title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

authors: Jacobsen, Carsten Suhr

subjects: Science & Technology

abstract: Here we show that a commercial blocking reagent (G2) based on modifed eukaryotic DNA signifcantly improved DNA extraction efciency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the number of PCR-amplifed16S rRNA genes recovered from biomass naturally present in the soils by one log unit. An extensive sequencing approach demonstrated that the blocking reagent was free of contaminating DNA, and may therefore also be used in metagenomics studies that require direct sequencing.

text: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples Here we show that a commercial blocking reagent (G2) based on modifed eukaryotic DNA signifcantly improved DNA extraction efciency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the number of PCR-amplifed16S rRNA genes recovered from biomass naturally present in the soils by one log unit. An extensive sequencing approach demonstrated that the blocking reagent was free of contaminating DNA, and may therefore also be used in metagenomics studies that require direct sequencing.

year: 2018-04-09

title: Whatever next? Export market choices of New Zealand firms

authors: Fabling, Richard

subjects: trade

abstract: We examine product and market entry choices of New Zealand exporters, using enterprise-level data on firm performance and merchandise trade. For firms already exporting, we ask: What determines their choices about what and where to export? General and specific prior trade experience play an important role in determining firms' future export activities, as do the export activities of other firms in the local area (a learning effect). These results are robust to the inclusion of other export determinants, including the macroeconomic performance of destination countries, exchange rate movements, and the past performance of the exporting firm.

text: Whatever next? Export market choices of New Zealand firms We examine product and market entry choices of New Zealand exporters, using enterprise-level data on firm performance and merchandise trade. For firms already exporting, we ask: What determines their choices about what and where to export? General and specific prior trade experience play an important role in determining firms' future export activities, as do the export activities of other firms in the local area (a learning effect). These results are robust to the inclusion of other export determinants, including the macroeconomic performance of destination countries, exchange rate movements, and the past performance of the exporting firm.

year: 2011

title: Enacting Kaitiakitanga Challenges and Complexities in the Governance and Ownership of Rongoā Research Information.

authors: Boulton, Amohia

subjects: Indigenous

abstract: This article explores the tensions one research team has faced in securing appropriate governance or stewardship (which we refer to as kaitiakitanga) of research data. Whilst ethical and regulatory frameworks exist which provide a minimum standard for researchers to meet when working with Māori, what our experience has highlighted is there is currently a “governance” gap in terms of who should hold stewardship of research data collected from Māori individuals or collectives. In the case of a project undertaken in the traditional healing space, the organisation best placed to fulfil this governance role receives no funding or support to take on such a responsibility; consequently by default, this role is being borne by the research team until such time as capacity can be built and adequate resourcing secured. In addition, we have realised that the tensions played out in this research project have implications for the broader issue of how we protect traditional knowledge in a modern intellectual property law context, and once again how we adequately support those, often community-based organisations, who work at the interface between Indigenous knowledge and the Western world.

text: Enacting Kaitiakitanga Challenges and Complexities in the Governance and Ownership of Rongoā Research Information. This article explores the tensions one research team has faced in securing appropriate governance or stewardship (which we refer to as kaitiakitanga) of research data. Whilst ethical and regulatory frameworks exist which provide a minimum standard for researchers to meet when working with Māori, what our experience has highlighted is there is currently a “governance” gap in terms of who should hold stewardship of research data collected from Māori individuals or collectives. In the case of a project undertaken in the traditional healing space, the organisation best placed to fulfil this governance role receives no funding or support to take on such a responsibility; consequently by default, this role is being borne by the research team until such time as capacity can be built and adequate resourcing secured. In addition, we have realised that the tensions played out in this research project have implications for the broader issue of how we protect traditional knowledge in a modern intellectual property law context, and once again how we adequately support those, often community-based organisations, who work at the interface between Indigenous knowledge and the Western world.

year: 2014