title: Diagnostic dilemmas associated with the pinnacle IN vaccine

authors: Cursons, Raymond T.

subjects: Strangles, infection, live vaccine next generation sequencing

abstract: The development of clinical signs of strangles in horses following immunization with a live attenuated vaccine can present a number of diagnostic challenges. Foremost from an epidemiological perspective it is important to ascertain if the infection was due solely to the modified live vaccine, reversion of the vaccine strain to wild-type, recombination between vaccine and wild-type strains or to recent infection with a wild-type strain. To answer these diagnostics challenges we utilized next generation sequencing to confirm that two cases of strangles in previously healthy yearling horses were caused by the vaccine strain. Both isolates recovered from the two cases of vaccine-related strangles contained 67 of the 68 mutations specific for the Pinnacle vaccine. Our data shed new light on the safety of this vaccine and suggest that factors beyond the maturity of the animal’s immune system can influence the development of post-vaccination adverse reactions in some horses

text: Diagnostic dilemmas associated with the pinnacle IN vaccine The development of clinical signs of strangles in horses following immunization with a live attenuated vaccine can present a number of diagnostic challenges. Foremost from an epidemiological perspective it is important to ascertain if the infection was due solely to the modified live vaccine, reversion of the vaccine strain to wild-type, recombination between vaccine and wild-type strains or to recent infection with a wild-type strain. To answer these diagnostics challenges we utilized next generation sequencing to confirm that two cases of strangles in previously healthy yearling horses were caused by the vaccine strain. Both isolates recovered from the two cases of vaccine-related strangles contained 67 of the 68 mutations specific for the Pinnacle vaccine. Our data shed new light on the safety of this vaccine and suggest that factors beyond the maturity of the animal’s immune system can influence the development of post-vaccination adverse reactions in some horses

year: 2015

title: SIFTing the relevant from the irrelevant: Automatically detecting objects in training images

authors: Zhang, Edmond Yiwen

subjects: Image processing

abstract: Many state-of-the-art object recognition systems rely on identifying the location of objects in images, in order to better learn its visual attributes. In this paper, we propose four simple yet powerful hybrid ROI detection methods (combining both local and global features), based on frequently occurring keypoints. We show that our methods demonstrate competitive performance in two different types of datasets, the Caltech101 dataset and the GRAZ-02 dataset, where the pairs of keypoint bounding box method achieved the best accuracies overall.

text: SIFTing the relevant from the irrelevant: Automatically detecting objects in training images Many state-of-the-art object recognition systems rely on identifying the location of objects in images, in order to better learn its visual attributes. In this paper, we propose four simple yet powerful hybrid ROI detection methods (combining both local and global features), based on frequently occurring keypoints. We show that our methods demonstrate competitive performance in two different types of datasets, the Caltech101 dataset and the GRAZ-02 dataset, where the pairs of keypoint bounding box method achieved the best accuracies overall.

year: 2009

title: Kūkupa, koro, and kai: The use of Māori vocabulary items in New Zealand English children's picture books

authors: Daly, Nicola

subjects: New Zealand

abstract: When a linguistic form from one language is used in another language, such words are known as borrowings or loan words (Crystal 2003: 56). The English language is renowned for its large capacity for borrowing and it has been suggested that the growth in internationalism in recent times has led to people seeking new words to indicate their local identity (Crystal 1995; Crystal 2003). Certainly this is the case of New Zealand English, the most distinctive aspect of which is borrowings from te reo Maori (Deverson 1991). In 1984 Deverson estimated that most New Zealanders have a passive knowledge of at least 40-50 borrowed Maori loan words (Deverson 1984). This figure has been recently revised by Macalister to 70-80 such words (20,-,63.). A study of the frequency of Maori loan words in New Zealand English in New Zealand School Journals of the 1960s and 1990s showed an incidence of around 6 words per 1,000 (Macalister 1999). Kennedy and Yamazaki (1999) also found borrowed Maori words at a rate of 6 per thousand words. Macalister (2006b) has examined the use of Maori loan words in New Zealand English across a 150 year period from 1850-2000. He examined a corpus of a little under five and a half million words from three sources: Newspapers, parliamentary debates and School Journals. Across the three sources there was an increase from 3.29 words per 1,000 in 1850 to 8.8 per thousand in 2000. Macalister lists the reasons for this change as including urbanisation of the Maori population between 1945 and 1975, which created more contact; the changing status of the Maori language with the kOhanga reo movement; the establishment of the Maori Language Commission (Te Taura Whiri i te Reo Maori) and legislation of Maori as an official language of New Zealand in 1987.

text: Kūkupa, koro, and kai: The use of Māori vocabulary items in New Zealand English children's picture books When a linguistic form from one language is used in another language, such words are known as borrowings or loan words (Crystal 2003: 56). The English language is renowned for its large capacity for borrowing and it has been suggested that the growth in internationalism in recent times has led to people seeking new words to indicate their local identity (Crystal 1995; Crystal 2003). Certainly this is the case of New Zealand English, the most distinctive aspect of which is borrowings from te reo Maori (Deverson 1991). In 1984 Deverson estimated that most New Zealanders have a passive knowledge of at least 40-50 borrowed Maori loan words (Deverson 1984). This figure has been recently revised by Macalister to 70-80 such words (20,-,63.). A study of the frequency of Maori loan words in New Zealand English in New Zealand School Journals of the 1960s and 1990s showed an incidence of around 6 words per 1,000 (Macalister 1999). Kennedy and Yamazaki (1999) also found borrowed Maori words at a rate of 6 per thousand words. Macalister (2006b) has examined the use of Maori loan words in New Zealand English across a 150 year period from 1850-2000. He examined a corpus of a little under five and a half million words from three sources: Newspapers, parliamentary debates and School Journals. Across the three sources there was an increase from 3.29 words per 1,000 in 1850 to 8.8 per thousand in 2000. Macalister lists the reasons for this change as including urbanisation of the Maori population between 1945 and 1975, which created more contact; the changing status of the Maori language with the kOhanga reo movement; the establishment of the Maori Language Commission (Te Taura Whiri i te Reo Maori) and legislation of Maori as an official language of New Zealand in 1987.

year: 2007