title: High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis

authors: Wu, Po-Hung

subjects: Escherichia coli

abstract: A fed-batch culture strategy for the production of recombinant Escherichia coli cells anchoring surface-displayed transglucosidase for use as a whole-cell biocatalyst for α-arbutin synthesis was developed. Lactose was used as an inducer of the recombinant protein. In fed-batch cultures, dissolved oxygen was used as the feed indicator for glucose, thus accumulation of glucose and acetate that affected the cell growth and recombinant protein production was avoided. Fed-batch fermentation with lactose induction yielded a biomass of 18 g/L, and the cells possessed very high transglucosylation activity. In the synthesis of α-arbutin by hydroquinone glucosylation, the whole-cell biocatalysts showed a specific activity of 501 nkat/g cell and produced 21 g/L of arbutin, which corresponded to 76% molar conversion. A sixfold increased productivity of whole cell biocatalysts was obtained in the fed-batch culture with lactose induction, as compared to batch culture induced by IPTG.

text: High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis A fed-batch culture strategy for the production of recombinant Escherichia coli cells anchoring surface-displayed transglucosidase for use as a whole-cell biocatalyst for α-arbutin synthesis was developed. Lactose was used as an inducer of the recombinant protein. In fed-batch cultures, dissolved oxygen was used as the feed indicator for glucose, thus accumulation of glucose and acetate that affected the cell growth and recombinant protein production was avoided. Fed-batch fermentation with lactose induction yielded a biomass of 18 g/L, and the cells possessed very high transglucosylation activity. In the synthesis of α-arbutin by hydroquinone glucosylation, the whole-cell biocatalysts showed a specific activity of 501 nkat/g cell and produced 21 g/L of arbutin, which corresponded to 76% molar conversion. A sixfold increased productivity of whole cell biocatalysts was obtained in the fed-batch culture with lactose induction, as compared to batch culture induced by IPTG.

year: 2008

title: Parkins v Parkins

authors: Chevalier-Watts, Juliet

abstract: This case came about by appeal from the District Court, which dismissed the appellant’s claim.  The appellant, Grant Parkins (Grant) declared 25% interest by way of institutional constructive trust over a property in the Marlborough Sounds (the property), which is opposed by his brothers, Steven Parkins (Steven) and Reece Parkins (Reece).   Grant states that his interest arises because of his substantial contributions to the property after it was bought by his late father, Morris Parkins (Morris).  The property was substantively developed after its purchase.  The property had been left in equal shares to the three brothers by Morris in his will. Of note is that the terms of Morris’ will were not challenged in any way by any of the brothers. Whilst this case does not challenge the law of institutional constructive trusts, it provides a useful and instructive summary of the established law and thus adds to the institutional constructive trust narrative.  Prior to considering the law, we will first provide an overview of the facts.

text: Parkins v Parkins This case came about by appeal from the District Court, which dismissed the appellant’s claim.  The appellant, Grant Parkins (Grant) declared 25% interest by way of institutional constructive trust over a property in the Marlborough Sounds (the property), which is opposed by his brothers, Steven Parkins (Steven) and Reece Parkins (Reece).   Grant states that his interest arises because of his substantial contributions to the property after it was bought by his late father, Morris Parkins (Morris).  The property was substantively developed after its purchase.  The property had been left in equal shares to the three brothers by Morris in his will. Of note is that the terms of Morris’ will were not challenged in any way by any of the brothers. Whilst this case does not challenge the law of institutional constructive trusts, it provides a useful and instructive summary of the established law and thus adds to the institutional constructive trust narrative.  Prior to considering the law, we will first provide an overview of the facts.

year: 2022-06-01

title: Reo Rua Pukapuka Pikitia: Whānau drawing on their community cultural wealth to create strategies for developing Te Reo Rangatira

authors: Brouwer, Jacqui

abstract: The use of Te Reo Māori in Early Childhood Education in Aotearoa New Zealand is mandated in the ECE curriculum. Research shows that picturebooks are powerful tools in language learning, but there is a lack of research concerning the use of bilingual picturebooks by whānau to support Māori language learning in a New Zealand kindergarten. This research presents findings from a 4-week programme in which five whānau members were introduced to several types of dual language picturebooks (Reo Rua Pukapuka Pikitia, RRPP) in a Puna Pukapuka Pikitia (PPP). Whānau explored RRPP and suggested how they could bring Reo Rangatira from RRPP into their homes to support language learning. Their suggestions aligned with Ellis’s principles of effective language teaching (Ellis, 2010) including the development of formulaic phrases, following the ways in which tamariki naturally learn language, encouraging opportunities for tamariki to speak and listen, as well as having interactions linked to RRPP.

text: Reo Rua Pukapuka Pikitia: Whānau drawing on their community cultural wealth to create strategies for developing Te Reo Rangatira The use of Te Reo Māori in Early Childhood Education in Aotearoa New Zealand is mandated in the ECE curriculum. Research shows that picturebooks are powerful tools in language learning, but there is a lack of research concerning the use of bilingual picturebooks by whānau to support Māori language learning in a New Zealand kindergarten. This research presents findings from a 4-week programme in which five whānau members were introduced to several types of dual language picturebooks (Reo Rua Pukapuka Pikitia, RRPP) in a Puna Pukapuka Pikitia (PPP). Whānau explored RRPP and suggested how they could bring Reo Rangatira from RRPP into their homes to support language learning. Their suggestions aligned with Ellis’s principles of effective language teaching (Ellis, 2010) including the development of formulaic phrases, following the ways in which tamariki naturally learn language, encouraging opportunities for tamariki to speak and listen, as well as having interactions linked to RRPP.

year: 2023