title: The use of gas–phase substrates to study enzyme catalysis at low hydration

authors: Dunn, Rachel V.

subjects: enzyme

abstract: Although there are varying estimates as to the degree of enzyme hydration required for activity, a threshold value of ca. 0.2 g of water per gram of protein has been widely accepted. The evidence upon which this is based is reviewed here. In particular, results from the use of gas–phase substrates are discussed. Results using solid–phase enzyme–substrate mixtures are not altogether in accord with those obtained using gas–phase substrates. The use of gaseous substrates and products provides an experimental system in which the hydration of the enzyme can be easily controlled, but which is not limited by diffusion. 
All the results show that increasing hydration enhances activity. The results using gas–phase substrates do not support the existence of a critical hydration value below which enzymatic activity is absent, and suggest that enzyme activity is possible at much lower hydrations than previously thought; they do not support the notion that significant hydration of the surface polar groups is required for activity. However, the marked improvement of activity as hydration is increased suggests that water does play a role, perhaps in optimizing the structure or facilitating the flexibility required for maximal activity.

text: The use of gas–phase substrates to study enzyme catalysis at low hydration Although there are varying estimates as to the degree of enzyme hydration required for activity, a threshold value of ca. 0.2 g of water per gram of protein has been widely accepted. The evidence upon which this is based is reviewed here. In particular, results from the use of gas–phase substrates are discussed. Results using solid–phase enzyme–substrate mixtures are not altogether in accord with those obtained using gas–phase substrates. The use of gaseous substrates and products provides an experimental system in which the hydration of the enzyme can be easily controlled, but which is not limited by diffusion. 
All the results show that increasing hydration enhances activity. The results using gas–phase substrates do not support the existence of a critical hydration value below which enzymatic activity is absent, and suggest that enzyme activity is possible at much lower hydrations than previously thought; they do not support the notion that significant hydration of the surface polar groups is required for activity. However, the marked improvement of activity as hydration is increased suggests that water does play a role, perhaps in optimizing the structure or facilitating the flexibility required for maximal activity.

year: 2004

title: Voluntary disclosure of intellectual capital in Chinese (mainland) companies

authors: An, Yi

subjects: intellectual capital

abstract: This research examines the extent, quality, and determinants of intellectual capital (IC) disclosure in Chinese companies in order to obtain a comprehensive understanding with regard to the current status of IC disclosure in China, and further to provide some recommendations for IC reporting guidelines. A mixed methods approach, combining both qualitative and quantitative elements, was used. Specifically, the research evolved in three stages. 

Firstly, an IC disclosure index was developed as an instrument for content analysis through a questionnaire survey and consultation with a panel of twenty Chinese IC experts. Secondly, two years annual reports of 100 top A-share Chinese firms were coded for data collection using a coding framework developed from the disclosure index. The collected data were then quantified and analyzed so as to determine the extent and quality of IC disclosure by Chinese firms. Finally, a series of hypothesis regarding the correlations between IC disclosure practices of Chinese firms and nine impact factors (or determinants) were deduced on the basis of prior literature and some relevant theories. Then the hypotheses were tested employing the empirical evidence obtained from the second stage through some statistical techniques, such as univariate analysis and multiple regression analysis.

Inconsistent with prior research, the results in this study indicate that the current level of IC disclosure in China was quite high in both extent and quality, and there was no significant information gap between the expectation of Chinese stakeholders and the actual practices of Chinese firms. It is contended that there are three factors motivating Chinese firms to report their IC actively: to reduce information asymmetry between the management of a company and various stakeholder groups; to discharge accountability to various stakeholders; and to signal organizational legitimacy and excellence to the market. 

It was also found that all the impact factors other than ownership structure had a significant effect on the level of IC disclosure of Chinese firms in univariate analysis, while four out of nine factors comprising firm size, ownership concentration, board independence and stand-alone sustainability report had a significant impact on the level of IC disclosure in multiple regression analysis. It is believed that the findings in this research could have a number of implications for academics, investors, managers, regulators and policy makers.

text: Voluntary disclosure of intellectual capital in Chinese (mainland) companies This research examines the extent, quality, and determinants of intellectual capital (IC) disclosure in Chinese companies in order to obtain a comprehensive understanding with regard to the current status of IC disclosure in China, and further to provide some recommendations for IC reporting guidelines. A mixed methods approach, combining both qualitative and quantitative elements, was used. Specifically, the research evolved in three stages. 

Firstly, an IC disclosure index was developed as an instrument for content analysis through a questionnaire survey and consultation with a panel of twenty Chinese IC experts. Secondly, two years annual reports of 100 top A-share Chinese firms were coded for data collection using a coding framework developed from the disclosure index. The collected data were then quantified and analyzed so as to determine the extent and quality of IC disclosure by Chinese firms. Finally, a series of hypothesis regarding the correlations between IC disclosure practices of Chinese firms and nine impact factors (or determinants) were deduced on the basis of prior literature and some relevant theories. Then the hypotheses were tested employing the empirical evidence obtained from the second stage through some statistical techniques, such as univariate analysis and multiple regression analysis.

Inconsistent with prior research, the results in this study indicate that the current level of IC disclosure in China was quite high in both extent and quality, and there was no significant information gap between the expectation of Chinese stakeholders and the actual practices of Chinese firms. It is contended that there are three factors motivating Chinese firms to report their IC actively: to reduce information asymmetry between the management of a company and various stakeholder groups; to discharge accountability to various stakeholders; and to signal organizational legitimacy and excellence to the market. 

It was also found that all the impact factors other than ownership structure had a significant effect on the level of IC disclosure of Chinese firms in univariate analysis, while four out of nine factors comprising firm size, ownership concentration, board independence and stand-alone sustainability report had a significant impact on the level of IC disclosure in multiple regression analysis. It is believed that the findings in this research could have a number of implications for academics, investors, managers, regulators and policy makers.

year: 2012

title: Demystifying a relationship between voluntary work and Māori

authors: Te Momo, Oliver Helena Fiona

abstract: The purpose of this thesis is to provide evidence that critically reviews the relationship between Māori and voluntary work in social service organisations. Since colonisation very little research has been initiated that investigated the involvement of Māori in voluntary activity yet recent statistics show that Māori are the highest participants in this type of work. This thesis provides an analysis of the relationship of Māori to voluntary work and its evolution in a Colonial State, Liberal State, Welfare State, and Neo-Liberal State in New Zealand. Three perspectives of Māori voluntary work provide the foundation for the analysis of this relationship. The first perspective describes voluntary work from a personal experience as an insider. The second perspective explores literature that records Māori involvement in voluntary activity. The third perspective documents life experiences from Māori voluntary workers in New Zealand communities. The three perspectives provide an empirical foundation for the type of relationship that has developed between Māori and voluntary work; Māori and the State; Māori and their place in New Zealand society. My interest in Te Ao Māori (the Māori world) as a valid and thriving ontological position and my academic interest in critical theory provide the theoretical lens for my analysis. 

Personal experience as a Māori volunteer opened doors to the communities I entered and added strength to the research, the first perspective. Western research in Māori communities often by non-Māori researchers, was conducted to gather knowledge for Western policy makers. Māori knowledge was not recognised as valid and the people were treated as objects. Knowing this, I searched for an approach that valued Māori participants, valued Māori people, and contained cultural aspects that separated the procedure from Western research. A review of literature, the second perspective, introduced a cultural approach to research termed kaupapa Māori research, generated out of and for Te Ao Māori detailed further in Chapter Three.

Participants’ life experiences coupled with literature provide a wealth of knowledge and a testimony to the type of relationship that exists between Māori and voluntary work. Criticism from Māori communities as to the exploitation of Māori volunteers has swelled in recent years so that the environment volunteers work in is no longer attractive to Māori people. Therefore, documenting the life experiences of Māori who participate in voluntary work as the primary source of information was imperative and produced evidence to describe the relationship between Māori and voluntary work in the thesis. A triangular study: case study, community study, and cross-section study (Chapters Six, Seven, and Eight), provide the ‘grass roots voices’ and represent the third perspective. 

My observation of Māori organisations’ involvement in voluntary work generated in me a deep concern in what appeared to be an involvement in activity that improved neither the lives of the volunteers nor the wider problems to which they attributed their aroha. This involvement activity is a product of historical developments whereby the Western ideology of voluntary work became confused with a Māori ideology of collective participation. This confusion has created an environment where Māori voluntary organisations are working unpaid with insufficient resources in oppressive conditions in the attempt to provide social solutions. I argue that the Māori ideology of collective participation has been co-opted in the emerging conflict of neo-liberalism to provide social services in communities which government agencies exploit. In conclusion, the thesis is a journey through the world of voluntary work for Māori in communal organisations.

text: Demystifying a relationship between voluntary work and Māori The purpose of this thesis is to provide evidence that critically reviews the relationship between Māori and voluntary work in social service organisations. Since colonisation very little research has been initiated that investigated the involvement of Māori in voluntary activity yet recent statistics show that Māori are the highest participants in this type of work. This thesis provides an analysis of the relationship of Māori to voluntary work and its evolution in a Colonial State, Liberal State, Welfare State, and Neo-Liberal State in New Zealand. Three perspectives of Māori voluntary work provide the foundation for the analysis of this relationship. The first perspective describes voluntary work from a personal experience as an insider. The second perspective explores literature that records Māori involvement in voluntary activity. The third perspective documents life experiences from Māori voluntary workers in New Zealand communities. The three perspectives provide an empirical foundation for the type of relationship that has developed between Māori and voluntary work; Māori and the State; Māori and their place in New Zealand society. My interest in Te Ao Māori (the Māori world) as a valid and thriving ontological position and my academic interest in critical theory provide the theoretical lens for my analysis. 

Personal experience as a Māori volunteer opened doors to the communities I entered and added strength to the research, the first perspective. Western research in Māori communities often by non-Māori researchers, was conducted to gather knowledge for Western policy makers. Māori knowledge was not recognised as valid and the people were treated as objects. Knowing this, I searched for an approach that valued Māori participants, valued Māori people, and contained cultural aspects that separated the procedure from Western research. A review of literature, the second perspective, introduced a cultural approach to research termed kaupapa Māori research, generated out of and for Te Ao Māori detailed further in Chapter Three.

Participants’ life experiences coupled with literature provide a wealth of knowledge and a testimony to the type of relationship that exists between Māori and voluntary work. Criticism from Māori communities as to the exploitation of Māori volunteers has swelled in recent years so that the environment volunteers work in is no longer attractive to Māori people. Therefore, documenting the life experiences of Māori who participate in voluntary work as the primary source of information was imperative and produced evidence to describe the relationship between Māori and voluntary work in the thesis. A triangular study: case study, community study, and cross-section study (Chapters Six, Seven, and Eight), provide the ‘grass roots voices’ and represent the third perspective. 

My observation of Māori organisations’ involvement in voluntary work generated in me a deep concern in what appeared to be an involvement in activity that improved neither the lives of the volunteers nor the wider problems to which they attributed their aroha. This involvement activity is a product of historical developments whereby the Western ideology of voluntary work became confused with a Māori ideology of collective participation. This confusion has created an environment where Māori voluntary organisations are working unpaid with insufficient resources in oppressive conditions in the attempt to provide social solutions. I argue that the Māori ideology of collective participation has been co-opted in the emerging conflict of neo-liberalism to provide social services in communities which government agencies exploit. In conclusion, the thesis is a journey through the world of voluntary work for Māori in communal organisations.

year: 2003