title: Investigating the effect of a topical carnosine gel on 1,500 m rowing performance in experienced club-level rowers

authors: Rountree, James

subjects: rowing performance

abstract: It is well established that beta-alanine supplementation increases muscle carnosine levels and, in many cases, improves high-intensity exercise performance. Recent research has shown that applying a topical carnosine gel to the skin may be an effective alternative method to increase muscle carnosine and improve high-intensity exercise performance. However, there is currently no research on the effect of topical carnosine on rowing performance.  
  
This thesis is separated into three chapters. Chapter One is a review of the literature regarding the physiology of muscular fatigue in high-intensity exercise, the relationship between muscle typology and buffering capacity, supplement-based interventions to elevate buffering capacity, the ergogenic properties of carnosine (with particular relevance to rowing performance), and the novel use of topical carnosine to improve high-intensity exercise performance. The literature shows that an accumulation of hydrogen ions, a by-product of anaerobic glycolysis metabolism, negatively impacts muscle function, causes pain in the muscle, and decreases muscle pH, ultimately reducing exercise performance. Multiple studies found that raising the amount of intramuscular carnosine improved performance because carnosine is a pH buffer and may increase the calcium ion sensitivity within the muscle. Due to the limitations of direct carnosine ingestion, an increase in muscle carnosine is traditionally achieved via beta-alanine supplementation, which can cause small performance improvements in high-intensity exercise, such as rowing. Topical carnosine is a novel, more efficient alternative to elevate muscle carnosine; however, there is limited, conflicting evidence of its effect on high-intensity exercise performance, thus, more research is needed.   
 
Chapter Two investigated the effect of a topical carnosine gel on 1,500 m rowing ergometer time trial (TT) performance. Thirteen club-level rowers (11 male, 2 female) were randomly allocated into Group A or B, from which a crossover design was used to measure the effect of the intervention. In a double-blind fashion, participants applied 15 mL of a topical carnosine gel (CAR) or an ultrasound placebo gel (PLA) to their back and limb muscles 45 minutes before each TT, which were separated by one week. The paired dependent t-test and Cohen’s effect sizes found that topical carnosine had a trivial, non-significant effect on TT performance (CAR 300.5 ± 23.1 s; PLA 300.4 ± 24.2 s, p = 0.945, d = 0.004), 500 m split times 500 m (p = 0.808, d = 0.009; 1000 m p = 0.830, d = 0.011; 1500 m p = 0.849, d = 0.020), and rated perceived exertion (p = 1.000, d = 0.000). These results suggest that topical carnosine does not affect 1,500 m rowing performance. After the intervention was deemed ineffective, the reliability of the 1,500 m TT was calculated using Trial 1 vs Trial 2 (Trial 1: 301 ± 23.8 s, Trial 2: 300.0 ± 24.0 s; p =0.114; d = 0.074; trivial effect size; TE = 2.8 s; ICC = 0.985). The reliability statistics suggest that the 1,500 m TT performed on a Concept 2 rowing ergometer is a reliable measure of rowing performance.  

Chapter Three summarises the findings from Chapter Two, identifies the strengths and weaknesses of our research, and provides recommendations for future research. Overall, this thesis identified gaps in the literature regarding carnosine and high-intensity exercise, and found links between papers on muscle typology, buffer capacity, beta-alanine, and topical carnosine that can be used to develop rationales for future research. It was clear that, under the conditions tested in this experimental protocol, topical carnosine does not affect performance in the 1,500 m rowing ergometer TT in experienced club-level rowers.

text: Investigating the effect of a topical carnosine gel on 1,500 m rowing performance in experienced club-level rowers It is well established that beta-alanine supplementation increases muscle carnosine levels and, in many cases, improves high-intensity exercise performance. Recent research has shown that applying a topical carnosine gel to the skin may be an effective alternative method to increase muscle carnosine and improve high-intensity exercise performance. However, there is currently no research on the effect of topical carnosine on rowing performance.  
  
This thesis is separated into three chapters. Chapter One is a review of the literature regarding the physiology of muscular fatigue in high-intensity exercise, the relationship between muscle typology and buffering capacity, supplement-based interventions to elevate buffering capacity, the ergogenic properties of carnosine (with particular relevance to rowing performance), and the novel use of topical carnosine to improve high-intensity exercise performance. The literature shows that an accumulation of hydrogen ions, a by-product of anaerobic glycolysis metabolism, negatively impacts muscle function, causes pain in the muscle, and decreases muscle pH, ultimately reducing exercise performance. Multiple studies found that raising the amount of intramuscular carnosine improved performance because carnosine is a pH buffer and may increase the calcium ion sensitivity within the muscle. Due to the limitations of direct carnosine ingestion, an increase in muscle carnosine is traditionally achieved via beta-alanine supplementation, which can cause small performance improvements in high-intensity exercise, such as rowing. Topical carnosine is a novel, more efficient alternative to elevate muscle carnosine; however, there is limited, conflicting evidence of its effect on high-intensity exercise performance, thus, more research is needed.   
 
Chapter Two investigated the effect of a topical carnosine gel on 1,500 m rowing ergometer time trial (TT) performance. Thirteen club-level rowers (11 male, 2 female) were randomly allocated into Group A or B, from which a crossover design was used to measure the effect of the intervention. In a double-blind fashion, participants applied 15 mL of a topical carnosine gel (CAR) or an ultrasound placebo gel (PLA) to their back and limb muscles 45 minutes before each TT, which were separated by one week. The paired dependent t-test and Cohen’s effect sizes found that topical carnosine had a trivial, non-significant effect on TT performance (CAR 300.5 ± 23.1 s; PLA 300.4 ± 24.2 s, p = 0.945, d = 0.004), 500 m split times 500 m (p = 0.808, d = 0.009; 1000 m p = 0.830, d = 0.011; 1500 m p = 0.849, d = 0.020), and rated perceived exertion (p = 1.000, d = 0.000). These results suggest that topical carnosine does not affect 1,500 m rowing performance. After the intervention was deemed ineffective, the reliability of the 1,500 m TT was calculated using Trial 1 vs Trial 2 (Trial 1: 301 ± 23.8 s, Trial 2: 300.0 ± 24.0 s; p =0.114; d = 0.074; trivial effect size; TE = 2.8 s; ICC = 0.985). The reliability statistics suggest that the 1,500 m TT performed on a Concept 2 rowing ergometer is a reliable measure of rowing performance.  

Chapter Three summarises the findings from Chapter Two, identifies the strengths and weaknesses of our research, and provides recommendations for future research. Overall, this thesis identified gaps in the literature regarding carnosine and high-intensity exercise, and found links between papers on muscle typology, buffer capacity, beta-alanine, and topical carnosine that can be used to develop rationales for future research. It was clear that, under the conditions tested in this experimental protocol, topical carnosine does not affect performance in the 1,500 m rowing ergometer TT in experienced club-level rowers.

year: 2025

title: A quick, reliable solution for modelling cheese chilling process

authors: Hoang, Duy

subjects: Modelling

abstract: Optimisation of the refrigerating system for cheese processing requires an accurate prediction of chilling time, product temperature distributions and heat flow. Many existing CFD models can provide the best predictions by directly solving the three-dimensional heat transfer problem within the product and for air flow around the product; however, they are time-consuming and not suitable for routine use. A one-dimensional numerical solution proposed by Ghraizi, Chumak, Onistchenko, and Terziev (1996) has been used to provide the quick answer to food processing engineers with a good accuracy. In this method, the partial differential equation describing one-dimensional non-linear unsteady heat conduction inside the product has been solved by a finite difference technique. The method can take into account the temperature-dependence of thermal properties of foods and a general shape factor was used to reflect the product geometry. The model was applied to a single block of cheese, and agar. Predicted results are compared to experimentally-measured temperature profiles as well as to results generated by the Food Product Modeller, FPM, software.

text: A quick, reliable solution for modelling cheese chilling process Optimisation of the refrigerating system for cheese processing requires an accurate prediction of chilling time, product temperature distributions and heat flow. Many existing CFD models can provide the best predictions by directly solving the three-dimensional heat transfer problem within the product and for air flow around the product; however, they are time-consuming and not suitable for routine use. A one-dimensional numerical solution proposed by Ghraizi, Chumak, Onistchenko, and Terziev (1996) has been used to provide the quick answer to food processing engineers with a good accuracy. In this method, the partial differential equation describing one-dimensional non-linear unsteady heat conduction inside the product has been solved by a finite difference technique. The method can take into account the temperature-dependence of thermal properties of foods and a general shape factor was used to reflect the product geometry. The model was applied to a single block of cheese, and agar. Predicted results are compared to experimentally-measured temperature profiles as well as to results generated by the Food Product Modeller, FPM, software.

year: 2018

title: Lake Rotokakahi: The kakahi (Hyridella menziesi) in a general framework of lake health

authors: Butterworth, Joseph

subjects: Rotokakahi

abstract: Lake Rotokakahi is a mesotrophic lake located within the Rotorua Lakes District, North Island, New Zealand. Under the legal guardianship of the Tuhourangi and Ngati Tumatawera tribes of Te Arawa it has remained closed to the public since 1948. 

Lake Rotokakahi was last monitored regularly in 1996 under the Environment Bay of Plenty (EBOP) water quality monitoring programme with only the lake outlet (Te Wairoa Stream) being monitored since that time. Water quality data collected up to 1996 suggests that there may be degradation of water quality in the lake, as indicated by declining levels of dissolved oxygen in the bottom waters.

 Lake Rotokakahi steeped in historical significance, as well as having major cultural and recreational values was well known for its abundant resources and as the name suggests, particularly for its massive supply of the freshwater mussel or kakahi (Hyridella menziesi). Freshwater mussel species worldwide are in decline however little is known on factors controlling kakahi abundance and distribution. 

The overarching objective of this thesis is update water quality data last monitored in Lake Rotokakahi in 1996 while also identifying key environmental variables thought to influence kakahi populations.  This objective is underpinned by a number of aims that include:
 	Establishment of a 12-13 month water quality programme within Lake Rotokakahi so that data is obtained for comparisons with previous water quality data (pre-1997) to allow assessment of whether there have been water quality changes in the lake.
 	Conduct a population survey of the resident kakahi population examining possible environmental factors influencing their populations.
 	Present an oral history of Lake Rotokakahi focusing on its historical significance, water quality, and collection of kairoto (food collected form lakes).

from the 18 September 2006 to 14 September 2007 monthly water sampling was carried out at a mid lake station, the lake outflow and inflow for measures of nutrients, phytoplankton, zooplankton and chlorophyll a. Vertical profiles of temperature dissolved oxygen and chlorophyll fluorescence were also taken on various part of Lake Rotokakahi.

A lengthened period of anoxia in the bottom waters during thermal stratification was recorded with increased levels of chlorophyll a in winter and reduced secchi disk depth indicating an increase in phytoplankton biomass. Nutrient concentrations remain moderate relative to historical data. On going water quality monitoring on Lake Rotokakahi is recommended to further evaluate the extent of which water quality change is occurring. This will provide a better understanding of how Lake Rotokakahi can be best managed to further preserve the lake.

On 1 March and 20 April kakahi were sampled at five sites. Large kakahi densities were distributed predominantly in depths above the hypolimnion. Chlorophyll a fluorescence and dissolved oxygen were found to be the best correlates for kakahi density and biomass respectively. Low dissolved oxygen concentrations in the hypolimnion are thought to restrict kakahi distributions to above the thermocline in periods of hypolimnetic anoxia.

text: Lake Rotokakahi: The kakahi (Hyridella menziesi) in a general framework of lake health Lake Rotokakahi is a mesotrophic lake located within the Rotorua Lakes District, North Island, New Zealand. Under the legal guardianship of the Tuhourangi and Ngati Tumatawera tribes of Te Arawa it has remained closed to the public since 1948. 

Lake Rotokakahi was last monitored regularly in 1996 under the Environment Bay of Plenty (EBOP) water quality monitoring programme with only the lake outlet (Te Wairoa Stream) being monitored since that time. Water quality data collected up to 1996 suggests that there may be degradation of water quality in the lake, as indicated by declining levels of dissolved oxygen in the bottom waters.

 Lake Rotokakahi steeped in historical significance, as well as having major cultural and recreational values was well known for its abundant resources and as the name suggests, particularly for its massive supply of the freshwater mussel or kakahi (Hyridella menziesi). Freshwater mussel species worldwide are in decline however little is known on factors controlling kakahi abundance and distribution. 

The overarching objective of this thesis is update water quality data last monitored in Lake Rotokakahi in 1996 while also identifying key environmental variables thought to influence kakahi populations.  This objective is underpinned by a number of aims that include:
 	Establishment of a 12-13 month water quality programme within Lake Rotokakahi so that data is obtained for comparisons with previous water quality data (pre-1997) to allow assessment of whether there have been water quality changes in the lake.
 	Conduct a population survey of the resident kakahi population examining possible environmental factors influencing their populations.
 	Present an oral history of Lake Rotokakahi focusing on its historical significance, water quality, and collection of kairoto (food collected form lakes).

from the 18 September 2006 to 14 September 2007 monthly water sampling was carried out at a mid lake station, the lake outflow and inflow for measures of nutrients, phytoplankton, zooplankton and chlorophyll a. Vertical profiles of temperature dissolved oxygen and chlorophyll fluorescence were also taken on various part of Lake Rotokakahi.

A lengthened period of anoxia in the bottom waters during thermal stratification was recorded with increased levels of chlorophyll a in winter and reduced secchi disk depth indicating an increase in phytoplankton biomass. Nutrient concentrations remain moderate relative to historical data. On going water quality monitoring on Lake Rotokakahi is recommended to further evaluate the extent of which water quality change is occurring. This will provide a better understanding of how Lake Rotokakahi can be best managed to further preserve the lake.

On 1 March and 20 April kakahi were sampled at five sites. Large kakahi densities were distributed predominantly in depths above the hypolimnion. Chlorophyll a fluorescence and dissolved oxygen were found to be the best correlates for kakahi density and biomass respectively. Low dissolved oxygen concentrations in the hypolimnion are thought to restrict kakahi distributions to above the thermocline in periods of hypolimnetic anoxia.

year: 2008