title: Fine-scale cryogenic sampling of planktonic microbial communities: Application to toxic cyanobacterial blooms

authors: Puddick, Jonathan

abstract: A lack of fine-scale methods for sampling planktonic microbial populations hinders advancement in understanding the responses of these communities to environmental conditions. Current methods provide resolution at scales of centimeters to meters, but not at the millimeter-scale required to understand highly stratified communities. To address this we developed two cryogenic sampling tools to collect spatially-precise samples from aquatic environments while simultaneously preserving the microbial communities. The application of these samplers was examined over a 5.5 h period using a cyanobacterial scum (Microcystis) formed in experimental mesocosms. A cryogenic “surface snatcher” collected a discrete layer (ca. 1 mm) of surface water. Compared to conventional surface sampling methods, the surface snatcher samples contained up to 22-times more microcystin, indicating that less underlying water was incorporated into the sample. A cryogenic “cold finger” sampler was used to collect vertical profiles of the upper 40 mm of the water column. This profiler provided new insights into the fine-scale structure of Microcystis scums, demonstrating that more microcystin-producing Microcystis was contained in the surface 5 mm than the 35 mm below. The results also showed that upregulation of microcystin production was highly localized in the top 2.5 mm of the Microcystis scum. Our results demonstrate that extreme changes in cyanobacterial communities can occur over small distances, and indicate that sampling resolution is of great importance for improving knowledge on cyanobacterial blooms and toxin production. While this study focused on microcystin-producing Microcystis, the cryogenic sampling tools described here could be applied to any planktonic microbial community.

text: Fine-scale cryogenic sampling of planktonic microbial communities: Application to toxic cyanobacterial blooms A lack of fine-scale methods for sampling planktonic microbial populations hinders advancement in understanding the responses of these communities to environmental conditions. Current methods provide resolution at scales of centimeters to meters, but not at the millimeter-scale required to understand highly stratified communities. To address this we developed two cryogenic sampling tools to collect spatially-precise samples from aquatic environments while simultaneously preserving the microbial communities. The application of these samplers was examined over a 5.5 h period using a cyanobacterial scum (Microcystis) formed in experimental mesocosms. A cryogenic “surface snatcher” collected a discrete layer (ca. 1 mm) of surface water. Compared to conventional surface sampling methods, the surface snatcher samples contained up to 22-times more microcystin, indicating that less underlying water was incorporated into the sample. A cryogenic “cold finger” sampler was used to collect vertical profiles of the upper 40 mm of the water column. This profiler provided new insights into the fine-scale structure of Microcystis scums, demonstrating that more microcystin-producing Microcystis was contained in the surface 5 mm than the 35 mm below. The results also showed that upregulation of microcystin production was highly localized in the top 2.5 mm of the Microcystis scum. Our results demonstrate that extreme changes in cyanobacterial communities can occur over small distances, and indicate that sampling resolution is of great importance for improving knowledge on cyanobacterial blooms and toxin production. While this study focused on microcystin-producing Microcystis, the cryogenic sampling tools described here could be applied to any planktonic microbial community.

year: 2016

title: The proposal for a third medical school in New Zealand: A community-engaged graduate entry medical program

authors: Lawrenson, Ross

abstract: New Zealand has a maldistributed workforce that is heavily dependent on recruiting international medical graduates. Shortages are particularly apparent in high needs communities and in general scope specialties in provincial regions. The University of Waikato in partnership with the Waikato District Health Board has proposed a third medical school for New Zealand which will concentrate on addressing the workforce needs of disadvantaged rural and provincial communities. The proposed program is a community engaged, graduate entry medical course.

text: The proposal for a third medical school in New Zealand: A community-engaged graduate entry medical program New Zealand has a maldistributed workforce that is heavily dependent on recruiting international medical graduates. Shortages are particularly apparent in high needs communities and in general scope specialties in provincial regions. The University of Waikato in partnership with the Waikato District Health Board has proposed a third medical school for New Zealand which will concentrate on addressing the workforce needs of disadvantaged rural and provincial communities. The proposed program is a community engaged, graduate entry medical course.

year: 2017-04-07

title: Te reo Maori, philosophy and colonialism: A conversation with Maori philosopher Carl Mika

authors: Peters, Michael A.

abstract: Kia ora Carl
Some advice if you have the time... I have been asked to give a paper at Uppsala on philosophy of language and autonomy. I want to focus on colonialism and use some examples from the colonial history concerning te reo Maori. I did a little research at the time of the Royal Commission in 1988 but have lost touch with the literature. Are there a couple of strategic texts you would recommend?
Nga Mihi

Kia ora Michael
You tend to get two types of writing about the language: its revitalisation; and its link to the natural or spiritual worlds – which is to do with philosophy in a particular sense, but in my view doesn’t have an eye towards the “autonomy” part you raise (i.e. isn’t cognisant enough of colonialism). The latter writing theorises around the traditional place of language, or describes it as a traditional phenomenon. I’ve been considering writing something for some time on it, but just haven’t gotten around to it.
You could discuss it in terms of how current uses of it in government policy etc. force the Maori language to become no more or no less than its English counterpart. So, for instance, language is an arbitrary (Saussure) thing that has very little in the way of “essence” in its own (autonomous) right. Terms like “whakapapa” equate precisely with “genealogy” but their interconnecting sense is lost in that translation. From a Maori belief, it could be argued that everything contains an essence, including words, and this essence precedes our interaction with language. In a way, language very much opens up a worldview, including the autonomy or essence of things in the world and their interconnectedness. pp. 101–110

text: Te reo Maori, philosophy and colonialism: A conversation with Maori philosopher Carl Mika Kia ora Carl
Some advice if you have the time... I have been asked to give a paper at Uppsala on philosophy of language and autonomy. I want to focus on colonialism and use some examples from the colonial history concerning te reo Maori. I did a little research at the time of the Royal Commission in 1988 but have lost touch with the literature. Are there a couple of strategic texts you would recommend?
Nga Mihi

Kia ora Michael
You tend to get two types of writing about the language: its revitalisation; and its link to the natural or spiritual worlds – which is to do with philosophy in a particular sense, but in my view doesn’t have an eye towards the “autonomy” part you raise (i.e. isn’t cognisant enough of colonialism). The latter writing theorises around the traditional place of language, or describes it as a traditional phenomenon. I’ve been considering writing something for some time on it, but just haven’t gotten around to it.
You could discuss it in terms of how current uses of it in government policy etc. force the Maori language to become no more or no less than its English counterpart. So, for instance, language is an arbitrary (Saussure) thing that has very little in the way of “essence” in its own (autonomous) right. Terms like “whakapapa” equate precisely with “genealogy” but their interconnecting sense is lost in that translation. From a Maori belief, it could be argued that everything contains an essence, including words, and this essence precedes our interaction with language. In a way, language very much opens up a worldview, including the autonomy or essence of things in the world and their interconnectedness. pp. 101–110

year: 2015