title: Vascular functioning and development of the kiwifruit berry (Actinidia deliciosa)

authors: Morrison, Daniel

subjects: kiwifruit

abstract: The aim of this study was to understand kiwifruit berry development and the role of cell turgor and the phloem unloading pathway in development. Important aspects of berry development include the size of the fruit and its composition. The fresh weight growth curve of the kiwifruit berry was shown to be double sigmoid in shape. Dry weight accumulated linearly for the initial 139 days after anthesis (DAA). At this time the soluble solids concentration began to increase. Berry firmness was measured using two methods, with the penetrometer and with a new non-destructive method, utilising skinfold callipers. Both methods exhibited similar results, indicating that the skin callipers may be useful in the future for non-destructive berry rheological measurements.

Cell turgor was measured indirectly from measurements of symplasmic and apoplasmic solute potentials, and the matric potential of the berry. Apoplasmic sap, required to measure the apoplasmic solute potential, was extracted using two different methods the pressure chamber and through centrifugation. Measurements of sap osmotic potential suggest that the sap extracted using the centrifuge was contaminated with symplasmic sap, resulting in a negative cell turgor estimate. However, the pressure chamber technique provided apoplasmic sap that produced a more accurate estimate of cell turgor. Direct estimates of cell turgor were only obtained from the midpoint of the growing season because of contamination with symplasmic sap, but the values obtained were comparable to literature values for developing grape and tomato berries.

The phloem unloading pathway in the fruit was investigated using a symplasmic tracer dye, carboxyfluorescein diacetate coupled with 14C labelling and autoradiography. The phloem unloading pathway was symplasmic until 91 DAA when the dye was restricted to the phloem cells only, indicating a change to an apoplasmic pathway. However, due to the lack of functional unloading seen in radiolabelled samples, a change in the phloem unloading pathway could not be confirmed.

text: Vascular functioning and development of the kiwifruit berry (Actinidia deliciosa) The aim of this study was to understand kiwifruit berry development and the role of cell turgor and the phloem unloading pathway in development. Important aspects of berry development include the size of the fruit and its composition. The fresh weight growth curve of the kiwifruit berry was shown to be double sigmoid in shape. Dry weight accumulated linearly for the initial 139 days after anthesis (DAA). At this time the soluble solids concentration began to increase. Berry firmness was measured using two methods, with the penetrometer and with a new non-destructive method, utilising skinfold callipers. Both methods exhibited similar results, indicating that the skin callipers may be useful in the future for non-destructive berry rheological measurements.

Cell turgor was measured indirectly from measurements of symplasmic and apoplasmic solute potentials, and the matric potential of the berry. Apoplasmic sap, required to measure the apoplasmic solute potential, was extracted using two different methods the pressure chamber and through centrifugation. Measurements of sap osmotic potential suggest that the sap extracted using the centrifuge was contaminated with symplasmic sap, resulting in a negative cell turgor estimate. However, the pressure chamber technique provided apoplasmic sap that produced a more accurate estimate of cell turgor. Direct estimates of cell turgor were only obtained from the midpoint of the growing season because of contamination with symplasmic sap, but the values obtained were comparable to literature values for developing grape and tomato berries.

The phloem unloading pathway in the fruit was investigated using a symplasmic tracer dye, carboxyfluorescein diacetate coupled with 14C labelling and autoradiography. The phloem unloading pathway was symplasmic until 91 DAA when the dye was restricted to the phloem cells only, indicating a change to an apoplasmic pathway. However, due to the lack of functional unloading seen in radiolabelled samples, a change in the phloem unloading pathway could not be confirmed.

year: 2012

title: Constructing programs or processes

authors: Reeves, Steve

subjects: cause

abstract: We define interacting sequential programs, motivated originally by constructivist considerations. We use them to investigate notions of implementation and determinism. Process algebras do not define what can be implemented and what cannot. As we demonstrate it is problematic to do so on the set of all processes. Guided by constructivist notions we have constructed interacting sequential programs which we claim can be readily implemented and are a subset of processes.

text: Constructing programs or processes We define interacting sequential programs, motivated originally by constructivist considerations. We use them to investigate notions of implementation and determinism. Process algebras do not define what can be implemented and what cannot. As we demonstrate it is problematic to do so on the set of all processes. Guided by constructivist notions we have constructed interacting sequential programs which we claim can be readily implemented and are a subset of processes.

year: 2005-12-28

title: Te Kāhui a Kiwa—Advancing Indigenous self determination and wellbeing through trade and can the CPTPP help

authors: Mika, Jason

abstract: This report provides an interim account of a conference held in Hamilton on 28-29 June 2023 called Te Kāhui a Kiwa—Advancing Indigenous self-determination and wellbeing through trade and can the CPTPP help. The conference was hosted by the University of Waikato, working collaboratively with Ngā Toki Whakarururanga, with funding from the Ministry of Foreign Affairs and Trade (Manatū Aorere). University kaumātua (elder) Koro Taki Turner gifted the conference its name. Kāhui refers to an assembly of stars or in this case, people, while Kiwa is short for Te Moana nui a Kiwa—the great ocean of Kiwa, the Pacific Ocean.

text: Te Kāhui a Kiwa—Advancing Indigenous self determination and wellbeing through trade and can the CPTPP help This report provides an interim account of a conference held in Hamilton on 28-29 June 2023 called Te Kāhui a Kiwa—Advancing Indigenous self-determination and wellbeing through trade and can the CPTPP help. The conference was hosted by the University of Waikato, working collaboratively with Ngā Toki Whakarururanga, with funding from the Ministry of Foreign Affairs and Trade (Manatū Aorere). University kaumātua (elder) Koro Taki Turner gifted the conference its name. Kāhui refers to an assembly of stars or in this case, people, while Kiwa is short for Te Moana nui a Kiwa—the great ocean of Kiwa, the Pacific Ocean.

year: 2023-07-13