title: Genome sequence of temperate bacteriophage Psymv2 from Antarctic dry valley soil isolate Psychrobacter sp. MV2

authors: Meiring, Tracy L.

subjects: Psychrobacter

abstract: A temperate phage, Psymv2, was isolated from an Antarctic soil bacterium, Psychrobacter sp. MV2. The morphology of Psymv2 was typical of the Siphoviridae, with an isometric head and non-contractile tail. The Psymv2 genome was found to be 35,725 bp in length, had a G + C content of 44.5 %, with 49 protein-coding genes and one tRNA gene predicted. Integration of Psymv2 occurred at an ssrA gene, with the last 27 bases of this gene directly repeated at the prophage ends. The genome was organised in a modular fashion: integration, regulation, packaging, head assembly, tail assembly, host specificity and lysis. While the genome sequence had little similarity on a nucleotide level to previously reported phage sequences, the genome architecture resembled that of Siphoviridae of low G + C Gram-positive bacteria. The closest relatives to Psymv2 were uncharacterized putative prophages within the P. arcticus 273-4 and Acinetobacter baumannii 6013113 genomes. Global alignment of the Psymv2 genome and these prophages revealed significant conservation of the structural modules despite the large spatial divergence of their hosts. A number of unique ORFs were identified in the Psymv2 genome that may contribute to phage and lysogen fitness.

text: Genome sequence of temperate bacteriophage Psymv2 from Antarctic dry valley soil isolate Psychrobacter sp. MV2 A temperate phage, Psymv2, was isolated from an Antarctic soil bacterium, Psychrobacter sp. MV2. The morphology of Psymv2 was typical of the Siphoviridae, with an isometric head and non-contractile tail. The Psymv2 genome was found to be 35,725 bp in length, had a G + C content of 44.5 %, with 49 protein-coding genes and one tRNA gene predicted. Integration of Psymv2 occurred at an ssrA gene, with the last 27 bases of this gene directly repeated at the prophage ends. The genome was organised in a modular fashion: integration, regulation, packaging, head assembly, tail assembly, host specificity and lysis. While the genome sequence had little similarity on a nucleotide level to previously reported phage sequences, the genome architecture resembled that of Siphoviridae of low G + C Gram-positive bacteria. The closest relatives to Psymv2 were uncharacterized putative prophages within the P. arcticus 273-4 and Acinetobacter baumannii 6013113 genomes. Global alignment of the Psymv2 genome and these prophages revealed significant conservation of the structural modules despite the large spatial divergence of their hosts. A number of unique ORFs were identified in the Psymv2 genome that may contribute to phage and lysogen fitness.

year: 2012

title: Towards the digital music library: tune retrieval from acoustic input

authors: McNab, Rodger J.

subjects: computer science

abstract: Music is traditionally retrieved by title, composer or subject classification. It is possible, with current technology, to retrieve music from a database on the basis of a few notes sung or hummed into a microphone. This paper describes the implementation of such a system, and discusses several issues pertaining to music retrieval. We first describe an interface that transcribes acoustic input into standard music notation. We then analyze string matching requirements from ranked retrieval of music and present the results of an experiment which tests how accurately people sing well known melodies. The performance of several string matching criteria are analyzed using two folk song databases. Finally, we describe a prototype system which has been developed for retrieval of tunes from acoustic input.

text: Towards the digital music library: tune retrieval from acoustic input Music is traditionally retrieved by title, composer or subject classification. It is possible, with current technology, to retrieve music from a database on the basis of a few notes sung or hummed into a microphone. This paper describes the implementation of such a system, and discusses several issues pertaining to music retrieval. We first describe an interface that transcribes acoustic input into standard music notation. We then analyze string matching requirements from ranked retrieval of music and present the results of an experiment which tests how accurately people sing well known melodies. The performance of several string matching criteria are analyzed using two folk song databases. Finally, we describe a prototype system which has been developed for retrieval of tunes from acoustic input.

year: 1995-10

title: The art of dialogue with indigenous communities in the new biotechnology world

authors: Hudson, Maui

subjects: biotechnology

abstract: Te Hau Mihi Ata is a research project that aims to negotiate spaces for and develop processes of dialogue that allow for a deeper level of interaction between mtauranga Māori (Māori indigenous knowledge) and science. Over a two-year period a series of facilitated exchanges or wnanga were held focusing on areas of new technology (assisted reproductive technologies, life technologies, and future food technologies) that involved Māori scientists and people with expertise in mtauranga Māori. These dialogue events were designed to explore, through the challenge of considering new biotechnologies, the similarities and differences that emerge from approaching these issues from different knowledge paradigms. This paper will outline the key project findings from this series of exchanges including the barriers and facilitators to the dialogue process and knowledge exchange as well as discuss how participants created connections between scientific discourse and indigenous knowledge frameworks.

text: The art of dialogue with indigenous communities in the new biotechnology world Te Hau Mihi Ata is a research project that aims to negotiate spaces for and develop processes of dialogue that allow for a deeper level of interaction between mtauranga Māori (Māori indigenous knowledge) and science. Over a two-year period a series of facilitated exchanges or wnanga were held focusing on areas of new technology (assisted reproductive technologies, life technologies, and future food technologies) that involved Māori scientists and people with expertise in mtauranga Māori. These dialogue events were designed to explore, through the challenge of considering new biotechnologies, the similarities and differences that emerge from approaching these issues from different knowledge paradigms. This paper will outline the key project findings from this series of exchanges including the barriers and facilitators to the dialogue process and knowledge exchange as well as discuss how participants created connections between scientific discourse and indigenous knowledge frameworks.

year: 2012