title: Thermostable cellobiohydrolase from the thermophilic eubacterium Thermotoga sp. strain FjSS3-B.1. Purification and properties.

authors: Ruttersmith, L.D.

subjects: biology

abstract: Exo-1,4-beta-cellobiohydrolase (EC 3.2.1.91) was isolated from the culture supernatant of Thermotoga sp. strain FjSS3-B.1, an extremely thermophilic eubacterium that grows optimally at 80 degrees C. The enzyme was purified to homogeneity as determined by SDS/PAGE and has an Mr of 36,000. The enzyme is the most thermostable cellulase reported to date, with a half-life at 108 degrees C of 70 min in buffer. In a 40 min assay, maximal activity was recorded at 105 degrees C. Cellobiohydrolase from strain FjSS3-B.1 is active against amorphous cellulose and CM-cellulose but only effects limited hydrolysis of filter paper or Sigmacell 20. The only product identified by h.p.l.c. is the disaccharide cellobiose. The enzyme has a pH optimum around neutral and is stabilized by the presence of 0.8 M-NaCl.

text: Thermostable cellobiohydrolase from the thermophilic eubacterium Thermotoga sp. strain FjSS3-B.1. Purification and properties. Exo-1,4-beta-cellobiohydrolase (EC 3.2.1.91) was isolated from the culture supernatant of Thermotoga sp. strain FjSS3-B.1, an extremely thermophilic eubacterium that grows optimally at 80 degrees C. The enzyme was purified to homogeneity as determined by SDS/PAGE and has an Mr of 36,000. The enzyme is the most thermostable cellulase reported to date, with a half-life at 108 degrees C of 70 min in buffer. In a 40 min assay, maximal activity was recorded at 105 degrees C. Cellobiohydrolase from strain FjSS3-B.1 is active against amorphous cellulose and CM-cellulose but only effects limited hydrolysis of filter paper or Sigmacell 20. The only product identified by h.p.l.c. is the disaccharide cellobiose. The enzyme has a pH optimum around neutral and is stabilized by the presence of 0.8 M-NaCl.

year: 1991

title: Book review: Native Shakespeares: Indigenous Appropriations on a Global Stage. The Shakespearean International Yearbook, vol.9: Specialsection, South African Shakespeare in the Twentieth Century. Chinese Shakespeares: Two Centuries of Cultural Exchange.

authors: Houlahan, Mark

subjects: book review

abstract: This article reviews three books: “Native Shakespeares: Indigenous Appropriations on a Global Stage” edited by Craig Dionne and Parmita Kapadia, “The Shakespearean International Yearbook, vol. 9: Special section, South African Shakespeare in the Twentieth Century” Special guest ed., Laurence Wright; gen. eds., Graham Bradshaw and Tom Bishop, and “Chinese Shakespeares: Two Centuries of Cultural Exchange.” by Alexander C. Y. Huang.

text: Book review: Native Shakespeares: Indigenous Appropriations on a Global Stage. The Shakespearean International Yearbook, vol.9: Specialsection, South African Shakespeare in the Twentieth Century. Chinese Shakespeares: Two Centuries of Cultural Exchange. This article reviews three books: “Native Shakespeares: Indigenous Appropriations on a Global Stage” edited by Craig Dionne and Parmita Kapadia, “The Shakespearean International Yearbook, vol. 9: Special section, South African Shakespeare in the Twentieth Century” Special guest ed., Laurence Wright; gen. eds., Graham Bradshaw and Tom Bishop, and “Chinese Shakespeares: Two Centuries of Cultural Exchange.” by Alexander C. Y. Huang.

year: 2011-05

title: The TOHU framework: Sensing and interpreting tohu to heal from trauma

authors: McLachlan, A

subjects: Environment

abstract: This hypotheses article presents understandings and practices of tohu (signs) in the personal, eco-environmental, and spiritual wellbeing of Māori. Tohu can be observed in the natural, social, physical, or spiritual environment, and within a Māori worldview provide important ways of understanding and responding to phenomena. Wānanga (shared dialogue and debate) were held with seven Māori clinical psychologists from He Paiaka Tōtara (Māori Psychologist Network) and two mātauranga Māori specialists to explore their experiences, knowledge, and perspectives about tohu, with their insights collected through online information sharing. Our wānanga goals were to inform the creation of a therapeutic framework to address patu ngākau, psychological and spiritual trauma experienced by Māori. The results identified that tohu are located within the person and their environment, and the importance of exploring how tohu are interpreted. We propose a TOHU acronym as a framework for understanding and addressing the multifaceted impacts of patu ngākau.

text: The TOHU framework: Sensing and interpreting tohu to heal from trauma This hypotheses article presents understandings and practices of tohu (signs) in the personal, eco-environmental, and spiritual wellbeing of Māori. Tohu can be observed in the natural, social, physical, or spiritual environment, and within a Māori worldview provide important ways of understanding and responding to phenomena. Wānanga (shared dialogue and debate) were held with seven Māori clinical psychologists from He Paiaka Tōtara (Māori Psychologist Network) and two mātauranga Māori specialists to explore their experiences, knowledge, and perspectives about tohu, with their insights collected through online information sharing. Our wānanga goals were to inform the creation of a therapeutic framework to address patu ngākau, psychological and spiritual trauma experienced by Māori. The results identified that tohu are located within the person and their environment, and the importance of exploring how tohu are interpreted. We propose a TOHU acronym as a framework for understanding and addressing the multifaceted impacts of patu ngākau.

year: 2024