title: Digital trust - trusted computing and beyond: A position paper

authors: Akram, Raja Naeem

subjects: Cloud computing

abstract: Along with the invention of computers and interconnected networks, physical societal notions like security, trust, and privacy entered the digital environment. The concept of digital environments begins with the trust (established in the real world) in the organisation/individual that manages the digital resources. This concept evolved to deal with the rapid growth of the Internet, where it became impractical for entities to have prior offline (real world) trust. The evolution of digital trust took diverse approaches and now trust is defined and understood differently across heterogeneous domains. This paper looks at digital trust from the point of view of security and examines how valid trust approaches from other domains are now making their way into secure computing. The paper also revisits and analyses the Trusted Platform Module (TPM) along with associated technologies and their relevance in the changing landscape. We especially focus on the domains of cloud computing, mobile computing and cyber-physical systems. In addition, the paper also explores our proposals that are competing with and extending the traditional functionality of TPM specifications.

text: Digital trust - trusted computing and beyond: A position paper Along with the invention of computers and interconnected networks, physical societal notions like security, trust, and privacy entered the digital environment. The concept of digital environments begins with the trust (established in the real world) in the organisation/individual that manages the digital resources. This concept evolved to deal with the rapid growth of the Internet, where it became impractical for entities to have prior offline (real world) trust. The evolution of digital trust took diverse approaches and now trust is defined and understood differently across heterogeneous domains. This paper looks at digital trust from the point of view of security and examines how valid trust approaches from other domains are now making their way into secure computing. The paper also revisits and analyses the Trusted Platform Module (TPM) along with associated technologies and their relevance in the changing landscape. We especially focus on the domains of cloud computing, mobile computing and cyber-physical systems. In addition, the paper also explores our proposals that are competing with and extending the traditional functionality of TPM specifications.

year: 2015-01-15

title: Community resilience: A multidisciplinary exploration for inclusive strategies and scalable solutions

authors: Boston, Megan

abstract: This paper evaluates literature across multiple disciplines and stakeholder types to identify commonalities and contradictions in definitions for community resilience. It aims to support cross-disciplinary discourse to build an interdisciplinary understanding of community resilience. This work identifies the differences between mono-, multi-, inter-, and cross-disciplinary approaches to inform community resilience strategies in academic and practice-based contexts. Four themes for community resilience were identified through a review of cross-disciplinary literature. These include (1) diverse yet convergent definitions of community resilience and the evolution from equilibrium to adaptation to transformation; (2) equitable and inclusive strategies for the development of community resilience initiatives; (3) when and at what scale strategies should be implemented; and (4) community resilience as a process or an outcome. This work is valuable to those seeking to familiarise themselves with the concept of community resilience, including educators who deliver courses on community resilience and policy-makers. It is novel in that it presents an interdisciplinary framework for navigating the community resilience discourse beyond individual professional boundaries.

text: Community resilience: A multidisciplinary exploration for inclusive strategies and scalable solutions This paper evaluates literature across multiple disciplines and stakeholder types to identify commonalities and contradictions in definitions for community resilience. It aims to support cross-disciplinary discourse to build an interdisciplinary understanding of community resilience. This work identifies the differences between mono-, multi-, inter-, and cross-disciplinary approaches to inform community resilience strategies in academic and practice-based contexts. Four themes for community resilience were identified through a review of cross-disciplinary literature. These include (1) diverse yet convergent definitions of community resilience and the evolution from equilibrium to adaptation to transformation; (2) equitable and inclusive strategies for the development of community resilience initiatives; (3) when and at what scale strategies should be implemented; and (4) community resilience as a process or an outcome. This work is valuable to those seeking to familiarise themselves with the concept of community resilience, including educators who deliver courses on community resilience and policy-makers. It is novel in that it presents an interdisciplinary framework for navigating the community resilience discourse beyond individual professional boundaries.

year: 2024-03

title: In vitro cell culture to study microglial inflammation

authors: Newland, Julia

subjects: CNs

abstract: Inflammation of the central nervous system (CNS) characterised by release of cytokines such as tumour necrosis factor-alpha (TNF-a) and interleukin-1 (IL-1) is an event that is often detrimental to neuronal cells and is thought to contribute to the neuronal loss observed during neurodegeneration.
One objective of this study was to investigate the potential anti-inflammatory properties of kawakawa leaf extracts using in vitro model cell systems. Kawakawa leaf extracts anti-inflammatory properties have anecdotally been documented in traditional Maori medicinal practices (Rangoa). However, before this aspect could be carried out, experiments were undertaken to investigate the effects the kawakawa leaf extracts had on the growth rates of two mammalian cell lines (HeLa and THP-1 cells).
Interestingly, the growth assays showed that the extracts to have both a stimulatory and inhibitory effect on cell growth. Toxicity of cells can be categorised as either necrotic or apoptotic cell death. In this study, HeLa cells and THP-1 cells were incubated under standard growth conditions with various concentrations (10 to 100ug/ml) of Kawakawa leave extracts prepared at room temperature or 60OC.
Growth data as seen in figures 2 and 3 typically have a long lag phase before they start to reach exponential growth between days 4 and 5. The extract having the largest stimulatory effect on HeLa cells as shown by figure 6 was 25 ug/ml at 60OC and the largest inhibitory effect seen on HeLa cells was seen at 50 ug/ml RT shown by figure 6. The extract which has the largest stimulatory effect on THP-1 cells is 25 ug/ml 60OC as shown by figure 11 whereas the extract producing the largest inhibitory effect is 25 ug/ml RT.

Apoptotic and necrotic cell death mechanisms were examined by using in vitro biochemistry techniques. Assessments were largely made based on biochemical changes of HeLa cells and THP-1 cells post treatment of Kawakawa leave extracts.
Measurement of lactate dehydrogenase (LDH assays) in the growth media of cells that were shown to be growth inhibited during exposure to Kawakawa extracts indicated that the growth inhibition resulting from exposure to the extracts was not due to necrotic cell death processes. (ie that cells maintained their membrane integrity) When compared to the control, a lower amount of cytosolic LDH was seen in THP-1 cell lines. However, HeLa cell lines produced a high level of cytosolic LDH which may be due to mycoplasma contamination. On average, Kawakawa concentrations at 12.5ug/ml and 25ug/ml yielded less lysed cells percentage across both cell lines.
The demonstrated cell growth inhibition was unlikely to be due to necrosis and because of this it was decided to see if cell death was caused by mitochondrial processes that would further support apoptotic cell death. The biochemical assay MTT indirectly measures mitochondrial dehydrogenase activity in the presence of kawakawa leave extracts.
The MTT data illustrated mitochondrial impairment where HeLa cells exposed to Kawakawa 60OC concentrations 6.25 ug/ml and 12.5 ug/ml had the lowest average mitochondrial activity. THP-1 cells exposed to Kawakawa 60OC concentrations 12.5 ug/ml and 50 ug/ml had the lowest average mitochondrial activity.
The main results of this study include, the treatment of HeLa and THP-1 cells with kawakawa extracts demonstrated that cytotoxicity was not due to necrosis. The most interesting finding of this study is when kawakawa extracts inhibit cell growth there is no increase in LDH. This is suggestive that the cellular membrane remains intact when exposed to the extract.

text: In vitro cell culture to study microglial inflammation Inflammation of the central nervous system (CNS) characterised by release of cytokines such as tumour necrosis factor-alpha (TNF-a) and interleukin-1 (IL-1) is an event that is often detrimental to neuronal cells and is thought to contribute to the neuronal loss observed during neurodegeneration.
One objective of this study was to investigate the potential anti-inflammatory properties of kawakawa leaf extracts using in vitro model cell systems. Kawakawa leaf extracts anti-inflammatory properties have anecdotally been documented in traditional Maori medicinal practices (Rangoa). However, before this aspect could be carried out, experiments were undertaken to investigate the effects the kawakawa leaf extracts had on the growth rates of two mammalian cell lines (HeLa and THP-1 cells).
Interestingly, the growth assays showed that the extracts to have both a stimulatory and inhibitory effect on cell growth. Toxicity of cells can be categorised as either necrotic or apoptotic cell death. In this study, HeLa cells and THP-1 cells were incubated under standard growth conditions with various concentrations (10 to 100ug/ml) of Kawakawa leave extracts prepared at room temperature or 60OC.
Growth data as seen in figures 2 and 3 typically have a long lag phase before they start to reach exponential growth between days 4 and 5. The extract having the largest stimulatory effect on HeLa cells as shown by figure 6 was 25 ug/ml at 60OC and the largest inhibitory effect seen on HeLa cells was seen at 50 ug/ml RT shown by figure 6. The extract which has the largest stimulatory effect on THP-1 cells is 25 ug/ml 60OC as shown by figure 11 whereas the extract producing the largest inhibitory effect is 25 ug/ml RT.

Apoptotic and necrotic cell death mechanisms were examined by using in vitro biochemistry techniques. Assessments were largely made based on biochemical changes of HeLa cells and THP-1 cells post treatment of Kawakawa leave extracts.
Measurement of lactate dehydrogenase (LDH assays) in the growth media of cells that were shown to be growth inhibited during exposure to Kawakawa extracts indicated that the growth inhibition resulting from exposure to the extracts was not due to necrotic cell death processes. (ie that cells maintained their membrane integrity) When compared to the control, a lower amount of cytosolic LDH was seen in THP-1 cell lines. However, HeLa cell lines produced a high level of cytosolic LDH which may be due to mycoplasma contamination. On average, Kawakawa concentrations at 12.5ug/ml and 25ug/ml yielded less lysed cells percentage across both cell lines.
The demonstrated cell growth inhibition was unlikely to be due to necrosis and because of this it was decided to see if cell death was caused by mitochondrial processes that would further support apoptotic cell death. The biochemical assay MTT indirectly measures mitochondrial dehydrogenase activity in the presence of kawakawa leave extracts.
The MTT data illustrated mitochondrial impairment where HeLa cells exposed to Kawakawa 60OC concentrations 6.25 ug/ml and 12.5 ug/ml had the lowest average mitochondrial activity. THP-1 cells exposed to Kawakawa 60OC concentrations 12.5 ug/ml and 50 ug/ml had the lowest average mitochondrial activity.
The main results of this study include, the treatment of HeLa and THP-1 cells with kawakawa extracts demonstrated that cytotoxicity was not due to necrosis. The most interesting finding of this study is when kawakawa extracts inhibit cell growth there is no increase in LDH. This is suggestive that the cellular membrane remains intact when exposed to the extract.

year: 2016