title: The thermal behaviour of enzyme activity: implications for biotechnology

authors: Eisenthal, Robert

subjects: enzyme

abstract: The way that enzymes respond to temperature is fundamental to many areas of biotechnology. This has long been explained in terms of enzyme stability and catalytic activation energy, but recent observations of enzyme behaviour suggest that this picture is incomplete. We have developed and experimentally validated a new model to describe the effect of temperature on enzymes; this model incorporates additional fundamental parameters that enable a complete description of the effects of temperature on enzyme activity. In this article, we consider the biotechnological implications of this model in the areas of enzyme engineering, enzyme reactor operation and the selection and/or screening of useful enzymes from the environment.

text: The thermal behaviour of enzyme activity: implications for biotechnology The way that enzymes respond to temperature is fundamental to many areas of biotechnology. This has long been explained in terms of enzyme stability and catalytic activation energy, but recent observations of enzyme behaviour suggest that this picture is incomplete. We have developed and experimentally validated a new model to describe the effect of temperature on enzymes; this model incorporates additional fundamental parameters that enable a complete description of the effects of temperature on enzyme activity. In this article, we consider the biotechnological implications of this model in the areas of enzyme engineering, enzyme reactor operation and the selection and/or screening of useful enzymes from the environment.

year: 2006

title: A spatially resolved model of seasonal variations in phytoplankton and clam (Tapes philippinarum) biomass in Barbamarco Lagoon, Italy

authors: Spillman, C.M.

subjects: tapes philippinarum

abstract: Barbamarco Lagoon (area = 7 km²) is in the Po River Delta, adjoining the Northern Adriatic Sea, and supports a commercially valuable clam (Tapes philippinarum) fishery. This study investigated interactions of the lagoon with adjacent coastal waters and inland riverine inputs by modelling both the lagoon and the Northern Adriatic Sea, using a coupled three-dimensional (3D) hydrodynamic-ecological model (ELCOM-CAEDYM) adapted to include the clam population. The clam model accounted for carbon (C), nitrogen (N) and phosphorus (P) biomass in the benthos through parameterisations for filtration, excretion, egestion, respiration, mortality, and harvesting. Multiple clam size classes were included in a new population dynamics sub-model. Output from the coupled model was validated against hydrodynamic and water quality data from intensive field sampling and routine monitoring. Time scales of tidal flushing, primary production and clam grazing were investigated with the model to demonstrate that food supply to clam populations is dominated by phytoplankton inputs from the Northern Adriatic Sea. Effects of clam cultivation on nutrient concentrations and phytoplankton biomass in Barbamarco Lagoon were primarily localised, with strong tidal flushing minimising impacts of clam filtration on lagoon-wide nutrient concentrations at current clam stocking levels. Clam populations were found to alter the cycling of nutrients in the system, causing the lagoon to become a net sink for particulate organic matter and to export dissolved organic matter to the adjacent sea via tidal flushing. Ecosystem health and sensitivity of nutrient cycles to clam cultivation are important considerations for the long term sustainable management and potential expansion of the fishery.

text: A spatially resolved model of seasonal variations in phytoplankton and clam (Tapes philippinarum) biomass in Barbamarco Lagoon, Italy Barbamarco Lagoon (area = 7 km²) is in the Po River Delta, adjoining the Northern Adriatic Sea, and supports a commercially valuable clam (Tapes philippinarum) fishery. This study investigated interactions of the lagoon with adjacent coastal waters and inland riverine inputs by modelling both the lagoon and the Northern Adriatic Sea, using a coupled three-dimensional (3D) hydrodynamic-ecological model (ELCOM-CAEDYM) adapted to include the clam population. The clam model accounted for carbon (C), nitrogen (N) and phosphorus (P) biomass in the benthos through parameterisations for filtration, excretion, egestion, respiration, mortality, and harvesting. Multiple clam size classes were included in a new population dynamics sub-model. Output from the coupled model was validated against hydrodynamic and water quality data from intensive field sampling and routine monitoring. Time scales of tidal flushing, primary production and clam grazing were investigated with the model to demonstrate that food supply to clam populations is dominated by phytoplankton inputs from the Northern Adriatic Sea. Effects of clam cultivation on nutrient concentrations and phytoplankton biomass in Barbamarco Lagoon were primarily localised, with strong tidal flushing minimising impacts of clam filtration on lagoon-wide nutrient concentrations at current clam stocking levels. Clam populations were found to alter the cycling of nutrients in the system, causing the lagoon to become a net sink for particulate organic matter and to export dissolved organic matter to the adjacent sea via tidal flushing. Ecosystem health and sensitivity of nutrient cycles to clam cultivation are important considerations for the long term sustainable management and potential expansion of the fishery.

year: 2011

title: ‘Revolting murder at Te Aroha’ in 1881

authors: Hart, Philip

abstract: The brutal murder of Hamiona Haira, who had been mining with his two brothers and his father-in-law, shocked the new settlement. Being a member of Ngati Koi and Ngati Hako, these hapu threatened utu on the Pakeha responsible, causing both Maori and Pakeha miners to abandon their claims at Tui. Suspicion immediately fell on John Procoffy, a Finn, and evidence was quickly collected, officials being anxious to obtain a speedy conviction to avoid an innocent Pakeha being killed in revenge. Rangatira agreed to let the courts deal with the case, although some Pakeha criticized the government for being too anxious to appease Maori.
After a coroner’s inquest, which included Maori as members of the jury, returned a verdict of murder by ‘person or persons unknown’, Procoffy faced two trials. As the prosecution was handicapped by the limitations of contemporary forensic skills, its case was largely circumstantial. Although the police were certain they had their murderer, others were not convinced, and there was a reluctance to convict because of the death penalty. The final outcome was a verdict of not guilty, which, as was pointed out, should have been ‘not proven’. 
Procoffy fled the country. His acquittal was accepted by Maori leaders, and calm returned to Te Aroha.

text: ‘Revolting murder at Te Aroha’ in 1881 The brutal murder of Hamiona Haira, who had been mining with his two brothers and his father-in-law, shocked the new settlement. Being a member of Ngati Koi and Ngati Hako, these hapu threatened utu on the Pakeha responsible, causing both Maori and Pakeha miners to abandon their claims at Tui. Suspicion immediately fell on John Procoffy, a Finn, and evidence was quickly collected, officials being anxious to obtain a speedy conviction to avoid an innocent Pakeha being killed in revenge. Rangatira agreed to let the courts deal with the case, although some Pakeha criticized the government for being too anxious to appease Maori.
After a coroner’s inquest, which included Maori as members of the jury, returned a verdict of murder by ‘person or persons unknown’, Procoffy faced two trials. As the prosecution was handicapped by the limitations of contemporary forensic skills, its case was largely circumstantial. Although the police were certain they had their murderer, others were not convinced, and there was a reluctance to convict because of the death penalty. The final outcome was a verdict of not guilty, which, as was pointed out, should have been ‘not proven’. 
Procoffy fled the country. His acquittal was accepted by Maori leaders, and calm returned to Te Aroha.

year: 2016