title: MOA: Massive Online Analysis, a framework for stream classification and clustering.

authors: Bifet, Albert

subjects: computer science

abstract: Massive Online Analysis (MOA) is a software environment for implementing algorithms and running experiments for online learning from evolving data streams. MOA is designed to deal with the challenging problem of scaling up the implementation of state of the art algorithms to real world dataset sizes. It contains collection of offline and online for both classification and clustering as well as tools for evaluation. In particular, for classification it implements boosting, bagging, and Hoeffding Trees, all with and without Naive Bayes classifiers at the leaves. For clustering, it implements StreamKM++, CluStream, ClusTree, Den-Stream, D-Stream and CobWeb. Researchers benefit from MOA by getting insights into workings and problems of different approaches, practitioners can easily apply and compare several algorithms to real world data set and settings. MOA supports bi-directional interaction with WEKA, the Waikato Environment for Knowledge Analysis, and is released under the GNU GPL license.

text: MOA: Massive Online Analysis, a framework for stream classification and clustering. Massive Online Analysis (MOA) is a software environment for implementing algorithms and running experiments for online learning from evolving data streams. MOA is designed to deal with the challenging problem of scaling up the implementation of state of the art algorithms to real world dataset sizes. It contains collection of offline and online for both classification and clustering as well as tools for evaluation. In particular, for classification it implements boosting, bagging, and Hoeffding Trees, all with and without Naive Bayes classifiers at the leaves. For clustering, it implements StreamKM++, CluStream, ClusTree, Den-Stream, D-Stream and CobWeb. Researchers benefit from MOA by getting insights into workings and problems of different approaches, practitioners can easily apply and compare several algorithms to real world data set and settings. MOA supports bi-directional interaction with WEKA, the Waikato Environment for Knowledge Analysis, and is released under the GNU GPL license.

year: 2010

title: Calcium-mediated thermostability in the subtilisin superfamily: the crystal structure of Bacillus Ak.1 protease at 1.8 å resolution

authors: Smith, Clyde A.

subjects: calcium binding

abstract: Proteins of the subtilisin superfamily (subtilases) are widely distributed through many living species, where they perform a variety of processing functions. They are also used extensively in industry. In many of these enzymes, bound calcium ions play a key role in protecting against autolysis and thermal denaturation. We have determined the crystal structure of a highly thermostable protease from Bacillus sp. Ak.1 that is strongly stabilized by calcium. The crystal structure, determined at 1.8 Å resolution (R=0.182, Rfree=0.247), reveals the presence of four bound cations, three Ca2+ and one Na+. Two of the Ca2+ binding sites, Ca-1 and Ca-2, correspond to sites also found in thermitase and the mesophilic subtilisins. The third calcium ion, however, is at a novel site that is created by two key amino acid substitutions near Ca-1, and has not been observed in any other subtilase. This site, acting cooperatively with Ca-1, appears to give substantially enhanced thermostability, compared with thermitase. Comparisons with the mesophilic subtilisins also point to the importance of aromatic clusters, reduced hydrophobic surface and constrained N and C termini in enhancing the thermostability of thermitase and Ak.1 protease. The Ak.1 protease also contains an unusual Cys-X-Cys disulfide bridge that modifies the active site cleft geometry.

text: Calcium-mediated thermostability in the subtilisin superfamily: the crystal structure of Bacillus Ak.1 protease at 1.8 å resolution Proteins of the subtilisin superfamily (subtilases) are widely distributed through many living species, where they perform a variety of processing functions. They are also used extensively in industry. In many of these enzymes, bound calcium ions play a key role in protecting against autolysis and thermal denaturation. We have determined the crystal structure of a highly thermostable protease from Bacillus sp. Ak.1 that is strongly stabilized by calcium. The crystal structure, determined at 1.8 Å resolution (R=0.182, Rfree=0.247), reveals the presence of four bound cations, three Ca2+ and one Na+. Two of the Ca2+ binding sites, Ca-1 and Ca-2, correspond to sites also found in thermitase and the mesophilic subtilisins. The third calcium ion, however, is at a novel site that is created by two key amino acid substitutions near Ca-1, and has not been observed in any other subtilase. This site, acting cooperatively with Ca-1, appears to give substantially enhanced thermostability, compared with thermitase. Comparisons with the mesophilic subtilisins also point to the importance of aromatic clusters, reduced hydrophobic surface and constrained N and C termini in enhancing the thermostability of thermitase and Ak.1 protease. The Ak.1 protease also contains an unusual Cys-X-Cys disulfide bridge that modifies the active site cleft geometry.

year: 1999

title: Grounding the state of exception in New Zealand: A case study of racial segregation in Pukekohe, Whiteness and the Carceral State

authors: Martin, Jessica

subjects: State of exception

abstract: Indigenous scholars in settler-colonial contexts have highlighted the hyper-imprisonment of Indigenous people, locating it as an extension of the colonial project. Although research on this issue in New Zealand is emerging, there is a notable gap in understanding how the carceral state has developed within this particular context. This study aims to address the following research question: How does the historical context of racial segregation in Pukekohe contribute to our understanding of the mechanisms of the carceral state in New Zealand?
To thoroughly examine the hyper-imprisonment of Māori, it is essential to unpack the historical origins of state violence. Despite existing research establishing a link between Māori over-representation in prisons and the enduring effects of colonisation, there is a lack of studies exploring the complex interplay among the carceral state, whiteness, policy, policing, and law, which collectively shape the current landscape of incarceration. This study focuses on the racial segregation in Pukekohe between 1920 and 1960 and utilises reflexive thematic analysis within a case study framework, drawing on the insights offered by Robert Bartholomew's book, No Māori Allowed (2020). The analysis centres on the theoretical concept of the state of exception, shedding light on how power was wielded by the white racial polity. Thus, providing a unique perspective on the carceral state and state-sanctioned violence.
The findings highlight the interconnectedness of various systemic control mechanisms employed by the carceral state. Housing, policing, and whiteness emerge as crucial factors in the control and confinement of Māori in Pukekohe. By emphasising the role of whiteness in suppressing this history, this study underscores the need for critical examination and recognition of white cultural imperialism as a fundamental pillar of the carceral state. Overall, this research offers significant insights into the construction of a targeted population and the enduring consequences of state-sanctioned violence, emphasising the importance of a comprehensive examination of New Zealand's racialised history in discussions on the hyper-imprisonment of Māori.

text: Grounding the state of exception in New Zealand: A case study of racial segregation in Pukekohe, Whiteness and the Carceral State Indigenous scholars in settler-colonial contexts have highlighted the hyper-imprisonment of Indigenous people, locating it as an extension of the colonial project. Although research on this issue in New Zealand is emerging, there is a notable gap in understanding how the carceral state has developed within this particular context. This study aims to address the following research question: How does the historical context of racial segregation in Pukekohe contribute to our understanding of the mechanisms of the carceral state in New Zealand?
To thoroughly examine the hyper-imprisonment of Māori, it is essential to unpack the historical origins of state violence. Despite existing research establishing a link between Māori over-representation in prisons and the enduring effects of colonisation, there is a lack of studies exploring the complex interplay among the carceral state, whiteness, policy, policing, and law, which collectively shape the current landscape of incarceration. This study focuses on the racial segregation in Pukekohe between 1920 and 1960 and utilises reflexive thematic analysis within a case study framework, drawing on the insights offered by Robert Bartholomew's book, No Māori Allowed (2020). The analysis centres on the theoretical concept of the state of exception, shedding light on how power was wielded by the white racial polity. Thus, providing a unique perspective on the carceral state and state-sanctioned violence.
The findings highlight the interconnectedness of various systemic control mechanisms employed by the carceral state. Housing, policing, and whiteness emerge as crucial factors in the control and confinement of Māori in Pukekohe. By emphasising the role of whiteness in suppressing this history, this study underscores the need for critical examination and recognition of white cultural imperialism as a fundamental pillar of the carceral state. Overall, this research offers significant insights into the construction of a targeted population and the enduring consequences of state-sanctioned violence, emphasising the importance of a comprehensive examination of New Zealand's racialised history in discussions on the hyper-imprisonment of Māori.

year: 2024