title: Application of flow cytometry for examining phytoplankton succession in two eutrophic lakes

authors: Dennis, Marie

subjects: Cyanophyta

abstract: Flow cytometry has potential as a rapid assessment technique to evaluate phytoplankton biomass and species composition. It facilitates for multi-parameter analysis of individual cells on the basis of light scattering effects induced from cellular constituents, as well as auto-fluorescence. Fluorescence emission characteristics may be especially useful in classifying cyanobacteria as they contain phycoerythrin which emits light predominantly in the 550–600 nm waveband, chlorophyll-a (650–700 nm emission) and allophycocyanin (660 nm emission). The objective of our study was to assess the utility of flow cytometry for the rapid identification and sorting of freshwater algae and cyanobacteria species. Using a selection of laboratory-cultured freshwater algae and cyanobacteria species, this study demonstrated unique light scatter and fluorescent characteristics for each species examined, allowing for rapid species identification and sorting of mixed populations of laboratory cultures and samples from two lakes in the Rotorua region (New Zealand). Analysis of lake water samples collected over seven months demonstrated changes in abundance and community composition of phytoplankton in the two lakes and demonstrates that flow cytometry may be a useful technique for examining seasonal changes in phytoplankton composition.

text: Application of flow cytometry for examining phytoplankton succession in two eutrophic lakes Flow cytometry has potential as a rapid assessment technique to evaluate phytoplankton biomass and species composition. It facilitates for multi-parameter analysis of individual cells on the basis of light scattering effects induced from cellular constituents, as well as auto-fluorescence. Fluorescence emission characteristics may be especially useful in classifying cyanobacteria as they contain phycoerythrin which emits light predominantly in the 550–600 nm waveband, chlorophyll-a (650–700 nm emission) and allophycocyanin (660 nm emission). The objective of our study was to assess the utility of flow cytometry for the rapid identification and sorting of freshwater algae and cyanobacteria species. Using a selection of laboratory-cultured freshwater algae and cyanobacteria species, this study demonstrated unique light scatter and fluorescent characteristics for each species examined, allowing for rapid species identification and sorting of mixed populations of laboratory cultures and samples from two lakes in the Rotorua region (New Zealand). Analysis of lake water samples collected over seven months demonstrated changes in abundance and community composition of phytoplankton in the two lakes and demonstrates that flow cytometry may be a useful technique for examining seasonal changes in phytoplankton composition.

year: 2011

title: Guidebook for pre-conference North Island New Zealand 'Volcanoes to Oceans' field tour (26th - 30th July 2010) 19th World Congress of Soil Sciences, Brisbane (31st July - 6 August 2010)

authors: Lowe, David J.

abstract: Field trip sponsored by: Soil and Earth Sciences, INR, Massey University. Department of Earth and Ocean Sciences, University of Waikato. Environment Waikato.

text: Guidebook for pre-conference North Island New Zealand 'Volcanoes to Oceans' field tour (26th - 30th July 2010) 19th World Congress of Soil Sciences, Brisbane (31st July - 6 August 2010) Field trip sponsored by: Soil and Earth Sciences, INR, Massey University. Department of Earth and Ocean Sciences, University of Waikato. Environment Waikato.

year: 2010

title: Kaupapa Māori framework and literature review of key prinicples

authors: Kennedy, Nathan

subjects: New Zealand

abstract: The literature review in this report was the starting point for the development of a Māori research strand within the Planning Under Co-operative Mandates (PUCM) research programme. The original purpose of this report Kaupapa Māori Framework and Literature Review of Key Principles was to establish definitions of environmentally significant concepts of kaupapa and tikanga Māori. In  addition,  the  review  sought  to  identify  and  briefly  describe  significant  variations between  understandings of the key concepts without attempting to reconcile these. As the purpose of the review in 2005 was to inform the development of a kaupapa Māori methodology for the identification and development of Māori environmental outcomes and indicators, we paid particular regard to Māori perceptions of the environment and the relevance of each concept in environmental terms.

text: Kaupapa Māori framework and literature review of key prinicples The literature review in this report was the starting point for the development of a Māori research strand within the Planning Under Co-operative Mandates (PUCM) research programme. The original purpose of this report Kaupapa Māori Framework and Literature Review of Key Principles was to establish definitions of environmentally significant concepts of kaupapa and tikanga Māori. In  addition,  the  review  sought  to  identify  and  briefly  describe  significant  variations between  understandings of the key concepts without attempting to reconcile these. As the purpose of the review in 2005 was to inform the development of a kaupapa Māori methodology for the identification and development of Māori environmental outcomes and indicators, we paid particular regard to Māori perceptions of the environment and the relevance of each concept in environmental terms.

year: 2009-06-30