title: Predicting Water Availability in the Antarctic Dry Valleys using Geographic Information Systems and Remote Sensing

authors: Stichbury, Glen

subjects: Antarctica

abstract: Water is one of the most important ingredients for life on Earth. The presence or absence of biologically available water determines whether or not life will exist.  Antarctica is an environment where abiotic constraints, particularly water, strongly influence the distribution and diversity of biota. As Antarctic biology is relatively simple when compared to more temperate climates, it is a prime location for researching constraints on biodiversity, and what may be the impacts of changes to these constraints resulting from climate change and human disturbance. This research uses Geographic Information Systems (GIS) and remote sensing to develop a relative water availability index of three Dry Valleys in Southern Victoria Land, Antarctica. This study area is being used for the IPY Terrestrial Biocomplexity project, an international collaboration researching the distribution, diversity and complexity of biology in the Dry Valleys. The development of a predictive water availability model will contribute greatly to their research goals.
This thesis describes the sources of biologically available water in the Dry Valleys and its interaction with biota. Remotely sensed data of these sources is gathered and various methods of analysing the data are explored. This includes creating a mean snow cover distribution model from MODIS data over 4 summer seasons, and Landsat7 ETM+ surface temperature data. These data sets, combined with a high resolution LIDAR Digital Elevation Model and glacier and lake locations, are then analysed with GIS to produce a Compound Topographic Index (CTI), a model showing the likely accumulation and dispersal of liquid water given the spatial distribution of water sources and the flow of water over the terrain according to the influence of gravity. Visualisation techniques are used to validate the resulting model, including the use of 3D visualisation and comparison of drainage patterns using overlays of a high resolution ALOS image. This research concludes that GIS and remote sensing are valuable tools for predicting water distribution in Antarctica. Although cloud cover, varied illumination and differing spatial resolutions can create limitations, remote sensing's cost effective and environmentally sound method of data capture and the computational and spatial modelling capabilities of GIS make their use well suited to the Antarctic environment.

text: Predicting Water Availability in the Antarctic Dry Valleys using Geographic Information Systems and Remote Sensing Water is one of the most important ingredients for life on Earth. The presence or absence of biologically available water determines whether or not life will exist.  Antarctica is an environment where abiotic constraints, particularly water, strongly influence the distribution and diversity of biota. As Antarctic biology is relatively simple when compared to more temperate climates, it is a prime location for researching constraints on biodiversity, and what may be the impacts of changes to these constraints resulting from climate change and human disturbance. This research uses Geographic Information Systems (GIS) and remote sensing to develop a relative water availability index of three Dry Valleys in Southern Victoria Land, Antarctica. This study area is being used for the IPY Terrestrial Biocomplexity project, an international collaboration researching the distribution, diversity and complexity of biology in the Dry Valleys. The development of a predictive water availability model will contribute greatly to their research goals.
This thesis describes the sources of biologically available water in the Dry Valleys and its interaction with biota. Remotely sensed data of these sources is gathered and various methods of analysing the data are explored. This includes creating a mean snow cover distribution model from MODIS data over 4 summer seasons, and Landsat7 ETM+ surface temperature data. These data sets, combined with a high resolution LIDAR Digital Elevation Model and glacier and lake locations, are then analysed with GIS to produce a Compound Topographic Index (CTI), a model showing the likely accumulation and dispersal of liquid water given the spatial distribution of water sources and the flow of water over the terrain according to the influence of gravity. Visualisation techniques are used to validate the resulting model, including the use of 3D visualisation and comparison of drainage patterns using overlays of a high resolution ALOS image. This research concludes that GIS and remote sensing are valuable tools for predicting water distribution in Antarctica. Although cloud cover, varied illumination and differing spatial resolutions can create limitations, remote sensing's cost effective and environmentally sound method of data capture and the computational and spatial modelling capabilities of GIS make their use well suited to the Antarctic environment.

year: 2009

title: Building script-based tacit knowledge in call centre trainees

authors: McQueen, Robert J.

subjects: tacit knowledge

abstract: An exploratory case study on a technical support call centre in China is presented, which investigates a new paradigm that script-based tacit knowledge for action is built in the brains of learners, rather than transferred. Twelve techniques used by the case study organization to build tacit knowledge scripts in the minds of the trainees are discussed. It was found that one type of script-based tacit knowledge execution can be represented by a set of steps in action-taking that include awareness, attention, diagnosis, action alternative analysis, action taking, and outcome evaluation. The case findings show that the notion of building, rather than transferring tacit knowledge is helpful in understanding the organizational processes used to bring new employees up to working skill levels, and suggest that similar processes and training methods for building tacit knowledge scripts can be used by other organizations to transform a new recruit to an experienced and valued employee.

text: Building script-based tacit knowledge in call centre trainees An exploratory case study on a technical support call centre in China is presented, which investigates a new paradigm that script-based tacit knowledge for action is built in the brains of learners, rather than transferred. Twelve techniques used by the case study organization to build tacit knowledge scripts in the minds of the trainees are discussed. It was found that one type of script-based tacit knowledge execution can be represented by a set of steps in action-taking that include awareness, attention, diagnosis, action alternative analysis, action taking, and outcome evaluation. The case findings show that the notion of building, rather than transferring tacit knowledge is helpful in understanding the organizational processes used to bring new employees up to working skill levels, and suggest that similar processes and training methods for building tacit knowledge scripts can be used by other organizations to transform a new recruit to an experienced and valued employee.

year: 2010

title: Time to Diagnosis and Persistence: The Two Major Determinants of Effective Tuberculosis Control

authors: Ruthe, Alaine Tess

subjects: tuberculosis

abstract: The greatest challenge confronting effective tuberculosis (TB) eradication is the time to diagnosis, and duration of treatment of chronically infected individuals which represent a pool of infection. In an attempt to help limit the spread of TB in New Zealand, a fast SNP based diagnostic test was developed, to quickly identify the highly transmissible and virulent endemic Rangipo strain. The role of VapBC toxin-antitoxin systems in M. tuberculosis has been the subject of great interest recently, due to their expanded number in the genome and links with virulence and the regulation of cell growth in response to environmental stress. Their ability to regulate growth under adverse conditions for presumed survival advantages possibly leading to dormancy or persistence, make them ideal candidates for the development of new M. tuberculosis treatments. To establish differential expression of vapC, and therefore identify possible pathways and functions of the VapBC proteins, RT-qPCR was used to assess the expression levels of vapB and vapC in M. smegmatis under conditions of stress. No consistant changes in vapC mRNA levels were observed, resulting in the hypothesis that it is not the transcriptional differences which are important in the regulation of VapC, but post-transcriptional factors. In order to investigate the function(s) of M. tuberculosis VapBCs, these VapBC proteins were expressed and purified in M. smegmatis, and the VapC toxin tested for RNase activity. The purification, expression, RNase testing and bioinformatic analysis of M. tuberculosis VapCs suggested that VapCRv2530c, VapCRv0065 and VapCRv0617 may all target the same recognition sequence, UA*GG. Bioinformatic analysis revealed an abundance of this target sequence in horizontal gene transfer and TA genes, raising the possibility that VapC toxins could be functioning as selfish elements, or initiating transcriptional regulation cascades when a rapid change in the proteomic response and metabolic state of the cell is required. It is intriguing that the three M. tuberculosis VapC proteins tested thus far appear to target the same recognition sequence, possibly suggesting that all 47 VapCs are RNases and are targeting the same sequence. Alternatively; VapCs may belong to sub-groups targeting different sequences, allowing M. tuberculosis to exude both gross and fine metabolic control; or, they may share the same target, but are regulated by different activators triggered in response to different environmental stimuli.

text: Time to Diagnosis and Persistence: The Two Major Determinants of Effective Tuberculosis Control The greatest challenge confronting effective tuberculosis (TB) eradication is the time to diagnosis, and duration of treatment of chronically infected individuals which represent a pool of infection. In an attempt to help limit the spread of TB in New Zealand, a fast SNP based diagnostic test was developed, to quickly identify the highly transmissible and virulent endemic Rangipo strain. The role of VapBC toxin-antitoxin systems in M. tuberculosis has been the subject of great interest recently, due to their expanded number in the genome and links with virulence and the regulation of cell growth in response to environmental stress. Their ability to regulate growth under adverse conditions for presumed survival advantages possibly leading to dormancy or persistence, make them ideal candidates for the development of new M. tuberculosis treatments. To establish differential expression of vapC, and therefore identify possible pathways and functions of the VapBC proteins, RT-qPCR was used to assess the expression levels of vapB and vapC in M. smegmatis under conditions of stress. No consistant changes in vapC mRNA levels were observed, resulting in the hypothesis that it is not the transcriptional differences which are important in the regulation of VapC, but post-transcriptional factors. In order to investigate the function(s) of M. tuberculosis VapBCs, these VapBC proteins were expressed and purified in M. smegmatis, and the VapC toxin tested for RNase activity. The purification, expression, RNase testing and bioinformatic analysis of M. tuberculosis VapCs suggested that VapCRv2530c, VapCRv0065 and VapCRv0617 may all target the same recognition sequence, UA*GG. Bioinformatic analysis revealed an abundance of this target sequence in horizontal gene transfer and TA genes, raising the possibility that VapC toxins could be functioning as selfish elements, or initiating transcriptional regulation cascades when a rapid change in the proteomic response and metabolic state of the cell is required. It is intriguing that the three M. tuberculosis VapC proteins tested thus far appear to target the same recognition sequence, possibly suggesting that all 47 VapCs are RNases and are targeting the same sequence. Alternatively; VapCs may belong to sub-groups targeting different sequences, allowing M. tuberculosis to exude both gross and fine metabolic control; or, they may share the same target, but are regulated by different activators triggered in response to different environmental stimuli.

year: 2015