title: An eco-profile of thermoplastic protein derived from blood meal Part 1: allocation issues

authors: Bier, James Michael

subjects: Allocation

abstract: Purpose
A renewable thermoplastic called Novatein Thermoplastic Protein (NTP) has been developed from blood meal—a low-value by-product of the meat processing industry. The aim of this research was to develop a non-renewable energy and greenhouse gas emission eco-profile for cradle to gate production of NTP. Environmental impacts of supplying blood meal as a raw material were investigated using different allocation methods for farming and blood meal production. These included mass, economic, treating low-value by-products as waste and system expansion by substitution. In part 2, the entire system will be analysed on a cradle to gate basis and include the production of thermoplastic (NTP).
Methods
A theoretical NTP production facility was analysed for non-renewable primary energy use and greenhouse gas emissions. Data for feedstocks and process steps were obtained from published papers, government agency reports and engineering models. Mass and economic allocation models treating low-value by-products as waste and substitution were applied, and a sensitivity analysis was used to evaluate the impact of different methods of allocation on environmental impact.
Results and discussion
Non-renewable energy use in blood meal production varied between 5 (substitution) and 38 MJ (simple mass allocation) per kg of NTP. Greenhouse gas emissions varied between 0.4 (substitution), or even less if the biogenic carbon content is considered a credit, and 14 kg (mass allocation) CO2e per kg NTP.
Conclusions
It was concluded that both mass allocation and a waste assumption should be considered for the cradle to gate system. Mass allocation is common in other attributional studies and allows for a more transparent comparison. The most appropriate treatment of allocation in an attributional profile was to consider blood as a waste with regard to farming and meat processing, but include blood drying. This takes into account the motivations for farming and meat processing, but also recognises that there are other treatment options for blood that do not produce blood meal used in manufacturing NTP. This would allow NTP to be compared to other bioplastics as well as identifying hot spots in its cradle to gate production. It was also anticipated that results may be adapted in future cradle to grave assessments as product systems are developed.

text: An eco-profile of thermoplastic protein derived from blood meal Part 1: allocation issues Purpose
A renewable thermoplastic called Novatein Thermoplastic Protein (NTP) has been developed from blood meal—a low-value by-product of the meat processing industry. The aim of this research was to develop a non-renewable energy and greenhouse gas emission eco-profile for cradle to gate production of NTP. Environmental impacts of supplying blood meal as a raw material were investigated using different allocation methods for farming and blood meal production. These included mass, economic, treating low-value by-products as waste and system expansion by substitution. In part 2, the entire system will be analysed on a cradle to gate basis and include the production of thermoplastic (NTP).
Methods
A theoretical NTP production facility was analysed for non-renewable primary energy use and greenhouse gas emissions. Data for feedstocks and process steps were obtained from published papers, government agency reports and engineering models. Mass and economic allocation models treating low-value by-products as waste and substitution were applied, and a sensitivity analysis was used to evaluate the impact of different methods of allocation on environmental impact.
Results and discussion
Non-renewable energy use in blood meal production varied between 5 (substitution) and 38 MJ (simple mass allocation) per kg of NTP. Greenhouse gas emissions varied between 0.4 (substitution), or even less if the biogenic carbon content is considered a credit, and 14 kg (mass allocation) CO2e per kg NTP.
Conclusions
It was concluded that both mass allocation and a waste assumption should be considered for the cradle to gate system. Mass allocation is common in other attributional studies and allows for a more transparent comparison. The most appropriate treatment of allocation in an attributional profile was to consider blood as a waste with regard to farming and meat processing, but include blood drying. This takes into account the motivations for farming and meat processing, but also recognises that there are other treatment options for blood that do not produce blood meal used in manufacturing NTP. This would allow NTP to be compared to other bioplastics as well as identifying hot spots in its cradle to gate production. It was also anticipated that results may be adapted in future cradle to grave assessments as product systems are developed.

year: 2011

title: Preformulation and Formulation of Steroids and Assessment of an Electronically Modulated Intravaginal Device for Induced Calving or Oestrous synchronization of Cattle.

authors: Ismail, Ali Abdi

subjects: steroids

abstract: Drug delivery technology is currently advancing faster than at any time in biotechnology history. The challenge of drug delivery is to achieve a controlled release of therapeutic agents over an extended period. Controlled release potentially offers significant advantages over conventional dosage forms, by eliminating both under- and overdosing while maintaining a desired range of drug concentrations. An existing drug regimen for induced calving produces a declining blood profile that does not mimic the naturally occurring, gradually increasing, cortisol blood level around parturition effectively, causing animal health issues.  Likewise, while the existing progesterone controlled release systems for oestrous control successfully synchronise oestrus, it is however, associated with reduced fertility and as such other drugs have to be administered to improve the fertility during oestrus. Therefore, there is a need for a drug delivery system that is capable of delivering multiple drugs at various times and patterns. This research aimed to investigate, characterize, identify steroids with high absorption rates through vaginal mucosa and evaluate the potential of an electronic drug delivery system for the delivery of steroids for either the control of the bovine oestrous cycle or induced calving.
In order to identify steroids with high absorption rates across the vaginal mucosa, an in vitro permeation method was developed to screen selected steroids for their ability to permeate artificial and biological membranes. The steroids were pre-formulated to enhance their solubility and permeation through these membranes. Analytical UV and HPLC assays to characterise the pure and formulated steroidal compounds were also developed and validated. An assessment of an intravaginal Electronically Modulated Intravaginal Device (EMID) for the control of the bovine oestrous cycle or induced calving was carried out. Five different release assessment methods were investigated and critically evaluated in order to identify the most appropriate release assessment method for the EMID. These were: 1) the Drug Dissolution Test, 2) a weight loss method, 3) the dispensed weight method, 4) the determination of piston travel distance method, and 5) the rod expulsion from the EMID method. The methods investigated were critically evaluated in terms of ease of use and automation, reproducibility and cost/time savings. Optimisation of various components and construction materials of the EMID were also investigated. Animal trials were carried out using the original EMID (manufactured from polypropylene polymer) and modified inserts (manufactured from high density polyethylene polymer) to determine their retention rate in the animals. Accelerated stability testing of progesterone in suspension, oestradiol-17 tablets, cloprostenol as a powder blend and the driving mechanism of the EMID were examined.
The flux of the steroids was evaluated through poly-&-caprolactone and excised cow mucosa membranes using side-by-side permeation cells. 
Results indicated that progesterone followed by dexamethasone acetate and dexamethasone valerate showed higher permeability values through vaginal mucosa compared to dexamethasone or its other analogues. The weight loss method of the EMID proved to be an easy and appropriate method to measure the release rate from the EMID. A high density polyethylene polymer was identified as the most ideal body material for the insert compared to polypropylene body. Also double O-ring silicone, Elastollan WYO 1388-5 and solid silicone pistons were found to be amongst the best pistons tested and all performed well compared to other piston materials. There was a low retention rate with either the original EMID or the modified inserts. Further modification of retention wings of the EMID did not improve the retention rate, but a good blood profile response was obtained from cows treated with the complete EMID containing formulated progesterone. The formulations and driving mechanism were found to be stable under the tested conditions.  
Therefore, the EMID has potential for commercial application of induced calving or oestrous control per vaginum administration, reliant on improvement of its retention mechanism.

text: Preformulation and Formulation of Steroids and Assessment of an Electronically Modulated Intravaginal Device for Induced Calving or Oestrous synchronization of Cattle. Drug delivery technology is currently advancing faster than at any time in biotechnology history. The challenge of drug delivery is to achieve a controlled release of therapeutic agents over an extended period. Controlled release potentially offers significant advantages over conventional dosage forms, by eliminating both under- and overdosing while maintaining a desired range of drug concentrations. An existing drug regimen for induced calving produces a declining blood profile that does not mimic the naturally occurring, gradually increasing, cortisol blood level around parturition effectively, causing animal health issues.  Likewise, while the existing progesterone controlled release systems for oestrous control successfully synchronise oestrus, it is however, associated with reduced fertility and as such other drugs have to be administered to improve the fertility during oestrus. Therefore, there is a need for a drug delivery system that is capable of delivering multiple drugs at various times and patterns. This research aimed to investigate, characterize, identify steroids with high absorption rates through vaginal mucosa and evaluate the potential of an electronic drug delivery system for the delivery of steroids for either the control of the bovine oestrous cycle or induced calving.
In order to identify steroids with high absorption rates across the vaginal mucosa, an in vitro permeation method was developed to screen selected steroids for their ability to permeate artificial and biological membranes. The steroids were pre-formulated to enhance their solubility and permeation through these membranes. Analytical UV and HPLC assays to characterise the pure and formulated steroidal compounds were also developed and validated. An assessment of an intravaginal Electronically Modulated Intravaginal Device (EMID) for the control of the bovine oestrous cycle or induced calving was carried out. Five different release assessment methods were investigated and critically evaluated in order to identify the most appropriate release assessment method for the EMID. These were: 1) the Drug Dissolution Test, 2) a weight loss method, 3) the dispensed weight method, 4) the determination of piston travel distance method, and 5) the rod expulsion from the EMID method. The methods investigated were critically evaluated in terms of ease of use and automation, reproducibility and cost/time savings. Optimisation of various components and construction materials of the EMID were also investigated. Animal trials were carried out using the original EMID (manufactured from polypropylene polymer) and modified inserts (manufactured from high density polyethylene polymer) to determine their retention rate in the animals. Accelerated stability testing of progesterone in suspension, oestradiol-17 tablets, cloprostenol as a powder blend and the driving mechanism of the EMID were examined.
The flux of the steroids was evaluated through poly-&-caprolactone and excised cow mucosa membranes using side-by-side permeation cells. 
Results indicated that progesterone followed by dexamethasone acetate and dexamethasone valerate showed higher permeability values through vaginal mucosa compared to dexamethasone or its other analogues. The weight loss method of the EMID proved to be an easy and appropriate method to measure the release rate from the EMID. A high density polyethylene polymer was identified as the most ideal body material for the insert compared to polypropylene body. Also double O-ring silicone, Elastollan WYO 1388-5 and solid silicone pistons were found to be amongst the best pistons tested and all performed well compared to other piston materials. There was a low retention rate with either the original EMID or the modified inserts. Further modification of retention wings of the EMID did not improve the retention rate, but a good blood profile response was obtained from cows treated with the complete EMID containing formulated progesterone. The formulations and driving mechanism were found to be stable under the tested conditions.  
Therefore, the EMID has potential for commercial application of induced calving or oestrous control per vaginum administration, reliant on improvement of its retention mechanism.

year: 2007

title: Stemming the colonial environmental tide

authors: Joseph, Robert

subjects: human activities

abstract: Stemming the colonial environmental tide supports an Aotearoa New Zealand context that fundamentally acknowledges mātauranga and tikanga Māori law and shared co-governance and concurrent jurisdiction through 7 Aotearoa New Zealand EBM principles.

text: Stemming the colonial environmental tide Stemming the colonial environmental tide supports an Aotearoa New Zealand context that fundamentally acknowledges mātauranga and tikanga Māori law and shared co-governance and concurrent jurisdiction through 7 Aotearoa New Zealand EBM principles.

year: 2022-05