title: Characterization of the potential drug target serine acetyltransferase (CysE) from Acinetobacter baumannii: A Pacific lens on biochemical research

authors: Usu, Jessicca Eva

abstract: Antimicrobial resistance (AMR) presents a critical global health threat, with Acinetobacter baumannii being a key pathogen due to its multidrug resistance. With no new antibiotic classes discovered since the 1980s, novel therapeutic targets are urgently needed. This thesis explores the potential of targeting the cysteine biosynthesis pathway, specifically the enzyme serine acetyltransferase (CysE), as a means to develop innovative antimicrobial strategies. Cysteine plays a pivotal role in bacterial survival and defense, particularly in oxidative stress resistance. Targeting its biosynthesis may impair bacterial virulence and enhance susceptibility to immune responses.

This research focuses on the characterization of CysE from A. baumannii, which uniquely expresses a short and full-length of this enzyme. While we were unable to optimize expression of the short form in this thesis, the long form was successfully expressed, purified, and biochemically and structurally characterized for the first time. Using X-ray crystallography, we present the three-dimensional structure of CysE from A. baumannii to a resolution of 2.14 Å. CysE adopts a homohexameric conformation, forming a dimer of trimers, and features an alpha-helical extension. SAXS analysis validated that the long form exists as a homohexamer in solution. Furthermore, using enzymatic assays we show CysE has serine acetyltransferase activity and is sensitive to feedback inhibition by pathway product L-cysteine, highlighting the regulatory mechanisms of this crucial enzyme. This work improves our understanding of cysteine biosynthesis in A. baumannii and provides a model for structure-based virtual inhibitor screening to identify potential new antimicrobials. 

In addition to its scientific objectives, this research is framed within the Fa'afaletui framework, an Indigenous Samoan methodology that ensures cultural perspectives are integrated into the scientific process. This approach ensures that Pacific values inform the direction of AMR research, fostering solutions that are not only scientifically rigorous but are also culturally relevant and accessible. The research serves to address the health inequities faced by Pacific peoples, particularly in relation to infectious diseases and AMR, and provides a foundation for future antimicrobial drug development efforts.

text: Characterization of the potential drug target serine acetyltransferase (CysE) from Acinetobacter baumannii: A Pacific lens on biochemical research Antimicrobial resistance (AMR) presents a critical global health threat, with Acinetobacter baumannii being a key pathogen due to its multidrug resistance. With no new antibiotic classes discovered since the 1980s, novel therapeutic targets are urgently needed. This thesis explores the potential of targeting the cysteine biosynthesis pathway, specifically the enzyme serine acetyltransferase (CysE), as a means to develop innovative antimicrobial strategies. Cysteine plays a pivotal role in bacterial survival and defense, particularly in oxidative stress resistance. Targeting its biosynthesis may impair bacterial virulence and enhance susceptibility to immune responses.

This research focuses on the characterization of CysE from A. baumannii, which uniquely expresses a short and full-length of this enzyme. While we were unable to optimize expression of the short form in this thesis, the long form was successfully expressed, purified, and biochemically and structurally characterized for the first time. Using X-ray crystallography, we present the three-dimensional structure of CysE from A. baumannii to a resolution of 2.14 Å. CysE adopts a homohexameric conformation, forming a dimer of trimers, and features an alpha-helical extension. SAXS analysis validated that the long form exists as a homohexamer in solution. Furthermore, using enzymatic assays we show CysE has serine acetyltransferase activity and is sensitive to feedback inhibition by pathway product L-cysteine, highlighting the regulatory mechanisms of this crucial enzyme. This work improves our understanding of cysteine biosynthesis in A. baumannii and provides a model for structure-based virtual inhibitor screening to identify potential new antimicrobials. 

In addition to its scientific objectives, this research is framed within the Fa'afaletui framework, an Indigenous Samoan methodology that ensures cultural perspectives are integrated into the scientific process. This approach ensures that Pacific values inform the direction of AMR research, fostering solutions that are not only scientifically rigorous but are also culturally relevant and accessible. The research serves to address the health inequities faced by Pacific peoples, particularly in relation to infectious diseases and AMR, and provides a foundation for future antimicrobial drug development efforts.

year: 2024

title: Audit education for future professionals: Perceptions of New Zealand auditors

authors: Chaffey, Jenna

subjects: audit education

abstract: Audit professionals are charged with gathering evidence and expressing their opinions on the financial claims made by others. In New Zealand, as elsewhere, these opinions are relied upon for a myriad of decisions as to the economic position and operations of an organisation. The educational experience that future professionals receive in their tertiary study is important because it lays the pedagogical foundation for this role. The aim of this study is to evaluate those teaching practices, subjects and techniques that might be of value to future audit professionals. The analysis is uniquely from the perspective of the professional members who are most likely to benefit from students' experiences. The literature is used to identify relevant questions, and a survey of 360 professional auditors in New Zealand yielded a 36.4% response rate. A triangulated analysis of comments and scaled questions reveals that these professionals are concerned with communication, small business engagements and the relationships between, for example, risk and planning and independence and ethics. Findings also confirm the value of experiential and practice-informed learning and direct us to a few topics that may accommodate non-traditional teaching methods. Conclusions consider implications for teaching and future research.

text: Audit education for future professionals: Perceptions of New Zealand auditors Audit professionals are charged with gathering evidence and expressing their opinions on the financial claims made by others. In New Zealand, as elsewhere, these opinions are relied upon for a myriad of decisions as to the economic position and operations of an organisation. The educational experience that future professionals receive in their tertiary study is important because it lays the pedagogical foundation for this role. The aim of this study is to evaluate those teaching practices, subjects and techniques that might be of value to future audit professionals. The analysis is uniquely from the perspective of the professional members who are most likely to benefit from students' experiences. The literature is used to identify relevant questions, and a survey of 360 professional auditors in New Zealand yielded a 36.4% response rate. A triangulated analysis of comments and scaled questions reveals that these professionals are concerned with communication, small business engagements and the relationships between, for example, risk and planning and independence and ethics. Findings also confirm the value of experiential and practice-informed learning and direct us to a few topics that may accommodate non-traditional teaching methods. Conclusions consider implications for teaching and future research.

year: 2011

title: Virtual hākari:  Sharing Māori culinary traditions in online communities

authors: Muru-Lanning, Charlotte

subjects: Kai Māori

abstract: For Māori, kai is a vital expression of identity, community, place and whakapapa. Traditionally, knowledge about kai is transmitted through customary community groupings and culturally embedded practices. However, with the rise of digital platforms, new spaces have emerged for Māori to gather and to share kai in virtual forms. 
This thesis observes how two Facebook groups – Kai Maori and KAI Basket NZ – operate as contemporary spaces for the transmission of Māori culinary knowledge and cultural identity. Kai Māori is considered alongside global studies of culinary traditions and understood through the notion of kai as whakapapa. The thesis addresses two core research questions: 1) How do Facebook groups contribute to an evolving concept of kai Māori? and 2) What kinds of relationships define these online kai communities? 
Guided by Māori data sovereignty (MDSov) principles, I observed interactions on both Facebook pages in two phases – one involving selective data collection over a twelve-month period from January to December 2024 the other involving systematic data collection over a four-week period from 10 December 2024 to 10 January 2025. The observation periods yielded 115 and 1293 posts respectively. A covert digital approach of ‘lurking’ was adopted as seeking prior consent may have affected the quality of the data and disrupted group dynamics. Lurking, as a method, sits uneasily alongside MDSov principles, particularly the principle of manaakitanga, requiring free, prior and informed consent. To address this I safeguarded the confidentiality of community members by seeking consent before reproducing content, removing group members’ names and excluding sensitive information. To analyse posts I used Ethnographic Content Analysis which allowed me to conduct a reflexive, iterative analysis of the posts in a way that was sensitive to the context in which the content was created.
These digital observations show that these groups serve as platforms for the transmission and innovation of kai Māori, encompassing edible components as well as the values enmeshed within these culinary traditions. Despite the mediated nature of these exchanges – where food is virtually consumed rather than physically shared, separate from traditional notions of community – these groups nurture meaningful connections among members, including those physically distant from their whenua. Through recipes, photos, stories, and commentary, the members of Kai Maori and KAI Basket NZ cultivate community identity and belonging via common cultural understandings, sharing of knowledge, a general spirit of sociality – and, importantly, a mutual love of kai.
I conceptualise these online spaces as virtual hākari, where the act of sharing virtual kai becomes an affirmation of identity and a form of cultural resistance. While online sharing may risk distancing kai practices and knowledge from traditional sites of transmission, the data suggests that these platforms may serve as conduits that encourage deeper cultural engagement offline. This thesis highlights the centrality of food in Māori cultural survival, sovereignty and resistance. In these digital spaces, kai Māori is not only celebrated but continually reimagined – reinforcing that to prepare, share and celebrate kai as Māori is to actively participate in the continuity of cultural identity.

text: Virtual hākari:  Sharing Māori culinary traditions in online communities For Māori, kai is a vital expression of identity, community, place and whakapapa. Traditionally, knowledge about kai is transmitted through customary community groupings and culturally embedded practices. However, with the rise of digital platforms, new spaces have emerged for Māori to gather and to share kai in virtual forms. 
This thesis observes how two Facebook groups – Kai Maori and KAI Basket NZ – operate as contemporary spaces for the transmission of Māori culinary knowledge and cultural identity. Kai Māori is considered alongside global studies of culinary traditions and understood through the notion of kai as whakapapa. The thesis addresses two core research questions: 1) How do Facebook groups contribute to an evolving concept of kai Māori? and 2) What kinds of relationships define these online kai communities? 
Guided by Māori data sovereignty (MDSov) principles, I observed interactions on both Facebook pages in two phases – one involving selective data collection over a twelve-month period from January to December 2024 the other involving systematic data collection over a four-week period from 10 December 2024 to 10 January 2025. The observation periods yielded 115 and 1293 posts respectively. A covert digital approach of ‘lurking’ was adopted as seeking prior consent may have affected the quality of the data and disrupted group dynamics. Lurking, as a method, sits uneasily alongside MDSov principles, particularly the principle of manaakitanga, requiring free, prior and informed consent. To address this I safeguarded the confidentiality of community members by seeking consent before reproducing content, removing group members’ names and excluding sensitive information. To analyse posts I used Ethnographic Content Analysis which allowed me to conduct a reflexive, iterative analysis of the posts in a way that was sensitive to the context in which the content was created.
These digital observations show that these groups serve as platforms for the transmission and innovation of kai Māori, encompassing edible components as well as the values enmeshed within these culinary traditions. Despite the mediated nature of these exchanges – where food is virtually consumed rather than physically shared, separate from traditional notions of community – these groups nurture meaningful connections among members, including those physically distant from their whenua. Through recipes, photos, stories, and commentary, the members of Kai Maori and KAI Basket NZ cultivate community identity and belonging via common cultural understandings, sharing of knowledge, a general spirit of sociality – and, importantly, a mutual love of kai.
I conceptualise these online spaces as virtual hākari, where the act of sharing virtual kai becomes an affirmation of identity and a form of cultural resistance. While online sharing may risk distancing kai practices and knowledge from traditional sites of transmission, the data suggests that these platforms may serve as conduits that encourage deeper cultural engagement offline. This thesis highlights the centrality of food in Māori cultural survival, sovereignty and resistance. In these digital spaces, kai Māori is not only celebrated but continually reimagined – reinforcing that to prepare, share and celebrate kai as Māori is to actively participate in the continuity of cultural identity.

year: 2025