title: Enzyme activity and flexibility at very low hydration

authors: Kurkal, Vandana

abstract: Recent measurements have demonstrated enzyme activity at hydrations as low as 3%. This raises the question of whether hydration-induced enzyme flexibility is important for activity. Here, to address this, picosecond dynamic neutron scattering experiments are performed on pig liver esterase powders at 0%, 3%, 12%, and 50% hydration by weight and at temperatures ranging from 120 to 300 K. At all temperatures and hydrations, significant quasielastic scattering intensity is found in the protein, indicating the presence of anharmonic, diffusive motion. As the hydration increases, a temperature-dependent dynamical transition appears and strengthens involving additional diffusive motion. The implication of these results is that, although the additional hydration-induced diffusive motion in the protein detected here may be related to increased activity, it is not required for the enzyme to function.

text: Enzyme activity and flexibility at very low hydration Recent measurements have demonstrated enzyme activity at hydrations as low as 3%. This raises the question of whether hydration-induced enzyme flexibility is important for activity. Here, to address this, picosecond dynamic neutron scattering experiments are performed on pig liver esterase powders at 0%, 3%, 12%, and 50% hydration by weight and at temperatures ranging from 120 to 300 K. At all temperatures and hydrations, significant quasielastic scattering intensity is found in the protein, indicating the presence of anharmonic, diffusive motion. As the hydration increases, a temperature-dependent dynamical transition appears and strengthens involving additional diffusive motion. The implication of these results is that, although the additional hydration-induced diffusive motion in the protein detected here may be related to increased activity, it is not required for the enzyme to function.

year: 2005-05

title: From charity to 'not-for-profit': Changes in the role and structure of voluntary social service agencies

authors: Barnes, Jo

subjects: New Zealand

abstract: This chapter discusses findings from a study undertaken in 2001-02 on not-for-profit social service organisations in the regional city of Hamilton, a city of 166,000 people, in the context of changes in New Zealand welfare governance from 1984 to 1999 and inTampa, Florida, a city of just over 300,000 people, following the US welfare reforms of the Clinton administration.

text: From charity to 'not-for-profit': Changes in the role and structure of voluntary social service agencies This chapter discusses findings from a study undertaken in 2001-02 on not-for-profit social service organisations in the regional city of Hamilton, a city of 166,000 people, in the context of changes in New Zealand welfare governance from 1984 to 1999 and inTampa, Florida, a city of just over 300,000 people, following the US welfare reforms of the Clinton administration.

year: 2006

title: Whiti te rā: A guide to connecting Māori to traditional wellbeing pathways.

authors: McLachlan, Andre David

abstract: Māori health models, introduced in the 1980s, brought needed cultural worldviews to an otherwise monocultural health system. However, minimal changes have occurred. In mainstream practice, deeper cultural understandings and action-orientations of these models can be overlooked, to the detriment of Māori wellbeing.
In particular, Māori cultural concepts such as mauri ora (an active state of wellbeing) and a secure cultural identity are notable core wellbeing pathways that need further exploration. Using a systematic narrative literature review, 36 papers identified pathways that used core cultural activities for Māori wellbeing. A thematic analysis produced six themes or pathways towards wellbeing for Māori – te reo Māori: Māori language, taiao: connection with the environment, wairua: Māori spiritual beliefs and practices, mahi-a-toi:
Māori expressive art forms, take pū whānau: Māori relational values, and whakapapa: intergenerational relationships. Forty experienced Māori psychologists analysed the themes and offered expert examples practice pathways for Māori wellbeing. The six themes and feedback is presented in a visual image Whiti Te Rā with instructions for Māori practitioners to guide whai ora Māori (Māori clients) to explore their level of knowledge and comfort, and active engagement with Māori pathways to wellbeing. The model has potential for mental health policy, future research, curriculum development, and synthesising Māori knowledge towards wellbeing pathways.

text: Whiti te rā: A guide to connecting Māori to traditional wellbeing pathways. Māori health models, introduced in the 1980s, brought needed cultural worldviews to an otherwise monocultural health system. However, minimal changes have occurred. In mainstream practice, deeper cultural understandings and action-orientations of these models can be overlooked, to the detriment of Māori wellbeing.
In particular, Māori cultural concepts such as mauri ora (an active state of wellbeing) and a secure cultural identity are notable core wellbeing pathways that need further exploration. Using a systematic narrative literature review, 36 papers identified pathways that used core cultural activities for Māori wellbeing. A thematic analysis produced six themes or pathways towards wellbeing for Māori – te reo Māori: Māori language, taiao: connection with the environment, wairua: Māori spiritual beliefs and practices, mahi-a-toi:
Māori expressive art forms, take pū whānau: Māori relational values, and whakapapa: intergenerational relationships. Forty experienced Māori psychologists analysed the themes and offered expert examples practice pathways for Māori wellbeing. The six themes and feedback is presented in a visual image Whiti Te Rā with instructions for Māori practitioners to guide whai ora Māori (Māori clients) to explore their level of knowledge and comfort, and active engagement with Māori pathways to wellbeing. The model has potential for mental health policy, future research, curriculum development, and synthesising Māori knowledge towards wellbeing pathways.

year: 2021