title: Context counts : leading educational reform in New Zealand secondary schools

authors: Morrison, Michele

subjects: context

abstract: Educational leadership and organisational literature is replete with syntheses of effective leader behaviours, change sequences and reform models, yet few studies explore the nuanced manner in which individual leaders make sense of and respond to the dynamic influences shaping their thinking and practice over time. In posing the question, how do principals experience leadership during periods of intentional educational change, this study gains insight into the lived experience of five New Zealand secondary school principals as they lead school wide implementation of Phase 5 Te Kotahitanga, a reform initiative designed to reduce disparities in the educational achievement of indigenous Māori students through the introduction of a culturally responsive pedagogy of relations. The case narratives arising from a three year dialogue with participants examine the genesis of each principal’s commitment to equity in educational access, opportunity and outcome; their evolving thinking and practice; and the manner in which people and structures within each school field enable and constrain their leadership endeavours. Findings demonstrate that leading counter-hegemonic change is a situated, complex and demanding undertaking, the intensity of which eludes much of the leadership and change literature. Hermeneutic reading of these rich qualitative accounts of lived experience suggests that, far from being detached observers, leaders both embody and are embedded in context. This exposes the shortcomings of literature that dichotomises the two and makes Bourdieu’s lens on practice as a temporal, embodied, discursive and social activity an important heuristic through which to view the holism of change leadership. The implications arising from this study are fourfold: leadership narratives open windows to natural and vicarious meaning making, personal biography is of central rather than peripheral importance, emotional and micropolitical literacy crucial, and professional mentoring a potentially powerful catalyst in building practice wisdom.

text: Context counts : leading educational reform in New Zealand secondary schools Educational leadership and organisational literature is replete with syntheses of effective leader behaviours, change sequences and reform models, yet few studies explore the nuanced manner in which individual leaders make sense of and respond to the dynamic influences shaping their thinking and practice over time. In posing the question, how do principals experience leadership during periods of intentional educational change, this study gains insight into the lived experience of five New Zealand secondary school principals as they lead school wide implementation of Phase 5 Te Kotahitanga, a reform initiative designed to reduce disparities in the educational achievement of indigenous Māori students through the introduction of a culturally responsive pedagogy of relations. The case narratives arising from a three year dialogue with participants examine the genesis of each principal’s commitment to equity in educational access, opportunity and outcome; their evolving thinking and practice; and the manner in which people and structures within each school field enable and constrain their leadership endeavours. Findings demonstrate that leading counter-hegemonic change is a situated, complex and demanding undertaking, the intensity of which eludes much of the leadership and change literature. Hermeneutic reading of these rich qualitative accounts of lived experience suggests that, far from being detached observers, leaders both embody and are embedded in context. This exposes the shortcomings of literature that dichotomises the two and makes Bourdieu’s lens on practice as a temporal, embodied, discursive and social activity an important heuristic through which to view the holism of change leadership. The implications arising from this study are fourfold: leadership narratives open windows to natural and vicarious meaning making, personal biography is of central rather than peripheral importance, emotional and micropolitical literacy crucial, and professional mentoring a potentially powerful catalyst in building practice wisdom.

year: 2019

title: Researching the Process of Auahi Kore for Marae

authors: Masters-Awatere, Bridgette

abstract: • The current report is piece of a qualitative research exploring issues relating the development of Marae Auahi Kore. Such aspects as; factors that aid or impede the process, resources available or required by marae, and people's perceptions of the Auahi Kore brand were explored.

• Interviews were conducted with people from a variety of backgrounds. Research participants included: Marae Trustees, Public Health Workers, Runanga Staff as well as Kaumatua and Kuia. These people were identified as key informants for the current project. A total of 25 people were interviewed during March and April using an open interview schedule.

• Exploration into the Smokefree policies of marae was not pursued within the current project as this was not the focus nor the intent of the research. Project constraints such as time and budget meant there was a limit to what could be undertaken for the current piece of research.

text: Researching the Process of Auahi Kore for Marae • The current report is piece of a qualitative research exploring issues relating the development of Marae Auahi Kore. Such aspects as; factors that aid or impede the process, resources available or required by marae, and people's perceptions of the Auahi Kore brand were explored.

• Interviews were conducted with people from a variety of backgrounds. Research participants included: Marae Trustees, Public Health Workers, Runanga Staff as well as Kaumatua and Kuia. These people were identified as key informants for the current project. A total of 25 people were interviewed during March and April using an open interview schedule.

• Exploration into the Smokefree policies of marae was not pursued within the current project as this was not the focus nor the intent of the research. Project constraints such as time and budget meant there was a limit to what could be undertaken for the current piece of research.

year: 2000

title: Identifying the signalling pathway of a novel  Myostatin Splice Variant (MSV)

authors: Hennebry, Alex

subjects: myostatin

abstract: Myostatin (Mstn), a member of the transforming growth factor-β super family, is a potent negative regulator of skeletal muscle mass. Studies delineating the function of Mstn have identified multiple signal transduction pathways that convey the Mstn signal. Mstn has been shown to influence canonical TGF-β, mitogen activated protein kinase (MAPK) and the PI3K/AKT signal transduction cascades. The discovery in our laboratory of a novel splice variant of Mstn (MSV) that opposes Mstn and stimulates the proliferation of myoblasts provided the impetus for the investigations in this thesis. The splicing of MSV was restricted to the Cetartiodactyl clade of mammals, and MSV may represent an intragenic regulator of Mstn. Thus, the studies undertaken in this thesis were to delineate the signalling pathways used by Mstn and MSV in order to understand how their opposing roles in myoblasts regulate myogenesis.
Initially, microarray analysis was used to investigate the transcriptional responses of ovine myoblasts following exposure to recombinant Mstn (eukaryotic) and MSV (prokaryotic) protein. Mstn treatment induced changes in number of transcripts, with changes consistent with previous investigations, for example increased interlukin-6 (IL6) and decreased MyoD. In addition, a novel transcriptional target of Mstn, the β1 subunit of the Na⁺-K⁺-ATPase was discovered. Treatment of ovine myoblasts with recombinant MSV induced a plethora of transcriptional responses. IPA analysis suggested a number of these were due to LPS (endotoxin) contamination, which could be attributed to the production of this protein in E. coli. This was confirmed using the Limulus amebocyte lysate assay. Phase separation using Triton-X 114 proved an effective method for the removal of LPS from the MSV preparation.
Western blot analysis was performed following the treatment of ovine myoblasts with Mstn and purified MSV. Consistent with previous myoblast studies Mstn stimulated canonical TGF-β (Smad) signalling and the p38 and ERK components of the MAPK signalling cascade. In contrast to previous studies, Mstn also stimulated AKT signalling, with specific phosphorylation of serine 473 (AKTS⁴⁷³). In addition, Mstn altered the abundance of multiple myogenic transcription factors (MyoD, Myf5, MRF4, Pax7 and Mef2) and the abundance and/or the phosphorylation of targets that have a metabolic role in skeletal muscle (rps6, 4EBP1 and p70S6K). Treatment with MSV increased the abundance of Smad 3, Myf5, 4EBP1 and stimulated AKTS⁴⁷³and 4EBP1 phosphorylation. These data provided the foundation for confirmation of these pathways targeted by MSV in C₂C₁₂ myoblasts that stably expressed MSV.
C₂C₁₂ cells expressing MSV had an increased proliferative capacity and showed increased mitochondrial activity (EZ4U assay) as compared to controls. These cells showed an increased abundance of the MRFs MyoD, MRF4 and Myogenin and an increased abundance or phosphorylation of signalling targets involved in canonical TGF-β, MAPK and PI3K/AKT signalling cascades. In addition, cells expressing MSV had an increased abundance and phosphorylation of acetyl co-enzyme A carboxylase (ACC) and 4EBP1, which have established roles in regulating metabolism and the synthesis of protein.
The significant overlap of processes influenced by the Na⁺ , K⁺ ATPase complex and Mstn prompted an investigation on how Mstn regulates the β1 subunit of the Na⁺ , K⁺ ATPase. This transcriptional response was found to be dependent on the Smad pathway. In addition, these studies also show that Na⁺ K⁺ ATPase activity plays a role in proliferation and differentiation of ovine myoblasts and suggest that Mstn inhibits ion flow controlled through the function of this enzyme complex. 
In conclusion, these studies show that Mstn and MSV share a number of common signalling targets. In contrast to previous studies of Mstn, the stable expression MSV increases the activation of AKT signalling and increases the abundance of key myogenic transcription factors. In addition, MSV increases the abundance and phosphorylation of ACC and 4EBP1, molecules involved regulating the synthesis of protein and fatty acids. In addition, the β1 subunit of the Na⁺ K⁺ ATPase, was identified as a novel transcriptional target of Mstn, with this regulation controlled through a Smad dependant mechanism. These data confirm the postulate that Mstn and MSV have divergent signalling functions and suggest a role for MSV in the control of oxidative metabolism.

text: Identifying the signalling pathway of a novel  Myostatin Splice Variant (MSV) Myostatin (Mstn), a member of the transforming growth factor-β super family, is a potent negative regulator of skeletal muscle mass. Studies delineating the function of Mstn have identified multiple signal transduction pathways that convey the Mstn signal. Mstn has been shown to influence canonical TGF-β, mitogen activated protein kinase (MAPK) and the PI3K/AKT signal transduction cascades. The discovery in our laboratory of a novel splice variant of Mstn (MSV) that opposes Mstn and stimulates the proliferation of myoblasts provided the impetus for the investigations in this thesis. The splicing of MSV was restricted to the Cetartiodactyl clade of mammals, and MSV may represent an intragenic regulator of Mstn. Thus, the studies undertaken in this thesis were to delineate the signalling pathways used by Mstn and MSV in order to understand how their opposing roles in myoblasts regulate myogenesis.
Initially, microarray analysis was used to investigate the transcriptional responses of ovine myoblasts following exposure to recombinant Mstn (eukaryotic) and MSV (prokaryotic) protein. Mstn treatment induced changes in number of transcripts, with changes consistent with previous investigations, for example increased interlukin-6 (IL6) and decreased MyoD. In addition, a novel transcriptional target of Mstn, the β1 subunit of the Na⁺-K⁺-ATPase was discovered. Treatment of ovine myoblasts with recombinant MSV induced a plethora of transcriptional responses. IPA analysis suggested a number of these were due to LPS (endotoxin) contamination, which could be attributed to the production of this protein in E. coli. This was confirmed using the Limulus amebocyte lysate assay. Phase separation using Triton-X 114 proved an effective method for the removal of LPS from the MSV preparation.
Western blot analysis was performed following the treatment of ovine myoblasts with Mstn and purified MSV. Consistent with previous myoblast studies Mstn stimulated canonical TGF-β (Smad) signalling and the p38 and ERK components of the MAPK signalling cascade. In contrast to previous studies, Mstn also stimulated AKT signalling, with specific phosphorylation of serine 473 (AKTS⁴⁷³). In addition, Mstn altered the abundance of multiple myogenic transcription factors (MyoD, Myf5, MRF4, Pax7 and Mef2) and the abundance and/or the phosphorylation of targets that have a metabolic role in skeletal muscle (rps6, 4EBP1 and p70S6K). Treatment with MSV increased the abundance of Smad 3, Myf5, 4EBP1 and stimulated AKTS⁴⁷³and 4EBP1 phosphorylation. These data provided the foundation for confirmation of these pathways targeted by MSV in C₂C₁₂ myoblasts that stably expressed MSV.
C₂C₁₂ cells expressing MSV had an increased proliferative capacity and showed increased mitochondrial activity (EZ4U assay) as compared to controls. These cells showed an increased abundance of the MRFs MyoD, MRF4 and Myogenin and an increased abundance or phosphorylation of signalling targets involved in canonical TGF-β, MAPK and PI3K/AKT signalling cascades. In addition, cells expressing MSV had an increased abundance and phosphorylation of acetyl co-enzyme A carboxylase (ACC) and 4EBP1, which have established roles in regulating metabolism and the synthesis of protein.
The significant overlap of processes influenced by the Na⁺ , K⁺ ATPase complex and Mstn prompted an investigation on how Mstn regulates the β1 subunit of the Na⁺ , K⁺ ATPase. This transcriptional response was found to be dependent on the Smad pathway. In addition, these studies also show that Na⁺ K⁺ ATPase activity plays a role in proliferation and differentiation of ovine myoblasts and suggest that Mstn inhibits ion flow controlled through the function of this enzyme complex. 
In conclusion, these studies show that Mstn and MSV share a number of common signalling targets. In contrast to previous studies of Mstn, the stable expression MSV increases the activation of AKT signalling and increases the abundance of key myogenic transcription factors. In addition, MSV increases the abundance and phosphorylation of ACC and 4EBP1, molecules involved regulating the synthesis of protein and fatty acids. In addition, the β1 subunit of the Na⁺ K⁺ ATPase, was identified as a novel transcriptional target of Mstn, with this regulation controlled through a Smad dependant mechanism. These data confirm the postulate that Mstn and MSV have divergent signalling functions and suggest a role for MSV in the control of oxidative metabolism.

year: 2014