title: Vascular functioning and development of the kiwifruit berry (Actinidia deliciosa)

authors: Morrison, Daniel

subjects: kiwifruit

abstract: The aim of this study was to understand kiwifruit berry development and the role of cell turgor and the phloem unloading pathway in development. Important aspects of berry development include the size of the fruit and its composition. The fresh weight growth curve of the kiwifruit berry was shown to be double sigmoid in shape. Dry weight accumulated linearly for the initial 139 days after anthesis (DAA). At this time the soluble solids concentration began to increase. Berry firmness was measured using two methods, with the penetrometer and with a new non-destructive method, utilising skinfold callipers. Both methods exhibited similar results, indicating that the skin callipers may be useful in the future for non-destructive berry rheological measurements.

Cell turgor was measured indirectly from measurements of symplasmic and apoplasmic solute potentials, and the matric potential of the berry. Apoplasmic sap, required to measure the apoplasmic solute potential, was extracted using two different methods the pressure chamber and through centrifugation. Measurements of sap osmotic potential suggest that the sap extracted using the centrifuge was contaminated with symplasmic sap, resulting in a negative cell turgor estimate. However, the pressure chamber technique provided apoplasmic sap that produced a more accurate estimate of cell turgor. Direct estimates of cell turgor were only obtained from the midpoint of the growing season because of contamination with symplasmic sap, but the values obtained were comparable to literature values for developing grape and tomato berries.

The phloem unloading pathway in the fruit was investigated using a symplasmic tracer dye, carboxyfluorescein diacetate coupled with 14C labelling and autoradiography. The phloem unloading pathway was symplasmic until 91 DAA when the dye was restricted to the phloem cells only, indicating a change to an apoplasmic pathway. However, due to the lack of functional unloading seen in radiolabelled samples, a change in the phloem unloading pathway could not be confirmed.

text: Vascular functioning and development of the kiwifruit berry (Actinidia deliciosa) The aim of this study was to understand kiwifruit berry development and the role of cell turgor and the phloem unloading pathway in development. Important aspects of berry development include the size of the fruit and its composition. The fresh weight growth curve of the kiwifruit berry was shown to be double sigmoid in shape. Dry weight accumulated linearly for the initial 139 days after anthesis (DAA). At this time the soluble solids concentration began to increase. Berry firmness was measured using two methods, with the penetrometer and with a new non-destructive method, utilising skinfold callipers. Both methods exhibited similar results, indicating that the skin callipers may be useful in the future for non-destructive berry rheological measurements.

Cell turgor was measured indirectly from measurements of symplasmic and apoplasmic solute potentials, and the matric potential of the berry. Apoplasmic sap, required to measure the apoplasmic solute potential, was extracted using two different methods the pressure chamber and through centrifugation. Measurements of sap osmotic potential suggest that the sap extracted using the centrifuge was contaminated with symplasmic sap, resulting in a negative cell turgor estimate. However, the pressure chamber technique provided apoplasmic sap that produced a more accurate estimate of cell turgor. Direct estimates of cell turgor were only obtained from the midpoint of the growing season because of contamination with symplasmic sap, but the values obtained were comparable to literature values for developing grape and tomato berries.

The phloem unloading pathway in the fruit was investigated using a symplasmic tracer dye, carboxyfluorescein diacetate coupled with 14C labelling and autoradiography. The phloem unloading pathway was symplasmic until 91 DAA when the dye was restricted to the phloem cells only, indicating a change to an apoplasmic pathway. However, due to the lack of functional unloading seen in radiolabelled samples, a change in the phloem unloading pathway could not be confirmed.

year: 2012

title: Cultural safety for LGBTQIA+ people: A narrative review and implications for health care in Malaysia

authors: Tan, Kyle K. H.

abstract: LGBTQIA+ people in Malaysia constitute a marginalised population as they are subjected to cisheterosexism that permeates every layer of society. Cisheterosexist ideologies in Malaysia find their eligibility on secular and religious laws that criminalise LGBTQIA+ identities, which have detrimental consequences on LGBTQIA+ people’s mental health and their ability to access equitable health care. Existing literature has revealed limitations for healthcare providers to employ a blinded approach (i.e., treat everyone the same) and practise culturally competency when seeing LGBTQIA+ patients. In this narrative review, we compiled international evidence of culturally safe care for LGBTQIA+ people and outlined its relevance to interrogating power relationships within healthcare practices and structures. Our reviewed findings brought together five components of culturally safe care for LGBTQIA+ people: power-enhancing care; inclusive healthcare institutions; continuous education and research; promotion of visibility; and individualised care. These components set crucial milestones for healthcare providers to reflect on ways to equalise power dynamics in a provider–patient relationship. The applicability and implication of culturally safe healthcare in Malaysia are succinctly discussed.

text: Cultural safety for LGBTQIA+ people: A narrative review and implications for health care in Malaysia LGBTQIA+ people in Malaysia constitute a marginalised population as they are subjected to cisheterosexism that permeates every layer of society. Cisheterosexist ideologies in Malaysia find their eligibility on secular and religious laws that criminalise LGBTQIA+ identities, which have detrimental consequences on LGBTQIA+ people’s mental health and their ability to access equitable health care. Existing literature has revealed limitations for healthcare providers to employ a blinded approach (i.e., treat everyone the same) and practise culturally competency when seeing LGBTQIA+ patients. In this narrative review, we compiled international evidence of culturally safe care for LGBTQIA+ people and outlined its relevance to interrogating power relationships within healthcare practices and structures. Our reviewed findings brought together five components of culturally safe care for LGBTQIA+ people: power-enhancing care; inclusive healthcare institutions; continuous education and research; promotion of visibility; and individualised care. These components set crucial milestones for healthcare providers to reflect on ways to equalise power dynamics in a provider–patient relationship. The applicability and implication of culturally safe healthcare in Malaysia are succinctly discussed.

year: 2022

title: Problem based learning and New Zealand legal education

authors: Mackinnon, Jacquelin

subjects: law

abstract: The literature (and e-literature) on Problem Based Learning in Law suggests that this approach has been adopted with enthusiasm by some lecturers in the United Kingdom, Europe and Hong Kong. This article will explore the adoption of PBL approaches through the literature in order to draw some conclusions about the nature of PBL approaches in law and their relationship with institutional approaches to legal education. Problem Based Learning approaches are not visible in New Zealand. The article discusses the reasons for PBL's invisibility and PBL's appropriateness in the New Zealand legal education context now and in the future of legal work. Part of the New Zealand context is the participation of the indigenous people in legal education and consideration is given to whether PBL can benefit Māori law students. 
It is suggested that PBL approaches to learning law promote: 
	Contextualisation
	Interdisciplinarity
	Integration of prior personal and/or professional knowledge
	Collaboration
	Enquiry skills
	Reflection and transition
	Self directed learning and self assessment
	Praxis.

Problem Based Learning requires and fosters reflexive participants, who are sufficiently conceptually literate to read and critique key aspects of the social order and to understand their own, and others' status and role in it. Reflexivity contributes to humanist as well as legal solutions to complex human problems. PBL approaches are consistent with legal education in an increasingly global employment market.

text: Problem based learning and New Zealand legal education The literature (and e-literature) on Problem Based Learning in Law suggests that this approach has been adopted with enthusiasm by some lecturers in the United Kingdom, Europe and Hong Kong. This article will explore the adoption of PBL approaches through the literature in order to draw some conclusions about the nature of PBL approaches in law and their relationship with institutional approaches to legal education. Problem Based Learning approaches are not visible in New Zealand. The article discusses the reasons for PBL's invisibility and PBL's appropriateness in the New Zealand legal education context now and in the future of legal work. Part of the New Zealand context is the participation of the indigenous people in legal education and consideration is given to whether PBL can benefit Māori law students. 
It is suggested that PBL approaches to learning law promote: 
	Contextualisation
	Interdisciplinarity
	Integration of prior personal and/or professional knowledge
	Collaboration
	Enquiry skills
	Reflection and transition
	Self directed learning and self assessment
	Praxis.

Problem Based Learning requires and fosters reflexive participants, who are sufficiently conceptually literate to read and critique key aspects of the social order and to understand their own, and others' status and role in it. Reflexivity contributes to humanist as well as legal solutions to complex human problems. PBL approaches are consistent with legal education in an increasingly global employment market.

year: 2006-06-01