title: A Comparative Analysis of Equine Mesenchymal Stromal Cells and Dermal Fibroblasts

authors: Leander, Alexandra Anneli Kristina

abstract: The aim of this study was to isolate and characterise equine mesenchymal stromal cells from various sources and to compare their biological properties to that of dermal fibroblasts. By using multiple conventional methods a comparative analysis of the different populations could be made. The results showed that little distinguishes mesenchymal stromal cells from dermal fibroblasts, they have the same morphology and growth characteristics, the same cell surface markers as demonstrated with immunocytochemistry and flow cytometry and the capacity to undergo trilineage differentiation into adipo-, chondro- and osteogenic lineages essentially becoming fat, cartilage and bone. An allogenic synovial fluid model was shown to induce a chondrogenic phenotype in equine mesenchymal stromal cells as well as dermal fibroblasts. A bio-activation assay was performed to evaluate cytokine production of cell populations after activation with Tumour necrosis factor alpha or inflammatory synovial fluid. All of the analysed cell populations demonstrated up-regulation of production of the anti-inflammatory cytokines IL-10 and TGF-β1, after activation with inflammatory synovial fluid, although significant values could not be obtained for every source. As for Prostaglandin E2, results were obtained for bone marrow stromal cells and dermal fibroblasts populations only. Their effects were in opposite; dermal fibroblast reduced the production of Prostaglandin E2 post activation. A functional bio-activation assay has shown promise as a method to distinguish equine mesenchymal stromal cells from dermal fibroblast populations.

text: A Comparative Analysis of Equine Mesenchymal Stromal Cells and Dermal Fibroblasts The aim of this study was to isolate and characterise equine mesenchymal stromal cells from various sources and to compare their biological properties to that of dermal fibroblasts. By using multiple conventional methods a comparative analysis of the different populations could be made. The results showed that little distinguishes mesenchymal stromal cells from dermal fibroblasts, they have the same morphology and growth characteristics, the same cell surface markers as demonstrated with immunocytochemistry and flow cytometry and the capacity to undergo trilineage differentiation into adipo-, chondro- and osteogenic lineages essentially becoming fat, cartilage and bone. An allogenic synovial fluid model was shown to induce a chondrogenic phenotype in equine mesenchymal stromal cells as well as dermal fibroblasts. A bio-activation assay was performed to evaluate cytokine production of cell populations after activation with Tumour necrosis factor alpha or inflammatory synovial fluid. All of the analysed cell populations demonstrated up-regulation of production of the anti-inflammatory cytokines IL-10 and TGF-β1, after activation with inflammatory synovial fluid, although significant values could not be obtained for every source. As for Prostaglandin E2, results were obtained for bone marrow stromal cells and dermal fibroblasts populations only. Their effects were in opposite; dermal fibroblast reduced the production of Prostaglandin E2 post activation. A functional bio-activation assay has shown promise as a method to distinguish equine mesenchymal stromal cells from dermal fibroblast populations.

year: 2017

title: Solvent-free mechanochemical synthesis of π–π stacked arene–perfluoroarene co-crystals

authors: Saunders, Graham C.

subjects: mechanochemical synthesis

abstract: The 1:1 π–π stacked co-crystals of naphthalene–octafluoronaphthalene and biphenyl–decafluorobiphenyl were synthesized by manual grinding of arene and perfluoroarene.

text: Solvent-free mechanochemical synthesis of π–π stacked arene–perfluoroarene co-crystals The 1:1 π–π stacked co-crystals of naphthalene–octafluoronaphthalene and biphenyl–decafluorobiphenyl were synthesized by manual grinding of arene and perfluoroarene.

year: 2013

title: Weaving kaupapa Māori and e-Learning

authors: Neal, Terry

subjects: New Zealand

abstract: This paper describes the creation and design of an online professional development course that aims to help educators in New Zealand to weave kaupapa Māori and e-Learning approaches together. The team sought to model weaving of kaupapa Māori and e-Learning approaches in how they worked together and the educational design for the material. The authors propose that educators with these skills are crucial if we are to effectively meet the needs of Māori learners, more than that, of all New Zealand learners, in the 21st century.

text: Weaving kaupapa Māori and e-Learning This paper describes the creation and design of an online professional development course that aims to help educators in New Zealand to weave kaupapa Māori and e-Learning approaches together. The team sought to model weaving of kaupapa Māori and e-Learning approaches in how they worked together and the educational design for the material. The authors propose that educators with these skills are crucial if we are to effectively meet the needs of Māori learners, more than that, of all New Zealand learners, in the 21st century.

year: 2006-09