title: The cellulase activity of an extreme thermophile

authors: Hudson, J. Andrew

subjects: biology

abstract: The carboxymethylcellulase activity concentrated from the extremely thermophilic anaerobe H173 was found to have a pH optimum of 6.5–7.0. The enzyme activity was stabilised by the addition of dithiothreitol and CaCl₂•2H₂O and was very stable at 80° C, retaining 77% of the initial activity after 120 min incubtation. At min and after 120 min only 3% of the initial activity remained. With the enzyme dissolved in buffer, glucose and cellobiose were formed from the hydrolosis of Avicel. In culture medium the Avicel-solubilising activity was insensitive to the presence of up tp 50 mm glucose and showed linear glucose accumulation over a period of days at 70° C. HPLC analysis established that glucose was the major end-product of hydrolysis in the culture broths.

text: The cellulase activity of an extreme thermophile The carboxymethylcellulase activity concentrated from the extremely thermophilic anaerobe H173 was found to have a pH optimum of 6.5–7.0. The enzyme activity was stabilised by the addition of dithiothreitol and CaCl₂•2H₂O and was very stable at 80° C, retaining 77% of the initial activity after 120 min incubtation. At min and after 120 min only 3% of the initial activity remained. With the enzyme dissolved in buffer, glucose and cellobiose were formed from the hydrolosis of Avicel. In culture medium the Avicel-solubilising activity was insensitive to the presence of up tp 50 mm glucose and showed linear glucose accumulation over a period of days at 70° C. HPLC analysis established that glucose was the major end-product of hydrolysis in the culture broths.

year: 1991

title: Marine tephrochronology: A personal perspective

authors: Lowe, David J.

abstract: This special volume on marine tephrochronology is remarkable, and timely, because it marks a concerted step towards what might be informally termed ‘phase 3’ of a revolution in Quaternary geosciences that began around 40 years ago. The 10 articles collectively represent a re-focussed examination of tephras and cryptotephras preserved in ocean sediments at various locations and the authors describe their significance for a range of subdisciplines. Eight articles provide a new understanding of the origin, distribution and ages of various tephra and cryptotephra deposits and their stratigraphic inter-relationships; how the terrestrial ages of the tephra/crypotephra deposits relate to those of enclosing sediments and inform the ongoing development of the marine radiocarbon time-scale; mechanisms for the emplacement, remobilization or bioturbation of the tephras or cryptotephras; and volcanic eruption history. Two further articles document the characterization of tephra-derived glass shards using microbeam techniques to analyse 30–40 elements from individual shards as small as 10 µm in diameter. The collection thus provides snapshots of many aspects of the latest developments and directions in tephra studies – volcanology, primary and secondary dispersal, stratigraphy, single-grain characterization, chronology – through the medium of marine sediments. My personal perspective reflects briefly on how this point was reached and identifies a few of the important milestones on the way from ‘phase 1’ to ‘phase 3’. I am privileged to write it. Marine science revolution As an undergraduate in the early-mid 1970s, I recall my first real ‘awakening’ regarding the dynamic nature of science, and of Quaternary geoscience in particular, when told about deep-sea core V28-238 from the equatorial Pacific Ocean (Shackleton & Opdyke 1973; >2650 citations, Google Scholar). Analogous to the opening notes of Beethoven's 5th Symphony, perhaps the most famous quartet of notes in history, the alpha-numerical assemblage ‘V28-2

text: Marine tephrochronology: A personal perspective This special volume on marine tephrochronology is remarkable, and timely, because it marks a concerted step towards what might be informally termed ‘phase 3’ of a revolution in Quaternary geosciences that began around 40 years ago. The 10 articles collectively represent a re-focussed examination of tephras and cryptotephras preserved in ocean sediments at various locations and the authors describe their significance for a range of subdisciplines. Eight articles provide a new understanding of the origin, distribution and ages of various tephra and cryptotephra deposits and their stratigraphic inter-relationships; how the terrestrial ages of the tephra/crypotephra deposits relate to those of enclosing sediments and inform the ongoing development of the marine radiocarbon time-scale; mechanisms for the emplacement, remobilization or bioturbation of the tephras or cryptotephras; and volcanic eruption history. Two further articles document the characterization of tephra-derived glass shards using microbeam techniques to analyse 30–40 elements from individual shards as small as 10 µm in diameter. The collection thus provides snapshots of many aspects of the latest developments and directions in tephra studies – volcanology, primary and secondary dispersal, stratigraphy, single-grain characterization, chronology – through the medium of marine sediments. My personal perspective reflects briefly on how this point was reached and identifies a few of the important milestones on the way from ‘phase 1’ to ‘phase 3’. I am privileged to write it. Marine science revolution As an undergraduate in the early-mid 1970s, I recall my first real ‘awakening’ regarding the dynamic nature of science, and of Quaternary geoscience in particular, when told about deep-sea core V28-238 from the equatorial Pacific Ocean (Shackleton & Opdyke 1973; >2650 citations, Google Scholar). Analogous to the opening notes of Beethoven's 5th Symphony, perhaps the most famous quartet of notes in history, the alpha-numerical assemblage ‘V28-2

year: 2014

title: Ko ngā whakahokinga kōrero a te hunga uiui i roto i te akomanga rumaki i te Whare Wānanga o Waikato: He pūrongo mō ngā ui pātai

authors: Nock, Sophie

subjects: Reo Māori

abstract: Ko te whāinga matua o tēnei rangahau he whakakaokao, he wetewete āno hoki i ngā whakahokinga kōrero a te hunga uiui i kake atu ai ki runga i te waka o Te Tohu Paetahi i te tau 2008, i raro i te kōawa o Rehutai, i raro anō hoki i te maru o Te Whare Wānanga o Waikato. Ka aro atu ki ngā urupare a te hunga tekau mā ono mō te kaupapa nei o Te Tohu Paetahi, ka mutu, ka matapakihia ngā kōrero me ngā whakaaro o taua hunga kia āta kitea ai, āe rānei e tika ana te whakahaere, te mahi whakaako i te reo, te tautoko mai hoki a ngā kaiwhakaako me te kura. I te mutunga iho tautoko katoa te hunga uiui ki aua mea katoa.

text: Ko ngā whakahokinga kōrero a te hunga uiui i roto i te akomanga rumaki i te Whare Wānanga o Waikato: He pūrongo mō ngā ui pātai Ko te whāinga matua o tēnei rangahau he whakakaokao, he wetewete āno hoki i ngā whakahokinga kōrero a te hunga uiui i kake atu ai ki runga i te waka o Te Tohu Paetahi i te tau 2008, i raro i te kōawa o Rehutai, i raro anō hoki i te maru o Te Whare Wānanga o Waikato. Ka aro atu ki ngā urupare a te hunga tekau mā ono mō te kaupapa nei o Te Tohu Paetahi, ka mutu, ka matapakihia ngā kōrero me ngā whakaaro o taua hunga kia āta kitea ai, āe rānei e tika ana te whakahaere, te mahi whakaako i te reo, te tautoko mai hoki a ngā kaiwhakaako me te kura. I te mutunga iho tautoko katoa te hunga uiui ki aua mea katoa.

year: 2017