title: Mutations of p53 gene and SV40 sequences in asbestos associated and non- asbestos-associated mesotheliomas

authors: Mayall, Frederick G.

subjects: mesothelioma

abstract: AIM: To examine mesotheliomas for a possible relation between p53 immunostaining, p53 gene mutation, simian virus 40 (SV40), and asbestos exposure. METHODS: Paraffin sections from 11 mesotheliomas were used for p53 immunostaining and also to extract DNA. This was analysed for the presence of mutations in exons 5 to 8 of the p53 gene using a "cold" single strand conformational polymorphism method, together with sequencing. The DNA from the paraffin sections was also used to search for SV40 sequences. A 105 base pair segment at the 3' of the SV40 large T antigen (Tag) was targeted and any PCR amplification products were sequenced to confirm that they were of SV40 origin. EDAX electron microscopic differential mineral fibre counts were performed on dried lung tissue at a specialist referral centre. RESULTS: The fibre counts showed that seven of the mesotheliomas were associated with abnormally high asbestos exposure. Of these, two showed p53 immunostaining, none showed p53 gene mutation, and five showed SV40. Of the four other mesotheliomas, three showed p53 immunostaining, one showed a (silent) p53 mutation, and none showed SV40. The difference in frequency of SV40 detection was significant at the p < 0.05 level. CONCLUSIONS: Immunostaining for the p53 gene was relatively common but p53 mutations were rare in this series. SV40 virus sequence was detected in five of seven asbestos associated mesotheliomas but in none of the non-asbestos-associated mesotheliomas. This suggests there may be a synergistic interaction between asbestos and SV40 in human mesotheliomas. A study with a larger number of cases is needed to investigate these observations further.

text: Mutations of p53 gene and SV40 sequences in asbestos associated and non- asbestos-associated mesotheliomas AIM: To examine mesotheliomas for a possible relation between p53 immunostaining, p53 gene mutation, simian virus 40 (SV40), and asbestos exposure. METHODS: Paraffin sections from 11 mesotheliomas were used for p53 immunostaining and also to extract DNA. This was analysed for the presence of mutations in exons 5 to 8 of the p53 gene using a "cold" single strand conformational polymorphism method, together with sequencing. The DNA from the paraffin sections was also used to search for SV40 sequences. A 105 base pair segment at the 3' of the SV40 large T antigen (Tag) was targeted and any PCR amplification products were sequenced to confirm that they were of SV40 origin. EDAX electron microscopic differential mineral fibre counts were performed on dried lung tissue at a specialist referral centre. RESULTS: The fibre counts showed that seven of the mesotheliomas were associated with abnormally high asbestos exposure. Of these, two showed p53 immunostaining, none showed p53 gene mutation, and five showed SV40. Of the four other mesotheliomas, three showed p53 immunostaining, one showed a (silent) p53 mutation, and none showed SV40. The difference in frequency of SV40 detection was significant at the p < 0.05 level. CONCLUSIONS: Immunostaining for the p53 gene was relatively common but p53 mutations were rare in this series. SV40 virus sequence was detected in five of seven asbestos associated mesotheliomas but in none of the non-asbestos-associated mesotheliomas. This suggests there may be a synergistic interaction between asbestos and SV40 in human mesotheliomas. A study with a larger number of cases is needed to investigate these observations further.

year: 2013

title: Changes in benthic ecosystem properties and functions across sedimentary gradients in estuaries

authors: Pratt, Daniel Robert

subjects: Ecosystem function

abstract: In estuaries, sediment properties dominate the inhabiting flora and fauna and their role in energy flows and nutrient cycling. Whilst sediment transport is a natural, key process, human intervention in estuaries and their catchments has altered the regime of terrigenous sediment loading and pose both short and long-term consequences to ecosystem functioning. Temporary increases in turbidity reduce light availability for primary production by microphytobenthos (MPB) that fuel benthic communities. Long-term alteration of grain size properties changes the distribution of key macrofaunal species and how they interact with their environment, carrying potentially serious implications for the ecological functioning of these systems. Our knowledge of how benthic ecosystems respond to changes in sedimentary regimes is crucial to our ability to project and manage the impacts of environmental change. In this thesis, I investigated the multifaceted effects of increased sediment loading on the benthic biota and their functioning using natural and experimental sedimentary gradients.

An in situ experiment was conducted on an intertidal sandflat to examine the effects of short-term increases in suspended sediment concentration (SSC) on benthic autotrophic (primary production) and heterotrophic processes. In sunlit conditions, increases in SSC led to dramatic declines in net primary production and concomitant increases in NH₄⁺ efflux from the sediment to the water column. Although sediment chlorophyll-⍺ concentration increased with higher levels of SSC, a result that was likely a photoadaptive response to reduced light intensity, SSC reduced O₂ production per unit of chlorophyll -⍺ . SSC had no significant effect on sediment properties or heterotrophic processes such as sediment oxygen consumption or nutrient efflux, suggesting that temporary increases in suspended sediments (within the range of SSC tested) primarily affected photosynthetic processes. 

Sediment properties, macrofaunal diversity and biogeochemical fluxes were measured across natural gradients of silt and clay (hereafter mud) to determine the effects of habitat change associated with chronic sediment loading on the structure and functioning of benthic communities. There were significant declines in measures of macrofaunal diversity and the maximum densities of key bioturbating bivalves (Austrovenus stutchburyi and Macomona liliana) with increased mud content. Concurrently, the maximum rates of sediment oxygen consumption (SOC), NH₄⁺ efflux (a proxy of nutrient regeneration)and biomass standardised gross primary production (GPPChl-⍺) also decreased with increasing mud content. A. stutchburyi contributed disproportionately to variation in SOC and NH₄⁺ efflux, suggesting that losses of strongly interacting key species concomitant with increased sediment mud content could have a significant impact on ecosystem function. The results from this study demonstrate the significant loss of ecosystem function in intertidal sandflats that is likely from increased sediment mud content associated with long-term increases in sedimentation stress. 

The spatial distributions of MPB biomass, macrofaunal grazer abundances and deposit feeding activity were measured across a gradient of sediment mud content to determine relationships between grazers and MPB biomass across transitional sedimentary environments. The density of feeding traces produced by M. Liliana was measured as a proxy of deposit feeding activity by this species. MPB biomass was generally lower in areas with higher deposit feeding activity but this relationship was scale dependent, emerging over larger areas (tens of centimetres) but absent at local (centimetre) scales relative to the animal’s feeding ambit. Despite higher MPB biomass in muddy sediments, feeding trace density was markedly lower, suggesting lower feeding activity and trophic exchange in muddy compared with sandy sediments. The suspension feeding bivalve A. stutchburyi was positively associated with MPB biomass and the interaction between A. stutchburyi density and mud was the strongest predictor of MPB biomass. Thus, non-trophic interactions that potentially facilitate production may override the deleterious effects of grazing on MPB biomass by large macrofaunal species. This thesis demonstrates the high capacity of sandflat systems for primary, secondary production and nutrient regeneration and the degradation of these ecological properties and functions in muddier and more turbid systems. The decline in this functional capacity reflects the alterations of multiple ecological components and their interactions corresponding to habitat change. Defining changes in these interaction networks can improve our ability to track changes in ecosystem functioning and elucidate underlying pathways and potential mechanisms. In particular, this thesis highlights the value of observing changes in these ecological properties and functions across natural and experimental gradients at the appropriate scales in time and space over which stressors operate.

text: Changes in benthic ecosystem properties and functions across sedimentary gradients in estuaries In estuaries, sediment properties dominate the inhabiting flora and fauna and their role in energy flows and nutrient cycling. Whilst sediment transport is a natural, key process, human intervention in estuaries and their catchments has altered the regime of terrigenous sediment loading and pose both short and long-term consequences to ecosystem functioning. Temporary increases in turbidity reduce light availability for primary production by microphytobenthos (MPB) that fuel benthic communities. Long-term alteration of grain size properties changes the distribution of key macrofaunal species and how they interact with their environment, carrying potentially serious implications for the ecological functioning of these systems. Our knowledge of how benthic ecosystems respond to changes in sedimentary regimes is crucial to our ability to project and manage the impacts of environmental change. In this thesis, I investigated the multifaceted effects of increased sediment loading on the benthic biota and their functioning using natural and experimental sedimentary gradients.

An in situ experiment was conducted on an intertidal sandflat to examine the effects of short-term increases in suspended sediment concentration (SSC) on benthic autotrophic (primary production) and heterotrophic processes. In sunlit conditions, increases in SSC led to dramatic declines in net primary production and concomitant increases in NH₄⁺ efflux from the sediment to the water column. Although sediment chlorophyll-⍺ concentration increased with higher levels of SSC, a result that was likely a photoadaptive response to reduced light intensity, SSC reduced O₂ production per unit of chlorophyll -⍺ . SSC had no significant effect on sediment properties or heterotrophic processes such as sediment oxygen consumption or nutrient efflux, suggesting that temporary increases in suspended sediments (within the range of SSC tested) primarily affected photosynthetic processes. 

Sediment properties, macrofaunal diversity and biogeochemical fluxes were measured across natural gradients of silt and clay (hereafter mud) to determine the effects of habitat change associated with chronic sediment loading on the structure and functioning of benthic communities. There were significant declines in measures of macrofaunal diversity and the maximum densities of key bioturbating bivalves (Austrovenus stutchburyi and Macomona liliana) with increased mud content. Concurrently, the maximum rates of sediment oxygen consumption (SOC), NH₄⁺ efflux (a proxy of nutrient regeneration)and biomass standardised gross primary production (GPPChl-⍺) also decreased with increasing mud content. A. stutchburyi contributed disproportionately to variation in SOC and NH₄⁺ efflux, suggesting that losses of strongly interacting key species concomitant with increased sediment mud content could have a significant impact on ecosystem function. The results from this study demonstrate the significant loss of ecosystem function in intertidal sandflats that is likely from increased sediment mud content associated with long-term increases in sedimentation stress. 

The spatial distributions of MPB biomass, macrofaunal grazer abundances and deposit feeding activity were measured across a gradient of sediment mud content to determine relationships between grazers and MPB biomass across transitional sedimentary environments. The density of feeding traces produced by M. Liliana was measured as a proxy of deposit feeding activity by this species. MPB biomass was generally lower in areas with higher deposit feeding activity but this relationship was scale dependent, emerging over larger areas (tens of centimetres) but absent at local (centimetre) scales relative to the animal’s feeding ambit. Despite higher MPB biomass in muddy sediments, feeding trace density was markedly lower, suggesting lower feeding activity and trophic exchange in muddy compared with sandy sediments. The suspension feeding bivalve A. stutchburyi was positively associated with MPB biomass and the interaction between A. stutchburyi density and mud was the strongest predictor of MPB biomass. Thus, non-trophic interactions that potentially facilitate production may override the deleterious effects of grazing on MPB biomass by large macrofaunal species. This thesis demonstrates the high capacity of sandflat systems for primary, secondary production and nutrient regeneration and the degradation of these ecological properties and functions in muddier and more turbid systems. The decline in this functional capacity reflects the alterations of multiple ecological components and their interactions corresponding to habitat change. Defining changes in these interaction networks can improve our ability to track changes in ecosystem functioning and elucidate underlying pathways and potential mechanisms. In particular, this thesis highlights the value of observing changes in these ecological properties and functions across natural and experimental gradients at the appropriate scales in time and space over which stressors operate.

year: 2014

title: Māori men, relationships, and everyday practices: towards broadening domestic violence research

authors: King, Pita

subjects: Māori

abstract: Relationships are central to the health and wellbeing of Māori (indigenous people of New Zealand). Through processes of colonisation, cultural ways of relatedness embedded within Māori social structures experienced disruption and were reshaped over decades of assimilation. Māori knowledge and everyday practices that assisted in protecting Māori from societal problems, such as domestic violence, began to dwindle. In contemporary New Zealand, Māori are over-represented in domestic violence statistics. Utilising an auto-ethnographic approach and case studies, our research focuses on five Māori men’s experiences within intimate relationships and whānau (extended family) life. A significant feature of this research is that it provides insights into the ways Māori men draw on their cultural ways-of-being to enhance intimate relationships and maintain bonds within whānau and community life to forge new ways-of-being. Such insights have the potential to inform preventative measures against domestic violence within Māori communities.

text: Māori men, relationships, and everyday practices: towards broadening domestic violence research Relationships are central to the health and wellbeing of Māori (indigenous people of New Zealand). Through processes of colonisation, cultural ways of relatedness embedded within Māori social structures experienced disruption and were reshaped over decades of assimilation. Māori knowledge and everyday practices that assisted in protecting Māori from societal problems, such as domestic violence, began to dwindle. In contemporary New Zealand, Māori are over-represented in domestic violence statistics. Utilising an auto-ethnographic approach and case studies, our research focuses on five Māori men’s experiences within intimate relationships and whānau (extended family) life. A significant feature of this research is that it provides insights into the ways Māori men draw on their cultural ways-of-being to enhance intimate relationships and maintain bonds within whānau and community life to forge new ways-of-being. Such insights have the potential to inform preventative measures against domestic violence within Māori communities.

year: 2017