title: An Adaptive Model-based Mutation Operator for the Wind Farm Layout Optimisation Problem

authors: Mayo, Michael

subjects: Machine learning

abstract: A novel mutation operator for the wind farm layout optimisation problem is proposed and tested. When a wind farm layout is simulated, statistics such as an individual turbine’s wake free ratio can be computed. These statistics are in addition to the global measure being optimised, for example the overall cost of energy extraction of the farm. We present algorithms that first of all build a predictive model of the wake free ratio across an entire wind farm. This model is then used inside a mutation operator to perturb turbines towards positions of high predicted wake free ratio. We evaluate our approach by comparing a 1+1 Evolutionary Strategy using this new mutation operator vs. the same algorithm with a more standard random mutation operator, and show that our new operator leads to the discovery of wind farm layouts having a statistically significantly lower cost of energy extraction.

text: An Adaptive Model-based Mutation Operator for the Wind Farm Layout Optimisation Problem A novel mutation operator for the wind farm layout optimisation problem is proposed and tested. When a wind farm layout is simulated, statistics such as an individual turbine’s wake free ratio can be computed. These statistics are in addition to the global measure being optimised, for example the overall cost of energy extraction of the farm. We present algorithms that first of all build a predictive model of the wake free ratio across an entire wind farm. This model is then used inside a mutation operator to perturb turbines towards positions of high predicted wake free ratio. We evaluate our approach by comparing a 1+1 Evolutionary Strategy using this new mutation operator vs. the same algorithm with a more standard random mutation operator, and show that our new operator leads to the discovery of wind farm layouts having a statistically significantly lower cost of energy extraction.

year: 2015

title: High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis

authors: Wu, Po-Hung

subjects: Escherichia coli

abstract: A fed-batch culture strategy for the production of recombinant Escherichia coli cells anchoring surface-displayed transglucosidase for use as a whole-cell biocatalyst for α-arbutin synthesis was developed. Lactose was used as an inducer of the recombinant protein. In fed-batch cultures, dissolved oxygen was used as the feed indicator for glucose, thus accumulation of glucose and acetate that affected the cell growth and recombinant protein production was avoided. Fed-batch fermentation with lactose induction yielded a biomass of 18 g/L, and the cells possessed very high transglucosylation activity. In the synthesis of α-arbutin by hydroquinone glucosylation, the whole-cell biocatalysts showed a specific activity of 501 nkat/g cell and produced 21 g/L of arbutin, which corresponded to 76% molar conversion. A sixfold increased productivity of whole cell biocatalysts was obtained in the fed-batch culture with lactose induction, as compared to batch culture induced by IPTG.

text: High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis A fed-batch culture strategy for the production of recombinant Escherichia coli cells anchoring surface-displayed transglucosidase for use as a whole-cell biocatalyst for α-arbutin synthesis was developed. Lactose was used as an inducer of the recombinant protein. In fed-batch cultures, dissolved oxygen was used as the feed indicator for glucose, thus accumulation of glucose and acetate that affected the cell growth and recombinant protein production was avoided. Fed-batch fermentation with lactose induction yielded a biomass of 18 g/L, and the cells possessed very high transglucosylation activity. In the synthesis of α-arbutin by hydroquinone glucosylation, the whole-cell biocatalysts showed a specific activity of 501 nkat/g cell and produced 21 g/L of arbutin, which corresponded to 76% molar conversion. A sixfold increased productivity of whole cell biocatalysts was obtained in the fed-batch culture with lactose induction, as compared to batch culture induced by IPTG.

year: 2008

title: He kākano mai i Rangiātea

authors: Masters-Awatere, Bridgette

abstract: This chapter describes reflections of learning that took place during my doctoral research. It is presented in the form of a story in which my skills as an external evaluator were sought out to evaluate Manawanui, a hapū led healthy lifestyles programme. Manawanui is run by the local Hauora Iwi Social and Health Services. Previous evaluation experiences have led to a determination to place cultural values, aims and aspirations of the iwi at the centre of this new evaluation.

text: He kākano mai i Rangiātea This chapter describes reflections of learning that took place during my doctoral research. It is presented in the form of a story in which my skills as an external evaluator were sought out to evaluate Manawanui, a hapū led healthy lifestyles programme. Manawanui is run by the local Hauora Iwi Social and Health Services. Previous evaluation experiences have led to a determination to place cultural values, aims and aspirations of the iwi at the centre of this new evaluation.

year: 2016