title: Lock-in thermography using miniature infra-red cameras and integrated actuators for defect identification in composite materials

authors: Ólafsson, G.

abstract: A novel approach for thermographic Non-Destructive Evaluation (NDE) of laminated polymer composite structures is presented. The technique is based on Lock-In Thermography (LIT), which traditionally uses an external heat source. Here a new means of internal heating, via a lightweight embedded actuator capable of providing highly repeatable and uniform heating is presented. The equipment cost, complexity and hence size is reduced by removing the need for high power external thermal excitation. Instead, the necessary temperature modulation of the internal actuator is achieved with a compact and low-cost Arduino controlled circuit. The size and cost of the equipment is further reduced by demonstrating that a miniature printed circuit board mounted thermal core type micro-bolometer can be used effectively for LIT. The performance of the thermal core is quantitatively compared with the more expensive and bulky traditional infra-red cameras. It is shown that the thermal core can detect defects with similar overall performance as a cooled photon detector and an uncooled micro-bolometer. The low-cost, compact nature and small mass of the thermal core offers great potential in thermographic inspection opening the possibility of deploying devices permanently on structures in conjunction with the embedded actuators for in-situ monitoring in the service environment.

text: Lock-in thermography using miniature infra-red cameras and integrated actuators for defect identification in composite materials A novel approach for thermographic Non-Destructive Evaluation (NDE) of laminated polymer composite structures is presented. The technique is based on Lock-In Thermography (LIT), which traditionally uses an external heat source. Here a new means of internal heating, via a lightweight embedded actuator capable of providing highly repeatable and uniform heating is presented. The equipment cost, complexity and hence size is reduced by removing the need for high power external thermal excitation. Instead, the necessary temperature modulation of the internal actuator is achieved with a compact and low-cost Arduino controlled circuit. The size and cost of the equipment is further reduced by demonstrating that a miniature printed circuit board mounted thermal core type micro-bolometer can be used effectively for LIT. The performance of the thermal core is quantitatively compared with the more expensive and bulky traditional infra-red cameras. It is shown that the thermal core can detect defects with similar overall performance as a cooled photon detector and an uncooled micro-bolometer. The low-cost, compact nature and small mass of the thermal core offers great potential in thermographic inspection opening the possibility of deploying devices permanently on structures in conjunction with the embedded actuators for in-situ monitoring in the service environment.

year: 2022

title: Caldolase, a chelator-insensitive extracellular serine proteinase from a Thermus spp.

authors: Saravani, G.A.

subjects: biology

abstract: An extracellular alkaline serine proteinase from Thermus strain ToK3 was isolated and purified to homogeneity by (NH4)2SO4 precipitation followed by ion-exchange chromatography on DEAE-cellulose and QAE-Sephadex, affinity chromatography on N alpha-benzyloxycarbonyl-D-phenylalanyl-triethylenetetraminyl-Sepha rose 4B and gel-filtration chromatography on Sephadex G-75. The purified enzyme had a pI of 8.9 and an Mr determined by gel-permeation chromatography of 25,000. The specific activity was about 37,700 proteolytic units/mg with casein as substrate, and the pH optimum was 9.5. Proteolytic activity was inhibited by low concentrations of di-isopropyl phosphorofluoridate and phenylmethanesulphonyl fluoride, but was unaffected by EDTA, EGTA, o-phenanthroline, N-ethyl-5-phenylisoxazolium-3'-sulphonate, N alpha-p-tosyl-L-phenylalanylchloromethane, N alpha-p-tosyl-L-lysylchloromethane, trypsin inhibitors and pepstatin A. The enzyme contained approx. 10% carbohydrate and four disulphide bonds. No Ca2+, Zn2+ or free thiol groups were detected. It hydrolysed several native and dye-linked proteins and synthetic chromogenic peptides and esters. The enzyme was very thermostable (half-life values were 840 min at 80 degrees C, 45 min at 90 degrees C and 5 min at 100 degrees C). The enzyme was unstable at low ionic strength: after 60 min at 75 degrees C in 0.1 M-Tris/acetate buffer, pH 8, only 20% activity remained, compared with no loss in 0.1 M-Tris/acetate buffer, pH 8, containing 0.4 M-NaCl.

text: Caldolase, a chelator-insensitive extracellular serine proteinase from a Thermus spp. An extracellular alkaline serine proteinase from Thermus strain ToK3 was isolated and purified to homogeneity by (NH4)2SO4 precipitation followed by ion-exchange chromatography on DEAE-cellulose and QAE-Sephadex, affinity chromatography on N alpha-benzyloxycarbonyl-D-phenylalanyl-triethylenetetraminyl-Sepha rose 4B and gel-filtration chromatography on Sephadex G-75. The purified enzyme had a pI of 8.9 and an Mr determined by gel-permeation chromatography of 25,000. The specific activity was about 37,700 proteolytic units/mg with casein as substrate, and the pH optimum was 9.5. Proteolytic activity was inhibited by low concentrations of di-isopropyl phosphorofluoridate and phenylmethanesulphonyl fluoride, but was unaffected by EDTA, EGTA, o-phenanthroline, N-ethyl-5-phenylisoxazolium-3'-sulphonate, N alpha-p-tosyl-L-phenylalanylchloromethane, N alpha-p-tosyl-L-lysylchloromethane, trypsin inhibitors and pepstatin A. The enzyme contained approx. 10% carbohydrate and four disulphide bonds. No Ca2+, Zn2+ or free thiol groups were detected. It hydrolysed several native and dye-linked proteins and synthetic chromogenic peptides and esters. The enzyme was very thermostable (half-life values were 840 min at 80 degrees C, 45 min at 90 degrees C and 5 min at 100 degrees C). The enzyme was unstable at low ionic strength: after 60 min at 75 degrees C in 0.1 M-Tris/acetate buffer, pH 8, only 20% activity remained, compared with no loss in 0.1 M-Tris/acetate buffer, pH 8, containing 0.4 M-NaCl.

year: 1989

title: Te Mata Ono. E tipu atu te kūmara, e ohu mai te anuhe

authors: Pēwhairangi, Te Aorere Tūwhakairiora

subjects: Māori television

abstract: Mai anō, mai anō kua whakapaoho te iwi Māori, tīmata mai ai i te mahi whaikōrero, i te mahi karanga, i te mahi taonga puoro, i te mahi toi, me te mahi waiata. Ko ngā mahi nei he huarahi tuku karere i tētahi takitahi, takitini rānei ki tētahi takitahi, takitini rānei. Mā ēnei mahi e whakawhitiwhiti mōhiohio ai te tangata. Nō te taitanga mai a te Pākehā kua whai huarahi whakapaoho atu anō te iwi Māori i ō rātau mōhiohio, tīmata ana mai i ngā niu pepa, eke ana rā ki ngā reo irirangi, tae noa mai ki te pouaka whakaata me te ipurangi. Ko te rangahautanga nei ka titiro ki tētahi o ngā huarahi mātāmuri e whakapaohotia ai ngā mōhiohio a te Māori, arā te pouaka whakaata. Ka arotaketia ngā mahi tuari mōhiohio a te whare waihanga hōtaka pouaka whakaata nei a Whakaata Māori mā tētahi anga tātai e kīia nei ko Te Mata Ono.

I ngā tau 15 kua pahure ake nei ehara i te mea kua tū a Whakaata Māori hai kaitohatoha mōhiohio Māori, engari hai kaiwhakarauora hoki i te reo Māori me ngā tikanga Māori. I te tuhinga roa nei ka tirohia ngā huarahi taketake me ngā huarahi hōu o te whakapaoho i ngā mōhiohio o te tangata mā roto i a Whakaata Māori. Mā te whakauruuru i ngā huarahi e rua nei e whakahōuhia ai te ara, e whakapaoho Māoritia ai hoki ngā hōtaka o te wā. 

E ono katoa ngā āhuatanga o te anga e kōrerotia nei. Ko te tuhinga roa nei he haurua o te anga. Nā ngā ture ā-kupu o te tohu paerua nei, ka rangahaua rētōtia e a au ngā wāhanga e toru o te ono e kīia nei ko Te Mata Ako, Te Mata Tikanga, Te Mata Iwi anō hoki. E tuhia ana hoki tētahi atu tuhinga roa i te wā nei e Kimiora Kaire-Melbourne. Kai te titiro ia ki ērā atu wāhanga e toru o te anga, Te Mata Māori, Te Mata Pākehā me Te Mata ō-Tua. Hai te mutunga o ngā tuhinga roa e rua nei, ka whakapiria ai ngā āhuatanga e ono kia waihanga pūmautia ai te anga tātari o Te Mata Ono. Ka piri ana te anga tātari ko te whāinga kia whakahāngaitia ai ki a Whakaata Māori hai tautoko, hai hāpai, hai ārahi hoki i te teihanga ki ngā rā kai te heke mai.

text: Te Mata Ono. E tipu atu te kūmara, e ohu mai te anuhe Mai anō, mai anō kua whakapaoho te iwi Māori, tīmata mai ai i te mahi whaikōrero, i te mahi karanga, i te mahi taonga puoro, i te mahi toi, me te mahi waiata. Ko ngā mahi nei he huarahi tuku karere i tētahi takitahi, takitini rānei ki tētahi takitahi, takitini rānei. Mā ēnei mahi e whakawhitiwhiti mōhiohio ai te tangata. Nō te taitanga mai a te Pākehā kua whai huarahi whakapaoho atu anō te iwi Māori i ō rātau mōhiohio, tīmata ana mai i ngā niu pepa, eke ana rā ki ngā reo irirangi, tae noa mai ki te pouaka whakaata me te ipurangi. Ko te rangahautanga nei ka titiro ki tētahi o ngā huarahi mātāmuri e whakapaohotia ai ngā mōhiohio a te Māori, arā te pouaka whakaata. Ka arotaketia ngā mahi tuari mōhiohio a te whare waihanga hōtaka pouaka whakaata nei a Whakaata Māori mā tētahi anga tātai e kīia nei ko Te Mata Ono.

I ngā tau 15 kua pahure ake nei ehara i te mea kua tū a Whakaata Māori hai kaitohatoha mōhiohio Māori, engari hai kaiwhakarauora hoki i te reo Māori me ngā tikanga Māori. I te tuhinga roa nei ka tirohia ngā huarahi taketake me ngā huarahi hōu o te whakapaoho i ngā mōhiohio o te tangata mā roto i a Whakaata Māori. Mā te whakauruuru i ngā huarahi e rua nei e whakahōuhia ai te ara, e whakapaoho Māoritia ai hoki ngā hōtaka o te wā. 

E ono katoa ngā āhuatanga o te anga e kōrerotia nei. Ko te tuhinga roa nei he haurua o te anga. Nā ngā ture ā-kupu o te tohu paerua nei, ka rangahaua rētōtia e a au ngā wāhanga e toru o te ono e kīia nei ko Te Mata Ako, Te Mata Tikanga, Te Mata Iwi anō hoki. E tuhia ana hoki tētahi atu tuhinga roa i te wā nei e Kimiora Kaire-Melbourne. Kai te titiro ia ki ērā atu wāhanga e toru o te anga, Te Mata Māori, Te Mata Pākehā me Te Mata ō-Tua. Hai te mutunga o ngā tuhinga roa e rua nei, ka whakapiria ai ngā āhuatanga e ono kia waihanga pūmautia ai te anga tātari o Te Mata Ono. Ka piri ana te anga tātari ko te whāinga kia whakahāngaitia ai ki a Whakaata Māori hai tautoko, hai hāpai, hai ārahi hoki i te teihanga ki ngā rā kai te heke mai.

year: 2019