title: Field and laboratory investigations of Echyridella menziesii (Unionida: Hyriidae) interactions with host fishes

authors: Hanrahan, Nicole Joy

subjects: freshwater mussel

abstract: Globally, the study of freshwater mussels has increased markedly in recent years, spurred on by recognition that many mussel populations are declining or are already extinct. The New Zealand freshwater mussel Echyridella menziesii (Unionida: Hyriidae) has a current threat classification of ‘At Risk, Declining’, in part reflecting disruption of recruitment on host fish. The life cycle of E. menziesii includes a parasitic stage in which the larvae (glochidia) must attach to a host fish to transform into the juvenile stage. However, no research has been conducted to understand whether fish develop immunity that limits infestation rates, and little is known about the spatial and temporal variations of mussel-host interactions in natural environments in New Zealand. This study therefore aimed to: i) determine whether multiple infestations of E. menziesii glochidia influenced the suitability of common bully (Gobiomorphus cotidianus) as a host; and ii) quantify fish host associations over the glochidial release period in three contrasting Waikato streams.

Common bully considered naïve to E. menziesii were infested with glochidia in the laboratory, and placed in individual, flow-through tanks (22oC) which were flushed every second day to measure glochidia detachment and juvenile excystment. Fishes were infested either one, two or three times, with ‘control’ fish infested for the first time in the second and third rounds. There were no differences in cumulative detachment rates of glochidia and no major reductions in metamorphosis success across multiple infestations. 

Metamorphosis success rate across all infestation rounds was ≤30%, and was highest when initial glochidia viability was also high. The lack of detectable immunity in common bully, at least after three sequential infestations, is promising for the future conservation of E. menziesii as host-fish are likely to be repetitively infested with glochidia over the mussel spawning season in a natural setting.

Infestation by glochidia was determined on the associated fish community, caught using a combination of electrofishing and minnow trapping. In parallel with these evaluations, mussel brood pouch development was assessed fortnightly from October 2018 to February 2019 at three Waikato stream sites. Peak glochidia release occurred in February when average monthly water temperatures were >18.8oC. A field method was developed to quantify glochidial attachment on external surfaces of fish, while internal attachment (gills, mouth) was quantified by laboratory dissection. While a range of fish hosts were identified, 86% of recorded glochidia were attached to Gobiomorphus species, including redfin bully (G. huttoni) and common/Crans bully (G. cotidianus/G. basalis). Most externally-attached glochidia (73%) occurred on caudal, pelvic and pectoral fins. Host associations appeared to favour benthic rather than pelagic species, and did not change throughout the mussel reproductive season despite seasonal change in fish communities.

This research demonstrates that common bully, and more broadly Gobiomorphus species, are important hosts for glochidia and do not develop immunity to repeated infestations. Gobiomorphus species have the potential to be very useful ‘tools’ to assist in restoring E. menziesii populations, and therefore conservation management must consider these host fish populations as well as other habitat and water quality factors affecting E. menziesii.

text: Field and laboratory investigations of Echyridella menziesii (Unionida: Hyriidae) interactions with host fishes Globally, the study of freshwater mussels has increased markedly in recent years, spurred on by recognition that many mussel populations are declining or are already extinct. The New Zealand freshwater mussel Echyridella menziesii (Unionida: Hyriidae) has a current threat classification of ‘At Risk, Declining’, in part reflecting disruption of recruitment on host fish. The life cycle of E. menziesii includes a parasitic stage in which the larvae (glochidia) must attach to a host fish to transform into the juvenile stage. However, no research has been conducted to understand whether fish develop immunity that limits infestation rates, and little is known about the spatial and temporal variations of mussel-host interactions in natural environments in New Zealand. This study therefore aimed to: i) determine whether multiple infestations of E. menziesii glochidia influenced the suitability of common bully (Gobiomorphus cotidianus) as a host; and ii) quantify fish host associations over the glochidial release period in three contrasting Waikato streams.

Common bully considered naïve to E. menziesii were infested with glochidia in the laboratory, and placed in individual, flow-through tanks (22oC) which were flushed every second day to measure glochidia detachment and juvenile excystment. Fishes were infested either one, two or three times, with ‘control’ fish infested for the first time in the second and third rounds. There were no differences in cumulative detachment rates of glochidia and no major reductions in metamorphosis success across multiple infestations. 

Metamorphosis success rate across all infestation rounds was ≤30%, and was highest when initial glochidia viability was also high. The lack of detectable immunity in common bully, at least after three sequential infestations, is promising for the future conservation of E. menziesii as host-fish are likely to be repetitively infested with glochidia over the mussel spawning season in a natural setting.

Infestation by glochidia was determined on the associated fish community, caught using a combination of electrofishing and minnow trapping. In parallel with these evaluations, mussel brood pouch development was assessed fortnightly from October 2018 to February 2019 at three Waikato stream sites. Peak glochidia release occurred in February when average monthly water temperatures were >18.8oC. A field method was developed to quantify glochidial attachment on external surfaces of fish, while internal attachment (gills, mouth) was quantified by laboratory dissection. While a range of fish hosts were identified, 86% of recorded glochidia were attached to Gobiomorphus species, including redfin bully (G. huttoni) and common/Crans bully (G. cotidianus/G. basalis). Most externally-attached glochidia (73%) occurred on caudal, pelvic and pectoral fins. Host associations appeared to favour benthic rather than pelagic species, and did not change throughout the mussel reproductive season despite seasonal change in fish communities.

This research demonstrates that common bully, and more broadly Gobiomorphus species, are important hosts for glochidia and do not develop immunity to repeated infestations. Gobiomorphus species have the potential to be very useful ‘tools’ to assist in restoring E. menziesii populations, and therefore conservation management must consider these host fish populations as well as other habitat and water quality factors affecting E. menziesii.

year: 2019

title: Application of a nutrition support protocol to encourage optimisation of nutrient intake in provincial academy rugby union athletes in New Zealand: Practical considerations and challenges from a team-based case study

authors: Roberts, Charlie J.

abstract: Provincial academies represent an important bridge between amateur and professional level rugby union in New Zealand. Athletes are provided with professional-level coaching; however, limited direct nutrition support is available. Congested training schedules and the requirement to work or study due to a lack of financial support may present a challenge towards athletes meeting nutrition requirements. The aim of the study was to facilitate improvement in nutrient intake, body composition and subjective well-being in provincial academy athletes via the implementation of a nutrition-support protocol based around behaviour change techniques. Significant increases in total energy (pre: 2492 ± 762 kcal; post: 2614 ± 625 kcal), relative energy (pre: 24.4 ± 7.5 kcal·kg; 25.5 ± 6.0 kcal·kg), total protein (pre: 131.1 ± 41.8 g; 153.8 ± 37.1 g) and relative protein (pre: 1.3 ± 0.4 g·kg; post: 1.5 ± 0.3 g·kg) were observed. Furthermore, changes in subjective sleep quality, stress, mood and upper body soreness were observed following the intervention. No changes were observed in body composition, carbohydrate or fat intake. Significant variability in nutrition and body composition changes highlights the importance of applying an individualised approach to nutrition support provision in developmental athletes. Practitioners working within these environments should be aware of the challenges and influences contributing to athletes’ nutrition choices and habits.

text: Application of a nutrition support protocol to encourage optimisation of nutrient intake in provincial academy rugby union athletes in New Zealand: Practical considerations and challenges from a team-based case study Provincial academies represent an important bridge between amateur and professional level rugby union in New Zealand. Athletes are provided with professional-level coaching; however, limited direct nutrition support is available. Congested training schedules and the requirement to work or study due to a lack of financial support may present a challenge towards athletes meeting nutrition requirements. The aim of the study was to facilitate improvement in nutrient intake, body composition and subjective well-being in provincial academy athletes via the implementation of a nutrition-support protocol based around behaviour change techniques. Significant increases in total energy (pre: 2492 ± 762 kcal; post: 2614 ± 625 kcal), relative energy (pre: 24.4 ± 7.5 kcal·kg; 25.5 ± 6.0 kcal·kg), total protein (pre: 131.1 ± 41.8 g; 153.8 ± 37.1 g) and relative protein (pre: 1.3 ± 0.4 g·kg; post: 1.5 ± 0.3 g·kg) were observed. Furthermore, changes in subjective sleep quality, stress, mood and upper body soreness were observed following the intervention. No changes were observed in body composition, carbohydrate or fat intake. Significant variability in nutrition and body composition changes highlights the importance of applying an individualised approach to nutrition support provision in developmental athletes. Practitioners working within these environments should be aware of the challenges and influences contributing to athletes’ nutrition choices and habits.

year: 2022-01-01

title: Boat electrofishing survey of fish abundance in the Ohau Channel, Rotorua, in 2017

authors: Hicks, Brendan J.

abstract: The aim of the survey was to provide on-going monitoring of the fish communities and abundance by boat electrofishing in the Ohau Channel, especially fish species that are taonga to Maori (tuna, or eels, morihana, or goldfish, and kōura, or freshwater crayfish). In the current study, we present the findings from the eleventh year of sampling (2017) and a summary of previous surveys.
We used the University of Waikato’s 4.5 m-long, aluminium-hulled electrofishing boat to catch a total of 1,110 fish and 3 kōura (18.2 kg in total) from 11 sites on 5 December 2017. The sites comprised 2,909 lineal m and 11,636 m2 in area. Kōura (freshwater crayfish) and 6 fish species were present, with common bully the most abundant species (up to 29.6 fish 100 m⁻² at the site 8, edge habitat). Goldfish (up to 14.2 100 m⁻²) was the next most abundant species, with most goldfish at sites 8 and 11 in and around the side channel. Common smelt were next the most abundant species (up to 11.4 fish 100 m⁻²). Mean density of bullies (7.8 fish 100 m⁻²) was much higher than for smelt (1.69 fish 100 m⁻²). Kōura had a patchy distribution with only 3 individuals were caught at two sites.
Comparing catches over the 11 years of sampling, the mean abundance of common bullies in 2017 was consistent with densities in most post-wall years (after 2007), but lower than in 2007 before wall closure (Newman-Keuls multiple range test, P = 0.034). The cause of fluctuating bully abundance is not known, and was not accounted for by changes in water clarity expressed as black disc distance (BDD), water temperature, or water conductivity. Poor water clarity can reduce the efficiency of electrofishing, but low BDD did not correspond with low common bully densities. In 2017, smelt abundance had recovered somewhat from the low catches from 2014 to 2016.
Goldfish biomass increased initially (2009-2010) because of targeted fishing in the side channel (site 11), which has dense macrophytes and offers good habitat for goldfish with no flow. However, the continued rise in goldfish density from 2012 on suggests a real increase in goldfish numbers. In contrast to most previous years, no eels were caught in 2017.
Analysis of fish densities before and after wall closure is hampered by the single data point before closure. However, we now have 10 years of post-wall data, and comparisons of means and standard deviations suggest that the number of bullies have been lower since 2008 with the exception of 2015, when bully densities were the same as 2007, i.e., before wall closure. The large number of small juvenile bullies (<30 mm) suggests that recruitment is taking place in the channel, so fluctuating bully abundance is likely to be controlled by factors other than wall closure.

text: Boat electrofishing survey of fish abundance in the Ohau Channel, Rotorua, in 2017 The aim of the survey was to provide on-going monitoring of the fish communities and abundance by boat electrofishing in the Ohau Channel, especially fish species that are taonga to Maori (tuna, or eels, morihana, or goldfish, and kōura, or freshwater crayfish). In the current study, we present the findings from the eleventh year of sampling (2017) and a summary of previous surveys.
We used the University of Waikato’s 4.5 m-long, aluminium-hulled electrofishing boat to catch a total of 1,110 fish and 3 kōura (18.2 kg in total) from 11 sites on 5 December 2017. The sites comprised 2,909 lineal m and 11,636 m2 in area. Kōura (freshwater crayfish) and 6 fish species were present, with common bully the most abundant species (up to 29.6 fish 100 m⁻² at the site 8, edge habitat). Goldfish (up to 14.2 100 m⁻²) was the next most abundant species, with most goldfish at sites 8 and 11 in and around the side channel. Common smelt were next the most abundant species (up to 11.4 fish 100 m⁻²). Mean density of bullies (7.8 fish 100 m⁻²) was much higher than for smelt (1.69 fish 100 m⁻²). Kōura had a patchy distribution with only 3 individuals were caught at two sites.
Comparing catches over the 11 years of sampling, the mean abundance of common bullies in 2017 was consistent with densities in most post-wall years (after 2007), but lower than in 2007 before wall closure (Newman-Keuls multiple range test, P = 0.034). The cause of fluctuating bully abundance is not known, and was not accounted for by changes in water clarity expressed as black disc distance (BDD), water temperature, or water conductivity. Poor water clarity can reduce the efficiency of electrofishing, but low BDD did not correspond with low common bully densities. In 2017, smelt abundance had recovered somewhat from the low catches from 2014 to 2016.
Goldfish biomass increased initially (2009-2010) because of targeted fishing in the side channel (site 11), which has dense macrophytes and offers good habitat for goldfish with no flow. However, the continued rise in goldfish density from 2012 on suggests a real increase in goldfish numbers. In contrast to most previous years, no eels were caught in 2017.
Analysis of fish densities before and after wall closure is hampered by the single data point before closure. However, we now have 10 years of post-wall data, and comparisons of means and standard deviations suggest that the number of bullies have been lower since 2008 with the exception of 2015, when bully densities were the same as 2007, i.e., before wall closure. The large number of small juvenile bullies (<30 mm) suggests that recruitment is taking place in the channel, so fluctuating bully abundance is likely to be controlled by factors other than wall closure.

year: 2018