title: Rapid purification of two thermophilic proteinases using dye-ligand chromatography

authors: Cowan, Don A.

subjects: thermophilic

abstract: Dye-ligand chromatography has been used successfully for the purification of extracellular thermostable proteinases from thermophilic Bacillus and Thermus cultures. Single step purification factors of up to 115-fold (for Thermus protease) and 2195-fold (for Bacillus protease) were obtained. Elution studies suggested that the mode of binding involved the enzyme active sites. The method was readily scaleable to 600 l volume.

text: Rapid purification of two thermophilic proteinases using dye-ligand chromatography Dye-ligand chromatography has been used successfully for the purification of extracellular thermostable proteinases from thermophilic Bacillus and Thermus cultures. Single step purification factors of up to 115-fold (for Thermus protease) and 2195-fold (for Bacillus protease) were obtained. Elution studies suggested that the mode of binding involved the enzyme active sites. The method was readily scaleable to 600 l volume.

year: 1996

title: Teaching children's literature online: Co-constructing stories in a Massive Open Online Course (MOOC)

authors: Daly, Nicola

abstract: Most scholarship on teaching children’s literature has focused on teaching fiction in university literature courses (Bedford & Albright, 2011; Butler, 2006). While there is a vast literature associated with online teaching dating back more than 20 years (e.g., Palloff & Pratt, 2005), and there is increasing use of online teaching in university contexts (Rapanta et al., 2020), there are very few published descriptions or analyses of the online teaching of children’s literature. In this article we document and discuss the development of a Massive Open Online Course (MOOC) to be delivered in mid-2021 focusing on picturebooks developed at a university, in partnership with a popular MOOC provider. The MOOC development is analysed with respect to supporting the presence of the educators, creating clarity in the delivery of the content, providing spaces for reflection and interaction, and generating human connections in an online environment. These features are linked to the notion of storytelling (Bietti, Tilston & Bangerter, 2019). The contribution of picturebooks to supporting these aspects of effective online teaching is also discussed.

text: Teaching children's literature online: Co-constructing stories in a Massive Open Online Course (MOOC) Most scholarship on teaching children’s literature has focused on teaching fiction in university literature courses (Bedford & Albright, 2011; Butler, 2006). While there is a vast literature associated with online teaching dating back more than 20 years (e.g., Palloff & Pratt, 2005), and there is increasing use of online teaching in university contexts (Rapanta et al., 2020), there are very few published descriptions or analyses of the online teaching of children’s literature. In this article we document and discuss the development of a Massive Open Online Course (MOOC) to be delivered in mid-2021 focusing on picturebooks developed at a university, in partnership with a popular MOOC provider. The MOOC development is analysed with respect to supporting the presence of the educators, creating clarity in the delivery of the content, providing spaces for reflection and interaction, and generating human connections in an online environment. These features are linked to the notion of storytelling (Bietti, Tilston & Bangerter, 2019). The contribution of picturebooks to supporting these aspects of effective online teaching is also discussed.

year: 2021

title: Kaumatua Mana Motuhake: peer education intervention to help Maori elders during later-stage life transitions

authors: Oetzel, John G.

subjects: Science & Technology

abstract: Background
Aotearoa/New Zealand has a population that is ageing and there are challenges to health and social outcomes related to related to key life transitions (e.g., retirement, change in health conditions, loss of spouse). Further, there are significant inequities between Māori (Indigenous people) and non-Māori in ageing outcomes. The purpose of this study was to test the impacts and cost effectiveness of a tuakana/teina (peer education) intervention on kaumātua (elders) receiving the intervention. This study was framed by a strengths-based approach based on the key cultural concept of mana motuhake (autonomy and self-actualisation).

Methods
This study was grounded in principles of Kaupapa Māori and community-based participatory research to bring together a diverse group of stakeholders to co-develop and co-evaluate the intervention. The intervention had tuakana (peer educators) having conversations with up to six teina (recipients) and providing information related to health and social services. The research design was a pre- and post-test, clustered staggered design. Participants completed a baseline assessment of health and mana motuhake measures consistent with Māori worldviews along with two follow-up assessments (one after the first intervention group completed its activities and a second after the second intervention group completed its activities). Additionally, five focus groups and open-ended questions on the assessments were used to provide qualitative evaluation.

Findings
A total of 180 kaumātua were recruited to the intervention with 121 completing it. The analysis revealed improvements over time in the expected direction on most of the variables. However, only three of the variables had statistically significant intervention effects: received support, tribal identity, and trouble paying bills. Qualitative results supported impacts of the intervention on mana motuhake, social connectedness, and tangible/information support related to services. Cost-effectiveness analysis showed that the intervention is cost effective, with a cost per QALY of less than the conventional threshold of three times gross domestic product per capita.

Conclusions
The findings support the relevancy and importance of kaumātua knowledge to create a strengths-based approach to improve health and social outcomes. This study demonstrates that a contextually based and culturally safe age-friendly environments can facilitate engagement and participation by kaumātua for kaumātua.

Trial registry
Australia New Zealand Clinical Trial Registry (ACTRN12617001396314); Date Registered: 3 October 2017 (retrospectively registered); https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=373733&isClinicalTrial=False

text: Kaumatua Mana Motuhake: peer education intervention to help Maori elders during later-stage life transitions Background
Aotearoa/New Zealand has a population that is ageing and there are challenges to health and social outcomes related to related to key life transitions (e.g., retirement, change in health conditions, loss of spouse). Further, there are significant inequities between Māori (Indigenous people) and non-Māori in ageing outcomes. The purpose of this study was to test the impacts and cost effectiveness of a tuakana/teina (peer education) intervention on kaumātua (elders) receiving the intervention. This study was framed by a strengths-based approach based on the key cultural concept of mana motuhake (autonomy and self-actualisation).

Methods
This study was grounded in principles of Kaupapa Māori and community-based participatory research to bring together a diverse group of stakeholders to co-develop and co-evaluate the intervention. The intervention had tuakana (peer educators) having conversations with up to six teina (recipients) and providing information related to health and social services. The research design was a pre- and post-test, clustered staggered design. Participants completed a baseline assessment of health and mana motuhake measures consistent with Māori worldviews along with two follow-up assessments (one after the first intervention group completed its activities and a second after the second intervention group completed its activities). Additionally, five focus groups and open-ended questions on the assessments were used to provide qualitative evaluation.

Findings
A total of 180 kaumātua were recruited to the intervention with 121 completing it. The analysis revealed improvements over time in the expected direction on most of the variables. However, only three of the variables had statistically significant intervention effects: received support, tribal identity, and trouble paying bills. Qualitative results supported impacts of the intervention on mana motuhake, social connectedness, and tangible/information support related to services. Cost-effectiveness analysis showed that the intervention is cost effective, with a cost per QALY of less than the conventional threshold of three times gross domestic product per capita.

Conclusions
The findings support the relevancy and importance of kaumātua knowledge to create a strengths-based approach to improve health and social outcomes. This study demonstrates that a contextually based and culturally safe age-friendly environments can facilitate engagement and participation by kaumātua for kaumātua.

Trial registry
Australia New Zealand Clinical Trial Registry (ACTRN12617001396314); Date Registered: 3 October 2017 (retrospectively registered); https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=373733&isClinicalTrial=False

year: 2020