title: Minimising carbon emissions and energy expended for the New Zealand transport sector through to 2050

authors: Walmsley, Michael R.W.

abstract: Carbon Emissions Pinch Analysis (CEPA) and Energy Return on Energy Investment (EROI) analysis are combined to investigate the feasibility of New Zealand (NZ) reaching a 1990 emission levels for transport in 2050. The transportation sector traditionally has been a difficult area to transition to high levels of renewable energy because of the strong dependency on fossil fuels. Multiple scenarios for reducing transport emissions are analysed. With NZ’s unique mix of renewable energy resources the analysis demonstrates that NZ is in a very good position to sustainably meet their future transport needs provided substantial commitment is made to transition light vehicle fleet to hybrid vehicles, plug-in hybrids vehicles and electric vehicles by 2050. Electrification of rail within and between major centres will also require major political commitment. The resulting increase in electricity demand for transport is 3.6 TWh (or 4.8 % of electricity generation in NZ). We show the minimum amount of biofuel renewable production to achieve the goal of 1990 emissions level in 2050 is 46 PJ. Delivering 46 PJ is expected to be well within the potential biofuel production capacity of NZ. The delivery of economically competitive renewable liquid biofuels will also require close cooperation and system integration with other energy systems like the electricity sector and industrial process heat sector.

text: Minimising carbon emissions and energy expended for the New Zealand transport sector through to 2050 Carbon Emissions Pinch Analysis (CEPA) and Energy Return on Energy Investment (EROI) analysis are combined to investigate the feasibility of New Zealand (NZ) reaching a 1990 emission levels for transport in 2050. The transportation sector traditionally has been a difficult area to transition to high levels of renewable energy because of the strong dependency on fossil fuels. Multiple scenarios for reducing transport emissions are analysed. With NZ’s unique mix of renewable energy resources the analysis demonstrates that NZ is in a very good position to sustainably meet their future transport needs provided substantial commitment is made to transition light vehicle fleet to hybrid vehicles, plug-in hybrids vehicles and electric vehicles by 2050. Electrification of rail within and between major centres will also require major political commitment. The resulting increase in electricity demand for transport is 3.6 TWh (or 4.8 % of electricity generation in NZ). We show the minimum amount of biofuel renewable production to achieve the goal of 1990 emissions level in 2050 is 46 PJ. Delivering 46 PJ is expected to be well within the potential biofuel production capacity of NZ. The delivery of economically competitive renewable liquid biofuels will also require close cooperation and system integration with other energy systems like the electricity sector and industrial process heat sector.

year: 2014-09-20

title: Developmental gene expression profile of Vmo1 in the mouse auditory system

authors: Forrester-Gauntlett, Blaise Kelly Erin

abstract: Hearing loss (HL) is a sensory disorder that affects an estimated 250 million people worldwide and can greatly affect quality of life. In New Zealand, more than 10% of the population is affected by HL with the Māori population being overrepresented among all age groups. Therefore, understanding the mechanism of HL is extremely important for the development of new pharmaceuticals for the prevention or treatment of HL disorders.
The main aim of the research undertaken in this thesis was to characterise the function of the Mus musculus (mouse) vitelline membrane outer layer one (Vmo1) gene. This gene is considered an excellent candidate for being involved in human HL and/or balance disorders. Our hypothesis is based on its restricted gene localisation within the mouse inner ear and the postulated function of Reissner’s membrane.
Two methods were used to address this aim. Firstly, comparative genomics was used to determine the level of nucleotide and amino acid conservation of VMO1 across mammalian species, and to search for DNA motifs that may imply a biological function. Secondly, molecular biology and histochemical techniques were used to DNA sequence the Vmo1 gene, detect the expression of 22 kDa VMO1 protein within mouse tissues, and to localise the expression of VMO1 protein within the mouse inner ear.
Comparative genomics results showed VMO1 to be highly conserved across 36 species. An in-depth analysis of the differences and similiarites between the mouse, human and chicken indicated a high level of gene conservation with an even greater degree of identity and similarity seen at a proteomic level. In addition, a high level of conservation across amino acids involved in the formation and stabilisation of the three dimensional structure. Thus, results suggest an important function for the VMO1 protein.
Two commerical VMO1 antibodies were purchased to determine the localisation of the mouse VMO1 protein. They were validated for specificity using western blot analysis of protein lysates dissected from postnatal day 28 mice (P28). VMO1 was identified within the inner ear protein lysate and tear gland protein lysate of an expected molecular weight size of 20-37kDa with additional binding observed in the ear sample at 250kDa.
Immunohistochemistry detected high concentrations of VMO1 protein within the tectorial membrane (TM) and inner pillar cells (IPC) in inner ear sections from the mouse at P5. In agreement with the comparative genomics analysis, VMO1 is a secreted protein.
The movement of the hair cells (HC) relative to the TM is is essential for the transduction of sound into electrical signals. The IPC act as supporting cells for the hair cells, and help to couple movement of the basilar membrane to the HC. In conclusion, the importance of the TM and IPC in hearing function, and the localisation of the VMO1 protein within these structures implies an important role for VMO1 in hearing function. We recommend further studies to examine the specificity of the VMO1 antibody, and the development of a Vmo1 knockout mouse to support the functional analysis of Vmo1 in the auditory system.

text: Developmental gene expression profile of Vmo1 in the mouse auditory system Hearing loss (HL) is a sensory disorder that affects an estimated 250 million people worldwide and can greatly affect quality of life. In New Zealand, more than 10% of the population is affected by HL with the Māori population being overrepresented among all age groups. Therefore, understanding the mechanism of HL is extremely important for the development of new pharmaceuticals for the prevention or treatment of HL disorders.
The main aim of the research undertaken in this thesis was to characterise the function of the Mus musculus (mouse) vitelline membrane outer layer one (Vmo1) gene. This gene is considered an excellent candidate for being involved in human HL and/or balance disorders. Our hypothesis is based on its restricted gene localisation within the mouse inner ear and the postulated function of Reissner’s membrane.
Two methods were used to address this aim. Firstly, comparative genomics was used to determine the level of nucleotide and amino acid conservation of VMO1 across mammalian species, and to search for DNA motifs that may imply a biological function. Secondly, molecular biology and histochemical techniques were used to DNA sequence the Vmo1 gene, detect the expression of 22 kDa VMO1 protein within mouse tissues, and to localise the expression of VMO1 protein within the mouse inner ear.
Comparative genomics results showed VMO1 to be highly conserved across 36 species. An in-depth analysis of the differences and similiarites between the mouse, human and chicken indicated a high level of gene conservation with an even greater degree of identity and similarity seen at a proteomic level. In addition, a high level of conservation across amino acids involved in the formation and stabilisation of the three dimensional structure. Thus, results suggest an important function for the VMO1 protein.
Two commerical VMO1 antibodies were purchased to determine the localisation of the mouse VMO1 protein. They were validated for specificity using western blot analysis of protein lysates dissected from postnatal day 28 mice (P28). VMO1 was identified within the inner ear protein lysate and tear gland protein lysate of an expected molecular weight size of 20-37kDa with additional binding observed in the ear sample at 250kDa.
Immunohistochemistry detected high concentrations of VMO1 protein within the tectorial membrane (TM) and inner pillar cells (IPC) in inner ear sections from the mouse at P5. In agreement with the comparative genomics analysis, VMO1 is a secreted protein.
The movement of the hair cells (HC) relative to the TM is is essential for the transduction of sound into electrical signals. The IPC act as supporting cells for the hair cells, and help to couple movement of the basilar membrane to the HC. In conclusion, the importance of the TM and IPC in hearing function, and the localisation of the VMO1 protein within these structures implies an important role for VMO1 in hearing function. We recommend further studies to examine the specificity of the VMO1 antibody, and the development of a Vmo1 knockout mouse to support the functional analysis of Vmo1 in the auditory system.

year: 2013

title: Tū Māori Ake: Seeking which is above.

authors: Maxwell, Te Kahautu

abstract: Te Waka Āwhina 2015 Seeking that which is above Kaupapa - Philosophy o Aotearoa “Tū Māori ake” E ngā mana, e ngā reo, e ngā karangatanga, nei rā te powhiri ki a koutou katoa ‘MĀ TE MĀORI, MŌ TE MĀORI’ Te Waka Āwhina o Aotearoa National Training Hui by Māori for Māori within local government Āwhea: 2 – 4 February 2015 Ki whea: The Gallagher Academy of Performing Arts, The University of Waikato, Hamilton Hau kāinga: Te Puku o te Ika.

text: Tū Māori Ake: Seeking which is above. Te Waka Āwhina 2015 Seeking that which is above Kaupapa - Philosophy o Aotearoa “Tū Māori ake” E ngā mana, e ngā reo, e ngā karangatanga, nei rā te powhiri ki a koutou katoa ‘MĀ TE MĀORI, MŌ TE MĀORI’ Te Waka Āwhina o Aotearoa National Training Hui by Māori for Māori within local government Āwhea: 2 – 4 February 2015 Ki whea: The Gallagher Academy of Performing Arts, The University of Waikato, Hamilton Hau kāinga: Te Puku o te Ika.

year: 2015-02-03