title: Activating whanau (extended family) processes within a community and school literacy partnership

authors: Glynn, Ted

subjects: Maori Education

abstract: "The paper describes the positive outcomes of a home and school literacy partnership in one mainstream primary school where the principal and the large majority of students, teachers, and whānau belong to one Māori hapū (sub-tribe), Ngāti Whakaue. Participating students were identified by teachers as experiencing difficulties with reading and writing. They were assigned randomly to one of two conditions: (1) participating in their school’s regular reading programme (school group) or (2) participating also in the home tutored reading and writing programme (home and school group).

Students in the home and school group improved their reading and writing outcomes over and above the outcomes achieved by students participating only in the regular school programme. There were two key reasons for the success of this partnership. The first was parents and whānau members successful implementation of both a Māori language reading tutoring programme, Tatari, Tautoko Tauawhi, and the two components of the writing programme, Tuhi Atu Tui Mai and Whakaputa Whakaaro. The second was the professional educational and cultural expertise of the home and school liaison worker, Hiro. Because of her language and cultural expertise and her mana (acknowledged authority and standing), Hiro was able to engage Māori parents and whānau actively in the home and school project. The co-constructed narrative between Hiro and Mere (the second author) provided a salient context for understanding the power of culturally preferred pedagogy in establishing effective home and school partnerships."

text: Activating whanau (extended family) processes within a community and school literacy partnership "The paper describes the positive outcomes of a home and school literacy partnership in one mainstream primary school where the principal and the large majority of students, teachers, and whānau belong to one Māori hapū (sub-tribe), Ngāti Whakaue. Participating students were identified by teachers as experiencing difficulties with reading and writing. They were assigned randomly to one of two conditions: (1) participating in their school’s regular reading programme (school group) or (2) participating also in the home tutored reading and writing programme (home and school group).

Students in the home and school group improved their reading and writing outcomes over and above the outcomes achieved by students participating only in the regular school programme. There were two key reasons for the success of this partnership. The first was parents and whānau members successful implementation of both a Māori language reading tutoring programme, Tatari, Tautoko Tauawhi, and the two components of the writing programme, Tuhi Atu Tui Mai and Whakaputa Whakaaro. The second was the professional educational and cultural expertise of the home and school liaison worker, Hiro. Because of her language and cultural expertise and her mana (acknowledged authority and standing), Hiro was able to engage Māori parents and whānau actively in the home and school project. The co-constructed narrative between Hiro and Mere (the second author) provided a salient context for understanding the power of culturally preferred pedagogy in establishing effective home and school partnerships."

year: 2004

title: Ascending the Poutama: Culturally responsive diabetes care for Māori communities

authors: Crosswell, Rebekah Jane

subjects: culturally responsive

abstract: Introduction
Type 2 diabetes (T2D) significantly impacts Māori populations in Aotearoa New Zealand (NZ) who experience health disparities including suboptimal disease management.

Aim
To explore culturally responsive approaches to supporting Māori living with T2D from the perspective of healthcare providers.

Methods
Nine semi-structured interviews were conducted with clinicians from various disciplines (general practitioner, podiatrist, exercise physiologist, three kaiāwhina, dietitian, two pharmacists); five clinicians identify as Māori, four as non-Māori. Kaupapa Māori inductive thematic analysis was used.

Results
Four key themes emerged: empowering patient-centred communication, whānau involvement, interprofessional collaboration, and culturally appropriate analogies.

Discussion
The findings underscore the need for holistic, culturally responsive T2D care that prioritises culturally appropriate communication to improve patient engagement, health outcomes and address Māori health disparities.

text: Ascending the Poutama: Culturally responsive diabetes care for Māori communities Introduction
Type 2 diabetes (T2D) significantly impacts Māori populations in Aotearoa New Zealand (NZ) who experience health disparities including suboptimal disease management.

Aim
To explore culturally responsive approaches to supporting Māori living with T2D from the perspective of healthcare providers.

Methods
Nine semi-structured interviews were conducted with clinicians from various disciplines (general practitioner, podiatrist, exercise physiologist, three kaiāwhina, dietitian, two pharmacists); five clinicians identify as Māori, four as non-Māori. Kaupapa Māori inductive thematic analysis was used.

Results
Four key themes emerged: empowering patient-centred communication, whānau involvement, interprofessional collaboration, and culturally appropriate analogies.

Discussion
The findings underscore the need for holistic, culturally responsive T2D care that prioritises culturally appropriate communication to improve patient engagement, health outcomes and address Māori health disparities.

year: 2025

title: The effects of allelic hinge variation in IgG3 antibodies on protein dynamics and effector functions

authors: Kuplich Barcellos, Marina

abstract: Antibodies play a crucial role in eliminating infections by recognising foreign antigens with high specificity and affinity. If a target is not directly neutralised, antibodies can mediate potent effector function responses by coordinating the recruitment of immune cells through their constant regions. These powerful immune functions are being widely explored in the form of IgG-based monoclonal antibody therapies for the treatment of infections, cancer, and autoimmune diseases. However, the antibody constant region exhibits significant genetic polymorphism, and new variants are rapidly being discovered by advances in genomic sequencing techniques. The documented effects of this diversity include differences in structural dynamics, thermal stability, and Fc-mediated effector functions among a number of antibody subclasses, including IgG1, IgG2, and IgG3. Some variants are reported to be a contributing factor to variability in the outcome of disease and responsiveness to vaccines in humans. The IgG3 subclass, in particular, displays striking differences in the antibody hinge region – responsible for connecting target binding with constant-region-mediated immune function. Here, exon duplications mean the hinge region can vary by as many as 45 amino acids in length. This work seeks to better understand some of the functional consequences of this unusual IgG3 variation. 

To assess the effects of allelic hinge variation on protein dynamics and effector functions, we have expressed a panel of seven anti-CD20 antibodies with unique constant region allele sequences from subclasses IgG1, IgG2, and IgG3. Sequence verified plasmids were used to express full-length antibodies in HEK293 cells at high purity. We first used small angle X-ray scattering (SAXS) to assess the variability in size and structural flexibility between the allelic variants and compared our data with previous research on the same variant panel with antiHER2 variable domain sequences. Following this structural analysis, we next undertook ADCC (antibody-dependent cellular cytotoxicity) and ADCP (antibody dependent cellular phagocytosis) assays to determine if differences in structural dynamics between the polymorphic variants could be linked to differences in effector function. These assays specifically used genome-engineered reporter cell lines to evaluate the ability of allelic variants to engage constant region binding FcyRIIIa and FcyRIIa Fc receptors, typically found on peripheral blood mononuclear cells. Activation was measured by quantifying the levels of luciferase expressed as a reporter.  

The findings provided in this study demonstrate that allelic variants with longer hinges have larger and more flexible structures and tend to present weaker ADCC and ADCP responses. The data presented here is the basis for future work on the influence of IgG constant region diversity on antibody structural dynamics and effector functions, which could contribute to improvements in the design of therapeutic antibodies.

text: The effects of allelic hinge variation in IgG3 antibodies on protein dynamics and effector functions Antibodies play a crucial role in eliminating infections by recognising foreign antigens with high specificity and affinity. If a target is not directly neutralised, antibodies can mediate potent effector function responses by coordinating the recruitment of immune cells through their constant regions. These powerful immune functions are being widely explored in the form of IgG-based monoclonal antibody therapies for the treatment of infections, cancer, and autoimmune diseases. However, the antibody constant region exhibits significant genetic polymorphism, and new variants are rapidly being discovered by advances in genomic sequencing techniques. The documented effects of this diversity include differences in structural dynamics, thermal stability, and Fc-mediated effector functions among a number of antibody subclasses, including IgG1, IgG2, and IgG3. Some variants are reported to be a contributing factor to variability in the outcome of disease and responsiveness to vaccines in humans. The IgG3 subclass, in particular, displays striking differences in the antibody hinge region – responsible for connecting target binding with constant-region-mediated immune function. Here, exon duplications mean the hinge region can vary by as many as 45 amino acids in length. This work seeks to better understand some of the functional consequences of this unusual IgG3 variation. 

To assess the effects of allelic hinge variation on protein dynamics and effector functions, we have expressed a panel of seven anti-CD20 antibodies with unique constant region allele sequences from subclasses IgG1, IgG2, and IgG3. Sequence verified plasmids were used to express full-length antibodies in HEK293 cells at high purity. We first used small angle X-ray scattering (SAXS) to assess the variability in size and structural flexibility between the allelic variants and compared our data with previous research on the same variant panel with antiHER2 variable domain sequences. Following this structural analysis, we next undertook ADCC (antibody-dependent cellular cytotoxicity) and ADCP (antibody dependent cellular phagocytosis) assays to determine if differences in structural dynamics between the polymorphic variants could be linked to differences in effector function. These assays specifically used genome-engineered reporter cell lines to evaluate the ability of allelic variants to engage constant region binding FcyRIIIa and FcyRIIa Fc receptors, typically found on peripheral blood mononuclear cells. Activation was measured by quantifying the levels of luciferase expressed as a reporter.  

The findings provided in this study demonstrate that allelic variants with longer hinges have larger and more flexible structures and tend to present weaker ADCC and ADCP responses. The data presented here is the basis for future work on the influence of IgG constant region diversity on antibody structural dynamics and effector functions, which could contribute to improvements in the design of therapeutic antibodies.

year: 2024