title: Molecular Studies of House Mice in Southern New Zealand

authors: McCormick, Helen Margaret

subjects: mus musculus

abstract: The house mouse, Mus Musculus, was first introduced into New Zealand in significant numbers in the mid nineteenth century. Earlier research suggests that multiple introductions of the three subspecies of house mouse M. m. domesticus, M. m. musculus and M. m. castaneus occurred at this time. While M. m. domesticus is widely distributed in New Zealand with M. m. musculus somewhat less so, the only extant evidence found to date for M. m. castaneus is in hybrid mice found principally in the southern half of the South Island. In this study, the hybrid population of mice in the South Island was examined in detail using a variety of molecular techniques. The extent of the hybrid zone was first established using a simple polymerase chain reaction (PCR) technique that enabled rapid identification of mitochondrial genomes as being M. m. domesticus, castaneus or musculus in nature. In the second part of the study, four representative mice from the hybrid zone and three M. m. domesticus from north of this zone were subjected to high density (600K) single nucleotide polymorphism (SNP) analyses in order to pinpoint regions of the genomes that differed in a significant manner between the two groups. In a refinement of these analyses, regions of each genome were identified as being domesticus-, castaneus- or musculus-like, using diagnostic SNP alleles for each sub-species. Some 170 mice, principally collected in the Southern South Island, were screened using the rapid PCR technique. In the coastal regions, all mice further south than 44oS had M. m. castaneus mtDNA whereas those north of 43oS had M. m. domesticus mtDNA. Between the two, there is a ‘contact zone’ in which both subspecies were found, sometimes in the same building. The contact zone extends approximately 50km north to south and some 30km inland. Classical tests with three nuclear DNA markers confirmed earlier work, namely, that the nuclear genomes of all mice appeared to be predominantly domesticus-like. Although there is no obvious geographical, ecological or land-use features that characterise this contact zone it may be relevant that there is the distinct change to a wetter colder climate in the south and inland of the contact zone, especially in winter. The inability to identify obvious defining features is not surprising, given that much research over many years worldwide has failed to yield an explanation for distinct mouse hybrid zones. The high density SNP analyses demonstrated that the nuclear genomes of all seven representative mice were very similar and largely (approximately 95%+) domesticus-like in nature. Despite this similarity, clusters of SNPs did reveal differences throughout the genome, often just extending over a few haplotype blocks and often encompassing much less than a million basepairs (Mb). Some of these were of biological interest, for example clusters of vomeronasal receptor genes and also genes believed to be involved in maternal-fetal conflict, which are known to vary markedly between species. The diagnostic SNP analyses confirmed that no marked differences exist between the genomes of the hybrid and M. m. domesticus mice. As stated above, the genomes were predominantly domesticus-like but small regions (~1Mb) of castaneus-like and musculus-like genome were found scattered throughout all mice but, contrary to initial expectations, there was no preponderance of castaneus-like regions in the hybrid mice. The one exception to the largely identical genomic SNP patterns, were those from the mouse collected in Hamilton. This was quite bizarre, in that its genome contains large (up to 10Mb) musculus-like regions that correspond exactly to the same regions in a common laboratory strain of mouse, C57BL/6J. I conclude that if pure M. m. castaneus mice did originally reach New Zealand, extensive ‘backcrossing’ with M. m. domesticus has virtually eliminated the castaneus genome, with just a few remnants remaining that may or may not confer some selective advantage, but could just as likely represent segregation of recognition factors that give rise to assortative mating. Thus, the most obvious and consistent genetic difference between the mice remains the original observation concerning their mitochondrial genomes and these should be examined in more detail. In future research aimed at identifying potential selective advantages that have allowed the hybrid mice to exclusively populate the southern South Island factors that relate to climate should be considered. Specifically, the fact that, in humans, some mitochondrial haplotypes are believed to confer a selective advantage in colder climates immediately suggests that examining variants of key genes involved in energy metabolism such as NADH-dehydrogenase-subunit-3 ( ND3 ) in the hybrid mice could be a profitable line of future research.

text: Molecular Studies of House Mice in Southern New Zealand The house mouse, Mus Musculus, was first introduced into New Zealand in significant numbers in the mid nineteenth century. Earlier research suggests that multiple introductions of the three subspecies of house mouse M. m. domesticus, M. m. musculus and M. m. castaneus occurred at this time. While M. m. domesticus is widely distributed in New Zealand with M. m. musculus somewhat less so, the only extant evidence found to date for M. m. castaneus is in hybrid mice found principally in the southern half of the South Island. In this study, the hybrid population of mice in the South Island was examined in detail using a variety of molecular techniques. The extent of the hybrid zone was first established using a simple polymerase chain reaction (PCR) technique that enabled rapid identification of mitochondrial genomes as being M. m. domesticus, castaneus or musculus in nature. In the second part of the study, four representative mice from the hybrid zone and three M. m. domesticus from north of this zone were subjected to high density (600K) single nucleotide polymorphism (SNP) analyses in order to pinpoint regions of the genomes that differed in a significant manner between the two groups. In a refinement of these analyses, regions of each genome were identified as being domesticus-, castaneus- or musculus-like, using diagnostic SNP alleles for each sub-species. Some 170 mice, principally collected in the Southern South Island, were screened using the rapid PCR technique. In the coastal regions, all mice further south than 44oS had M. m. castaneus mtDNA whereas those north of 43oS had M. m. domesticus mtDNA. Between the two, there is a ‘contact zone’ in which both subspecies were found, sometimes in the same building. The contact zone extends approximately 50km north to south and some 30km inland. Classical tests with three nuclear DNA markers confirmed earlier work, namely, that the nuclear genomes of all mice appeared to be predominantly domesticus-like. Although there is no obvious geographical, ecological or land-use features that characterise this contact zone it may be relevant that there is the distinct change to a wetter colder climate in the south and inland of the contact zone, especially in winter. The inability to identify obvious defining features is not surprising, given that much research over many years worldwide has failed to yield an explanation for distinct mouse hybrid zones. The high density SNP analyses demonstrated that the nuclear genomes of all seven representative mice were very similar and largely (approximately 95%+) domesticus-like in nature. Despite this similarity, clusters of SNPs did reveal differences throughout the genome, often just extending over a few haplotype blocks and often encompassing much less than a million basepairs (Mb). Some of these were of biological interest, for example clusters of vomeronasal receptor genes and also genes believed to be involved in maternal-fetal conflict, which are known to vary markedly between species. The diagnostic SNP analyses confirmed that no marked differences exist between the genomes of the hybrid and M. m. domesticus mice. As stated above, the genomes were predominantly domesticus-like but small regions (~1Mb) of castaneus-like and musculus-like genome were found scattered throughout all mice but, contrary to initial expectations, there was no preponderance of castaneus-like regions in the hybrid mice. The one exception to the largely identical genomic SNP patterns, were those from the mouse collected in Hamilton. This was quite bizarre, in that its genome contains large (up to 10Mb) musculus-like regions that correspond exactly to the same regions in a common laboratory strain of mouse, C57BL/6J. I conclude that if pure M. m. castaneus mice did originally reach New Zealand, extensive ‘backcrossing’ with M. m. domesticus has virtually eliminated the castaneus genome, with just a few remnants remaining that may or may not confer some selective advantage, but could just as likely represent segregation of recognition factors that give rise to assortative mating. Thus, the most obvious and consistent genetic difference between the mice remains the original observation concerning their mitochondrial genomes and these should be examined in more detail. In future research aimed at identifying potential selective advantages that have allowed the hybrid mice to exclusively populate the southern South Island factors that relate to climate should be considered. Specifically, the fact that, in humans, some mitochondrial haplotypes are believed to confer a selective advantage in colder climates immediately suggests that examining variants of key genes involved in energy metabolism such as NADH-dehydrogenase-subunit-3 ( ND3 ) in the hybrid mice could be a profitable line of future research.

year: 2011

title: The 'glow up' imperative: The fitness lifestyles of young women in Aotearoa New Zealand

authors: Kurghan, Francesca

subjects: body image

abstract: For many young women, their relationships with fitness, health and the body are shaped by unrealistic beauty ideals. Body image scholars have long focused on the impacts of the media and more recently, social media, on the way young women experience body satisfaction. This thesis investigates the lived experiences of young women in Aotearoa New Zealand, in relation to body image and fitness culture. Underpinned by feminist post-structuralist theory, this research project aims to amplify the voices and experiences of young women (18-25 years) to gain nuanced insights into how they interpret, internalise and negotiate the complexities of the fitness lifestyles and everyday body image. This study draws upon an anonymous survey (203 participants), as well as three focus groups and three individual interviews with 15 young women, with all participants considering themselves to be actively pursuing fitness lifestyles.

Conducting a thematic analysis, results showed that many young women are actively pursuing a range of fitness practices for diverse reasons and motives. However, many report feeling the pressure not only to adhere to thin ideals, but also to pursue bodies that are strong and toned. For many of the young women, however, the fitness lifestyles were also connected to broader social trends of ‘fitspo’ (fitness inspiration) and the ‘glow up’ (body and lifestyle transformation). Engaging with feminist literature on the pressures on young women to aspire and achieve ‘successful’ femininities in the context of neoliberalism, this research develops the concept of the ‘glow up’ in relation to their highly consumptive fitness lifestyles. In so doing, this thesis provides rich, nuanced and original insights into the pressures on everyday young women to pursue bodily and lifestyle transformation, and the effects on those unable to maintain their commitment to the ‘glow up’ imperative. Importantly, not all young women passively accept such limiting versions of the ‘body project’, with some actively acknowledging the harm that can be caused by the ‘glow up’ imperative. The thesis concludes with some researcher reflections on the contributions of this study and future directions to help young women redefine what it means to participate in meaningful physical activity.

text: The 'glow up' imperative: The fitness lifestyles of young women in Aotearoa New Zealand For many young women, their relationships with fitness, health and the body are shaped by unrealistic beauty ideals. Body image scholars have long focused on the impacts of the media and more recently, social media, on the way young women experience body satisfaction. This thesis investigates the lived experiences of young women in Aotearoa New Zealand, in relation to body image and fitness culture. Underpinned by feminist post-structuralist theory, this research project aims to amplify the voices and experiences of young women (18-25 years) to gain nuanced insights into how they interpret, internalise and negotiate the complexities of the fitness lifestyles and everyday body image. This study draws upon an anonymous survey (203 participants), as well as three focus groups and three individual interviews with 15 young women, with all participants considering themselves to be actively pursuing fitness lifestyles.

Conducting a thematic analysis, results showed that many young women are actively pursuing a range of fitness practices for diverse reasons and motives. However, many report feeling the pressure not only to adhere to thin ideals, but also to pursue bodies that are strong and toned. For many of the young women, however, the fitness lifestyles were also connected to broader social trends of ‘fitspo’ (fitness inspiration) and the ‘glow up’ (body and lifestyle transformation). Engaging with feminist literature on the pressures on young women to aspire and achieve ‘successful’ femininities in the context of neoliberalism, this research develops the concept of the ‘glow up’ in relation to their highly consumptive fitness lifestyles. In so doing, this thesis provides rich, nuanced and original insights into the pressures on everyday young women to pursue bodily and lifestyle transformation, and the effects on those unable to maintain their commitment to the ‘glow up’ imperative. Importantly, not all young women passively accept such limiting versions of the ‘body project’, with some actively acknowledging the harm that can be caused by the ‘glow up’ imperative. The thesis concludes with some researcher reflections on the contributions of this study and future directions to help young women redefine what it means to participate in meaningful physical activity.

year: 2024

title: Ko Te Kawa Tūpanapana i ngā Hau Tūpua a Tāwhiri-mātea : The validation, revitalisation and enhancement of Māori environment knowledge of weather and climate

authors: Skipper, Apanui Sonny

subjects: Maori Environmental knowledge

abstract: KO TE KAWA TŪPANAPANA I NGĀ HAU TŪPUA A TĀWHIRI-MĀTEA – THE RITUALISTIC FORMS GOVERNING THE SUPERNATURAL WINDS OF TĀWHIRI-MĀTEA
Mankind has always gazed skyward observing the im immense power generated by forked lightning when it strikes the land. The resounding thunder booming across the landscape never failed to capture the awe and imagination of a people. Māori were no different. The legacy of their Polynesian ancestors, who populated most of the Islands of Te Moana-nui-a-Kiwa, the largest ocean on the planet, based on belief, courage, and navigational expertise, honed over many millenia, reached Aotearoa approximately in the 12 century. These highly skilled ancestors from the tropics applied a formalised process of settling a new land. It also required their own weatherlore to be recalibrated to the local conditions, in order to survive in a much harsher, colder climate.

Over the centuries the descendants of these early Polynesian settlers, developed an extensive localised knowledge of weather, climate, and seasonal indicators to accurately predict daily weather, monthly or annual climate conditions. The ongoing lessons learned especially after experiencing a fatality were incorporated into cultural practices of important everyday activities such as ahuwhenua, te mahi hī ika, kohi rongoā, whakaako tamariki, gathering pounamu, whakatū rāhui, and kaitiakitanga, in order to minimise further loss of life.

Formal recognition of these contributions was made at the inaugural Māori Climate Forum in Wellington in 2003, where a number of Māori elders highlighted the importance of giving a greater account of Māori knowledge of environmental change. Among the many priorities identified for research at this forum, an opportunity based on a challenge from a Māori elder led to the development of a pilot project to explore traditional Māori understandings of weather–climate variability and change. 

One of the key outcomes from this work was the identification of Māori environmental indicators to anticipate local weather and climate conditions and thereby manage the risk associated with weather-climate extremes. However, in spite of the work achieved, there remain unanswered questions about the use and ongoing efficacy of environmental indicators to forecast and monitor weather and climate risks, including how new opportunities might be created to promote learning about subtle signals in nature that can reveal much about changes in weather and climate conditions. 

Due to the fact that many of the elders that provided the basis of this thesis have passed away, a great sense of responsibility has remained with me to complete this research. To identify the gaps in the previous research, to ground-truth it, to consolidate and carefully document the level of localised environmental knowledge of weather and climate specifically with iwi from Tainui-Hauraki, Te Whānau-a-Apanui, and Ngāi Tahu. A total of 56 interviews and 17 group wānanga were conducted during 2009-2016. Examples of Māori Environmental Knowledge were also sourced from numerous personal conversations with knowledge holders from 1989-2003, adding to the body of knowledge that was analysed to provide further context. A conceptual framework coined `Ngā Whenu Tapu e Waru’ was created from identifying the key themes of the interviews to inform how Māori Environmental Knowledge was described in the three regions. This study has yielded understandings of wind, rain, thunder, lightning, and cloud classifications; weather and climate indicators; spiritual signs of misfortune and death; memory techniques; longitudinal weather predictions, and lunar calendars in each region.

This thesis is underpinned by a Kaupapa Māori theory and research methodology that links to a body of transformative research and literature conducted by Māori scholars based on the knowledge and experience of tūpuna  Māori. 

Heoti anō rā kei ngā kākahi whakairoiro, kei ngā matataiko, nau piki mai, nau kake mai ki tēnei wānanga nui whakaharahara.

text: Ko Te Kawa Tūpanapana i ngā Hau Tūpua a Tāwhiri-mātea : The validation, revitalisation and enhancement of Māori environment knowledge of weather and climate KO TE KAWA TŪPANAPANA I NGĀ HAU TŪPUA A TĀWHIRI-MĀTEA – THE RITUALISTIC FORMS GOVERNING THE SUPERNATURAL WINDS OF TĀWHIRI-MĀTEA
Mankind has always gazed skyward observing the im immense power generated by forked lightning when it strikes the land. The resounding thunder booming across the landscape never failed to capture the awe and imagination of a people. Māori were no different. The legacy of their Polynesian ancestors, who populated most of the Islands of Te Moana-nui-a-Kiwa, the largest ocean on the planet, based on belief, courage, and navigational expertise, honed over many millenia, reached Aotearoa approximately in the 12 century. These highly skilled ancestors from the tropics applied a formalised process of settling a new land. It also required their own weatherlore to be recalibrated to the local conditions, in order to survive in a much harsher, colder climate.

Over the centuries the descendants of these early Polynesian settlers, developed an extensive localised knowledge of weather, climate, and seasonal indicators to accurately predict daily weather, monthly or annual climate conditions. The ongoing lessons learned especially after experiencing a fatality were incorporated into cultural practices of important everyday activities such as ahuwhenua, te mahi hī ika, kohi rongoā, whakaako tamariki, gathering pounamu, whakatū rāhui, and kaitiakitanga, in order to minimise further loss of life.

Formal recognition of these contributions was made at the inaugural Māori Climate Forum in Wellington in 2003, where a number of Māori elders highlighted the importance of giving a greater account of Māori knowledge of environmental change. Among the many priorities identified for research at this forum, an opportunity based on a challenge from a Māori elder led to the development of a pilot project to explore traditional Māori understandings of weather–climate variability and change. 

One of the key outcomes from this work was the identification of Māori environmental indicators to anticipate local weather and climate conditions and thereby manage the risk associated with weather-climate extremes. However, in spite of the work achieved, there remain unanswered questions about the use and ongoing efficacy of environmental indicators to forecast and monitor weather and climate risks, including how new opportunities might be created to promote learning about subtle signals in nature that can reveal much about changes in weather and climate conditions. 

Due to the fact that many of the elders that provided the basis of this thesis have passed away, a great sense of responsibility has remained with me to complete this research. To identify the gaps in the previous research, to ground-truth it, to consolidate and carefully document the level of localised environmental knowledge of weather and climate specifically with iwi from Tainui-Hauraki, Te Whānau-a-Apanui, and Ngāi Tahu. A total of 56 interviews and 17 group wānanga were conducted during 2009-2016. Examples of Māori Environmental Knowledge were also sourced from numerous personal conversations with knowledge holders from 1989-2003, adding to the body of knowledge that was analysed to provide further context. A conceptual framework coined `Ngā Whenu Tapu e Waru’ was created from identifying the key themes of the interviews to inform how Māori Environmental Knowledge was described in the three regions. This study has yielded understandings of wind, rain, thunder, lightning, and cloud classifications; weather and climate indicators; spiritual signs of misfortune and death; memory techniques; longitudinal weather predictions, and lunar calendars in each region.

This thesis is underpinned by a Kaupapa Māori theory and research methodology that links to a body of transformative research and literature conducted by Māori scholars based on the knowledge and experience of tūpuna  Māori. 

Heoti anō rā kei ngā kākahi whakairoiro, kei ngā matataiko, nau piki mai, nau kake mai ki tēnei wānanga nui whakaharahara.

year: 2020