title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

authors: Jacobsen, Carsten Suhr

subjects: Science & Technology

abstract: Here we show that a commercial blocking reagent (G2) based on modifed eukaryotic DNA signifcantly improved DNA extraction efciency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the number of PCR-amplifed16S rRNA genes recovered from biomass naturally present in the soils by one log unit. An extensive sequencing approach demonstrated that the blocking reagent was free of contaminating DNA, and may therefore also be used in metagenomics studies that require direct sequencing.

text: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples Here we show that a commercial blocking reagent (G2) based on modifed eukaryotic DNA signifcantly improved DNA extraction efciency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the number of PCR-amplifed16S rRNA genes recovered from biomass naturally present in the soils by one log unit. An extensive sequencing approach demonstrated that the blocking reagent was free of contaminating DNA, and may therefore also be used in metagenomics studies that require direct sequencing.

year: 2018-04-09

title: A study of zinc complexes of the metalloligand [Pt₂(μ-S)₂(PPh₃)₄] of the type [Pt₂(μ-S)₂(PPh₃)₄ZnL]⁺ (L = bidentate chelating ligand)

authors: Henderson, William

subjects: platinum complexes

abstract: Reactions of [Pt₂(μ-S)₂(PPh₃)₄] with zinc acetate and an ancillary chelating ligand L (HL = 8-hydroxyquinoline, 8-tosylaminoquinoline or maltol) with added trimethylamine in methanol give new cationic platinum–zinc sulfide aggregates [Pt₂(μ-S)₂(PPh₃)₄ZnL]+, isolated as their BF4− salts. The complexes were characterized by NMR spectroscopy, ESI mass spectrometry, microelemental analysis, and an X-ray structure determination of the tosylamidoquinoline derivative [Pt₂(μ-S)₂(PPh₃)₄Zn(TAQ)]BF₄, which showed a distorted tetrahedral coordination geometry at zinc. Additional examples, containing picolinate, dithiocarbamate, or dithiophosphinate ligands were also synthesized and partly characterized in order to demonstrate a wider range of available derivatives.

text: A study of zinc complexes of the metalloligand [Pt₂(μ-S)₂(PPh₃)₄] of the type [Pt₂(μ-S)₂(PPh₃)₄ZnL]⁺ (L = bidentate chelating ligand) Reactions of [Pt₂(μ-S)₂(PPh₃)₄] with zinc acetate and an ancillary chelating ligand L (HL = 8-hydroxyquinoline, 8-tosylaminoquinoline or maltol) with added trimethylamine in methanol give new cationic platinum–zinc sulfide aggregates [Pt₂(μ-S)₂(PPh₃)₄ZnL]+, isolated as their BF4− salts. The complexes were characterized by NMR spectroscopy, ESI mass spectrometry, microelemental analysis, and an X-ray structure determination of the tosylamidoquinoline derivative [Pt₂(μ-S)₂(PPh₃)₄Zn(TAQ)]BF₄, which showed a distorted tetrahedral coordination geometry at zinc. Additional examples, containing picolinate, dithiocarbamate, or dithiophosphinate ligands were also synthesized and partly characterized in order to demonstrate a wider range of available derivatives.

year: 2013

title: Te kū o te kupu

authors: Raerino, Heddell Robert

subjects: Māori language

abstract: Ko tēnei tuhingaroa, he rangahau hai wherawhera ake i te āhuatanga o te reo Māori, e kiia ana ko tōna oro, me te kū o ngā kupu kōrero o taua reo. Ko te aronga whakaaro o tēnei rangahau, he rapu i tētahi āhuatanga kāore anō kia tirotirohia, kia tuhia rānei mō te reo Māori. Ehara tēnei rangahau i te kaupapa, i te mahi hou. Otirā, kai te whai te kaituhi i ngā rangahau kua oti kē i wētahi tohunga te wānanga mō wētahi atu reo. Ko tā te kaituhi he whai i ngā tauira a te hunga wetewete reo nei, ka uwhi ai ki runga i te reo Māori hai āwhina i ngā tātaritanga ā te kaituhi mō tōna ake reo, arā, te reo Māori.

Kai te upoko tuatahi ka whaia ngā rerenga whakaaro i pūpū ake ai te taitara 'te kū o te kupu' mō tēnei mahi rangahau. Inā ka tirohia i ahu ngā oro o te reo mai i whea e ai ki tā te Māori wānanga. Mai i ngā oro ka puta ko ngā ku o ngā kupu me ngā kupu o ngā rārangi kōrero.

Hai te upoko tuarua ka tirohia ngā kōrero kua tuhia e ngā tohunga wetewete reo e pā ana ki te kaupapa o tēnei tuhingaroa, arā, te kū o te kupu. Ko ngā tirohanga mō te reo ake, arā, i ahu mai i whea, he aha ōna momo whakaata me tōna pakaritanga ka tirohia i roto i te upoko tuatoru.

Mai i ngā momo whakaata o te reo i roto i ngā momo waiata, ka āta tahuri te kaituhi ki te whakawhānui haere i tana wetewete me tāna tūhononga i ngā rerenga me ngā pekanga o te ku o te kupu.

Kai te upoko tuarima ka tirohia ngā whāinga me ngā aronga ā wētahi kaitito
waiata Māori me wētahi kaikōrero i te reo Māori, mō ō rātau māramatanga ki ngā kū me ngā oro o te reo Māori.

Kai te upoko tuaono e whāriki ana wētahi tauira kōrero Māori, hai whakaata i ngā momo orōmāhara, oro ohorere, ororite, kupu whakahuarua me wērā atu tauira whakaahua i ngā kū o te kupu me ngā oro o te reo Māori.

Ko ngā hua o tēnei rangahau, o tēnei tuhingaroa ka kitea i roto i te upoko
tuawhitu. Kōinei te whakakopinga o tēnei mahi.

text: Te kū o te kupu Ko tēnei tuhingaroa, he rangahau hai wherawhera ake i te āhuatanga o te reo Māori, e kiia ana ko tōna oro, me te kū o ngā kupu kōrero o taua reo. Ko te aronga whakaaro o tēnei rangahau, he rapu i tētahi āhuatanga kāore anō kia tirotirohia, kia tuhia rānei mō te reo Māori. Ehara tēnei rangahau i te kaupapa, i te mahi hou. Otirā, kai te whai te kaituhi i ngā rangahau kua oti kē i wētahi tohunga te wānanga mō wētahi atu reo. Ko tā te kaituhi he whai i ngā tauira a te hunga wetewete reo nei, ka uwhi ai ki runga i te reo Māori hai āwhina i ngā tātaritanga ā te kaituhi mō tōna ake reo, arā, te reo Māori.

Kai te upoko tuatahi ka whaia ngā rerenga whakaaro i pūpū ake ai te taitara 'te kū o te kupu' mō tēnei mahi rangahau. Inā ka tirohia i ahu ngā oro o te reo mai i whea e ai ki tā te Māori wānanga. Mai i ngā oro ka puta ko ngā ku o ngā kupu me ngā kupu o ngā rārangi kōrero.

Hai te upoko tuarua ka tirohia ngā kōrero kua tuhia e ngā tohunga wetewete reo e pā ana ki te kaupapa o tēnei tuhingaroa, arā, te kū o te kupu. Ko ngā tirohanga mō te reo ake, arā, i ahu mai i whea, he aha ōna momo whakaata me tōna pakaritanga ka tirohia i roto i te upoko tuatoru.

Mai i ngā momo whakaata o te reo i roto i ngā momo waiata, ka āta tahuri te kaituhi ki te whakawhānui haere i tana wetewete me tāna tūhononga i ngā rerenga me ngā pekanga o te ku o te kupu.

Kai te upoko tuarima ka tirohia ngā whāinga me ngā aronga ā wētahi kaitito
waiata Māori me wētahi kaikōrero i te reo Māori, mō ō rātau māramatanga ki ngā kū me ngā oro o te reo Māori.

Kai te upoko tuaono e whāriki ana wētahi tauira kōrero Māori, hai whakaata i ngā momo orōmāhara, oro ohorere, ororite, kupu whakahuarua me wērā atu tauira whakaahua i ngā kū o te kupu me ngā oro o te reo Māori.

Ko ngā hua o tēnei rangahau, o tēnei tuhingaroa ka kitea i roto i te upoko
tuawhitu. Kōinei te whakakopinga o tēnei mahi.

year: 2000