title: Identifying Host Species of Dactylanthus taylorii using DNA Barcoding

authors: Parker, Cassarndra Marie

subjects: Dactylanthus

abstract: The population of the endangered endemic holoparasitic plant, Dactylanthus taylorii Hook F. (Balanophoraceae), in Pureora Forest Park was selected as the trial population for developing a method to accurately identify host species using DNA barcoding methods. The marker used was psbA-trnH, and ten samples were able to be successfully identified using the protocol trialed. Of these samples, nine were identified as Pseudopanax arboreus, with one result of Podocarpus totara. The marker used (psbA-trnH) provided adequate levels of variation to discriminate between the host species sequenced in the pilot study. Further work will focus on refining the protocol and trialing a second region for greater resolution of the Pseudopanax species cluster. The method used is relatively simple to employ, and resulted in no mortality of host or tuber. The ability to accurately identify hosts paves the way for the creation of an accurate host list which reflects the true host range and preferences of Dactylanthus taylorii. Globally, few host lists are accurate, and this poses significant challenges for managers. Future work will expand upon this study, collecting host root samples from populations across New Zealand to determine host preferences.

text: Identifying Host Species of Dactylanthus taylorii using DNA Barcoding The population of the endangered endemic holoparasitic plant, Dactylanthus taylorii Hook F. (Balanophoraceae), in Pureora Forest Park was selected as the trial population for developing a method to accurately identify host species using DNA barcoding methods. The marker used was psbA-trnH, and ten samples were able to be successfully identified using the protocol trialed. Of these samples, nine were identified as Pseudopanax arboreus, with one result of Podocarpus totara. The marker used (psbA-trnH) provided adequate levels of variation to discriminate between the host species sequenced in the pilot study. Further work will focus on refining the protocol and trialing a second region for greater resolution of the Pseudopanax species cluster. The method used is relatively simple to employ, and resulted in no mortality of host or tuber. The ability to accurately identify hosts paves the way for the creation of an accurate host list which reflects the true host range and preferences of Dactylanthus taylorii. Globally, few host lists are accurate, and this poses significant challenges for managers. Future work will expand upon this study, collecting host root samples from populations across New Zealand to determine host preferences.

year: 2015

title: Identification and structure elucidation of epoxyjanthitrems from Lolium perenne infected with the endophytic fungus Epichloë festucae var. lolii and determination of the tremorgenic and anti-insect activity of epoxyjanthitrem I.

authors: Finch, Sarah C.

subjects: AR37

abstract: Epoxyjanthitrems I-IV (1-4) and epoxyjanthitriol (5) were isolated from seed of perennial ryegrass (Lolium perenne) infected with the endophytic fungus Epichloë festucae var. lolii. Although structures for epoxyjanthitrems I-IV have previously been proposed in the literature, this is the first report of a full structural elucidation yielding NMR (Nuclear magnetic resonance) assignments for all five epoxyjanthitrem compounds, and additionally, it is the first isolation of epoxyjanthitriol (5). Epoxyjanthitrem I induced tremors in mice and gave a dose dependent reduction in weight gain and feeding for porina (Wiseana cervinata), a common pasture pest in New Zealand. These data suggest that epoxyjanthitrems are involved in the observed effects of the AR37 endophyte on livestock and insect pests.

text: Identification and structure elucidation of epoxyjanthitrems from Lolium perenne infected with the endophytic fungus Epichloë festucae var. lolii and determination of the tremorgenic and anti-insect activity of epoxyjanthitrem I. Epoxyjanthitrems I-IV (1-4) and epoxyjanthitriol (5) were isolated from seed of perennial ryegrass (Lolium perenne) infected with the endophytic fungus Epichloë festucae var. lolii. Although structures for epoxyjanthitrems I-IV have previously been proposed in the literature, this is the first report of a full structural elucidation yielding NMR (Nuclear magnetic resonance) assignments for all five epoxyjanthitrem compounds, and additionally, it is the first isolation of epoxyjanthitriol (5). Epoxyjanthitrem I induced tremors in mice and gave a dose dependent reduction in weight gain and feeding for porina (Wiseana cervinata), a common pasture pest in New Zealand. These data suggest that epoxyjanthitrems are involved in the observed effects of the AR37 endophyte on livestock and insect pests.

year: 2020-08-17

title: Māori & Psychology Research Unit Annual Report 2012

authors: Waitoki, Waikaremoana

abstract: Annual report of the Māori and Psychology Research Unit (MPRU) 2012. The unit was established in August of 1997. The unit is designed to provide a catalyst and support network for enhancing research concerning the psychological needs, aspirations, and priorities of Maori people. The MPRU is well situated to draw together skilled and experienced interdisciplinary research groups by networking and establishing working relationships with staff and students within the Faculty of Arts and Social Sciences, the University, and the wider community.

text: Māori & Psychology Research Unit Annual Report 2012 Annual report of the Māori and Psychology Research Unit (MPRU) 2012. The unit was established in August of 1997. The unit is designed to provide a catalyst and support network for enhancing research concerning the psychological needs, aspirations, and priorities of Maori people. The MPRU is well situated to draw together skilled and experienced interdisciplinary research groups by networking and establishing working relationships with staff and students within the Faculty of Arts and Social Sciences, the University, and the wider community.

year: 2012