title: A pepstatin-insensitive aspartic proteinase from a thermophilic Bacillus sp.

authors: Toogood, Helen S.

subjects: biology

abstract: Bacillus sp. strain Wp22.A1 produced a cell-associated aspartic proteinase which was purified to homogeneity using phenyl-Sepharose (hydrophobic and affinity chromatography) and Mono Q. The proteinase has a molecular mass of 45 kDa by SDS/PAGE and a pI of 3.8. It is insensitive to pepstatin, but is sensitive to the other aspartic proteinase-specific inhibitors diazoacetyl-DL-norleucine methyl ester (DAN) and 1,2-epoxy-3-(p-nitrophenoxy)propane. Inactivation by DAN was only partial, suggesting that it had non-specifically modified an aspartate residue at a site other than the active site. The enzyme was not inhibited by any of the serine or cysteine proteinase inhibitors tested. Maximum proteolytic activity was observed at pH 3.5. The proteinase had a higher activity with haemoglobin, but was more specific (Vmax./Km) for cytochrome c. Substrate inhibition was observed with both these substrates. The cleavage of oxidized insulin B chain tended to occur at sites where the P1 amino acid was bulky and non-polar, and the P1' amino acid was bulky and polar, such as its primary cleavage site of Val2-Asn3. The proteinase was stable in the pH range 2.5-5.5. Thermostability was increased in the presence of Ca2+, although to a lesser extent at higher temperatures. The thermostabilities at 60, 70, 80 and 90 degrees C were 45 h, 102, 21 and 3 min respectively in the presence of Ca2+.

text: A pepstatin-insensitive aspartic proteinase from a thermophilic Bacillus sp. Bacillus sp. strain Wp22.A1 produced a cell-associated aspartic proteinase which was purified to homogeneity using phenyl-Sepharose (hydrophobic and affinity chromatography) and Mono Q. The proteinase has a molecular mass of 45 kDa by SDS/PAGE and a pI of 3.8. It is insensitive to pepstatin, but is sensitive to the other aspartic proteinase-specific inhibitors diazoacetyl-DL-norleucine methyl ester (DAN) and 1,2-epoxy-3-(p-nitrophenoxy)propane. Inactivation by DAN was only partial, suggesting that it had non-specifically modified an aspartate residue at a site other than the active site. The enzyme was not inhibited by any of the serine or cysteine proteinase inhibitors tested. Maximum proteolytic activity was observed at pH 3.5. The proteinase had a higher activity with haemoglobin, but was more specific (Vmax./Km) for cytochrome c. Substrate inhibition was observed with both these substrates. The cleavage of oxidized insulin B chain tended to occur at sites where the P1 amino acid was bulky and non-polar, and the P1' amino acid was bulky and polar, such as its primary cleavage site of Val2-Asn3. The proteinase was stable in the pH range 2.5-5.5. Thermostability was increased in the presence of Ca2+, although to a lesser extent at higher temperatures. The thermostabilities at 60, 70, 80 and 90 degrees C were 45 h, 102, 21 and 3 min respectively in the presence of Ca2+.

year: 1995

title: Educational leadership….

authors: Strachan, Jane

subjects: educational leadership

abstract: An introduction is presented in which it discusses various reports within the issue on topics including the implications for leadership training, the exclusionary practices of educational leadership, and the standards for performance.

text: Educational leadership…. An introduction is presented in which it discusses various reports within the issue on topics including the implications for leadership training, the exclusionary practices of educational leadership, and the standards for performance.

year: 2000

title: Te mana o ngā toa: Māori experiences of Ngāti Tūmatauenga, the New Zealand Army

authors: Kohere, Cheyenne Atairehia Mere

subjects: Kaupapa Māori

abstract: Diverse realities impact Māori people and their capacity to flourish. It is well- established that Māori are significant contributors to the New Zealand Defence Force (NZDF), and especially the New Zealand Army. Noteworthy participation in major conflicts, especially WWI and WWII create a whakapapa of military tradition which contributed to institutional change within the Army in the 1990s. The inclusion of Māori cultural training in the New Zealand Army can contribute to morale and the effectiveness of the organisation (Hohaia, 2016). This master’s thesis aims to deepen understandings of the experiences of Māori personnel in the Army by exploring the role of the New Zealand Army in the health and wellbeing of Māori soldiers, as well as how the Army can be a career pathway for Māori and their whānau to flourish.
This research employs the use qualitative methods such as Kaupapa Māori theory, phenomenology, and pūrākau as a form of narrative analysis. Semi-structured interviews with five participants (three retired personnel and two currently serving) explores these factors that contribute to the flourishing of Māori soldiers in the New Zealand Army. Each pūrākau developed from interview data is an acknowledgment of the personal journeys of the five participants and contains philosophical thought, epistemological constructs, cultural codes, and worldviews (Lee, 2009).
The findings of the research highlight the complexities that arise when considering the health and wellbeing of Māori soldiers. A range of interconnected factors conceptualised within five themes contributes to participants’ ability to flourish. These five themes include hauora (health), whanaungatanga (relationships), manaakitanga (support), whanonga pono (values) and whakapāwera (hardships). These results suggest that flourishing for Māori personnel is complex, and there is not a simple route to enable Māori to flourish. There are many ‘trade-offs’ for Māori and their experiences. However, Māori service provides opportunities and obstacles for an individual, as well as their whānau, hapū and iwi.

text: Te mana o ngā toa: Māori experiences of Ngāti Tūmatauenga, the New Zealand Army Diverse realities impact Māori people and their capacity to flourish. It is well- established that Māori are significant contributors to the New Zealand Defence Force (NZDF), and especially the New Zealand Army. Noteworthy participation in major conflicts, especially WWI and WWII create a whakapapa of military tradition which contributed to institutional change within the Army in the 1990s. The inclusion of Māori cultural training in the New Zealand Army can contribute to morale and the effectiveness of the organisation (Hohaia, 2016). This master’s thesis aims to deepen understandings of the experiences of Māori personnel in the Army by exploring the role of the New Zealand Army in the health and wellbeing of Māori soldiers, as well as how the Army can be a career pathway for Māori and their whānau to flourish.
This research employs the use qualitative methods such as Kaupapa Māori theory, phenomenology, and pūrākau as a form of narrative analysis. Semi-structured interviews with five participants (three retired personnel and two currently serving) explores these factors that contribute to the flourishing of Māori soldiers in the New Zealand Army. Each pūrākau developed from interview data is an acknowledgment of the personal journeys of the five participants and contains philosophical thought, epistemological constructs, cultural codes, and worldviews (Lee, 2009).
The findings of the research highlight the complexities that arise when considering the health and wellbeing of Māori soldiers. A range of interconnected factors conceptualised within five themes contributes to participants’ ability to flourish. These five themes include hauora (health), whanaungatanga (relationships), manaakitanga (support), whanonga pono (values) and whakapāwera (hardships). These results suggest that flourishing for Māori personnel is complex, and there is not a simple route to enable Māori to flourish. There are many ‘trade-offs’ for Māori and their experiences. However, Māori service provides opportunities and obstacles for an individual, as well as their whānau, hapū and iwi.

year: 2022