title: Evaluation of lactic acid bacterium from chilli waste as a potential antifungal agent for wood products

authors: O'Callahan, Diahanna Rachelle

subjects: 16S rRNA gene sequencing

abstract: Aims:  The aim of this study was to isolate lactic acid bacteria from chilli waste and evaluate metabolites produced for the ability to arrest wood decay.

Methods and Results:  Using an optical density screening method, one bacterium (isolate C11) was identified as having pronounced antifungal properties against Oligoporus placenta. This isolate was identified as Lactobacillus brevis by 16S rRNA gene sequencing. To determine antifungal activity in wood, Pinus radiata blocks were impregnated with Lact. brevis [C11] cell-free supernatant and exposed to brown rot fungi O. placenta, Antrodia xantha and Coniophora puteana. The treated timber demonstrated resistance to degradation from all fungi. The antifungal metabolites were heat stable and not affected by proteinase K, but were affected by neutralization with NaOH suggesting the metabolites were of an acidic nature. The presence of lactic and acetic acid was confirmed by HPLC analysis.

Conclusions: Lactobacillus brevis [C11] produced acidic metabolites that were able to inhibit the growth of wood decay fungi and subsequent wood decay.

Significance and Impact of the Study:  Traditional wood treatments are becoming an environmental issue as the public demands more benign options. The use of lactic acid bacteria which are considered safe for general use is a potential alternative to the conventional heavy metal chemicals currently in use.

text: Evaluation of lactic acid bacterium from chilli waste as a potential antifungal agent for wood products Aims:  The aim of this study was to isolate lactic acid bacteria from chilli waste and evaluate metabolites produced for the ability to arrest wood decay.

Methods and Results:  Using an optical density screening method, one bacterium (isolate C11) was identified as having pronounced antifungal properties against Oligoporus placenta. This isolate was identified as Lactobacillus brevis by 16S rRNA gene sequencing. To determine antifungal activity in wood, Pinus radiata blocks were impregnated with Lact. brevis [C11] cell-free supernatant and exposed to brown rot fungi O. placenta, Antrodia xantha and Coniophora puteana. The treated timber demonstrated resistance to degradation from all fungi. The antifungal metabolites were heat stable and not affected by proteinase K, but were affected by neutralization with NaOH suggesting the metabolites were of an acidic nature. The presence of lactic and acetic acid was confirmed by HPLC analysis.

Conclusions: Lactobacillus brevis [C11] produced acidic metabolites that were able to inhibit the growth of wood decay fungi and subsequent wood decay.

Significance and Impact of the Study:  Traditional wood treatments are becoming an environmental issue as the public demands more benign options. The use of lactic acid bacteria which are considered safe for general use is a potential alternative to the conventional heavy metal chemicals currently in use.

year: 2011

title: Prototype of a new Engineering Masters project model: Working with marketing and software faculties to commercially kickstart university research

authors: Scott, Jonathan B.

subjects: continuing education

abstract: We describe a Master of Engineering (500-level) project modelled on the real-world arrangement where engineers work with marketing and software groups to prepare a product for commercialisation. A 4-member software team to develop and test embedded firmware and support applications on a mobile platform was provided through a final-year undergraduate software-engineering project course based outside the engineering school, in a separate faculty. A marketing team consisting of interns prepared logos, product names, and advertising materials, with input from a creative 200-level class. This team also considered possible exit strategies based on analysis of the market size and activity. This marketing effort was organised through the management communications group in the management school. The masters student acts as project manager and it is their remit to guide the product towards release on the crowd-sourced venture-capital site kickstarter.com. A small but original product idea is required to provide a viable vehicle for the project. Financial commitment to manufacture, even on a small scale, represents a novel outcome for a university project.

text: Prototype of a new Engineering Masters project model: Working with marketing and software faculties to commercially kickstart university research We describe a Master of Engineering (500-level) project modelled on the real-world arrangement where engineers work with marketing and software groups to prepare a product for commercialisation. A 4-member software team to develop and test embedded firmware and support applications on a mobile platform was provided through a final-year undergraduate software-engineering project course based outside the engineering school, in a separate faculty. A marketing team consisting of interns prepared logos, product names, and advertising materials, with input from a creative 200-level class. This team also considered possible exit strategies based on analysis of the market size and activity. This marketing effort was organised through the management communications group in the management school. The masters student acts as project manager and it is their remit to guide the product towards release on the crowd-sourced venture-capital site kickstarter.com. A small but original product idea is required to provide a viable vehicle for the project. Financial commitment to manufacture, even on a small scale, represents a novel outcome for a university project.

year: 2012

title: Consciousness-centered stewardship: An indigenous standpoint

authors: Spiller, Chellie

abstract: Dominion-driven stewardship, which stages human interventions with an anthropocentric lens, externalises problems. We present consciousness-centered stewardship, seen through the Māori ethic of kaitiakitanga, as the missing connective tissue that looks to fix ourselves. We advocate an approach that appreciates a collective self-intelligence in the world and being a steward with a “conscious mind” as part of a transformative way forward. Sustainable development from this perspective thus includes paying attention to personal growth.

text: Consciousness-centered stewardship: An indigenous standpoint Dominion-driven stewardship, which stages human interventions with an anthropocentric lens, externalises problems. We present consciousness-centered stewardship, seen through the Māori ethic of kaitiakitanga, as the missing connective tissue that looks to fix ourselves. We advocate an approach that appreciates a collective self-intelligence in the world and being a steward with a “conscious mind” as part of a transformative way forward. Sustainable development from this perspective thus includes paying attention to personal growth.

year: 2025