title: Proteomes and Signalling Pathways of Antler Stem Cells

authors: Li, Chunyi

abstract: As the only known example of complete organ regeneration in mammals, deer antler in the growing or velvet phase is of major interest in developmental biology. This regeneration event initiates from self-renewing antler stem cells that exhibit pluripotency. At present, it remains unclear how the activation and quiescence of antler stem cells are regulated. Therefore, in the present study proteins that were differentially expressed between the antler stem cells and somatic cells (facial periosteum) were identified by a gel-based proteomic technique, and analysed using Ingenuity Pathway Analysis software. Several molecular pathways (PI3K/Akt, ERK/MAPK, p38 MAPK, etc.) were found to be activated during proliferation. Also expressed were the transcription factors POU5F1, SOX2, NANOG and MYC, which are key markers of embryonic stem cells. Expression of these proteins was confirmed in both cultured cells and fresh tissues by Western blot analysis. Therefore, the molecular pathways and transcription factors identified in the current study are common to embryonic and adult stem cells. However, expression of embryonic stem cell transcription factors would suggest that antler stem cells are, potentially, an intermediary stem cell type between embryonic and the more specialized tissue-specific stem cells like those residing in muscle, fat or from a hematopoietic origin. The retention of this embryonic, pluripotent lineage may be of fundamental importance for the subsequent regenerative capacity of antlers.

text: Proteomes and Signalling Pathways of Antler Stem Cells As the only known example of complete organ regeneration in mammals, deer antler in the growing or velvet phase is of major interest in developmental biology. This regeneration event initiates from self-renewing antler stem cells that exhibit pluripotency. At present, it remains unclear how the activation and quiescence of antler stem cells are regulated. Therefore, in the present study proteins that were differentially expressed between the antler stem cells and somatic cells (facial periosteum) were identified by a gel-based proteomic technique, and analysed using Ingenuity Pathway Analysis software. Several molecular pathways (PI3K/Akt, ERK/MAPK, p38 MAPK, etc.) were found to be activated during proliferation. Also expressed were the transcription factors POU5F1, SOX2, NANOG and MYC, which are key markers of embryonic stem cells. Expression of these proteins was confirmed in both cultured cells and fresh tissues by Western blot analysis. Therefore, the molecular pathways and transcription factors identified in the current study are common to embryonic and adult stem cells. However, expression of embryonic stem cell transcription factors would suggest that antler stem cells are, potentially, an intermediary stem cell type between embryonic and the more specialized tissue-specific stem cells like those residing in muscle, fat or from a hematopoietic origin. The retention of this embryonic, pluripotent lineage may be of fundamental importance for the subsequent regenerative capacity of antlers.

year: 2012

title: The acute and longer-term effects of cold water immersion in highly-trained volleyball athletes during an intense training block

authors: Tavares, Francisco

abstract: Background: The use of cold water immersion (CWI) as a recovery strategy following exercise has drawn mixed findings over the last few decades. The purpose of the current study was two-fold; (1) to determine the acute effects of CWI within the training week, and (2) to investigate the longer-term effects of CWI over a 16-day period.

Methods: In a randomized, controlled trial, 13 national-level volleyball athletes were allocated to two groups, an experimental (CWI, n = 7) and a control group (n = 6) during a 3-week national training camp. The experimental group were exposed to a CWI protocol after the last training session of each day (12 CWI sessions). Measures of lower (countermovement jump and squat jump height) and upper-body (medicine ball throw distance) power were collected pre- and post-training camp. Perceptual and neuromuscular performance measures (countermovement jump) were obtained during the training camp.

Results: No significant differences between groups were observed for any measure (p > 0.05), however, small effect sizes were observed between experimental and control groups on day two of weeks one and two. Three weeks of training resulted in a significant decrease in countermovement jump height in the control group. A moderate effect size (d = 0.65) was found for countermovement jump performance between the experimental and control groups.

Conclusion: Cold water immersion seems to provide little benefit to recovery in the acute setting (within the training week), however, chronically, there was a trend toward a benefit when implementing cold water immersion in well-trained volleyball athletes over 16 days.

text: The acute and longer-term effects of cold water immersion in highly-trained volleyball athletes during an intense training block Background: The use of cold water immersion (CWI) as a recovery strategy following exercise has drawn mixed findings over the last few decades. The purpose of the current study was two-fold; (1) to determine the acute effects of CWI within the training week, and (2) to investigate the longer-term effects of CWI over a 16-day period.

Methods: In a randomized, controlled trial, 13 national-level volleyball athletes were allocated to two groups, an experimental (CWI, n = 7) and a control group (n = 6) during a 3-week national training camp. The experimental group were exposed to a CWI protocol after the last training session of each day (12 CWI sessions). Measures of lower (countermovement jump and squat jump height) and upper-body (medicine ball throw distance) power were collected pre- and post-training camp. Perceptual and neuromuscular performance measures (countermovement jump) were obtained during the training camp.

Results: No significant differences between groups were observed for any measure (p > 0.05), however, small effect sizes were observed between experimental and control groups on day two of weeks one and two. Three weeks of training resulted in a significant decrease in countermovement jump height in the control group. A moderate effect size (d = 0.65) was found for countermovement jump performance between the experimental and control groups.

Conclusion: Cold water immersion seems to provide little benefit to recovery in the acute setting (within the training week), however, chronically, there was a trend toward a benefit when implementing cold water immersion in well-trained volleyball athletes over 16 days.

year: 2020

title: Me ko Māui: Harnessing the sun to provide better outcomes for marae and communities

authors: Greensill, Waikauri

abstract: Marae are the cultural centres of Māori communities, providing a space for important gatherings both joyful and sad. With the advent of solar energy capture technologies and rising electricity bills, many marae are becoming interested in whether a solar powered system will be beneficial for them. However, marae have unique electricity demand profiles, distinct from commonly modelled building types such as residential or commercial buildings. It is thus difficult to estimate an appropriate solar and battery system size based on annual or even monthly electricity usage data, which may be the only data readily available, requiring more in-depth analysis at a highly site-specific level to find an answer. This thesis aims to increase understanding around how solar power could benefit marae, first by investigating the characteristic electricity demand profile of marae, then by observing how proposed system configurations meet the demand. The potential community benefits arising from such a system was also investigated. Using a year’s worth of half-hourly data from three marae, five characteristic day types were found in the analysis of the demand profile, namely a “Non-Event” profile that maintained a low baseload and accounted for 70% of the days in a year, a “1-Day Event” profile that lasted only a few hours, and for events spanning multiple days a “Start”, “Middle” and “End” day profile that varied greatly in demand magnitude. The demand profiles were then used to develop an annual demand profile for an average marae that was broadly representative of a wide range of marae. Different configurations of solar and battery powered systems were then applied to the annual demand and a technoeconomic analysis performed to determine whether a specific configuration met the needs of the average marae or not, which then produced a range of acceptable system sizes based on the needs specified. Not many solar and battery configurations resulted in positive economic returns for the marae alone, but widening the scope of the analysis to the surrounding community allows other interests such as local grid resilience to be considered, some of which may justify a marae’s decision to invest in a non-economic system to gain better community benefits. While investigating the use of peer-to-peer energy trading and community energy scenarios, sharing excess energy generated by a system with the wider community was also found to improve the economic outlook of solar for marae.

text: Me ko Māui: Harnessing the sun to provide better outcomes for marae and communities Marae are the cultural centres of Māori communities, providing a space for important gatherings both joyful and sad. With the advent of solar energy capture technologies and rising electricity bills, many marae are becoming interested in whether a solar powered system will be beneficial for them. However, marae have unique electricity demand profiles, distinct from commonly modelled building types such as residential or commercial buildings. It is thus difficult to estimate an appropriate solar and battery system size based on annual or even monthly electricity usage data, which may be the only data readily available, requiring more in-depth analysis at a highly site-specific level to find an answer. This thesis aims to increase understanding around how solar power could benefit marae, first by investigating the characteristic electricity demand profile of marae, then by observing how proposed system configurations meet the demand. The potential community benefits arising from such a system was also investigated. Using a year’s worth of half-hourly data from three marae, five characteristic day types were found in the analysis of the demand profile, namely a “Non-Event” profile that maintained a low baseload and accounted for 70% of the days in a year, a “1-Day Event” profile that lasted only a few hours, and for events spanning multiple days a “Start”, “Middle” and “End” day profile that varied greatly in demand magnitude. The demand profiles were then used to develop an annual demand profile for an average marae that was broadly representative of a wide range of marae. Different configurations of solar and battery powered systems were then applied to the annual demand and a technoeconomic analysis performed to determine whether a specific configuration met the needs of the average marae or not, which then produced a range of acceptable system sizes based on the needs specified. Not many solar and battery configurations resulted in positive economic returns for the marae alone, but widening the scope of the analysis to the surrounding community allows other interests such as local grid resilience to be considered, some of which may justify a marae’s decision to invest in a non-economic system to gain better community benefits. While investigating the use of peer-to-peer energy trading and community energy scenarios, sharing excess energy generated by a system with the wider community was also found to improve the economic outlook of solar for marae.

year: 2024