title: Enzyme activity and dynamics: xylanase activity in the absence of fast anharmonic dynamics

authors: Dunn, Rachel V.

subjects: cryoenzymology

abstract: The activity and dynamics of a simple, single subunit enzyme, the xylanase from Thermotoga maritima strain Fj SS3B.1 have been measured under similar conditions, from -70 to +10 °C. The internal motions of the enzyme, as evidenced by neutron scattering, undergo a sharp transition within this temperature range; they show no evidence for picosecond-timescale anharmonic behaviour (e.g. local diffusive motions or jumps between alternative conformations) at temperatures below -50 °C, whereas these motions are strongly activated at higher temperatures. The activity follows Arrhenius behaviour over the whole of the temperature range investigated, -70 to +10 °C. The results indicate that a temperature range exists over which the enzyme rate-limiting step is independent of fast anharmonic dynamics.

text: Enzyme activity and dynamics: xylanase activity in the absence of fast anharmonic dynamics The activity and dynamics of a simple, single subunit enzyme, the xylanase from Thermotoga maritima strain Fj SS3B.1 have been measured under similar conditions, from -70 to +10 °C. The internal motions of the enzyme, as evidenced by neutron scattering, undergo a sharp transition within this temperature range; they show no evidence for picosecond-timescale anharmonic behaviour (e.g. local diffusive motions or jumps between alternative conformations) at temperatures below -50 °C, whereas these motions are strongly activated at higher temperatures. The activity follows Arrhenius behaviour over the whole of the temperature range investigated, -70 to +10 °C. The results indicate that a temperature range exists over which the enzyme rate-limiting step is independent of fast anharmonic dynamics.

year: 2000

title: Burrows and Carter Statute Law in New Zealand by Ross Carter [Book Review]

authors: Green, Cheryl

abstract: This article reviews the book “Burrows and Carter Statute Law in New Zealand”, by Ross Carter.

text: Burrows and Carter Statute Law in New Zealand by Ross Carter [Book Review] This article reviews the book “Burrows and Carter Statute Law in New Zealand”, by Ross Carter.

year: 2016

title: Te mātauranga whakatere waka

authors: Tuaupiki, Jackie Whetumarama

abstract: Hei tēnei tuhinga e whai ake nei, ka tirohia te hangarau waka a ngā tūpuna o Te Moana nui a Kiwa i whakawhiti ai rātou ki ō rātou moutere tae atu ki te mātauranga whakatere waka me te arotahi o te titiro ki te whakatinanatanga o aua mātauranga. Ka tāmutu i konā, ka pūhia te titiro i runga i ngā huarere o Poronīhia e kite ai tātou i ngā āhuatanga o Tāwhirimātea i ōna moutere me ngā wero ki te whakatere waka i ērā huarere.

text: Te mātauranga whakatere waka Hei tēnei tuhinga e whai ake nei, ka tirohia te hangarau waka a ngā tūpuna o Te Moana nui a Kiwa i whakawhiti ai rātou ki ō rātou moutere tae atu ki te mātauranga whakatere waka me te arotahi o te titiro ki te whakatinanatanga o aua mātauranga. Ka tāmutu i konā, ka pūhia te titiro i runga i ngā huarere o Poronīhia e kite ai tātou i ngā āhuatanga o Tāwhirimātea i ōna moutere me ngā wero ki te whakatere waka i ērā huarere.

year: 2017