title: Marketing in the Flourishing Society Megatrend

authors: Varey, Richard J.

subjects: post-industrial business

abstract: “New science” has profound implications for business. Industrial capitalism can no longer power prosperity. The mass society worldview is giving way to individualisation. The “standard enterprise logic” is challenged. Marketing has operated as an attention technology for sellers competing to capture customers. However, in an intention economy buyers are a scarce commodity, and it is intentions that drive production for specific needs. Change in marketing is overdue. Despite increased social disharmony and the mounting evidence of looming environmental disasters, progress is stagnant, often negative, as marketing exacerbates the problem by misallocating negative value goods. The commonality in the contemporary crises of financial meltdown, human-made climate change, economic inequality, distrust of government, and the social corrosion of consumerism is the moral limits of markets in civic society. Sustainable living provides the higher purpose of marketing: well-being and human flourishing. Sustainability is a socio-cultural, inherently ethical, respectful, intellectual construct for a life of careful and equitable resource use within limits and inter-dependencies. It is not the antithesis of competitive business, indeed business can flourish by competing on, and being rewarded for, the accomplishment of enduringly valuable outcomes. Sustainability is a transcendent societal “mega”-megatrend.

text: Marketing in the Flourishing Society Megatrend “New science” has profound implications for business. Industrial capitalism can no longer power prosperity. The mass society worldview is giving way to individualisation. The “standard enterprise logic” is challenged. Marketing has operated as an attention technology for sellers competing to capture customers. However, in an intention economy buyers are a scarce commodity, and it is intentions that drive production for specific needs. Change in marketing is overdue. Despite increased social disharmony and the mounting evidence of looming environmental disasters, progress is stagnant, often negative, as marketing exacerbates the problem by misallocating negative value goods. The commonality in the contemporary crises of financial meltdown, human-made climate change, economic inequality, distrust of government, and the social corrosion of consumerism is the moral limits of markets in civic society. Sustainable living provides the higher purpose of marketing: well-being and human flourishing. Sustainability is a socio-cultural, inherently ethical, respectful, intellectual construct for a life of careful and equitable resource use within limits and inter-dependencies. It is not the antithesis of competitive business, indeed business can flourish by competing on, and being rewarded for, the accomplishment of enduringly valuable outcomes. Sustainability is a transcendent societal “mega”-megatrend.

year: 2013

title: Expression, purification and characterisation of GIGANTEA: A circadian clock-controlled regulator of photoperiodic flowering in plants

authors: Black, Moyra M.

subjects: flowering

abstract: The Arabidopsis thaliana (Arabidopsis) GIGANTEA (GI) gene is a central component of the photoperiodic flowering pathway. While it has been 40 years since the first mutant alleles of GI were described much is still unknown about the molecular mechanism of GI action. To investigate the biochemistry and domain organisation (and ultimately to give a greater understanding of the role of GI in floral induction), it is first necessary to produce significant quantities of purified protein. Soluble affinity-tagged full-length GI was expressed in Escherichia coli (E. coli) and was stabilised by the addition of the detergent n-dodecyl-β-d-maltoside (DDM) to storage and purification buffers. Stabilised GI was purified using a variety of chromatographic methods, and characterised using a selection of biochemical techniques including circular dichroism, and dynamic light scattering. This showed that purified GI contained secondary structure, but was polydisperse in solution. Electron microscopy suggests a possible tetramer arrangement of GI. Limited proteolytic digests and mass spectrometry were used to identify potential GI domains. This led to the identification of a predicted 46 kDa amino-terminal GI domain. GI was also expressed in Sf9 insect cells using the baculovirus expression system. GI produced via this route gave insoluble protein.

text: Expression, purification and characterisation of GIGANTEA: A circadian clock-controlled regulator of photoperiodic flowering in plants The Arabidopsis thaliana (Arabidopsis) GIGANTEA (GI) gene is a central component of the photoperiodic flowering pathway. While it has been 40 years since the first mutant alleles of GI were described much is still unknown about the molecular mechanism of GI action. To investigate the biochemistry and domain organisation (and ultimately to give a greater understanding of the role of GI in floral induction), it is first necessary to produce significant quantities of purified protein. Soluble affinity-tagged full-length GI was expressed in Escherichia coli (E. coli) and was stabilised by the addition of the detergent n-dodecyl-β-d-maltoside (DDM) to storage and purification buffers. Stabilised GI was purified using a variety of chromatographic methods, and characterised using a selection of biochemical techniques including circular dichroism, and dynamic light scattering. This showed that purified GI contained secondary structure, but was polydisperse in solution. Electron microscopy suggests a possible tetramer arrangement of GI. Limited proteolytic digests and mass spectrometry were used to identify potential GI domains. This led to the identification of a predicted 46 kDa amino-terminal GI domain. GI was also expressed in Sf9 insect cells using the baculovirus expression system. GI produced via this route gave insoluble protein.

year: 2010

title: Behind the label: Complexities of identifying Māori whānau in an away from home hospital transfer

authors: Masters-Awatere, Bridgette

subjects: Māori whānau

abstract: This paper highlights two methodological issues that arose for a group of Māori researchers during the first few months of a Māori CoRE funded project. The issues were in relation to defining 1) the evolving construction of Māori whānau in New Zealand, and 2) an away from home hospital transfer. Through the process of reflexive practice about our knowledge constructs the research team highlight some of the complexities that we as indigenous researchers had to navigate when working within a cultural context. We describe the details of the Hospital Transfers project and reflect on the learning experienced during the pilot interview phase of a three year project.

text: Behind the label: Complexities of identifying Māori whānau in an away from home hospital transfer This paper highlights two methodological issues that arose for a group of Māori researchers during the first few months of a Māori CoRE funded project. The issues were in relation to defining 1) the evolving construction of Māori whānau in New Zealand, and 2) an away from home hospital transfer. Through the process of reflexive practice about our knowledge constructs the research team highlight some of the complexities that we as indigenous researchers had to navigate when working within a cultural context. We describe the details of the Hospital Transfers project and reflect on the learning experienced during the pilot interview phase of a three year project.

year: 2017