title: Deactivation of TEM-1 beta-Lactamase investigated by isothermal batch and non-isothermal continuous enzyme membrane reactor methods

authors: Rogers, Thomas A.

subjects: biocatalysis

abstract: The thermal deactivation of TEM-1 β-lactamase was examined using two experimental techniques: a series of isothermal batch assays and a single, continuous, non-isothermal assay in an enzyme membrane reactor (EMR). The isothermal batch-mode technique was coupled with the three-state  Equilibrium Model  of enzyme deactivation, while the results of the EMR experiment were fitted to a four-state  molten globule model . The two methods both led to the conclusions that the thermal deactivation of TEM-1 β -lactamase does not follow the Lumry-Eyring model and that the Teq of the enzyme (the point at which active and inactive states are present in equal amounts due to thermodynamic equilibrium) is at least 10 °C from the Tm (melting temperature), contrary to the idea that the true temperature optimum of a biocatalyst is necessarily close to the melting temperature.

text: Deactivation of TEM-1 beta-Lactamase investigated by isothermal batch and non-isothermal continuous enzyme membrane reactor methods The thermal deactivation of TEM-1 β-lactamase was examined using two experimental techniques: a series of isothermal batch assays and a single, continuous, non-isothermal assay in an enzyme membrane reactor (EMR). The isothermal batch-mode technique was coupled with the three-state  Equilibrium Model  of enzyme deactivation, while the results of the EMR experiment were fitted to a four-state  molten globule model . The two methods both led to the conclusions that the thermal deactivation of TEM-1 β -lactamase does not follow the Lumry-Eyring model and that the Teq of the enzyme (the point at which active and inactive states are present in equal amounts due to thermodynamic equilibrium) is at least 10 °C from the Tm (melting temperature), contrary to the idea that the true temperature optimum of a biocatalyst is necessarily close to the melting temperature.

year: 2009

title: The Behavioural Dyscontrol Scale-Validation of a computerised version in a non-clinical New Zealand population

authors: Kendall, Virginia Ruth

subjects: computerised neuropsychological testing

abstract: The objective of this study was to evaluate the validity of a new computerised version of the Behavioural Dyscontrol Scale (BDS) in comparison to the original manual version which research has shown to be a sensitive, reliable and valid measure of executive function (EF), and in particular of control over voluntary behaviour. A.J Luria deconstructed the complex construct of EF into Three Functional Units of working memory (Fluid Intelligence Factor), motor programming (Motor Programming Factor), and inappropriate response inhibition (Environmental Independence Factor) which he regarded to be predictive of a person’s capacity to function independently and autonomously in their environment. This theoretical framework and demonstrated ecological utility is what differentiates the BDS from other traditional clinical measures of EF. The subjective scoring system has restricted the use of the BDS; the development of a valid and reliable computerised version would address this limitation generating a much greater depth and range of finite objective data. Participants were 38 tertiary students who completed a demographic questionnaire, the Hamilton Anxiety and Depression self-report Scale (HADS), the Integrated Visual and Auditory Continuous Performance Test (IVACPT), Trail Making Test A and B, the manual and computerised versions of the Behavioural Dyscontrol Scale. Findings showed good levels of internal reliability and construct validity for the CBDS which yielded high sensitivity and specificity across all Three Functional Units, together with a high level of correspondence to scores generated by the manual version and by the Trails and IVACPT measures. Potential clinical applications, limitations and future directions are discussed.

text: The Behavioural Dyscontrol Scale-Validation of a computerised version in a non-clinical New Zealand population The objective of this study was to evaluate the validity of a new computerised version of the Behavioural Dyscontrol Scale (BDS) in comparison to the original manual version which research has shown to be a sensitive, reliable and valid measure of executive function (EF), and in particular of control over voluntary behaviour. A.J Luria deconstructed the complex construct of EF into Three Functional Units of working memory (Fluid Intelligence Factor), motor programming (Motor Programming Factor), and inappropriate response inhibition (Environmental Independence Factor) which he regarded to be predictive of a person’s capacity to function independently and autonomously in their environment. This theoretical framework and demonstrated ecological utility is what differentiates the BDS from other traditional clinical measures of EF. The subjective scoring system has restricted the use of the BDS; the development of a valid and reliable computerised version would address this limitation generating a much greater depth and range of finite objective data. Participants were 38 tertiary students who completed a demographic questionnaire, the Hamilton Anxiety and Depression self-report Scale (HADS), the Integrated Visual and Auditory Continuous Performance Test (IVACPT), Trail Making Test A and B, the manual and computerised versions of the Behavioural Dyscontrol Scale. Findings showed good levels of internal reliability and construct validity for the CBDS which yielded high sensitivity and specificity across all Three Functional Units, together with a high level of correspondence to scores generated by the manual version and by the Trails and IVACPT measures. Potential clinical applications, limitations and future directions are discussed.

year: 2013

title: Hāwatewate

authors: Maxwell, Te Kahautu

abstract: Hāwatewate; this composition is in remembrance of the hanging of the Ōpōtiki missionary Rev Carl Sylvius Volkner by the Hauhau, Kereopa Te Rau of Ngāti Rangiwewehi of Te Arawa. As a consequence the Te Whakatōhea chief Mokomoko was wrongly convicted and executed by hanging in Mount Eden Prison 17 May 1866. 143870 acres of Te Whakatōhea land was confiscated under the New Zealand Settlements Act 1863. This item placed third in the Whakaeke discipline and was performed by Ōpōtiki Mai Tawhiti at the Te Matatini World Championship Kapa Haka Competition 2015.

text: Hāwatewate Hāwatewate; this composition is in remembrance of the hanging of the Ōpōtiki missionary Rev Carl Sylvius Volkner by the Hauhau, Kereopa Te Rau of Ngāti Rangiwewehi of Te Arawa. As a consequence the Te Whakatōhea chief Mokomoko was wrongly convicted and executed by hanging in Mount Eden Prison 17 May 1866. 143870 acres of Te Whakatōhea land was confiscated under the New Zealand Settlements Act 1863. This item placed third in the Whakaeke discipline and was performed by Ōpōtiki Mai Tawhiti at the Te Matatini World Championship Kapa Haka Competition 2015.

year: 2015-01-08