title: Development of models to predict Pinus radiata productivity throughout New Zealand

authors: Watt, Michael S.

subjects: New Zealand

abstract: Development of spatial surfaces describing variation in productivity across broad landscapes at a fine resolution would be of considerable use to forest managers as decision support tools to optimize productivity. In New Zealand, the two most widely used indices to quantify productivity of Pinus radiata D. Don are Site Index and 300 Index. Using an extensive national data set comprising a comprehensive set of national extent maps, multiple regression models and spatial surfaces of these indices for P. radiata were constructed. The final models accounted for 64% and 53%, respectively, of the variance in Site Index and 300 Index. For Site Index, variables included in the final model in order of importance were mean annual air temperature, fractional mean annual available root-zone water storage, mean annual windspeed, length and slope factor, categories describing Land Environments of New Zealand (LENZ), and major soil parent material. The variables included in the final model of 300 Index in order of importance included the degree of ground frost during autumn, fractional mean annual available root-zone water storage, categories describing LENZ, vegetation classification, foliar nitrogen, taxonomic soil order, and major soil parent material. These results highlight the utility of thematic spatial layers as driving variables in the development of productivity models.

text: Development of models to predict Pinus radiata productivity throughout New Zealand Development of spatial surfaces describing variation in productivity across broad landscapes at a fine resolution would be of considerable use to forest managers as decision support tools to optimize productivity. In New Zealand, the two most widely used indices to quantify productivity of Pinus radiata D. Don are Site Index and 300 Index. Using an extensive national data set comprising a comprehensive set of national extent maps, multiple regression models and spatial surfaces of these indices for P. radiata were constructed. The final models accounted for 64% and 53%, respectively, of the variance in Site Index and 300 Index. For Site Index, variables included in the final model in order of importance were mean annual air temperature, fractional mean annual available root-zone water storage, mean annual windspeed, length and slope factor, categories describing Land Environments of New Zealand (LENZ), and major soil parent material. The variables included in the final model of 300 Index in order of importance included the degree of ground frost during autumn, fractional mean annual available root-zone water storage, categories describing LENZ, vegetation classification, foliar nitrogen, taxonomic soil order, and major soil parent material. These results highlight the utility of thematic spatial layers as driving variables in the development of productivity models.

year: 2010

title: Characterization of the potential drug target serine acetyltransferase (CysE) from Acinetobacter baumannii: A Pacific lens on biochemical research

authors: Usu, Jessicca Eva

abstract: Antimicrobial resistance (AMR) presents a critical global health threat, with Acinetobacter baumannii being a key pathogen due to its multidrug resistance. With no new antibiotic classes discovered since the 1980s, novel therapeutic targets are urgently needed. This thesis explores the potential of targeting the cysteine biosynthesis pathway, specifically the enzyme serine acetyltransferase (CysE), as a means to develop innovative antimicrobial strategies. Cysteine plays a pivotal role in bacterial survival and defense, particularly in oxidative stress resistance. Targeting its biosynthesis may impair bacterial virulence and enhance susceptibility to immune responses.

This research focuses on the characterization of CysE from A. baumannii, which uniquely expresses a short and full-length of this enzyme. While we were unable to optimize expression of the short form in this thesis, the long form was successfully expressed, purified, and biochemically and structurally characterized for the first time. Using X-ray crystallography, we present the three-dimensional structure of CysE from A. baumannii to a resolution of 2.14 Å. CysE adopts a homohexameric conformation, forming a dimer of trimers, and features an alpha-helical extension. SAXS analysis validated that the long form exists as a homohexamer in solution. Furthermore, using enzymatic assays we show CysE has serine acetyltransferase activity and is sensitive to feedback inhibition by pathway product L-cysteine, highlighting the regulatory mechanisms of this crucial enzyme. This work improves our understanding of cysteine biosynthesis in A. baumannii and provides a model for structure-based virtual inhibitor screening to identify potential new antimicrobials. 

In addition to its scientific objectives, this research is framed within the Fa'afaletui framework, an Indigenous Samoan methodology that ensures cultural perspectives are integrated into the scientific process. This approach ensures that Pacific values inform the direction of AMR research, fostering solutions that are not only scientifically rigorous but are also culturally relevant and accessible. The research serves to address the health inequities faced by Pacific peoples, particularly in relation to infectious diseases and AMR, and provides a foundation for future antimicrobial drug development efforts.

text: Characterization of the potential drug target serine acetyltransferase (CysE) from Acinetobacter baumannii: A Pacific lens on biochemical research Antimicrobial resistance (AMR) presents a critical global health threat, with Acinetobacter baumannii being a key pathogen due to its multidrug resistance. With no new antibiotic classes discovered since the 1980s, novel therapeutic targets are urgently needed. This thesis explores the potential of targeting the cysteine biosynthesis pathway, specifically the enzyme serine acetyltransferase (CysE), as a means to develop innovative antimicrobial strategies. Cysteine plays a pivotal role in bacterial survival and defense, particularly in oxidative stress resistance. Targeting its biosynthesis may impair bacterial virulence and enhance susceptibility to immune responses.

This research focuses on the characterization of CysE from A. baumannii, which uniquely expresses a short and full-length of this enzyme. While we were unable to optimize expression of the short form in this thesis, the long form was successfully expressed, purified, and biochemically and structurally characterized for the first time. Using X-ray crystallography, we present the three-dimensional structure of CysE from A. baumannii to a resolution of 2.14 Å. CysE adopts a homohexameric conformation, forming a dimer of trimers, and features an alpha-helical extension. SAXS analysis validated that the long form exists as a homohexamer in solution. Furthermore, using enzymatic assays we show CysE has serine acetyltransferase activity and is sensitive to feedback inhibition by pathway product L-cysteine, highlighting the regulatory mechanisms of this crucial enzyme. This work improves our understanding of cysteine biosynthesis in A. baumannii and provides a model for structure-based virtual inhibitor screening to identify potential new antimicrobials. 

In addition to its scientific objectives, this research is framed within the Fa'afaletui framework, an Indigenous Samoan methodology that ensures cultural perspectives are integrated into the scientific process. This approach ensures that Pacific values inform the direction of AMR research, fostering solutions that are not only scientifically rigorous but are also culturally relevant and accessible. The research serves to address the health inequities faced by Pacific peoples, particularly in relation to infectious diseases and AMR, and provides a foundation for future antimicrobial drug development efforts.

year: 2024

title: Māori & Psychology Research Unit Annual Report 2013

authors: Waitoki, Waikaremoana

abstract: Annual report of the Māori and Psychology Research Unit (MPRU) 2013. The unit was established in August of 1997. The unit is designed to provide a catalyst and support network for enhancing research concerning the psychological needs, aspirations, and priorities of Maori people. The MPRU is well situated to draw together skilled and experienced interdisciplinary research groups by networking and establishing working relationships with staff and students within the Faculty of Arts and Social Sciences, the University, and the wider community

text: Māori & Psychology Research Unit Annual Report 2013 Annual report of the Māori and Psychology Research Unit (MPRU) 2013. The unit was established in August of 1997. The unit is designed to provide a catalyst and support network for enhancing research concerning the psychological needs, aspirations, and priorities of Maori people. The MPRU is well situated to draw together skilled and experienced interdisciplinary research groups by networking and establishing working relationships with staff and students within the Faculty of Arts and Social Sciences, the University, and the wider community

year: 2013