title: Monitoring first year Maori students enrolled in selected Faculty of Arts and Social Sciences courses: A report prepared for the Dean of the Faculty of Arts and Social Sciences

authors: Levy, Michelle Patricia

subjects: maori students

abstract: Monitoring first year Maori Students Enrolled in Selected Faculty of Arts andSocial Sciences Courses. The total number of Maori students targeted by this project was 182, representing 93% of the total number of Maori students enrolled in Semester B level one courses. The majority of students participating in this initiative were first year students, although a small number of students taking 100 level courses were second, third or graduate year students. 11 Student views on the monitoring and support initiative Students were provided with the opportunity to comment on the monitoring and support initiative. All students contacted (49) recommended that this intervention continue for future first year Maori students enrolled in FASS.

text: Monitoring first year Maori students enrolled in selected Faculty of Arts and Social Sciences courses: A report prepared for the Dean of the Faculty of Arts and Social Sciences Monitoring first year Maori Students Enrolled in Selected Faculty of Arts andSocial Sciences Courses. The total number of Maori students targeted by this project was 182, representing 93% of the total number of Maori students enrolled in Semester B level one courses. The majority of students participating in this initiative were first year students, although a small number of students taking 100 level courses were second, third or graduate year students. 11 Student views on the monitoring and support initiative Students were provided with the opportunity to comment on the monitoring and support initiative. All students contacted (49) recommended that this intervention continue for future first year Maori students enrolled in FASS.

year: 2003-03-01

title: Research and development absorptive capacity: a Māori perspective

authors: Ruckstuhl, Katharina

subjects: computer science

abstract: This paper presents a view of research and development absorptive capacity from a Māori perspective. The assessment is part of a case study of a longitudinal programme – Science for Technological Innovation: Kia kotahi mai – Te Ao Putaiao me te Ao Hanagarau that aims to increase Aotearoa New Zealand’s capacity to use sci-tech for economic benefit. The paper finds that while Aotearoa New Zealand’s macro policy and meso institutional levels have become more responsive to Māori research and development demands, at the micro level of the individual or the firm there are still constraints given the small numbers of Māori in science, technology, engineering and mathematics. In response to this, a novel model of Māori sci-tech capacity is under development that considers not only research and development technical capacities, but also the human and relational capacities required to accelerate absorptive capacity to respond to Māori social and economic aspirations.

text: Research and development absorptive capacity: a Māori perspective This paper presents a view of research and development absorptive capacity from a Māori perspective. The assessment is part of a case study of a longitudinal programme – Science for Technological Innovation: Kia kotahi mai – Te Ao Putaiao me te Ao Hanagarau that aims to increase Aotearoa New Zealand’s capacity to use sci-tech for economic benefit. The paper finds that while Aotearoa New Zealand’s macro policy and meso institutional levels have become more responsive to Māori research and development demands, at the micro level of the individual or the firm there are still constraints given the small numbers of Māori in science, technology, engineering and mathematics. In response to this, a novel model of Māori sci-tech capacity is under development that considers not only research and development technical capacities, but also the human and relational capacities required to accelerate absorptive capacity to respond to Māori social and economic aspirations.

year: 2019

title: Investigating drivers of cyanobacterial blooms in Aotearoa – New Zealand lakes using sedimentary ancient DNA

authors: Picard, Maïlys

subjects: Cyanobacteria

abstract: Healthy lake ecosystems support biodiversity and human populations. They provide many ecosystem services such as water, food and energy. Lakes can be impacted by natural disturbances, but they are increasingly threatened by human-induced disturbances. Studies have shown that eutrophication and climate change often enhance cyanobacteria over other photosynthetic taxa. As cyanobacterial blooms are becoming more frequent and intense throughout the world, more lake systems are being investigated. In some cases there is not a clear link between eutrophication and cyanobacterial blooms. One such example is Lake Pounui (Wairarapa, New Zealand), which has little intensive agriculture in its catchment but water quality has degraded markedly in the last decade. The lake now experiences heavy cyanobacterial blooms every summer. This could be due to the presence of a non-native fish population, the European perch (Perca fluviatilis). This thesis examined the relationship between cyanobacterial blooms and perch introduction in New Zealand
lakes, including a multi-trophic study in Lake Pounui. Perch were introduced c. 1870 in New Zealand but introduction records are patchy and sometimes non-existent. Moreover, most lake systems are not studied until they are already degraded. This thesis used a combination of traditional proxies (pollen, charcoal, pigments) and modern proxies (sedimentary ancient DNA, XRF scanning) from lake sediment cores to reconstruct lake ecology in pre-human times, after M¯aori settlement between the 13th to 15th century, and after European settlement from 1840 AD. Timelines and intensity of human impact were reconstructed with pollen, charcoal analysis, and sediment dating when possible.

Cyanobacterial communities in six lakes were reconstructed through their sedimentary ancient DNA (sedaDNA) using metabarcoding and droplet digital PCR (ddPCR) in Chapter 2. Bloom-forming species were present in all lakes prior to human arrival; however their overall abundance was low. Total cyanobacteria abundance and richness increased in all lakes after European settlement but was very pronounced in four lakes, where bloom-forming taxa became dominant. The trends in cyanobacterial abundance from ddPCR were then compared to cyanobacterial pigments (canthaxanthin, echinenone, myxoxanthophyll and zeaxanthin) using highperformance liquid chromatography in Chapter 3, to assess the likelihood of the historical increase observed. Pigments / sedaDNA relationships were more consistent when all pigments were summed, which is likely due to differences in species composition across lakes. The positive correlations confirmed an increase in cyanobacterial biomass since European arrival.

Due to patchy records for fish introduction, fish sedimentary DNA was compared to environmental DNA (eDNA) from water samples as a methodological check (Chapter 4) before applying this method to the sediment cores. This study was undertaken in three small and shallow lowland lakes by targeting perch and rudd (Scardinius erythrophthalmus). Fish DNA was evenly distributed across the whole lake except when the fish population was low. Samples collected from the sediment contained fish DNA more often than water samples in two out of the three small shallow lakes (including Lake Pounui). Sediment geochemistry probably impeded detection in the third lake. Perch sedaDNA was therefore used as an indication of fish presence in Lake Pounui for Chapter 5, which explored multitrophic changes in Lake Pounui over the last c. 1,000 years. In addition to pollen, charcoal, and 14C dating, XRF scanning was used to reconstruct mineralogic shifts from the catchment (Ti/inc, K/inc) and within the lake (inc/coh). Biological trends were reconstructed
by targeting the sedaDNA of bacteria (16S rRNA), microeukaryotes (18S rRNA), metazoans (CO1), and macrophytes (rbcL, trnL). Complemented by historical records and studies, the data produced in this thesis indicated that the biggest changes in Lake Pounui happened after European settlement (c. 1845), with land clearance, perch introduction, climate change, and probable fertiliser application driving the degradation of the water quality in c. 180 years. This study revealed shifts in native communities (macrophytes, bacteria, oligochaete worms) and the appearance of new species (perch, macrophytes, freshwater nematodes) previously undocumented using sedaDNA. The results highlight just how complex yet fragile lake ecosystems can be and how little we still know about them. Sedimentary ancient DNA is a useful tool to study the insidious and long-lasting impact of nonnative species on freshwater ecosystems because it widens the range of species that can be studied. However, it needs to be complemented with other proxies. This thesis provides a framework to study fish DNA in small shallow lakes (Chapter 4). It can also inform future management and restoration strategies in lakes, especially in Lake Pounui, by retracing historical water quality (Chapter 2) and identifying taxa present prior to, during, and after lake degradation (Chapter 5).

text: Investigating drivers of cyanobacterial blooms in Aotearoa – New Zealand lakes using sedimentary ancient DNA Healthy lake ecosystems support biodiversity and human populations. They provide many ecosystem services such as water, food and energy. Lakes can be impacted by natural disturbances, but they are increasingly threatened by human-induced disturbances. Studies have shown that eutrophication and climate change often enhance cyanobacteria over other photosynthetic taxa. As cyanobacterial blooms are becoming more frequent and intense throughout the world, more lake systems are being investigated. In some cases there is not a clear link between eutrophication and cyanobacterial blooms. One such example is Lake Pounui (Wairarapa, New Zealand), which has little intensive agriculture in its catchment but water quality has degraded markedly in the last decade. The lake now experiences heavy cyanobacterial blooms every summer. This could be due to the presence of a non-native fish population, the European perch (Perca fluviatilis). This thesis examined the relationship between cyanobacterial blooms and perch introduction in New Zealand
lakes, including a multi-trophic study in Lake Pounui. Perch were introduced c. 1870 in New Zealand but introduction records are patchy and sometimes non-existent. Moreover, most lake systems are not studied until they are already degraded. This thesis used a combination of traditional proxies (pollen, charcoal, pigments) and modern proxies (sedimentary ancient DNA, XRF scanning) from lake sediment cores to reconstruct lake ecology in pre-human times, after M¯aori settlement between the 13th to 15th century, and after European settlement from 1840 AD. Timelines and intensity of human impact were reconstructed with pollen, charcoal analysis, and sediment dating when possible.

Cyanobacterial communities in six lakes were reconstructed through their sedimentary ancient DNA (sedaDNA) using metabarcoding and droplet digital PCR (ddPCR) in Chapter 2. Bloom-forming species were present in all lakes prior to human arrival; however their overall abundance was low. Total cyanobacteria abundance and richness increased in all lakes after European settlement but was very pronounced in four lakes, where bloom-forming taxa became dominant. The trends in cyanobacterial abundance from ddPCR were then compared to cyanobacterial pigments (canthaxanthin, echinenone, myxoxanthophyll and zeaxanthin) using highperformance liquid chromatography in Chapter 3, to assess the likelihood of the historical increase observed. Pigments / sedaDNA relationships were more consistent when all pigments were summed, which is likely due to differences in species composition across lakes. The positive correlations confirmed an increase in cyanobacterial biomass since European arrival.

Due to patchy records for fish introduction, fish sedimentary DNA was compared to environmental DNA (eDNA) from water samples as a methodological check (Chapter 4) before applying this method to the sediment cores. This study was undertaken in three small and shallow lowland lakes by targeting perch and rudd (Scardinius erythrophthalmus). Fish DNA was evenly distributed across the whole lake except when the fish population was low. Samples collected from the sediment contained fish DNA more often than water samples in two out of the three small shallow lakes (including Lake Pounui). Sediment geochemistry probably impeded detection in the third lake. Perch sedaDNA was therefore used as an indication of fish presence in Lake Pounui for Chapter 5, which explored multitrophic changes in Lake Pounui over the last c. 1,000 years. In addition to pollen, charcoal, and 14C dating, XRF scanning was used to reconstruct mineralogic shifts from the catchment (Ti/inc, K/inc) and within the lake (inc/coh). Biological trends were reconstructed
by targeting the sedaDNA of bacteria (16S rRNA), microeukaryotes (18S rRNA), metazoans (CO1), and macrophytes (rbcL, trnL). Complemented by historical records and studies, the data produced in this thesis indicated that the biggest changes in Lake Pounui happened after European settlement (c. 1845), with land clearance, perch introduction, climate change, and probable fertiliser application driving the degradation of the water quality in c. 180 years. This study revealed shifts in native communities (macrophytes, bacteria, oligochaete worms) and the appearance of new species (perch, macrophytes, freshwater nematodes) previously undocumented using sedaDNA. The results highlight just how complex yet fragile lake ecosystems can be and how little we still know about them. Sedimentary ancient DNA is a useful tool to study the insidious and long-lasting impact of nonnative species on freshwater ecosystems because it widens the range of species that can be studied. However, it needs to be complemented with other proxies. This thesis provides a framework to study fish DNA in small shallow lakes (Chapter 4). It can also inform future management and restoration strategies in lakes, especially in Lake Pounui, by retracing historical water quality (Chapter 2) and identifying taxa present prior to, during, and after lake degradation (Chapter 5).

year: 2023