title: Expression of Mitochondrial Stress Protein (Cpn60) in in vitro Cultured Neonatal Porcine Islet Cells

authors: Munif, Farina

subjects: Mitochondria

abstract: Xenotransplantation of neonatal porcine islets have been demonstrated to be a viable alternative to exogenous insulin therapy for diabetes mellitus. The use of liberase has gained much success in islet isolation but factors such as batch-to-batch variation and deterioration of a batch with storage time have hampered the quality and reproducibility of tissue dissociation. Islet culture aims to optimise islet survival and insulin release in response to glucose challenge. However, it is difficult to recover and preserve islets in vitro.

Mitochondria play a key role in the secretion of insulin from pancreatic   islet cells in response to glucose stimulation. Mitochondrial dysfunction results in the induction (at mRNA and protein levels) of a molecular stress protein/heat chock protein called Cpn60. Since mitochondrial impairment will have a significant effect on the ability of in vitro cultured islet cells to function properly (i.e. release insulin in response to glucose stimulation), the expression of Cpn60 was investigated as a function of exposing neonatal porcine islet cells to various growth conditions.

The best choice of media to culture neonatal porcine islet cells was found to be not heated activated serum which showed the least levels of Cpn60 expression at mRNA levels suggesting that the cells had low levels of mitochondrial stress. Neonatal porcine islet cells would be best digested in cells digested with new liberase (QC 1050) while in 2% not heat inactivated porcine serum (NPS) as this gave the lowest levels of Cpn60 expression suggesting low levels of mitochondrial stress.

Although expression of Cpn60 at mRNA levels seems to be modulated during the growth of the porcine islet cells in media supplemented with different serum, heat treatment of serum and liberase content, no firm conclusion can be made with regard to the effect of the different treatments on mitochondrial health status until the porcine Cpn60 protein can be unequivocally identified.

text: Expression of Mitochondrial Stress Protein (Cpn60) in in vitro Cultured Neonatal Porcine Islet Cells Xenotransplantation of neonatal porcine islets have been demonstrated to be a viable alternative to exogenous insulin therapy for diabetes mellitus. The use of liberase has gained much success in islet isolation but factors such as batch-to-batch variation and deterioration of a batch with storage time have hampered the quality and reproducibility of tissue dissociation. Islet culture aims to optimise islet survival and insulin release in response to glucose challenge. However, it is difficult to recover and preserve islets in vitro.

Mitochondria play a key role in the secretion of insulin from pancreatic   islet cells in response to glucose stimulation. Mitochondrial dysfunction results in the induction (at mRNA and protein levels) of a molecular stress protein/heat chock protein called Cpn60. Since mitochondrial impairment will have a significant effect on the ability of in vitro cultured islet cells to function properly (i.e. release insulin in response to glucose stimulation), the expression of Cpn60 was investigated as a function of exposing neonatal porcine islet cells to various growth conditions.

The best choice of media to culture neonatal porcine islet cells was found to be not heated activated serum which showed the least levels of Cpn60 expression at mRNA levels suggesting that the cells had low levels of mitochondrial stress. Neonatal porcine islet cells would be best digested in cells digested with new liberase (QC 1050) while in 2% not heat inactivated porcine serum (NPS) as this gave the lowest levels of Cpn60 expression suggesting low levels of mitochondrial stress.

Although expression of Cpn60 at mRNA levels seems to be modulated during the growth of the porcine islet cells in media supplemented with different serum, heat treatment of serum and liberase content, no firm conclusion can be made with regard to the effect of the different treatments on mitochondrial health status until the porcine Cpn60 protein can be unequivocally identified.

year: 2006

title: Tongan Business Obligation: Across a sea of enterprise

authors: Fili, Rosemarie Louise

subjects: Business, obligation, challenges,

abstract: Dubbed as the only remaining monarchy in the South Pacific. The Kingdom of Tonga seeks to provide a unique history, tradition and culture. Economically though, Tonga comes in as one of the ‘least developed countries’ in the world. This research seeks to provide small to medium size businesses as the solution to reforming Tonga’s economic disposition. Prior literature has identified cultural obligation as what impedes business success. However, employing the Talanoa methodological approach, within grounded theory, secondary literature and interpretive paradigm, this research disproves cultural obligation as the only cause to business failure. Tongan business is ingrained within the Tongan way (anga-fakaTonga) of being, therefore, cultural obligation is a way of life. Furthermore, other challenges such as book keeping, management, and government assistance seeks to also contribute to the failure of Tongan business; to which business owners have provided various solutions in this study. A total of thirty-eight research samples participated in updating and conceptualizing the understanding of Tongan business especially in regards to its cultural diversity. Future research into Tongan business is need especially in comparison to western businesses.

text: Tongan Business Obligation: Across a sea of enterprise Dubbed as the only remaining monarchy in the South Pacific. The Kingdom of Tonga seeks to provide a unique history, tradition and culture. Economically though, Tonga comes in as one of the ‘least developed countries’ in the world. This research seeks to provide small to medium size businesses as the solution to reforming Tonga’s economic disposition. Prior literature has identified cultural obligation as what impedes business success. However, employing the Talanoa methodological approach, within grounded theory, secondary literature and interpretive paradigm, this research disproves cultural obligation as the only cause to business failure. Tongan business is ingrained within the Tongan way (anga-fakaTonga) of being, therefore, cultural obligation is a way of life. Furthermore, other challenges such as book keeping, management, and government assistance seeks to also contribute to the failure of Tongan business; to which business owners have provided various solutions in this study. A total of thirty-eight research samples participated in updating and conceptualizing the understanding of Tongan business especially in regards to its cultural diversity. Future research into Tongan business is need especially in comparison to western businesses.

year: 2017

title: Desirable attributes for medical graduates: The health aspirations and needs of Maaori living in Te Rohe Pootae

authors: Maikuku, Victoria Louisa Wikitoria

abstract: Health disparities for Maaori, the tangata whenua of Aotearoa New Zealand, are prevalent when compared to the health of European New Zealanders, and even more so in remote and rural communities where access to health-care services is limited. Moreover, Maaori are underrepresented in the medical workforce in which there is a serious and escalating shortage of doctors and nurses.

In the context of the University of Waikato’s proposal to establish a Graduate Entry Medical School, Te Huataki Waiora School of Health has identified the need for a collaborative approach to ‘growing’ a fit-for-purpose health workforce with the skills and commitment to care for and work with Maaori (and Pacific peoples) living in remote and rural communities. This study thus explores the health perspectives, needs, and aspirations of Maaori living in and around the King Country rural service towns of Te Kuiti and OOtorohanga. Conducted by a Ngaati Maniapoto tribal member, the location for the study is known by Maaori as Te Rohe Pootae - the lands of Rereahu/Maniapoto, to which the Waikato people sort refuge after the invasion and confiscation of their lands in the 1860s. The perspectives, needs, and aspirations of Pacific peoples living in these communities have been explored in a ‘sister’ Masters study conducted by Janina Galewski.

The present exploratory study incorporates a co-design research process underpinned by an Indigenous health framework with Kaupapa Maaori values. Kanohi ki te kanohi (face to face) semi-structured interviews were conducted with 30 community members, 11 males and 19 females aged 18 years and over. The data analysis included a combination of values, verses and holistic, and provisional coding methods (focused, thematic, descriptive) (Saldana, 2021).

The findings reveal that these community members view themselves as either rural or remote based on distance, access to services, and geographic isolation, but not population numbers. In terms of services, they want a mobile health workforce including clinics, carers, doctors, nurses; mental health workers who are equipped with the resources and time needed to reach and serve their patients; and better access to dialysis and related services. The research also identifies three healthcare strategies. When ill or injured, one-third of the group go directly to their GP; one-third try other things before seeking medical assistance, including going home to their parents and/or to their marae for comfort and advice, ‘going bush’ to (re)connect with nature, and forms of pure (cleansing rituals); and although the remaining third are registered with medical centres they do everything they can to avoid seeing a doctor at all. Instead, they rely on rongoaa and other ‘complementary and alternative’ treatments such as physiotherapists, chiropractors, osteopaths, and other healers. 

Adopting and adapting a table created by Woolley et al. (2013), the findings also revealed three sets of desirable attributes for medical graduates (and other healthcare professionals): Skill-based attributes include quality care and communication skills, knowledge-based attributes include medical knowledge and culturally appropriate knowledge, while attitude-based attributes include personality and community engagement. This framework can be used to inform a medical education curriculum, with a specific emphasis on the health needs and aspirations of Maaori living in rural and remote communities.

text: Desirable attributes for medical graduates: The health aspirations and needs of Maaori living in Te Rohe Pootae Health disparities for Maaori, the tangata whenua of Aotearoa New Zealand, are prevalent when compared to the health of European New Zealanders, and even more so in remote and rural communities where access to health-care services is limited. Moreover, Maaori are underrepresented in the medical workforce in which there is a serious and escalating shortage of doctors and nurses.

In the context of the University of Waikato’s proposal to establish a Graduate Entry Medical School, Te Huataki Waiora School of Health has identified the need for a collaborative approach to ‘growing’ a fit-for-purpose health workforce with the skills and commitment to care for and work with Maaori (and Pacific peoples) living in remote and rural communities. This study thus explores the health perspectives, needs, and aspirations of Maaori living in and around the King Country rural service towns of Te Kuiti and OOtorohanga. Conducted by a Ngaati Maniapoto tribal member, the location for the study is known by Maaori as Te Rohe Pootae - the lands of Rereahu/Maniapoto, to which the Waikato people sort refuge after the invasion and confiscation of their lands in the 1860s. The perspectives, needs, and aspirations of Pacific peoples living in these communities have been explored in a ‘sister’ Masters study conducted by Janina Galewski.

The present exploratory study incorporates a co-design research process underpinned by an Indigenous health framework with Kaupapa Maaori values. Kanohi ki te kanohi (face to face) semi-structured interviews were conducted with 30 community members, 11 males and 19 females aged 18 years and over. The data analysis included a combination of values, verses and holistic, and provisional coding methods (focused, thematic, descriptive) (Saldana, 2021).

The findings reveal that these community members view themselves as either rural or remote based on distance, access to services, and geographic isolation, but not population numbers. In terms of services, they want a mobile health workforce including clinics, carers, doctors, nurses; mental health workers who are equipped with the resources and time needed to reach and serve their patients; and better access to dialysis and related services. The research also identifies three healthcare strategies. When ill or injured, one-third of the group go directly to their GP; one-third try other things before seeking medical assistance, including going home to their parents and/or to their marae for comfort and advice, ‘going bush’ to (re)connect with nature, and forms of pure (cleansing rituals); and although the remaining third are registered with medical centres they do everything they can to avoid seeing a doctor at all. Instead, they rely on rongoaa and other ‘complementary and alternative’ treatments such as physiotherapists, chiropractors, osteopaths, and other healers. 

Adopting and adapting a table created by Woolley et al. (2013), the findings also revealed three sets of desirable attributes for medical graduates (and other healthcare professionals): Skill-based attributes include quality care and communication skills, knowledge-based attributes include medical knowledge and culturally appropriate knowledge, while attitude-based attributes include personality and community engagement. This framework can be used to inform a medical education curriculum, with a specific emphasis on the health needs and aspirations of Maaori living in rural and remote communities.

year: 2022