title: A theory of change driven approach to evaluating a multi-agency stalking intervention programme

authors: Belur, Jyoti

abstract: Multi agency initiatives as a response to complex crimes and social problems pose several challenges for practitioners and evaluators, in conceptual and operational terms. Challenges for practitioners include working out input requirements to achieve specified interim and final outcomes, and for evaluators it implies measuring not only if interventions work, but how they work, for whom, and under what circumstances. In an attempt to address these challenges, here we present findings from our unique attempt to combine a theory of change driven approach with the realist-inspired EMMIE evaluation framework, of a pilot multi agency stalking intervention partnership in one police force area in England and Wales. The paper makes a significant methodological and empirical contribution to evaluation literature

text: A theory of change driven approach to evaluating a multi-agency stalking intervention programme Multi agency initiatives as a response to complex crimes and social problems pose several challenges for practitioners and evaluators, in conceptual and operational terms. Challenges for practitioners include working out input requirements to achieve specified interim and final outcomes, and for evaluators it implies measuring not only if interventions work, but how they work, for whom, and under what circumstances. In an attempt to address these challenges, here we present findings from our unique attempt to combine a theory of change driven approach with the realist-inspired EMMIE evaluation framework, of a pilot multi agency stalking intervention partnership in one police force area in England and Wales. The paper makes a significant methodological and empirical contribution to evaluation literature

year: 2023-06-30

title: The Structure of the Oligomerization Domain of Lsr2 from Mycobacterium tuberculosis Reveals a Mechanism for Chromosome Organization and Protection

authors: Summers, Emma Louise

subjects: biology

abstract: Lsr2 is a small DNA-binding protein present in mycobacteria and related actinobacteria that regulates gene expression and influences the organization of bacterial chromatin. Lsr2 is a dimer that binds to AT-rich regions of chromosomal DNA and physically protects DNA from damage by reactive oxygen intermediates (ROI). A recent structure of the C-terminal DNA-binding domain of Lsr2 provides a rationale for its interaction with the minor groove of DNA, its preference for AT-rich tracts, and its similarity to other bacterial nucleoid-associated DNA-binding domains. In contrast, the details of Lsr2 dimerization (and oligomerization) via its N-terminal domain, and the mechanism of Lsr2-mediated chromosomal cross-linking and protection is unknown. We have solved the structure of the N-terminal domain of Lsr2 (N-Lsr2) at 1.73 Å resolution using crystallographic ab initio approaches. The structure shows an intimate dimer of two ß-ß-a motifs with no close homologues in the structural databases. The organization of individual N-Lsr2 dimers in the crystal also reveals a mechanism for oligomerization. Proteolytic removal of three N-terminal residues from Lsr2 results in the formation of an anti-parallel β-sheet between neighboring molecules and the formation of linear chains of N-Lsr2. Oligomerization can be artificially induced using low concentrations of trypsin and the arrangement of N-Lsr2 into long chains is observed in both monoclinic and hexagonal crystallographic space groups. In solution, oligomerization of N-Lsr2 is also observed following treatment with trypsin. A change in chromosomal topology after the addition of trypsin to full-length Lsr2-DNA complexes and protection of DNA towards DNAse digestion can be observed using electron microscopy and electrophoresis. These results suggest a mechanism for oligomerization of Lsr2 via protease-activation leading to chromosome compaction and protection, and concomitant down-regulation of large numbers of genes. This mechanism is likely to be relevant under conditions of stress where cellular proteases are known to be upregulated.

text: The Structure of the Oligomerization Domain of Lsr2 from Mycobacterium tuberculosis Reveals a Mechanism for Chromosome Organization and Protection Lsr2 is a small DNA-binding protein present in mycobacteria and related actinobacteria that regulates gene expression and influences the organization of bacterial chromatin. Lsr2 is a dimer that binds to AT-rich regions of chromosomal DNA and physically protects DNA from damage by reactive oxygen intermediates (ROI). A recent structure of the C-terminal DNA-binding domain of Lsr2 provides a rationale for its interaction with the minor groove of DNA, its preference for AT-rich tracts, and its similarity to other bacterial nucleoid-associated DNA-binding domains. In contrast, the details of Lsr2 dimerization (and oligomerization) via its N-terminal domain, and the mechanism of Lsr2-mediated chromosomal cross-linking and protection is unknown. We have solved the structure of the N-terminal domain of Lsr2 (N-Lsr2) at 1.73 Å resolution using crystallographic ab initio approaches. The structure shows an intimate dimer of two ß-ß-a motifs with no close homologues in the structural databases. The organization of individual N-Lsr2 dimers in the crystal also reveals a mechanism for oligomerization. Proteolytic removal of three N-terminal residues from Lsr2 results in the formation of an anti-parallel β-sheet between neighboring molecules and the formation of linear chains of N-Lsr2. Oligomerization can be artificially induced using low concentrations of trypsin and the arrangement of N-Lsr2 into long chains is observed in both monoclinic and hexagonal crystallographic space groups. In solution, oligomerization of N-Lsr2 is also observed following treatment with trypsin. A change in chromosomal topology after the addition of trypsin to full-length Lsr2-DNA complexes and protection of DNA towards DNAse digestion can be observed using electron microscopy and electrophoresis. These results suggest a mechanism for oligomerization of Lsr2 via protease-activation leading to chromosome compaction and protection, and concomitant down-regulation of large numbers of genes. This mechanism is likely to be relevant under conditions of stress where cellular proteases are known to be upregulated.

year: 2012

title: “Landlords wouldn’t give my application a second look.” Discrimination exacerbates inequalities in access to private rental housing

authors: Terruhn, Jessica

abstract: This report and the key insights are based on findings from a housing survey that was conducted with 800 residents in neighbourhoods in Auckland, Hamilton and Christchurch. 
• Perceptions of unfairness in Aotearoa’s housing sector are widespread. Renters, Māori, younger people and low-income groups as well as residents in neighbourhoods with high levels of housing deprivation are most likely to think that people are treated unfairly when trying to rent or buy a home in Aotearoa. 
• Nearly one in three respondents reported having experienced discrimination when trying to rent or buy a home in Aotearoa. Renters, Māori, younger people and low-income groups as well as residents in neighbourhoods with high levels of housing deprivation are most likely to report experiences of discrimination. 
• Advantage and disadvantage in securing a home are determined by a combination of interlocking factors, including income and employment status, age, family status, and race/ethnicity or skin colour. These patterns suggest widespread experiences of potentially unlawful housing discrimination. 
• People strategically try to avoid and mitigate discrimination. Expectations of being discriminated against and of being advantaged influence where and how home seekers search for housing. This finding signals that experiences of rejection play a role in constraining people’s housing choices. 
• The survey findings suggest that discrimination, as part of tenant selection, contributes to housing precarity and inequalities in access to rental housing. Therefore, this research points to an urgent need to address housing discrimination, especially in the context of high levels of residential mobility among renters and intense competition for rental properties.

text: “Landlords wouldn’t give my application a second look.” Discrimination exacerbates inequalities in access to private rental housing This report and the key insights are based on findings from a housing survey that was conducted with 800 residents in neighbourhoods in Auckland, Hamilton and Christchurch. 
• Perceptions of unfairness in Aotearoa’s housing sector are widespread. Renters, Māori, younger people and low-income groups as well as residents in neighbourhoods with high levels of housing deprivation are most likely to think that people are treated unfairly when trying to rent or buy a home in Aotearoa. 
• Nearly one in three respondents reported having experienced discrimination when trying to rent or buy a home in Aotearoa. Renters, Māori, younger people and low-income groups as well as residents in neighbourhoods with high levels of housing deprivation are most likely to report experiences of discrimination. 
• Advantage and disadvantage in securing a home are determined by a combination of interlocking factors, including income and employment status, age, family status, and race/ethnicity or skin colour. These patterns suggest widespread experiences of potentially unlawful housing discrimination. 
• People strategically try to avoid and mitigate discrimination. Expectations of being discriminated against and of being advantaged influence where and how home seekers search for housing. This finding signals that experiences of rejection play a role in constraining people’s housing choices. 
• The survey findings suggest that discrimination, as part of tenant selection, contributes to housing precarity and inequalities in access to rental housing. Therefore, this research points to an urgent need to address housing discrimination, especially in the context of high levels of residential mobility among renters and intense competition for rental properties.

year: 2024-08