title: Thermorudis pharmacophila sp nov., a novel member of the class Thermomicrobia isolated from geothermal soil, and emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilus

authors: Houghton, Karen M.

subjects: Science & Technology

abstract: An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2T grew at 53–76 °C and at pH 5.9–8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C18 : 0 and C18 : 0. Polar lipids were all linked to long-chain 1,2-diols, and comprised 2-acylalkyldiol-1-O-phosphoinositol (diolPI), 2-acylalkyldiol-1-O-phosphoacylmannoside (diolP-acylMan), 2-acylalkyldiol-1-O-phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1-O-phosphoinositol mannoside (diolPI-Man). Strain WKT50.2T utilized a range of cellulosic substrates, alcohols and organic acids for growth, but was unable to utilize monosaccharides. Robust growth of WKT50.2T was observed on protein derivatives. WKT50.2T was sensitive to ampicillin, chloramphenicol, kanamycin, neomycin, polymyxin B, streptomycin and vancomycin. Metronidazole, lasalocid A and trimethoprim stimulated growth. Phylogenetic analysis of 16S rRNA gene sequences showed that WKT50.2T belonged to the class Thermomicrobia within the phylum Chloroflexi, and was most closely related to Thermorudis peleae KI4T (99.6% similarity). DNA–DNA hybridization between WKT50.2T and Thermorudis peleae DSM 27169T was 18.0%. Physiological and biochemical tests confirmed the phenotypic and genotypic differentiation of strain WKT50.2T from Thermorudis peleae KI4T and other members of the Thermomicrobia. On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain WKT50.2T represents a novel species, for which the name Thermorudis pharmacophila sp. nov. is proposed, with the type strain WKT50.2T ( = DSM 26011T = ICMP 20042T). Emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilus are also proposed, and include the description of a novel respiratory quinone, MK-8 2,3-epoxide (23%), in Thermomicrobium roseum.

text: Thermorudis pharmacophila sp nov., a novel member of the class Thermomicrobia isolated from geothermal soil, and emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilus An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2T grew at 53–76 °C and at pH 5.9–8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C18 : 0 and C18 : 0. Polar lipids were all linked to long-chain 1,2-diols, and comprised 2-acylalkyldiol-1-O-phosphoinositol (diolPI), 2-acylalkyldiol-1-O-phosphoacylmannoside (diolP-acylMan), 2-acylalkyldiol-1-O-phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1-O-phosphoinositol mannoside (diolPI-Man). Strain WKT50.2T utilized a range of cellulosic substrates, alcohols and organic acids for growth, but was unable to utilize monosaccharides. Robust growth of WKT50.2T was observed on protein derivatives. WKT50.2T was sensitive to ampicillin, chloramphenicol, kanamycin, neomycin, polymyxin B, streptomycin and vancomycin. Metronidazole, lasalocid A and trimethoprim stimulated growth. Phylogenetic analysis of 16S rRNA gene sequences showed that WKT50.2T belonged to the class Thermomicrobia within the phylum Chloroflexi, and was most closely related to Thermorudis peleae KI4T (99.6% similarity). DNA–DNA hybridization between WKT50.2T and Thermorudis peleae DSM 27169T was 18.0%. Physiological and biochemical tests confirmed the phenotypic and genotypic differentiation of strain WKT50.2T from Thermorudis peleae KI4T and other members of the Thermomicrobia. On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain WKT50.2T represents a novel species, for which the name Thermorudis pharmacophila sp. nov. is proposed, with the type strain WKT50.2T ( = DSM 26011T = ICMP 20042T). Emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilus are also proposed, and include the description of a novel respiratory quinone, MK-8 2,3-epoxide (23%), in Thermomicrobium roseum.

year: 2015

title: Environmental offsets and water resource opportunities with Lake Onslow pumped storage

authors: Karaminik, Yasaman Maryam

abstract: As part of its transition toward an aspirational goal of 100% renewable electricity generation, New Zealand had been investigating an option of replacing the need for dry-year fossil fuel power generation with the world’s largest pumped storage scheme by energy storage measure. The scheme was to be located at Lake Onslow in the southern South Island. It would have an energy storage capacity up to 5 TWh for a construction cost of 16 billion dollars. However, the 2023 change of government means the scheme is not presently under active evaluation. Evaluation may well be restarted with a subsequent change of government, depending on party policy. 
While aiding emission reduction, there would be local environmental impact from expanding the existing Lake Onslow reservoir, which will flood over nearby wetlands. 
This study considers a range of potential environmental mitigation measures that might counterbalance the scheme's ecological impact. Consideration is also given to the large water volume in the Onslow reservoir potentially able to act as insurance against future extreme regional droughts that may arise from climate change.
The possible offsets include the restoration of two existing scenic hydro storage lakes – Lake Monowai and Lake Hawea. An argument is made that the large amount of new hydro storage capacity at Lake Onslow means that the relatively small storage contributions of these two lakes no longer justifies their inundation environmental impact. This would start a change in national thinking because to date no hydro storage lake in New Zealand has been restored for purely environmental reasons. 
In addition to lake restoration, discussion is also presented about the possibility of a hypothetical partial flow restoration of the depleted Waiau River. It was New Zealand’s second largest river by discharge prior to diversion into the Manapouri power station. It is tentatively suggested that new wind power in Southland buffered by Onslow pumped storage would enable partial closure of the Manapouri station, restoring about half of the original flow the lower Waiau River downstream of Lake Manapouri. 
Onslow water storage would involve cubic kilometres of water, so some water could be used to offset extreme droughts without impeding pumped storage energy functionality. A narrow-diameter rock tunnel could also be constructed between the expanded Lake Onslow and the Dunedin city water intake at Deep Creek. This would make Dunedin drought-resilient against climate change because presently the city water supply is derived just from small local catchments with little storage.
Some Onslow water might also be used to maintain water flow of the eastern Otago Taieri River in an extreme drought. However, this would raise cultural and ecological issues that would need to be considered prior to any water release.

text: Environmental offsets and water resource opportunities with Lake Onslow pumped storage As part of its transition toward an aspirational goal of 100% renewable electricity generation, New Zealand had been investigating an option of replacing the need for dry-year fossil fuel power generation with the world’s largest pumped storage scheme by energy storage measure. The scheme was to be located at Lake Onslow in the southern South Island. It would have an energy storage capacity up to 5 TWh for a construction cost of 16 billion dollars. However, the 2023 change of government means the scheme is not presently under active evaluation. Evaluation may well be restarted with a subsequent change of government, depending on party policy. 
While aiding emission reduction, there would be local environmental impact from expanding the existing Lake Onslow reservoir, which will flood over nearby wetlands. 
This study considers a range of potential environmental mitigation measures that might counterbalance the scheme's ecological impact. Consideration is also given to the large water volume in the Onslow reservoir potentially able to act as insurance against future extreme regional droughts that may arise from climate change.
The possible offsets include the restoration of two existing scenic hydro storage lakes – Lake Monowai and Lake Hawea. An argument is made that the large amount of new hydro storage capacity at Lake Onslow means that the relatively small storage contributions of these two lakes no longer justifies their inundation environmental impact. This would start a change in national thinking because to date no hydro storage lake in New Zealand has been restored for purely environmental reasons. 
In addition to lake restoration, discussion is also presented about the possibility of a hypothetical partial flow restoration of the depleted Waiau River. It was New Zealand’s second largest river by discharge prior to diversion into the Manapouri power station. It is tentatively suggested that new wind power in Southland buffered by Onslow pumped storage would enable partial closure of the Manapouri station, restoring about half of the original flow the lower Waiau River downstream of Lake Manapouri. 
Onslow water storage would involve cubic kilometres of water, so some water could be used to offset extreme droughts without impeding pumped storage energy functionality. A narrow-diameter rock tunnel could also be constructed between the expanded Lake Onslow and the Dunedin city water intake at Deep Creek. This would make Dunedin drought-resilient against climate change because presently the city water supply is derived just from small local catchments with little storage.
Some Onslow water might also be used to maintain water flow of the eastern Otago Taieri River in an extreme drought. However, this would raise cultural and ecological issues that would need to be considered prior to any water release.

year: 2024-03-18

title: Planning and governance under the LGA: Lessons from the RMA experience.

authors: Borrie, Nancy

subjects: New Zealand

abstract: The purpose of this report is to identify ways in which experiences gained from the
RMA as a devolved and co-operative planning mandate can enable local and central
government and other stakeholders to more effectively implement the LGA. The
report is based on findings from the FRST-funded research programme on Planning
under Co-operative Mandates (PUCM). We argue in this report that the experiences gained from the RMA can inform
effective implementation of the LGA in three important respects: Preparation and implementation of LTCCPs; The community consultation process for formulating community outcomes;
and Māori participation in planning and governance.

text: Planning and governance under the LGA: Lessons from the RMA experience. The purpose of this report is to identify ways in which experiences gained from the
RMA as a devolved and co-operative planning mandate can enable local and central
government and other stakeholders to more effectively implement the LGA. The
report is based on findings from the FRST-funded research programme on Planning
under Co-operative Mandates (PUCM). We argue in this report that the experiences gained from the RMA can inform
effective implementation of the LGA in three important respects: Preparation and implementation of LTCCPs; The community consultation process for formulating community outcomes;
and Māori participation in planning and governance.

year: 2004-06-01