title: Tetramethyl-p-phenylenediamine (TMPD) oxidase activity and cytochrome distribution in the genus

authors: West, P.A.

subjects: biology

abstract: Kovacs' [1] qualitative test for N,N,N',N',-tetramethyl- p-phenylenediamine (TMPD) oxidase activity is a fundamental test in the identification of bacteria [2,3]. TMPD (E~ = +0.26 V at pH 7.0) acts as an artificial electron donor to the terminal portion of aerobic respiratory systems [4], and the ability to oxidise TMPD, reduced by ascorbate, has been widely used as a quantitative assay of terminal oxidase activity [5]. In a wide range of heterotrophic bacteria there is a close correlation between their response to the Kovacs test and their ascorbate-TMPD oxidase activity measured manometrically [6]. The inability to oxidise ascorbate-TMPD or a negative response to the Kovacs test, however, does not necessarily indicate that an organism lacks an aerobic respiratory system and a terminal oxidase. For example, species belonging to the family Enterobacteriaceae are oxidase- negative yet form aerobic respiratory systems[5,7].

text: Tetramethyl-p-phenylenediamine (TMPD) oxidase activity and cytochrome distribution in the genus Kovacs' [1] qualitative test for N,N,N',N',-tetramethyl- p-phenylenediamine (TMPD) oxidase activity is a fundamental test in the identification of bacteria [2,3]. TMPD (E~ = +0.26 V at pH 7.0) acts as an artificial electron donor to the terminal portion of aerobic respiratory systems [4], and the ability to oxidise TMPD, reduced by ascorbate, has been widely used as a quantitative assay of terminal oxidase activity [5]. In a wide range of heterotrophic bacteria there is a close correlation between their response to the Kovacs test and their ascorbate-TMPD oxidase activity measured manometrically [6]. The inability to oxidise ascorbate-TMPD or a negative response to the Kovacs test, however, does not necessarily indicate that an organism lacks an aerobic respiratory system and a terminal oxidase. For example, species belonging to the family Enterobacteriaceae are oxidase- negative yet form aerobic respiratory systems[5,7].

year: 1978

title: Modelling sustainable ecotourism development on the Coromandel Peninsula in Aotearoa / New Zealand; a holistic systems approach based on the idea of chaos and complexity in a human-activity system

authors: Holzapfel, Rupert

abstract: This thesis studies ecotourism in the context of sustainable tourism development. The research is based on the premise that ecotourism and sustainable development can be expressed as operational theoretical concepts and as fields of empirical inquiry. Positioned in the realm of applied qualitative research in the social sciences, the study’s leitmotiv is that sustainable ecotourism development can be represented from an integrative perspective by designing a conceptual system model. The field work consists of an empirical inquiry placed in the Coromandel Peninsula in New Zealand/Aotearoa. By employing a regional case study to test the hypotheses of the thesis, the research attains an insight in the operationalisation of ecotourism and sustainable ecotourism development. It further produces new knowledge regarding the theorisation and conceptualisation of ecotourism and sustainable tourism development. 

Two main goals drive the study. The first is the exploration of the ontological, epistemological and ideological matrix of a holistic and systemic research perspective. The second goal is the examination of the methodological and practical utility of conceptual system modelling as a research approach. The adopted strategy allows for causal, correlative and teleological interpretations of the spatio-temporal physical and mental phenomena encountered.

With reference to critical realism the modelling process is recognised as an abstraction of ‘actual reality’ as opposed to ‘real reality’. Critical realism as an ontology accounts for the different ‘situated knowledges’ and worldviews that are present in the Coromandel Peninsula. The model itself reflects the researcher’s perception of an ‘empirical reality’, which is depicted at three resolution levels. Progressively coupling the different scales, the model design focuses on: (1) The configuration and behavioural patterns of the system as a whole; (2) the attributes of nested subsystems and their influences on each other as well as on the whole system; (3) the properties of individual system constituents, the processes and relationships linking these elements, and their effects on subsets of the system as well as on the system as a whole. Structural and process analyses, as well as an aetiological account of the system’s variables, do justice to the experienced complexity.

At each resolution level the research outcome entails two simultaneously developed models. Both show the characteristics of open, complex and adaptive human-activity systems. While the first model reflects the status quo of sustainable ecotourism development in the Coromandel Peninsula, the second one represents an idealised archetype that can be used as a grid for further improvements. Neither model offers a fait accompli. Having identified ecotourism and sustainable tourism development as subjective and dynamic problem areas, answers exist within a continuum of differential interpretations, satisfying changing interests, needs and expectations. Solutions are thus of a suggestive and tentative nature. 
On a theoretical level, the study utilises ideas derived from ‘general system theory’ and the ‘chaoplexity paradigm’. Conceptually, it expands the philosophical notion of methodological holism into a pluralistic approach. Methodological triangulation is employed to compensate for the anticipated shortcomings of individual methods. In a pragmatic sense ecotourism and sustainable tourism development are viewed as anthropogenic phenomena that emerge at the interface between humans and the natural environment. Human agency is interpreted as the fulcrum of the system’s evolution, which operates in both the mental and physical dimension.

Assuming that humans possess ‘free will’, and that rational and irrational as well as emotive and intuitive behaviour are inherent faculties of our nature, the system’s dynamics can not be sufficiently described via linear causalities. Non-linear relations, and a complex combination of multivariate and contingent causation, are interpreted predominantly as a result of human encounter and interaction. Answers to what should be ‘right’ and ‘wrong’ in ecotourism practice are based on the adoption of a pluralistic moral stance. This approach allows for competitive as well as cooperative elements as inherent human character traits that drive decision-making processes.

Based on the findings, the thesis concludes with a flexible template of systemic indices that can evaluate the environmental performance and development of ecotourism. It is argued that utilising the suggested set of complex indicators in conjunction bears the potential to enhance sustainable ecotourism development. The template’s adaptability to specific situational contexts is viewed as a prerequisite to cater for changing demands and expectations of individuals, local communities and regions.

text: Modelling sustainable ecotourism development on the Coromandel Peninsula in Aotearoa / New Zealand; a holistic systems approach based on the idea of chaos and complexity in a human-activity system This thesis studies ecotourism in the context of sustainable tourism development. The research is based on the premise that ecotourism and sustainable development can be expressed as operational theoretical concepts and as fields of empirical inquiry. Positioned in the realm of applied qualitative research in the social sciences, the study’s leitmotiv is that sustainable ecotourism development can be represented from an integrative perspective by designing a conceptual system model. The field work consists of an empirical inquiry placed in the Coromandel Peninsula in New Zealand/Aotearoa. By employing a regional case study to test the hypotheses of the thesis, the research attains an insight in the operationalisation of ecotourism and sustainable ecotourism development. It further produces new knowledge regarding the theorisation and conceptualisation of ecotourism and sustainable tourism development. 

Two main goals drive the study. The first is the exploration of the ontological, epistemological and ideological matrix of a holistic and systemic research perspective. The second goal is the examination of the methodological and practical utility of conceptual system modelling as a research approach. The adopted strategy allows for causal, correlative and teleological interpretations of the spatio-temporal physical and mental phenomena encountered.

With reference to critical realism the modelling process is recognised as an abstraction of ‘actual reality’ as opposed to ‘real reality’. Critical realism as an ontology accounts for the different ‘situated knowledges’ and worldviews that are present in the Coromandel Peninsula. The model itself reflects the researcher’s perception of an ‘empirical reality’, which is depicted at three resolution levels. Progressively coupling the different scales, the model design focuses on: (1) The configuration and behavioural patterns of the system as a whole; (2) the attributes of nested subsystems and their influences on each other as well as on the whole system; (3) the properties of individual system constituents, the processes and relationships linking these elements, and their effects on subsets of the system as well as on the system as a whole. Structural and process analyses, as well as an aetiological account of the system’s variables, do justice to the experienced complexity.

At each resolution level the research outcome entails two simultaneously developed models. Both show the characteristics of open, complex and adaptive human-activity systems. While the first model reflects the status quo of sustainable ecotourism development in the Coromandel Peninsula, the second one represents an idealised archetype that can be used as a grid for further improvements. Neither model offers a fait accompli. Having identified ecotourism and sustainable tourism development as subjective and dynamic problem areas, answers exist within a continuum of differential interpretations, satisfying changing interests, needs and expectations. Solutions are thus of a suggestive and tentative nature. 
On a theoretical level, the study utilises ideas derived from ‘general system theory’ and the ‘chaoplexity paradigm’. Conceptually, it expands the philosophical notion of methodological holism into a pluralistic approach. Methodological triangulation is employed to compensate for the anticipated shortcomings of individual methods. In a pragmatic sense ecotourism and sustainable tourism development are viewed as anthropogenic phenomena that emerge at the interface between humans and the natural environment. Human agency is interpreted as the fulcrum of the system’s evolution, which operates in both the mental and physical dimension.

Assuming that humans possess ‘free will’, and that rational and irrational as well as emotive and intuitive behaviour are inherent faculties of our nature, the system’s dynamics can not be sufficiently described via linear causalities. Non-linear relations, and a complex combination of multivariate and contingent causation, are interpreted predominantly as a result of human encounter and interaction. Answers to what should be ‘right’ and ‘wrong’ in ecotourism practice are based on the adoption of a pluralistic moral stance. This approach allows for competitive as well as cooperative elements as inherent human character traits that drive decision-making processes.

Based on the findings, the thesis concludes with a flexible template of systemic indices that can evaluate the environmental performance and development of ecotourism. It is argued that utilising the suggested set of complex indicators in conjunction bears the potential to enhance sustainable ecotourism development. The template’s adaptability to specific situational contexts is viewed as a prerequisite to cater for changing demands and expectations of individuals, local communities and regions.

year: 2003

title: The New Zealand Wars Documentary Series: Discursive Struggle and Cultural Memory.

authors: Perrott, Lisa

subjects: Television Documentary

abstract: The 1998 television broadcast of The New Zealand Wars documentary series was a significant public event, which had a major impact on a broad range of communities and individuals in Aotearoa New Zealand. This popular television history engaged with issues of historical veracity, race, culture and nationhood and challenged previously dominant discourses associated with these concepts. In doing so, it provoked heated debate, and a re-imagining of 'nation', and also opened up spaces for alternative ways of engaging with historical narrative. 

Informed by post-colonialism, cultural studies and cultural memory, this thesis explores the discursive and affective role of The New Zealand Wars, as it has operated within the turbulent climate of 1990s New Zealand cultural relations. This catalytic function is described in this thesis as a phenomenon of a television series shaped by, whilst also intervening in, processes of cultural colonisation and decolonisation. 

While both of these processes involve the transmission of discourse via cultural forms, the act of cultural decolonisation requires, in addition, the convergence of a number of agents (people and communities, discursive and memory resources) and circumstances, within particular contextual conditions. Such a convergence provided the conditions for the discursive synthesis, which shaped the production, construction and reception of this series. 

The role of audio-visual media (and specifically television documentary) in transmitting cultural memory is significant as it enables the flow of memory through channels or forms (such as visual, oral and aural traditions) that can bring about new perspectives and critical reflections upon colonial discourse and dominant concepts of nation and culture. In addition to these social and intellectual processes of audience engagement, this thesis argues that experiential and affective dimensions of cultural memory can (in these specific circumstances) open up radical spaces, offering the potential for generating awareness and sparking political action. 

These issues are explored through a tripartite analysis of the production context, construction and reception of The New Zealand Wars series. The integration of these three phases of analysis has generated a number of insights into the potential of audio-visual forms, including their producers and audiences, to participate in the negotiation of, and resistance to, colonial discourse. Such insights serve to challenge taken-for-granted constructions of nation and history, and suggest the increasing relevance of alternative concepts such as community-building and cultural memory. Ultimately, this thesis argues that television documentary can serve as a prime site for the articulation of these concepts. The New Zealand Wars serves as a case study, which demonstrates both the potential of this site, and the significance of the social-historical and cultural context in framing this series.

text: The New Zealand Wars Documentary Series: Discursive Struggle and Cultural Memory. The 1998 television broadcast of The New Zealand Wars documentary series was a significant public event, which had a major impact on a broad range of communities and individuals in Aotearoa New Zealand. This popular television history engaged with issues of historical veracity, race, culture and nationhood and challenged previously dominant discourses associated with these concepts. In doing so, it provoked heated debate, and a re-imagining of 'nation', and also opened up spaces for alternative ways of engaging with historical narrative. 

Informed by post-colonialism, cultural studies and cultural memory, this thesis explores the discursive and affective role of The New Zealand Wars, as it has operated within the turbulent climate of 1990s New Zealand cultural relations. This catalytic function is described in this thesis as a phenomenon of a television series shaped by, whilst also intervening in, processes of cultural colonisation and decolonisation. 

While both of these processes involve the transmission of discourse via cultural forms, the act of cultural decolonisation requires, in addition, the convergence of a number of agents (people and communities, discursive and memory resources) and circumstances, within particular contextual conditions. Such a convergence provided the conditions for the discursive synthesis, which shaped the production, construction and reception of this series. 

The role of audio-visual media (and specifically television documentary) in transmitting cultural memory is significant as it enables the flow of memory through channels or forms (such as visual, oral and aural traditions) that can bring about new perspectives and critical reflections upon colonial discourse and dominant concepts of nation and culture. In addition to these social and intellectual processes of audience engagement, this thesis argues that experiential and affective dimensions of cultural memory can (in these specific circumstances) open up radical spaces, offering the potential for generating awareness and sparking political action. 

These issues are explored through a tripartite analysis of the production context, construction and reception of The New Zealand Wars series. The integration of these three phases of analysis has generated a number of insights into the potential of audio-visual forms, including their producers and audiences, to participate in the negotiation of, and resistance to, colonial discourse. Such insights serve to challenge taken-for-granted constructions of nation and history, and suggest the increasing relevance of alternative concepts such as community-building and cultural memory. Ultimately, this thesis argues that television documentary can serve as a prime site for the articulation of these concepts. The New Zealand Wars serves as a case study, which demonstrates both the potential of this site, and the significance of the social-historical and cultural context in framing this series.

year: 2007