title: Investigating aquaphotomics for fruit quality assessment

authors: Kaur, Harpreet

subjects: Fruit quality

abstract: The methods of aquaphotomics were explored for internal fruit quality assessment, such as dry matter (DM) and soluble solid content (SSC) measurement using near infrared spectroscopy (NIRS). Fruits like apple and kiwifruit are more than 80% water. The NIR spectrum of whole intact fruit is dominated by water absorption peaks that shift and vary in shape with changes in quantities such as SSC, DM, and temperature. These variations can significantly reduce predictive model performance for fruit quality parameters.  Because the absorption peak at 1450 nm (first overtone of the OH stretch of water) and 970 nm (second overtone of the OH stretch of water) in the samples varies with SSC, DM concentration, and temperature, an investigation was undertaken on the aquaphotomics approach to quantify changes in the water structure that are apparent in the two wavelength regions.
The key question examined was whether aquaphotomics can help in the development of better and simpler prediction models or hardware for intact fruit quality measurements beneficial to the industry. To find answers to this question, several experiments were conducted. Much work in aquaphotomics has been done in the longer wavelength region, that is, at the first overtone (1300–1600 nm), which requires short pathlength cells due to higher water absorption. Experiments were performed on simple aqueous systems such as sucrose solution of various concentrations and an aqueous system such as apple and kiwifruit juice. The second overtone wavelength region (800‒1100 nm) was also scrutinized for fruit juice measurements using a long pathlength cell. Eventually, experiments were conducted on whole intact fruit.
A Fourier transform near infrared (FT-NIR) spectrophotometer was used to acquire spectra of aqueous samples over the wavelength range of 900 to 1800 nm. For whole, intact fruit spectra collection, spectrophotometers in the wavelength range below 1100 nm were utilized. An in-house benchtop spectrometer based on the Zeiss MMS-1 NIR spectrometer operated in interactance mode was used along with some commercially available handheld spectrophotometers such as the F-750 produce quality spectrometer and the SCiO spectrometer. The spectra acquired with the instruments were analyzed using multivariate data analysis. The calibration models were developed using partial least square regression (PLSR) and multiple linear regression to estimate quality attributes.
The NIR spectrum of fruit is affected by temperature. Therefore, a calibration equation developed at one fixed temperature cannot reliably predict samples at a different temperature. Using the framework of aquaphotomics, the changes within the water structure of fruit juice and whole intact fruit occurring with changes in temperature were analysed. Water wavelengths affected by temperature variation were identified in the first and second overtone regions. Using aquagrams, it appeared that different water species were activated in the first and second overtone region. The influence of increasing temperature on the peak absorbance of the juice was a lateral shift in the first overtone region, whereas it was vertical in the second overtone region of water. Two correction pre-treatments; extended multiplicative scatter correction (EMSC) and external parameter orthogonalization (EPO) were used in conjunction with principal component loading of water as an interferent spectrum. They were able to correct for temperature effects by at least a factor of 10 to insignificant levels.
It was concluded that the aquaphotomics paradigm offers fundamental insights into the role of the various light absorption mechanisms but only in conjunction with chemometrics tools, like EMSC and EPO, leading to an improvement in predictive model performance. The investigated methods have good potential to be used for quality assessment of fruit in industry.

text: Investigating aquaphotomics for fruit quality assessment The methods of aquaphotomics were explored for internal fruit quality assessment, such as dry matter (DM) and soluble solid content (SSC) measurement using near infrared spectroscopy (NIRS). Fruits like apple and kiwifruit are more than 80% water. The NIR spectrum of whole intact fruit is dominated by water absorption peaks that shift and vary in shape with changes in quantities such as SSC, DM, and temperature. These variations can significantly reduce predictive model performance for fruit quality parameters.  Because the absorption peak at 1450 nm (first overtone of the OH stretch of water) and 970 nm (second overtone of the OH stretch of water) in the samples varies with SSC, DM concentration, and temperature, an investigation was undertaken on the aquaphotomics approach to quantify changes in the water structure that are apparent in the two wavelength regions.
The key question examined was whether aquaphotomics can help in the development of better and simpler prediction models or hardware for intact fruit quality measurements beneficial to the industry. To find answers to this question, several experiments were conducted. Much work in aquaphotomics has been done in the longer wavelength region, that is, at the first overtone (1300–1600 nm), which requires short pathlength cells due to higher water absorption. Experiments were performed on simple aqueous systems such as sucrose solution of various concentrations and an aqueous system such as apple and kiwifruit juice. The second overtone wavelength region (800‒1100 nm) was also scrutinized for fruit juice measurements using a long pathlength cell. Eventually, experiments were conducted on whole intact fruit.
A Fourier transform near infrared (FT-NIR) spectrophotometer was used to acquire spectra of aqueous samples over the wavelength range of 900 to 1800 nm. For whole, intact fruit spectra collection, spectrophotometers in the wavelength range below 1100 nm were utilized. An in-house benchtop spectrometer based on the Zeiss MMS-1 NIR spectrometer operated in interactance mode was used along with some commercially available handheld spectrophotometers such as the F-750 produce quality spectrometer and the SCiO spectrometer. The spectra acquired with the instruments were analyzed using multivariate data analysis. The calibration models were developed using partial least square regression (PLSR) and multiple linear regression to estimate quality attributes.
The NIR spectrum of fruit is affected by temperature. Therefore, a calibration equation developed at one fixed temperature cannot reliably predict samples at a different temperature. Using the framework of aquaphotomics, the changes within the water structure of fruit juice and whole intact fruit occurring with changes in temperature were analysed. Water wavelengths affected by temperature variation were identified in the first and second overtone regions. Using aquagrams, it appeared that different water species were activated in the first and second overtone region. The influence of increasing temperature on the peak absorbance of the juice was a lateral shift in the first overtone region, whereas it was vertical in the second overtone region of water. Two correction pre-treatments; extended multiplicative scatter correction (EMSC) and external parameter orthogonalization (EPO) were used in conjunction with principal component loading of water as an interferent spectrum. They were able to correct for temperature effects by at least a factor of 10 to insignificant levels.
It was concluded that the aquaphotomics paradigm offers fundamental insights into the role of the various light absorption mechanisms but only in conjunction with chemometrics tools, like EMSC and EPO, leading to an improvement in predictive model performance. The investigated methods have good potential to be used for quality assessment of fruit in industry.

year: 2020

title: The effect of guided note taking during lectures on Thai university students’ understanding of electromagnetism

authors: Narjaikaew, Pattawan

subjects: guided note taking

abstract: This paper reports on the implementation of a guided note taking strategy to promote Thai students' understanding of electromagnetism during a lecture course. The aim of the study was to enhance student learning of electromagnetism concepts. The developed guided notes contain quotations, diagrams, pictures, problems, and blank spaces to encourage student interactive engagement with the lectures. The guided note templates were critiqued by a group of experienced university physics lecturers and piloted with graduate physics education students to check the content validity. Over 300 first year university students (aged about 18-19 years) attended lectures that did not involve guided note taking. Six hundred students participated in the guided note taking approach. Students' understanding of electromagnetism was investigated using a conceptual test. Comparison of the pre- and post-test results of the two groups of students indicated that students who were involved in the guided note taking approach performed better on the conceptual test than students who were not involved in this approach. From interviews, it was found that students viewed the guided note taking approach as a supportive tool that helped them concentrate on the lecture. Promoting student involvement in the lecture class through the process of guided note taking was shown to be a meaningful learning strategy for first year university physics classes.

text: The effect of guided note taking during lectures on Thai university students’ understanding of electromagnetism This paper reports on the implementation of a guided note taking strategy to promote Thai students' understanding of electromagnetism during a lecture course. The aim of the study was to enhance student learning of electromagnetism concepts. The developed guided notes contain quotations, diagrams, pictures, problems, and blank spaces to encourage student interactive engagement with the lectures. The guided note templates were critiqued by a group of experienced university physics lecturers and piloted with graduate physics education students to check the content validity. Over 300 first year university students (aged about 18-19 years) attended lectures that did not involve guided note taking. Six hundred students participated in the guided note taking approach. Students' understanding of electromagnetism was investigated using a conceptual test. Comparison of the pre- and post-test results of the two groups of students indicated that students who were involved in the guided note taking approach performed better on the conceptual test than students who were not involved in this approach. From interviews, it was found that students viewed the guided note taking approach as a supportive tool that helped them concentrate on the lecture. Promoting student involvement in the lecture class through the process of guided note taking was shown to be a meaningful learning strategy for first year university physics classes.

year: 2009

title: An integral rite of passage: embedding the aesthetic in adventure education in the pursuit of wellbeing

authors: Wagstaff, Ruth

subjects: wellbeing

abstract: The context for this study was the implementation of an arts integrated unit examined through the lenses of wellbeing, adventure education, children’s spirituality and aesthetic experience. The author’s personal experiences as a holistic educator, connecting theory and practice in educational settings, informed the devising of the learning experiences incorporated into this contemporary ‘Rites of Passage’, arts integrated unit. They also informed the qualitative methods selected for this research. Students reported on their own and others’ perceived state of “wholeness” and completeness of experience and the New Zealand Maori concepts of “hauora” – mental and emotional, physical, social and spiritual wellbeing were examined. Qualitative analysis of the data revealed that a holistic, integrative approach to learning was valued by the students and contributed significantly to their experience of wellbeing and wholeness. The coded data was considered in terms of a heuristic clustering of responses around the relational categories of Self, Others and the Environment. Connection, Relationship and Belonging emerged as the most important themes and, unexpectedly, Inclusion emerged as an important consideration to the participants in this study. Creative and resilience-building learning activities, as within this ‘Rites of Passage” unit and the integral “Wilderness Camp”, are supportive of the health and wellbeing of young learners. Such learning opportunities provide for intra-personal and inter-personal development, and contribute to their sense of self, place, meaning and purpose.

text: An integral rite of passage: embedding the aesthetic in adventure education in the pursuit of wellbeing The context for this study was the implementation of an arts integrated unit examined through the lenses of wellbeing, adventure education, children’s spirituality and aesthetic experience. The author’s personal experiences as a holistic educator, connecting theory and practice in educational settings, informed the devising of the learning experiences incorporated into this contemporary ‘Rites of Passage’, arts integrated unit. They also informed the qualitative methods selected for this research. Students reported on their own and others’ perceived state of “wholeness” and completeness of experience and the New Zealand Maori concepts of “hauora” – mental and emotional, physical, social and spiritual wellbeing were examined. Qualitative analysis of the data revealed that a holistic, integrative approach to learning was valued by the students and contributed significantly to their experience of wellbeing and wholeness. The coded data was considered in terms of a heuristic clustering of responses around the relational categories of Self, Others and the Environment. Connection, Relationship and Belonging emerged as the most important themes and, unexpectedly, Inclusion emerged as an important consideration to the participants in this study. Creative and resilience-building learning activities, as within this ‘Rites of Passage” unit and the integral “Wilderness Camp”, are supportive of the health and wellbeing of young learners. Such learning opportunities provide for intra-personal and inter-personal development, and contribute to their sense of self, place, meaning and purpose.

year: 2011