title: Characterisation of four strains of thermophilic spirochetes and characterisation of phosphofructokinase from isolate Rt 118.B2

authors: Apimerika, Lisa

subjects: Spirochetes

abstract: Four strains of spirochetes were purified by the selective use of antibiotics. Microscopy revealed the strains have typical spirochete morphology. The strains were obligate anaerobes and all four strains utilised a variety of carbohydrates including polysaccharides -but not amino acids- as energy sources. The optimum pH of the isolates was between 6.5 and 7.0. The cardinal growth temperatures varied between the different strains from 52°C to 59°C. The lowest temperature any of the four strains could grow at was 37°C and the highest was 62°C. Doubling times of the isolates varied from 221 minutes to 333 minutes. The major products of glucose fermentations were acetate and lactic acid, no ethanol was formed. All strains were resistant to rifampicin, naldixic acid, novobiocin and polymyxin B. Determination of the G+C mol% of DNA revealed that the strains have the lowest G+C % of the species. 
Cell free extracts from the isolates were assayed for pyrophosphate dependent phosphofructokinase (PFK) activity and the results were compared with those from other members of the species. Attempts were made to purify PFK from one of the isolates. The partially purified enzyme was characterised and was found to have an optimum pH of 7 and optimum temperature of 60°C. The thermostability of the enzyme at various temperatures was established. The kinetics of the enzyme were examined and the Kₘ of fructose-6-phosphate, pyrophosphate and tripolyphosphate established. Various polyphosphates were used as phosphoryl donors; this is the first report of polyphosphates being used as a phosphoryl donor for PFK.

text: Characterisation of four strains of thermophilic spirochetes and characterisation of phosphofructokinase from isolate Rt 118.B2 Four strains of spirochetes were purified by the selective use of antibiotics. Microscopy revealed the strains have typical spirochete morphology. The strains were obligate anaerobes and all four strains utilised a variety of carbohydrates including polysaccharides -but not amino acids- as energy sources. The optimum pH of the isolates was between 6.5 and 7.0. The cardinal growth temperatures varied between the different strains from 52°C to 59°C. The lowest temperature any of the four strains could grow at was 37°C and the highest was 62°C. Doubling times of the isolates varied from 221 minutes to 333 minutes. The major products of glucose fermentations were acetate and lactic acid, no ethanol was formed. All strains were resistant to rifampicin, naldixic acid, novobiocin and polymyxin B. Determination of the G+C mol% of DNA revealed that the strains have the lowest G+C % of the species. 
Cell free extracts from the isolates were assayed for pyrophosphate dependent phosphofructokinase (PFK) activity and the results were compared with those from other members of the species. Attempts were made to purify PFK from one of the isolates. The partially purified enzyme was characterised and was found to have an optimum pH of 7 and optimum temperature of 60°C. The thermostability of the enzyme at various temperatures was established. The kinetics of the enzyme were examined and the Kₘ of fructose-6-phosphate, pyrophosphate and tripolyphosphate established. Various polyphosphates were used as phosphoryl donors; this is the first report of polyphosphates being used as a phosphoryl donor for PFK.

year: 1996

title: Predicting regression test failures using genetic algorithm-selected dynamic performance analysis metrics

authors: Mayo, Michael

subjects: computer science

abstract: A novel framework for predicting regression test failures is proposed. The basic principle embodied in the framework is to use performance analysis tools to capture the runtime behaviour of a program as it executes each test in a regression suite. The performance information is then used to build a dynamically predictive model of test outcomes. Our framework is evaluated using a genetic algorithm for dynamic metric selection in combination with state-of-the-art machine learning classifiers. We show that if a program is modified and some tests subsequently fail, then it is possible to predict with considerable accuracy which of the remaining tests will also fail which can be used to help prioritise tests in time constrained testing environments.

text: Predicting regression test failures using genetic algorithm-selected dynamic performance analysis metrics A novel framework for predicting regression test failures is proposed. The basic principle embodied in the framework is to use performance analysis tools to capture the runtime behaviour of a program as it executes each test in a regression suite. The performance information is then used to build a dynamically predictive model of test outcomes. Our framework is evaluated using a genetic algorithm for dynamic metric selection in combination with state-of-the-art machine learning classifiers. We show that if a program is modified and some tests subsequently fail, then it is possible to predict with considerable accuracy which of the remaining tests will also fail which can be used to help prioritise tests in time constrained testing environments.

year: 2013

title: He whakaemi kōrero mō Ngā Ruahine

authors: Manuirirangi, Hōri

abstract: Ko te whāinga o tēnei tuhingaroa, hei whakaemi kōrero mō Ngā Ruahine. I whāia e au te āhua o te tipu o te rākau hei hanga i aku kōhinga kōrero mō Ngā Ruahine. Ko te ūpoko tuatahi, ko ngā pakiaka, arā te tīmatatanga o Ngā Ruahine. I konei ka tirohia ngā kōrero mō ngā waka tawhito mete whakapapa hoki o ngā tūpuna tāngatawhenua o Ngā Ruahine. Whai muri i tēnā, he wāhanga e pā ana ki te haeretanga mai o Aotea waka, ā, ka tirohia hoki tētehi kōrero tawhito mō te wehenga o ngā iwi o Aotea.
Mai i ngā pakiaka ka piki ake ki te tinana o te rākau. Koinei te wāhanga e pā ana ki ngā korero whenua o Ngā Ruahine. Kei te ūpoko tuarua nei, ka āta tirohia ngā kōrero mō ngā tongi o te rohe o Ngā Ruahine, me ngā take kōrero e whakawhanaunga ana i te tangata ki te whenua. Kei te ūpoko tuarua anō hoki ētehi whakaaturanga whenua mō te rohe o Ngā Ruahine, hei āwhina i te kaipānui.  
Mai i te tinana, ka aro atu ki ngā peka o te rākau i raro i te ūpoko tuatoru. Koinei te wāhanga nui o tēnei tuhingaroa, nā te tokomaha o ngā toronga o ngā peka nei. I roto i te ūpoko tuatoru, ka tirohia ētehi kōrero mō ia hapū o Ngā Ruahine. Mai i ngā kōrero mō ngā rohe whenua o ia hapū, ki ngā kōrero mō ngā marae me ngā pā tawhito o ērā hapū, ā, tae atu hoki ki ngā tūpuna rongonui me ā rātou mahi whakaari.  
Hei te mutunga, ka herea katoatia ngā kōrero o te tuhinga nei i raro i te ūpoko tuawhā, kia kite ai , he aha ngā hua e pūawai ai i tēnei tuhinga. Koinei te whakamutunga o ngā kōrero, arā ko te whakakapīnga o ngā kōrero.  
Ko tēnei tuhingaroa, he whakaemi kōrero mai i ngā kaumātua o Ngā Ruahine, ā, he mea tango mai hoki ētehi kōrero i ngā pukapuka, ko ēnei kōrero kua whakatakotoria i roto i tēnei tuhingaroa hei whakawhāriki iho ki te aroaro o te tini. Ko te tūmanako, he tuhinga rangahau tēnei hei āwhina i ngā rangatahi o Ngā Ruahine, kia rongo ai rātou i ngā kōrero kāore i te tino rangona i ēnei rā, kia mau hoki ngā kōrero a ngā kaumātua o Ngā Ruahine, kei ngaro. Erangi, he whakamōhio tēnei, he tīmatanga anahe tēnei tuhingaroa mō te kaupapa nei. Ko te tūmanako, he hua anō ka puta i tēnei rangahau, hei whāngai i ngā manu hiakai o Ngā Ruahine.

text: He whakaemi kōrero mō Ngā Ruahine Ko te whāinga o tēnei tuhingaroa, hei whakaemi kōrero mō Ngā Ruahine. I whāia e au te āhua o te tipu o te rākau hei hanga i aku kōhinga kōrero mō Ngā Ruahine. Ko te ūpoko tuatahi, ko ngā pakiaka, arā te tīmatatanga o Ngā Ruahine. I konei ka tirohia ngā kōrero mō ngā waka tawhito mete whakapapa hoki o ngā tūpuna tāngatawhenua o Ngā Ruahine. Whai muri i tēnā, he wāhanga e pā ana ki te haeretanga mai o Aotea waka, ā, ka tirohia hoki tētehi kōrero tawhito mō te wehenga o ngā iwi o Aotea.
Mai i ngā pakiaka ka piki ake ki te tinana o te rākau. Koinei te wāhanga e pā ana ki ngā korero whenua o Ngā Ruahine. Kei te ūpoko tuarua nei, ka āta tirohia ngā kōrero mō ngā tongi o te rohe o Ngā Ruahine, me ngā take kōrero e whakawhanaunga ana i te tangata ki te whenua. Kei te ūpoko tuarua anō hoki ētehi whakaaturanga whenua mō te rohe o Ngā Ruahine, hei āwhina i te kaipānui.  
Mai i te tinana, ka aro atu ki ngā peka o te rākau i raro i te ūpoko tuatoru. Koinei te wāhanga nui o tēnei tuhingaroa, nā te tokomaha o ngā toronga o ngā peka nei. I roto i te ūpoko tuatoru, ka tirohia ētehi kōrero mō ia hapū o Ngā Ruahine. Mai i ngā kōrero mō ngā rohe whenua o ia hapū, ki ngā kōrero mō ngā marae me ngā pā tawhito o ērā hapū, ā, tae atu hoki ki ngā tūpuna rongonui me ā rātou mahi whakaari.  
Hei te mutunga, ka herea katoatia ngā kōrero o te tuhinga nei i raro i te ūpoko tuawhā, kia kite ai , he aha ngā hua e pūawai ai i tēnei tuhinga. Koinei te whakamutunga o ngā kōrero, arā ko te whakakapīnga o ngā kōrero.  
Ko tēnei tuhingaroa, he whakaemi kōrero mai i ngā kaumātua o Ngā Ruahine, ā, he mea tango mai hoki ētehi kōrero i ngā pukapuka, ko ēnei kōrero kua whakatakotoria i roto i tēnei tuhingaroa hei whakawhāriki iho ki te aroaro o te tini. Ko te tūmanako, he tuhinga rangahau tēnei hei āwhina i ngā rangatahi o Ngā Ruahine, kia rongo ai rātou i ngā kōrero kāore i te tino rangona i ēnei rā, kia mau hoki ngā kōrero a ngā kaumātua o Ngā Ruahine, kei ngaro. Erangi, he whakamōhio tēnei, he tīmatanga anahe tēnei tuhingaroa mō te kaupapa nei. Ko te tūmanako, he hua anō ka puta i tēnei rangahau, hei whāngai i ngā manu hiakai o Ngā Ruahine.

year: 1998