title: An interactive graphic for visually fitting the generalized extreme value distribution

authors: Paramonov, Viacheslav

subjects: visual fitting

abstract: A utility developed for the Windows operating system enables easy visual curve fitting of the generalized extreme value distribution (GEV), from a screen display of annual maxima in a Gumbel plot. This gives a useful indication of how positioning of curves passing through the data points may result in similar or different return periods for large events. The utility employs a general risk-based time axis, with the usual return period being a special case option.

text: An interactive graphic for visually fitting the generalized extreme value distribution A utility developed for the Windows operating system enables easy visual curve fitting of the generalized extreme value distribution (GEV), from a screen display of annual maxima in a Gumbel plot. This gives a useful indication of how positioning of curves passing through the data points may result in similar or different return periods for large events. The utility employs a general risk-based time axis, with the usual return period being a special case option.

year: 2020

title: RNAi-mediated Reduction of a Major Ruminant Specific Milk Allergen Using a Transgenic Mouse Model

authors: Jabed, Md Anower

subjects: BLG, miRNA, RNAi, Transgenic mouse, Gene knockdown

abstract: Milk from dairy cows is an important human food. It contains the protein beta-lactoglobulin (BLG) which is not present in human milk and thought to be a major allergen in the milk of cows. RNA interference (RNAi), mediated by sequence-specific micro RNAs (miRNAs), provides a new molecular tool to reduce the levels of undesirable proteins. 

In this study, I have screened ten miRNAs targeting the BLG mRNA for their potential to knockdown BLG expression using a cell-based in vitro assay. I identified four miRNAs which showed substantial bovine BLG (bBLG) (78-97%) and ovine BLG (oBLG) (33-94%) knockdown activity in vitro. Tandem miRNA constructs with combinations of these four single miRNAs did not result in significantly increased knockdown efficiency compared to the respective single miRNA constructs in the in vitro assay (P>0.05). Because targeting of two different regions has the potential for greater knockdown efficiency in vivo, I chose a tandem construct which achieved in vitro up to 99% bBLG knockdown as compared to 92% and 74% of bBLG knockdown by the single miRNAs. The tandem construct also showed 90% of oBLG knockdown in comparison to 92% and 69% of oBLG knockdown by the single miRNAs in the in vitro assay which made it an ideal candidate for the subsequent evaluation in transgenic (Tg) mice. For the in vivo studies, the cytomegalovirus (CMV) promoter of the selected miRNA constructs was replaced with the promoter of the mouse milk protein gene for whey acid protein (WAP) which directs the expression of the BLG-specific miRNAs to the lactating mammary gland. The Tg mouse lines generated with the tissue-specific tandem construct and the single miRNAs used for the tandem miRNA construct were then crossed with an oBLG or bBLG expressing Tg mouse line to generate double transgenic (miRNA-BLG) mice for assessing BLG knockdown in vivo. Analysis of the milk proteins demonstrated that the tandem miRNA reduced the levels of oBLG and bBLG in milk from the miRNA-BLG mice up to 96% and 46%, respectively. This study validates the mammary gland specific expression of miRNAs as a promising approach to knockdown allergenic proteins in milk.

text: RNAi-mediated Reduction of a Major Ruminant Specific Milk Allergen Using a Transgenic Mouse Model Milk from dairy cows is an important human food. It contains the protein beta-lactoglobulin (BLG) which is not present in human milk and thought to be a major allergen in the milk of cows. RNA interference (RNAi), mediated by sequence-specific micro RNAs (miRNAs), provides a new molecular tool to reduce the levels of undesirable proteins. 

In this study, I have screened ten miRNAs targeting the BLG mRNA for their potential to knockdown BLG expression using a cell-based in vitro assay. I identified four miRNAs which showed substantial bovine BLG (bBLG) (78-97%) and ovine BLG (oBLG) (33-94%) knockdown activity in vitro. Tandem miRNA constructs with combinations of these four single miRNAs did not result in significantly increased knockdown efficiency compared to the respective single miRNA constructs in the in vitro assay (P>0.05). Because targeting of two different regions has the potential for greater knockdown efficiency in vivo, I chose a tandem construct which achieved in vitro up to 99% bBLG knockdown as compared to 92% and 74% of bBLG knockdown by the single miRNAs. The tandem construct also showed 90% of oBLG knockdown in comparison to 92% and 69% of oBLG knockdown by the single miRNAs in the in vitro assay which made it an ideal candidate for the subsequent evaluation in transgenic (Tg) mice. For the in vivo studies, the cytomegalovirus (CMV) promoter of the selected miRNA constructs was replaced with the promoter of the mouse milk protein gene for whey acid protein (WAP) which directs the expression of the BLG-specific miRNAs to the lactating mammary gland. The Tg mouse lines generated with the tissue-specific tandem construct and the single miRNAs used for the tandem miRNA construct were then crossed with an oBLG or bBLG expressing Tg mouse line to generate double transgenic (miRNA-BLG) mice for assessing BLG knockdown in vivo. Analysis of the milk proteins demonstrated that the tandem miRNA reduced the levels of oBLG and bBLG in milk from the miRNA-BLG mice up to 96% and 46%, respectively. This study validates the mammary gland specific expression of miRNAs as a promising approach to knockdown allergenic proteins in milk.

year: 2012

title: Possibilities of transformation: discourses of difference in organisational communication

authors: Jones, Deborah Helen

abstract: In this thesis I set out to create an interdisciplinary location from which to address the relationship between gender and communication in an organisational context. I draw on feminist and post-structuralist theory to ask the question: How is ‘difference’ constructed in organisational communication? I use the term ‘difference’ here to indicate that I am interested in how ‘gender’ works in relationship with other kinds of difference, especially in relation to ethnic difference.

My thesis is intended as a contribution to feminist communication theory, and specifically, to the emerging field of feminist organisational communication. Because of the approach I take to ‘communication’, this project addresses broad issues of identity, agency and discourse in organisations. In this sense, I also see the thesis as a contribution to organisational studies, and particularly to the study of organisational discourse, which opens up new relationships between ‘organisation’ and ‘communication’. Interdisciplinarity itself is a key issue in my thesis, as I set out to create connections between disciplinary fields in the service of contesting them, rather than seeking to create new boundaries. A second key focus is the development of theoretical sophistication in the field of gender and communication. I draw on feminist/post-structuralist theory, especially feminist readings of Michel Foucault’s work to generate this development. My third key focus is the issue of agency, which I see as central to theorising organisational transformation, and also as central to re-thinking communication theory.

My writing strategies demonstrate my commitments to reflexivity in developing feminist/post-structuralist research epistemologies, and I experiment with ways of paying attention to issues of authority throughout the thesis. This thesis is divided into two main parts. In Chapters 2 to 6, I set up a theoretical framework for feminist/post-structuralist accounts of gender and ethnicity, and weave this theory through the literatures of ‘gender and communication’, ‘cross-cultural communication’, and ‘organisational communication’. I set out to produce an ‘autocritique’, an opportunity to draw on developments in post-structuralist theory to think differently about feminist ‘organisational communication’, putting it within a broader framework of ‘difference’.

In the second part of the thesis, Chapters 7 to 12, I put issues generated by this theoretical framework in a specific organisational context, asking: How are gender and ethnic differences constructed in the discourses of Equal Employment Opportunities (EEO) and Biculturalism in New Zealand government organisations? Chapters 7 and 8 introduce my field studies methodology and research subjects, and provide background narratives which frame the historical and cultural context in which they were carried out. In Chapters 9 to 12 I carry out a discourse analysis of interviews with EEO and Biculturalism practitioners, and also analyse published and unpublished documents associated with ‘difference’ in employment policies. These accounts of field studies are intended as a series of experiments with theorising ‘organisational communication’ in different ways. I complete the thesis with a ‘Review’ (Chapter 13) in which I reflect on key theoretical threads, and on further questions that emerged during the project.

text: Possibilities of transformation: discourses of difference in organisational communication In this thesis I set out to create an interdisciplinary location from which to address the relationship between gender and communication in an organisational context. I draw on feminist and post-structuralist theory to ask the question: How is ‘difference’ constructed in organisational communication? I use the term ‘difference’ here to indicate that I am interested in how ‘gender’ works in relationship with other kinds of difference, especially in relation to ethnic difference.

My thesis is intended as a contribution to feminist communication theory, and specifically, to the emerging field of feminist organisational communication. Because of the approach I take to ‘communication’, this project addresses broad issues of identity, agency and discourse in organisations. In this sense, I also see the thesis as a contribution to organisational studies, and particularly to the study of organisational discourse, which opens up new relationships between ‘organisation’ and ‘communication’. Interdisciplinarity itself is a key issue in my thesis, as I set out to create connections between disciplinary fields in the service of contesting them, rather than seeking to create new boundaries. A second key focus is the development of theoretical sophistication in the field of gender and communication. I draw on feminist/post-structuralist theory, especially feminist readings of Michel Foucault’s work to generate this development. My third key focus is the issue of agency, which I see as central to theorising organisational transformation, and also as central to re-thinking communication theory.

My writing strategies demonstrate my commitments to reflexivity in developing feminist/post-structuralist research epistemologies, and I experiment with ways of paying attention to issues of authority throughout the thesis. This thesis is divided into two main parts. In Chapters 2 to 6, I set up a theoretical framework for feminist/post-structuralist accounts of gender and ethnicity, and weave this theory through the literatures of ‘gender and communication’, ‘cross-cultural communication’, and ‘organisational communication’. I set out to produce an ‘autocritique’, an opportunity to draw on developments in post-structuralist theory to think differently about feminist ‘organisational communication’, putting it within a broader framework of ‘difference’.

In the second part of the thesis, Chapters 7 to 12, I put issues generated by this theoretical framework in a specific organisational context, asking: How are gender and ethnic differences constructed in the discourses of Equal Employment Opportunities (EEO) and Biculturalism in New Zealand government organisations? Chapters 7 and 8 introduce my field studies methodology and research subjects, and provide background narratives which frame the historical and cultural context in which they were carried out. In Chapters 9 to 12 I carry out a discourse analysis of interviews with EEO and Biculturalism practitioners, and also analyse published and unpublished documents associated with ‘difference’ in employment policies. These accounts of field studies are intended as a series of experiments with theorising ‘organisational communication’ in different ways. I complete the thesis with a ‘Review’ (Chapter 13) in which I reflect on key theoretical threads, and on further questions that emerged during the project.

year: 1998