title: Chemical ecological insights into metabolites of the New Zealand marine sponge, Cliona celata

authors: Webb, Nicola G.

subjects: chemical ecology

abstract: Marine sponges are known to produce secondary metabolites that have roles including chemical defence against cellular challenge. An investigation into Cliona celata around the Bay of Plenty (New Zealand) was conducted in response to discovery of unusual metabolites that had the potential to be biologically active. The study was designed to determine chemical variation in the sponge over small spatial and temporal scales and to determine the possible ecological triggers for the presence of these metabolites. The hypothesis was that a chemical defensive response would be elicited 24-48 hours post a cellular challenge event such as physical damage. The hypothesis was based on the fact that the sponge heals quickly when damaged with little evidence of necrosis.

Cliona celata was chosen as there is little knowledge of the species within New Zealand, and previous work showed a single specimen to produce brominated compounds, never before reported for this genus. This project examines whether the production of brominated compounds is common throughout all C. celata within the Bay of Plenty region or is a localised response.

A preliminary study was conducted to investigate spatial chemical variability of sponges from three locations, Leisure Island (intertidal), Rabbit Island (subtidal) (both on the coast adjacent to Mount Maunganui, Tauranga) and White Island (geothermal subtidal). A damage response experiment for Leisure Island and Rabbit Island specimens was conducted to determine chemical differences in healthy versus damaged sponges, and whether a chemical defence mechanism was utilised by the sponge species. Lastly, a stratification experiment was performed to establish whether chemical changes occur throughout the sponge or are localised to the immediate damage site.

Cliona celata identification was confirmed using spicule mounts. However, some variation between intertidal and subtidal specimens was noted with differences in spicular morphology, likely due to variations in chemistry and micro-environments. 

On a small scale, extracts of each sample were prepared and analysed using Liquid Chromatography Mass Spectrometry (LCMS). Damaged and non damaged sponge specimens were subjected to an MTT assay using the human cervical cancer cell line (HeLa). But the crude extracts showed no bioactivity in this assay. 

Chromatograms obtained from LCMS, for all samples contained a common set of peaks, however, variations in intensities and some minor peak variations within the sites (intertidal, subtidal and geothermal subtidal) and individual specimens were noted, and could represent interactions with different micro-environments. A lack of chemical response to damage was not expected. It is therefore hypothesised that the lack of secondary metabolite production observed, is due to cells reverting to archaeocytes to repair and re-grow the removed sponge section, a histological response rather than a chemical one. 

It was hypothesised that specimens with increased levels of epibiont coverage had increased production of certain metabolites, as represented by increased intensities of some peaks in chromatograms, however, to a threshold. Once epibiont coverage of approximately 50% was met, novel chemicals were thought to be produced by the sponge. Two sponge samples did produce monobrominated and dibrominated compounds, however, these were localised events and could potentially be the response to an unidentified external stressor or epibiont coverage. The stratification experiment indicated that the isolation and production of these brominated compounds may be restricted to the pinacoderm of the sponge, possibly suggesting a transient chemical response as cells re-organise themselves in a healing process. However, further investigation is required with all experiments and a more comprehensive time series of samples needs to be taken post damage treatment.
This project provides a preliminary study into the chemical variation and chemical response to damage for intertidal and subtidal Bay of Plenty Cliona celata. Any hypotheses made during this thesis need to be further examined to be accepted.

text: Chemical ecological insights into metabolites of the New Zealand marine sponge, Cliona celata Marine sponges are known to produce secondary metabolites that have roles including chemical defence against cellular challenge. An investigation into Cliona celata around the Bay of Plenty (New Zealand) was conducted in response to discovery of unusual metabolites that had the potential to be biologically active. The study was designed to determine chemical variation in the sponge over small spatial and temporal scales and to determine the possible ecological triggers for the presence of these metabolites. The hypothesis was that a chemical defensive response would be elicited 24-48 hours post a cellular challenge event such as physical damage. The hypothesis was based on the fact that the sponge heals quickly when damaged with little evidence of necrosis.

Cliona celata was chosen as there is little knowledge of the species within New Zealand, and previous work showed a single specimen to produce brominated compounds, never before reported for this genus. This project examines whether the production of brominated compounds is common throughout all C. celata within the Bay of Plenty region or is a localised response.

A preliminary study was conducted to investigate spatial chemical variability of sponges from three locations, Leisure Island (intertidal), Rabbit Island (subtidal) (both on the coast adjacent to Mount Maunganui, Tauranga) and White Island (geothermal subtidal). A damage response experiment for Leisure Island and Rabbit Island specimens was conducted to determine chemical differences in healthy versus damaged sponges, and whether a chemical defence mechanism was utilised by the sponge species. Lastly, a stratification experiment was performed to establish whether chemical changes occur throughout the sponge or are localised to the immediate damage site.

Cliona celata identification was confirmed using spicule mounts. However, some variation between intertidal and subtidal specimens was noted with differences in spicular morphology, likely due to variations in chemistry and micro-environments. 

On a small scale, extracts of each sample were prepared and analysed using Liquid Chromatography Mass Spectrometry (LCMS). Damaged and non damaged sponge specimens were subjected to an MTT assay using the human cervical cancer cell line (HeLa). But the crude extracts showed no bioactivity in this assay. 

Chromatograms obtained from LCMS, for all samples contained a common set of peaks, however, variations in intensities and some minor peak variations within the sites (intertidal, subtidal and geothermal subtidal) and individual specimens were noted, and could represent interactions with different micro-environments. A lack of chemical response to damage was not expected. It is therefore hypothesised that the lack of secondary metabolite production observed, is due to cells reverting to archaeocytes to repair and re-grow the removed sponge section, a histological response rather than a chemical one. 

It was hypothesised that specimens with increased levels of epibiont coverage had increased production of certain metabolites, as represented by increased intensities of some peaks in chromatograms, however, to a threshold. Once epibiont coverage of approximately 50% was met, novel chemicals were thought to be produced by the sponge. Two sponge samples did produce monobrominated and dibrominated compounds, however, these were localised events and could potentially be the response to an unidentified external stressor or epibiont coverage. The stratification experiment indicated that the isolation and production of these brominated compounds may be restricted to the pinacoderm of the sponge, possibly suggesting a transient chemical response as cells re-organise themselves in a healing process. However, further investigation is required with all experiments and a more comprehensive time series of samples needs to be taken post damage treatment.
This project provides a preliminary study into the chemical variation and chemical response to damage for intertidal and subtidal Bay of Plenty Cliona celata. Any hypotheses made during this thesis need to be further examined to be accepted.

year: 2015

title: Higher education and the Anthropocene - Towards an ecological approach to higher education policy in New Zealand

authors: Stratford, Robert James

subjects: Anthropocene

abstract: In this thesis I present an ecological direction for higher education policy in Aotearoa/New Zealand. This position is developed through an ecological approach to policy, which includes a postfoundational take on ecological theory, especially the work of Gregory Bateson and Felix Guattari. This ecological approach to higher education policy is in contrast to the neoliberal and technicist policy thinking which has informed New Zealand’s Tertiary Education Strategy (Ministry of Education, 2014c). As a contrast, the ecological approach in this thesis draws strength from ecological economics, environmental politics, critical policy analysis, ecological theory and philosophical pragmatism. The methodological core of this approach is described as Critical Eco Pragmatism (CEP). Following a discussion of ecological theory and an exploration of the Global Ecological Crisis (GEC) as an interconnected problem of natural, political, social, psychological, pedagogical and epistemological dimensions, I develop a theoretical framework for being ecological in higher education. This framework draws on a critique of Ron Barnett’s work on the ecological university (Barnett, 2010, 2018) and introduces the notion of ‘Anthropocene Intelligence’. Anthropocene Intelligence provides a way to pragmatically bring together a range of theoretical ideas about education – especially those ideas that have a claim on improving our psychological, social and natural ecologies. This includes educational discourses that have not always had a high level of interaction, such as environmental and sustainability education (ESE), indigenous education, eco-pedagogy, engaged scholarship, ecological humanities, human development education, and education for wellbeing (including the healthy university). The potential of an ecological approach is also considered in relation to the many practical possibilities that currently exist in higher education policy and practice both internationally and in New Zealand. Together with the theoretical approach taken in this thesis, these practical possibilities inform the alternative, ecological direction this thesis develops for higher education policy in New Zealand. Included in this ecological direction is the aspiration for New Zealand to develop as an ‘ecological democracy’ (Dryzek, 2013).

text: Higher education and the Anthropocene - Towards an ecological approach to higher education policy in New Zealand In this thesis I present an ecological direction for higher education policy in Aotearoa/New Zealand. This position is developed through an ecological approach to policy, which includes a postfoundational take on ecological theory, especially the work of Gregory Bateson and Felix Guattari. This ecological approach to higher education policy is in contrast to the neoliberal and technicist policy thinking which has informed New Zealand’s Tertiary Education Strategy (Ministry of Education, 2014c). As a contrast, the ecological approach in this thesis draws strength from ecological economics, environmental politics, critical policy analysis, ecological theory and philosophical pragmatism. The methodological core of this approach is described as Critical Eco Pragmatism (CEP). Following a discussion of ecological theory and an exploration of the Global Ecological Crisis (GEC) as an interconnected problem of natural, political, social, psychological, pedagogical and epistemological dimensions, I develop a theoretical framework for being ecological in higher education. This framework draws on a critique of Ron Barnett’s work on the ecological university (Barnett, 2010, 2018) and introduces the notion of ‘Anthropocene Intelligence’. Anthropocene Intelligence provides a way to pragmatically bring together a range of theoretical ideas about education – especially those ideas that have a claim on improving our psychological, social and natural ecologies. This includes educational discourses that have not always had a high level of interaction, such as environmental and sustainability education (ESE), indigenous education, eco-pedagogy, engaged scholarship, ecological humanities, human development education, and education for wellbeing (including the healthy university). The potential of an ecological approach is also considered in relation to the many practical possibilities that currently exist in higher education policy and practice both internationally and in New Zealand. Together with the theoretical approach taken in this thesis, these practical possibilities inform the alternative, ecological direction this thesis develops for higher education policy in New Zealand. Included in this ecological direction is the aspiration for New Zealand to develop as an ‘ecological democracy’ (Dryzek, 2013).

year: 2019

title: Researching the Process of Auahi Kore for Marae

authors: Masters-Awatere, Bridgette

abstract: • The current report is piece of a qualitative research exploring issues relating the development of Marae Auahi Kore. Such aspects as; factors that aid or impede the process, resources available or required by marae, and people's perceptions of the Auahi Kore brand were explored.

• Interviews were conducted with people from a variety of backgrounds. Research participants included: Marae Trustees, Public Health Workers, Runanga Staff as well as Kaumatua and Kuia. These people were identified as key informants for the current project. A total of 25 people were interviewed during March and April using an open interview schedule.

• Exploration into the Smokefree policies of marae was not pursued within the current project as this was not the focus nor the intent of the research. Project constraints such as time and budget meant there was a limit to what could be undertaken for the current piece of research.

text: Researching the Process of Auahi Kore for Marae • The current report is piece of a qualitative research exploring issues relating the development of Marae Auahi Kore. Such aspects as; factors that aid or impede the process, resources available or required by marae, and people's perceptions of the Auahi Kore brand were explored.

• Interviews were conducted with people from a variety of backgrounds. Research participants included: Marae Trustees, Public Health Workers, Runanga Staff as well as Kaumatua and Kuia. These people were identified as key informants for the current project. A total of 25 people were interviewed during March and April using an open interview schedule.

• Exploration into the Smokefree policies of marae was not pursued within the current project as this was not the focus nor the intent of the research. Project constraints such as time and budget meant there was a limit to what could be undertaken for the current piece of research.

year: 2000