title: Digital trust - trusted computing and beyond: A position paper

authors: Akram, Raja Naeem

subjects: Cloud computing

abstract: Along with the invention of computers and interconnected networks, physical societal notions like security, trust, and privacy entered the digital environment. The concept of digital environments begins with the trust (established in the real world) in the organisation/individual that manages the digital resources. This concept evolved to deal with the rapid growth of the Internet, where it became impractical for entities to have prior offline (real world) trust. The evolution of digital trust took diverse approaches and now trust is defined and understood differently across heterogeneous domains. This paper looks at digital trust from the point of view of security and examines how valid trust approaches from other domains are now making their way into secure computing. The paper also revisits and analyses the Trusted Platform Module (TPM) along with associated technologies and their relevance in the changing landscape. We especially focus on the domains of cloud computing, mobile computing and cyber-physical systems. In addition, the paper also explores our proposals that are competing with and extending the traditional functionality of TPM specifications.

text: Digital trust - trusted computing and beyond: A position paper Along with the invention of computers and interconnected networks, physical societal notions like security, trust, and privacy entered the digital environment. The concept of digital environments begins with the trust (established in the real world) in the organisation/individual that manages the digital resources. This concept evolved to deal with the rapid growth of the Internet, where it became impractical for entities to have prior offline (real world) trust. The evolution of digital trust took diverse approaches and now trust is defined and understood differently across heterogeneous domains. This paper looks at digital trust from the point of view of security and examines how valid trust approaches from other domains are now making their way into secure computing. The paper also revisits and analyses the Trusted Platform Module (TPM) along with associated technologies and their relevance in the changing landscape. We especially focus on the domains of cloud computing, mobile computing and cyber-physical systems. In addition, the paper also explores our proposals that are competing with and extending the traditional functionality of TPM specifications.

year: 2015-01-15

title: Expression, purification and characterisation of GIGANTEA: A circadian clock-controlled regulator of photoperiodic flowering in plants

authors: Black, Moyra M.

subjects: flowering

abstract: The Arabidopsis thaliana (Arabidopsis) GIGANTEA (GI) gene is a central component of the photoperiodic flowering pathway. While it has been 40 years since the first mutant alleles of GI were described much is still unknown about the molecular mechanism of GI action. To investigate the biochemistry and domain organisation (and ultimately to give a greater understanding of the role of GI in floral induction), it is first necessary to produce significant quantities of purified protein. Soluble affinity-tagged full-length GI was expressed in Escherichia coli (E. coli) and was stabilised by the addition of the detergent n-dodecyl-β-d-maltoside (DDM) to storage and purification buffers. Stabilised GI was purified using a variety of chromatographic methods, and characterised using a selection of biochemical techniques including circular dichroism, and dynamic light scattering. This showed that purified GI contained secondary structure, but was polydisperse in solution. Electron microscopy suggests a possible tetramer arrangement of GI. Limited proteolytic digests and mass spectrometry were used to identify potential GI domains. This led to the identification of a predicted 46 kDa amino-terminal GI domain. GI was also expressed in Sf9 insect cells using the baculovirus expression system. GI produced via this route gave insoluble protein.

text: Expression, purification and characterisation of GIGANTEA: A circadian clock-controlled regulator of photoperiodic flowering in plants The Arabidopsis thaliana (Arabidopsis) GIGANTEA (GI) gene is a central component of the photoperiodic flowering pathway. While it has been 40 years since the first mutant alleles of GI were described much is still unknown about the molecular mechanism of GI action. To investigate the biochemistry and domain organisation (and ultimately to give a greater understanding of the role of GI in floral induction), it is first necessary to produce significant quantities of purified protein. Soluble affinity-tagged full-length GI was expressed in Escherichia coli (E. coli) and was stabilised by the addition of the detergent n-dodecyl-β-d-maltoside (DDM) to storage and purification buffers. Stabilised GI was purified using a variety of chromatographic methods, and characterised using a selection of biochemical techniques including circular dichroism, and dynamic light scattering. This showed that purified GI contained secondary structure, but was polydisperse in solution. Electron microscopy suggests a possible tetramer arrangement of GI. Limited proteolytic digests and mass spectrometry were used to identify potential GI domains. This led to the identification of a predicted 46 kDa amino-terminal GI domain. GI was also expressed in Sf9 insect cells using the baculovirus expression system. GI produced via this route gave insoluble protein.

year: 2010

title: Rethinking English in Māori-medium education

authors: Hill, Richard Kenneth

subjects: Māori-medium

abstract: English language instruction in New Zealand's Māori-medium schools is controversial, with many schools either excluding it from their curriculum or adopting a tokenistic approach. Yet, how Māori-medium educators can best support their students' academic English language growth is still an under-researched and unresolved question. This paper reports on a project involving the English language transition programmes of three Māori-medium primary schools. Interviews and observations of key personnel within these schools revealed the components of their programmes, their perceptions of the need for English transition education and the issues they were facing in implementing English language instruction. The project found a direct relationship between levels of student language attainment and the quantity and quality of exposure to English instruction. Where an English programme occupied a significant place in a school's timetable and was staffed by teachers knowledgeable about the learning needs of bilingual students and how best to attend to these, the result was higher literacy scores and more satisfied students. This paper argues that planning English language outcomes for Māori-medium students is essential if becoming biliterate is an important aim. This planning must also be long-term, across all 13 years of the students' education.

text: Rethinking English in Māori-medium education English language instruction in New Zealand's Māori-medium schools is controversial, with many schools either excluding it from their curriculum or adopting a tokenistic approach. Yet, how Māori-medium educators can best support their students' academic English language growth is still an under-researched and unresolved question. This paper reports on a project involving the English language transition programmes of three Māori-medium primary schools. Interviews and observations of key personnel within these schools revealed the components of their programmes, their perceptions of the need for English transition education and the issues they were facing in implementing English language instruction. The project found a direct relationship between levels of student language attainment and the quantity and quality of exposure to English instruction. Where an English programme occupied a significant place in a school's timetable and was staffed by teachers knowledgeable about the learning needs of bilingual students and how best to attend to these, the result was higher literacy scores and more satisfied students. This paper argues that planning English language outcomes for Māori-medium students is essential if becoming biliterate is an important aim. This planning must also be long-term, across all 13 years of the students' education.

year: 2011