title: The Purification of Cellulase and Hemicellulase Components from an Extreme Thermophile by the Cloning of Enzymes into E. coli

authors: Schofield, L.R.

subjects: biology

abstract: The use of heat treatment to purify enzymes by selective denaturation and then by the subsequent precipitation of denatured protein is a simple, rapid, and well established procedure. Successful applications are limited to those few enzymes that possess a thermostability considerably higher than the majority of cell proteins. The introduction of thermostable enzymes into the protein population of a mesophile by cloning offers a clear opportunity to employ a heat-treatment method of purification to its full advantage (e.g., see references 1 and 2).
In light of the difficulties involved in purifying bacterial cellulases, the cloning of some of the cellulase and hemicellulase genes of Caldocellum saccharolyticum into Escherichia coli has provided a welcome alternative procedure for obtaining pure enzymes.

text: The Purification of Cellulase and Hemicellulase Components from an Extreme Thermophile by the Cloning of Enzymes into E. coli The use of heat treatment to purify enzymes by selective denaturation and then by the subsequent precipitation of denatured protein is a simple, rapid, and well established procedure. Successful applications are limited to those few enzymes that possess a thermostability considerably higher than the majority of cell proteins. The introduction of thermostable enzymes into the protein population of a mesophile by cloning offers a clear opportunity to employ a heat-treatment method of purification to its full advantage (e.g., see references 1 and 2).
In light of the difficulties involved in purifying bacterial cellulases, the cloning of some of the cellulase and hemicellulase genes of Caldocellum saccharolyticum into Escherichia coli has provided a welcome alternative procedure for obtaining pure enzymes.

year: 1988

title: Optical measurement of blade edge sharpness

authors: Dredge, Daniel

subjects: Knife

abstract: This research aims to develop a superior way of characterizing knife sharpness. Current successful methods utilize contact destructive testing to develop a measurement protocol for sharpness however; it is in my opinion that testing in this manner, the instantaneous sharpness can never be known. Through-out this work, I investigate what physical properties determine sharpness, how failures occur in knives, the current state of art in knife sharpness testing, and then I seek to develop a new novel way of measuring knife sharpness through optical measurements.

text: Optical measurement of blade edge sharpness This research aims to develop a superior way of characterizing knife sharpness. Current successful methods utilize contact destructive testing to develop a measurement protocol for sharpness however; it is in my opinion that testing in this manner, the instantaneous sharpness can never be known. Through-out this work, I investigate what physical properties determine sharpness, how failures occur in knives, the current state of art in knife sharpness testing, and then I seek to develop a new novel way of measuring knife sharpness through optical measurements.

year: 2018

title: Systemic racism and oppression in psychology: Voices from psychologists, academic staff, and students

authors: Waitoki, Waikaremoana

subjects: psychology

abstract: Psychology must reassess its training approach, as the current monocultural paradigm not only causes harm, but also fails to equip psychologists with the knowledge needed to provide culturally appropriate care for Māori, Pacific, Asian, and other minoritised groups. Findings from this report contribute to the growing evidence of the urgent need to address barriers preventing Māori, Pacific, Asian, and other minoritised groups from participating effectively in professional psychology programmes. This 
report calls for action for everyone in psychology to embrace the changes needed to improve the cultural safety of the discipline and hold ourselves accountable for supporting the development of Te Tiriti-aligned psychology.

text: Systemic racism and oppression in psychology: Voices from psychologists, academic staff, and students Psychology must reassess its training approach, as the current monocultural paradigm not only causes harm, but also fails to equip psychologists with the knowledge needed to provide culturally appropriate care for Māori, Pacific, Asian, and other minoritised groups. Findings from this report contribute to the growing evidence of the urgent need to address barriers preventing Māori, Pacific, Asian, and other minoritised groups from participating effectively in professional psychology programmes. This 
report calls for action for everyone in psychology to embrace the changes needed to improve the cultural safety of the discipline and hold ourselves accountable for supporting the development of Te Tiriti-aligned psychology.

year: 2024