title: The effect of low temperatures on enzyme activity

authors: More, N.

subjects: enzyme

abstract: The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain AN1 and beta-glucosidase from Caldocellum saccharolyticum expressed in Escherichia coli) has been exploited to allow measurement of activity over a 175 degrees C temperature range, from +90 degrees C to -85 degrees C for the glutamate dehydrogenase and from +90 degrees C to -70 degrees C for the beta-glucosidase. The Arrhenius plots of these enzymes, and those for two mesophilic enzymes (glutamate dehydrogenase from bovine liver and beta-galactosidase from Escherichia coli), exhibit no downward deflection corresponding to the glass transition, found by biophysical measurements of several non-enzymic mesophilic proteins at about -65 degrees C and reflecting a sharp decrease in protein flexibility as the overall motion of groups of atoms ceases.

text: The effect of low temperatures on enzyme activity The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain AN1 and beta-glucosidase from Caldocellum saccharolyticum expressed in Escherichia coli) has been exploited to allow measurement of activity over a 175 degrees C temperature range, from +90 degrees C to -85 degrees C for the glutamate dehydrogenase and from +90 degrees C to -70 degrees C for the beta-glucosidase. The Arrhenius plots of these enzymes, and those for two mesophilic enzymes (glutamate dehydrogenase from bovine liver and beta-galactosidase from Escherichia coli), exhibit no downward deflection corresponding to the glass transition, found by biophysical measurements of several non-enzymic mesophilic proteins at about -65 degrees C and reflecting a sharp decrease in protein flexibility as the overall motion of groups of atoms ceases.

year: 1995

title: Studies on the biology of the Catfish: Ictalurus Nebulosus Le Sueur in the Waikato Region

authors: Patchell, Graham James

abstract: This study investigated the age and growth, reproductive and feeding biology of the North American catfish Ictalurus nebulosus Le Sueur in the Waikato Valley of New Zealand, the changes that have occurred during acclimatisation and the extent of the differences in biology between here and other regions of the world. Under Waikato conditions the growth rate of the catfish was much faster than is usual for the species, although the longevity of about 8 years was the same as in the northern hemisphere. Oocyte development in the ovaries was asynchronous (Marza, 1938), with multiple spawning occurring. The duration of the spawning season was nine months, from winter to early autumn. Fecundity estimates were of a minimum of 3700 eggs laid per season. The major foods of the catfish were chironomid larvae, oligochaete worms, molluscs and crustacea. There was no evidence of piscivorous or fish-egg eating habits as has been noted for the species overseas. The catfish is expanding in numbers and range throughout the Waikato region because the recruitment capabilities of the population are high, with a rapid growth rate and high, fecundity, and there is an apparent lack of natural predators.

text: Studies on the biology of the Catfish: Ictalurus Nebulosus Le Sueur in the Waikato Region This study investigated the age and growth, reproductive and feeding biology of the North American catfish Ictalurus nebulosus Le Sueur in the Waikato Valley of New Zealand, the changes that have occurred during acclimatisation and the extent of the differences in biology between here and other regions of the world. Under Waikato conditions the growth rate of the catfish was much faster than is usual for the species, although the longevity of about 8 years was the same as in the northern hemisphere. Oocyte development in the ovaries was asynchronous (Marza, 1938), with multiple spawning occurring. The duration of the spawning season was nine months, from winter to early autumn. Fecundity estimates were of a minimum of 3700 eggs laid per season. The major foods of the catfish were chironomid larvae, oligochaete worms, molluscs and crustacea. There was no evidence of piscivorous or fish-egg eating habits as has been noted for the species overseas. The catfish is expanding in numbers and range throughout the Waikato region because the recruitment capabilities of the population are high, with a rapid growth rate and high, fecundity, and there is an apparent lack of natural predators.

year: 1977

title: Ka Māpuna: Towards a rangatiratanga framework for the governance of waterways

authors: Martin, Betsan

abstract: Rangatiratanga is thenavigational courseforthis research, the aspiration that guides a frameworkfor thegovernance which, in respect ofwaterways and Te Taiao secures mauri, ecosystem health, and water for domestic and commercial use. Rangatiratanga encompasses mana whakahaeredecision-making throughout all relevantgovernance systems. In respect of resource governance, this needs to be achieved through mana whenua authorities and co-governance institutions and procedures.

text: Ka Māpuna: Towards a rangatiratanga framework for the governance of waterways Rangatiratanga is thenavigational courseforthis research, the aspiration that guides a frameworkfor thegovernance which, in respect ofwaterways and Te Taiao secures mauri, ecosystem health, and water for domestic and commercial use. Rangatiratanga encompasses mana whakahaeredecision-making throughout all relevantgovernance systems. In respect of resource governance, this needs to be achieved through mana whenua authorities and co-governance institutions and procedures.

year: 2021