title: Influence of nitrogen on the primary and secondary metabolism and synthesis of flavonoids in Chrysanthemum morifolium Ramat

authors: Liu, Wei

subjects: Chrysanthemum moriforlium Ramat

abstract: The effects of nitrogen (N) supply on nitrogen metabolism in leaves of Chrysanthemum morifolium Ramat. were examined in five different stages throughout the growing season. The results suggested that flavonoids content was positively related to phenylalanine ammonia-lyase activity through the whole growing stage of the plant but with a decreasing correlation coefficient for increasing nitrogen supply. There was no correlation between flavonoids and 4-coumarate coenzyme A ligase. Soluble protein content was positively correlated with phenylalanine ammonia-lyase activity because there was little competition for the phenylalanine in the leaves under low nitrogen supply. Phenylalanine ammonia-lyase activity decreased gradually with increasing nitrogen supply because of the competition for the phenylalanine in protein synthesizes. The results suggest that nitrogen nutrition plays a key role in biosynthesis of enzymes in the leaves of C. morifolium.

text: Influence of nitrogen on the primary and secondary metabolism and synthesis of flavonoids in Chrysanthemum morifolium Ramat The effects of nitrogen (N) supply on nitrogen metabolism in leaves of Chrysanthemum morifolium Ramat. were examined in five different stages throughout the growing season. The results suggested that flavonoids content was positively related to phenylalanine ammonia-lyase activity through the whole growing stage of the plant but with a decreasing correlation coefficient for increasing nitrogen supply. There was no correlation between flavonoids and 4-coumarate coenzyme A ligase. Soluble protein content was positively correlated with phenylalanine ammonia-lyase activity because there was little competition for the phenylalanine in the leaves under low nitrogen supply. Phenylalanine ammonia-lyase activity decreased gradually with increasing nitrogen supply because of the competition for the phenylalanine in protein synthesizes. The results suggest that nitrogen nutrition plays a key role in biosynthesis of enzymes in the leaves of C. morifolium.

year: 2010

title: Development of a real-time PCR assay for the detection of the invasive clam, Corbula amurensis, in environmental samples

authors: Smith, Kirsty Fiona

subjects: environmental sampling

abstract: The detection of invasive species soon after an incursion, when the population is confined to a small area and at a low density, maximizes the probability of successful eradication. In response a number of sensitive molecular methods have been developed for identifying the larvae of marine invertebrate pests at extremely low concentrations. In this study we developed a highly sensitive real-time PCR assay targeting the 18S ribosomal DNA for the rapid and accurate identification of the Asian clam Corbula amurensis in environmental samples. Larvae of C. amurensis were spiked into commonly encountered sampling matrices including benthic assemblages, biofilms, sediment grabs and plankton net hauls, and the sensitivity of the assay was assessed. In this study the assay reliably detected one larva in up to 10 g of sediment, and five larvae in 10 g of benthic invertebrate and macro-algal assemblages. Seawater and benthic assemblage samples were collected from four major ports around New Zealand and all were negative for C. amurensis using the real-time PCR assay. This assay has the potential to enhance current surveillance methods, especially regarding morphologically difficult to identify early life-stages. Real-time PCR can be used with high through-put platforms and is extremely sensitive, increasing detection potential during initial stages of incursions.

text: Development of a real-time PCR assay for the detection of the invasive clam, Corbula amurensis, in environmental samples The detection of invasive species soon after an incursion, when the population is confined to a small area and at a low density, maximizes the probability of successful eradication. In response a number of sensitive molecular methods have been developed for identifying the larvae of marine invertebrate pests at extremely low concentrations. In this study we developed a highly sensitive real-time PCR assay targeting the 18S ribosomal DNA for the rapid and accurate identification of the Asian clam Corbula amurensis in environmental samples. Larvae of C. amurensis were spiked into commonly encountered sampling matrices including benthic assemblages, biofilms, sediment grabs and plankton net hauls, and the sensitivity of the assay was assessed. In this study the assay reliably detected one larva in up to 10 g of sediment, and five larvae in 10 g of benthic invertebrate and macro-algal assemblages. Seawater and benthic assemblage samples were collected from four major ports around New Zealand and all were negative for C. amurensis using the real-time PCR assay. This assay has the potential to enhance current surveillance methods, especially regarding morphologically difficult to identify early life-stages. Real-time PCR can be used with high through-put platforms and is extremely sensitive, increasing detection potential during initial stages of incursions.

year: 2011

title: How Maori have been affected through the notion and practice of schooling: A theoretical analysis

authors: Williamson, Laurie Thomas

abstract: Education has always been associated according to the public with a necessary function to equip individuals to fit into society when they reach adulthood. The fact that it functions with efficiency is attributed in part to the implementation of social norms, which "impose uniformity of behaviour" (Young 2006, p. 1).

This thesis delves into how Maori have been positioned under a mainstream schooling discourse which has put them at a disadvantage since the implementation of a discourse requires the subjugation of those who are not the majority power holders to assimilate into someone else's discourse. The effect of assimilation into a mainstream discourse has political implications for Maori, as schools are the gateway towards social stratification. This thesis will look at the historical and developmental concept of schooling within a western context, moving towards the development of formal schooling in New Zealand and also look at the sociological and political impact of schooling upon Maori students.

I will also differentiate between the terms schooling and education to suggest Maori forms of educational discourse has been marginalised in favour of assimilation into a supposedly equitable rigid, formal system of schooling. I will illustrate the broader concept of education that takes place outside of schools formal boundaries as a way to contrast the level of control and regulation the government initiates within the formal schooling environment. I will also look at the way schools have been used from its colonial beginnings in New Zealand as a way to civilise or impose one cultural practice on another as a way of breaking down uncivilised beliefs and traditions.

text: How Maori have been affected through the notion and practice of schooling: A theoretical analysis Education has always been associated according to the public with a necessary function to equip individuals to fit into society when they reach adulthood. The fact that it functions with efficiency is attributed in part to the implementation of social norms, which "impose uniformity of behaviour" (Young 2006, p. 1).

This thesis delves into how Maori have been positioned under a mainstream schooling discourse which has put them at a disadvantage since the implementation of a discourse requires the subjugation of those who are not the majority power holders to assimilate into someone else's discourse. The effect of assimilation into a mainstream discourse has political implications for Maori, as schools are the gateway towards social stratification. This thesis will look at the historical and developmental concept of schooling within a western context, moving towards the development of formal schooling in New Zealand and also look at the sociological and political impact of schooling upon Maori students.

I will also differentiate between the terms schooling and education to suggest Maori forms of educational discourse has been marginalised in favour of assimilation into a supposedly equitable rigid, formal system of schooling. I will illustrate the broader concept of education that takes place outside of schools formal boundaries as a way to contrast the level of control and regulation the government initiates within the formal schooling environment. I will also look at the way schools have been used from its colonial beginnings in New Zealand as a way to civilise or impose one cultural practice on another as a way of breaking down uncivilised beliefs and traditions.

year: 2012