title: Investigating molecular approaches for detection of pathogens in horses

authors: Grout, Emily Katherine

subjects: Polymerase chain reaction

abstract: Infectious disease of equines has significant impacts on the New Zealand equine industry, causing avoidable morbidity and mortality. The correct identification of the aetiological agents involved in such infections allows for prompt and accurate administration of an appropriate therapeutic strategy, reducing the economic impact associated with equine wastage.  
 
Traditional culturing methods are widely used for the diagnosis of infections in horses, however, conventional PCR offers a novel and rapid approach, through the targeting of unique genes for diagnosis of pathogens. PCR is an attractive alternative to microbial culturing, which can take anywhere between 24 hours to 1 week to determine the causative infectious agent, and in a number of cases the results are not as specific or as accurate as they need to be. It is the lack of sensitivity of the traditional culturing techniques that continues to contribute to the economic loss and welfare issues associated with equine infections.  
 
Initially, it was found that an SDS lysis extraction method worked best for extraction of DNA which was used to extract microbial DNA from various types of clinical samples. These were subsequently used as DNA templates within PCR alongside primers associated with up to 28 genes associated with common equine infections. From a total 146 clinical samples received for testing 66.4% returned a PCR positive for one or more microbial genera, whereas traditional bacteriological culturing produced an identification rate of 46%. In addition, PCR was significantly faster than culturing methods (6 hours vs 48 hours) and allowed more specific identification of the microbes present, which provides a significant advantage for infection management and increasing animal welfare. 
 
The results found from these experiments show rapid PCR identification holds promise as a diagnostic technique in the future, however, further development is required before this can be used as a primary diagnostic technique. Recommendations include further research into trends of infection in NZ equines and respectively increasing the diversity of PCR primers to represent the larger scope of microbial species. PCR diagnosis would also benefit from the standardisation of the DNA extraction methods used to allow consistency and increase throughput of various samples. Lastly, the simplification of the individual PCRs for each infectious agent could be developed into multiplex reactions to decrease time processing time and reduce human pipetting error.

text: Investigating molecular approaches for detection of pathogens in horses Infectious disease of equines has significant impacts on the New Zealand equine industry, causing avoidable morbidity and mortality. The correct identification of the aetiological agents involved in such infections allows for prompt and accurate administration of an appropriate therapeutic strategy, reducing the economic impact associated with equine wastage.  
 
Traditional culturing methods are widely used for the diagnosis of infections in horses, however, conventional PCR offers a novel and rapid approach, through the targeting of unique genes for diagnosis of pathogens. PCR is an attractive alternative to microbial culturing, which can take anywhere between 24 hours to 1 week to determine the causative infectious agent, and in a number of cases the results are not as specific or as accurate as they need to be. It is the lack of sensitivity of the traditional culturing techniques that continues to contribute to the economic loss and welfare issues associated with equine infections.  
 
Initially, it was found that an SDS lysis extraction method worked best for extraction of DNA which was used to extract microbial DNA from various types of clinical samples. These were subsequently used as DNA templates within PCR alongside primers associated with up to 28 genes associated with common equine infections. From a total 146 clinical samples received for testing 66.4% returned a PCR positive for one or more microbial genera, whereas traditional bacteriological culturing produced an identification rate of 46%. In addition, PCR was significantly faster than culturing methods (6 hours vs 48 hours) and allowed more specific identification of the microbes present, which provides a significant advantage for infection management and increasing animal welfare. 
 
The results found from these experiments show rapid PCR identification holds promise as a diagnostic technique in the future, however, further development is required before this can be used as a primary diagnostic technique. Recommendations include further research into trends of infection in NZ equines and respectively increasing the diversity of PCR primers to represent the larger scope of microbial species. PCR diagnosis would also benefit from the standardisation of the DNA extraction methods used to allow consistency and increase throughput of various samples. Lastly, the simplification of the individual PCRs for each infectious agent could be developed into multiplex reactions to decrease time processing time and reduce human pipetting error.

year: 2018

title: Infinite order symmetries for two-dimensional separable Schrödinger equations

authors: Kalnins, Ernie G.

subjects: mathematics

abstract: Consider a non-relativistic Hamiltonian operator H in 2 dimensions consisting of a kinetic energy term plus a potential. We show that if the associated Schrödinger eigenvalue equation admits an orthogonal separation of variables, there is a calculus to describe the (in general) infinite-order differential operator symmetries of the Schrödinger equation. The calculus is formal but can be made rigorous when all functions in the eigenvaue equation are analytic. The infinite-order calculus exhibits structure that is not apparent when one studies only finite-order symmetries. The search for finite-order symmetries can then be reposed as one of looking for solutions of a coupled system of PDEs that are polynomial in certain parameters. We go further and extend the calculus to the situation where the Schrödinger equation admits a second-order symmetry operator, not necessarily associated with orthogonal separable coordinates.

text: Infinite order symmetries for two-dimensional separable Schrödinger equations Consider a non-relativistic Hamiltonian operator H in 2 dimensions consisting of a kinetic energy term plus a potential. We show that if the associated Schrödinger eigenvalue equation admits an orthogonal separation of variables, there is a calculus to describe the (in general) infinite-order differential operator symmetries of the Schrödinger equation. The calculus is formal but can be made rigorous when all functions in the eigenvaue equation are analytic. The infinite-order calculus exhibits structure that is not apparent when one studies only finite-order symmetries. The search for finite-order symmetries can then be reposed as one of looking for solutions of a coupled system of PDEs that are polynomial in certain parameters. We go further and extend the calculus to the situation where the Schrödinger equation admits a second-order symmetry operator, not necessarily associated with orthogonal separable coordinates.

year: 2004

title: Ko ia tonu te rangatira o tana mauri: Ko te Mauritau hei tohu o te puāwaitanga o Te Aho Matua i te Kura Kaupapa Māori o Hoani Waititi Marae

authors: Angeli-Gordon, Joni Māramatanga

abstract: Ko te mauri te wāhanga wairua o te tangata e whakahihiri i te tinana me te tūhonohono nei i te wairua ki te kikokiko. Ko te mauri te wāhanga ora o te tangata, ā, i te roanga atu o te oranga o te tangata ka rerekē te tau o te mauri nā ngā awenga o tōna ao. Ko te Mauritau te wā e ita pai ana te wairua ki te kikokiko. Kua whai ngātahitanga ērā mea e rua. Kei tōna ito, ki te Mauritau te tangata, e wātea ana ia, kāhore ōna taumaha, ka mutu e tuwhera ana ia ki tōna ao. Ko tā Te Aho Matua, ka whakatō te mauri ki te tangata i te whakatōnga o te kākano ki te kōpū. Ko te Mauritau tētahi āhuatanga kāhore anō kia tino rangahau, ā, he iti noa ngā wā te kite atu ki te puna mātātuhi, heoi anō, ka rangona whānuitia ki Te Kura Kaupapa Māori o Hoani Waititi Marae. Ko tā tēnei kaupapa rangahau, ko te Mauritau tētahi o ngā puāwaitanga matua o Te Aho Matua, ā, kua taupuhipuhi nei ki ngā whakaaro o rātou i whai wāhi nei ki tēnei kaupapa rangahau. Ko te manako nui o tēnei kaupapa rangahau ko te whakawhānui i te mōhioranga mō ngā hua o ngā Kura Kaupapa Māori mā te ruku hōhonu ki te Mauritau i te whānau o Te Kura Kaupapa Māori o Hoani Waititi Marae.  

Kua whā tekau tau neke atu kua hipa mai i te orokotīmatanga o ngā Kura Kaupapa Māori, otirā, te Kura Kaupapa Māori o Hoani Waititi Marae. Ko te tino take o ngā Kura Kaupapa Māori ko te whakarauora reo me te whakarauora tikanga, heoi anō, he tini me te mano ngā angitutanga, ngā painga kua puta mai i ēnei kura tua atu i ēnei whāinga e rua. Ko Te Aho Matua te tūāpapa ā-mātāpono mō ngā Kura Kaupapa Māori e whakawhārikihia ngā uaratanga matua kia whakatutuki i ngā wawata nui, i ngā ōhākī nui ā kui mā, a koro mā mō te orangatonutanga o te reo Māori me ngā tikanga Māori. Ko tā tēnei kaupapa rangahau, ko te Mauritau tētahi āhuatanga i puāwai i ngā kura Aho Matua, ā, ka kitea rawatia tēnei puāwaitanga ki Te Kura Kaupapa Māori o Hoani Waititi. 

He Rangahau Kaupapa Māori tēnei, ā, i tārai i ngā tikanga me ngā tukanga o te kaupapa rangahau nei kia Māori te karawhiu. Ko Te Aho Matua me ōna uaratanga e tū hei pou whakawhirinaki i te Kaupapa Rangahau Māori. E rua ngā tukanga i whāia e te tuhikura nei, arā, ko te iāna me te puawānanga. Ko te iāna tētahi kupu mino nō Te Tai Tokerau mō te kōrero ōpaki, ā, kua iāna tahi ki ngā pakeke tokowhitu nō te whānau o Te Kura Kaupapa Māori o Hoani Waititi Marae. Ko te puawānanga he wānanga ki ngā ākonga tokowhitu o te reanga tuakana o te wharekura kia āta wherawhera i ngā āhuatanga o te Mauritau ki a rātou. I te whakaraupapatia ngā kōrero i puta mai i ngā iāna me te puawānanga mā ngā uaratanga o Te Aho Matua.

text: Ko ia tonu te rangatira o tana mauri: Ko te Mauritau hei tohu o te puāwaitanga o Te Aho Matua i te Kura Kaupapa Māori o Hoani Waititi Marae Ko te mauri te wāhanga wairua o te tangata e whakahihiri i te tinana me te tūhonohono nei i te wairua ki te kikokiko. Ko te mauri te wāhanga ora o te tangata, ā, i te roanga atu o te oranga o te tangata ka rerekē te tau o te mauri nā ngā awenga o tōna ao. Ko te Mauritau te wā e ita pai ana te wairua ki te kikokiko. Kua whai ngātahitanga ērā mea e rua. Kei tōna ito, ki te Mauritau te tangata, e wātea ana ia, kāhore ōna taumaha, ka mutu e tuwhera ana ia ki tōna ao. Ko tā Te Aho Matua, ka whakatō te mauri ki te tangata i te whakatōnga o te kākano ki te kōpū. Ko te Mauritau tētahi āhuatanga kāhore anō kia tino rangahau, ā, he iti noa ngā wā te kite atu ki te puna mātātuhi, heoi anō, ka rangona whānuitia ki Te Kura Kaupapa Māori o Hoani Waititi Marae. Ko tā tēnei kaupapa rangahau, ko te Mauritau tētahi o ngā puāwaitanga matua o Te Aho Matua, ā, kua taupuhipuhi nei ki ngā whakaaro o rātou i whai wāhi nei ki tēnei kaupapa rangahau. Ko te manako nui o tēnei kaupapa rangahau ko te whakawhānui i te mōhioranga mō ngā hua o ngā Kura Kaupapa Māori mā te ruku hōhonu ki te Mauritau i te whānau o Te Kura Kaupapa Māori o Hoani Waititi Marae.  

Kua whā tekau tau neke atu kua hipa mai i te orokotīmatanga o ngā Kura Kaupapa Māori, otirā, te Kura Kaupapa Māori o Hoani Waititi Marae. Ko te tino take o ngā Kura Kaupapa Māori ko te whakarauora reo me te whakarauora tikanga, heoi anō, he tini me te mano ngā angitutanga, ngā painga kua puta mai i ēnei kura tua atu i ēnei whāinga e rua. Ko Te Aho Matua te tūāpapa ā-mātāpono mō ngā Kura Kaupapa Māori e whakawhārikihia ngā uaratanga matua kia whakatutuki i ngā wawata nui, i ngā ōhākī nui ā kui mā, a koro mā mō te orangatonutanga o te reo Māori me ngā tikanga Māori. Ko tā tēnei kaupapa rangahau, ko te Mauritau tētahi āhuatanga i puāwai i ngā kura Aho Matua, ā, ka kitea rawatia tēnei puāwaitanga ki Te Kura Kaupapa Māori o Hoani Waititi. 

He Rangahau Kaupapa Māori tēnei, ā, i tārai i ngā tikanga me ngā tukanga o te kaupapa rangahau nei kia Māori te karawhiu. Ko Te Aho Matua me ōna uaratanga e tū hei pou whakawhirinaki i te Kaupapa Rangahau Māori. E rua ngā tukanga i whāia e te tuhikura nei, arā, ko te iāna me te puawānanga. Ko te iāna tētahi kupu mino nō Te Tai Tokerau mō te kōrero ōpaki, ā, kua iāna tahi ki ngā pakeke tokowhitu nō te whānau o Te Kura Kaupapa Māori o Hoani Waititi Marae. Ko te puawānanga he wānanga ki ngā ākonga tokowhitu o te reanga tuakana o te wharekura kia āta wherawhera i ngā āhuatanga o te Mauritau ki a rātou. I te whakaraupapatia ngā kōrero i puta mai i ngā iāna me te puawānanga mā ngā uaratanga o Te Aho Matua.

year: 2023