title: Transitioning from kōhanga reo to kura

authors: Hohepa, Margie Kahukura

abstract: This is a collaborative cross-sector research project involving a kōhanga reo (Māori immersion early childhood language nest) kura (Māori language immersion school) and university-based researchers. It focuses on tamariki (children) moving from kōhanga reo to kura classrooms and will examine the development of an 'akoranga whakawhiti', a transition programme that will be based at Te Kōhanga Reo o Ngā Kuaka in Hamilton. The over arching research question for this project is: Pēhea rā te āhuatanga me te kounga o ngā whakawhitinga mai i te kōhanga ki te kura mō ngā tamariki, whānau, kaiako me te hāpori? - What do effective transitions from kōhanga reo to kura look like, feel like, and sound like, for tamariki, whānau, kaiako and the community? The project aims to provide important new insights into learning and teaching in Māori-medium settings and into ways of enhancing transitions from Maori-medium early childhood education to Māori medium classrooms.

text: Transitioning from kōhanga reo to kura This is a collaborative cross-sector research project involving a kōhanga reo (Māori immersion early childhood language nest) kura (Māori language immersion school) and university-based researchers. It focuses on tamariki (children) moving from kōhanga reo to kura classrooms and will examine the development of an 'akoranga whakawhiti', a transition programme that will be based at Te Kōhanga Reo o Ngā Kuaka in Hamilton. The over arching research question for this project is: Pēhea rā te āhuatanga me te kounga o ngā whakawhitinga mai i te kōhanga ki te kura mō ngā tamariki, whānau, kaiako me te hāpori? - What do effective transitions from kōhanga reo to kura look like, feel like, and sound like, for tamariki, whānau, kaiako and the community? The project aims to provide important new insights into learning and teaching in Māori-medium settings and into ways of enhancing transitions from Maori-medium early childhood education to Māori medium classrooms.

year: 2014

title: 'Thinking-through-Complicity' with Te Iwi o Ngāti Hauiti: Towards a Critical Use of Participatory Video for Research

authors: Kindon, Sara Louise

subjects: human geography

abstract: This thesis explores some of the seductions and dangers of participatory video for research (PVR) involving Indigenous Māori and Pākehā research partners. The project within which PVR was used focused on exploring relationships between place, identity and social cohesion within ‘remote’ rural communities. It involved about 15 members of the Potaka whānau of Te Iwi o Ngāti Hauiti in the central Rangitīkei district of the North Island, Aotearoa New Zealand. A small group of iwi members, myself and an audiovisual specialist and trainer negotiated the project’s focus, process and ethics during 1998. A different group of iwi members were then trained in video production and community research methods later that year and supported to produce their own productions, and carry out video research interviews with other iwi members. The entire process of negotiation, training and collaborative research was filmed for archival and research purposes with everyone’s consent, and several collaborative publications and presentations have been produced since 1999.

The discursive space opened up by Ngāti Hauiti’s engagement with, and use of, video provides an opportunity to attend to the ‘cultural mediations’ that occurred throughout the research partnership and to inquire into the possible ‘empire building effects’ of visual technologies within participatory research more generally. The focus on PVR within a Māori context also prompts questions about the visual’s transformative potential within geographic research, and the implications of working through the use of a visual medium for rethinking disciplinary practices and knowledges, particularly when working cross-culturally. 

In the thesis, I first review the evolution and attendant challenges associated with both the use of participation and video within research contexts. I trace their similar origins in modernist attempts to ‘know’ and ‘empower’ marginalised others, and highlight the ongoing marginalisation of Indigenous perspectives within mainstream debates. I then engage with conceptualisations of complicity and develop an analytical framework that expands on current discursive and ideological discussions to also attend to its material, embodied and spatial dimensions.

Using this framework and a complementary autoethnographic and ‘hyper-self-reflexive’ approach, I track aspects of my own power, complicity and desire within my research practice in the PVR project during the period 1998-2001. This approach involves the development of a particular reading position to focus on critical incidents of my research practice and a means of grappling productively with the polyvalent nature of my audiovisual and other information sources. I discuss these critical incidents within three processes associated with the research: facilitation, production and reception, attending to the complex and multifaceted interplay of audiovisual texts, their producers and their audiences throughout. 

Such a thesis is expedient given that powerful and often uncritical rhetoric that besets participatory research and development is fast taking hold within geography. It is also timely given the proliferation of affordable and accessible audiovisual technology and its increasing use within geography and other social sciences. As geographers respond to calls to embrace more visual, tactile and other methods, this thesis offers possibilities for the repoliticisation of participatory discourse within social geography, through a more considered engagement with participatory action research, Indigenous research practices and audiovisual media such as video. I offer cautionary insights into the ‘power-full’ effects of these ways of working.

text: 'Thinking-through-Complicity' with Te Iwi o Ngāti Hauiti: Towards a Critical Use of Participatory Video for Research This thesis explores some of the seductions and dangers of participatory video for research (PVR) involving Indigenous Māori and Pākehā research partners. The project within which PVR was used focused on exploring relationships between place, identity and social cohesion within ‘remote’ rural communities. It involved about 15 members of the Potaka whānau of Te Iwi o Ngāti Hauiti in the central Rangitīkei district of the North Island, Aotearoa New Zealand. A small group of iwi members, myself and an audiovisual specialist and trainer negotiated the project’s focus, process and ethics during 1998. A different group of iwi members were then trained in video production and community research methods later that year and supported to produce their own productions, and carry out video research interviews with other iwi members. The entire process of negotiation, training and collaborative research was filmed for archival and research purposes with everyone’s consent, and several collaborative publications and presentations have been produced since 1999.

The discursive space opened up by Ngāti Hauiti’s engagement with, and use of, video provides an opportunity to attend to the ‘cultural mediations’ that occurred throughout the research partnership and to inquire into the possible ‘empire building effects’ of visual technologies within participatory research more generally. The focus on PVR within a Māori context also prompts questions about the visual’s transformative potential within geographic research, and the implications of working through the use of a visual medium for rethinking disciplinary practices and knowledges, particularly when working cross-culturally. 

In the thesis, I first review the evolution and attendant challenges associated with both the use of participation and video within research contexts. I trace their similar origins in modernist attempts to ‘know’ and ‘empower’ marginalised others, and highlight the ongoing marginalisation of Indigenous perspectives within mainstream debates. I then engage with conceptualisations of complicity and develop an analytical framework that expands on current discursive and ideological discussions to also attend to its material, embodied and spatial dimensions.

Using this framework and a complementary autoethnographic and ‘hyper-self-reflexive’ approach, I track aspects of my own power, complicity and desire within my research practice in the PVR project during the period 1998-2001. This approach involves the development of a particular reading position to focus on critical incidents of my research practice and a means of grappling productively with the polyvalent nature of my audiovisual and other information sources. I discuss these critical incidents within three processes associated with the research: facilitation, production and reception, attending to the complex and multifaceted interplay of audiovisual texts, their producers and their audiences throughout. 

Such a thesis is expedient given that powerful and often uncritical rhetoric that besets participatory research and development is fast taking hold within geography. It is also timely given the proliferation of affordable and accessible audiovisual technology and its increasing use within geography and other social sciences. As geographers respond to calls to embrace more visual, tactile and other methods, this thesis offers possibilities for the repoliticisation of participatory discourse within social geography, through a more considered engagement with participatory action research, Indigenous research practices and audiovisual media such as video. I offer cautionary insights into the ‘power-full’ effects of these ways of working.

year: 2012

title: Characterization of the potential drug target serine acetyltransferase (CysE) from Acinetobacter baumannii: A Pacific lens on biochemical research

authors: Usu, Jessicca Eva

abstract: Antimicrobial resistance (AMR) presents a critical global health threat, with Acinetobacter baumannii being a key pathogen due to its multidrug resistance. With no new antibiotic classes discovered since the 1980s, novel therapeutic targets are urgently needed. This thesis explores the potential of targeting the cysteine biosynthesis pathway, specifically the enzyme serine acetyltransferase (CysE), as a means to develop innovative antimicrobial strategies. Cysteine plays a pivotal role in bacterial survival and defense, particularly in oxidative stress resistance. Targeting its biosynthesis may impair bacterial virulence and enhance susceptibility to immune responses.

This research focuses on the characterization of CysE from A. baumannii, which uniquely expresses a short and full-length of this enzyme. While we were unable to optimize expression of the short form in this thesis, the long form was successfully expressed, purified, and biochemically and structurally characterized for the first time. Using X-ray crystallography, we present the three-dimensional structure of CysE from A. baumannii to a resolution of 2.14 Å. CysE adopts a homohexameric conformation, forming a dimer of trimers, and features an alpha-helical extension. SAXS analysis validated that the long form exists as a homohexamer in solution. Furthermore, using enzymatic assays we show CysE has serine acetyltransferase activity and is sensitive to feedback inhibition by pathway product L-cysteine, highlighting the regulatory mechanisms of this crucial enzyme. This work improves our understanding of cysteine biosynthesis in A. baumannii and provides a model for structure-based virtual inhibitor screening to identify potential new antimicrobials. 

In addition to its scientific objectives, this research is framed within the Fa'afaletui framework, an Indigenous Samoan methodology that ensures cultural perspectives are integrated into the scientific process. This approach ensures that Pacific values inform the direction of AMR research, fostering solutions that are not only scientifically rigorous but are also culturally relevant and accessible. The research serves to address the health inequities faced by Pacific peoples, particularly in relation to infectious diseases and AMR, and provides a foundation for future antimicrobial drug development efforts.

text: Characterization of the potential drug target serine acetyltransferase (CysE) from Acinetobacter baumannii: A Pacific lens on biochemical research Antimicrobial resistance (AMR) presents a critical global health threat, with Acinetobacter baumannii being a key pathogen due to its multidrug resistance. With no new antibiotic classes discovered since the 1980s, novel therapeutic targets are urgently needed. This thesis explores the potential of targeting the cysteine biosynthesis pathway, specifically the enzyme serine acetyltransferase (CysE), as a means to develop innovative antimicrobial strategies. Cysteine plays a pivotal role in bacterial survival and defense, particularly in oxidative stress resistance. Targeting its biosynthesis may impair bacterial virulence and enhance susceptibility to immune responses.

This research focuses on the characterization of CysE from A. baumannii, which uniquely expresses a short and full-length of this enzyme. While we were unable to optimize expression of the short form in this thesis, the long form was successfully expressed, purified, and biochemically and structurally characterized for the first time. Using X-ray crystallography, we present the three-dimensional structure of CysE from A. baumannii to a resolution of 2.14 Å. CysE adopts a homohexameric conformation, forming a dimer of trimers, and features an alpha-helical extension. SAXS analysis validated that the long form exists as a homohexamer in solution. Furthermore, using enzymatic assays we show CysE has serine acetyltransferase activity and is sensitive to feedback inhibition by pathway product L-cysteine, highlighting the regulatory mechanisms of this crucial enzyme. This work improves our understanding of cysteine biosynthesis in A. baumannii and provides a model for structure-based virtual inhibitor screening to identify potential new antimicrobials. 

In addition to its scientific objectives, this research is framed within the Fa'afaletui framework, an Indigenous Samoan methodology that ensures cultural perspectives are integrated into the scientific process. This approach ensures that Pacific values inform the direction of AMR research, fostering solutions that are not only scientifically rigorous but are also culturally relevant and accessible. The research serves to address the health inequities faced by Pacific peoples, particularly in relation to infectious diseases and AMR, and provides a foundation for future antimicrobial drug development efforts.

year: 2024