title: Economical genotyping of little blue penguin (Eudyptula minor) clades from feather-based DNA

authors: Clark, Alan J.

subjects: Clades

abstract: Determination of clade membership is a crucial requirement for many research questions addressing phylogeography, population structure, mating patterns, speciation, and hybridisation. The little blue penguin (Eudyptula minor) can be separated into two deeply divergent clades. However, assigning clade membership in little blue penguins requires molecular methods. Genetic sequencing can be used to identify clade membership but is expensive. Here, we present an economical alternative to the use of sequencing to determine little blue penguin clade membership. We extracted DNA from feathers using a method that produced reasonable quantities of DNA. We then amplified the D-loop section of the mitochondrial control region from total genomic DNA extracts, using the primers 'C L-tRNAglu' and 'D H-Dbox' followed by digestion with the restriction enzyme AluI. When visualised on a gel, distinctive banding patterns clearly indicated clade membership. We sequenced a subset of our samples and verified the accuracy of this method. The methods we present should facilitate little blue penguin research through a cost-effective approach to clade analysis as well as a successful technique to extract DNA from feathers when blood or tissue samples are not available.

text: Economical genotyping of little blue penguin (Eudyptula minor) clades from feather-based DNA Determination of clade membership is a crucial requirement for many research questions addressing phylogeography, population structure, mating patterns, speciation, and hybridisation. The little blue penguin (Eudyptula minor) can be separated into two deeply divergent clades. However, assigning clade membership in little blue penguins requires molecular methods. Genetic sequencing can be used to identify clade membership but is expensive. Here, we present an economical alternative to the use of sequencing to determine little blue penguin clade membership. We extracted DNA from feathers using a method that produced reasonable quantities of DNA. We then amplified the D-loop section of the mitochondrial control region from total genomic DNA extracts, using the primers 'C L-tRNAglu' and 'D H-Dbox' followed by digestion with the restriction enzyme AluI. When visualised on a gel, distinctive banding patterns clearly indicated clade membership. We sequenced a subset of our samples and verified the accuracy of this method. The methods we present should facilitate little blue penguin research through a cost-effective approach to clade analysis as well as a successful technique to extract DNA from feathers when blood or tissue samples are not available.

year: 2013

title: Three Illuminated Videos

authors: Barrett, Kyle

abstract: Kyle Barrett, “Illuminated: Antisyzygy” (8:40) — Blending film and painting, the video postulates several questions arising from the COVID-19 pandemic via the notion of “antisyzygy” or the in-between state in which, globally, society has found itself. Ilona Krawczyk, “More than one voice” (10:00) — Why do I feel connected to something which is beyond me, which comes from the past, which I don’t understand? But there are those moments of unconsciousness that come out. Charlotta Grimfjord Cederblad, “Preparation” (10:17) — This video is an observation of what we prepare for when we prepare for performance, of how we prepare for the fact that we soon will be watched, of how we imagine that we are being watched and of what happens with the body when we think that no one is watching.

text: Three Illuminated Videos Kyle Barrett, “Illuminated: Antisyzygy” (8:40) — Blending film and painting, the video postulates several questions arising from the COVID-19 pandemic via the notion of “antisyzygy” or the in-between state in which, globally, society has found itself. Ilona Krawczyk, “More than one voice” (10:00) — Why do I feel connected to something which is beyond me, which comes from the past, which I don’t understand? But there are those moments of unconsciousness that come out. Charlotta Grimfjord Cederblad, “Preparation” (10:17) — This video is an observation of what we prepare for when we prepare for performance, of how we prepare for the fact that we soon will be watched, of how we imagine that we are being watched and of what happens with the body when we think that no one is watching.

year: 2021

title: Viewing restorative approaches to addressing challenging behaviour of minority ethnic students through a community of practice lens

authors: Wearmouth, Janice

subjects: restorative practice

abstract: The disproportionately high rates of school exclusion and lower levels of academic achievement of students from particular minority ethnic groups have been a focus of investigation in educational research across the world for some time. This articles uses a communities of practice framework to examine how restorative practice can draw on family and community values to support students whose behaviour is unacceptable in schools to recognize their own agency in behaving for good or ill and reintegrate them into the school community. At the same time, it acknowledges hurt that may be done to victims of wrong-doing and emphasizes putting things right between all those affected. The examples here are from Aotearoa New Zealand. They relate to practices influenced by traditional Māori cultural values. However, the principles and process associated with restorative practices that aim to restore harmony between the individual, the victim and the collective rather than to punish and exclude may be relevant within other student groups where high rates of exclusion from school are problematic.

text: Viewing restorative approaches to addressing challenging behaviour of minority ethnic students through a community of practice lens The disproportionately high rates of school exclusion and lower levels of academic achievement of students from particular minority ethnic groups have been a focus of investigation in educational research across the world for some time. This articles uses a communities of practice framework to examine how restorative practice can draw on family and community values to support students whose behaviour is unacceptable in schools to recognize their own agency in behaving for good or ill and reintegrate them into the school community. At the same time, it acknowledges hurt that may be done to victims of wrong-doing and emphasizes putting things right between all those affected. The examples here are from Aotearoa New Zealand. They relate to practices influenced by traditional Māori cultural values. However, the principles and process associated with restorative practices that aim to restore harmony between the individual, the victim and the collective rather than to punish and exclude may be relevant within other student groups where high rates of exclusion from school are problematic.

year: 2012