title: How to survive a plague of flesh-eating rats: An introductory guide to studying remediated ‎gameplay imaginations of medieval folklore and beliefs in A Plague Tale: Innocence

authors: Schott, Gareth R.

abstract: Popular digital games such as Assassin’s Creed: Origins (2017), Total War: Three Kingdoms (2019), and Red: Dead Redemption (2010) possess merit for their faithful and authentic interactive representations of elements of the past that furnish games with pedagogical value. Both digital game studies and historical game studies have contributed research furthering understanding of the pedagogical value and applications of historical games for students’ learning of history in school education, particularly secondary schools and universities (Kee, 2014, McCall, 2013, &amp; Schrier, 2014). Despite the growing interest in the new forms of knowledge contained within games, and gameplay, the educational application of historical digital games can be limited to supplementary visual aids that do not fully account for the range and forms of historical investigation associated with the game development process. We forward a multi-modal perspective on the varied ways in which historical knowledge is present in both game design and the gameplay experience. Our approach is demonstrated with one of several under-investigated (yet valuable) modes of historical exploration, namely ‘Imaginative History.’ This is achieved using images from several sequences of recorded game footage in the Medieval historical fantasy game A Plague Tale: Innocence as a case example alongside supporting historical literature. This Medieval game is suitable for addressing this conference’s theme of crises or disasters as it is set in a re-imagination of the Black Death plague roughly within the south-western region of fourteenth century France, but one that personifies the real Black Death with a plague of supernatural flesh-eating rats and places players into the role of two fictional orphan children. This presentation seeks to demonstrate historical games’ value for teaching and learning by discussing A Plague Tale: Innocence’s fantastical adaptation of the plague as a model for exploring the role of re-mediation, and subversion, of past pre-modern folklore imaginations and beliefs. Fantasy or folkloric treatment of historical events or periods can provide insight into the experiential dimensions of a period in history re-told as an interactive folktale.

text: How to survive a plague of flesh-eating rats: An introductory guide to studying remediated ‎gameplay imaginations of medieval folklore and beliefs in A Plague Tale: Innocence Popular digital games such as Assassin’s Creed: Origins (2017), Total War: Three Kingdoms (2019), and Red: Dead Redemption (2010) possess merit for their faithful and authentic interactive representations of elements of the past that furnish games with pedagogical value. Both digital game studies and historical game studies have contributed research furthering understanding of the pedagogical value and applications of historical games for students’ learning of history in school education, particularly secondary schools and universities (Kee, 2014, McCall, 2013, &amp; Schrier, 2014). Despite the growing interest in the new forms of knowledge contained within games, and gameplay, the educational application of historical digital games can be limited to supplementary visual aids that do not fully account for the range and forms of historical investigation associated with the game development process. We forward a multi-modal perspective on the varied ways in which historical knowledge is present in both game design and the gameplay experience. Our approach is demonstrated with one of several under-investigated (yet valuable) modes of historical exploration, namely ‘Imaginative History.’ This is achieved using images from several sequences of recorded game footage in the Medieval historical fantasy game A Plague Tale: Innocence as a case example alongside supporting historical literature. This Medieval game is suitable for addressing this conference’s theme of crises or disasters as it is set in a re-imagination of the Black Death plague roughly within the south-western region of fourteenth century France, but one that personifies the real Black Death with a plague of supernatural flesh-eating rats and places players into the role of two fictional orphan children. This presentation seeks to demonstrate historical games’ value for teaching and learning by discussing A Plague Tale: Innocence’s fantastical adaptation of the plague as a model for exploring the role of re-mediation, and subversion, of past pre-modern folklore imaginations and beliefs. Fantasy or folkloric treatment of historical events or periods can provide insight into the experiential dimensions of a period in history re-told as an interactive folktale.

year: 2021

title: The DNA Binding Protein Lsr2 from Mycobacterium tuberculosis

authors: Summers, Emma Louise

subjects: DNA binding protein

abstract: Lsr2 is a small, basic DNA binding protein that is highly conserved in mycobacteria and related actinomycetes. Lsr2 is essential for growth in Mycobacterium tuberculosis and previous studies have shown that Lsr2 is involved in down-regulating a wide range of genes involved in cell wall synthesis and metabolic functions. This regulatory function is likely to influence bacterial growth and survival.

This research investigated the biochemistry and 3D structure of Lsr2 from M. tuberculosis. Transmission electron microscopy (TEM) analysis of Lsr2 in complex with DNA revealed a regular fibril-like arrangement of protein coating double-stranded DNA. In addition, it was shown that Lsr2 physically protected DNA from DNase activity. The structure of the C-terminal DNA binding domain of Lsr2, determined by others part way through this research, prompted site directed mutagenesis of residues proposed to interact with DNA. Modification of arginine residues significantly reduced the binding of Lsr2 to DNA and fibril-like structures were not observed using TEM, for arginine mutants.

The first crystal structure of the N-terminal domain of Lsr2 is reported here. Two high resolution structures in monoclinic and hexagonal space groups were solved using X-ray crystallography and ab initio phasing. Proteolytic processing of the N-terminus of Lsr2 was revealed by the structure in P2₁ and this process was recreated using the protease trypsin which resulted in crystal formation in a P3₁21 space group. Both structures show linear chains of dimeric N-terminal Lsr2, as shown by crystallographic symmetry, linked by overlapping anti-parallel β-sheets, revealing a mechanism of protein oligomerisation. Oligomerisation only occurs after the removal of the first three residues M1, A2 and K3. In solution, protein oligomerisation was recreated with trypsin, resulting in the formation of large protein complexes. A change in DNA topology after the addition of trypsin to full-length Lsr2/DNA complexes was observed using TEM. This mechanism is likely to be important to M. tuberculosis under “stress” conditions where proteases are known to be upregulated and where cross-linking and condensation of DNA is critical.

text: The DNA Binding Protein Lsr2 from Mycobacterium tuberculosis Lsr2 is a small, basic DNA binding protein that is highly conserved in mycobacteria and related actinomycetes. Lsr2 is essential for growth in Mycobacterium tuberculosis and previous studies have shown that Lsr2 is involved in down-regulating a wide range of genes involved in cell wall synthesis and metabolic functions. This regulatory function is likely to influence bacterial growth and survival.

This research investigated the biochemistry and 3D structure of Lsr2 from M. tuberculosis. Transmission electron microscopy (TEM) analysis of Lsr2 in complex with DNA revealed a regular fibril-like arrangement of protein coating double-stranded DNA. In addition, it was shown that Lsr2 physically protected DNA from DNase activity. The structure of the C-terminal DNA binding domain of Lsr2, determined by others part way through this research, prompted site directed mutagenesis of residues proposed to interact with DNA. Modification of arginine residues significantly reduced the binding of Lsr2 to DNA and fibril-like structures were not observed using TEM, for arginine mutants.

The first crystal structure of the N-terminal domain of Lsr2 is reported here. Two high resolution structures in monoclinic and hexagonal space groups were solved using X-ray crystallography and ab initio phasing. Proteolytic processing of the N-terminus of Lsr2 was revealed by the structure in P2₁ and this process was recreated using the protease trypsin which resulted in crystal formation in a P3₁21 space group. Both structures show linear chains of dimeric N-terminal Lsr2, as shown by crystallographic symmetry, linked by overlapping anti-parallel β-sheets, revealing a mechanism of protein oligomerisation. Oligomerisation only occurs after the removal of the first three residues M1, A2 and K3. In solution, protein oligomerisation was recreated with trypsin, resulting in the formation of large protein complexes. A change in DNA topology after the addition of trypsin to full-length Lsr2/DNA complexes was observed using TEM. This mechanism is likely to be important to M. tuberculosis under “stress” conditions where proteases are known to be upregulated and where cross-linking and condensation of DNA is critical.

year: 2012

title: Riperata kahutia: a woman of mana

authors: Nikora, Shelley Aroha

subjects: Riperata Kahutia

abstract: Riperata Kahutia is widely remembered as a leader in the history of Tūranganuiā-
Kiwa, who fought to retain and repossess land during the difficult times of the
1860's. During this period of rapid social change and political upheavals resulting
in injustices, the exercise of rangatiratanga took many forms. Responses to
change covered a wide spectrum and there was no 'right' way of doing things,
simply leaders making decisions on the basis of what they and their people
thought at the time were best. Riperata utilised the law as a means of retaining
the land and the mana of her people. While her status as a rangatira was never in
doubt, innuendo surfaced suggesting she may have received more land entitlement
than she was qualified for.
The activities by Riperata may have been different from other leaders but the
purpose was the same: to preserve the people and the land. Her strategies for
doing so, based on her inherited mana and upbringing, her knowledge of local iwi
traditions, her acquisition of new skills introduced by Pākehā and her innovative
approaches to exercising her rangatiratanga, make her a worthwhile study.
This thesis will support the viewpoint that Riperata Kahutia acted on behalf of her
people and for the good of her people. It will be argued Riperata was a visionary
who embraced the changes imposed upon a society enduring the consequences of
colonisation. A major objective is to dispel suggestions she embellished her
rights.

text: Riperata kahutia: a woman of mana Riperata Kahutia is widely remembered as a leader in the history of Tūranganuiā-
Kiwa, who fought to retain and repossess land during the difficult times of the
1860's. During this period of rapid social change and political upheavals resulting
in injustices, the exercise of rangatiratanga took many forms. Responses to
change covered a wide spectrum and there was no 'right' way of doing things,
simply leaders making decisions on the basis of what they and their people
thought at the time were best. Riperata utilised the law as a means of retaining
the land and the mana of her people. While her status as a rangatira was never in
doubt, innuendo surfaced suggesting she may have received more land entitlement
than she was qualified for.
The activities by Riperata may have been different from other leaders but the
purpose was the same: to preserve the people and the land. Her strategies for
doing so, based on her inherited mana and upbringing, her knowledge of local iwi
traditions, her acquisition of new skills introduced by Pākehā and her innovative
approaches to exercising her rangatiratanga, make her a worthwhile study.
This thesis will support the viewpoint that Riperata Kahutia acted on behalf of her
people and for the good of her people. It will be argued Riperata was a visionary
who embraced the changes imposed upon a society enduring the consequences of
colonisation. A major objective is to dispel suggestions she embellished her
rights.

year: 2009