title: Advantages of 3D time-of-flight range imaging cameras in machine vision applications

authors: Dorrington, Adrian A.

subjects: range imaging

abstract: Machine vision using image processing of traditional intensity images is in wide spread use. In many situations environmental conditions or object colours or shades cannot be controlled, leading to difficulties in correctly processing the images and requiring complicated processing algorithms. Many of these complications can be avoided by using range image data, instead of intensity data. This is because range image data represents the physical properties of object location and shape, practically independently of object colour or shading. The advantages of range image processing are presented, along with three example applications that show how robust machine vision results can be obtained with relatively simple range image processing in real-time applications.

text: Advantages of 3D time-of-flight range imaging cameras in machine vision applications Machine vision using image processing of traditional intensity images is in wide spread use. In many situations environmental conditions or object colours or shades cannot be controlled, leading to difficulties in correctly processing the images and requiring complicated processing algorithms. Many of these complications can be avoided by using range image data, instead of intensity data. This is because range image data represents the physical properties of object location and shape, practically independently of object colour or shading. The advantages of range image processing are presented, along with three example applications that show how robust machine vision results can be obtained with relatively simple range image processing in real-time applications.

year: 2009

title: Toward catalytic antibodies capable of ergopeptine hydrolysis

authors: Dines, Mark

abstract: Animals grazing endophyte infected pasture are exposed to the toxic effects of ergopeptine compounds which are produced by the grass-endophyte interaction. Grasses containing endophytic fungi have a competitive advantage and comprise over 60% of pastureland. Catalytic antibody vaccines would permit the use of endophyte-infected grasses without the risk of animal toxicoses. The production of catalytic antibodies capable of hydrolysing ergopeptine toxins was investigated. The literature pertaining to catalytic antibodies is reviewed with particular focus on hydrolytic reactions. Model systems were used to investigate the potential of this technique.

Model phosphorus-ester and -amide compounds were synthesised and characterised. Problems were experienced forming the RPO(OCH₂CH₃)X where X is NH(C₆H₄NO₂), and R is a protected benzyl system, successful generation of model compounds where X is NHCH₂(C₆H₅) and O(C₆H₄NO₂) was achieved. The compound HOOCCH₂CH₂CONH(C₆H₄)CH₂PO(OH)O(C₆H₄NO₂) was synthesised as a transition-state emulator for the generation of polyclonal antibodies capable of hydrolysing (C₆H₅)CH₂COO(C₆H₄NO₂) and (C₆H₅)CH₂CONH(C₆H₄NO₂). The transition-state emulator was conjugated to Bovine Serum Albumin, Thyroglobulin and Keyhole Limpet Haemocyanin. Sheep, mice and a rabbit were immunised with the resulting conjugate material.

The immune rabbit serum was found to rapidly hydrolyse ester ((C₆H₅)CH₂COO(C₆H₄NO₂)) relative to the non-immune serum, however, purification of the immune rabbit antibodies indicated that the hydrolysis of ester ((C₆H₅)CH₂COO(C₆H₄NO₂)) was not due to antibody catalysis. The non-purified sera from the immune sheep and mice was found to hydrolyse ester ((C₆H₅)CH₂COO(C₆H₄NO₂)) at a similar rate to the non-immune sera and no immune sera was found to hydrolyse amide ((C₆H₅)CH₂CONH(C₆H₄NO₂)). Investigation of sera from immunised animals by ELISA indicated production of polyclonal antibodies capable of binding the HOOCCH₂CH₂CONH(C₆H₄)CH₂PO(OH)O(C₆H₄NO₂) material conjugated to protein. This protein was different to that used as immunogen. However, the non-conjugated HOOCCH₂CH₂CONH(C₆H₄)CH₂PO(OH)O(C₆H₄NO₂) material did not competitively inhibit polyclonal antibody binding to the protein conjugates, suggesting that the polyclonal antibodies generated required the presence of a conjugated protein environment for binding.
 
An investigation of the ergot alkaloids produced by one grass-endophyte association (Acnatherum inebrians) known to contain high levels of ergot alkaloid was conducted. Extraction methodology and reverse phase HPLC separation systems were developed for these compounds. Use of these methods enabled the purification of iso-lysergic acid amide and ergonovinine from Acnatherum inebrians. These compounds were characterised using NMR.

This thesis details the generation of a model transition-state compound and subsequent immunisation of this compound into animals. Antibodies from the immune animals were not found to catalyse the hydrolysis of amide or ester bonds. This work and the work of others suggests that a better understanding of the emulation of the amide transition-state is required before detoxification of ergopeptines by amide hydrolysising antibodies will be achieved.

text: Toward catalytic antibodies capable of ergopeptine hydrolysis Animals grazing endophyte infected pasture are exposed to the toxic effects of ergopeptine compounds which are produced by the grass-endophyte interaction. Grasses containing endophytic fungi have a competitive advantage and comprise over 60% of pastureland. Catalytic antibody vaccines would permit the use of endophyte-infected grasses without the risk of animal toxicoses. The production of catalytic antibodies capable of hydrolysing ergopeptine toxins was investigated. The literature pertaining to catalytic antibodies is reviewed with particular focus on hydrolytic reactions. Model systems were used to investigate the potential of this technique.

Model phosphorus-ester and -amide compounds were synthesised and characterised. Problems were experienced forming the RPO(OCH₂CH₃)X where X is NH(C₆H₄NO₂), and R is a protected benzyl system, successful generation of model compounds where X is NHCH₂(C₆H₅) and O(C₆H₄NO₂) was achieved. The compound HOOCCH₂CH₂CONH(C₆H₄)CH₂PO(OH)O(C₆H₄NO₂) was synthesised as a transition-state emulator for the generation of polyclonal antibodies capable of hydrolysing (C₆H₅)CH₂COO(C₆H₄NO₂) and (C₆H₅)CH₂CONH(C₆H₄NO₂). The transition-state emulator was conjugated to Bovine Serum Albumin, Thyroglobulin and Keyhole Limpet Haemocyanin. Sheep, mice and a rabbit were immunised with the resulting conjugate material.

The immune rabbit serum was found to rapidly hydrolyse ester ((C₆H₅)CH₂COO(C₆H₄NO₂)) relative to the non-immune serum, however, purification of the immune rabbit antibodies indicated that the hydrolysis of ester ((C₆H₅)CH₂COO(C₆H₄NO₂)) was not due to antibody catalysis. The non-purified sera from the immune sheep and mice was found to hydrolyse ester ((C₆H₅)CH₂COO(C₆H₄NO₂)) at a similar rate to the non-immune sera and no immune sera was found to hydrolyse amide ((C₆H₅)CH₂CONH(C₆H₄NO₂)). Investigation of sera from immunised animals by ELISA indicated production of polyclonal antibodies capable of binding the HOOCCH₂CH₂CONH(C₆H₄)CH₂PO(OH)O(C₆H₄NO₂) material conjugated to protein. This protein was different to that used as immunogen. However, the non-conjugated HOOCCH₂CH₂CONH(C₆H₄)CH₂PO(OH)O(C₆H₄NO₂) material did not competitively inhibit polyclonal antibody binding to the protein conjugates, suggesting that the polyclonal antibodies generated required the presence of a conjugated protein environment for binding.
 
An investigation of the ergot alkaloids produced by one grass-endophyte association (Acnatherum inebrians) known to contain high levels of ergot alkaloid was conducted. Extraction methodology and reverse phase HPLC separation systems were developed for these compounds. Use of these methods enabled the purification of iso-lysergic acid amide and ergonovinine from Acnatherum inebrians. These compounds were characterised using NMR.

This thesis details the generation of a model transition-state compound and subsequent immunisation of this compound into animals. Antibodies from the immune animals were not found to catalyse the hydrolysis of amide or ester bonds. This work and the work of others suggests that a better understanding of the emulation of the amide transition-state is required before detoxification of ergopeptines by amide hydrolysising antibodies will be achieved.

year: 1998

title: Differential trends in the compression of mortality: Assessing the antecedents to current gaps in health expectancy in New Zealand

authors: Pool, Ian

subjects: New Zealand

abstract: Health Expectancies (HEs) for New Zealand show significant differentials between Maori and non-Maori, but also by gender and period. These differentials correlate with findings from both generation and synthetic life-tables relating to New Zealand’s epidemiologic transition. At the beginning of that transition quartile 1 (Q(1)), and Median (Med) d(x) values were close and centred at young ages; during the transition the gap became very wide; at the transition’s end the gap again narrowed. Cohort and synthetic trends in d(x), l(x), M, Qs and Meds are reviewed and linked to recent HEs. Data point to epidemic polarisation. Cohort analysis allows the evaluation of the role of past experiences on the recent HEs, and thus point to possible strategies for reducing gaps in both d(x), and HEs.

text: Differential trends in the compression of mortality: Assessing the antecedents to current gaps in health expectancy in New Zealand Health Expectancies (HEs) for New Zealand show significant differentials between Maori and non-Maori, but also by gender and period. These differentials correlate with findings from both generation and synthetic life-tables relating to New Zealand’s epidemiologic transition. At the beginning of that transition quartile 1 (Q(1)), and Median (Med) d(x) values were close and centred at young ages; during the transition the gap became very wide; at the transition’s end the gap again narrowed. Cohort and synthetic trends in d(x), l(x), M, Qs and Meds are reviewed and linked to recent HEs. Data point to epidemic polarisation. Cohort analysis allows the evaluation of the role of past experiences on the recent HEs, and thus point to possible strategies for reducing gaps in both d(x), and HEs.

year: 2009