title: Acoustic and radio-transmitter retention in common carp (Cyprinus carpio) in New Zealand

authors: Daniel, Adam Joshua

subjects: surgical implant

abstract: Common carp (Cyprinus carpio) are notoriously difficult to monitor in water temperatures above 20◦C using telemetry owing to transmitter expulsion. To reduce transmitter loss, radio transmitters were anchored to the pelvic girdle and a polymer coating was applied to acoustic transmitters to reduce tissue irritation in two tank trials. Ten dummy transmitters were surgically implanted in each of four groups of adult koi carp, a highly coloured strain of C. carpio. Water temperatures ranged between 7 and 24◦C. After 365 days, the control and test groups of each trial had similar expulsion rates (P≥0.30; two-tailed Fisher’s exact probability test). Expulsion rates for uncoated acoustic transmitters were 60% (n=6), coated acoustic transmitters 50% (n=5), unanchored radio transmitters 60% (n=6) and anchored radio transmitters 90% (n=9). Expulsions occurred 15–362 days after implantation as a result of ulcers at or near the surgical wound. Bacterial infection of the wound appears to be the primary mechanism for transmitter expulsion.

text: Acoustic and radio-transmitter retention in common carp (Cyprinus carpio) in New Zealand Common carp (Cyprinus carpio) are notoriously difficult to monitor in water temperatures above 20◦C using telemetry owing to transmitter expulsion. To reduce transmitter loss, radio transmitters were anchored to the pelvic girdle and a polymer coating was applied to acoustic transmitters to reduce tissue irritation in two tank trials. Ten dummy transmitters were surgically implanted in each of four groups of adult koi carp, a highly coloured strain of C. carpio. Water temperatures ranged between 7 and 24◦C. After 365 days, the control and test groups of each trial had similar expulsion rates (P≥0.30; two-tailed Fisher’s exact probability test). Expulsion rates for uncoated acoustic transmitters were 60% (n=6), coated acoustic transmitters 50% (n=5), unanchored radio transmitters 60% (n=6) and anchored radio transmitters 90% (n=9). Expulsions occurred 15–362 days after implantation as a result of ulcers at or near the surgical wound. Bacterial infection of the wound appears to be the primary mechanism for transmitter expulsion.

year: 2009

title: Proteomes and Signalling Pathways of Antler Stem Cells

authors: Li, Chunyi

abstract: As the only known example of complete organ regeneration in mammals, deer antler in the growing or velvet phase is of major interest in developmental biology. This regeneration event initiates from self-renewing antler stem cells that exhibit pluripotency. At present, it remains unclear how the activation and quiescence of antler stem cells are regulated. Therefore, in the present study proteins that were differentially expressed between the antler stem cells and somatic cells (facial periosteum) were identified by a gel-based proteomic technique, and analysed using Ingenuity Pathway Analysis software. Several molecular pathways (PI3K/Akt, ERK/MAPK, p38 MAPK, etc.) were found to be activated during proliferation. Also expressed were the transcription factors POU5F1, SOX2, NANOG and MYC, which are key markers of embryonic stem cells. Expression of these proteins was confirmed in both cultured cells and fresh tissues by Western blot analysis. Therefore, the molecular pathways and transcription factors identified in the current study are common to embryonic and adult stem cells. However, expression of embryonic stem cell transcription factors would suggest that antler stem cells are, potentially, an intermediary stem cell type between embryonic and the more specialized tissue-specific stem cells like those residing in muscle, fat or from a hematopoietic origin. The retention of this embryonic, pluripotent lineage may be of fundamental importance for the subsequent regenerative capacity of antlers.

text: Proteomes and Signalling Pathways of Antler Stem Cells As the only known example of complete organ regeneration in mammals, deer antler in the growing or velvet phase is of major interest in developmental biology. This regeneration event initiates from self-renewing antler stem cells that exhibit pluripotency. At present, it remains unclear how the activation and quiescence of antler stem cells are regulated. Therefore, in the present study proteins that were differentially expressed between the antler stem cells and somatic cells (facial periosteum) were identified by a gel-based proteomic technique, and analysed using Ingenuity Pathway Analysis software. Several molecular pathways (PI3K/Akt, ERK/MAPK, p38 MAPK, etc.) were found to be activated during proliferation. Also expressed were the transcription factors POU5F1, SOX2, NANOG and MYC, which are key markers of embryonic stem cells. Expression of these proteins was confirmed in both cultured cells and fresh tissues by Western blot analysis. Therefore, the molecular pathways and transcription factors identified in the current study are common to embryonic and adult stem cells. However, expression of embryonic stem cell transcription factors would suggest that antler stem cells are, potentially, an intermediary stem cell type between embryonic and the more specialized tissue-specific stem cells like those residing in muscle, fat or from a hematopoietic origin. The retention of this embryonic, pluripotent lineage may be of fundamental importance for the subsequent regenerative capacity of antlers.

year: 2012

title: What a difference a default setting makes

authors: Keegan, Te Taka Adrian Gregory

subjects: computer science

abstract: This paper examines the effect of the default interface language on the usage of a bilingual digital library. In 2005 the default interface language of a bilingual digital library was alternated on a monthly basis between Māori and English. A comprehensive transaction log analysis over this period reveals that not only did usage in a particular language increase when the default interface language was set to that language but that the way the interface was used, in both languages, was quite different depending on the default language.

text: What a difference a default setting makes This paper examines the effect of the default interface language on the usage of a bilingual digital library. In 2005 the default interface language of a bilingual digital library was alternated on a monthly basis between Māori and English. A comprehensive transaction log analysis over this period reveals that not only did usage in a particular language increase when the default interface language was set to that language but that the way the interface was used, in both languages, was quite different depending on the default language.

year: 2008