title: Stable isotope and molecular analyses indicate that hybridization with non-native domesticated common carp influence habitat use of native carp

authors: Matsuzaki, Shin-ichiro S.

subjects: non-native species

abstract: Hybridization between native and non-native species has consequences for survival and growth rates of hybrid offspring, but the influences on their functional roles such as habitat use are little studied and poorly understood. The Japanese native common carp Cyprinus carpio coexist and hybridize with non-native domesticated carp in natural Japanese lakes. We have combined stable isotope and molecular information to examine whether habitat use of carp varies depending on the degree of hybridization between native and non-native carp. We sampled 69 carp from Lake Kasumigaura where hybrid swarms between native and non-native carp are advancing, evaluated the degree of hybridization for each individual by genotyping five single nucleotide polymorphism (SNP) markers, and analyzed their carbon and nitrogen stable isotopes. Although we did not find any genetically pure native carp in the lake, the results showed that carp δ13C increased with increasing frequency of non-native alleles but that δ15N did not change. This indicates that non-native carp use the littoral zone more frequently than native carp. This difference in habitat use was supported by a multisource mixing model, showing that the contribution of limnetic primary consumers to the diets of non-native carp was lower than that of individuals with the highest frequency of native alleles. By combining two very different methods, our results thus suggest that multiple-generation hybridization can influence habitat and resource use. Habitat partitioning should be considered when evaluating the genetic impacts of invasive species and races on native species and ecosystem processes.

text: Stable isotope and molecular analyses indicate that hybridization with non-native domesticated common carp influence habitat use of native carp Hybridization between native and non-native species has consequences for survival and growth rates of hybrid offspring, but the influences on their functional roles such as habitat use are little studied and poorly understood. The Japanese native common carp Cyprinus carpio coexist and hybridize with non-native domesticated carp in natural Japanese lakes. We have combined stable isotope and molecular information to examine whether habitat use of carp varies depending on the degree of hybridization between native and non-native carp. We sampled 69 carp from Lake Kasumigaura where hybrid swarms between native and non-native carp are advancing, evaluated the degree of hybridization for each individual by genotyping five single nucleotide polymorphism (SNP) markers, and analyzed their carbon and nitrogen stable isotopes. Although we did not find any genetically pure native carp in the lake, the results showed that carp δ13C increased with increasing frequency of non-native alleles but that δ15N did not change. This indicates that non-native carp use the littoral zone more frequently than native carp. This difference in habitat use was supported by a multisource mixing model, showing that the contribution of limnetic primary consumers to the diets of non-native carp was lower than that of individuals with the highest frequency of native alleles. By combining two very different methods, our results thus suggest that multiple-generation hybridization can influence habitat and resource use. Habitat partitioning should be considered when evaluating the genetic impacts of invasive species and races on native species and ecosystem processes.

year: 2010

title: Scamper: a scalable and extensible packet prober for active measurement of the internet

authors: Luckie, Matthew John

subjects: computer science

abstract: Path MTU Discovery (PMTUD) is widely believed to be unreliable because of firewalls that discard ICMP “Packet Too Big”messages. This paper measures PMTUD behaviour for 50,000 popular websites and finds the failure rate in IPv4 is much less than previous studies. We measure the overall failure rate between 5% and 18%, depending on the MTU of the constraining link. We explore methods webserver operators are using to reduce their dependence on PMTUD, and find 11% limit themselves to sending packets no larger than 1380 bytes. We identify a number of common behaviours that seem to be software bugs rather than filtering by firewalls. If these are corrected PMTUD failures could be reduced by 63%. We further find the IPv6 failure rate is less than the IPv4 rate even with more scope for failure in IPv6.

text: Scamper: a scalable and extensible packet prober for active measurement of the internet Path MTU Discovery (PMTUD) is widely believed to be unreliable because of firewalls that discard ICMP “Packet Too Big”messages. This paper measures PMTUD behaviour for 50,000 popular websites and finds the failure rate in IPv4 is much less than previous studies. We measure the overall failure rate between 5% and 18%, depending on the MTU of the constraining link. We explore methods webserver operators are using to reduce their dependence on PMTUD, and find 11% limit themselves to sending packets no larger than 1380 bytes. We identify a number of common behaviours that seem to be software bugs rather than filtering by firewalls. If these are corrected PMTUD failures could be reduced by 63%. We further find the IPv6 failure rate is less than the IPv4 rate even with more scope for failure in IPv6.

year: 2010

title: Kaupapa Māori antenatal wānanga: A solution to refocus New Zealand health service delivery for improved engagement and responsiveness with Māori māmā hapū and pēpi.

authors: Barrett, Nikki Maree

subjects: antenatal

abstract: The revitalisation of traditional Māori birthing knowledge and practices is a significant space to enhance Māori maternal and infant health outcomes. This thesis with publications examined the potential of two Kaupapa Māori antenatal wānanga to enhance maternal health outcomes for Māori. Using a mixed methods approach, underpinned by Kaupapa Māori research principles and supported by Māori centred research and a mana wahine theoretical perspective, this thesis reveals transformative solutions to address maternal and infant inequities. 
This thesis explores three key areas. First, maternal and infant education and knowledge during hapūtanga (pregnancy). Second, the connection and engagement of Māori māmā hapū (expectant mothers) and whānau (family) with health and community services, and third, the lived experiences of Māori māmā engaging with community services to identify barriers and enablers of service providers.  
The introductory chapters provide a contextual overview of maternal and infant health. I begin by canvassing traditional and contemporary factors that influence the current state of Indigenous, notably Māori, maternal and infant health. I then present four chapters that have been submitted to academic journals for publication. Each chapter focuses on one or more of the three key areas identified above. The final chapter draws each of the chapters together to demonstrate how Māori led health initiatives work to enhance and improve maternal health .
Chapter 3 provides a rationale for focusing on Indigenous antenatal education. Within Western countries, antenatal education is a recognised and encouraged service provided during pregnancy. Providing education and information, antenatal education is an opportunity for expectant parents to prepare for pregnancy and parenting. With poor maternal and infant health outcomes, Indigenous Peoples from Aotearoa, Australia, Canada, and the United States, are particularly encouraged to attend antenatal education. Yet, the low rates of Indigenous Peoples attending antenatal education (will be interchangeably referred to as childbirth education throughout this thesis) do not reflect this need. Therefore, the first process in this thesis was to explore Indigenous Peoples participation and engagement in antenatal education. Using a standardised protocol, chapter 3 is a systematic review that explored how Indigenous Peoples are involved, prioritised, and privileged in antenatal education. Specifically, this systematic review examined the extent to which Indigenous Peoples, and their aspirations of health, were prioritised in academic scholarship. With only two studies involving Indigenous participants, it was evidenced that the voice of Indigenous Peoples in antenatal education is lacking in academic scholarship. A key finding was that Indigenous antenatal education programmes were not reflected in academic scholarship. Yet, within Aotearoa there are existing Kaupapa Māori antenatal education. Therefore, the next chapter (4) focused on a retrospective audit of Hapū Wānanga, a Kaupapa Māori childbirth education class.  
One reason for undertaking a retrospective audit of Hapū Wānanga (HW) was because I was genuinely interested to understand how other māmā hapū, and their whānau, experienced the programme. I wanted to give voice to these wāhine and was excited to learn whether the programme had similar value to them, as it did for me when I participated in a similar wānanga. This mixed-method interpretive study used retrospective post-course survey data of 1,152 participants over a three-year period from the HW based in the Waikato District Health Board region. This chapter amplified the experiences and voices of HW participants to affirm positive aspects of the class. Participants regarded the information that was shared as life changing, describing the constituents that separated this programme from other health service experiences. Findings revealed that an Indigenous, strengths-based approach to childbirth education service design and delivery, has positive and transformation results for whānau.
Chapter 5 focused on supporting another Kaupapa Māori antenatal wānanga, Whirihia Te Korowai Aroha, to co-design a holistic assessment tool (Whirihia tool). The Whirihia tool aimed to increase access and enhance experiences between health services and māmā hapū by identifying health or social need, then providing a dedicated referral pathway. Using the Indigenous He Pikinga Waiora Implementation (HPW) framework as a guide, this chapter provides an autoethnographic reflective account of the co-design process that led to the development and implementation of the Whirihia tool. This reflective account provides examples of key considerations that align to the HPW framework in the hopes that it will afford some guidance for fellow emerging researchers who wish to undertake ethical co-designed health research with Māori (and non-Māori) communities and organisations. The intention of this chapter is to encourage our research community to provide opportunities and platforms for community health initiatives such as Whirihia Te Korowai Aroha to be foregrounded in academic scholarship. Kaupapa Māori programmes such as these have the solutions to address issues that predominantly affect Māori, however they need the vehicles and tools to make Māori voices, visibility, and vision, heard.   
A cohort study was used to extend on the findings from the autoethnographic co-designed Whirihia tool. This chapter explored the experiences of māmā hapū and their interactions with health and social services immediately after Aotearoa’s first Covid-19 lockdown in March 2020. 17 participants completed the Whirihia tool with a subsequent seven māmā participating in a semi-structured, open-ended telephone interview. Findings showed that when services enabled participants to enact their autonomy and self-determination participants valued their engagement with that service. To be responsive to Māori health needs and aspirations, services must demonstrate empathy and compassion for māmā and operate in a passive role of support rather than a position of authority and power.
The final chapter draws together the findings from the previous chapters and challenges preconceived notions that Māori do not engage with maternal and infant health services. Instead, this thesis demonstrates that Māori-led, designed, and delivered, initiatives attract Māori māmā hapū, wider whānau, and non-Māori engagement and endorsement. Foregrounded by the voice and experiences of Māori māmā hapū and their whānau, I suggest three practical steps that can be enacted immediately at both a health system, and health service delivery level to positively enhance antenatal health. First, determine the extent to which CBE classes are meeting Māori end-user needs. Second, using findings from end-user experiences as a foundation, reorient the Ministry of Health pregnancy and parenting service specifications to ensure appropriate content is prioritised in CBE classes, particularly Māori knowledge. Third, adequately resource CBE providers who can deliver programmes that meet the needs of Māori end-users. With the new Te Whatu Ora Health New Zealand and Te Aka Whai Ora Māori Health Authority, now is the time to deliver on the various strategic documents to authentically meet the needs of māmā hapū and whānau. 
The evidence-based research delivered in this thesis provided the basis for the three recommended changes noted above. These recommendations can be implemented into the health system to enable positive and meaningful change for Māori, in addition to supporting efforts of other Indigenous Peoples to improve Indigenous perinatal health and wellbeing.

text: Kaupapa Māori antenatal wānanga: A solution to refocus New Zealand health service delivery for improved engagement and responsiveness with Māori māmā hapū and pēpi. The revitalisation of traditional Māori birthing knowledge and practices is a significant space to enhance Māori maternal and infant health outcomes. This thesis with publications examined the potential of two Kaupapa Māori antenatal wānanga to enhance maternal health outcomes for Māori. Using a mixed methods approach, underpinned by Kaupapa Māori research principles and supported by Māori centred research and a mana wahine theoretical perspective, this thesis reveals transformative solutions to address maternal and infant inequities. 
This thesis explores three key areas. First, maternal and infant education and knowledge during hapūtanga (pregnancy). Second, the connection and engagement of Māori māmā hapū (expectant mothers) and whānau (family) with health and community services, and third, the lived experiences of Māori māmā engaging with community services to identify barriers and enablers of service providers.  
The introductory chapters provide a contextual overview of maternal and infant health. I begin by canvassing traditional and contemporary factors that influence the current state of Indigenous, notably Māori, maternal and infant health. I then present four chapters that have been submitted to academic journals for publication. Each chapter focuses on one or more of the three key areas identified above. The final chapter draws each of the chapters together to demonstrate how Māori led health initiatives work to enhance and improve maternal health .
Chapter 3 provides a rationale for focusing on Indigenous antenatal education. Within Western countries, antenatal education is a recognised and encouraged service provided during pregnancy. Providing education and information, antenatal education is an opportunity for expectant parents to prepare for pregnancy and parenting. With poor maternal and infant health outcomes, Indigenous Peoples from Aotearoa, Australia, Canada, and the United States, are particularly encouraged to attend antenatal education. Yet, the low rates of Indigenous Peoples attending antenatal education (will be interchangeably referred to as childbirth education throughout this thesis) do not reflect this need. Therefore, the first process in this thesis was to explore Indigenous Peoples participation and engagement in antenatal education. Using a standardised protocol, chapter 3 is a systematic review that explored how Indigenous Peoples are involved, prioritised, and privileged in antenatal education. Specifically, this systematic review examined the extent to which Indigenous Peoples, and their aspirations of health, were prioritised in academic scholarship. With only two studies involving Indigenous participants, it was evidenced that the voice of Indigenous Peoples in antenatal education is lacking in academic scholarship. A key finding was that Indigenous antenatal education programmes were not reflected in academic scholarship. Yet, within Aotearoa there are existing Kaupapa Māori antenatal education. Therefore, the next chapter (4) focused on a retrospective audit of Hapū Wānanga, a Kaupapa Māori childbirth education class.  
One reason for undertaking a retrospective audit of Hapū Wānanga (HW) was because I was genuinely interested to understand how other māmā hapū, and their whānau, experienced the programme. I wanted to give voice to these wāhine and was excited to learn whether the programme had similar value to them, as it did for me when I participated in a similar wānanga. This mixed-method interpretive study used retrospective post-course survey data of 1,152 participants over a three-year period from the HW based in the Waikato District Health Board region. This chapter amplified the experiences and voices of HW participants to affirm positive aspects of the class. Participants regarded the information that was shared as life changing, describing the constituents that separated this programme from other health service experiences. Findings revealed that an Indigenous, strengths-based approach to childbirth education service design and delivery, has positive and transformation results for whānau.
Chapter 5 focused on supporting another Kaupapa Māori antenatal wānanga, Whirihia Te Korowai Aroha, to co-design a holistic assessment tool (Whirihia tool). The Whirihia tool aimed to increase access and enhance experiences between health services and māmā hapū by identifying health or social need, then providing a dedicated referral pathway. Using the Indigenous He Pikinga Waiora Implementation (HPW) framework as a guide, this chapter provides an autoethnographic reflective account of the co-design process that led to the development and implementation of the Whirihia tool. This reflective account provides examples of key considerations that align to the HPW framework in the hopes that it will afford some guidance for fellow emerging researchers who wish to undertake ethical co-designed health research with Māori (and non-Māori) communities and organisations. The intention of this chapter is to encourage our research community to provide opportunities and platforms for community health initiatives such as Whirihia Te Korowai Aroha to be foregrounded in academic scholarship. Kaupapa Māori programmes such as these have the solutions to address issues that predominantly affect Māori, however they need the vehicles and tools to make Māori voices, visibility, and vision, heard.   
A cohort study was used to extend on the findings from the autoethnographic co-designed Whirihia tool. This chapter explored the experiences of māmā hapū and their interactions with health and social services immediately after Aotearoa’s first Covid-19 lockdown in March 2020. 17 participants completed the Whirihia tool with a subsequent seven māmā participating in a semi-structured, open-ended telephone interview. Findings showed that when services enabled participants to enact their autonomy and self-determination participants valued their engagement with that service. To be responsive to Māori health needs and aspirations, services must demonstrate empathy and compassion for māmā and operate in a passive role of support rather than a position of authority and power.
The final chapter draws together the findings from the previous chapters and challenges preconceived notions that Māori do not engage with maternal and infant health services. Instead, this thesis demonstrates that Māori-led, designed, and delivered, initiatives attract Māori māmā hapū, wider whānau, and non-Māori engagement and endorsement. Foregrounded by the voice and experiences of Māori māmā hapū and their whānau, I suggest three practical steps that can be enacted immediately at both a health system, and health service delivery level to positively enhance antenatal health. First, determine the extent to which CBE classes are meeting Māori end-user needs. Second, using findings from end-user experiences as a foundation, reorient the Ministry of Health pregnancy and parenting service specifications to ensure appropriate content is prioritised in CBE classes, particularly Māori knowledge. Third, adequately resource CBE providers who can deliver programmes that meet the needs of Māori end-users. With the new Te Whatu Ora Health New Zealand and Te Aka Whai Ora Māori Health Authority, now is the time to deliver on the various strategic documents to authentically meet the needs of māmā hapū and whānau. 
The evidence-based research delivered in this thesis provided the basis for the three recommended changes noted above. These recommendations can be implemented into the health system to enable positive and meaningful change for Māori, in addition to supporting efforts of other Indigenous Peoples to improve Indigenous perinatal health and wellbeing.

year: 2023