title: The thermal behaviour of enzyme activity: implications for biotechnology

authors: Eisenthal, Robert

subjects: enzyme

abstract: The way that enzymes respond to temperature is fundamental to many areas of biotechnology. This has long been explained in terms of enzyme stability and catalytic activation energy, but recent observations of enzyme behaviour suggest that this picture is incomplete. We have developed and experimentally validated a new model to describe the effect of temperature on enzymes; this model incorporates additional fundamental parameters that enable a complete description of the effects of temperature on enzyme activity. In this article, we consider the biotechnological implications of this model in the areas of enzyme engineering, enzyme reactor operation and the selection and/or screening of useful enzymes from the environment.

text: The thermal behaviour of enzyme activity: implications for biotechnology The way that enzymes respond to temperature is fundamental to many areas of biotechnology. This has long been explained in terms of enzyme stability and catalytic activation energy, but recent observations of enzyme behaviour suggest that this picture is incomplete. We have developed and experimentally validated a new model to describe the effect of temperature on enzymes; this model incorporates additional fundamental parameters that enable a complete description of the effects of temperature on enzyme activity. In this article, we consider the biotechnological implications of this model in the areas of enzyme engineering, enzyme reactor operation and the selection and/or screening of useful enzymes from the environment.

year: 2006

title: Oceanographic and morphodynamic monitoring of a multi-purpose reef at Mount Maunganui, New Zealand

authors: Weppe, Simon Bastien

subjects: multi-purpose reef

abstract: The innovative concept of an offshore submerged multi-purpose reef combining a
coastal protection function with recreational benefits including improved surfing,
diving, or fishing conditions provides an appealing solution for the management
of beaches. However, since the technology is relatively recent, there is still a lack
of empirical knowledge about its performance in the field. The present research
monitored the effect of the prototype  research  reef constructed at Mount
Maunganui in New Zealand on the local beach morphodynamics and
oceanographic conditions, being primarily concerned with implications on the
coastal protection function of the technology.
The beach morphodynamic response to the reef was investigated from a set of
already available high resolution bathymetric surveys imaging the foreshore and
surfzone morphology prior to, and throughout reef construction, and a new postconstruction
survey collected as a part of this research. The reef implementation
was found to disturb the pre-existing beach state functioning including the
onshore/offshore migration of the underlying long shore bar, rather than cause a
persistent salient response. A possible additional beach width of ~ 20 m,
extending ~ 150 m alongshore was identified in the lee of the reef from shoreline
analysis, but the pattern was transient throughout the monitoring period since it
was superimposed on comparatively large pre-existing fluctuations. In addition,
the reef structure provided a control point on the offshore morphology able to trap
sediment updrift and erode sediment downdrift.
Both field measurements and numerical modelling of waves and currents were
used to monitor the effect of the reef on the oceanographic conditions. Wave
propagation over the reef without breaking (H less than 0.5 m) resulted in transmitted
heights larger than incident by up to a factor of 2. When the reef triggered
breaking, transmitted heights were reduced by up to ~40 %. A concurrent process
was the shift of the wave energy spectra towards higher frequencies landward of
the reef that resulted in reduced transmitted wave periods. Wave modelling
showed that the wave shadowing was associated with significant wave rotation
around the reef that induced two zones of divergent wave angles near the
shoreline in the lee of the reef. The hydrodynamic response to the wave energy
dissipation was the development of stronger onshore directed currents landward of
the reef. Hydrodynamic modelling indicated that the reef-induced flow forced a
cellular circulation in the lee side under shore normal waves, and an onshore
deviation of the ambient (unidirectional) long-shore currents under oblique waves.
The research provided a rare empirical test to the theoretical design concepts, and
potential supplements or refinements. To obtain the required beach protection, the
reef impact on the beach morphodynamic coupling including wave
transformation, nearshore hydrodynamics, and small/large scale beach state
response needs to be carefully assessed.

text: Oceanographic and morphodynamic monitoring of a multi-purpose reef at Mount Maunganui, New Zealand The innovative concept of an offshore submerged multi-purpose reef combining a
coastal protection function with recreational benefits including improved surfing,
diving, or fishing conditions provides an appealing solution for the management
of beaches. However, since the technology is relatively recent, there is still a lack
of empirical knowledge about its performance in the field. The present research
monitored the effect of the prototype  research  reef constructed at Mount
Maunganui in New Zealand on the local beach morphodynamics and
oceanographic conditions, being primarily concerned with implications on the
coastal protection function of the technology.
The beach morphodynamic response to the reef was investigated from a set of
already available high resolution bathymetric surveys imaging the foreshore and
surfzone morphology prior to, and throughout reef construction, and a new postconstruction
survey collected as a part of this research. The reef implementation
was found to disturb the pre-existing beach state functioning including the
onshore/offshore migration of the underlying long shore bar, rather than cause a
persistent salient response. A possible additional beach width of ~ 20 m,
extending ~ 150 m alongshore was identified in the lee of the reef from shoreline
analysis, but the pattern was transient throughout the monitoring period since it
was superimposed on comparatively large pre-existing fluctuations. In addition,
the reef structure provided a control point on the offshore morphology able to trap
sediment updrift and erode sediment downdrift.
Both field measurements and numerical modelling of waves and currents were
used to monitor the effect of the reef on the oceanographic conditions. Wave
propagation over the reef without breaking (H less than 0.5 m) resulted in transmitted
heights larger than incident by up to a factor of 2. When the reef triggered
breaking, transmitted heights were reduced by up to ~40 %. A concurrent process
was the shift of the wave energy spectra towards higher frequencies landward of
the reef that resulted in reduced transmitted wave periods. Wave modelling
showed that the wave shadowing was associated with significant wave rotation
around the reef that induced two zones of divergent wave angles near the
shoreline in the lee of the reef. The hydrodynamic response to the wave energy
dissipation was the development of stronger onshore directed currents landward of
the reef. Hydrodynamic modelling indicated that the reef-induced flow forced a
cellular circulation in the lee side under shore normal waves, and an onshore
deviation of the ambient (unidirectional) long-shore currents under oblique waves.
The research provided a rare empirical test to the theoretical design concepts, and
potential supplements or refinements. To obtain the required beach protection, the
reef impact on the beach morphodynamic coupling including wave
transformation, nearshore hydrodynamics, and small/large scale beach state
response needs to be carefully assessed.

year: 2010

title: "Ko au te whenua, ko te whenua ko au:" A Māori housing development process

authors: Fletcher, Marcus Anzac Kewene

subjects: Māori

abstract: Tangata whenua (People of the land/Indigenous people of Aotearoa) are being recognised as emerging property and land developers in Aotearoa New Zealand (hereafter, Aotearoa). Literature and practice demonstrates how Māori are developing housing in different ways than traditional developers, supporting the wellbeing of whānau (families), hapū (sub-tribes) and iwi (tribes), while contributing to addressing the national housing crisis. The distinctiveness of these approaches are important to understand if we want to deepen our knowledge of urban development and the modelling of housing markets in Aotearoa. This thesis aims to reveal, codify and examine Māori decision-making processes relating to how and why we develop housing in Aotearoa and the values and principles that inform these approaches. Utilising Kaupapa Māori Theory and taking an insider/whānaunga (relatives) approach to the research, four whakawhiti kōrero sessions with Waikato whānau and kaimahi (workers) were undertaken to better understand how and why Māori develop housing and the processes and rationalities at play. The thesis has three contributions for academia, environmental planning and the housing sector in Aotearoa: insights into the values and rationalities that influence Māori urban development; the introduction of a Māori housing development framework; and insights into how the framework may be utilised to contribute towards more accurate and sophisticated agent-based modelling (ABM) or urban growth modelling.  

The findings and discussion identified six prominent values that Māori utilise to develop housing: kaitiakitanga, manaakitanga, orangatanga, whānaungatanga, ūkaipōtanga and rangatiratanga. Being rooted in Māori knowledge and epistemology essentially demands differing rationalities for when Māori undertake housing development, which are placed within an overarching framework. The framework represents a new tool to disrupt the current understanding of ‘developers’ as a single entity with a defined pathway that influences all development behaviour. Moreover, it demonstrates how whānau, hapū and iwi draw on the established learning and guidance of our tūpuna (ancestors) and the strides taken to reclaim tino rangatiratanga (self-determination). The thesis argues that academics, environmental practitioners and the housing sector should re-examine their understanding of developers in Aotearoa to become more pluralistic, which may present new challenges and opportunities for ABM and urban growth modelling. This framework argues some aspects may have potential to be incorporated into these technical analyses, while other aspects should be considered in the context of Māori and Indigenous Data Sovereignty to protect the contributions and mana of the participants within this thesis, or resist the ability to be captured altogether.

text: "Ko au te whenua, ko te whenua ko au:" A Māori housing development process Tangata whenua (People of the land/Indigenous people of Aotearoa) are being recognised as emerging property and land developers in Aotearoa New Zealand (hereafter, Aotearoa). Literature and practice demonstrates how Māori are developing housing in different ways than traditional developers, supporting the wellbeing of whānau (families), hapū (sub-tribes) and iwi (tribes), while contributing to addressing the national housing crisis. The distinctiveness of these approaches are important to understand if we want to deepen our knowledge of urban development and the modelling of housing markets in Aotearoa. This thesis aims to reveal, codify and examine Māori decision-making processes relating to how and why we develop housing in Aotearoa and the values and principles that inform these approaches. Utilising Kaupapa Māori Theory and taking an insider/whānaunga (relatives) approach to the research, four whakawhiti kōrero sessions with Waikato whānau and kaimahi (workers) were undertaken to better understand how and why Māori develop housing and the processes and rationalities at play. The thesis has three contributions for academia, environmental planning and the housing sector in Aotearoa: insights into the values and rationalities that influence Māori urban development; the introduction of a Māori housing development framework; and insights into how the framework may be utilised to contribute towards more accurate and sophisticated agent-based modelling (ABM) or urban growth modelling.  

The findings and discussion identified six prominent values that Māori utilise to develop housing: kaitiakitanga, manaakitanga, orangatanga, whānaungatanga, ūkaipōtanga and rangatiratanga. Being rooted in Māori knowledge and epistemology essentially demands differing rationalities for when Māori undertake housing development, which are placed within an overarching framework. The framework represents a new tool to disrupt the current understanding of ‘developers’ as a single entity with a defined pathway that influences all development behaviour. Moreover, it demonstrates how whānau, hapū and iwi draw on the established learning and guidance of our tūpuna (ancestors) and the strides taken to reclaim tino rangatiratanga (self-determination). The thesis argues that academics, environmental practitioners and the housing sector should re-examine their understanding of developers in Aotearoa to become more pluralistic, which may present new challenges and opportunities for ABM and urban growth modelling. This framework argues some aspects may have potential to be incorporated into these technical analyses, while other aspects should be considered in the context of Māori and Indigenous Data Sovereignty to protect the contributions and mana of the participants within this thesis, or resist the ability to be captured altogether.

year: 2025