title: The factors responsible for the varying levels of UMF® in mānuka (Leptospermum scoparium) honey

authors: Stephens, Jonathan McDonald Counsell

subjects: Leptospermum scoparium

abstract: The variability in the level of the non-peroxide antibacterial component (UMF®) of mānuka honey produced in New Zealand was studied. A field analysis confirmed considerable variability existed in the honeys, and a number of hypotheses to explain this variability were proposed and examined.

Nectar derived from Leptospermum scoparium (mānuka), was confirmed to be the
source of UMF®.

The dilution of mānuka honey with nectar derived from other floral sources was found to proportionally reduce the UMF® in monofloral mānuka honey. The utilisation of the thixotropic properties of mānuka honey allowed the degree of dilution in the field samples to be established, and an adjustment of the field results to account for the dilution of UMF® by other honey types revealed all monofloral mānuka honey contains UMF®. However, in the monofloral mānuka honey, significantly different levels of UMF® activity were found to come from reasonably well-defined geographic regions.

The cause of the variable levels of UMF® activity in mānuka honey would appear to be the different varieties of L. scoparium being harvested by the honeybees, and the environmental parameters influencing nectar production or another species interacting with L. scoparium do not appear to influence UMF® activity.

Three methods were used to establish genetic variability within regions of the North Island of New Zealand that gave rise to the various levels of UMF® activity. Analyses of morphological characteristics, chemotaxonomic essential oil profiles, and population genetics of L. scoparium populations were conducted, and the conclusions that were drawn from each of these were very similar. Two major divisions were identified, each divided into two varieties. The northern division, which contained the core populations from Northland and Waikato, represented the previously described L. scoparium var. incanum and L. scoparium var. linifolium. This division yielded mānuka honey with high UMF® activity. The southern division, which contained the core populations from the Central North Island and East Coast, represented the previously described L. scoparium var. myrtifolium and an unnamed variety. The latter, growing principally on the East Coast, uniquely contains triketones essential oils. The southern division yielded mānuka honey with low UMF® activity. Hybridisation between these varieties will occur, leading to a continuum of UMF® activity in mānuka honey.

The data indicated multiple dispersions of L. scoparium to New Zealand from the evolutionary centre of the persistent-capsule Leptospermum group in south-east Australia, and later regional dispersal in New Zealand.

From this study two hypotheses were accepted: the variability in the UMF® activity of mānuka honey is due to both the dilution of mānuka honey by other honey types and the variety of L. scoparium harvested.

text: The factors responsible for the varying levels of UMF® in mānuka (Leptospermum scoparium) honey The variability in the level of the non-peroxide antibacterial component (UMF®) of mānuka honey produced in New Zealand was studied. A field analysis confirmed considerable variability existed in the honeys, and a number of hypotheses to explain this variability were proposed and examined.

Nectar derived from Leptospermum scoparium (mānuka), was confirmed to be the
source of UMF®.

The dilution of mānuka honey with nectar derived from other floral sources was found to proportionally reduce the UMF® in monofloral mānuka honey. The utilisation of the thixotropic properties of mānuka honey allowed the degree of dilution in the field samples to be established, and an adjustment of the field results to account for the dilution of UMF® by other honey types revealed all monofloral mānuka honey contains UMF®. However, in the monofloral mānuka honey, significantly different levels of UMF® activity were found to come from reasonably well-defined geographic regions.

The cause of the variable levels of UMF® activity in mānuka honey would appear to be the different varieties of L. scoparium being harvested by the honeybees, and the environmental parameters influencing nectar production or another species interacting with L. scoparium do not appear to influence UMF® activity.

Three methods were used to establish genetic variability within regions of the North Island of New Zealand that gave rise to the various levels of UMF® activity. Analyses of morphological characteristics, chemotaxonomic essential oil profiles, and population genetics of L. scoparium populations were conducted, and the conclusions that were drawn from each of these were very similar. Two major divisions were identified, each divided into two varieties. The northern division, which contained the core populations from Northland and Waikato, represented the previously described L. scoparium var. incanum and L. scoparium var. linifolium. This division yielded mānuka honey with high UMF® activity. The southern division, which contained the core populations from the Central North Island and East Coast, represented the previously described L. scoparium var. myrtifolium and an unnamed variety. The latter, growing principally on the East Coast, uniquely contains triketones essential oils. The southern division yielded mānuka honey with low UMF® activity. Hybridisation between these varieties will occur, leading to a continuum of UMF® activity in mānuka honey.

The data indicated multiple dispersions of L. scoparium to New Zealand from the evolutionary centre of the persistent-capsule Leptospermum group in south-east Australia, and later regional dispersal in New Zealand.

From this study two hypotheses were accepted: the variability in the UMF® activity of mānuka honey is due to both the dilution of mānuka honey by other honey types and the variety of L. scoparium harvested.

year: 2006

title: CoRes as tools for promoting pedagogical content knowledge  of novice science teachers

authors: Hume, Anne Christine

abstract: Expert science teachers possess a special blend of science content knowledge and pedagogical knowledge for teaching particular science topics to particular groups of students that is built up over time and experience. This form of professional knowledge, termed pedagogical content knowledge (PCK) by Shulman (1987), is topic-specific, unique to each science teacher, and can only be gained through teaching practice. The academic construct of PCK is a recognition that teaching is not simply the transmission of concepts and skills from teacher to students but, rather, a complex and problematic activity that requires many and varied ‘on the spot’ decisions and responses to students’ ongoing learning needs. Much has been written about the nature of PCK since Shulman first introduced the concept in 1987, and its elusive characteristics have led to much debate.

text: CoRes as tools for promoting pedagogical content knowledge  of novice science teachers Expert science teachers possess a special blend of science content knowledge and pedagogical knowledge for teaching particular science topics to particular groups of students that is built up over time and experience. This form of professional knowledge, termed pedagogical content knowledge (PCK) by Shulman (1987), is topic-specific, unique to each science teacher, and can only be gained through teaching practice. The academic construct of PCK is a recognition that teaching is not simply the transmission of concepts and skills from teacher to students but, rather, a complex and problematic activity that requires many and varied ‘on the spot’ decisions and responses to students’ ongoing learning needs. Much has been written about the nature of PCK since Shulman first introduced the concept in 1987, and its elusive characteristics have led to much debate.

year: 2010

title: Contrasting microbial communities across anthropogenic pollution gradients: MV Rena shipwreck versus urban pressures

authors: Reihana, Kiri

subjects: microbial

abstract: With the catastrophic environmental event that was the MV Rena ship wreck and oil spill in the Bay of Plenty area in 2011, a serendipitous opportunity to laterally explore a variety of impacts in the marine biosphere emerged. One of those opportunities is studied here; how microbial communities respond to stress in context of existing ambient local anthropogenic influences. The locational backdrop to this study is also relevant and provides an additional element of complexity as the Bay of Plenty region is located in the middle of the Taupo Volcanic Zone, hence microbial communities are likely to reflect the regional geology. Therefore a research project was possible where a pollutant gradient was selected based on a range of locations reflecting different elements of natural versus human long term and acute impact stresses. The concept was to establish a platform of preliminary study from which more detailed could follow. In addition this study aimed at providing a link between microbial environmental responses to stress in a context that could permit uptake of scientific evidence for cultural impact assessments given the strong cultural focus on MV Rena impacts by government and the public.
A significant element of the exploratory process was to identify methodologies which may be of use in ascribing microbial community composition with the microenvironment in a manner that allows comparison with research reported in the literature.
Review of all results showed that sites from widely varying geologies and environmental/stress condition were more similar than was initially predicted. This is in sharp contrast to expectations generated from the literature. 
At the sites of Astrolabe Reef (Otaiti) (impacted by the ship wreck, major oil spill and heavy metal pollution event) and Maketu (impacted by a eutrophic estuary), similar consortia transpired, despite strongly different sources of stress, substantiating the microbial biogeography theory: ‘Everything is everywhere’ and ‘the environment selects’. However at Tauranga Harbour long term human activity appears to have had a significant ‘additional’ effect on microbial communities. Our challenge has hence become one to separate out natural versus a range of anthropogenic stressors. 
Underlying the general overall trends, subtle community effects such as at Astrolabe Reef, which supported significantly different dominant communities to the other two sites. It was expected that Tauranga Harbour and Maketu Estuary (adjacent coastally), would be more similar. Of all the sites one at the impacted Maketu, site 1 (M1) and the impacted Tauranga site 2 (T2), were highly similar at 96% from statistical analysis suggesting, the original assumption of visually similar site characteristics (impacted sites with low residence time and thick algal matts) do support similar microbial communities. Substantiating the theory that ‘everything is everywhere’ at these two sites, even though the variables and elemental composition were dissimilar. 
Rare and abundant species were separated out and analysed to elucidate patterns or trends in the communities. Overall the abundant species clustered, into impacted versus non impacted sites, for the rare species Tauranga and Maketu clustered clearly following the same trend; while the Astrolabe reef sites clustered together. The clear differentiation in the rare species from the Astrolabe reef sites, suggests that those species may make up the endemic bacterial communities at Astrolabe Reef with additional effects of Rena pollution suggested at some of the sites close to the wreck.
A general discussion of the cultural and economic impacts of the Rena oil spill, gave context to the holistic human impacts sustained by the local Māori, specifically the disconnect of their precious livelihoods and unique relationship with the environment. These holistic concepts could compromise a thesis in themselves so in this report their definitions are by no means finite.     
This study attempted to interrelate both cultural, ecological biology and microbiology science components for the purpose of a more comprehensive investigation of microbial responses to the Rena oil spill and contrast these to local anthropogenic pressures. However being the first attempt to catalogue the previously unknown microbial communities in the Bay of Plenty area, meant that sample regimes, techniques, processing and analysis were all experimental. Despite this, effects attributable to the Rena were identified that lay beyond the large scale variability identified for Bay of Plenty microbial community assemblages. 
These research results provide an important primary contribution to microbial understandings in the New Zealand context, and as a baseline mapping exercise for the Bay of Plenty region.

text: Contrasting microbial communities across anthropogenic pollution gradients: MV Rena shipwreck versus urban pressures With the catastrophic environmental event that was the MV Rena ship wreck and oil spill in the Bay of Plenty area in 2011, a serendipitous opportunity to laterally explore a variety of impacts in the marine biosphere emerged. One of those opportunities is studied here; how microbial communities respond to stress in context of existing ambient local anthropogenic influences. The locational backdrop to this study is also relevant and provides an additional element of complexity as the Bay of Plenty region is located in the middle of the Taupo Volcanic Zone, hence microbial communities are likely to reflect the regional geology. Therefore a research project was possible where a pollutant gradient was selected based on a range of locations reflecting different elements of natural versus human long term and acute impact stresses. The concept was to establish a platform of preliminary study from which more detailed could follow. In addition this study aimed at providing a link between microbial environmental responses to stress in a context that could permit uptake of scientific evidence for cultural impact assessments given the strong cultural focus on MV Rena impacts by government and the public.
A significant element of the exploratory process was to identify methodologies which may be of use in ascribing microbial community composition with the microenvironment in a manner that allows comparison with research reported in the literature.
Review of all results showed that sites from widely varying geologies and environmental/stress condition were more similar than was initially predicted. This is in sharp contrast to expectations generated from the literature. 
At the sites of Astrolabe Reef (Otaiti) (impacted by the ship wreck, major oil spill and heavy metal pollution event) and Maketu (impacted by a eutrophic estuary), similar consortia transpired, despite strongly different sources of stress, substantiating the microbial biogeography theory: ‘Everything is everywhere’ and ‘the environment selects’. However at Tauranga Harbour long term human activity appears to have had a significant ‘additional’ effect on microbial communities. Our challenge has hence become one to separate out natural versus a range of anthropogenic stressors. 
Underlying the general overall trends, subtle community effects such as at Astrolabe Reef, which supported significantly different dominant communities to the other two sites. It was expected that Tauranga Harbour and Maketu Estuary (adjacent coastally), would be more similar. Of all the sites one at the impacted Maketu, site 1 (M1) and the impacted Tauranga site 2 (T2), were highly similar at 96% from statistical analysis suggesting, the original assumption of visually similar site characteristics (impacted sites with low residence time and thick algal matts) do support similar microbial communities. Substantiating the theory that ‘everything is everywhere’ at these two sites, even though the variables and elemental composition were dissimilar. 
Rare and abundant species were separated out and analysed to elucidate patterns or trends in the communities. Overall the abundant species clustered, into impacted versus non impacted sites, for the rare species Tauranga and Maketu clustered clearly following the same trend; while the Astrolabe reef sites clustered together. The clear differentiation in the rare species from the Astrolabe reef sites, suggests that those species may make up the endemic bacterial communities at Astrolabe Reef with additional effects of Rena pollution suggested at some of the sites close to the wreck.
A general discussion of the cultural and economic impacts of the Rena oil spill, gave context to the holistic human impacts sustained by the local Māori, specifically the disconnect of their precious livelihoods and unique relationship with the environment. These holistic concepts could compromise a thesis in themselves so in this report their definitions are by no means finite.     
This study attempted to interrelate both cultural, ecological biology and microbiology science components for the purpose of a more comprehensive investigation of microbial responses to the Rena oil spill and contrast these to local anthropogenic pressures. However being the first attempt to catalogue the previously unknown microbial communities in the Bay of Plenty area, meant that sample regimes, techniques, processing and analysis were all experimental. Despite this, effects attributable to the Rena were identified that lay beyond the large scale variability identified for Bay of Plenty microbial community assemblages. 
These research results provide an important primary contribution to microbial understandings in the New Zealand context, and as a baseline mapping exercise for the Bay of Plenty region.

year: 2016