title: The inter-valley soil comparative survey: the ecology of Dry Valley edaphic microbial communities

authors: Lee, Charles Kai-Wu

subjects: Antarctica

abstract: Recent applications of molecular genetics to edaphic microbial communities of the McMurdo Dry Valleys and elsewhere have rejected a long-held belief that Antarctic soils contain extremely limited microbial diversity. The Inter-Valley Soil Comparative Survey aims to elucidate the factors shaping these unique microbial communities and their biogeography by integrating molecular genetic approaches with biogeochemical analyses. Although the microbial communities of Dry Valley soils may be complex, there is little doubt that the ecosystem's food web is relatively simple, and evidence suggests that physicochemical conditions may have the dominant role in shaping microbial communities. To examine this hypothesis, bacterial communities from representative soil samples collected in four geographically disparate Dry Valleys were analyzed using molecular genetic tools, including pyrosequencing of 16S rRNA gene PCR amplicons. Results show that the four communities are structurally and phylogenetically distinct, and possess significantly different levels of diversity. Strikingly, only 2 of 214 phylotypes were found in all four valleys, challenging a widespread assumption that the microbiota of the Dry Valleys is composed of a few cosmopolitan species. Analysis of soil geochemical properties indicated that salt content, alongside altitude and Cu2+, was significantly correlated with differences in microbial communities. Our results indicate that the microbial ecology of Dry Valley soils is highly localized and that physicochemical factors potentially have major roles in shaping the microbiology of ice-free areas of Antarctica. These findings hint at links between Dry Valley glacial geomorphology and microbial ecology, and raise previously unrecognized issues related to environmental management of this unique ecosystem.

text: The inter-valley soil comparative survey: the ecology of Dry Valley edaphic microbial communities Recent applications of molecular genetics to edaphic microbial communities of the McMurdo Dry Valleys and elsewhere have rejected a long-held belief that Antarctic soils contain extremely limited microbial diversity. The Inter-Valley Soil Comparative Survey aims to elucidate the factors shaping these unique microbial communities and their biogeography by integrating molecular genetic approaches with biogeochemical analyses. Although the microbial communities of Dry Valley soils may be complex, there is little doubt that the ecosystem's food web is relatively simple, and evidence suggests that physicochemical conditions may have the dominant role in shaping microbial communities. To examine this hypothesis, bacterial communities from representative soil samples collected in four geographically disparate Dry Valleys were analyzed using molecular genetic tools, including pyrosequencing of 16S rRNA gene PCR amplicons. Results show that the four communities are structurally and phylogenetically distinct, and possess significantly different levels of diversity. Strikingly, only 2 of 214 phylotypes were found in all four valleys, challenging a widespread assumption that the microbiota of the Dry Valleys is composed of a few cosmopolitan species. Analysis of soil geochemical properties indicated that salt content, alongside altitude and Cu2+, was significantly correlated with differences in microbial communities. Our results indicate that the microbial ecology of Dry Valley soils is highly localized and that physicochemical factors potentially have major roles in shaping the microbiology of ice-free areas of Antarctica. These findings hint at links between Dry Valley glacial geomorphology and microbial ecology, and raise previously unrecognized issues related to environmental management of this unique ecosystem.

year: 2011

title: Exploring the reliability of isometric benchmark tests and their relationship to performance characteristics in elite track sprint cyclists

authors: Thompson, Roné

subjects: isometrics

abstract: Benchmark tests in competitive cycling identify talent, individualise training, and monitor performance. However, varying protocols often produce conflicting results, reducing comparability. Isometric tests are prevalent, but reliability and performance correlation are underexplored. Determine the test–retest reliability of benchmark test metrics in elite track sprint cyclists and their relationship to a performance outcome. Nineteen elite track sprint cyclists (12 males, 7 females) completed seven benchmark tests across two days: modified sit-and-reach; on-bike rolling seated maximum 6-s sprints; 3-s bilateral on-bike isometrics at 90° crank angle; 3-s prone bench pull isometrics; 3-s lumbar extension isometrics; 3-s seated off-bike isometrics; and modified plank endurance. For the performance outcome, a third session within 7 days assessed peak power using an inertial load cycle ergometer. All tests showed excellent measurement consistency (ICC<inf>3,1</inf> ≥ 0.92), with low systematic bias (p ≥ 0.063), though confidence interval varied due to modest sample size. High test–retest reliability was supported by low typical errors (CV 2.0–5.5%; 9.6% for endurance). Nine benchmark metrics, including bilateral isometric measures, showed moderate to excellent correlation with peak power output (r = 0.52–0.94, p ≤ 0.023); six remained statistically significant after Bonferroni correction (p ≤ 0.005). All benchmark metrics were reliable, with six strongly and statistically significantly associated with performance.

text: Exploring the reliability of isometric benchmark tests and their relationship to performance characteristics in elite track sprint cyclists Benchmark tests in competitive cycling identify talent, individualise training, and monitor performance. However, varying protocols often produce conflicting results, reducing comparability. Isometric tests are prevalent, but reliability and performance correlation are underexplored. Determine the test–retest reliability of benchmark test metrics in elite track sprint cyclists and their relationship to a performance outcome. Nineteen elite track sprint cyclists (12 males, 7 females) completed seven benchmark tests across two days: modified sit-and-reach; on-bike rolling seated maximum 6-s sprints; 3-s bilateral on-bike isometrics at 90° crank angle; 3-s prone bench pull isometrics; 3-s lumbar extension isometrics; 3-s seated off-bike isometrics; and modified plank endurance. For the performance outcome, a third session within 7 days assessed peak power using an inertial load cycle ergometer. All tests showed excellent measurement consistency (ICC<inf>3,1</inf> ≥ 0.92), with low systematic bias (p ≥ 0.063), though confidence interval varied due to modest sample size. High test–retest reliability was supported by low typical errors (CV 2.0–5.5%; 9.6% for endurance). Nine benchmark metrics, including bilateral isometric measures, showed moderate to excellent correlation with peak power output (r = 0.52–0.94, p ≤ 0.023); six remained statistically significant after Bonferroni correction (p ≤ 0.005). All benchmark metrics were reliable, with six strongly and statistically significantly associated with performance.

year: 2025

title: Te Whiuwhiu o te Hau Maori Counselling Certificate Programme: Agency placements and supervision. Summary of and evaluation

authors: Moeke-Pickering, Taima Materangatira

subjects: counselling

abstract: Three major characteristics in determining a potentially successful practicum were identified. Firstly, the importance of determining early on whether an agency can expose the student to the necessary counselling experience that enables students to meet the requirements of the course. Secondly, the importance of exposing students to information that enable them to efficiently achieve practicum placement goals. Thirdly, the need to utilise both reflective and skills based assessment to assess the development of counselling skills, as well as the completion of tasks and duties that are required of students. Further information was gleaned about the research participants' perspectives on supervision procedures, practicum contracts, benefits of having a practicum placement and suggested training areas for the proposed TWH supervision module.

text: Te Whiuwhiu o te Hau Maori Counselling Certificate Programme: Agency placements and supervision. Summary of and evaluation Three major characteristics in determining a potentially successful practicum were identified. Firstly, the importance of determining early on whether an agency can expose the student to the necessary counselling experience that enables students to meet the requirements of the course. Secondly, the importance of exposing students to information that enable them to efficiently achieve practicum placement goals. Thirdly, the need to utilise both reflective and skills based assessment to assess the development of counselling skills, as well as the completion of tasks and duties that are required of students. Further information was gleaned about the research participants' perspectives on supervision procedures, practicum contracts, benefits of having a practicum placement and suggested training areas for the proposed TWH supervision module.

year: 1995-01-01