title: Development of a molecular tool to identify brown bullhead catfish (Ameiurus nebulosus) from environmental DNA in water

authors: Hicks, Brendan J.

abstract: The aim of this work was to develop a molecular tool to positively identify brown bullhead catfish using environmental DNA (eDNA) that is suspended within the water column. The project had five main objectives: 1) to develop a protocol for the collection of eDNA, 2) to select and test primer sets that are specific to brown bullhead catfish, 3) to optimise DNA extraction and amplification, 4) to validate the methodology through sequencing the amplified DNA, and 5) to evaluate its sensitivity by sampling at various sites that had a range of catfish abundances.

Water was sampled from Lake Rotoiti from a boat with a 1 litre labelled plastic container attached to a telescopic pole, retrieving water samples about 30 cm above the lake bottom in about 2 m of water depth and from deeper water. Each container was deployed container-opening facing down, and on reaching the desired collection depth, the opening was rotated uppermost to allow the entry of water. The full container was then pulled back to the surface, a plastic screw cap firmly screwed onto the container, and then subsequently placed in a chilly bin on a 20-cm layer of crushed ice. The collection was recorded on a GPS instrument.

text: Development of a molecular tool to identify brown bullhead catfish (Ameiurus nebulosus) from environmental DNA in water The aim of this work was to develop a molecular tool to positively identify brown bullhead catfish using environmental DNA (eDNA) that is suspended within the water column. The project had five main objectives: 1) to develop a protocol for the collection of eDNA, 2) to select and test primer sets that are specific to brown bullhead catfish, 3) to optimise DNA extraction and amplification, 4) to validate the methodology through sequencing the amplified DNA, and 5) to evaluate its sensitivity by sampling at various sites that had a range of catfish abundances.

Water was sampled from Lake Rotoiti from a boat with a 1 litre labelled plastic container attached to a telescopic pole, retrieving water samples about 30 cm above the lake bottom in about 2 m of water depth and from deeper water. Each container was deployed container-opening facing down, and on reaching the desired collection depth, the opening was rotated uppermost to allow the entry of water. The full container was then pulled back to the surface, a plastic screw cap firmly screwed onto the container, and then subsequently placed in a chilly bin on a 20-cm layer of crushed ice. The collection was recorded on a GPS instrument.

year: 2020-08-21

title: Functional ecology of an Antarctic Dry Valley

authors: Chan, Yuki

abstract: The McMurdo Dry Valleys are the largest ice-free region in Antarctica and are critically at risk from climate change. The terrestrial landscape is dominated by oligotrophic mineral soils and extensive exposed rocky surfaces where biota are largely restricted to microbial communities, although their ability to perform the majority of geobiological processes has remained largely uncharacterized. Here, we identified functional traits that drive microbial survival and community assembly, using a metagenomic approach with GeoChip-based functional gene arrays to establish metabolic capabilities in communities inhabiting soil and rock surface niches in McKelvey Valley. Major pathways in primary metabolism were identified, indicating significant plasticity in autotrophic, heterotrophic, and diazotrophic strategies supporting microbial communities. This represents a major advance beyond biodiversity surveys in that we have now identified how putative functional ecology drives microbial community assembly. Significant differences were apparent between open soil, hypolithic, chasmoendolithic, and cryptoendolithic communities. A suite of previously unappreciated Antarctic microbial stress response pathways, thermal, osmotic, and nutrient limitation responses were identified and related to environmental stressors, offering tangible clues to the mechanisms behind the enduring success of microorganisms in this seemingly inhospitable terrain. Rocky substrates exposed to larger fluctuations in environmental stress supported greater functional diversity in stress-response pathways than soils. Soils comprised a unique reservoir of genes involved in transformation of organic hydrocarbons and lignin-like degradative pathways. This has major implications for the evolutionary origin of the organisms, turnover of recalcitrant substrates in Antarctic soils, and predicting future responses to anthropogenic pollution.

text: Functional ecology of an Antarctic Dry Valley The McMurdo Dry Valleys are the largest ice-free region in Antarctica and are critically at risk from climate change. The terrestrial landscape is dominated by oligotrophic mineral soils and extensive exposed rocky surfaces where biota are largely restricted to microbial communities, although their ability to perform the majority of geobiological processes has remained largely uncharacterized. Here, we identified functional traits that drive microbial survival and community assembly, using a metagenomic approach with GeoChip-based functional gene arrays to establish metabolic capabilities in communities inhabiting soil and rock surface niches in McKelvey Valley. Major pathways in primary metabolism were identified, indicating significant plasticity in autotrophic, heterotrophic, and diazotrophic strategies supporting microbial communities. This represents a major advance beyond biodiversity surveys in that we have now identified how putative functional ecology drives microbial community assembly. Significant differences were apparent between open soil, hypolithic, chasmoendolithic, and cryptoendolithic communities. A suite of previously unappreciated Antarctic microbial stress response pathways, thermal, osmotic, and nutrient limitation responses were identified and related to environmental stressors, offering tangible clues to the mechanisms behind the enduring success of microorganisms in this seemingly inhospitable terrain. Rocky substrates exposed to larger fluctuations in environmental stress supported greater functional diversity in stress-response pathways than soils. Soils comprised a unique reservoir of genes involved in transformation of organic hydrocarbons and lignin-like degradative pathways. This has major implications for the evolutionary origin of the organisms, turnover of recalcitrant substrates in Antarctic soils, and predicting future responses to anthropogenic pollution.

year: 2013

title: E Toru Ngā Reo: A Case Study of a Spanish Language Programme in a Kura Kaupapa Māori

authors: Flavell, William

subjects: Kaupapa Maori Research

abstract: Learning Languages is a new area in the 2007 New Zealand Curriculum.  However there have not been many studies in this subject.  Kura Kaupapa Māori, established in 1985 in New Zealand with over 6,000 students currently attending, have English language programmes (Hill, 2010), however few, if any, studies of the teaching of other languages have been conducted.  The purpose of this study was to describe and evaluate an additional international language programme at a Kura Kaupapa Māori.

This qualitative case study describes the views of the Principal, five teachers, five students and three parents of a Kura Kaupapa Māori about an additional Spanish language programme. The primary means of gathering data was through individual semi-structured interviews.  Interviews were used to collect data in the areas of identifying the attitudes of the participants towards learning an additional international language, the perceived benefits, if any, of learning an additional international language and if there is a relationship between the learning of an  additional international language, and Kaupapa Māori.  The narrative data was transcribed, coded and categorised into four themes related to the research questions.  

 The success of this Spanish language programme can be understood in terms of several factors such as whānau support, programme leadership, quality teachers, international excursions and positive attitudes to foreign language learning.  

These findings will contribute to literature concerning additional language learning programmes, specifically those based in indigenous language immersion settings and they will also provide useful information and ideas which other Kura Kaupapa Māori can take advantage of when implementing additional international language learning programmes.

text: E Toru Ngā Reo: A Case Study of a Spanish Language Programme in a Kura Kaupapa Māori Learning Languages is a new area in the 2007 New Zealand Curriculum.  However there have not been many studies in this subject.  Kura Kaupapa Māori, established in 1985 in New Zealand with over 6,000 students currently attending, have English language programmes (Hill, 2010), however few, if any, studies of the teaching of other languages have been conducted.  The purpose of this study was to describe and evaluate an additional international language programme at a Kura Kaupapa Māori.

This qualitative case study describes the views of the Principal, five teachers, five students and three parents of a Kura Kaupapa Māori about an additional Spanish language programme. The primary means of gathering data was through individual semi-structured interviews.  Interviews were used to collect data in the areas of identifying the attitudes of the participants towards learning an additional international language, the perceived benefits, if any, of learning an additional international language and if there is a relationship between the learning of an  additional international language, and Kaupapa Māori.  The narrative data was transcribed, coded and categorised into four themes related to the research questions.  

 The success of this Spanish language programme can be understood in terms of several factors such as whānau support, programme leadership, quality teachers, international excursions and positive attitudes to foreign language learning.  

These findings will contribute to literature concerning additional language learning programmes, specifically those based in indigenous language immersion settings and they will also provide useful information and ideas which other Kura Kaupapa Māori can take advantage of when implementing additional international language learning programmes.

year: 2012