title: Further characterization of glycine-containing microcystins from the McMurdo Dry Valleys of Antarctica

authors: Puddick, Jonathan

subjects: amino acid analysis

abstract: Microcystins are hepatotoxic cyclic peptides produced by several cyanobacterial genera worldwide. In 2008, our research group identified eight new glycine-containing microcystin congeners in two hydro-terrestrial mat samples from the McMurdo Dry Valleys of Eastern Antarctica. During the present study, high-resolution mass spectrometry, amino acid analysis and micro-scale thiol derivatization were used to further elucidate their structures. The Antarctic microcystin congeners contained the rare substitution of the position-1 D-alanine for glycine, as well as the acetyl desmethyl modification of the position-5 Adda moiety (3S-amino-9S-methoxy-2S,6,8S-trimethyl-10-phenyldeca-4E,6E-dienoic acid). Amino acid analysis was used to determine the stereochemistry of several of the amino acids and conclusively demonstrated the presence of glycine in the microcystins. A recently developed thiol derivatization technique showed that each microcystin contained dehydrobutyrine in position-7 instead of the commonly observed N-methyl dehydroalanine.

text: Further characterization of glycine-containing microcystins from the McMurdo Dry Valleys of Antarctica Microcystins are hepatotoxic cyclic peptides produced by several cyanobacterial genera worldwide. In 2008, our research group identified eight new glycine-containing microcystin congeners in two hydro-terrestrial mat samples from the McMurdo Dry Valleys of Eastern Antarctica. During the present study, high-resolution mass spectrometry, amino acid analysis and micro-scale thiol derivatization were used to further elucidate their structures. The Antarctic microcystin congeners contained the rare substitution of the position-1 D-alanine for glycine, as well as the acetyl desmethyl modification of the position-5 Adda moiety (3S-amino-9S-methoxy-2S,6,8S-trimethyl-10-phenyldeca-4E,6E-dienoic acid). Amino acid analysis was used to determine the stereochemistry of several of the amino acids and conclusively demonstrated the presence of glycine in the microcystins. A recently developed thiol derivatization technique showed that each microcystin contained dehydrobutyrine in position-7 instead of the commonly observed N-methyl dehydroalanine.

year: 2015-02-10

title: New Zealand trends in animal behaviour research

authors: Guadarrama-Maillot, Veronica

subjects: animal behaviour literature

abstract: To identify trends in research on pure ethology (fundamental issues in animal behaviour) in New Zealand, we combined manual and database searches of the five ethological journals with the greatest impact factors (Animal Behaviour, Behavioral Ecology, Behavioral Ecology and Sociobiology, Ethology and Behaviour) published from 1937 to (and including) 2007. We counted all articles giving a New Zealand address for the first-listed and/or reprint author. Our analyses showed (1) a total of 131 articles in the five journals, with Animal Behaviour, the journal of preference, containing nearly half of the articles collected; (2) a sharp and steady increase in articles published per year; (3) all four of Tinbergen’s questions received attention, with mechanistic and functional studies being more common than studies of development and evolutionary history; (4) a recent resurgence of mechanistic research, representing 67% of all studies in the period from 2003–07; (5) a balance in research focusing on the proximate and ultimate causes of behaviour, shifting towards proximate research in the early 1990s; (6) many New Zealand institutions published papers in the five journals, although universities were the main contributors; (7) only a small subset of the research (28.8%) was done with overseas collaboration; (8) a total of 139 authors were involved in the research reviewed (when all authors for each paper were considered without taking address into account); (9) a maturation of the sampled research by the early 1990s (i.e., when "descriptive" and "explanatory" papers were supplemented with "synthesis" papers); and (10) a large number of investigations (55%) used birds as the study subject. Ethology in New Zealand, as represented in the five leading animal behaviour journals, was found to be a diverse, dynamic and growing field.

text: New Zealand trends in animal behaviour research To identify trends in research on pure ethology (fundamental issues in animal behaviour) in New Zealand, we combined manual and database searches of the five ethological journals with the greatest impact factors (Animal Behaviour, Behavioral Ecology, Behavioral Ecology and Sociobiology, Ethology and Behaviour) published from 1937 to (and including) 2007. We counted all articles giving a New Zealand address for the first-listed and/or reprint author. Our analyses showed (1) a total of 131 articles in the five journals, with Animal Behaviour, the journal of preference, containing nearly half of the articles collected; (2) a sharp and steady increase in articles published per year; (3) all four of Tinbergen’s questions received attention, with mechanistic and functional studies being more common than studies of development and evolutionary history; (4) a recent resurgence of mechanistic research, representing 67% of all studies in the period from 2003–07; (5) a balance in research focusing on the proximate and ultimate causes of behaviour, shifting towards proximate research in the early 1990s; (6) many New Zealand institutions published papers in the five journals, although universities were the main contributors; (7) only a small subset of the research (28.8%) was done with overseas collaboration; (8) a total of 139 authors were involved in the research reviewed (when all authors for each paper were considered without taking address into account); (9) a maturation of the sampled research by the early 1990s (i.e., when "descriptive" and "explanatory" papers were supplemented with "synthesis" papers); and (10) a large number of investigations (55%) used birds as the study subject. Ethology in New Zealand, as represented in the five leading animal behaviour journals, was found to be a diverse, dynamic and growing field.

year: 2008

title: The 2020 cannabis referendum: Māori voter support, racialized policing, and the Criminal Justice System

authors: Dempster, Craig

abstract: In the New Zealand 2020 cannabis referendum, 50.7% of all voters rejected the creation of a legally-regulated cannabis market and instead supported retaining the current prohibitionist policy. Although the referendum failed to pass, a majority of Māori voted in favor of cannabis law reform. This paper suggests that within the Māori community there is a more nuanced appreciation of the impact of policing cannabis. Māori perceive that greater harm is caused by the racialized policing of cannabis than by the usage of it. Following McCreanor, et al. (2014), this paper employs a thematic, content analysis of the New Zealand Herald’s coverage of the 2020 cannabis referendum to investigate the presence of race-based targeting/policing in discussions of the legislation. The results reveal that racial disparities emerged as secondary to framing both the impact of cannabis and the referendum as race-neutral and affecting everyone in society equally. This paper argues that the impact of the policing of this particular drug impacts Māori differently, wherein they bear the brunt of racialized policing. Thus, Māori possess a more sophisticated understanding that warrants consideration because it is inextricably linked to lived experiences of policing that differ from wider social narratives of policing and drug policy in New Zealand.

text: The 2020 cannabis referendum: Māori voter support, racialized policing, and the Criminal Justice System In the New Zealand 2020 cannabis referendum, 50.7% of all voters rejected the creation of a legally-regulated cannabis market and instead supported retaining the current prohibitionist policy. Although the referendum failed to pass, a majority of Māori voted in favor of cannabis law reform. This paper suggests that within the Māori community there is a more nuanced appreciation of the impact of policing cannabis. Māori perceive that greater harm is caused by the racialized policing of cannabis than by the usage of it. Following McCreanor, et al. (2014), this paper employs a thematic, content analysis of the New Zealand Herald’s coverage of the 2020 cannabis referendum to investigate the presence of race-based targeting/policing in discussions of the legislation. The results reveal that racial disparities emerged as secondary to framing both the impact of cannabis and the referendum as race-neutral and affecting everyone in society equally. This paper argues that the impact of the policing of this particular drug impacts Māori differently, wherein they bear the brunt of racialized policing. Thus, Māori possess a more sophisticated understanding that warrants consideration because it is inextricably linked to lived experiences of policing that differ from wider social narratives of policing and drug policy in New Zealand.

year: 2022