title: No evidence for a culturable bacterial tetrodotoxin producer in Pleurobranchaea maculata (Gastropoda: Pleurobranchidae) and Stylochoplana sp. (Platyhelminthes: Polycladida)

authors: Salvitti, Lauren R.

subjects: bacteria

abstract: Tetrodotoxin (TTX) is a potent neurotoxin found in the tissues of many taxonomically diverse organisms. Its origin has been the topic of much debate, with suggestions including endogenous production, acquisition through diet, and symbiotic bacterial synthesis. Bacterial production of TTX has been reported in isolates from marine biota, but at lower than expected concentrations. In this study, 102 strains were isolated from Pleurobranchaea maculata (Opisthobranchia) and Stylochoplana sp. (Platyhelminthes). Tetrodotoxin production was tested utilizing a recently developed sensitive method to detect the C9 base of TTX via liquid chromatography—mass spectrometry. Bacterial strains were characterized by sequencing a region of the 16S ribosomal RNA gene. To account for the possibility that TTX is produced by a consortium of bacteria, a series of experiments using marine broth spiked with various P. maculata tissues were undertaken. Sixteen unique strains from P. maculata and one from Stylochoplana sp. were isolated, representing eight different genera; Pseudomonadales, Actinomycetales, Oceanospirillales, Thiotrichales, Rhodobacterales, Sphingomonadales, Bacillales, and Vibrionales. Molecular fingerprinting of bacterial communities from broth experiments showed little change over the first four days. No C9 base or TTX was detected in isolates or broth experiments (past day 0), suggesting a culturable microbial source of TTX in P. maculata and Stylochoplana sp. is unlikely.

text: No evidence for a culturable bacterial tetrodotoxin producer in Pleurobranchaea maculata (Gastropoda: Pleurobranchidae) and Stylochoplana sp. (Platyhelminthes: Polycladida) Tetrodotoxin (TTX) is a potent neurotoxin found in the tissues of many taxonomically diverse organisms. Its origin has been the topic of much debate, with suggestions including endogenous production, acquisition through diet, and symbiotic bacterial synthesis. Bacterial production of TTX has been reported in isolates from marine biota, but at lower than expected concentrations. In this study, 102 strains were isolated from Pleurobranchaea maculata (Opisthobranchia) and Stylochoplana sp. (Platyhelminthes). Tetrodotoxin production was tested utilizing a recently developed sensitive method to detect the C9 base of TTX via liquid chromatography—mass spectrometry. Bacterial strains were characterized by sequencing a region of the 16S ribosomal RNA gene. To account for the possibility that TTX is produced by a consortium of bacteria, a series of experiments using marine broth spiked with various P. maculata tissues were undertaken. Sixteen unique strains from P. maculata and one from Stylochoplana sp. were isolated, representing eight different genera; Pseudomonadales, Actinomycetales, Oceanospirillales, Thiotrichales, Rhodobacterales, Sphingomonadales, Bacillales, and Vibrionales. Molecular fingerprinting of bacterial communities from broth experiments showed little change over the first four days. No C9 base or TTX was detected in isolates or broth experiments (past day 0), suggesting a culturable microbial source of TTX in P. maculata and Stylochoplana sp. is unlikely.

year: 2015-01-28

title: Contributions to technology education through funds of knowledge

authors: Fox-Turnbull, Wendy

subjects: technology education

abstract: The value and place of cultural knowledge, particularly that of minority groups, is frequently overlooked in school settings. However, when teachers are aware of students’ backgrounds, and cultural practices, the likelihood of students making use of this knowledge increases. This article reports on a study that explored the contribution of students’ Funds of Knowledge in undertaking technological activity. It explores the Funds of Knowledge deployed in technological practice and the role these played when students collaboratively developed their technological outcomes.


Set in a primary school with six and ten year old students, this study revealed that students deployed home and community knowledge when developing technological outcomes. The article introduces two sub-categories of Funds of Knowledge and compares the use of Funds of Knowledge between the two year levels and across a unit of work.


This study highlights the use of Funds of Knowledge in technology education and examines aspects within Funds of Knowledge that are applicable to it.

text: Contributions to technology education through funds of knowledge The value and place of cultural knowledge, particularly that of minority groups, is frequently overlooked in school settings. However, when teachers are aware of students’ backgrounds, and cultural practices, the likelihood of students making use of this knowledge increases. This article reports on a study that explored the contribution of students’ Funds of Knowledge in undertaking technological activity. It explores the Funds of Knowledge deployed in technological practice and the role these played when students collaboratively developed their technological outcomes.


Set in a primary school with six and ten year old students, this study revealed that students deployed home and community knowledge when developing technological outcomes. The article introduces two sub-categories of Funds of Knowledge and compares the use of Funds of Knowledge between the two year levels and across a unit of work.


This study highlights the use of Funds of Knowledge in technology education and examines aspects within Funds of Knowledge that are applicable to it.

year: 2015

title: Kaupapa Māori framework and literature review of key prinicples

authors: Kennedy, Nathan

subjects: New Zealand

abstract: The literature review in this report was the starting point for the development of a Māori research strand within the Planning Under Co-operative Mandates (PUCM) research programme. The original purpose of this report Kaupapa Māori Framework and Literature Review of Key Principles was to establish definitions of environmentally significant concepts of kaupapa and tikanga Māori. In  addition,  the  review  sought  to  identify  and  briefly  describe  significant  variations between  understandings of the key concepts without attempting to reconcile these. As the purpose of the review in 2005 was to inform the development of a kaupapa Māori methodology for the identification and development of Māori environmental outcomes and indicators, we paid particular regard to Māori perceptions of the environment and the relevance of each concept in environmental terms.

text: Kaupapa Māori framework and literature review of key prinicples The literature review in this report was the starting point for the development of a Māori research strand within the Planning Under Co-operative Mandates (PUCM) research programme. The original purpose of this report Kaupapa Māori Framework and Literature Review of Key Principles was to establish definitions of environmentally significant concepts of kaupapa and tikanga Māori. In  addition,  the  review  sought  to  identify  and  briefly  describe  significant  variations between  understandings of the key concepts without attempting to reconcile these. As the purpose of the review in 2005 was to inform the development of a kaupapa Māori methodology for the identification and development of Māori environmental outcomes and indicators, we paid particular regard to Māori perceptions of the environment and the relevance of each concept in environmental terms.

year: 2009-06-30