title: Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

authors: Stacheter, Astrid

abstract: The role of soil methylotrophs in methanol exchange with the atmosphere has been widely overlooked. Methanol can be derived from plant polymers and be consumed by soil microbial communities. In the current study, methanol-utilizing methylotrophs of 14 aerated soils were examined to resolve their comparative diversities and capacities to utilize ambient concentrations of methanol. Abundances of cultivable methylotrophs ranged from 10 ⁶-10 ⁸gsoilDW ⁻¹. Methanol dissimilation was measured based on conversion of supplemented 14C-methanol, and occurred at concentrations down to 0.002 μmol methanol gsoilDW ⁻¹. Tested soils exhibited specific affinities to methanol (a ⁰ s=0 ⁻¹) that were similar to those of other environments suggesting that methylotrophs with similar affinities were present. Two deep-branching alphaproteobacterial genotypes of mch responded to the addition of ambient concentrations of methanol (≤0.6 μmol methanol gsoilDW ⁻¹) in one of these soils. Methylotroph community structures were assessed by amplicon pyrosequencing of genes of mono carbon metabolism (mxaF, mch and fae). Alphaproteobacteria-affiliated genotypes were predominant in all investigated soils, and the occurrence of novel genotypes indicated a hitherto unveiled diversity of methylotrophs. Correlations between vegetation type, soil pH and methylotroph community structure suggested that plant-methylotroph interactions were determinative for soil methylotrophs.

text: Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs The role of soil methylotrophs in methanol exchange with the atmosphere has been widely overlooked. Methanol can be derived from plant polymers and be consumed by soil microbial communities. In the current study, methanol-utilizing methylotrophs of 14 aerated soils were examined to resolve their comparative diversities and capacities to utilize ambient concentrations of methanol. Abundances of cultivable methylotrophs ranged from 10 ⁶-10 ⁸gsoilDW ⁻¹. Methanol dissimilation was measured based on conversion of supplemented 14C-methanol, and occurred at concentrations down to 0.002 μmol methanol gsoilDW ⁻¹. Tested soils exhibited specific affinities to methanol (a ⁰ s=0 ⁻¹) that were similar to those of other environments suggesting that methylotrophs with similar affinities were present. Two deep-branching alphaproteobacterial genotypes of mch responded to the addition of ambient concentrations of methanol (≤0.6 μmol methanol gsoilDW ⁻¹) in one of these soils. Methylotroph community structures were assessed by amplicon pyrosequencing of genes of mono carbon metabolism (mxaF, mch and fae). Alphaproteobacteria-affiliated genotypes were predominant in all investigated soils, and the occurrence of novel genotypes indicated a hitherto unveiled diversity of methylotrophs. Correlations between vegetation type, soil pH and methylotroph community structure suggested that plant-methylotroph interactions were determinative for soil methylotrophs.

year: 2012

title: Blind deconvolution of depth-of-field limited full-field lidar data by determination of focal parameters

authors: Godbaz, John Peter

subjects: lidar

abstract: We present a new two-stage method for parametric spatially variant blind deconvolution of full-field Amplitude Modulated Continuous Wave lidar image pairs taken at different aperture settings subject to limited depth of field. A Maximum Likelihood based focal parameter determination algorithm uses range information to reblur the image taken with a smaller aperture size to match the large aperture image. This allows estimation of focal parameters without prior calibration of the optical setup and produces blur estimates which have better spatial resolution and less noise than previous depth from defocus (DFD) blur measurement algorithms. We compare blur estimates from the focal parameter determination method to those from Pentland's DFD method, Subbarao's S-Transform method and estimates from range data/the sampled point spread function. In a second stage the estimated focal parameters are applied to deconvolution of total integrated intensity lidar images improving depth of field. We give an example of application to complex domain lidar images and discuss the trade-off between recovered amplitude texture and sharp range estimates.

text: Blind deconvolution of depth-of-field limited full-field lidar data by determination of focal parameters We present a new two-stage method for parametric spatially variant blind deconvolution of full-field Amplitude Modulated Continuous Wave lidar image pairs taken at different aperture settings subject to limited depth of field. A Maximum Likelihood based focal parameter determination algorithm uses range information to reblur the image taken with a smaller aperture size to match the large aperture image. This allows estimation of focal parameters without prior calibration of the optical setup and produces blur estimates which have better spatial resolution and less noise than previous depth from defocus (DFD) blur measurement algorithms. We compare blur estimates from the focal parameter determination method to those from Pentland's DFD method, Subbarao's S-Transform method and estimates from range data/the sampled point spread function. In a second stage the estimated focal parameters are applied to deconvolution of total integrated intensity lidar images improving depth of field. We give an example of application to complex domain lidar images and discuss the trade-off between recovered amplitude texture and sharp range estimates.

year: 2010

title: The discovery of the Waiorongomai goldfield in 1881

authors: Hart, Philip

abstract: The first reports of gold being found in the vicinity of the Waiorongomai Stream were made in early November 1880, and after the opening of the goldfield a small rush took place to this part of the mountain. The first claims were pegged out in December and the following January, encouraged by some gold being found, but few claims were registered, for most prospectors were quickly disappointed. Prospecting was difficult because of the rugged terrain and heavy bush, and interest quickly faded.
Then, in early October, Hone Werahiko announced his latest and best find and provided samples of payable ore taken from a large reef. Immense excitement ensued, as this was the first payable reef to be found, and after Werahiko led miners on an exhausting trip to his ‘New Find’ a rush set in and many claims were pegged out. The first official to inspect the ground anticipated that the reefs could be easily worked, once an access track was constructed. The small settlement of Te Aroha was revived because a real goldfield had been found, at last.

text: The discovery of the Waiorongomai goldfield in 1881 The first reports of gold being found in the vicinity of the Waiorongomai Stream were made in early November 1880, and after the opening of the goldfield a small rush took place to this part of the mountain. The first claims were pegged out in December and the following January, encouraged by some gold being found, but few claims were registered, for most prospectors were quickly disappointed. Prospecting was difficult because of the rugged terrain and heavy bush, and interest quickly faded.
Then, in early October, Hone Werahiko announced his latest and best find and provided samples of payable ore taken from a large reef. Immense excitement ensued, as this was the first payable reef to be found, and after Werahiko led miners on an exhausting trip to his ‘New Find’ a rush set in and many claims were pegged out. The first official to inspect the ground anticipated that the reefs could be easily worked, once an access track was constructed. The small settlement of Te Aroha was revived because a real goldfield had been found, at last.

year: 2016