title: Adhesion molecule expression in the bovine mammary gland

authors: Hodgkinson, A.J.

subjects: bovine

abstract: The bovine mammary gland requires lymphocytes for immune protection of the gland from foreign pathogens and, in addition, to transfer immune protection to the neonate via colostrum and milk. The process of homing primed lymphocytes to tissues is mediated by the interaction of cell-adhesion molecules displayed on the surface of lymphocytes and counter receptors displayed on the vascular endothelium. This study was conducted to identify the cell-adhesion molecules involved in homing lymphocytes to the bovine mammary gland at four different physiological stages; pregnant, colostral, lactation and involution. The expression and distribution of adhesion molecules in alveolar tissues and supramammary lymph nodes from the mammary glands of healthy cows was determined in situ by immunohistochemical analysis and compared with bovine Peyer's patch, used as a typical mucosal-associated lymphoid tissue and positive control. The mucosal addressin molecule, MAdCAM-1, was not detected in bovine mammary tissues at any of the four different physiological stages. Absence of MAdCAM-1 expression was verified by quantitative real-time RT-PCR analysis. Transcription levels of MAdCAM-1 mRNA were found to be more then 5 × 103-fold lower in mammary alveolar tissues compared with bovine Peyer's patch tissues. In contrast to MAdCAM-1, phase-dependent protein expression of VCAM-1 was detected in both mammary alveolar tissues and the supramammary lymph nodes, with the highest expression observed in colostral phase cows. The protein expression in mammary alveolar tissues was limited to larger venules, although in colostral phase cows, VCAM-1 was also detected around the alveoli perimeter. In the supramammary lymph node, VCAM-1 protein was observed on both small and large venules. PNAd was detected in supramammary lymph nodes at all physiological stages of the mammary gland; however, it was not found in mammary alveolar tissues. Lymphocytes expressing β7 were not detected in mammary tissues and lymphocytes expressing CD62L were only observed in the supramammary lymph nodes. Overall the data suggest that MAdCAM-1 and VCAM-1 are not involved in homing lymphocytes to the bovine mammary gland; whereas, VCAM-1 and PNAd may have this role in the supramammary lymph node.

text: Adhesion molecule expression in the bovine mammary gland The bovine mammary gland requires lymphocytes for immune protection of the gland from foreign pathogens and, in addition, to transfer immune protection to the neonate via colostrum and milk. The process of homing primed lymphocytes to tissues is mediated by the interaction of cell-adhesion molecules displayed on the surface of lymphocytes and counter receptors displayed on the vascular endothelium. This study was conducted to identify the cell-adhesion molecules involved in homing lymphocytes to the bovine mammary gland at four different physiological stages; pregnant, colostral, lactation and involution. The expression and distribution of adhesion molecules in alveolar tissues and supramammary lymph nodes from the mammary glands of healthy cows was determined in situ by immunohistochemical analysis and compared with bovine Peyer's patch, used as a typical mucosal-associated lymphoid tissue and positive control. The mucosal addressin molecule, MAdCAM-1, was not detected in bovine mammary tissues at any of the four different physiological stages. Absence of MAdCAM-1 expression was verified by quantitative real-time RT-PCR analysis. Transcription levels of MAdCAM-1 mRNA were found to be more then 5 × 103-fold lower in mammary alveolar tissues compared with bovine Peyer's patch tissues. In contrast to MAdCAM-1, phase-dependent protein expression of VCAM-1 was detected in both mammary alveolar tissues and the supramammary lymph nodes, with the highest expression observed in colostral phase cows. The protein expression in mammary alveolar tissues was limited to larger venules, although in colostral phase cows, VCAM-1 was also detected around the alveoli perimeter. In the supramammary lymph node, VCAM-1 protein was observed on both small and large venules. PNAd was detected in supramammary lymph nodes at all physiological stages of the mammary gland; however, it was not found in mammary alveolar tissues. Lymphocytes expressing β7 were not detected in mammary tissues and lymphocytes expressing CD62L were only observed in the supramammary lymph nodes. Overall the data suggest that MAdCAM-1 and VCAM-1 are not involved in homing lymphocytes to the bovine mammary gland; whereas, VCAM-1 and PNAd may have this role in the supramammary lymph node.

year: 2007

title: Intra-industry trade and trade intensities: Evidence from New Zealand

authors: Bano, Sayeeda

subjects: intra-industry international trade

abstract: This study analyses the development of intra-industry and inter industry trade between New Zealand, Australia, and the selected Asia-Pacific nations during the period 1990 to 2000. The study adapts mainly two approaches to examine these developments. First, an historical analysis of New Zealand trading patterns is presented. For this purpose, intra-industry trade development is examined. The Grubel-Lloyd and Aquino indices are used to calculate the intensity of intra- industry trade at the 3-digit SITC levels to determine the relative importance of intra-industry trade as opposed to inter-industry trade. IIT has been estimated across industries and for selected trading partners. A time series approach is used to estimate any trend in the ratio of intra industry trade to total trade in relation to Australia. Secondly, the paper examines the strength of trade relations between New Zealand and the other countries. For this purpose the intensity of trade index has been estimated for bilateral trade flows between these nations. These analyses are examined to consider how trade has changed in this period of trade liberalisation. The results show that intra-industry trade has increased between New Zealand and Australia. The results also suggest that bilateral trade flows between New Zealand, Australia and other countries has become more intense indicating trading relations are strengthening. In some cases bilateral trade flows have decreased. The results also suggest that the removal of trade barriers through bilateral and multilateral negotiations has positive impacts on intra-industry trade and the intensity of trade of these economies.

text: Intra-industry trade and trade intensities: Evidence from New Zealand This study analyses the development of intra-industry and inter industry trade between New Zealand, Australia, and the selected Asia-Pacific nations during the period 1990 to 2000. The study adapts mainly two approaches to examine these developments. First, an historical analysis of New Zealand trading patterns is presented. For this purpose, intra-industry trade development is examined. The Grubel-Lloyd and Aquino indices are used to calculate the intensity of intra- industry trade at the 3-digit SITC levels to determine the relative importance of intra-industry trade as opposed to inter-industry trade. IIT has been estimated across industries and for selected trading partners. A time series approach is used to estimate any trend in the ratio of intra industry trade to total trade in relation to Australia. Secondly, the paper examines the strength of trade relations between New Zealand and the other countries. For this purpose the intensity of trade index has been estimated for bilateral trade flows between these nations. These analyses are examined to consider how trade has changed in this period of trade liberalisation. The results show that intra-industry trade has increased between New Zealand and Australia. The results also suggest that bilateral trade flows between New Zealand, Australia and other countries has become more intense indicating trading relations are strengthening. In some cases bilateral trade flows have decreased. The results also suggest that the removal of trade barriers through bilateral and multilateral negotiations has positive impacts on intra-industry trade and the intensity of trade of these economies.

year: 2002-10

title: Alice Grey Nicholls, daughter of William, and her husband, Charles John Dearle

authors: Hart, Philip

abstract: Alice Grey Nicholls was the ‘half-caste’ daughter of a Pakeha Maori, William Nicholls. She would marry a Pakeha and have several children, losing her only son but bringing up a family of daughters, who all received a Pakeha education. Having a moko emphasized her Maori heritage, and she was on good terms with many Maori.
Charles John Dearle, a Londoner, after some involvement in gold mining spent most of his life working for the government. At the request of Maori landowners, from 1883 until 1895 he allocated goldfields revenue amongst them, a challenging task. He was also involved in land purchases both on behalf of the government and for personal gain. 
They farmed her land at Mangaiti, near Te Aroha, Alice continuing to farm it profitably after his early death, assisted for a time by her daughters. She purchased more land, and to enable her to sell some portions of it she had it declared European land, an illustration of her astuteness in business; her family obtained a good financial position from her farming and land dealings. When she died, aged 81, her Pakeha friends fondly remembered her.

text: Alice Grey Nicholls, daughter of William, and her husband, Charles John Dearle Alice Grey Nicholls was the ‘half-caste’ daughter of a Pakeha Maori, William Nicholls. She would marry a Pakeha and have several children, losing her only son but bringing up a family of daughters, who all received a Pakeha education. Having a moko emphasized her Maori heritage, and she was on good terms with many Maori.
Charles John Dearle, a Londoner, after some involvement in gold mining spent most of his life working for the government. At the request of Maori landowners, from 1883 until 1895 he allocated goldfields revenue amongst them, a challenging task. He was also involved in land purchases both on behalf of the government and for personal gain. 
They farmed her land at Mangaiti, near Te Aroha, Alice continuing to farm it profitably after his early death, assisted for a time by her daughters. She purchased more land, and to enable her to sell some portions of it she had it declared European land, an illustration of her astuteness in business; her family obtained a good financial position from her farming and land dealings. When she died, aged 81, her Pakeha friends fondly remembered her.

year: 2016