title: Switching toxin production on and off: intermittent microcystin synthesis in a Microcystis bloom

authors: Wood, Susanna A.

subjects: biology

abstract: Toxic cyanobacterial blooms are increasing in prevalence. Microcystins are the most commonly produced cyanotoxin. Despite extensive research the variables regulating microcystin production remain unclear. Using a RT-QPCR assay that allowed the precise measurement of mcyE transcriptional gene expression and an ELISA that enabled small changes in total microcystin concentrations to be monitored, we demonstrate for the first time that microcystin production is not always constitutive and that significant up- and downregulation in microcystin synthesis can occur on time scales of 2–6 h. Samples were collected over 3 days from a small eutrophic lake during a dense microcystin-producing Microcystis bloom. McyE gene transcripts were detected in only four out of 14 samples. Vicissitudes in both microcystin quotas and extracellular microcystin levels corresponded with changes in mcyE expression. During the period of exalted microcystin synthesis Microcystis sp. cell concentrations increased from 70 000 cells ml−1 to 4 000 000 cells ml−1. These data provide compelling evidence that changes in Microcystis cell concentrations influence microcystin production.

text: Switching toxin production on and off: intermittent microcystin synthesis in a Microcystis bloom Toxic cyanobacterial blooms are increasing in prevalence. Microcystins are the most commonly produced cyanotoxin. Despite extensive research the variables regulating microcystin production remain unclear. Using a RT-QPCR assay that allowed the precise measurement of mcyE transcriptional gene expression and an ELISA that enabled small changes in total microcystin concentrations to be monitored, we demonstrate for the first time that microcystin production is not always constitutive and that significant up- and downregulation in microcystin synthesis can occur on time scales of 2–6 h. Samples were collected over 3 days from a small eutrophic lake during a dense microcystin-producing Microcystis bloom. McyE gene transcripts were detected in only four out of 14 samples. Vicissitudes in both microcystin quotas and extracellular microcystin levels corresponded with changes in mcyE expression. During the period of exalted microcystin synthesis Microcystis sp. cell concentrations increased from 70 000 cells ml−1 to 4 000 000 cells ml−1. These data provide compelling evidence that changes in Microcystis cell concentrations influence microcystin production.

year: 2010

title: Exploring the front end of New Zealand curriculum in student teacher education: an example from language and mathematics education.

authors: Bailey, Judy

subjects: New Zealand curriculum

abstract: This paper reports on two components of a collaborative project conducted by members of the language and literacy education, mathematics education and social studies teaching teams at the Faculty of Education, The University of Waikato. The teams decided to research the implications of the front end of The New Zealand Curriculum document [NZC] (Ministry of Education, 2007). The front end of the document includes key competencies and a statement describing each learning area. The language and literacy team chose to explore student teacher understandings of the English essence statement and the way in which that learning area is structured. The mathematics education team explored student teacher understandings of and implications for the "thinking" key competency for the teaching and learning of mathematics. Data were collected through in-class observations and tasks, and the analysis of aspects of student assessment work. The findings highlighted the value of an explicit focus on a particular facet of the NZC along with the challenges student teachers experience in envisaging how this might play out in practice.

text: Exploring the front end of New Zealand curriculum in student teacher education: an example from language and mathematics education. This paper reports on two components of a collaborative project conducted by members of the language and literacy education, mathematics education and social studies teaching teams at the Faculty of Education, The University of Waikato. The teams decided to research the implications of the front end of The New Zealand Curriculum document [NZC] (Ministry of Education, 2007). The front end of the document includes key competencies and a statement describing each learning area. The language and literacy team chose to explore student teacher understandings of the English essence statement and the way in which that learning area is structured. The mathematics education team explored student teacher understandings of and implications for the "thinking" key competency for the teaching and learning of mathematics. Data were collected through in-class observations and tasks, and the analysis of aspects of student assessment work. The findings highlighted the value of an explicit focus on a particular facet of the NZC along with the challenges student teachers experience in envisaging how this might play out in practice.

year: 2010

title: Death and homelessness on the streets of Aotearoa New Zealand: Sustaining life

authors: Charvin-Fabre, Sandrine

abstract: In high-income countries, homeless people die 20 to 35 years earlier than domiciled populations. Despite this enduring reality, the issue of death inequality remains underexplored, especially in indigenous homeless contexts, facing the ongoing effects of colonisation. This study centres on the perspectives and aspirations of a Māori street homeless community, known as the “Peeps”. Its objective is twofold: to spotlight to unjust, untimely, and avoidable mortality impacting homeless communities in Aotearoa New Zealand, and to understand how life can be sustained against the adversity of street life. The thesis diverges from conventional public health approaches, which often dichotomise early deaths prevention through improved healthcare access during homelessness, and palliative care provision at the end of life of homeless people. This thesis explores “the temporal space of life prior to death”. Within that temporal realm, the omnipresent “risk of dying” that infiltrates the lives of homeless people, disrupting the linear progression toward death observed in domiciled populations, is recognised. This temporal space is understood as a zone of uncertainty and potentiality, wherein complex life-death dynamics operate, revealing broader political, societal and cultural tensions. This study encountered significant challenges, including the dearth of prior death-related research in Māori homeless contexts, and my non-Māori identity. In response, an indeterminate approach, rooted in relational ethics and complexity and systems thinking — aligned with Māori worldviews — was adopted.  

Part I of the thesis is conceptual and contextual. Part II invites the readers into the fragile “temporal space of life prior to death” and the cyclical research process. Cycle 1 focuses on understanding the local context of death inequality, through relational fieldwork and statistical analysis of coroners’ reports. This analysis uncovers a 30-year life expectancy disparity with domiciled populations, exacerbated by three-quarters of avoidable mortality, mainly due to chronic conditions and suicide. Subsequent cycles sustain relational fieldwork, using collaborative and critical performance ethnography. Cycle 2 reveals the Peeps’ resistance to access healthcare, rooted in experiences of transgenerational trauma and marginalisation of Māori beliefs and values in relation to death and dying within mainstream healthcare institutions. Collaboration with the Peeps leads to the co-creation of a drama, intended to initiate conversations with health professionals from the Peeps’ perspectives and aspirations, to improve the quality of care. This cycle also highlights the impact of disconnection processes with whānau (family, extended family) experienced by the Peeps. Progressing further, cycle 3 illuminates healing processes with whānau using Advance Care Planning, envisioned as a relational space, providing the Peeps with a sense of belonging, and spiritual continuity in the perspective of death and dying. 
 
In addition to shedding light on the profound death inequality that affect Māori homeless people, this thesis argues for homelessness to be recognised as “complex life-threatening conditions associated with serious-related health suffering”. Accordingly, the thesis advocates the shift toward comprehensive care, inclusive of a concurrent prevention and palliation approach over the course of homelessness, to mitigate its impact on the lives of Māori homeless people and enhance holistic wellbeing.

text: Death and homelessness on the streets of Aotearoa New Zealand: Sustaining life In high-income countries, homeless people die 20 to 35 years earlier than domiciled populations. Despite this enduring reality, the issue of death inequality remains underexplored, especially in indigenous homeless contexts, facing the ongoing effects of colonisation. This study centres on the perspectives and aspirations of a Māori street homeless community, known as the “Peeps”. Its objective is twofold: to spotlight to unjust, untimely, and avoidable mortality impacting homeless communities in Aotearoa New Zealand, and to understand how life can be sustained against the adversity of street life. The thesis diverges from conventional public health approaches, which often dichotomise early deaths prevention through improved healthcare access during homelessness, and palliative care provision at the end of life of homeless people. This thesis explores “the temporal space of life prior to death”. Within that temporal realm, the omnipresent “risk of dying” that infiltrates the lives of homeless people, disrupting the linear progression toward death observed in domiciled populations, is recognised. This temporal space is understood as a zone of uncertainty and potentiality, wherein complex life-death dynamics operate, revealing broader political, societal and cultural tensions. This study encountered significant challenges, including the dearth of prior death-related research in Māori homeless contexts, and my non-Māori identity. In response, an indeterminate approach, rooted in relational ethics and complexity and systems thinking — aligned with Māori worldviews — was adopted.  

Part I of the thesis is conceptual and contextual. Part II invites the readers into the fragile “temporal space of life prior to death” and the cyclical research process. Cycle 1 focuses on understanding the local context of death inequality, through relational fieldwork and statistical analysis of coroners’ reports. This analysis uncovers a 30-year life expectancy disparity with domiciled populations, exacerbated by three-quarters of avoidable mortality, mainly due to chronic conditions and suicide. Subsequent cycles sustain relational fieldwork, using collaborative and critical performance ethnography. Cycle 2 reveals the Peeps’ resistance to access healthcare, rooted in experiences of transgenerational trauma and marginalisation of Māori beliefs and values in relation to death and dying within mainstream healthcare institutions. Collaboration with the Peeps leads to the co-creation of a drama, intended to initiate conversations with health professionals from the Peeps’ perspectives and aspirations, to improve the quality of care. This cycle also highlights the impact of disconnection processes with whānau (family, extended family) experienced by the Peeps. Progressing further, cycle 3 illuminates healing processes with whānau using Advance Care Planning, envisioned as a relational space, providing the Peeps with a sense of belonging, and spiritual continuity in the perspective of death and dying. 
 
In addition to shedding light on the profound death inequality that affect Māori homeless people, this thesis argues for homelessness to be recognised as “complex life-threatening conditions associated with serious-related health suffering”. Accordingly, the thesis advocates the shift toward comprehensive care, inclusive of a concurrent prevention and palliation approach over the course of homelessness, to mitigate its impact on the lives of Māori homeless people and enhance holistic wellbeing.

year: 2024