title: An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga.

authors: Simpson, H.D.

subjects: biology

abstract: Endo-1,4-beta-xylanase (EC 3.2.1.8) was isolated from the culture supernatant of Thermotoga sp. strain FjSS3-B.1, an extremely thermophilic anaerobic eubacterium which grows optimally at 80 degrees C. Activity was purified 165-fold by anion-exchange and hydroxyapatite chromatography. The enzyme has an Mr of 31,000 as determined by SDS/PAGE and 35,000 by analytical gel filtration. The optima for activity and stability for purified xylanase were between pH 5.0 and 5.5. At pH 5.5, which is the optimum pH for thermostability, t1/2 (95 degrees C) is 90 min. The thermostability was improved by immobilization of the xylanase on to porous glass beads; t1/2 (105 degrees C) is 10 min. Several additives, such as sorbitol and xylan, were also found to increase the thermostability. At 130 degrees C, the half-life of immobilized xylanase in the presence of 90% sorbitol was 1.3 min. At 130 degrees C in molten sorbitol half of the enzyme denatured rapidly, but the remainder appeared to have a half-life of about 60 min.

text: An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga. Endo-1,4-beta-xylanase (EC 3.2.1.8) was isolated from the culture supernatant of Thermotoga sp. strain FjSS3-B.1, an extremely thermophilic anaerobic eubacterium which grows optimally at 80 degrees C. Activity was purified 165-fold by anion-exchange and hydroxyapatite chromatography. The enzyme has an Mr of 31,000 as determined by SDS/PAGE and 35,000 by analytical gel filtration. The optima for activity and stability for purified xylanase were between pH 5.0 and 5.5. At pH 5.5, which is the optimum pH for thermostability, t1/2 (95 degrees C) is 90 min. The thermostability was improved by immobilization of the xylanase on to porous glass beads; t1/2 (105 degrees C) is 10 min. Several additives, such as sorbitol and xylan, were also found to increase the thermostability. At 130 degrees C, the half-life of immobilized xylanase in the presence of 90% sorbitol was 1.3 min. At 130 degrees C in molten sorbitol half of the enzyme denatured rapidly, but the remainder appeared to have a half-life of about 60 min.

year: 1991

title: Metabolic and performance responses of male runners wearing 3 types of footwear: Nike Vaporfly 4%, Saucony Endorphin racing flats, and their own shoes

authors: Hébert-Losier, Kim

subjects: Social Sciences

abstract: Purpose
We compared running economy (RE) and 3-km time-trial (TT) variables of runners wearing Nike Vaporfly 4% (VP4), Saucony Endorphin lightweight racing flats (FLAT), and their habitual running (OWN) footwear.

Methods
Eighteen male recreational runners (age = 33.5 ± 11.9 year (mean ± SD), peak oxygen uptake (VO2peak) = 55.8 ± 4.4 mL/kg·min) attended 4 sessions approximately 7 days apart. The first session consisted of a VO2peak test to inform subsequent RE speeds set at 60%, 70%, and 80% of the speed eliciting VO2peak. In subsequent sessions, treadmill RE and 3-km TTs were assessed in the 3 footwear conditions in a randomized, counterbalanced crossover design.

Results
Oxygen consumption (mL/kg·min) was less in VP4 (from 4.3% to 4.4%, p ≤ 0.002) and FLAT (from 2.7% to 3.4%, p ≤ 0.092) vs. OWN across intensities, with a non-significant difference between VP4 and FLAT (1.0%–1.7%, p ≥ 0.292). Findings related to energy cost (W/kg) and energetics cost of transport (J/kg·m) were comparable. VP4 3-km TT performance (11:07.6 ± 0:56.6 mm:ss) was enhanced vs. OWN by 16.6 s (2.4%, p = 0.005) and vs. FLAT by 13.0 s (1.8%, p = 0.032). The 3-km times between OWN and FLAT (0.5%, p = 0.747) were similar. Most runners (n = 11, 61%) ran their fastest TT in VP4.

Conclusion
Overall, VP4 improved laboratory-based RE measures in male recreational runners at relative speeds compared to OWN, but the RE improvements in VP4 were not significant vs. FLAT. More runners exhibited better treadmill TT performances in VP4 (61%) vs. FLAT (22%) and OWN (17%). The variability in RE (–10.3% to 13.3%) and TT (–4.7% to 9.3%) improvements suggests that responses to different types of shoes are individualized and warrant further investigation.

text: Metabolic and performance responses of male runners wearing 3 types of footwear: Nike Vaporfly 4%, Saucony Endorphin racing flats, and their own shoes Purpose
We compared running economy (RE) and 3-km time-trial (TT) variables of runners wearing Nike Vaporfly 4% (VP4), Saucony Endorphin lightweight racing flats (FLAT), and their habitual running (OWN) footwear.

Methods
Eighteen male recreational runners (age = 33.5 ± 11.9 year (mean ± SD), peak oxygen uptake (VO2peak) = 55.8 ± 4.4 mL/kg·min) attended 4 sessions approximately 7 days apart. The first session consisted of a VO2peak test to inform subsequent RE speeds set at 60%, 70%, and 80% of the speed eliciting VO2peak. In subsequent sessions, treadmill RE and 3-km TTs were assessed in the 3 footwear conditions in a randomized, counterbalanced crossover design.

Results
Oxygen consumption (mL/kg·min) was less in VP4 (from 4.3% to 4.4%, p ≤ 0.002) and FLAT (from 2.7% to 3.4%, p ≤ 0.092) vs. OWN across intensities, with a non-significant difference between VP4 and FLAT (1.0%–1.7%, p ≥ 0.292). Findings related to energy cost (W/kg) and energetics cost of transport (J/kg·m) were comparable. VP4 3-km TT performance (11:07.6 ± 0:56.6 mm:ss) was enhanced vs. OWN by 16.6 s (2.4%, p = 0.005) and vs. FLAT by 13.0 s (1.8%, p = 0.032). The 3-km times between OWN and FLAT (0.5%, p = 0.747) were similar. Most runners (n = 11, 61%) ran their fastest TT in VP4.

Conclusion
Overall, VP4 improved laboratory-based RE measures in male recreational runners at relative speeds compared to OWN, but the RE improvements in VP4 were not significant vs. FLAT. More runners exhibited better treadmill TT performances in VP4 (61%) vs. FLAT (22%) and OWN (17%). The variability in RE (–10.3% to 13.3%) and TT (–4.7% to 9.3%) improvements suggests that responses to different types of shoes are individualized and warrant further investigation.

year: 2022

title: How Maori have been affected through the notion and practice of schooling: A theoretical analysis

authors: Williamson, Laurie Thomas

abstract: Education has always been associated according to the public with a necessary function to equip individuals to fit into society when they reach adulthood. The fact that it functions with efficiency is attributed in part to the implementation of social norms, which "impose uniformity of behaviour" (Young 2006, p. 1).

This thesis delves into how Maori have been positioned under a mainstream schooling discourse which has put them at a disadvantage since the implementation of a discourse requires the subjugation of those who are not the majority power holders to assimilate into someone else's discourse. The effect of assimilation into a mainstream discourse has political implications for Maori, as schools are the gateway towards social stratification. This thesis will look at the historical and developmental concept of schooling within a western context, moving towards the development of formal schooling in New Zealand and also look at the sociological and political impact of schooling upon Maori students.

I will also differentiate between the terms schooling and education to suggest Maori forms of educational discourse has been marginalised in favour of assimilation into a supposedly equitable rigid, formal system of schooling. I will illustrate the broader concept of education that takes place outside of schools formal boundaries as a way to contrast the level of control and regulation the government initiates within the formal schooling environment. I will also look at the way schools have been used from its colonial beginnings in New Zealand as a way to civilise or impose one cultural practice on another as a way of breaking down uncivilised beliefs and traditions.

text: How Maori have been affected through the notion and practice of schooling: A theoretical analysis Education has always been associated according to the public with a necessary function to equip individuals to fit into society when they reach adulthood. The fact that it functions with efficiency is attributed in part to the implementation of social norms, which "impose uniformity of behaviour" (Young 2006, p. 1).

This thesis delves into how Maori have been positioned under a mainstream schooling discourse which has put them at a disadvantage since the implementation of a discourse requires the subjugation of those who are not the majority power holders to assimilate into someone else's discourse. The effect of assimilation into a mainstream discourse has political implications for Maori, as schools are the gateway towards social stratification. This thesis will look at the historical and developmental concept of schooling within a western context, moving towards the development of formal schooling in New Zealand and also look at the sociological and political impact of schooling upon Maori students.

I will also differentiate between the terms schooling and education to suggest Maori forms of educational discourse has been marginalised in favour of assimilation into a supposedly equitable rigid, formal system of schooling. I will illustrate the broader concept of education that takes place outside of schools formal boundaries as a way to contrast the level of control and regulation the government initiates within the formal schooling environment. I will also look at the way schools have been used from its colonial beginnings in New Zealand as a way to civilise or impose one cultural practice on another as a way of breaking down uncivilised beliefs and traditions.

year: 2012