title: The Purification of Cellulase and Hemicellulase Components from an Extreme Thermophile by the Cloning of Enzymes into E. coli

authors: Schofield, L.R.

subjects: biology

abstract: The use of heat treatment to purify enzymes by selective denaturation and then by the subsequent precipitation of denatured protein is a simple, rapid, and well established procedure. Successful applications are limited to those few enzymes that possess a thermostability considerably higher than the majority of cell proteins. The introduction of thermostable enzymes into the protein population of a mesophile by cloning offers a clear opportunity to employ a heat-treatment method of purification to its full advantage (e.g., see references 1 and 2).
In light of the difficulties involved in purifying bacterial cellulases, the cloning of some of the cellulase and hemicellulase genes of Caldocellum saccharolyticum into Escherichia coli has provided a welcome alternative procedure for obtaining pure enzymes.

text: The Purification of Cellulase and Hemicellulase Components from an Extreme Thermophile by the Cloning of Enzymes into E. coli The use of heat treatment to purify enzymes by selective denaturation and then by the subsequent precipitation of denatured protein is a simple, rapid, and well established procedure. Successful applications are limited to those few enzymes that possess a thermostability considerably higher than the majority of cell proteins. The introduction of thermostable enzymes into the protein population of a mesophile by cloning offers a clear opportunity to employ a heat-treatment method of purification to its full advantage (e.g., see references 1 and 2).
In light of the difficulties involved in purifying bacterial cellulases, the cloning of some of the cellulase and hemicellulase genes of Caldocellum saccharolyticum into Escherichia coli has provided a welcome alternative procedure for obtaining pure enzymes.

year: 1988

title: Using the theory of planned behaviour to predict intentions to reduce the environmental impact of travel

authors: Reddy, Siddhart

abstract: The use of private cars is responsible for a large amount of the world’s CO2 emissions
and the amount of pollution produced by the aviation industry is also growing at an alarming 
rate. Thus, it is important to understand the factors that can lead to pro-environmental 
behaviours (PEB), such as choosing more environmentally friendly options for air and car 
travel. I explored whether the Theory of Planned Behaviour (TPB) variables of
environmental attitudes, subjective norms, and perceived behavioural control (PBC) 
predicted individuals’ willingness to change their travel behaviour. A sample of 327 
participants from New Zealand and the United States of America completed an online 
questionnaire measuring TPB variables for low-, medium- and high-cost behaviours which 
were defined as short, medium, and long trips by both car and plane. I hypothesised that, in 
line with the A-B-C model, the TBP variables would be most predictive of intentions to 
reduce the environmental impact of medium-cost behaviours, rather than low- or high-cost 
behaviours. Attitudes, subjective norms, and PBC almost always significantly predicted
behavioural intention for the NZ sample, whilst only subjective norms and PBC were 
significant predictors for the USA sample, however, there was no support for the low-cost 
hypothesis or A-B-C model. Findings may influence and inform interventions and policy 
aimed at behavioural change in different contexts and that it may be beneficial for 
interventions to be tailored to specific populations based upon the strength of correlating 
variables.

text: Using the theory of planned behaviour to predict intentions to reduce the environmental impact of travel The use of private cars is responsible for a large amount of the world’s CO2 emissions
and the amount of pollution produced by the aviation industry is also growing at an alarming 
rate. Thus, it is important to understand the factors that can lead to pro-environmental 
behaviours (PEB), such as choosing more environmentally friendly options for air and car 
travel. I explored whether the Theory of Planned Behaviour (TPB) variables of
environmental attitudes, subjective norms, and perceived behavioural control (PBC) 
predicted individuals’ willingness to change their travel behaviour. A sample of 327 
participants from New Zealand and the United States of America completed an online 
questionnaire measuring TPB variables for low-, medium- and high-cost behaviours which 
were defined as short, medium, and long trips by both car and plane. I hypothesised that, in 
line with the A-B-C model, the TBP variables would be most predictive of intentions to 
reduce the environmental impact of medium-cost behaviours, rather than low- or high-cost 
behaviours. Attitudes, subjective norms, and PBC almost always significantly predicted
behavioural intention for the NZ sample, whilst only subjective norms and PBC were 
significant predictors for the USA sample, however, there was no support for the low-cost 
hypothesis or A-B-C model. Findings may influence and inform interventions and policy 
aimed at behavioural change in different contexts and that it may be beneficial for 
interventions to be tailored to specific populations based upon the strength of correlating 
variables.

year: 2024

title: National Maori and Pacific psychologies symposium: Claiming spaces

authors: Levy, Michelle Patricia

subjects: Maori

abstract: In 2002 the Maori and Psychology Research Unit made a valuable contribution to the  teaching and practice of psychology in Aotearoa via the two day Maori Graduates of Psychology Symposium: Making a Difference. Mid-way through 2006, our collective memories of the hard work in 2002  diminished, so much so that we once again considered hosting a similar event. Building on the foundations laid in 2002, the theme of the 2007 symposium was "Claiming Spaces", with our focus expanding to include peoples of the Pacific. This theme reflected that the time had come for Maori and Pacific Psychologies to move from the margins and claim legitimate space within psychology. It recognized that here in Aotearoa we have the potential to be pioneers in the development of psychologies relevant and applicable to Maori and Pacific peoples, and to better understand what science, culture and practice means when indigenous and cultural world views are prioritised. It was an inclusive theme, with the programme comprising presentations led by Maori and Pacific psychologists, graduates of psychology and psychology students. These presentations reflected on and made connections to the theme of "Claiming Spaces".

text: National Maori and Pacific psychologies symposium: Claiming spaces In 2002 the Maori and Psychology Research Unit made a valuable contribution to the  teaching and practice of psychology in Aotearoa via the two day Maori Graduates of Psychology Symposium: Making a Difference. Mid-way through 2006, our collective memories of the hard work in 2002  diminished, so much so that we once again considered hosting a similar event. Building on the foundations laid in 2002, the theme of the 2007 symposium was "Claiming Spaces", with our focus expanding to include peoples of the Pacific. This theme reflected that the time had come for Maori and Pacific Psychologies to move from the margins and claim legitimate space within psychology. It recognized that here in Aotearoa we have the potential to be pioneers in the development of psychologies relevant and applicable to Maori and Pacific peoples, and to better understand what science, culture and practice means when indigenous and cultural world views are prioritised. It was an inclusive theme, with the programme comprising presentations led by Maori and Pacific psychologists, graduates of psychology and psychology students. These presentations reflected on and made connections to the theme of "Claiming Spaces".

year: 2008