title: Lagunamides A and B: Cytotoxic and antimalarial Cyclodepsipeptides from the Marine cyanobacterium Lyngbya majuscule

authors: Tripathi, Ashootosh

subjects: chemistry

abstract: Lagunamides A (1) and B (2) are new cyclic depsipeptides isolated from the marine cyanobacterium Lyngbya majuscula obtained from Pulau Hantu Besar, Singapore. The planar structural characterization of these molecules was achieved by extensive spectroscopic analysis, including 2D NMR experiments. In addition to Marfey’s method and 3JH−H coupling constant values, a modified method based on Mosher’s reagents and analysis using LC-MS was deployed for the determination of the absolute configuration. Lagunamides A and B displayed significant antimalarial properties, with IC₅₀ values of 0.19 and 0.91 μM, respectively, when tested against Plasmodium falciparum. Lagunamides A and B also possessed potent cytotoxic activity against P388 murine leukemia cell lines, with IC₅₀  values of 6.4 and 20.5 nM, respectively. Furthermore, these cyanobacterial compounds exhibited moderate antiswarming activities when tested against Pseudomonas aeruginosa PA01.

text: Lagunamides A and B: Cytotoxic and antimalarial Cyclodepsipeptides from the Marine cyanobacterium Lyngbya majuscule Lagunamides A (1) and B (2) are new cyclic depsipeptides isolated from the marine cyanobacterium Lyngbya majuscula obtained from Pulau Hantu Besar, Singapore. The planar structural characterization of these molecules was achieved by extensive spectroscopic analysis, including 2D NMR experiments. In addition to Marfey’s method and 3JH−H coupling constant values, a modified method based on Mosher’s reagents and analysis using LC-MS was deployed for the determination of the absolute configuration. Lagunamides A and B displayed significant antimalarial properties, with IC₅₀ values of 0.19 and 0.91 μM, respectively, when tested against Plasmodium falciparum. Lagunamides A and B also possessed potent cytotoxic activity against P388 murine leukemia cell lines, with IC₅₀  values of 6.4 and 20.5 nM, respectively. Furthermore, these cyanobacterial compounds exhibited moderate antiswarming activities when tested against Pseudomonas aeruginosa PA01.

year: 2010

title: Food Preferences of the Brushtail Possum (Trichosurus vulpecula)

authors: Cameron, Kristie Elizabeth

subjects: food preferences

abstract: The common brushtail possum (Trichosurus vulpecula) has been reported to eat vegetation, fruit, invertebrates, and occasionally fungi, eggs and meat. The relative preference between food types found in the wild, however, has not been investigated systematically in a controlled laboratory study. This research investigated captive possums’ food choice using two different methods of preference assessment. The first experiment involved a single stimulus assessment of possums’ (n = 20) consumption of individually presented food items. More than 75% of possums consumed berries, locusts and mushrooms but fewer than 50% of possums consumed fivefinger, raw chicken and eggs. The second experiment that used a paired stimulus assessment to establish relative preference for those foods revealed that no single food was preferred by all possums. Overall locusts were the most preferred food, followed in order of preference by berries, egg, mushrooms, chicken and foliage. The single stimulus preference assessment confirmed the palatability of foods. The paired stimulus assessment provided a rank order of food preferences.

text: Food Preferences of the Brushtail Possum (Trichosurus vulpecula) The common brushtail possum (Trichosurus vulpecula) has been reported to eat vegetation, fruit, invertebrates, and occasionally fungi, eggs and meat. The relative preference between food types found in the wild, however, has not been investigated systematically in a controlled laboratory study. This research investigated captive possums’ food choice using two different methods of preference assessment. The first experiment involved a single stimulus assessment of possums’ (n = 20) consumption of individually presented food items. More than 75% of possums consumed berries, locusts and mushrooms but fewer than 50% of possums consumed fivefinger, raw chicken and eggs. The second experiment that used a paired stimulus assessment to establish relative preference for those foods revealed that no single food was preferred by all possums. Overall locusts were the most preferred food, followed in order of preference by berries, egg, mushrooms, chicken and foliage. The single stimulus preference assessment confirmed the palatability of foods. The paired stimulus assessment provided a rank order of food preferences.

year: 2013

title: Working at the interface: Indigenous students’ experience of undertaking doctoral studies in Aotearoa New Zealand

authors: McKinley, Elizabeth

subjects: Māori

abstract: Māori (indigenous)¹ doctoral students in Aotearoa New Zealand face challenges not usually experienced by other doctoral candidates. We draw on data from in-depth interviews with 38 Māori doctoral candidates and argue that because of the tensions between academic disciplinary knowledge frameworks and knowledge drawn from te ao Māori (the Māori world) indigenous students have additional cultural, academic, and personal demands placed on them while aiming to produce research theses that meet conventional standards of academic scholarship. Complex methodological and ethical issues also emerge in undertaking doctoral research projects situated at the interface of academy and indigenous communities. Moreover, Māori students experience various degrees of tension between their sometimes strong cultural identities and their emerging and, therefore, less certain identities as researchers and scholars.

text: Working at the interface: Indigenous students’ experience of undertaking doctoral studies in Aotearoa New Zealand Māori (indigenous)¹ doctoral students in Aotearoa New Zealand face challenges not usually experienced by other doctoral candidates. We draw on data from in-depth interviews with 38 Māori doctoral candidates and argue that because of the tensions between academic disciplinary knowledge frameworks and knowledge drawn from te ao Māori (the Māori world) indigenous students have additional cultural, academic, and personal demands placed on them while aiming to produce research theses that meet conventional standards of academic scholarship. Complex methodological and ethical issues also emerge in undertaking doctoral research projects situated at the interface of academy and indigenous communities. Moreover, Māori students experience various degrees of tension between their sometimes strong cultural identities and their emerging and, therefore, less certain identities as researchers and scholars.

year: 2011