title: Social interactions using an electronic rabbit

authors: Zaicu, Alexandru Calin

subjects: computer science

abstract: In this project we use an electronic rabbit called Karotz, created by French company Violet. The rabbits have the ability to connect autonomously to a WI-FI network. IN this project we use Karotz to record an audio log that will contain sounds of the environment. We also programmed a way for the rabbit to send audio to its other Karotz friend. We explored if Karotz can be used to help people stay in contact with each other and to feel less homesick.

text: Social interactions using an electronic rabbit In this project we use an electronic rabbit called Karotz, created by French company Violet. The rabbits have the ability to connect autonomously to a WI-FI network. IN this project we use Karotz to record an audio log that will contain sounds of the environment. We also programmed a way for the rabbit to send audio to its other Karotz friend. We explored if Karotz can be used to help people stay in contact with each other and to feel less homesick.

year: 2013-12

title: A neural and behavioural investigation of aberrant feeding in animal models of autism spectrum disorder

authors: Harvey, Savannah

abstract: Autism spectrum disorder (ASD), a  neurodevelopmental disorder  affecting approximately 1.85% of the population, is underpinned by complex interplay between genetics and environment. Thus far, ASD research has focused predominantly on abnormal social communication, anxiety, and cognitive impairments. It omits, however, dysregulated food intake, which is the symptom present in over 60% of ASD individuals. Data suggest that feeding abnormalities include heightened food selectivity, neophobia and food refusal, as well as pica (consumption of non-food items). The current knowledge of the scope and magnitude of abnormal appetite in ASD as well as neuropathology underlying this phenomenon is extremely limited. Thus, the overarching goal of this thesis was to provide systematic evidence pertaining to aberrant food intake in ASD and identify the relevant mechanisms, by utilizing two key animal models of this disorder (one in which ASD stems from a genetic mutation, and the other, in which ASD is induced pharmacologically).
In Specific Aim 1 of this thesis, I investigated the hypothesis that ASD mutant male mice overconsume palatable foods beyond the level observed in neurotypical controls. In this animal model, ASD stems from the deletion of the contactin-associated protein like 2 (CNTNAP2) gene encoding a transmembrane neuronal protein which clusters K+ channels in the nodes of Ranvier. My studies show for the first time that Cntnap2-/- males drink more of sucrose, saccharin and intralipid solutions compared to wild-type (WT) controls. A c-Fos immunoreactivity analysis revealed that at the end of a sucrose solution meal, Cntnap2-/- mice have blunted activation in key regions associated with satiety and reward. Specifically in the central amygdala (CEA), dorsomedial hypothalamus (DMH), ventromedial hypothalamus (VMH) and the nucleus accumbens (Acb). Excessive consumption palatable sucrose  sucrose was  was ameliorated  in mutants  by intraperitoneal administration of oxytocin (OT), a satiety promoting neuropeptide whose dysregulated release is also associated with the broad symptomology of ASD, from anxiety to sociality impairments. In line with the injection experiment, the double staining for c-Fos and OT showed blunted OT neuronal activation in the hypothalamus of the mutants after a meal of sucrose. Overall, the data indicate that ASD Cntnap2-/-male mice show excessive intake of palatable foods, and it is associated with atypical neural activation (including the OT system) in response to a palatable meal. 
In Specific Aim 2, I examined whether the overconsumption of sucrose observed in male Cntnap2-/- mice also occurs in females. Research on ASD animal models often focuses on males due to concerns about oestrus driven behavioural variability and the subtler presentation of ASD-related behaviours in females. However, feeding issues in ASD affect both sexes. To address this, I evaluated sucrose intake in female Cntnap2-/- mice and compared their consummatory behaviour across different stages of the oestrous cycle. Female Cntnap2-/- mice exhibited overconsumption of sucrose, which remained consistent across all oestrous phases. This study suggests that palatability-driven overconsumption of sugar occurs in female Cntnap2-/- mice and does not appear to be influenced by oestrus phase.
In Specific Aim 3, I investigated the hypothesis that Cntnap2-/- mice exhibit heightened hyponeophagia, a common feeding issue associated with ASD. I assessed hyponeophagia in Cntnap2-/- mice both in a novel environment and in their home cage. In both contexts, Cntnap2-/- mice displayed a significantly greater latency to approach novel food compared to WTs. Notably, in the home cage more instances of presentation of that food were required for the latency in Cntnap2-/- mice to decrease to match that of WTs. The analysis of c-Fos immunoreactivity revealed different densities of activation in key brain regions associated with reward, feeding, and fear processing between Cntnap2-/- and WT mice in response to novel food presentation. These regions included the ventral tegmental area (VTA), CEA, and arcuate nucleus (ARC). These findings suggest potential impairments in neural processing of food-related novelty cues, which may contribute to the exaggerated neophobic behaviours observed.
Valproate (VPA), an anti-epileptic drug and known teratogen, induces an ASD phenotype in offspring when administered during the period of neural tube closure. Specific Aim 4 tested whether rats prenatally exposed to VPA exhibit impaired acquisition of the conditioned taste aversion (CTA), a protective learning mechanism against ingestion of foods inducing nausea/malaise. Feeding issues in ASD, such as pica, may reflect disruptions in aversion-based learning. Additionally, OT has been implicated in CTA acquisition, and VPA rats exhibit OT signaling abnormalities. Using a standard CTA paradigm with lithium chloride (LiCl) as a nausea-inducing agent, I found that VPA rats failed to acquire a CTA response. Gene expression analysis via qPCR revealed altered expression of catecholamine metabolism-, reward-, aversion-, and satiety-related genes in the CEA in LiCl-treated VPA rats compared to controls. These findings suggest that prenatal VPA exposure disrupts neural mechanisms critical for aversion-based learning, with the CEA playing a key role.
In summary, this thesis provides novel insights into the neuromolecular mechanisms underlying feeding behaviours in ASD, highlighting distinct anomalies in palatable food intake, food neophobia, and aversive learning. Using Cntnap2-/- mice and VPA-exposed rats, the studies demonstrate significant overconsumption of palatable foods in both sexes, prolonged neophobic responses, and disrupted conditioned taste aversion acquisition, all underpinned by aberrant brain activation and gene expression.

text: A neural and behavioural investigation of aberrant feeding in animal models of autism spectrum disorder Autism spectrum disorder (ASD), a  neurodevelopmental disorder  affecting approximately 1.85% of the population, is underpinned by complex interplay between genetics and environment. Thus far, ASD research has focused predominantly on abnormal social communication, anxiety, and cognitive impairments. It omits, however, dysregulated food intake, which is the symptom present in over 60% of ASD individuals. Data suggest that feeding abnormalities include heightened food selectivity, neophobia and food refusal, as well as pica (consumption of non-food items). The current knowledge of the scope and magnitude of abnormal appetite in ASD as well as neuropathology underlying this phenomenon is extremely limited. Thus, the overarching goal of this thesis was to provide systematic evidence pertaining to aberrant food intake in ASD and identify the relevant mechanisms, by utilizing two key animal models of this disorder (one in which ASD stems from a genetic mutation, and the other, in which ASD is induced pharmacologically).
In Specific Aim 1 of this thesis, I investigated the hypothesis that ASD mutant male mice overconsume palatable foods beyond the level observed in neurotypical controls. In this animal model, ASD stems from the deletion of the contactin-associated protein like 2 (CNTNAP2) gene encoding a transmembrane neuronal protein which clusters K+ channels in the nodes of Ranvier. My studies show for the first time that Cntnap2-/- males drink more of sucrose, saccharin and intralipid solutions compared to wild-type (WT) controls. A c-Fos immunoreactivity analysis revealed that at the end of a sucrose solution meal, Cntnap2-/- mice have blunted activation in key regions associated with satiety and reward. Specifically in the central amygdala (CEA), dorsomedial hypothalamus (DMH), ventromedial hypothalamus (VMH) and the nucleus accumbens (Acb). Excessive consumption palatable sucrose  sucrose was  was ameliorated  in mutants  by intraperitoneal administration of oxytocin (OT), a satiety promoting neuropeptide whose dysregulated release is also associated with the broad symptomology of ASD, from anxiety to sociality impairments. In line with the injection experiment, the double staining for c-Fos and OT showed blunted OT neuronal activation in the hypothalamus of the mutants after a meal of sucrose. Overall, the data indicate that ASD Cntnap2-/-male mice show excessive intake of palatable foods, and it is associated with atypical neural activation (including the OT system) in response to a palatable meal. 
In Specific Aim 2, I examined whether the overconsumption of sucrose observed in male Cntnap2-/- mice also occurs in females. Research on ASD animal models often focuses on males due to concerns about oestrus driven behavioural variability and the subtler presentation of ASD-related behaviours in females. However, feeding issues in ASD affect both sexes. To address this, I evaluated sucrose intake in female Cntnap2-/- mice and compared their consummatory behaviour across different stages of the oestrous cycle. Female Cntnap2-/- mice exhibited overconsumption of sucrose, which remained consistent across all oestrous phases. This study suggests that palatability-driven overconsumption of sugar occurs in female Cntnap2-/- mice and does not appear to be influenced by oestrus phase.
In Specific Aim 3, I investigated the hypothesis that Cntnap2-/- mice exhibit heightened hyponeophagia, a common feeding issue associated with ASD. I assessed hyponeophagia in Cntnap2-/- mice both in a novel environment and in their home cage. In both contexts, Cntnap2-/- mice displayed a significantly greater latency to approach novel food compared to WTs. Notably, in the home cage more instances of presentation of that food were required for the latency in Cntnap2-/- mice to decrease to match that of WTs. The analysis of c-Fos immunoreactivity revealed different densities of activation in key brain regions associated with reward, feeding, and fear processing between Cntnap2-/- and WT mice in response to novel food presentation. These regions included the ventral tegmental area (VTA), CEA, and arcuate nucleus (ARC). These findings suggest potential impairments in neural processing of food-related novelty cues, which may contribute to the exaggerated neophobic behaviours observed.
Valproate (VPA), an anti-epileptic drug and known teratogen, induces an ASD phenotype in offspring when administered during the period of neural tube closure. Specific Aim 4 tested whether rats prenatally exposed to VPA exhibit impaired acquisition of the conditioned taste aversion (CTA), a protective learning mechanism against ingestion of foods inducing nausea/malaise. Feeding issues in ASD, such as pica, may reflect disruptions in aversion-based learning. Additionally, OT has been implicated in CTA acquisition, and VPA rats exhibit OT signaling abnormalities. Using a standard CTA paradigm with lithium chloride (LiCl) as a nausea-inducing agent, I found that VPA rats failed to acquire a CTA response. Gene expression analysis via qPCR revealed altered expression of catecholamine metabolism-, reward-, aversion-, and satiety-related genes in the CEA in LiCl-treated VPA rats compared to controls. These findings suggest that prenatal VPA exposure disrupts neural mechanisms critical for aversion-based learning, with the CEA playing a key role.
In summary, this thesis provides novel insights into the neuromolecular mechanisms underlying feeding behaviours in ASD, highlighting distinct anomalies in palatable food intake, food neophobia, and aversive learning. Using Cntnap2-/- mice and VPA-exposed rats, the studies demonstrate significant overconsumption of palatable foods in both sexes, prolonged neophobic responses, and disrupted conditioned taste aversion acquisition, all underpinned by aberrant brain activation and gene expression.

year: 2025

title: Spatial and temporal characteristics of the underwater soundscape around the Motiti Protection Area: Ōtaiti/Astrolabe Reef and Motiti Island

authors: Riegel, Maria Sophie

subjects: Underwater Soundscapes

abstract: Marine ecosystems around the world have been impacted by degradation and loss of biodiversity as a result of human impacts and climate change. The monitoring and management of ecologically significant areas is vital in understanding the effects of habitat degradation on marine ecosystems. Traditional monitoring methods are often expensive, time consuming, labour intensive and invasive. Soundscapes offer a unique opportunity for monitoring habitats in a way that is cost effective, minimally invasive and efficient. The aim of this study was to use passive acoustic monitoring to create a baseline data set using ambient sound data to investigate the spatial and temporal changes in ambient sound pressure levels, and to identify biotic, abiotic and anthropogenic sound sources which make up the marine soundscapes at the Motiti Island and Ōtaiti/Astrolabe Reef. Five diverse and ecologically significant sites in the Motiti Protection Area – at Ōtaiti/Astrolabe Reef and Motiti Island, in the Bay of Plenty, New Zealand were selected and monitored over the course of a year. This provided a wide variety of data to show the diel, lunar and seasonal patterns and trends in acoustic biological activity in order to represent the ecosystems accurately. The results showed that all sites showed strong diel patterns, with a sound pressure level increase of ~6-10dB during daylight hours compared to dark hours, and evident peaks during dawn and dusk hours as a result of biological choruses composed largely of sea urchins. There were also strong seasonal trends among the five sites, with Summer having the overall highest sound pressure level compared to Spring, Autumn and Winter, this also had an effect on the peaks in dusk choruses, which were also found to be highest during Summer. Lunar phases were also found to have an impact on the sound pressure levels of all five sites, with increased sound pressure levels during the new moon, compared to sound pressure levels during the full moon. This study created a baseline data set for a culturally, ecologically, and economically valuable area. Passive acoustic monitoring was found to be an incredibly useful tool in understand the ecosystems health and biological condition, and offers an alternative to traditional monitoring which is an effective, minimally invasive, and less labour intensive way of monitoring marine ecosystems.

text: Spatial and temporal characteristics of the underwater soundscape around the Motiti Protection Area: Ōtaiti/Astrolabe Reef and Motiti Island Marine ecosystems around the world have been impacted by degradation and loss of biodiversity as a result of human impacts and climate change. The monitoring and management of ecologically significant areas is vital in understanding the effects of habitat degradation on marine ecosystems. Traditional monitoring methods are often expensive, time consuming, labour intensive and invasive. Soundscapes offer a unique opportunity for monitoring habitats in a way that is cost effective, minimally invasive and efficient. The aim of this study was to use passive acoustic monitoring to create a baseline data set using ambient sound data to investigate the spatial and temporal changes in ambient sound pressure levels, and to identify biotic, abiotic and anthropogenic sound sources which make up the marine soundscapes at the Motiti Island and Ōtaiti/Astrolabe Reef. Five diverse and ecologically significant sites in the Motiti Protection Area – at Ōtaiti/Astrolabe Reef and Motiti Island, in the Bay of Plenty, New Zealand were selected and monitored over the course of a year. This provided a wide variety of data to show the diel, lunar and seasonal patterns and trends in acoustic biological activity in order to represent the ecosystems accurately. The results showed that all sites showed strong diel patterns, with a sound pressure level increase of ~6-10dB during daylight hours compared to dark hours, and evident peaks during dawn and dusk hours as a result of biological choruses composed largely of sea urchins. There were also strong seasonal trends among the five sites, with Summer having the overall highest sound pressure level compared to Spring, Autumn and Winter, this also had an effect on the peaks in dusk choruses, which were also found to be highest during Summer. Lunar phases were also found to have an impact on the sound pressure levels of all five sites, with increased sound pressure levels during the new moon, compared to sound pressure levels during the full moon. This study created a baseline data set for a culturally, ecologically, and economically valuable area. Passive acoustic monitoring was found to be an incredibly useful tool in understand the ecosystems health and biological condition, and offers an alternative to traditional monitoring which is an effective, minimally invasive, and less labour intensive way of monitoring marine ecosystems.

year: 2022