title: Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from Treponema pallidum

authors: Roberson, Russell S.

subjects: syphilis

abstract: An active pyrophosphate-dependent phosphofructokinase containing a six residue polyhistidine tag has been cloned from Treponema pallidum, and characterized biochemically. The phosphofructokinase has pH optima for activity of 8.0 for both the forward and reverse reactions. The apparent Km for pyrophosphate was 0.042 mM (Vmax of 141 U mg−1 protein) and for fructose-6-phosphate, 0.529 mM. The apparent Km for the reverse reaction for fructose-1,6-diphosphate was 0.267 mM (Vmax of 42.4 U mg−1 protein). The enzyme appears to be both a dimer and non-allosteric.

text: Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from Treponema pallidum An active pyrophosphate-dependent phosphofructokinase containing a six residue polyhistidine tag has been cloned from Treponema pallidum, and characterized biochemically. The phosphofructokinase has pH optima for activity of 8.0 for both the forward and reverse reactions. The apparent Km for pyrophosphate was 0.042 mM (Vmax of 141 U mg−1 protein) and for fructose-6-phosphate, 0.529 mM. The apparent Km for the reverse reaction for fructose-1,6-diphosphate was 0.267 mM (Vmax of 42.4 U mg−1 protein). The enzyme appears to be both a dimer and non-allosteric.

year: 2001

title: Indigenous education: addressing current issues and developments

authors: May, Stephen

subjects: indigenous education

abstract: This introduction to a special issue of Comparative Education focuses on current issues and developments in
both the theory and practice of indigenous education from around the world.

text: Indigenous education: addressing current issues and developments This introduction to a special issue of Comparative Education focuses on current issues and developments in
both the theory and practice of indigenous education from around the world.

year: 2003-04-01

title: From the rākau to the ngākau

authors: Derby, Melissa

subjects: research

abstract: The Ministry of Business, Innovation and Employment (MBIE) in Aotearoa New Zealand has funded 11 National Science Challenges (NSC), which aim to tackle a series of big questions affecting wellbeing in society. One Challenge, A Better Start: E Tipu E Rea, is investigating four key focus areas that children encounter in their early development and is seeking to identify the factors that contribute to forming a solid foundation for fostering wellbeing and lifelong success. Central to the principles of this Challenge is the Treaty of Waitangi in that the Treaty provides a framework to guide whānau engagement, policy change, and praxis. This article describes how a ‘ngākau’ rather than a ‘rākau’ approach to shaping research inquiry within this Challenge facilitates more authentic and mana-enhancing whānau engagement in research interactions (Macfarlane, 2019). The development of a whānau questionnaire is drawn on to illustrate the ngākau approach in practice.

text: From the rākau to the ngākau The Ministry of Business, Innovation and Employment (MBIE) in Aotearoa New Zealand has funded 11 National Science Challenges (NSC), which aim to tackle a series of big questions affecting wellbeing in society. One Challenge, A Better Start: E Tipu E Rea, is investigating four key focus areas that children encounter in their early development and is seeking to identify the factors that contribute to forming a solid foundation for fostering wellbeing and lifelong success. Central to the principles of this Challenge is the Treaty of Waitangi in that the Treaty provides a framework to guide whānau engagement, policy change, and praxis. This article describes how a ‘ngākau’ rather than a ‘rākau’ approach to shaping research inquiry within this Challenge facilitates more authentic and mana-enhancing whānau engagement in research interactions (Macfarlane, 2019). The development of a whānau questionnaire is drawn on to illustrate the ngākau approach in practice.

year: 2021