title: Watching news : a study of television news reception

authors: Bosomworth, John Forbes

abstract: This doctoral thesis is an investigation of the relationship that exists between viewers and television news. As an inherently reflexive thesis, the research process itself is also critically examined. This duality is reflected in the basic structure of the thesis, with Part One - Praxis - focusing on various aspects of the research process itself, and Part Two - Analysis - dealing with the nature of the viewer-news relationship. Adopting a discourse-oriented approach, the analysis is based on semi-structured interviews with eleven individual viewers. Originally conceived as an exercise in elicitation, the introduction of feminist research perspective’s in conjunction with a growing sense of reflexivity led to a transformation whereby the interviews were reconfigured instead as sites of meaning construction. As discourses of reception, the interviews showed the news-viewer relationship to be characterised by a dynamic tension between being pulled into the encounter and pushed away from it. A bi-dimensional framework of analysis is employed, with one dimension focusing on the relational aspect of the viewer-news relationship and the other on the interpretive aspect. Using this framework, the discourses of reception are then contextually (i.e. as specific case studies in reception) and thematically analysed. The thesis concludes by suggesting that reception, as a specific example of cultural consumption, can be seen as a form of guerrilla warfare where the strategies of news producers are confronted by the tactics of viewers.

text: Watching news : a study of television news reception This doctoral thesis is an investigation of the relationship that exists between viewers and television news. As an inherently reflexive thesis, the research process itself is also critically examined. This duality is reflected in the basic structure of the thesis, with Part One - Praxis - focusing on various aspects of the research process itself, and Part Two - Analysis - dealing with the nature of the viewer-news relationship. Adopting a discourse-oriented approach, the analysis is based on semi-structured interviews with eleven individual viewers. Originally conceived as an exercise in elicitation, the introduction of feminist research perspective’s in conjunction with a growing sense of reflexivity led to a transformation whereby the interviews were reconfigured instead as sites of meaning construction. As discourses of reception, the interviews showed the news-viewer relationship to be characterised by a dynamic tension between being pulled into the encounter and pushed away from it. A bi-dimensional framework of analysis is employed, with one dimension focusing on the relational aspect of the viewer-news relationship and the other on the interpretive aspect. Using this framework, the discourses of reception are then contextually (i.e. as specific case studies in reception) and thematically analysed. The thesis concludes by suggesting that reception, as a specific example of cultural consumption, can be seen as a form of guerrilla warfare where the strategies of news producers are confronted by the tactics of viewers.

year: 2000

title: Role of growth factors in the regulation of skeletal muscle mass, fibre type and fibre size

authors: Martyn, Julie Anne Kerr

abstract: Growth factors play an important role in the regulation of the growth and development of many tissues including muscle. Muscle is derived from somitic cells that differentiate to form myoblasts. These myoblasts then fuse to form myofibres. As these myofibres mature they take on the morphological, biochemical and physiological properties of mature muscle fibres. It is the plasticity of muscle fibres with respect to these properties that gives skeletal muscle its remarkable ability to adapt to different conditions of loading and usage. The aim of this thesis was to determine the role of growth factors in regulating muscle fibre type and fibre size in two different models of altered muscle mass.

Bovine muscle development was examined in normal foetuses (NM) and in double-muscled Belgian Blue foetuses (DM) carrying a mutation in the gene for myostatin. Muscle fibre type and fibre size were characterised using semi-automated computer-assisted image analysis after myofibrillar ATPase histochemical staining. Plasma IGF levels were measured, IGF-1 mRNA was measured using in situ hybridisation and IGF receptor binding was analysed by receptor autoradiography. Myostatin mRNA was analysed using semi-quantitative RT-PCR.

Skeletal muscle hypertrophy and atrophy were induced m adult rabbits by immobilisation of the hind limb with muscles in a lengthened and shortened position respectively. Fibre type and fibre size changes were analysed as described above and myostatin mRNA expression levels were measured using Northern blot analysis and semi-quantitative RT-PCR.

Results showed there was a biphasic change in the percentage of type 1 muscle fibres in both NM and DM, with the percentage initially declining, then increasing again. The percentage of type 1 fibres was consistently lower in DM. Circulating levels of IGF-1 increased with gestational age and levels of IGF-1 and IGF-2 were not different between NM and DM. IGF-1 mRNA expression in skeletal muscle declined with age in a pattern that was unrelated to changes in fibre type. Levels of IGF-1 mRNA were lower in DM during late gestation and this decrease was temporally linked to a reduced area of type 1 fibres in DM at this stage of development. Myostatin mRNA declined with gestational age, and levels of the mutant mRNA were higher in DM.

In induced hypertrophy, myostatin mRNA expression was lower than in controls and in atrophy, myostatin mRNA was elevated at 2 days but not at 6 days. There were no changes in fibre type or fibre size over the treatment period. Myostatin protein was evenly localised to all muscle fibre types.

In conclusion, IGF-1 appears to be unrelated to changes in fibre type during bovine muscle development, but may play a role in the regulation of fibre size. The DM condition is associated with a change in fibre type, but the role of myostatin in mediating this change remains unknown. Changes in myostatin mRNA in response to induced hypertrophy and atrophy strongly suggest an important role for myostatin as a negative regulator of skeletal muscle mass in postnatal animals.

text: Role of growth factors in the regulation of skeletal muscle mass, fibre type and fibre size Growth factors play an important role in the regulation of the growth and development of many tissues including muscle. Muscle is derived from somitic cells that differentiate to form myoblasts. These myoblasts then fuse to form myofibres. As these myofibres mature they take on the morphological, biochemical and physiological properties of mature muscle fibres. It is the plasticity of muscle fibres with respect to these properties that gives skeletal muscle its remarkable ability to adapt to different conditions of loading and usage. The aim of this thesis was to determine the role of growth factors in regulating muscle fibre type and fibre size in two different models of altered muscle mass.

Bovine muscle development was examined in normal foetuses (NM) and in double-muscled Belgian Blue foetuses (DM) carrying a mutation in the gene for myostatin. Muscle fibre type and fibre size were characterised using semi-automated computer-assisted image analysis after myofibrillar ATPase histochemical staining. Plasma IGF levels were measured, IGF-1 mRNA was measured using in situ hybridisation and IGF receptor binding was analysed by receptor autoradiography. Myostatin mRNA was analysed using semi-quantitative RT-PCR.

Skeletal muscle hypertrophy and atrophy were induced m adult rabbits by immobilisation of the hind limb with muscles in a lengthened and shortened position respectively. Fibre type and fibre size changes were analysed as described above and myostatin mRNA expression levels were measured using Northern blot analysis and semi-quantitative RT-PCR.

Results showed there was a biphasic change in the percentage of type 1 muscle fibres in both NM and DM, with the percentage initially declining, then increasing again. The percentage of type 1 fibres was consistently lower in DM. Circulating levels of IGF-1 increased with gestational age and levels of IGF-1 and IGF-2 were not different between NM and DM. IGF-1 mRNA expression in skeletal muscle declined with age in a pattern that was unrelated to changes in fibre type. Levels of IGF-1 mRNA were lower in DM during late gestation and this decrease was temporally linked to a reduced area of type 1 fibres in DM at this stage of development. Myostatin mRNA declined with gestational age, and levels of the mutant mRNA were higher in DM.

In induced hypertrophy, myostatin mRNA expression was lower than in controls and in atrophy, myostatin mRNA was elevated at 2 days but not at 6 days. There were no changes in fibre type or fibre size over the treatment period. Myostatin protein was evenly localised to all muscle fibre types.

In conclusion, IGF-1 appears to be unrelated to changes in fibre type during bovine muscle development, but may play a role in the regulation of fibre size. The DM condition is associated with a change in fibre type, but the role of myostatin in mediating this change remains unknown. Changes in myostatin mRNA in response to induced hypertrophy and atrophy strongly suggest an important role for myostatin as a negative regulator of skeletal muscle mass in postnatal animals.

year: 1999

title: An investigation into the commercial feasibility of Jasus edwardsii aquaculture in New Zealand

authors: Ruru, Ian Hector

subjects: New Zealand

abstract: In 1996 New Zealand introduced legislation to allow Jasus edwardsii pueruli to be collected as "seedstock" and ongrown in a commercial aquaculture trial. The aim of this thesis was to investigate the commercial feasibility of J. edwardsii aquaculture in New Zealand by working with an organisation involved in the trial.
The methodology involved a combination of experiments in the field, in laboratories, and within an ongrowing facility. This thesis examined five key areas and found the following:
1. Data obtained in the research indicates that New Zealand will continue to rely on harvesting pueruli from the wild since significant technical obstacles remain in the development of commercial scale pueruli hatcheries. Forecast data, obtained using the delphi technique and information about current research on larval rearing, indicates that commercial scale supply of pueruli from hatcheries may occur between 2017 and 2021.
2. Harvesting trials collected a low number of pueruli with the lowest unit cost for collection being $1.80. A greater collection rate could be achieved with a better understanding of seasonal and local settlement patterns.
3. Survival rates in pueruli transporting experiments were significantly affected by stocking density, time and temperature. The safest transit conditions were achieved with 15 pueruli per litre for less than 6 hours at 14°C.
4. A range of stocking densities, from 3 lobsters per tank to 12 per tank, were tested for their effect on growth of six-month old lobsters. The lobsters were reared in a total recirculation seawater system at a constant 16°C, and fed fresh mussels (Perna canaliculus). For the 121 day trial, no statistically significant effect was detected. However, there was a trend for growth rates to be highest at 3 and 6 lobsters per tank and for growth rates at stocking densities of 9 and 12 lobsters per tank to decrease after Day 50. Lobsters showed increased mortality at the highest stocking rate.
5. A bioeconomic model of a hypothetical farm was developed to assess economic benefits and risks and to determine overall profitability. The model showed an annual net cash position of $287,100 and cumulative cash position of $859,200 by the 10th year of operation based on annual sales of 10,837 kg at $65 per kg. The model indicates that the highest cost components in the first 10 years are processing and transport, and labour. Based on the model and the scenarios examined, profitability is very sensitive to factors such as the farm size, processing and transport costs, and sale price. Biological factors such as growth rates, survival, and feed requirements also influence overall profitability. Good returns will depend upon consistent exports of high quality lobsters.
The study concludes that commercial feasibility will be affected by these key areas. The potential of J. edwardsii aquaculture in New Zealand will continue to benefit from further research.

text: An investigation into the commercial feasibility of Jasus edwardsii aquaculture in New Zealand In 1996 New Zealand introduced legislation to allow Jasus edwardsii pueruli to be collected as "seedstock" and ongrown in a commercial aquaculture trial. The aim of this thesis was to investigate the commercial feasibility of J. edwardsii aquaculture in New Zealand by working with an organisation involved in the trial.
The methodology involved a combination of experiments in the field, in laboratories, and within an ongrowing facility. This thesis examined five key areas and found the following:
1. Data obtained in the research indicates that New Zealand will continue to rely on harvesting pueruli from the wild since significant technical obstacles remain in the development of commercial scale pueruli hatcheries. Forecast data, obtained using the delphi technique and information about current research on larval rearing, indicates that commercial scale supply of pueruli from hatcheries may occur between 2017 and 2021.
2. Harvesting trials collected a low number of pueruli with the lowest unit cost for collection being $1.80. A greater collection rate could be achieved with a better understanding of seasonal and local settlement patterns.
3. Survival rates in pueruli transporting experiments were significantly affected by stocking density, time and temperature. The safest transit conditions were achieved with 15 pueruli per litre for less than 6 hours at 14°C.
4. A range of stocking densities, from 3 lobsters per tank to 12 per tank, were tested for their effect on growth of six-month old lobsters. The lobsters were reared in a total recirculation seawater system at a constant 16°C, and fed fresh mussels (Perna canaliculus). For the 121 day trial, no statistically significant effect was detected. However, there was a trend for growth rates to be highest at 3 and 6 lobsters per tank and for growth rates at stocking densities of 9 and 12 lobsters per tank to decrease after Day 50. Lobsters showed increased mortality at the highest stocking rate.
5. A bioeconomic model of a hypothetical farm was developed to assess economic benefits and risks and to determine overall profitability. The model showed an annual net cash position of $287,100 and cumulative cash position of $859,200 by the 10th year of operation based on annual sales of 10,837 kg at $65 per kg. The model indicates that the highest cost components in the first 10 years are processing and transport, and labour. Based on the model and the scenarios examined, profitability is very sensitive to factors such as the farm size, processing and transport costs, and sale price. Biological factors such as growth rates, survival, and feed requirements also influence overall profitability. Good returns will depend upon consistent exports of high quality lobsters.
The study concludes that commercial feasibility will be affected by these key areas. The potential of J. edwardsii aquaculture in New Zealand will continue to benefit from further research.

year: 2000