title: Solvent dependence of dynamic transitions in protein solutions

authors: Reat, Valerie

subjects: biology

abstract: A transition as a function of increasing temperature from harmonic to anharmonic dynamics has been observed in globular proteins by using spectroscopic, scattering, and computer simulation techniques. We present here results of a dynamic neutron scattering analysis of the solvent dependence of the picosecond-time scale dynamic transition behavior of solutions of a simple single-subunit enzyme, xylanase. The protein is examined in powder form, in D₂O, and in four two-component perdeuterated single-phase cryosolvents in which it is active and stable. The scattering profiles of the mixed solvent systems in the absence of protein are also determined. The general features of the dynamic transition behavior of the protein solutions follow those of the solvents. The dynamic transition in all of the mixed cryosolvent–protein systems is much more gradual than in pure D₂O, consistent with a distribution of energy barriers. The differences between the dynamic behaviors of the various cryosolvent protein solutions themselves are remarkably small. The results are consistent with a picture in which the picosecond-time scale atomic dynamics respond strongly to melting of pure water solvent but are relatively invariant in cryosolvents of differing compositions and melting points.

text: Solvent dependence of dynamic transitions in protein solutions A transition as a function of increasing temperature from harmonic to anharmonic dynamics has been observed in globular proteins by using spectroscopic, scattering, and computer simulation techniques. We present here results of a dynamic neutron scattering analysis of the solvent dependence of the picosecond-time scale dynamic transition behavior of solutions of a simple single-subunit enzyme, xylanase. The protein is examined in powder form, in D₂O, and in four two-component perdeuterated single-phase cryosolvents in which it is active and stable. The scattering profiles of the mixed solvent systems in the absence of protein are also determined. The general features of the dynamic transition behavior of the protein solutions follow those of the solvents. The dynamic transition in all of the mixed cryosolvent–protein systems is much more gradual than in pure D₂O, consistent with a distribution of energy barriers. The differences between the dynamic behaviors of the various cryosolvent protein solutions themselves are remarkably small. The results are consistent with a picture in which the picosecond-time scale atomic dynamics respond strongly to melting of pure water solvent but are relatively invariant in cryosolvents of differing compositions and melting points.

year: 2000

title: Review: Marine natural products

authors: Blunt, John W.

subjects: South China Sea

abstract: This review covers the literature published in 2011 for marine natural products, with 870 citations (558 for the period January to December 2011) referring to compounds isolated from marine microorganisms and phytoplankton, green, brown and red algae, sponges, cnidarians, bryozoans, molluscs, tunicates, echinoderms, mangroves and other intertidal plants and microorganisms. The emphasis is on new compounds (1152 for 2011), together with the relevant biological activities, source organisms and country of origin. Biosynthetic studies, first syntheses, and syntheses that lead to the revision of structures or stereochemistries, have been included.

text: Review: Marine natural products This review covers the literature published in 2011 for marine natural products, with 870 citations (558 for the period January to December 2011) referring to compounds isolated from marine microorganisms and phytoplankton, green, brown and red algae, sponges, cnidarians, bryozoans, molluscs, tunicates, echinoderms, mangroves and other intertidal plants and microorganisms. The emphasis is on new compounds (1152 for 2011), together with the relevant biological activities, source organisms and country of origin. Biosynthetic studies, first syntheses, and syntheses that lead to the revision of structures or stereochemistries, have been included.

year: 2013

title: ‘Let’s Get Wild’: Sensuous Geographies of Kāwhia Kai and Hokitika Wildfoods Festivals in Aōtearoa New Zealand

authors: Modlik, Melanie

subjects: Food Festivals

abstract: Food Festivals allow communities to celebrate local food and drink. This original thesis examines the mutual relationship between food festival organisers, festival attendees and festival spaces. Two unique New Zealand regional food festivals – Kāwhia Kai on the West Coast of the North Island and Wildfoods Hokitika on the West Coast of the South Island - are critically examined. The research addresses three questions. First, how do these food festivals construct their locations and vice versa? Second, in what ways do food festivals contribute to place identity? And, third, how do food festival attendees sensually experience the festivals?
The concept of embodiment provides the overarching framework for this research. With academic interests in both tourism and geography, I weave understandings of culinary tourism, festivals, tourist embodied experiences, sensuality, visercality and abjection to address my research questions. I use this theoretical ‘toolbox’ alongside qualitative methodological approaches which enable research participants to express their embodied experiences and emotions. Ten semi-structured interviews were conducted with festival organisers and food stall holders. Participant ‘sensing’ was carried out in both Kāwhia and Hokitika. In thinking about the social, cultural and ‘felt’ aspects of food, I also use my own body as an instrument of research. Promotional texts were collected and all data analysed using critical discourse analysis.
The empirical material is divided into two themes. The first theme focuses on location and place identity. Concepts of ‘place attachment’ and ‘sense of belonging’ construct food festival regions. The formation of ‘new colonial’ and ‘new Māori’ traditions is another way in which these food festivals created unique place identities. The second theme highlights the embodied experiences and spaces of both food festivals. Bodily senses, place and spaces are created through visceral and abject reactions to food experiences.
Food festivals have the potential to enhance the economic, social and cultural wellbeing of regional communities. The success of food festivals, however, is dependent on multi-sensual embodied experiences.

text: ‘Let’s Get Wild’: Sensuous Geographies of Kāwhia Kai and Hokitika Wildfoods Festivals in Aōtearoa New Zealand Food Festivals allow communities to celebrate local food and drink. This original thesis examines the mutual relationship between food festival organisers, festival attendees and festival spaces. Two unique New Zealand regional food festivals – Kāwhia Kai on the West Coast of the North Island and Wildfoods Hokitika on the West Coast of the South Island - are critically examined. The research addresses three questions. First, how do these food festivals construct their locations and vice versa? Second, in what ways do food festivals contribute to place identity? And, third, how do food festival attendees sensually experience the festivals?
The concept of embodiment provides the overarching framework for this research. With academic interests in both tourism and geography, I weave understandings of culinary tourism, festivals, tourist embodied experiences, sensuality, visercality and abjection to address my research questions. I use this theoretical ‘toolbox’ alongside qualitative methodological approaches which enable research participants to express their embodied experiences and emotions. Ten semi-structured interviews were conducted with festival organisers and food stall holders. Participant ‘sensing’ was carried out in both Kāwhia and Hokitika. In thinking about the social, cultural and ‘felt’ aspects of food, I also use my own body as an instrument of research. Promotional texts were collected and all data analysed using critical discourse analysis.
The empirical material is divided into two themes. The first theme focuses on location and place identity. Concepts of ‘place attachment’ and ‘sense of belonging’ construct food festival regions. The formation of ‘new colonial’ and ‘new Māori’ traditions is another way in which these food festivals created unique place identities. The second theme highlights the embodied experiences and spaces of both food festivals. Bodily senses, place and spaces are created through visceral and abject reactions to food experiences.
Food festivals have the potential to enhance the economic, social and cultural wellbeing of regional communities. The success of food festivals, however, is dependent on multi-sensual embodied experiences.

year: 2015