title: Blu-ray cytometer

authors: Albugami, Naser

abstract: The flow cytometers are one of the most powerful devices used to measure the properties of individual particles passing through a sensing region. In this thesis, a low cost cytometer using a Blu-ray pickup is analysed and further developed. This includes the characterisation of the existing system and its performance as well as the evaluation of count rate for different flow rates and focuses. Based on that, the best parameters for measurement conditions have been determined. The system was set up for forward scatter as well as for fluorescence, both were to be measured at the same time and it was found that both could be recorded and counted. In addition, it was found that fluorescing particles can give a fluorescent count as well as scatter count and it was noticed that different sizes of non-fluorescent particles can produce scatter count. The count rate for a mixture of fluorescent and non-fluorescent particles was measured. This required the installation of two different amplifiers (for fluorescent and non-fluorescent particles) and building a new LabView program containing two channels.

In a later part of the thesis, the system was improved to progress towards a setup of avalanche photodiode as detector for forward scattering as well as fluorescence. This gives a very good counting accuracy of 90% at speed of 10μl/s. However, this counting accuracy drops to 80% at speed of 1μl/s when mixing fluorescent and non-fluorescent particles. The counting accuracy is the ratio (in percent) of the measured count to the actual one. As a new detection approach, a beam stop was installed in front of the photo detector so scattered laser light from particles was detected instead of a reduction of laser intensity. This part of the project proves Mie theory which states that the the forward scattered light is in the forward direction. To double check this result, a beam pass was installed in front of the photo detector and the calculated percentage of counting accuracy was very close to the one without a beam stop. It was also checked that the system was capable of detection fluorescent particle after the installation of a high bass filter which passes fluorescent wavelength signals (green) and blocks the blue ray signals. Finally, it was found that this system is able to detect small size particles, such as 1μm particles and to distinguish between 1μm and 10μm particles.

text: Blu-ray cytometer The flow cytometers are one of the most powerful devices used to measure the properties of individual particles passing through a sensing region. In this thesis, a low cost cytometer using a Blu-ray pickup is analysed and further developed. This includes the characterisation of the existing system and its performance as well as the evaluation of count rate for different flow rates and focuses. Based on that, the best parameters for measurement conditions have been determined. The system was set up for forward scatter as well as for fluorescence, both were to be measured at the same time and it was found that both could be recorded and counted. In addition, it was found that fluorescing particles can give a fluorescent count as well as scatter count and it was noticed that different sizes of non-fluorescent particles can produce scatter count. The count rate for a mixture of fluorescent and non-fluorescent particles was measured. This required the installation of two different amplifiers (for fluorescent and non-fluorescent particles) and building a new LabView program containing two channels.

In a later part of the thesis, the system was improved to progress towards a setup of avalanche photodiode as detector for forward scattering as well as fluorescence. This gives a very good counting accuracy of 90% at speed of 10μl/s. However, this counting accuracy drops to 80% at speed of 1μl/s when mixing fluorescent and non-fluorescent particles. The counting accuracy is the ratio (in percent) of the measured count to the actual one. As a new detection approach, a beam stop was installed in front of the photo detector so scattered laser light from particles was detected instead of a reduction of laser intensity. This part of the project proves Mie theory which states that the the forward scattered light is in the forward direction. To double check this result, a beam pass was installed in front of the photo detector and the calculated percentage of counting accuracy was very close to the one without a beam stop. It was also checked that the system was capable of detection fluorescent particle after the installation of a high bass filter which passes fluorescent wavelength signals (green) and blocks the blue ray signals. Finally, it was found that this system is able to detect small size particles, such as 1μm particles and to distinguish between 1μm and 10μm particles.

year: 2012

title: That's 'é' not 'þ' '?' or '☐': a user-driven context-aware approach to erroneous metadata in digital libraries

authors: Bainbridge, David

subjects: computer science

abstract: In this paper we present a novel system for user-driven integration of name variants when interacting with web-based information systems. The growth and diversity of online information means that many users experience disambiguation and collocation errors in their information searching. We approach these issues via a client-side JavaScript browser extension that can reorganise web content and also integrate remote data sources. The system is illustrated through three worked examples using existing digital libraries.

text: That's 'é' not 'þ' '?' or '☐': a user-driven context-aware approach to erroneous metadata in digital libraries In this paper we present a novel system for user-driven integration of name variants when interacting with web-based information systems. The growth and diversity of online information means that many users experience disambiguation and collocation errors in their information searching. We approach these issues via a client-side JavaScript browser extension that can reorganise web content and also integrate remote data sources. The system is illustrated through three worked examples using existing digital libraries.

year: 2011

title: The embodied and lived experiences of welfare and care for highly impaired, high performance para-sport athletes in Aotearoa New Zealand

authors: Lowry, Amanda

abstract: Aligning with calls in critical disability studies to bring the body and impairment into disability sport, this thesis examines the welfare and care experiences of highly impaired athletes in high-performance sport. While a focus on athlete welfare has gained some international prominence, and a duty of care is increasingly invoked as an organisational and coaching responsibility, these developments remain largely disconnected from disability.  
The research is centred around two main objectives. First, to amplify the lived and embodied experiences of highly impaired, high performance disabled athletes as they prepare for, train, and compete in sport, and those who support them. Second, to understand how sporting institutions and regulations (within the broader context of government funding and national health care provision) influence welfare and care practices for high performance disabled athletes. 
Crip methodologies and Indigenous Māori storytelling methodologies such as pūrākau centres lived experience as a site of resistance and knowledge production. Using a reflexive, qualitative approach, including semi-structured interviews, autoethnographic vignettes, and visual ethnography, I expose how impairment effects are managed in ableist sporting environments that valorise normative athlete ideals. I interviewed 11 high-performance paraathletes, seven organisational representatives and two support workers. 
The research highlights the material, emotional, and temporal labour of care in highperformance sport. Findings are organised around three core themes: the embodied labour of care, impairment effects and interdependence; the tension between crip time, care time, and performance time; the intersecting structural, institutional, and ableist barriers that shape and constrain highly impaired athletes’ participation in disability sport.  
The first theme shows how high-performance sport privileges autonomous, efficient, and normative bodies, while marginalising those whose messy, gritty embodied impairment effects demand more time, support, and interdependence. The findings bring to the fore biosocial, psycho-emotional costs of navigating care and its impact on athlete welfare. The second theme uncovers how the rhythms of highly impaired bodies disrupt the linear, clockbound time of high-performance sport, revealing the incompatibility between care time and high performance cultures. It highlights the vital yet invisible labour of support workers, ii unacknowledged in contemporary sporting discourse. The third theme addresses the barriers that highly impaired athletes face when navigating ableist institutional and organisational structures. It exposes the complexity of the ableist disability sporting landscape and the gap between duty of care rhetoric and practice. 
Ultimately, this thesis calls for a radical reimagining of disability sport, one that centres interdependence, affirms bodily difference, and addresses the structural failures that marginalise highly impaired athletes.

text: The embodied and lived experiences of welfare and care for highly impaired, high performance para-sport athletes in Aotearoa New Zealand Aligning with calls in critical disability studies to bring the body and impairment into disability sport, this thesis examines the welfare and care experiences of highly impaired athletes in high-performance sport. While a focus on athlete welfare has gained some international prominence, and a duty of care is increasingly invoked as an organisational and coaching responsibility, these developments remain largely disconnected from disability.  
The research is centred around two main objectives. First, to amplify the lived and embodied experiences of highly impaired, high performance disabled athletes as they prepare for, train, and compete in sport, and those who support them. Second, to understand how sporting institutions and regulations (within the broader context of government funding and national health care provision) influence welfare and care practices for high performance disabled athletes. 
Crip methodologies and Indigenous Māori storytelling methodologies such as pūrākau centres lived experience as a site of resistance and knowledge production. Using a reflexive, qualitative approach, including semi-structured interviews, autoethnographic vignettes, and visual ethnography, I expose how impairment effects are managed in ableist sporting environments that valorise normative athlete ideals. I interviewed 11 high-performance paraathletes, seven organisational representatives and two support workers. 
The research highlights the material, emotional, and temporal labour of care in highperformance sport. Findings are organised around three core themes: the embodied labour of care, impairment effects and interdependence; the tension between crip time, care time, and performance time; the intersecting structural, institutional, and ableist barriers that shape and constrain highly impaired athletes’ participation in disability sport.  
The first theme shows how high-performance sport privileges autonomous, efficient, and normative bodies, while marginalising those whose messy, gritty embodied impairment effects demand more time, support, and interdependence. The findings bring to the fore biosocial, psycho-emotional costs of navigating care and its impact on athlete welfare. The second theme uncovers how the rhythms of highly impaired bodies disrupt the linear, clockbound time of high-performance sport, revealing the incompatibility between care time and high performance cultures. It highlights the vital yet invisible labour of support workers, ii unacknowledged in contemporary sporting discourse. The third theme addresses the barriers that highly impaired athletes face when navigating ableist institutional and organisational structures. It exposes the complexity of the ableist disability sporting landscape and the gap between duty of care rhetoric and practice. 
Ultimately, this thesis calls for a radical reimagining of disability sport, one that centres interdependence, affirms bodily difference, and addresses the structural failures that marginalise highly impaired athletes.

year: 2025-10