title: Environmental and biogeographical drivers of the Leptospermum scoparium (mānuka) phyllosphere microbiome

authors: Noble, Anya Sophia

subjects: phyllosphere

abstract: A substantial body of research exists on the physiology and genetics of Leptospermum scoparium (mānuka); however, the mānuka phyllosphere has not yet been investigated. This research, therefore, provides the first exploration of the bacterial communities comprising the mānuka phyllosphere. In total, 89 samples were collected from five native mānuka forests during the November 2016 – January 2017 mānuka flowering season, and examined alongside spatial, environmental, and host-tree related metadata. Cultivation-independent methods, including DNA extraction and 16S rRNA gene PCR amplicon sequencing analysis, were used to characterise phyllosphere communities. Using a combination of statistical tests, the BLAST algorithm, and a PICRUSt analysis, a habitat-specific core microbiome comprising members of Acidobacteria, Alphaproteobacteria, Bacteroidetes, and Verrucomicrobia, was present in all samples. Distinct microbial biogeographical patterning was also identified in the mānuka phyllosphere whereby spatially structured gradients in environmental variables, such as temperature and precipitation, correlated with bacterial community structure.  The results from this study provide new insight pertaining to the structure, composition, and driving factors of the bacterial communities of the mānuka phyllosphere and shed light on theories of microbial ecology, such as microbial biogeography and core microbiomes. This research has the potential to open new avenues in both mānuka and microbial ecology fields of investigation within New Zealand.

text: Environmental and biogeographical drivers of the Leptospermum scoparium (mānuka) phyllosphere microbiome A substantial body of research exists on the physiology and genetics of Leptospermum scoparium (mānuka); however, the mānuka phyllosphere has not yet been investigated. This research, therefore, provides the first exploration of the bacterial communities comprising the mānuka phyllosphere. In total, 89 samples were collected from five native mānuka forests during the November 2016 – January 2017 mānuka flowering season, and examined alongside spatial, environmental, and host-tree related metadata. Cultivation-independent methods, including DNA extraction and 16S rRNA gene PCR amplicon sequencing analysis, were used to characterise phyllosphere communities. Using a combination of statistical tests, the BLAST algorithm, and a PICRUSt analysis, a habitat-specific core microbiome comprising members of Acidobacteria, Alphaproteobacteria, Bacteroidetes, and Verrucomicrobia, was present in all samples. Distinct microbial biogeographical patterning was also identified in the mānuka phyllosphere whereby spatially structured gradients in environmental variables, such as temperature and precipitation, correlated with bacterial community structure.  The results from this study provide new insight pertaining to the structure, composition, and driving factors of the bacterial communities of the mānuka phyllosphere and shed light on theories of microbial ecology, such as microbial biogeography and core microbiomes. This research has the potential to open new avenues in both mānuka and microbial ecology fields of investigation within New Zealand.

year: 2018

title: From sit-forward to lean-back: Using a mobile device to vary interactive pace

authors: Jones, Mark Hedley

subjects: computer science

abstract: Although online, handheld, mobile computers offer new possibilities in searching and retrieving information on the go, the fast-paced, "sit-forward" style of interaction may not be appropriate for all user search needs. In this paper, we explore how a handheld computer can be used to enable interactive search experiences that vary in pace from fast and immediate through to reflective and delayed. We describe a system that asynchronously combines an offline handheld computer and an online desktop Personal Computer, and discuss some results of an initial user evaluation.

text: From sit-forward to lean-back: Using a mobile device to vary interactive pace Although online, handheld, mobile computers offer new possibilities in searching and retrieving information on the go, the fast-paced, "sit-forward" style of interaction may not be appropriate for all user search needs. In this paper, we explore how a handheld computer can be used to enable interactive search experiences that vary in pace from fast and immediate through to reflective and delayed. We describe a system that asynchronously combines an offline handheld computer and an online desktop Personal Computer, and discuss some results of an initial user evaluation.

year: 2003-03

title: New Zealand regions, 1986 – 2001: Hospitalisation and some related health facts

authors: Pool, Ian

subjects: health

abstract: Once age and gender composition is controlled for, regional health differentials are a function of problems of health service delivery, of socio-economic variance, and overall Māori Pakeha health differences. They indicate relative levels of exclusion and of inequality. This paper shows that these differentials follow in general the patterns seen in other papers in this series.

text: New Zealand regions, 1986 – 2001: Hospitalisation and some related health facts Once age and gender composition is controlled for, regional health differentials are a function of problems of health service delivery, of socio-economic variance, and overall Māori Pakeha health differences. They indicate relative levels of exclusion and of inequality. This paper shows that these differentials follow in general the patterns seen in other papers in this series.

year: 2006-03