title: Diversity of Methylobacterium Species Associated with New Zealand Native Plants

authors: Jahan, Rowshan

subjects: Methylobacterium

abstract: The genus Methylobacterium are pink-pigmented facultative methylotrophs (PPFMs), and are abundant colonizers of the phyllosphere, due to the availability of methanol, a waste product of pectin metabolism during plant cell division. Besides methanol cycling, Methylobacterium has important effects on plant health. The phyllosphere is an extreme environment with a landscape that is heterogeneous, continuously changing as the plant grows, and is exposed to very high ultra violet irradiation. Geographically, New Zealand has been isolated for over a million years, has a biologically diverse group of species, and is considered a biodiversity hotspot, with most of the native plants being endemic. We hypothesize that NZ native plants harbor diverse groups of Methylobacterium species, and to test this we aimed to isolate Methylobacterium species from the phyllosphere of native New Zealand plants. A leaf imprinting technique using methanol supplemented AMS agar media was used to isolate bacteria and diversity was determined using a combination of ARDRA, 16S rRNA gene sequencing and phylogenetic analysis. Methylobacterium spp. were successfully isolated from 19 of the 21 plant species used in this study. Eleven Methylobacterium species have been identified in association with the phyllosphere of native NZ plants: M. adhaesivum, M. brachiatum, M. komagatae, M. marchantiae, M. mesophilicum, M. phyllosphaerae, M. fujisawaense, M. oryzae, M. radiotolerans, M. tardum and M. zatmanii, with the first six being the most frequently isolated from more plant species. In this study other α, β, γ-proteobacterial species were also isolated: Hyphomicrobium, Methylopila, Rhizobium, Achromobacter, Methylophilus, Ramlibacter and Xanthomonas; Janibacter melonis (Actinomycetes); Niastella populi (Bacteroidetes) and Paenibacillus lautus (Firmicutes), highlighting the presence of potential novel methanol utilizer within the ecosystem. Results from this study indicate that Methylobacterium are abundant and dominant members of the NZ phyllosphere environment, with species diversity and composition dependent on the host plant species.

text: Diversity of Methylobacterium Species Associated with New Zealand Native Plants The genus Methylobacterium are pink-pigmented facultative methylotrophs (PPFMs), and are abundant colonizers of the phyllosphere, due to the availability of methanol, a waste product of pectin metabolism during plant cell division. Besides methanol cycling, Methylobacterium has important effects on plant health. The phyllosphere is an extreme environment with a landscape that is heterogeneous, continuously changing as the plant grows, and is exposed to very high ultra violet irradiation. Geographically, New Zealand has been isolated for over a million years, has a biologically diverse group of species, and is considered a biodiversity hotspot, with most of the native plants being endemic. We hypothesize that NZ native plants harbor diverse groups of Methylobacterium species, and to test this we aimed to isolate Methylobacterium species from the phyllosphere of native New Zealand plants. A leaf imprinting technique using methanol supplemented AMS agar media was used to isolate bacteria and diversity was determined using a combination of ARDRA, 16S rRNA gene sequencing and phylogenetic analysis. Methylobacterium spp. were successfully isolated from 19 of the 21 plant species used in this study. Eleven Methylobacterium species have been identified in association with the phyllosphere of native NZ plants: M. adhaesivum, M. brachiatum, M. komagatae, M. marchantiae, M. mesophilicum, M. phyllosphaerae, M. fujisawaense, M. oryzae, M. radiotolerans, M. tardum and M. zatmanii, with the first six being the most frequently isolated from more plant species. In this study other α, β, γ-proteobacterial species were also isolated: Hyphomicrobium, Methylopila, Rhizobium, Achromobacter, Methylophilus, Ramlibacter and Xanthomonas; Janibacter melonis (Actinomycetes); Niastella populi (Bacteroidetes) and Paenibacillus lautus (Firmicutes), highlighting the presence of potential novel methanol utilizer within the ecosystem. Results from this study indicate that Methylobacterium are abundant and dominant members of the NZ phyllosphere environment, with species diversity and composition dependent on the host plant species.

year: 2013

title: Covenons! We owe our store to the company’s soul...

authors: Yoos ii, Charles J.

subjects: business organization

abstract: We argue that in contemporary business organizations, in which fundamental purpose is construed to be increased value—especially in ‘participative’ organizations, in which non–hierarchal interaction (for example, work teams) is the norm; and in ‘adaptive’ organizations, in which unpredictable change is the rule—a process of values covenanting will be much more valueable than just espoused values or even values covenants. We propose such a process model for organizational values covenanting and argue that such covenanting reflects an anthropomorphism of the human character development process, validated in terms of the theory of viable systems.

text: Covenons! We owe our store to the company’s soul... We argue that in contemporary business organizations, in which fundamental purpose is construed to be increased value—especially in ‘participative’ organizations, in which non–hierarchal interaction (for example, work teams) is the norm; and in ‘adaptive’ organizations, in which unpredictable change is the rule—a process of values covenanting will be much more valueable than just espoused values or even values covenants. We propose such a process model for organizational values covenanting and argue that such covenanting reflects an anthropomorphism of the human character development process, validated in terms of the theory of viable systems.

year: 2008

title: Ngāti Whakaue Māngai Nui

authors: Hiini, Anaha Te Wheoro

subjects: Ngāti Whakaue Māngai Nui

abstract: Ko Ngāti Whakaue tētehi iwi ahurei o te waka o Te Arawa.  Ko tētehi taha ōna kei ngā tahataha o te kōroto moana o Te Rotorua-nui-a-Kahumatamomoe, i Waingaehe ki Waimihia.  Ko tētehi atu taha ōna kei te ūnga o te waka rongomaiwhiti o Te Arawa, arā ki Maketū.  
Kua whai mana a Ngāti Whakaue i ngā rautau e tū whakahīhī ai rātau hei iwi mana nui i ō rātau pūkenga me ō rātau pūmanawa kia mau tonu tō rātau Whakauetanga, tō rātau ahurea me ā rātau tikanga.   Ko tētehi pūkenga pēnei i te āheinga ki te whakairo i te kupu rōreka, ki te tuitui i te kōrero i whakaputaina ai te kōrero ‘Te Arawa Māngai Nui’.  He mea whakapuaki te kōrero rā e iwi kē ki te whakanui i ngā pūkōrero o ngā paepae o Te Arawa, ka mutu, koirā hoki tētehi pūkenga i hau ai te rongo o Ngāti Whakaue.  
Nā, ko te pūkenga e kōrerohia nei, ko te reo o Ngāti Whakaue.  Ko tōna oranga tonutanga te kaupapa matua o tēnei tuhinga e rangahaua ana i te putanga o te kōrero a tētehi o ngā koroua matatau o te iwi i te tau 1992, arā a Hamuera Mitchell, nāna tonu nei i whakapuaki ‘Ka mate te reo o Ngāti Whakaue ā te tekau tau’.  

Nō reira, he whakatewhatewhahanga tēnei tuhinga o te reo o Ngāti Whakaue o tua, o mohoa noa nei hoki kia mōhiotia ai te oranga, te matenga haeretanga rānei o te reo o te iwi, o ngā hapū, o te hapori, o te kāinga o nāianei, kia tau hoki mehemea āe rānei kua whai hua ngā rautaki reo o mua a Ngāti Whakaue ki te whakarauora i tōna ake reo, ā, ka aha te iwi e anga whakamua ai.  Ka mātaitia te āhua o te reo o Ngāti Whakaue kia mōhiotia mehemea i tika tā Hamera Mitchell i kī ai i te tau 1992, karekau rānei, mehemea rānei he wero kē nāna kia kaua e tukuna e te iwi o Ngāti Whakaue tō rātau reo kia mate.

Ko te tikanga rangahau Pākehā, ko te tikanga rangahau Māori, me ngā tikanga ako reo ka kaha mātaitia i tēnei tuhinga kia tika ai te kohinga o ngā raraunga o tēnei momo rangahau, ngā tikanga matatika a te wānanga, ngā tukanga tātari me te whakahōrapatanga atu i ngā whakataunga o te rangahau.  

Hei āpiti atu, ka kōrerohia ngā kaupapa mātauranga a Ngāti Whakaue, tae noa atu ki ngā tini kaupapa whakarauora reo a te iwi kua whakahaerehia mai i te tau 2005 ki ēnei rangi tonu nei mō te oranga o te reo o Ngāti Whakaue te take e anga tōtika atu ai ki te wetenga o te rautaki reo o mua a Ngāti Whakaue i whakahaerehia ai i ngā tau 2013 ki te tau 2017 i kitea ai ōna pikinga, ōna hekenga, ōna ngoikoretanga me ōna hua.  

Heoi anō, hei whaiwhai noa ake i te rautaki o mua nei, ko ngā mātātuhi kua mahue iho rā, ko te korenga o ngā arotakenga, ko te korenga o ngā pūrongo, ko te korenga o ētehi taunakitanga ā-tuhi nei, ko te korenga o tētehi aha kē atu rānei kia kitea he hua, he pikinga, he hekenga, he aha rānei.  Ahakoa tērā raru, nā te kitenga me te rongotanga o te pikinga haeretanga o te āhua o te reo, nā ngā kōrero ā-waha me ngā kauhau a ētehi mō ngā hua i puta ai i te rautaki reo o mua a Ngāti Whakaue i mana ai te whakahaumanutanga o te reo o ngā paepae o te iwi.

Nā te mea ko te reo Māori te remurere o te kaituhi nei, kua tekau mā waru tau ia e whai ana i te kaupapa i a ia e whakaako ana, i a ia e wānanga ana, i a ia hoki e whakapau kaha ana ki te whakaora anō i te reo Māori, kua whakaritea e ia tētehi rautaki reo hou e toru tekau tau te roa hei kōkiri mā te iwi e ora rawa atu anō ai te reo o Ngāti Whakaue, e whai hua anō ai hoki te whakapuakitanga o te kōrero rā ‘Te Arawa Māngai Nui’, hei tohu whakanui i ngā pūkenga kōrero o te iwi.

E ai ki te kōrero, ‘he tīmohea kua pakari’.  Nā roto i te āta rangahaua o te kaupapa nei i mōhio ai e ora tonu nei te reo o Ngāti Whakaue, heoi, kāore e pērā rawa te kaha o te oranga i te wā i a kui mā, i a koro mā.  Ahakoa te kōrero mō te matenga haeretanga o te reo o Ngāti Whakaue, nā ngā kaupapa whakaora reo a te iwi i ngā tau i piki anō ai te oranga o te reo, ka mutu, mā te whakahaerenga tonutanga o ngā wānanga nei e ora tonu anō ai te reo.

text: Ngāti Whakaue Māngai Nui Ko Ngāti Whakaue tētehi iwi ahurei o te waka o Te Arawa.  Ko tētehi taha ōna kei ngā tahataha o te kōroto moana o Te Rotorua-nui-a-Kahumatamomoe, i Waingaehe ki Waimihia.  Ko tētehi atu taha ōna kei te ūnga o te waka rongomaiwhiti o Te Arawa, arā ki Maketū.  
Kua whai mana a Ngāti Whakaue i ngā rautau e tū whakahīhī ai rātau hei iwi mana nui i ō rātau pūkenga me ō rātau pūmanawa kia mau tonu tō rātau Whakauetanga, tō rātau ahurea me ā rātau tikanga.   Ko tētehi pūkenga pēnei i te āheinga ki te whakairo i te kupu rōreka, ki te tuitui i te kōrero i whakaputaina ai te kōrero ‘Te Arawa Māngai Nui’.  He mea whakapuaki te kōrero rā e iwi kē ki te whakanui i ngā pūkōrero o ngā paepae o Te Arawa, ka mutu, koirā hoki tētehi pūkenga i hau ai te rongo o Ngāti Whakaue.  
Nā, ko te pūkenga e kōrerohia nei, ko te reo o Ngāti Whakaue.  Ko tōna oranga tonutanga te kaupapa matua o tēnei tuhinga e rangahaua ana i te putanga o te kōrero a tētehi o ngā koroua matatau o te iwi i te tau 1992, arā a Hamuera Mitchell, nāna tonu nei i whakapuaki ‘Ka mate te reo o Ngāti Whakaue ā te tekau tau’.  

Nō reira, he whakatewhatewhahanga tēnei tuhinga o te reo o Ngāti Whakaue o tua, o mohoa noa nei hoki kia mōhiotia ai te oranga, te matenga haeretanga rānei o te reo o te iwi, o ngā hapū, o te hapori, o te kāinga o nāianei, kia tau hoki mehemea āe rānei kua whai hua ngā rautaki reo o mua a Ngāti Whakaue ki te whakarauora i tōna ake reo, ā, ka aha te iwi e anga whakamua ai.  Ka mātaitia te āhua o te reo o Ngāti Whakaue kia mōhiotia mehemea i tika tā Hamera Mitchell i kī ai i te tau 1992, karekau rānei, mehemea rānei he wero kē nāna kia kaua e tukuna e te iwi o Ngāti Whakaue tō rātau reo kia mate.

Ko te tikanga rangahau Pākehā, ko te tikanga rangahau Māori, me ngā tikanga ako reo ka kaha mātaitia i tēnei tuhinga kia tika ai te kohinga o ngā raraunga o tēnei momo rangahau, ngā tikanga matatika a te wānanga, ngā tukanga tātari me te whakahōrapatanga atu i ngā whakataunga o te rangahau.  

Hei āpiti atu, ka kōrerohia ngā kaupapa mātauranga a Ngāti Whakaue, tae noa atu ki ngā tini kaupapa whakarauora reo a te iwi kua whakahaerehia mai i te tau 2005 ki ēnei rangi tonu nei mō te oranga o te reo o Ngāti Whakaue te take e anga tōtika atu ai ki te wetenga o te rautaki reo o mua a Ngāti Whakaue i whakahaerehia ai i ngā tau 2013 ki te tau 2017 i kitea ai ōna pikinga, ōna hekenga, ōna ngoikoretanga me ōna hua.  

Heoi anō, hei whaiwhai noa ake i te rautaki o mua nei, ko ngā mātātuhi kua mahue iho rā, ko te korenga o ngā arotakenga, ko te korenga o ngā pūrongo, ko te korenga o ētehi taunakitanga ā-tuhi nei, ko te korenga o tētehi aha kē atu rānei kia kitea he hua, he pikinga, he hekenga, he aha rānei.  Ahakoa tērā raru, nā te kitenga me te rongotanga o te pikinga haeretanga o te āhua o te reo, nā ngā kōrero ā-waha me ngā kauhau a ētehi mō ngā hua i puta ai i te rautaki reo o mua a Ngāti Whakaue i mana ai te whakahaumanutanga o te reo o ngā paepae o te iwi.

Nā te mea ko te reo Māori te remurere o te kaituhi nei, kua tekau mā waru tau ia e whai ana i te kaupapa i a ia e whakaako ana, i a ia e wānanga ana, i a ia hoki e whakapau kaha ana ki te whakaora anō i te reo Māori, kua whakaritea e ia tētehi rautaki reo hou e toru tekau tau te roa hei kōkiri mā te iwi e ora rawa atu anō ai te reo o Ngāti Whakaue, e whai hua anō ai hoki te whakapuakitanga o te kōrero rā ‘Te Arawa Māngai Nui’, hei tohu whakanui i ngā pūkenga kōrero o te iwi.

E ai ki te kōrero, ‘he tīmohea kua pakari’.  Nā roto i te āta rangahaua o te kaupapa nei i mōhio ai e ora tonu nei te reo o Ngāti Whakaue, heoi, kāore e pērā rawa te kaha o te oranga i te wā i a kui mā, i a koro mā.  Ahakoa te kōrero mō te matenga haeretanga o te reo o Ngāti Whakaue, nā ngā kaupapa whakaora reo a te iwi i ngā tau i piki anō ai te oranga o te reo, ka mutu, mā te whakahaerenga tonutanga o ngā wānanga nei e ora tonu anō ai te reo.

year: 2020