title: Caldolase, a chelator-insensitive extracellular serine proteinase from a Thermus spp.

authors: Saravani, G.A.

subjects: biology

abstract: An extracellular alkaline serine proteinase from Thermus strain ToK3 was isolated and purified to homogeneity by (NH4)2SO4 precipitation followed by ion-exchange chromatography on DEAE-cellulose and QAE-Sephadex, affinity chromatography on N alpha-benzyloxycarbonyl-D-phenylalanyl-triethylenetetraminyl-Sepha rose 4B and gel-filtration chromatography on Sephadex G-75. The purified enzyme had a pI of 8.9 and an Mr determined by gel-permeation chromatography of 25,000. The specific activity was about 37,700 proteolytic units/mg with casein as substrate, and the pH optimum was 9.5. Proteolytic activity was inhibited by low concentrations of di-isopropyl phosphorofluoridate and phenylmethanesulphonyl fluoride, but was unaffected by EDTA, EGTA, o-phenanthroline, N-ethyl-5-phenylisoxazolium-3'-sulphonate, N alpha-p-tosyl-L-phenylalanylchloromethane, N alpha-p-tosyl-L-lysylchloromethane, trypsin inhibitors and pepstatin A. The enzyme contained approx. 10% carbohydrate and four disulphide bonds. No Ca2+, Zn2+ or free thiol groups were detected. It hydrolysed several native and dye-linked proteins and synthetic chromogenic peptides and esters. The enzyme was very thermostable (half-life values were 840 min at 80 degrees C, 45 min at 90 degrees C and 5 min at 100 degrees C). The enzyme was unstable at low ionic strength: after 60 min at 75 degrees C in 0.1 M-Tris/acetate buffer, pH 8, only 20% activity remained, compared with no loss in 0.1 M-Tris/acetate buffer, pH 8, containing 0.4 M-NaCl.

text: Caldolase, a chelator-insensitive extracellular serine proteinase from a Thermus spp. An extracellular alkaline serine proteinase from Thermus strain ToK3 was isolated and purified to homogeneity by (NH4)2SO4 precipitation followed by ion-exchange chromatography on DEAE-cellulose and QAE-Sephadex, affinity chromatography on N alpha-benzyloxycarbonyl-D-phenylalanyl-triethylenetetraminyl-Sepha rose 4B and gel-filtration chromatography on Sephadex G-75. The purified enzyme had a pI of 8.9 and an Mr determined by gel-permeation chromatography of 25,000. The specific activity was about 37,700 proteolytic units/mg with casein as substrate, and the pH optimum was 9.5. Proteolytic activity was inhibited by low concentrations of di-isopropyl phosphorofluoridate and phenylmethanesulphonyl fluoride, but was unaffected by EDTA, EGTA, o-phenanthroline, N-ethyl-5-phenylisoxazolium-3'-sulphonate, N alpha-p-tosyl-L-phenylalanylchloromethane, N alpha-p-tosyl-L-lysylchloromethane, trypsin inhibitors and pepstatin A. The enzyme contained approx. 10% carbohydrate and four disulphide bonds. No Ca2+, Zn2+ or free thiol groups were detected. It hydrolysed several native and dye-linked proteins and synthetic chromogenic peptides and esters. The enzyme was very thermostable (half-life values were 840 min at 80 degrees C, 45 min at 90 degrees C and 5 min at 100 degrees C). The enzyme was unstable at low ionic strength: after 60 min at 75 degrees C in 0.1 M-Tris/acetate buffer, pH 8, only 20% activity remained, compared with no loss in 0.1 M-Tris/acetate buffer, pH 8, containing 0.4 M-NaCl.

year: 1989

title: Analysis of Chinese travel blogs of New Zealand

authors: Sun, Minghui

subjects: Content analysis

abstract: The Chinese outbound tourism market is a fast growing source market for many destinations. However, more research is needed to better understand the Chinese outbound tourism market and its diversity and complexity. Again, it can be argued that consistent monitoring of what is a fast evolving market is required, even where prior research has been undertaken. The aim of this research is to provide the tourism industry with a better understanding of the Chinese tourist market and the attitudes of Chinese visitors with specific reference to New Zealand. Consequently perceived destination image, travel motivations, and patterns of online communications of and about New Zealand were identified by analysing travel blogs written by Chinese tourists. Given that there were 181 million bloggers existing in China by the end of 2009 (CNNIC, 2009), blogs written by Chinese visitors have a significant market value for tourism organisations and marketers. 
This study used content analysis to identify the perceived destination image held by Chinese tourists to New Zealand. In this study, the analytical process was divided into two parts, namely photographic and textual analysis. In each of the two parts, qualitative content analysis was first undertaken, and then followed by secondary quantitative analysis to support the findings discerned through the qualitative process. 
By analysing 6968 photographs and 408 blog entries posted by Chinese visitors, an image of New Zealand as a tourism destination was identified. The findings revealed that the main components of New Zealand, as perceived by Chinese visitors, were that it possessed a protected ecological environment, offered a variety of activities, and had a highly developed society with a “Pakeha” culture. The research also indicated that Chinese cultural norms played an important role in the process of decision making, perception formation and interpretation. These norms included the desire of harmony, Li, Junzi Aspiration, and a respect of authorities. The results of the study also included details about travel patterns in dining, accommodation, and tourism activities. These results indicated that content analysis of visual and textual materials provides rich information about perceived destination image, travel preference, and online information search behaviours. Online photographs and narratives posted by visitors are not only valuable information source for potential visitors who read the blogs, but can also be an effective way to understand the Chinese market. 
Based on the results, it can be concluded that blogs are important, and point a way by which destination marketing organisations can seek to influence potential visitors. For example, blog celebrities are an effective way by which to attract thousands of readers to know more about the country. The use of the Chinese language and creating special arrangements for Chinese festivals are shown to be effective means by which hosts can display a friendly attitude to their Chinese guests. With more and more countries being granted Approved Destination Status (ADS) by the Chinese government, Chinese citizens have more choices as to where to travel overseas, thereby intensifying competition for their custom. It is vital for tourism destinations to promote their unique features through all possible channels according to different target markets. Additionally, because service quality is a crucial issue for group tourists, consistent monitoring the travel blogs is an effective way of facilitating the improvement of the service quality of tourism product suppliers.

text: Analysis of Chinese travel blogs of New Zealand The Chinese outbound tourism market is a fast growing source market for many destinations. However, more research is needed to better understand the Chinese outbound tourism market and its diversity and complexity. Again, it can be argued that consistent monitoring of what is a fast evolving market is required, even where prior research has been undertaken. The aim of this research is to provide the tourism industry with a better understanding of the Chinese tourist market and the attitudes of Chinese visitors with specific reference to New Zealand. Consequently perceived destination image, travel motivations, and patterns of online communications of and about New Zealand were identified by analysing travel blogs written by Chinese tourists. Given that there were 181 million bloggers existing in China by the end of 2009 (CNNIC, 2009), blogs written by Chinese visitors have a significant market value for tourism organisations and marketers. 
This study used content analysis to identify the perceived destination image held by Chinese tourists to New Zealand. In this study, the analytical process was divided into two parts, namely photographic and textual analysis. In each of the two parts, qualitative content analysis was first undertaken, and then followed by secondary quantitative analysis to support the findings discerned through the qualitative process. 
By analysing 6968 photographs and 408 blog entries posted by Chinese visitors, an image of New Zealand as a tourism destination was identified. The findings revealed that the main components of New Zealand, as perceived by Chinese visitors, were that it possessed a protected ecological environment, offered a variety of activities, and had a highly developed society with a “Pakeha” culture. The research also indicated that Chinese cultural norms played an important role in the process of decision making, perception formation and interpretation. These norms included the desire of harmony, Li, Junzi Aspiration, and a respect of authorities. The results of the study also included details about travel patterns in dining, accommodation, and tourism activities. These results indicated that content analysis of visual and textual materials provides rich information about perceived destination image, travel preference, and online information search behaviours. Online photographs and narratives posted by visitors are not only valuable information source for potential visitors who read the blogs, but can also be an effective way to understand the Chinese market. 
Based on the results, it can be concluded that blogs are important, and point a way by which destination marketing organisations can seek to influence potential visitors. For example, blog celebrities are an effective way by which to attract thousands of readers to know more about the country. The use of the Chinese language and creating special arrangements for Chinese festivals are shown to be effective means by which hosts can display a friendly attitude to their Chinese guests. With more and more countries being granted Approved Destination Status (ADS) by the Chinese government, Chinese citizens have more choices as to where to travel overseas, thereby intensifying competition for their custom. It is vital for tourism destinations to promote their unique features through all possible channels according to different target markets. Additionally, because service quality is a crucial issue for group tourists, consistent monitoring the travel blogs is an effective way of facilitating the improvement of the service quality of tourism product suppliers.

year: 2013

title: Coastal communities, leisure and wellbeing: Advancing a trans-disciplinary agenda for understanding ocean-human relationships in Aotearoa New Zealand

authors: Wheaton, Belinda

subjects: Science & Technology

abstract: Commentators are advocating for research to better understand relationships between healthy coastal ecosystems and human wellbeing. Doing so requires inter- and transdisciplinary approaches across humanities, arts, social sciences, and science and technology disciplines. These approaches include culturally diverse knowledge systems, such as indigenous ones, that locate sustainable use of and relationships to marine ecosystems. This paper contributes to this agenda through a case-study of relationships between coastal ecosystems and human wellbeing in Aotearoa New Zealand. This article highlights interconnected cultural and wellbeing benefits of, and socio-ecological relationships between, these coastal ecosystems drawing on a case study of one ocean-based, ‘immersive’ leisure activity, surfing. Further, it examines how these relationships impact human physical, emotional and spiritual wellbeing, and the wellbeing of communities and ecosystems. The research illustrates that surfing creates strong bonds between practitioners and coastal places, linking the health of marine environments and people. We demonstrate the value of a transdisciplinary place-based approach that integrates research across the humanities and social sciences and engages with Indigenous knowledge (Mātauranga Māori). This argument for multicultural co-learning shows the value of Western and Māori vantage points for how we understand coastal blue spaces. Indigenous perspectives, we conclude, deepen appreciation, as well as equity considerations, of how we understand place, wellbeing, and long-term sustainable relationships with marine ecosystems.

text: Coastal communities, leisure and wellbeing: Advancing a trans-disciplinary agenda for understanding ocean-human relationships in Aotearoa New Zealand Commentators are advocating for research to better understand relationships between healthy coastal ecosystems and human wellbeing. Doing so requires inter- and transdisciplinary approaches across humanities, arts, social sciences, and science and technology disciplines. These approaches include culturally diverse knowledge systems, such as indigenous ones, that locate sustainable use of and relationships to marine ecosystems. This paper contributes to this agenda through a case-study of relationships between coastal ecosystems and human wellbeing in Aotearoa New Zealand. This article highlights interconnected cultural and wellbeing benefits of, and socio-ecological relationships between, these coastal ecosystems drawing on a case study of one ocean-based, ‘immersive’ leisure activity, surfing. Further, it examines how these relationships impact human physical, emotional and spiritual wellbeing, and the wellbeing of communities and ecosystems. The research illustrates that surfing creates strong bonds between practitioners and coastal places, linking the health of marine environments and people. We demonstrate the value of a transdisciplinary place-based approach that integrates research across the humanities and social sciences and engages with Indigenous knowledge (Mātauranga Māori). This argument for multicultural co-learning shows the value of Western and Māori vantage points for how we understand coastal blue spaces. Indigenous perspectives, we conclude, deepen appreciation, as well as equity considerations, of how we understand place, wellbeing, and long-term sustainable relationships with marine ecosystems.

year: 2021