title: Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discrimination

authors: Smith, Kirsty Fiona

subjects: Cryptic invasion

abstract: Mitochondrial cytochrome oxidase I (COI) gene sequencing (DNA barcoding) of Ciona specimens from New Zealand (NZ) led to the first record of the solitary ascidian Ciona savignyi in the Southern Hemisphere. We sought to quantify C. savignyi COI genetic diversity around the NZ archipelago and to compare this with diversity within C. savignyi's native range in the north-west Pacific. Ciona savignyi specimens were collected from two NZ sites and from three sites around Japan. COI sequences (595 bp) were amplified and measures of genetic diversity were calculated. Based on differences between their COI sequences we developed a PCR-based assay to distinguish C. savignyi from the morphologically similar C. intestinalis. A total of 12 C. savignyi COI haplotypes were recovered from the 76 samples. Of the four haplotypes observed in NZ, two were unique. From the 10 haplotypes observed in the Japan samples, eight were unique. The C. savignyi populations in Japan were found to contain higher haplotype diversity when compared with those in NZ. The NZ samples contained only a small subset of the haplotype variation of the Japan samples, however, NZ samples did harbor two haplotypes not observed in the Japan samples. A PCR-based assay developed from the COI sequences was able to reliably discriminate the two Ciona species. The low COI genetic diversity within the two NZ C. savignyi populations sampled is consistent with a founder effect associated loss of genetic diversity. The robust PCR-based assay for distinguishing C. savignyi and C. intestinalis may find application in ecological and taxonomic studies and can be applied to both archival materials and live animals.

text: Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discrimination Mitochondrial cytochrome oxidase I (COI) gene sequencing (DNA barcoding) of Ciona specimens from New Zealand (NZ) led to the first record of the solitary ascidian Ciona savignyi in the Southern Hemisphere. We sought to quantify C. savignyi COI genetic diversity around the NZ archipelago and to compare this with diversity within C. savignyi's native range in the north-west Pacific. Ciona savignyi specimens were collected from two NZ sites and from three sites around Japan. COI sequences (595 bp) were amplified and measures of genetic diversity were calculated. Based on differences between their COI sequences we developed a PCR-based assay to distinguish C. savignyi from the morphologically similar C. intestinalis. A total of 12 C. savignyi COI haplotypes were recovered from the 76 samples. Of the four haplotypes observed in NZ, two were unique. From the 10 haplotypes observed in the Japan samples, eight were unique. The C. savignyi populations in Japan were found to contain higher haplotype diversity when compared with those in NZ. The NZ samples contained only a small subset of the haplotype variation of the Japan samples, however, NZ samples did harbor two haplotypes not observed in the Japan samples. A PCR-based assay developed from the COI sequences was able to reliably discriminate the two Ciona species. The low COI genetic diversity within the two NZ C. savignyi populations sampled is consistent with a founder effect associated loss of genetic diversity. The robust PCR-based assay for distinguishing C. savignyi and C. intestinalis may find application in ecological and taxonomic studies and can be applied to both archival materials and live animals.

year: 2012

title: Maximising potential: The psychological effects of the youth development programme Project K

authors: Furness, Kirsty

subjects: Adolescene

abstract: Project K is a positive youth development programme targeting 13-15 year old students with low self-efficacy. It involves three components: wilderness adventure, community challenge and individual mentoring. This longitudinal study aimed to investigate changes in self-efficacy, resilience, connectedness and wellbeing in students participating in Project K. Eighty students (59% male) were recruited from five secondary schools across the North Island of New Zealand for a quasi-experimental study. Participants displaying delinquent behaviour, self-harm, suicidal ideation, or an eating disorder were excluded. Over 14 months, six waves of measurement were completed by Project K participants (n = 49), while four waves of measurement were completed by a control group (n = 31). Analyses using multilevel models showed that completion of Project K had substantial positive effects on self-efficacy, resilience, and wellbeing, although the effect on connectedness was not significant. We conclude that Project K appears to be an effective positive youth development programme for adolescents with low self-efficacy.

text: Maximising potential: The psychological effects of the youth development programme Project K Project K is a positive youth development programme targeting 13-15 year old students with low self-efficacy. It involves three components: wilderness adventure, community challenge and individual mentoring. This longitudinal study aimed to investigate changes in self-efficacy, resilience, connectedness and wellbeing in students participating in Project K. Eighty students (59% male) were recruited from five secondary schools across the North Island of New Zealand for a quasi-experimental study. Participants displaying delinquent behaviour, self-harm, suicidal ideation, or an eating disorder were excluded. Over 14 months, six waves of measurement were completed by Project K participants (n = 49), while four waves of measurement were completed by a control group (n = 31). Analyses using multilevel models showed that completion of Project K had substantial positive effects on self-efficacy, resilience, and wellbeing, although the effect on connectedness was not significant. We conclude that Project K appears to be an effective positive youth development programme for adolescents with low self-efficacy.

year: 2017

title: Tū Māori Ake: Seeking which is above.

authors: Maxwell, Te Kahautu

abstract: Te Waka Āwhina 2015 Seeking that which is above Kaupapa - Philosophy o Aotearoa “Tū Māori ake” E ngā mana, e ngā reo, e ngā karangatanga, nei rā te powhiri ki a koutou katoa ‘MĀ TE MĀORI, MŌ TE MĀORI’ Te Waka Āwhina o Aotearoa National Training Hui by Māori for Māori within local government Āwhea: 2 – 4 February 2015 Ki whea: The Gallagher Academy of Performing Arts, The University of Waikato, Hamilton Hau kāinga: Te Puku o te Ika.

text: Tū Māori Ake: Seeking which is above. Te Waka Āwhina 2015 Seeking that which is above Kaupapa - Philosophy o Aotearoa “Tū Māori ake” E ngā mana, e ngā reo, e ngā karangatanga, nei rā te powhiri ki a koutou katoa ‘MĀ TE MĀORI, MŌ TE MĀORI’ Te Waka Āwhina o Aotearoa National Training Hui by Māori for Māori within local government Āwhea: 2 – 4 February 2015 Ki whea: The Gallagher Academy of Performing Arts, The University of Waikato, Hamilton Hau kāinga: Te Puku o te Ika.

year: 2015-02-03