title: The Analysis of Recreational Drugs in Biological Specimens Using Liquid Chromatography Mass Spectrometry

authors: Lucas, Natasha

subjects: Benzylpiperazine

abstract: In the last few years, the prevalence of legal party pills in New Zealand has risen dramatically. These pills contain new piperazine designer drugs, two of the more common being 1-benzylpiperazine (BZP) and m-trifluoromethylphenylpiperazine (TFMPP). This thesis describes an optimised LC-MS/MS method for the detection of BZP and TFMPP in whole blood, using an automated solid phase extraction (SPE) for sample clean-up. The method was validated on three different days using five replicate samples each day. The standard curve was linear from 7 - 7000 ng/mL for BZP and 10 - 10,000 ng/mL for TFMPP, with coefficients of variation (CV) below 10%, and accuracy greater than 90% for both drugs. The method was used to quantitate samples provided by the Medical Research Institute of New Zealand. Blood levels were used to show concentrations in the blood over time, and relate these to performance of subjects on a driving simulator. The study was stopped after 41% of the participants who received BZP and TFMPP had adverse reactions to the pills, including vomiting and migraines.

The LC-MS/MS method was also used to detect and quantitate methamphetamine, amphetamine, methylenedioxymethamphetamine, methylenedioxyamphetamine, morphine, codeine and 6-monoacetylmorphine in hair. The drugs were extracted from 20 mg of hair using hydrochloric acid in a water bath overnight, then purified using SPE. Validation on three days with five replicate samples gave coefficients of variation (CV) below 12% and acceptable accuracy for all drugs. The method was tested on three samples, previously reported by Environmental Science and Research (ESR) using gas chromatography mass spectrometry (GC-MS) giving results in good agreement.

This thesis describes a sensitive, accurate, reproducible LC-MS/MS method easily adapted to analyse drugs of abuse in different biological matrices. It demonstrates the versatility of LC-MS/MS and its applications in forensic work.

text: The Analysis of Recreational Drugs in Biological Specimens Using Liquid Chromatography Mass Spectrometry In the last few years, the prevalence of legal party pills in New Zealand has risen dramatically. These pills contain new piperazine designer drugs, two of the more common being 1-benzylpiperazine (BZP) and m-trifluoromethylphenylpiperazine (TFMPP). This thesis describes an optimised LC-MS/MS method for the detection of BZP and TFMPP in whole blood, using an automated solid phase extraction (SPE) for sample clean-up. The method was validated on three different days using five replicate samples each day. The standard curve was linear from 7 - 7000 ng/mL for BZP and 10 - 10,000 ng/mL for TFMPP, with coefficients of variation (CV) below 10%, and accuracy greater than 90% for both drugs. The method was used to quantitate samples provided by the Medical Research Institute of New Zealand. Blood levels were used to show concentrations in the blood over time, and relate these to performance of subjects on a driving simulator. The study was stopped after 41% of the participants who received BZP and TFMPP had adverse reactions to the pills, including vomiting and migraines.

The LC-MS/MS method was also used to detect and quantitate methamphetamine, amphetamine, methylenedioxymethamphetamine, methylenedioxyamphetamine, morphine, codeine and 6-monoacetylmorphine in hair. The drugs were extracted from 20 mg of hair using hydrochloric acid in a water bath overnight, then purified using SPE. Validation on three days with five replicate samples gave coefficients of variation (CV) below 12% and acceptable accuracy for all drugs. The method was tested on three samples, previously reported by Environmental Science and Research (ESR) using gas chromatography mass spectrometry (GC-MS) giving results in good agreement.

This thesis describes a sensitive, accurate, reproducible LC-MS/MS method easily adapted to analyse drugs of abuse in different biological matrices. It demonstrates the versatility of LC-MS/MS and its applications in forensic work.

year: 2008

title: Review of Jon and Susan Josephson, “Abductive Inference”

authors: Legg, Catherine

abstract: This article reviews the book '“Abductive Inference” by Jon and Susan Josephson.

text: Review of Jon and Susan Josephson, “Abductive Inference” This article reviews the book '“Abductive Inference” by Jon and Susan Josephson.

year: 1996

title: Dialogue as a method for evolving Mātauranga Māori: Perspectives on the use of embryos in research

authors: Hudson, Maui

subjects: New Zealand

abstract: This paper explores the epistemological divide between mātauranga Māori (Māori knowledge) and science, and considers which cultural concepts have relevance when considering the use of embryos in research. We argue that empowerment is a necessary precursor for a dialogue process to be effective and to maintain the cultural dignity and confidence of the participants. Negotiating spaces to share ideas, concepts and values between different knowledge systems is an important exercise that creates opportunities for innovative thinking. The identification of cultural cues and indigenous reference points for considering how knowledge relating to embryo research might be located within a traditional knowledge schema is a necessary type of activity to ensure mātauranga Māori maintains its usefulness for Māori in a changing society while retaining its indigenous spirit and cultural tradition.

text: Dialogue as a method for evolving Mātauranga Māori: Perspectives on the use of embryos in research This paper explores the epistemological divide between mātauranga Māori (Māori knowledge) and science, and considers which cultural concepts have relevance when considering the use of embryos in research. We argue that empowerment is a necessary precursor for a dialogue process to be effective and to maintain the cultural dignity and confidence of the participants. Negotiating spaces to share ideas, concepts and values between different knowledge systems is an important exercise that creates opportunities for innovative thinking. The identification of cultural cues and indigenous reference points for considering how knowledge relating to embryo research might be located within a traditional knowledge schema is a necessary type of activity to ensure mātauranga Māori maintains its usefulness for Māori in a changing society while retaining its indigenous spirit and cultural tradition.

year: 2010