title: Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discrimination

authors: Smith, Kirsty Fiona

subjects: Cryptic invasion

abstract: Mitochondrial cytochrome oxidase I (COI) gene sequencing (DNA barcoding) of Ciona specimens from New Zealand (NZ) led to the first record of the solitary ascidian Ciona savignyi in the Southern Hemisphere. We sought to quantify C. savignyi COI genetic diversity around the NZ archipelago and to compare this with diversity within C. savignyi's native range in the north-west Pacific. Ciona savignyi specimens were collected from two NZ sites and from three sites around Japan. COI sequences (595 bp) were amplified and measures of genetic diversity were calculated. Based on differences between their COI sequences we developed a PCR-based assay to distinguish C. savignyi from the morphologically similar C. intestinalis. A total of 12 C. savignyi COI haplotypes were recovered from the 76 samples. Of the four haplotypes observed in NZ, two were unique. From the 10 haplotypes observed in the Japan samples, eight were unique. The C. savignyi populations in Japan were found to contain higher haplotype diversity when compared with those in NZ. The NZ samples contained only a small subset of the haplotype variation of the Japan samples, however, NZ samples did harbor two haplotypes not observed in the Japan samples. A PCR-based assay developed from the COI sequences was able to reliably discriminate the two Ciona species. The low COI genetic diversity within the two NZ C. savignyi populations sampled is consistent with a founder effect associated loss of genetic diversity. The robust PCR-based assay for distinguishing C. savignyi and C. intestinalis may find application in ecological and taxonomic studies and can be applied to both archival materials and live animals.

text: Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discrimination Mitochondrial cytochrome oxidase I (COI) gene sequencing (DNA barcoding) of Ciona specimens from New Zealand (NZ) led to the first record of the solitary ascidian Ciona savignyi in the Southern Hemisphere. We sought to quantify C. savignyi COI genetic diversity around the NZ archipelago and to compare this with diversity within C. savignyi's native range in the north-west Pacific. Ciona savignyi specimens were collected from two NZ sites and from three sites around Japan. COI sequences (595 bp) were amplified and measures of genetic diversity were calculated. Based on differences between their COI sequences we developed a PCR-based assay to distinguish C. savignyi from the morphologically similar C. intestinalis. A total of 12 C. savignyi COI haplotypes were recovered from the 76 samples. Of the four haplotypes observed in NZ, two were unique. From the 10 haplotypes observed in the Japan samples, eight were unique. The C. savignyi populations in Japan were found to contain higher haplotype diversity when compared with those in NZ. The NZ samples contained only a small subset of the haplotype variation of the Japan samples, however, NZ samples did harbor two haplotypes not observed in the Japan samples. A PCR-based assay developed from the COI sequences was able to reliably discriminate the two Ciona species. The low COI genetic diversity within the two NZ C. savignyi populations sampled is consistent with a founder effect associated loss of genetic diversity. The robust PCR-based assay for distinguishing C. savignyi and C. intestinalis may find application in ecological and taxonomic studies and can be applied to both archival materials and live animals.

year: 2012

title: Investigating scientific literacy: Scientist’s habits of mind as evidenced by their rationale of science and religious beliefs

authors: Coll, Richard K.

subjects: science

abstract: Science and technology have been incredibly success¬ful in purely technical terms. For instance, international air travel, space flight, and curing of hitherto untreatable medical illnesses all are now routine events. One feature of the incredible (and seemingly ever increasing) advance of science and technology is a sense of unease amongst the general population of science’s potential to change our lives, in sometimes unpredictable and alarming ways. Public understanding of science, or scientific literacy, is of increasing concern worldwide according to much recent literature.

text: Investigating scientific literacy: Scientist’s habits of mind as evidenced by their rationale of science and religious beliefs Science and technology have been incredibly success¬ful in purely technical terms. For instance, international air travel, space flight, and curing of hitherto untreatable medical illnesses all are now routine events. One feature of the incredible (and seemingly ever increasing) advance of science and technology is a sense of unease amongst the general population of science’s potential to change our lives, in sometimes unpredictable and alarming ways. Public understanding of science, or scientific literacy, is of increasing concern worldwide according to much recent literature.

year: 2006

title: Te hau mihi ata mātauranga Māori and science - Cross cultural dialogue

authors: Joseph, Robert

abstract: The Te Hau Mihi Ata Mātauranga Māori and Science research project (the research project) will explore ways to link mātauranga Māori and western science. The purpose of the research project is to open a new creative knowledge space to unleash innovative thinking and cross-cultural connection that is unique to New Zealand which can be a source of untapped economic, social, environmental and cultural potential. The historic marginalisation of alternative value and knowledge systems has prevented quality engagement and dialogue between these two knowledge systems in New Zealand. The research project will develop, inter alia, a new inter-cultural dialogue process between Māori educated in western-based science and experts in mātauranga Māori.

text: Te hau mihi ata mātauranga Māori and science - Cross cultural dialogue The Te Hau Mihi Ata Mātauranga Māori and Science research project (the research project) will explore ways to link mātauranga Māori and western science. The purpose of the research project is to open a new creative knowledge space to unleash innovative thinking and cross-cultural connection that is unique to New Zealand which can be a source of untapped economic, social, environmental and cultural potential. The historic marginalisation of alternative value and knowledge systems has prevented quality engagement and dialogue between these two knowledge systems in New Zealand. The research project will develop, inter alia, a new inter-cultural dialogue process between Māori educated in western-based science and experts in mātauranga Māori.

year: 2008-11