title: Identifying diazotrophs by incorporation of nitrogen from ¹⁵N₂ into RNA

authors: Addison, Sarah Louise

subjects: nitrogen fixing

abstract: The diversity and abundance of active diazotrophs was investigated in a New Zealand pulp and paper wastewater by enrichment with ¹⁵N₂. Purified ¹⁵N-RNA was analysed by reverse transcription, molecular cloning and sequence analysis of 16S rRNA to reveal a diverse community of bacteria as indicated by a Shannon Weaver Index value of > 2.8. The major class represented in the enriched culture were the γ-Proteobacteria at 85% with a secondary group of the phylum Firmicutes present at 8.2%, the remaining sequences were affiliated with the α- and β-Proteobacterial classes (1.4% and 4.3%, respectively). Three dominant genera, Aeromonas, Pseudomonas and Bacillus, were identified by comparison with published sequences and phylogenetic analysis. To confirm that representatives of the taxonomic groups identified from the active enriched nitrogen-fixing community were capable of fixing nitrogen Aeromonas and Pseudomonas species were cultivated and shown to possess nifH genes. In wastewater, fluorescence in situ hybridisation probing revealed that the dominant nitrogen-fixing population identified in this study were present in the population, but at lower levels. The population is, therefore, reliant on a small sub-population of diazotrophs to supply the community's nitrogen needs above that already present in the wastewater.

text: Identifying diazotrophs by incorporation of nitrogen from ¹⁵N₂ into RNA The diversity and abundance of active diazotrophs was investigated in a New Zealand pulp and paper wastewater by enrichment with ¹⁵N₂. Purified ¹⁵N-RNA was analysed by reverse transcription, molecular cloning and sequence analysis of 16S rRNA to reveal a diverse community of bacteria as indicated by a Shannon Weaver Index value of > 2.8. The major class represented in the enriched culture were the γ-Proteobacteria at 85% with a secondary group of the phylum Firmicutes present at 8.2%, the remaining sequences were affiliated with the α- and β-Proteobacterial classes (1.4% and 4.3%, respectively). Three dominant genera, Aeromonas, Pseudomonas and Bacillus, were identified by comparison with published sequences and phylogenetic analysis. To confirm that representatives of the taxonomic groups identified from the active enriched nitrogen-fixing community were capable of fixing nitrogen Aeromonas and Pseudomonas species were cultivated and shown to possess nifH genes. In wastewater, fluorescence in situ hybridisation probing revealed that the dominant nitrogen-fixing population identified in this study were present in the population, but at lower levels. The population is, therefore, reliant on a small sub-population of diazotrophs to supply the community's nitrogen needs above that already present in the wastewater.

year: 2010

title: Oncology Nurses' Knowledge of Survivorship Care Planning: The Need for Education

authors: Lester, Joanne L.

subjects: late effects of cancer treatment

abstract: Purpose/Objectives: To survey nurses about their knowledge of cancer survivorship care.

Design: Descriptive, cross-sectional.

Setting: Midwestern comprehensive cancer center.

Sample: 223 registered and advanced practice nurses.

Methods: Online survey of survivorship knowledge using a 50-item questionnaire derived from the Institute of Medicine report and related publications.

Main Research Variables: Concepts of survivorship care and common long-term symptoms.

Findings: Most nurses reported having knowledge about healthy lifestyle habits; more than 50% of nurses reported having knowledge about chemotherapy, surgery, and radiation therapy, as well as side effects of fatigue, depression, limitations of daily activities, and weight gain; less than 50% of nurses reported having knowledge of impact on family, biologic agents, lymphedema, immunizations or vaccinations, and osteoporosis screening; less than 40% of nurses reported having knowledge about marital and partner relationships, osteoporosis prevention and care, sexuality, side effects of bone marrow transplantation, employment issues, and angiogenesis agents; and less than 25% of nurses reported having knowledge on genetic risks, as well as fertility, financial, and insurance issues.

Conclusions: Oncology nurses at an academic comprehensive cancer center reported gaps in knowledge consistent with previous studies about knowledge of survivorship care.

Implications for Nursing: The Institute of Medicine has challenged oncology providers to address cancer survivorship care planning. Gaps in cancer survivorship knowledge are evident and will require focused education for this initiative to be successful.

text: Oncology Nurses' Knowledge of Survivorship Care Planning: The Need for Education Purpose/Objectives: To survey nurses about their knowledge of cancer survivorship care.

Design: Descriptive, cross-sectional.

Setting: Midwestern comprehensive cancer center.

Sample: 223 registered and advanced practice nurses.

Methods: Online survey of survivorship knowledge using a 50-item questionnaire derived from the Institute of Medicine report and related publications.

Main Research Variables: Concepts of survivorship care and common long-term symptoms.

Findings: Most nurses reported having knowledge about healthy lifestyle habits; more than 50% of nurses reported having knowledge about chemotherapy, surgery, and radiation therapy, as well as side effects of fatigue, depression, limitations of daily activities, and weight gain; less than 50% of nurses reported having knowledge of impact on family, biologic agents, lymphedema, immunizations or vaccinations, and osteoporosis screening; less than 40% of nurses reported having knowledge about marital and partner relationships, osteoporosis prevention and care, sexuality, side effects of bone marrow transplantation, employment issues, and angiogenesis agents; and less than 25% of nurses reported having knowledge on genetic risks, as well as fertility, financial, and insurance issues.

Conclusions: Oncology nurses at an academic comprehensive cancer center reported gaps in knowledge consistent with previous studies about knowledge of survivorship care.

Implications for Nursing: The Institute of Medicine has challenged oncology providers to address cancer survivorship care planning. Gaps in cancer survivorship knowledge are evident and will require focused education for this initiative to be successful.

year: 2014-02-27

title: Ideology is theft: Thoughts on the legitimacy of a Maori psychology

authors: Tamatea, Armon J.

subjects: Maori

abstract: ‘War, in fact, can be seen as a process of achieving equilibrium among unequal technologies’ (McLuhan, 1964) 
We are at war. As Western science and its accompanying technology expands the frontiers of knowledge at an ever-increasing rate, ‘indigenous’ perspectives of knowledge are exiled into the borderlands of special interest groups and localized research programmes. Mainstream scientific thought lays claim to objective interpretations of experience at the expense of alternative realities offered by emerging theories of knowledge. Furthermore, as localized worldviews (i.e., those derived from ancestral knowledge bases and pre-industrial or non-scientific premises) challenge existing paradigms, the inevitable interactions threaten to undermine the fidelity of this knowledge. One such arena where this ideological conflict is apparent is the growing field of Maori psychology.

text: Ideology is theft: Thoughts on the legitimacy of a Maori psychology ‘War, in fact, can be seen as a process of achieving equilibrium among unequal technologies’ (McLuhan, 1964) 
We are at war. As Western science and its accompanying technology expands the frontiers of knowledge at an ever-increasing rate, ‘indigenous’ perspectives of knowledge are exiled into the borderlands of special interest groups and localized research programmes. Mainstream scientific thought lays claim to objective interpretations of experience at the expense of alternative realities offered by emerging theories of knowledge. Furthermore, as localized worldviews (i.e., those derived from ancestral knowledge bases and pre-industrial or non-scientific premises) challenge existing paradigms, the inevitable interactions threaten to undermine the fidelity of this knowledge. One such arena where this ideological conflict is apparent is the growing field of Maori psychology.

year: 2008