remained closed to the public since 1948. 

Lake Rotokakahi was last monitored regularly in 1996 under the Environment Bay of Plenty (EBOP) water quality monitoring programme with only the lake outlet (Te Wairoa Stream) being monitored since that time. Water quality data collected up to 1996 suggests that there may be degradation of water quality in the lake, as indicated by declining levels of dissolved oxygen in the bottom waters.

 Lake Rotokakahi steeped in historical significance, as well as having major cultural and recreational values was well known for its abundant resources and as the name suggests, particularly for its massive supply of the freshwater mussel or kakahi (Hyridella menziesi). Freshwater mussel species worldwide are in decline however little is known on factors controlling kakahi abundance and distribution. 

The overarching objective of this thesis is update water quality data last monitored in Lake Rotokakahi in 1996 while also identifying key environmental variables thought to influence kakahi populations.  This objective is underpinned by a number of aims that include:
 	Establishment of a 12-13 month water quality programme within Lake Rotokakahi so that data is obtained for comparisons with previous water quality data (pre-1997) to allow assessment of whether there have been water quality changes in the lake.
 	Conduct a population survey of the resident kakahi population examining possible environmental factors influencing their populations.
 	Present an oral history of Lake Rotokakahi focusing on its historical significance, water quality, and collection of kairoto (food collected form lakes).

from the 18 September 2006 to 14 September 2007 monthly water sampling was carried out at a mid lake station, the lake outflow and inflow for measures of nutrients, phytoplankton, zooplankton and chlorophyll a. Vertical profiles of temperature dissolved oxygen and chlorophyll fluorescence were also taken on various part of Lake Rotokakahi.

A lengthened period of anoxia in the bottom waters during thermal stratification was recorded with increased levels of chlorophyll a in winter and reduced secchi disk depth indicating an increase in phytoplankton biomass. Nutrient concentrations remain moderate relative to historical data. On going water quality monitoring on Lake Rotokakahi is recommended to further evaluate the extent of which water quality change is occurring. This will provide a better understanding of how Lake Rotokakahi can be best managed to further preserve the lake.

On 1 March and 20 April kakahi were sampled at five sites. Large kakahi densities were distributed predominantly in depths above the hypolimnion. Chlorophyll a fluorescence and dissolved oxygen were found to be the best correlates for kakahi density and biomass respectively. Low dissolved oxygen concentrations in the hypolimnion are thought to restrict kakahi distributions to above the thermocline in periods of hypolimnetic anoxia.

year: 2008

title: Closing geographical distances: The value of a New Zealand perspective on the admission policy of a Native Hawaiian School

authors: Hemi, Keakaokawai Varner

subjects: Racial discrimination

abstract: This article examines the legal narratives evident in the American federal court decision in Doe v Kamehameha Schools (Kamehameha).' The article first describes the historical development of everyone/ no-one, someone and Indigenous equality narratives and their particular impact on the narration of Indigenous peoples in the law. It then examines how the opinions of the United States Ninth Circuit Court of Appeals in this case exhibit significant narrative confusion and distrust about the relationship between Indigenous identity and equality, with the Ninth Circuit interpreting the Native Hawaiian schools' preference for Native Hawaiian students as either reverse racial discrimination or an exception to the general rule of homogeneity and anonymity. For comparison, the article then examines similar equality narratives in New Zealand law, which reveal few legal challenges to admission policies which, either explicitly or implicitly, prefer Maori and demonstrate a narrative complementarity between equality and Indigenous identity. The article recognises that, while complementarity could clarify the narrative issues in Kamehameha for future American federal courts, it also provides the opportunity for New Zealand to pause and reflect. Ultimately, the article advocates a weaving of equality narratives across geographical distances.

text: Closing geographical distances: The value of a New Zealand perspective on the admission policy of a Native Hawaiian School This article examines the legal narratives evident in the American federal court decision in Doe v Kamehameha Schools (Kamehameha).' The article first describes the historical development of everyone/ no-one, someone and Indigenous equality narratives and their particular impact on the narration of Indigenous peoples in the law. It then examines how the opinions of the United States Ninth Circuit Court of Appeals in this case exhibit significant narrative confusion and distrust about the relationship between Indigenous identity and equality, with the Ninth Circuit interpreting the Native Hawaiian schools' preference for Native Hawaiian students as either reverse racial discrimination or an exception to the general rule of homogeneity and anonymity. For comparison, the article then examines similar equality narratives in New Zealand law, which reveal few legal challenges to admission policies which, either explicitly or implicitly, prefer Maori and demonstrate a narrative complementarity between equality and Indigenous identity. The article recognises that, while complementarity could clarify the narrative issues in Kamehameha for future American federal courts, it also provides the opportunity for New Zealand to pause and reflect. Ultimately, the article advocates a weaving of equality narratives across geographical distances.

year: 2016

title: B cell receptor accessory molecule CD79α: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias)

authors: Li, Ronggai

subjects: cartilaginous fish

abstract: CD79α (also known as Igα) is a component of the B cell antigen receptor complex and plays an important role in B cell signalling. The CD79α protein is present on the surface of B cells throughout their life cycle, and is absent on all other healthy cells, making it a highly reliable marker for B cells in mammals. In this study the spiny dogfish (Squalus acanthias) CD79α (SaCD79α) is described and its expression studied under constitutive and stimulated conditions. The spiny dogfish CD79α cDNA contains an open reading frame of 618 bp, encoding a protein of 205 amino acids. Comparison of the SaCD79α gene with that of other species shows that the gross structure (number of exons, exon/intron boundaries, etc.) is highly conserved across phylogeny. Additionally, analysis of the 5′ flanking region shows SaCD79α lacks a TATA box and possesses binding sites for multiple transcription factors implicated in its B cell-specific gene transcription in other species. Spiny dogfish CD79α is most highly expressed in immune tissues, such as spleen, epigonal and Leydig organ, and its transcript level significantly correlates with those of spiny dogfish immunoglobulin heavy chains. Additionally, CD79α transcription is up-regulated, to a small but significant degree, in peripheral blood cells following stimulation with pokeweed mitogen. These results strongly indicate that, as in mammals, spiny dogfish CD79α is expressed by shark B cells where it associates with surface-bound immunoglobulin to form a fully functional BCR, and thus may serve as a pan-B cell marker in future shark immunological studies.

text: B cell receptor accessory molecule CD79α: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias) CD79α (also known as Igα) is a component of the B cell antigen receptor complex and plays an important role in B cell signalling. The CD79α protein is present on the surface of B cells throughout their life cycle, and is absent on all other healthy cells, making it a highly reliable marker for B cells in mammals. In this study the spiny dogfish (Squalus acanthias) CD79α (SaCD79α) is described and its expression studied under constitutive and stimulated conditions. The spiny dogfish CD79α cDNA contains an open reading frame of 618 bp, encoding a protein of 205 amino acids. Comparison of the SaCD79α gene with that of other species shows that the gross structure (number of exons, exon/intron boundaries, etc.) is highly conserved across phylogeny. Additionally, analysis of the 5′ flanking region shows SaCD79α lacks a TATA box and possesses binding sites for multiple transcription factors implicated in its B cell-specific gene transcription in other species. Spiny dogfish CD79α is most highly expressed in immune tissues, such as spleen, epigonal and Leydig organ, and its transcript level significantly correlates with those of spiny dogfish immunoglobulin heavy chains. Additionally, CD79α transcription is up-regulated, to a small but significant degree, in peripheral blood cells following stimulation with pokeweed mitogen. These results strongly indicate that, as in mammals, spiny dogfish CD79α is expressed by shark B cells where it associates with surface-bound immunoglobulin to form a fully functional BCR, and thus may serve as a pan-B cell marker in future shark immunological studies.

year: 2013