title: Light limitation and water velocity modify the impacts of simulated marine heatwaves on juvenile giant kelp.

authors: Bunting, Imogen

subjects: coastal darkening

abstract: Coastal regions are complex habitats, where multiple natural and anthropogenic drivers can interact to affect the survival and growth of marine organisms. The giant kelp Macrocystis pyrifera is sensitive to increasing seawater temperatures and susceptible to marine heatwaves. Light availability and hydrodynamics can also affect the growth, morphology, and resilience of this species. In this experiment, juvenile sporophytes of M. pyrifera from Scorching Bay, Wellington, Aotearoa, New Zealand, a were exposed to a combination of simulated marine heatwaves at one of four different temperatures (20, 22, and 24°C compared to a 16°C control), one of two irradiance levels (shaded: 0.9 mol photons · m−2 · d−1 or ambient: 1.4 mol photons · m−2 · d−1), and one of two flow speeds (5.3 cm · s−1 or 6.1 cm · s−1) in a fully factorial design. Simulated heatwaves lasted for 21 days, with temperatures ramped by 2°C · d−1, followed by a 21-day recovery phase. The heatwave treatments represented severe heatwaves in present day or hypothetical future conditions, whereas the control represented historical average summer sea temperatures in Wellington, and 21 days represented a realistic duration for heatwaves in this region. Temperature was the main driver of negative physiological impacts, with 100% of sporophytes dying within 42 days of exposure to a 24°C heatwave. Sporophytes experienced 44% mortality at 20°C and 81% mortality at 22°C, and growth rates declined significantly with increasing temperature. However, survival rates were modified by light and water velocity, with 56% of sporophytes surviving under a combination of ambient light and fast water velocity, compared with less than 50% under each of the other light-velocity combinations. Light limitation also reduced sporophyte survival, growth rates, and effective quantum yield. Water velocity alone did not significantly affect sporophytes, but flow speeds had interactive effects with temperature and light. The findings of this experiment suggest that M. pyrifera at sites with optimal environmental conditions, including low sediment loads and fast tidal flows, could be more resilient to marine heatwaves, as long as temperatures do not exceed critical thresholds for survival.

text: Light limitation and water velocity modify the impacts of simulated marine heatwaves on juvenile giant kelp. Coastal regions are complex habitats, where multiple natural and anthropogenic drivers can interact to affect the survival and growth of marine organisms. The giant kelp Macrocystis pyrifera is sensitive to increasing seawater temperatures and susceptible to marine heatwaves. Light availability and hydrodynamics can also affect the growth, morphology, and resilience of this species. In this experiment, juvenile sporophytes of M. pyrifera from Scorching Bay, Wellington, Aotearoa, New Zealand, a were exposed to a combination of simulated marine heatwaves at one of four different temperatures (20, 22, and 24°C compared to a 16°C control), one of two irradiance levels (shaded: 0.9 mol photons · m−2 · d−1 or ambient: 1.4 mol photons · m−2 · d−1), and one of two flow speeds (5.3 cm · s−1 or 6.1 cm · s−1) in a fully factorial design. Simulated heatwaves lasted for 21 days, with temperatures ramped by 2°C · d−1, followed by a 21-day recovery phase. The heatwave treatments represented severe heatwaves in present day or hypothetical future conditions, whereas the control represented historical average summer sea temperatures in Wellington, and 21 days represented a realistic duration for heatwaves in this region. Temperature was the main driver of negative physiological impacts, with 100% of sporophytes dying within 42 days of exposure to a 24°C heatwave. Sporophytes experienced 44% mortality at 20°C and 81% mortality at 22°C, and growth rates declined significantly with increasing temperature. However, survival rates were modified by light and water velocity, with 56% of sporophytes surviving under a combination of ambient light and fast water velocity, compared with less than 50% under each of the other light-velocity combinations. Light limitation also reduced sporophyte survival, growth rates, and effective quantum yield. Water velocity alone did not significantly affect sporophytes, but flow speeds had interactive effects with temperature and light. The findings of this experiment suggest that M. pyrifera at sites with optimal environmental conditions, including low sediment loads and fast tidal flows, could be more resilient to marine heatwaves, as long as temperatures do not exceed critical thresholds for survival.

year: 2025

title: Flow cytometric characterization of freshwater crayfish hemocytes for the examination of physiological status in wild and captive animals

authors: Taylor, Sean

subjects: New Zealand

abstract: Enumeration of invertebrate hemocytes is a potentially powerful tool for the determination of physiological effects of extrinsic stressors, such as hypoxia, disease, and toxicant exposure. A detailed flow cytometric method of broad application was developed for the objective characterization and enumeration of the hemocytes of New Zealand freshwater crayfish Paranephrops planifrons for the purpose of physiological health assessment. Hemocyte populations were isolated by flow cytometric sorting based on differential light scatter properties followed by morphological characterization via light microscopy and software image analysis. Cells were identified as hyaline, semigranular, and granular hemocytes based on established invertebrate hemocyte classification. A characteristic decrease in nuclear size, an increase in granularity between the hyaline and granular cells, and the eccentric location of nuclei in granular cells were also observed. The granulocyte subpopulations were observed to possess varying degrees of granularity. The developed methodology was used to perform total and differential hemocyte counts from three lake populations and between wild and captive crayfish specimens. Differences in total and differential hemocyte counts were not observed among the wild populations. However, specimens held in captivity for 14 d exhibited a significant 63% reduction in total hemocyte count, whereas the relative hemocyte proportions remained the same. These results demonstrate the utility of this method for the investigation of subacute stressor effects in selected decapod crustaceans.

text: Flow cytometric characterization of freshwater crayfish hemocytes for the examination of physiological status in wild and captive animals Enumeration of invertebrate hemocytes is a potentially powerful tool for the determination of physiological effects of extrinsic stressors, such as hypoxia, disease, and toxicant exposure. A detailed flow cytometric method of broad application was developed for the objective characterization and enumeration of the hemocytes of New Zealand freshwater crayfish Paranephrops planifrons for the purpose of physiological health assessment. Hemocyte populations were isolated by flow cytometric sorting based on differential light scatter properties followed by morphological characterization via light microscopy and software image analysis. Cells were identified as hyaline, semigranular, and granular hemocytes based on established invertebrate hemocyte classification. A characteristic decrease in nuclear size, an increase in granularity between the hyaline and granular cells, and the eccentric location of nuclei in granular cells were also observed. The granulocyte subpopulations were observed to possess varying degrees of granularity. The developed methodology was used to perform total and differential hemocyte counts from three lake populations and between wild and captive crayfish specimens. Differences in total and differential hemocyte counts were not observed among the wild populations. However, specimens held in captivity for 14 d exhibited a significant 63% reduction in total hemocyte count, whereas the relative hemocyte proportions remained the same. These results demonstrate the utility of this method for the investigation of subacute stressor effects in selected decapod crustaceans.

year: 2009

title: Cultural justice and ethics: From within

authors: Garner, Kerin

subjects: Maori

abstract: Where I eventually got a lot of support, awhi, aroha, was from the PPTA when I became a member of Te Huarahi and they helped to see me through the tears and the confusion. It wasn’t because I was Maori, it was because I was a human being. I think the process I have gone through is a process of becoming human, and becoming real in thinking; yes, my experience is valid. I’m not just Maori, I’m Pakeha too. I want both of them, I am both of them. I don’t have to get up and speak fluent Maori right now. Perhaps it will come. I hope it does because I want it to, and I’m going to work on it. But I do feel that people like me, and there are many of us, are not marginal. We are bridges between cultures. This diversity has to be acknowledged, honoured and respected.

text: Cultural justice and ethics: From within Where I eventually got a lot of support, awhi, aroha, was from the PPTA when I became a member of Te Huarahi and they helped to see me through the tears and the confusion. It wasn’t because I was Maori, it was because I was a human being. I think the process I have gone through is a process of becoming human, and becoming real in thinking; yes, my experience is valid. I’m not just Maori, I’m Pakeha too. I want both of them, I am both of them. I don’t have to get up and speak fluent Maori right now. Perhaps it will come. I hope it does because I want it to, and I’m going to work on it. But I do feel that people like me, and there are many of us, are not marginal. We are bridges between cultures. This diversity has to be acknowledged, honoured and respected.

year: 1993