title: Identifying transition temperatures in bloodmeal-based thermoplastics using material pocket DMTA

authors: Bier, James Michael

subjects: Bioplastics

abstract: Bloodmeal can be used to manufacture thermoplastics, but requires water, urea, sodium sulphite, and sodium dodecyl sulphate to modify chain mobility. Transition temperatures of bloodmeal, modified bloodmeal, and processed bloodmeal-based thermoplastics were compared using material pocket dynamic mechanical thermal analysis. The glass transition temperature (T ɡ) of bloodmeal dropped from 493 to 263 K using only water as a plasticizer but was restored when freeze dried. Modifying bloodmeal lowered T ɡ to 193 K. This was raised by drying, but not to that of unmodified bloodmeal indicating a permanent change. Three additional transitions were identified above T ɡ, for modified bloodmeal between 300 and 480 K. These were thought to be transitions of dehydrated bulk amorphous regions, amorphous regions between crystallites and chains segments in crystallites and were also seen at lower temperatures when replacing some water with tri-ethylene glycol (TEG). Material pockets increased resolution in processed samples. One broad T ɡ was observed in consolidated bars, at 335 or 350 K with or without TEG. In material pockets, these resolved into three transitions, similar to those observed before processing. Changes in relative magnitudes suggested some chain rearrangement leading to more bulk amorphous regions. Differences were detected between onset of drop in storage modulus and peaks in loss modulus and tan δ in pockets or bars, but generally led to the same conclusions. For bar samples, it was helpful to compare natural and log modulus scales. Good practice would use all these techniques in parallel to correctly identify relaxation temperatures.

text: Identifying transition temperatures in bloodmeal-based thermoplastics using material pocket DMTA Bloodmeal can be used to manufacture thermoplastics, but requires water, urea, sodium sulphite, and sodium dodecyl sulphate to modify chain mobility. Transition temperatures of bloodmeal, modified bloodmeal, and processed bloodmeal-based thermoplastics were compared using material pocket dynamic mechanical thermal analysis. The glass transition temperature (T ɡ) of bloodmeal dropped from 493 to 263 K using only water as a plasticizer but was restored when freeze dried. Modifying bloodmeal lowered T ɡ to 193 K. This was raised by drying, but not to that of unmodified bloodmeal indicating a permanent change. Three additional transitions were identified above T ɡ, for modified bloodmeal between 300 and 480 K. These were thought to be transitions of dehydrated bulk amorphous regions, amorphous regions between crystallites and chains segments in crystallites and were also seen at lower temperatures when replacing some water with tri-ethylene glycol (TEG). Material pockets increased resolution in processed samples. One broad T ɡ was observed in consolidated bars, at 335 or 350 K with or without TEG. In material pockets, these resolved into three transitions, similar to those observed before processing. Changes in relative magnitudes suggested some chain rearrangement leading to more bulk amorphous regions. Differences were detected between onset of drop in storage modulus and peaks in loss modulus and tan δ in pockets or bars, but generally led to the same conclusions. For bar samples, it was helpful to compare natural and log modulus scales. Good practice would use all these techniques in parallel to correctly identify relaxation temperatures.

year: 2012

title: Different kinds of argument for applying property law to resource consents

authors: Barton, Barry

abstract: Two recent Court of Appeal cases present an opportunity for making progress in unravelling some of the puzzles about the extent to which the holder of a Resource Management Act resource consent, such as a water permit, holds property rights. These problems are not unique to the RMA or to New Zealand, and everywhere they involve a difficult interweaving of private law and public law. There is no generally accepted body of law for ascertaining whether the attributes of property ownership attach to permits granted under statutes: B Barton, “Property Rights Created under Statute in Common Law Legal Systems” in A McHarg and others (eds) Property and the Law in Energy and Natural Resources (Oxford University Press, Oxford, 2010) 80–99. But a useful distinction can be drawn between two different purposes to which property arguments can be put, and it provides a basis on which to reconcile two recent cases, Hampton v Canterbury Regional Council (Environment Canterbury) [2015] NZCA 509, and Greenshell New Zealand Ltd (in rec) v Kennedy Bay Mussel Co (NZ) Ltd [2015] NZCA 374, [2016] 2 NZLR 44.

text: Different kinds of argument for applying property law to resource consents Two recent Court of Appeal cases present an opportunity for making progress in unravelling some of the puzzles about the extent to which the holder of a Resource Management Act resource consent, such as a water permit, holds property rights. These problems are not unique to the RMA or to New Zealand, and everywhere they involve a difficult interweaving of private law and public law. There is no generally accepted body of law for ascertaining whether the attributes of property ownership attach to permits granted under statutes: B Barton, “Property Rights Created under Statute in Common Law Legal Systems” in A McHarg and others (eds) Property and the Law in Energy and Natural Resources (Oxford University Press, Oxford, 2010) 80–99. But a useful distinction can be drawn between two different purposes to which property arguments can be put, and it provides a basis on which to reconcile two recent cases, Hampton v Canterbury Regional Council (Environment Canterbury) [2015] NZCA 509, and Greenshell New Zealand Ltd (in rec) v Kennedy Bay Mussel Co (NZ) Ltd [2015] NZCA 374, [2016] 2 NZLR 44.

year: 2016

title: Contrasting microbial communities across anthropogenic pollution gradients: MV Rena shipwreck versus urban pressures

authors: Reihana, Kiri

subjects: microbial

abstract: With the catastrophic environmental event that was the MV Rena ship wreck and oil spill in the Bay of Plenty area in 2011, a serendipitous opportunity to laterally explore a variety of impacts in the marine biosphere emerged. One of those opportunities is studied here; how microbial communities respond to stress in context of existing ambient local anthropogenic influences. The locational backdrop to this study is also relevant and provides an additional element of complexity as the Bay of Plenty region is located in the middle of the Taupo Volcanic Zone, hence microbial communities are likely to reflect the regional geology. Therefore a research project was possible where a pollutant gradient was selected based on a range of locations reflecting different elements of natural versus human long term and acute impact stresses. The concept was to establish a platform of preliminary study from which more detailed could follow. In addition this study aimed at providing a link between microbial environmental responses to stress in a context that could permit uptake of scientific evidence for cultural impact assessments given the strong cultural focus on MV Rena impacts by government and the public.
A significant element of the exploratory process was to identify methodologies which may be of use in ascribing microbial community composition with the microenvironment in a manner that allows comparison with research reported in the literature.
Review of all results showed that sites from widely varying geologies and environmental/stress condition were more similar than was initially predicted. This is in sharp contrast to expectations generated from the literature. 
At the sites of Astrolabe Reef (Otaiti) (impacted by the ship wreck, major oil spill and heavy metal pollution event) and Maketu (impacted by a eutrophic estuary), similar consortia transpired, despite strongly different sources of stress, substantiating the microbial biogeography theory: ‘Everything is everywhere’ and ‘the environment selects’. However at Tauranga Harbour long term human activity appears to have had a significant ‘additional’ effect on microbial communities. Our challenge has hence become one to separate out natural versus a range of anthropogenic stressors. 
Underlying the general overall trends, subtle community effects such as at Astrolabe Reef, which supported significantly different dominant communities to the other two sites. It was expected that Tauranga Harbour and Maketu Estuary (adjacent coastally), would be more similar. Of all the sites one at the impacted Maketu, site 1 (M1) and the impacted Tauranga site 2 (T2), were highly similar at 96% from statistical analysis suggesting, the original assumption of visually similar site characteristics (impacted sites with low residence time and thick algal matts) do support similar microbial communities. Substantiating the theory that ‘everything is everywhere’ at these two sites, even though the variables and elemental composition were dissimilar. 
Rare and abundant species were separated out and analysed to elucidate patterns or trends in the communities. Overall the abundant species clustered, into impacted versus non impacted sites, for the rare species Tauranga and Maketu clustered clearly following the same trend; while the Astrolabe reef sites clustered together. The clear differentiation in the rare species from the Astrolabe reef sites, suggests that those species may make up the endemic bacterial communities at Astrolabe Reef with additional effects of Rena pollution suggested at some of the sites close to the wreck.
A general discussion of the cultural and economic impacts of the Rena oil spill, gave context to the holistic human impacts sustained by the local Māori, specifically the disconnect of their precious livelihoods and unique relationship with the environment. These holistic concepts could compromise a thesis in themselves so in this report their definitions are by no means finite.     
This study attempted to interrelate both cultural, ecological biology and microbiology science components for the purpose of a more comprehensive investigation of microbial responses to the Rena oil spill and contrast these to local anthropogenic pressures. However being the first attempt to catalogue the previously unknown microbial communities in the Bay of Plenty area, meant that sample regimes, techniques, processing and analysis were all experimental. Despite this, effects attributable to the Rena were identified that lay beyond the large scale variability identified for Bay of Plenty microbial community assemblages. 
These research results provide an important primary contribution to microbial understandings in the New Zealand context, and as a baseline mapping exercise for the Bay of Plenty region.

text: Contrasting microbial communities across anthropogenic pollution gradients: MV Rena shipwreck versus urban pressures With the catastrophic environmental event that was the MV Rena ship wreck and oil spill in the Bay of Plenty area in 2011, a serendipitous opportunity to laterally explore a variety of impacts in the marine biosphere emerged. One of those opportunities is studied here; how microbial communities respond to stress in context of existing ambient local anthropogenic influences. The locational backdrop to this study is also relevant and provides an additional element of complexity as the Bay of Plenty region is located in the middle of the Taupo Volcanic Zone, hence microbial communities are likely to reflect the regional geology. Therefore a research project was possible where a pollutant gradient was selected based on a range of locations reflecting different elements of natural versus human long term and acute impact stresses. The concept was to establish a platform of preliminary study from which more detailed could follow. In addition this study aimed at providing a link between microbial environmental responses to stress in a context that could permit uptake of scientific evidence for cultural impact assessments given the strong cultural focus on MV Rena impacts by government and the public.
A significant element of the exploratory process was to identify methodologies which may be of use in ascribing microbial community composition with the microenvironment in a manner that allows comparison with research reported in the literature.
Review of all results showed that sites from widely varying geologies and environmental/stress condition were more similar than was initially predicted. This is in sharp contrast to expectations generated from the literature. 
At the sites of Astrolabe Reef (Otaiti) (impacted by the ship wreck, major oil spill and heavy metal pollution event) and Maketu (impacted by a eutrophic estuary), similar consortia transpired, despite strongly different sources of stress, substantiating the microbial biogeography theory: ‘Everything is everywhere’ and ‘the environment selects’. However at Tauranga Harbour long term human activity appears to have had a significant ‘additional’ effect on microbial communities. Our challenge has hence become one to separate out natural versus a range of anthropogenic stressors. 
Underlying the general overall trends, subtle community effects such as at Astrolabe Reef, which supported significantly different dominant communities to the other two sites. It was expected that Tauranga Harbour and Maketu Estuary (adjacent coastally), would be more similar. Of all the sites one at the impacted Maketu, site 1 (M1) and the impacted Tauranga site 2 (T2), were highly similar at 96% from statistical analysis suggesting, the original assumption of visually similar site characteristics (impacted sites with low residence time and thick algal matts) do support similar microbial communities. Substantiating the theory that ‘everything is everywhere’ at these two sites, even though the variables and elemental composition were dissimilar. 
Rare and abundant species were separated out and analysed to elucidate patterns or trends in the communities. Overall the abundant species clustered, into impacted versus non impacted sites, for the rare species Tauranga and Maketu clustered clearly following the same trend; while the Astrolabe reef sites clustered together. The clear differentiation in the rare species from the Astrolabe reef sites, suggests that those species may make up the endemic bacterial communities at Astrolabe Reef with additional effects of Rena pollution suggested at some of the sites close to the wreck.
A general discussion of the cultural and economic impacts of the Rena oil spill, gave context to the holistic human impacts sustained by the local Māori, specifically the disconnect of their precious livelihoods and unique relationship with the environment. These holistic concepts could compromise a thesis in themselves so in this report their definitions are by no means finite.     
This study attempted to interrelate both cultural, ecological biology and microbiology science components for the purpose of a more comprehensive investigation of microbial responses to the Rena oil spill and contrast these to local anthropogenic pressures. However being the first attempt to catalogue the previously unknown microbial communities in the Bay of Plenty area, meant that sample regimes, techniques, processing and analysis were all experimental. Despite this, effects attributable to the Rena were identified that lay beyond the large scale variability identified for Bay of Plenty microbial community assemblages. 
These research results provide an important primary contribution to microbial understandings in the New Zealand context, and as a baseline mapping exercise for the Bay of Plenty region.

year: 2016