title: Towards robust and repeatable sampling methods in eDNA-based studies

authors: Dickie, Ian A.

subjects: Science & Technology

abstract: DNA‐based techniques are increasingly used for measuring the biodiversity (species presence, identity, abundance and community composition) of terrestrial and aquatic ecosystems. While there are numerous reviews of molecular methods and bioinformatic steps, there has been little consideration of the methods used to collect samples upon which these later steps are based. This represents a critical knowledge gap, as methodologically sound field sampling is the foundation for subsequent analyses. We reviewed field sampling methods used for metabarcoding studies of both terrestrial and freshwater ecosystem biodiversity over a nearly three‐year period (n = 75). We found that 95% (n = 71) of these studies used subjective sampling methods and inappropriate field methods and/or failed to provide critical methodological information. It would be possible for researchers to replicate only 5% of the metabarcoding studies in our sample, a poorer level of reproducibility than for ecological studies in general. Our findings suggest greater attention to field sampling methods, and reporting is necessary in eDNA‐based studies of biodiversity to ensure robust outcomes and future reproducibility. Methods must be fully and accurately reported, and protocols developed that minimize subjectivity. Standardization of sampling protocols would be one way to help to improve reproducibility and have additional benefits in allowing compilation and comparison of data from across studies.

text: Towards robust and repeatable sampling methods in eDNA-based studies DNA‐based techniques are increasingly used for measuring the biodiversity (species presence, identity, abundance and community composition) of terrestrial and aquatic ecosystems. While there are numerous reviews of molecular methods and bioinformatic steps, there has been little consideration of the methods used to collect samples upon which these later steps are based. This represents a critical knowledge gap, as methodologically sound field sampling is the foundation for subsequent analyses. We reviewed field sampling methods used for metabarcoding studies of both terrestrial and freshwater ecosystem biodiversity over a nearly three‐year period (n = 75). We found that 95% (n = 71) of these studies used subjective sampling methods and inappropriate field methods and/or failed to provide critical methodological information. It would be possible for researchers to replicate only 5% of the metabarcoding studies in our sample, a poorer level of reproducibility than for ecological studies in general. Our findings suggest greater attention to field sampling methods, and reporting is necessary in eDNA‐based studies of biodiversity to ensure robust outcomes and future reproducibility. Methods must be fully and accurately reported, and protocols developed that minimize subjectivity. Standardization of sampling protocols would be one way to help to improve reproducibility and have additional benefits in allowing compilation and comparison of data from across studies.

year: 2018

title: Bounded natural frequencies of completely free rectangular plates

authors: Mochida, Yusuke

abstract: This paper presents dimensionless natural frequency parameters for completely free rectangular plates, calculated using the superposition method (SM) and the finite difference method (FDM). The convergence tests show that while the SM gives upper bound results the FDM gives lower bound results. The results obtained using the FDM appears to be the best lower bound results available to date. Together with the upper bound results from the SM, the maximum possible error in the first 12 non-zero natural frequency parameters for completely free rectangular plates of various aspects ratios ranging from 1:1 to 3:1 can be determined from the results presented here.

text: Bounded natural frequencies of completely free rectangular plates This paper presents dimensionless natural frequency parameters for completely free rectangular plates, calculated using the superposition method (SM) and the finite difference method (FDM). The convergence tests show that while the SM gives upper bound results the FDM gives lower bound results. The results obtained using the FDM appears to be the best lower bound results available to date. Together with the upper bound results from the SM, the maximum possible error in the first 12 non-zero natural frequency parameters for completely free rectangular plates of various aspects ratios ranging from 1:1 to 3:1 can be determined from the results presented here.

year: 2008

title: An exploration of the role of semantic relations in the theory and practice of translation (with special reference to English/Māori and Māori/English translation)

authors: Roa, Tom

subjects: clause relations

abstract: It is over forty years since Beekman and Callow, working within the context of the tagmemic tradition, drew attention to the potential significance so far as the theory and practice of translation are concerned of inter-propositional relations (also referred to as ‘discourse relations’, 'semantic relations', ‘semantico-pragmatic relations’, 'rhetorical relations’ and ‘clause relations’).  Since then, the importance of these relations has been increasingly acknowledged within linguistics and a range of other disciplines, including artificial intelligence. In spite of this, inter-propositional relations remain largely unexplored by the vast majority of those involved in translation. My aim in this research project was to determine (a) what impact, if any, a short training course in inter-propositional relations and inter-propositional relational signalling had on novice Māori-English/ English-Māori translators, and (b)  what impact my own understanding of these relations has had on my own practice as a translator. In connection with this, I conducted two studies.  

In the first of these studies, two small groups of novice translators were asked to translate seven text segments - some from English into te reo Māori, others from te reo Māori into English. They were then given a two-day workshop on inter-propositional relations and inter-propositional relational signalling in English and te reo Māori. No translation exercises were included in the workshop. The novice translators were then asked to translate the same text segments again. Comparison of the two sets of translations revealed some interesting differences, the second set indicating a higher level of sensitivity to inter-propositional relations and their signalling in the original text segments. This resulted in, it is argued here, translations that more accurately reflect authorial intentions as indicated in the source texts (see Chapter 3). In connection with this, it is relevant to note that, as indicated in the introductory chapter (Chapter 1) and in parts of the literature review (Chapter 2), while some of those involved in the Western tradition of translation theory and practice have argued that authorial intention is largely irrelevant in relation to textual interpretation, this essentially post-structural positioning is not available to those involved in translating indigenous texts within the context of indigenous worldviews.

In the second study, I used self-reflective think-aloud protocols to record my thoughts during the process of translating, from English into te reo Māori, text segments from five very different texts, all of these translations having been commissioned by different commissioning agencies. I then, making reference to the think-aloud transcripts, compared the source texts with the translations to determine the ways in which my reflections on inter-propositional relations and their signalling had impacted on the translations (Chapter 4).

My overall conclusion is that an understanding of inter-propositional relations and inter-propositional relational signalling can make a positive contribution to the theory and practice of translation and should be included in translator training and in the teaching of additional languages more generally (Chapter 5).

text: An exploration of the role of semantic relations in the theory and practice of translation (with special reference to English/Māori and Māori/English translation) It is over forty years since Beekman and Callow, working within the context of the tagmemic tradition, drew attention to the potential significance so far as the theory and practice of translation are concerned of inter-propositional relations (also referred to as ‘discourse relations’, 'semantic relations', ‘semantico-pragmatic relations’, 'rhetorical relations’ and ‘clause relations’).  Since then, the importance of these relations has been increasingly acknowledged within linguistics and a range of other disciplines, including artificial intelligence. In spite of this, inter-propositional relations remain largely unexplored by the vast majority of those involved in translation. My aim in this research project was to determine (a) what impact, if any, a short training course in inter-propositional relations and inter-propositional relational signalling had on novice Māori-English/ English-Māori translators, and (b)  what impact my own understanding of these relations has had on my own practice as a translator. In connection with this, I conducted two studies.  

In the first of these studies, two small groups of novice translators were asked to translate seven text segments - some from English into te reo Māori, others from te reo Māori into English. They were then given a two-day workshop on inter-propositional relations and inter-propositional relational signalling in English and te reo Māori. No translation exercises were included in the workshop. The novice translators were then asked to translate the same text segments again. Comparison of the two sets of translations revealed some interesting differences, the second set indicating a higher level of sensitivity to inter-propositional relations and their signalling in the original text segments. This resulted in, it is argued here, translations that more accurately reflect authorial intentions as indicated in the source texts (see Chapter 3). In connection with this, it is relevant to note that, as indicated in the introductory chapter (Chapter 1) and in parts of the literature review (Chapter 2), while some of those involved in the Western tradition of translation theory and practice have argued that authorial intention is largely irrelevant in relation to textual interpretation, this essentially post-structural positioning is not available to those involved in translating indigenous texts within the context of indigenous worldviews.

In the second study, I used self-reflective think-aloud protocols to record my thoughts during the process of translating, from English into te reo Māori, text segments from five very different texts, all of these translations having been commissioned by different commissioning agencies. I then, making reference to the think-aloud transcripts, compared the source texts with the translations to determine the ways in which my reflections on inter-propositional relations and their signalling had impacted on the translations (Chapter 4).

My overall conclusion is that an understanding of inter-propositional relations and inter-propositional relational signalling can make a positive contribution to the theory and practice of translation and should be included in translator training and in the teaching of additional languages more generally (Chapter 5).

year: 2016