title: The chronology of Waikato pā: A spatio-temporal investigation of pā in the middle Waikato basin

authors: McBride, Rowan

subjects: pā

abstract: This study considers the chronology of pā construction, proliferation, and development within the 
Middle Waikato Basin through a spatio-temporal investigation of palisade construction activity as 
identified at six pā (fortified places). The chronological approach applied in this thesis uses 14C 
wiggle-match dating to provide an accurate and precise calendar date for the felling of a tree 
(Felling Date) used to construct a palisade defence at a pā. These Felling Dates are interpreted using 
the theory of time perspectivism, focusing on three distinct scales of analysis: Event, Local, and 
Regional. The event scale of analysis successfully produced twenty high-precision felling dates on 
posts recovered from Lake Mangakaware 1 (MA1), Lake Mangakaware 2 (MA2), Lake Mangahia 
(MGA), Taraheke Pā (TAR), Te Uapata Pā (TEU) and Lake Rotokauri (ROT). The local scale 
analysis utilised these felling dates to track the development of the identified palisade defences 
over time, identifying multiple palisade construction phases, representing construction, repair and 
redevelopment episodes at MA1, MA2, MGA, TAR and TEU. Finally, the regional scale analysis 
integrated this evidence into a broader chronological framework, identifying shared episodes of 
palisade construction activity within the Middle Waikato Basin. This analysis highlights three 
critical periods of proposed socio-political stress and stability between the late fifteenth and early 
nineteenth centuries, related to hypothesised influential variables from the broader cultural and 
archaeological landscape that are proposed to be responsible for the emergence, proliferation and 
development of pā over time.

text: The chronology of Waikato pā: A spatio-temporal investigation of pā in the middle Waikato basin This study considers the chronology of pā construction, proliferation, and development within the 
Middle Waikato Basin through a spatio-temporal investigation of palisade construction activity as 
identified at six pā (fortified places). The chronological approach applied in this thesis uses 14C 
wiggle-match dating to provide an accurate and precise calendar date for the felling of a tree 
(Felling Date) used to construct a palisade defence at a pā. These Felling Dates are interpreted using 
the theory of time perspectivism, focusing on three distinct scales of analysis: Event, Local, and 
Regional. The event scale of analysis successfully produced twenty high-precision felling dates on 
posts recovered from Lake Mangakaware 1 (MA1), Lake Mangakaware 2 (MA2), Lake Mangahia 
(MGA), Taraheke Pā (TAR), Te Uapata Pā (TEU) and Lake Rotokauri (ROT). The local scale 
analysis utilised these felling dates to track the development of the identified palisade defences 
over time, identifying multiple palisade construction phases, representing construction, repair and 
redevelopment episodes at MA1, MA2, MGA, TAR and TEU. Finally, the regional scale analysis 
integrated this evidence into a broader chronological framework, identifying shared episodes of 
palisade construction activity within the Middle Waikato Basin. This analysis highlights three 
critical periods of proposed socio-political stress and stability between the late fifteenth and early 
nineteenth centuries, related to hypothesised influential variables from the broader cultural and 
archaeological landscape that are proposed to be responsible for the emergence, proliferation and 
development of pā over time.

year: 2024

title: An Evaluation of an Impaired Driver Treatment Programme Facilitated by Tūhoe Hauora

authors: Waru, Nicole Arihia

subjects: Tuhoe Hauora

abstract: Driving while impaired is a substantial issue in Aotearoa.  It has the potential to result in loss of life and cause significant health and economic strain on society.  Recidivist impaired drivers are major contributors to this problem.  Countermeasures of impaired driving have previously included liquor licencing, drinking age restrictions, health promotion campaigns and health rehabilitation therapies.  The Ministry of Health offer an Impaired Driving therapy throughout Aotearoa as countermeasure to this issue.  The programme is administered through the funder-contract system. Participation is compulsory for those mandated by court order.  
This study evaluates an Impaired Driver programme facilitated by Tūhoe Hauora; a kaupapa Māori mental health and addiction provider based in Taneatua, Eastern Bay of Plenty.  The course is delivered once weekly over a period of ten weeks to a group of twelve or less recidivist impaired drivers or first time offenders who were convicted of driving with excessive levels of breath alcohol.    
The evaluation draws from a community psychology paradigm in exploration of the lived experiences of programme participants, in the context of course 2 of the 2014 Tūhoe Hauora Impaired Driving course.  
The experiences of five individuals were explored using in-depth face to face interviews and presented using a case-by-case approach.  Participant observation, document review, evaluation visits and participant surveys were used as supplement methods of inquiry.  The findings illustrated that Tūhoe Hauora met contract requirements by addressing impaired driving recidivism to date of interview.  The evaluation also found the course actualising the mission of Tūhoe Hauora which was to address the holistic well-being of programme participants.  The research did present areas in the programme that could be improved particularly pertaining to the referral process and a need for agency collaboration.  Recommendations were formulated in attempt to address the programme gaps and discussion is offered in addressing the multi-layered oppression that was experienced throughout the evaluation process by both Tūhoe Hauora and the evaluation participants.

text: An Evaluation of an Impaired Driver Treatment Programme Facilitated by Tūhoe Hauora Driving while impaired is a substantial issue in Aotearoa.  It has the potential to result in loss of life and cause significant health and economic strain on society.  Recidivist impaired drivers are major contributors to this problem.  Countermeasures of impaired driving have previously included liquor licencing, drinking age restrictions, health promotion campaigns and health rehabilitation therapies.  The Ministry of Health offer an Impaired Driving therapy throughout Aotearoa as countermeasure to this issue.  The programme is administered through the funder-contract system. Participation is compulsory for those mandated by court order.  
This study evaluates an Impaired Driver programme facilitated by Tūhoe Hauora; a kaupapa Māori mental health and addiction provider based in Taneatua, Eastern Bay of Plenty.  The course is delivered once weekly over a period of ten weeks to a group of twelve or less recidivist impaired drivers or first time offenders who were convicted of driving with excessive levels of breath alcohol.    
The evaluation draws from a community psychology paradigm in exploration of the lived experiences of programme participants, in the context of course 2 of the 2014 Tūhoe Hauora Impaired Driving course.  
The experiences of five individuals were explored using in-depth face to face interviews and presented using a case-by-case approach.  Participant observation, document review, evaluation visits and participant surveys were used as supplement methods of inquiry.  The findings illustrated that Tūhoe Hauora met contract requirements by addressing impaired driving recidivism to date of interview.  The evaluation also found the course actualising the mission of Tūhoe Hauora which was to address the holistic well-being of programme participants.  The research did present areas in the programme that could be improved particularly pertaining to the referral process and a need for agency collaboration.  Recommendations were formulated in attempt to address the programme gaps and discussion is offered in addressing the multi-layered oppression that was experienced throughout the evaluation process by both Tūhoe Hauora and the evaluation participants.

year: 2016

title: The Structure of the Oligomerization Domain of Lsr2 from Mycobacterium tuberculosis Reveals a Mechanism for Chromosome Organization and Protection

authors: Summers, Emma Louise

subjects: biology

abstract: Lsr2 is a small DNA-binding protein present in mycobacteria and related actinobacteria that regulates gene expression and influences the organization of bacterial chromatin. Lsr2 is a dimer that binds to AT-rich regions of chromosomal DNA and physically protects DNA from damage by reactive oxygen intermediates (ROI). A recent structure of the C-terminal DNA-binding domain of Lsr2 provides a rationale for its interaction with the minor groove of DNA, its preference for AT-rich tracts, and its similarity to other bacterial nucleoid-associated DNA-binding domains. In contrast, the details of Lsr2 dimerization (and oligomerization) via its N-terminal domain, and the mechanism of Lsr2-mediated chromosomal cross-linking and protection is unknown. We have solved the structure of the N-terminal domain of Lsr2 (N-Lsr2) at 1.73 Å resolution using crystallographic ab initio approaches. The structure shows an intimate dimer of two ß-ß-a motifs with no close homologues in the structural databases. The organization of individual N-Lsr2 dimers in the crystal also reveals a mechanism for oligomerization. Proteolytic removal of three N-terminal residues from Lsr2 results in the formation of an anti-parallel β-sheet between neighboring molecules and the formation of linear chains of N-Lsr2. Oligomerization can be artificially induced using low concentrations of trypsin and the arrangement of N-Lsr2 into long chains is observed in both monoclinic and hexagonal crystallographic space groups. In solution, oligomerization of N-Lsr2 is also observed following treatment with trypsin. A change in chromosomal topology after the addition of trypsin to full-length Lsr2-DNA complexes and protection of DNA towards DNAse digestion can be observed using electron microscopy and electrophoresis. These results suggest a mechanism for oligomerization of Lsr2 via protease-activation leading to chromosome compaction and protection, and concomitant down-regulation of large numbers of genes. This mechanism is likely to be relevant under conditions of stress where cellular proteases are known to be upregulated.

text: The Structure of the Oligomerization Domain of Lsr2 from Mycobacterium tuberculosis Reveals a Mechanism for Chromosome Organization and Protection Lsr2 is a small DNA-binding protein present in mycobacteria and related actinobacteria that regulates gene expression and influences the organization of bacterial chromatin. Lsr2 is a dimer that binds to AT-rich regions of chromosomal DNA and physically protects DNA from damage by reactive oxygen intermediates (ROI). A recent structure of the C-terminal DNA-binding domain of Lsr2 provides a rationale for its interaction with the minor groove of DNA, its preference for AT-rich tracts, and its similarity to other bacterial nucleoid-associated DNA-binding domains. In contrast, the details of Lsr2 dimerization (and oligomerization) via its N-terminal domain, and the mechanism of Lsr2-mediated chromosomal cross-linking and protection is unknown. We have solved the structure of the N-terminal domain of Lsr2 (N-Lsr2) at 1.73 Å resolution using crystallographic ab initio approaches. The structure shows an intimate dimer of two ß-ß-a motifs with no close homologues in the structural databases. The organization of individual N-Lsr2 dimers in the crystal also reveals a mechanism for oligomerization. Proteolytic removal of three N-terminal residues from Lsr2 results in the formation of an anti-parallel β-sheet between neighboring molecules and the formation of linear chains of N-Lsr2. Oligomerization can be artificially induced using low concentrations of trypsin and the arrangement of N-Lsr2 into long chains is observed in both monoclinic and hexagonal crystallographic space groups. In solution, oligomerization of N-Lsr2 is also observed following treatment with trypsin. A change in chromosomal topology after the addition of trypsin to full-length Lsr2-DNA complexes and protection of DNA towards DNAse digestion can be observed using electron microscopy and electrophoresis. These results suggest a mechanism for oligomerization of Lsr2 via protease-activation leading to chromosome compaction and protection, and concomitant down-regulation of large numbers of genes. This mechanism is likely to be relevant under conditions of stress where cellular proteases are known to be upregulated.

year: 2012