title: Pituitary mRNA expression of FSH and LH in yellowbelly flounder (Rhombosolea leporina)

authors: Curtis, Robert

subjects: yellowbelly flounder

abstract: Recent interest has been expressed in cultivating the native, yellowbelly flounder (Rhombosolea leporina). This is largely due to its high market and cultural value. Currently, there is little research on this species, both in an aquaculture setting and from an ecological perspective. This study focused on assessing pituitary mRNA expression of two gonadotropin-beta subunit genes, follicle-stimulating hormone (fshβ) and luteinising hormone (lhβ), in wild-caught yellowbelly flounder, to better understand captive reproduction for aquaculture.

The first study characterised the expression of both pituitary fshβ and lhβ in wild-caught yellowbelly flounder in different stages of ovarian development: 1) previtellogenic (PVO), 2) early vitellogenic (EVit), 3) vitellogenic (VIT), and 4) final oocyte maturation (FOM). Both genes showed significant increases in expression between stages 1 and 4. The expression patterns of both pituitary fshβ and lhβ were similar, with a trend of increasing levels across all ovarian stages to peak at stage 4. However, peak expression levels were much greater in fshβ. This is unexpected compared to typical fish models, where pituitary lhβ expression is often many-fold greater than fshβ during oocyte maturation. The reason for this apparent anomaly in peak expression levels is unknown. However, the general profile of increasing levels of co-expressed pituitary fshβ and lhβ throughout oogenesis likely reflects the multiple-batch group synchronous ovarian development found in yellowbelly flounder.

The second study focused on quantifying fshβ and lhβ expression over a five-day period following the injection of gonadotropin-releasing hormone analogue (GnRHa). Each female yellowbelly flounder was assigned to one of four treatment groups: 25 μg/kg, 50 μg/kg, 100 μg/kg, or a control group (0 μg/kg). Although there were no statistically significant increases in fshβ or lhβ expression from day 1 to day 5, an upward trend in both lhβ and fshβ expression was observed in fish treated with 25 μg/kg of GnRHa. Similarly, an increasing trend in lhβ expression was observed in fish treated with 50 μg/kg of GnRHa. GnRHa-treated fish accounted for the highest number of individuals entering FOM, comprising 87% of all fish that reached this stage. Additionally, 85% of all fish treated with GnRHa progressed to early maturation (EM) or further. Interestingly, the 100 μg/kg GnRHa treatment resulted in a downward trend of both fshβ and lhβ expression on both days 1 and 5 post-treatment. Despite this, the 100 μg/kg GnRHa treatment exhibited the joint-highest proportion of oocytes reaching maturity (33.3%) and had 90% of individuals reaching the early migratory (EM) stage or beyond. However, none of the fish were observed to ovulate over the 5-day experimental period.

text: Pituitary mRNA expression of FSH and LH in yellowbelly flounder (Rhombosolea leporina) Recent interest has been expressed in cultivating the native, yellowbelly flounder (Rhombosolea leporina). This is largely due to its high market and cultural value. Currently, there is little research on this species, both in an aquaculture setting and from an ecological perspective. This study focused on assessing pituitary mRNA expression of two gonadotropin-beta subunit genes, follicle-stimulating hormone (fshβ) and luteinising hormone (lhβ), in wild-caught yellowbelly flounder, to better understand captive reproduction for aquaculture.

The first study characterised the expression of both pituitary fshβ and lhβ in wild-caught yellowbelly flounder in different stages of ovarian development: 1) previtellogenic (PVO), 2) early vitellogenic (EVit), 3) vitellogenic (VIT), and 4) final oocyte maturation (FOM). Both genes showed significant increases in expression between stages 1 and 4. The expression patterns of both pituitary fshβ and lhβ were similar, with a trend of increasing levels across all ovarian stages to peak at stage 4. However, peak expression levels were much greater in fshβ. This is unexpected compared to typical fish models, where pituitary lhβ expression is often many-fold greater than fshβ during oocyte maturation. The reason for this apparent anomaly in peak expression levels is unknown. However, the general profile of increasing levels of co-expressed pituitary fshβ and lhβ throughout oogenesis likely reflects the multiple-batch group synchronous ovarian development found in yellowbelly flounder.

The second study focused on quantifying fshβ and lhβ expression over a five-day period following the injection of gonadotropin-releasing hormone analogue (GnRHa). Each female yellowbelly flounder was assigned to one of four treatment groups: 25 μg/kg, 50 μg/kg, 100 μg/kg, or a control group (0 μg/kg). Although there were no statistically significant increases in fshβ or lhβ expression from day 1 to day 5, an upward trend in both lhβ and fshβ expression was observed in fish treated with 25 μg/kg of GnRHa. Similarly, an increasing trend in lhβ expression was observed in fish treated with 50 μg/kg of GnRHa. GnRHa-treated fish accounted for the highest number of individuals entering FOM, comprising 87% of all fish that reached this stage. Additionally, 85% of all fish treated with GnRHa progressed to early maturation (EM) or further. Interestingly, the 100 μg/kg GnRHa treatment resulted in a downward trend of both fshβ and lhβ expression on both days 1 and 5 post-treatment. Despite this, the 100 μg/kg GnRHa treatment exhibited the joint-highest proportion of oocytes reaching maturity (33.3%) and had 90% of individuals reaching the early migratory (EM) stage or beyond. However, none of the fish were observed to ovulate over the 5-day experimental period.

year: 2025

title: The art of mentoring: teaching that engages the soul.

authors: Gauthern, Dean

subjects: mentoring in education

abstract: This paper explores the important educational issue of the art of mentoring in education, particularly regarding teachers. Using a biographical example to illustrate, I focus upon nurturing and the soul, emotional and pedagogical qualities in mentoring, and draw attention to paradoxes and issues that make mentoring an endangered activity.

text: The art of mentoring: teaching that engages the soul. This paper explores the important educational issue of the art of mentoring in education, particularly regarding teachers. Using a biographical example to illustrate, I focus upon nurturing and the soul, emotional and pedagogical qualities in mentoring, and draw attention to paradoxes and issues that make mentoring an endangered activity.

year: 2001

title: Mana Motuhake Ringa: The non-invasive neural interface based artificial hand

authors: Owen, Mahonri W.

subjects: Prosthetics

abstract: Ten million people on the earth at any given time suffer from the loss or lack of a limb. Three million of these are upper extremity amputees. No matter the cause or reason for amputation the amputees’ life is never the same again. Independence can be lost and never regained. The loss of a limb can be an overpowering event that effects the mental, social, and physical well-being of a person. Although there has been many attempts to restore hand function through neural prosthetics there is little to no credible solutions to regain it. This thesis presents methods to restore hand function based on the human grasping system and addresses key challenges in the practical application of those methods. The contribution of this work lies in improving artificial hand function by mimicking the human grasping system. The human grasping system consists of the brain, the nervous system and the hand. The methods used investigate: biomechanical design principles for artificial hands, the limitations of machine learning in non-invasive neural interfaces (NI’s) and the development of an autonomous hand control framework for artificial hand control. 

The application of the Support Vector Machine (SVM) to a non-invasive neural interface is principally limited to binary applications in the real world. In order to overcome this limitation an autonomous approach is developed to achieve better response and accuracy from NI control frameworks. In binary applications the SVM performed well with a classification accuracy of around 80% accompanied by a response time of just under 5 seconds. When applied to multiple classifications the SVM decreased in accuracy and response dramatically. The accuracy of the autonomous hand control framework had a classification accuracy of 95% with a response time comparable to that of the SVM. This outcome has the potential to move non-invasive NI research into the real world and to restore hand function in an intuitive and meaningful way. A key learning of this research is that machine learning methods for non-invasive neural interfaces are not the best way to restore hand function for amputees. Hand function is best restored through the combining of machine learning and machine vision into a single NI based control framework. Additionally, this work is based in Mātauranga Māori with a western science approach. By so doing, amputees will be empowered, have increased independence and enjoy a better quality of life.

The scope of this work is limited to the consideration of hand function restoration for upper extremity amputees. In particular trans-radial amputees. Focus is given to non-invasive methods of control, therefore, investigations are limited to the electroencephalography (EEG) signal. Mimicking the human grasping system is a complex and difficult process, therefore, the attempts in this work to restore hand function only consider mechanisms and processes related directly to the motor control of the human hand.

text: Mana Motuhake Ringa: The non-invasive neural interface based artificial hand Ten million people on the earth at any given time suffer from the loss or lack of a limb. Three million of these are upper extremity amputees. No matter the cause or reason for amputation the amputees’ life is never the same again. Independence can be lost and never regained. The loss of a limb can be an overpowering event that effects the mental, social, and physical well-being of a person. Although there has been many attempts to restore hand function through neural prosthetics there is little to no credible solutions to regain it. This thesis presents methods to restore hand function based on the human grasping system and addresses key challenges in the practical application of those methods. The contribution of this work lies in improving artificial hand function by mimicking the human grasping system. The human grasping system consists of the brain, the nervous system and the hand. The methods used investigate: biomechanical design principles for artificial hands, the limitations of machine learning in non-invasive neural interfaces (NI’s) and the development of an autonomous hand control framework for artificial hand control. 

The application of the Support Vector Machine (SVM) to a non-invasive neural interface is principally limited to binary applications in the real world. In order to overcome this limitation an autonomous approach is developed to achieve better response and accuracy from NI control frameworks. In binary applications the SVM performed well with a classification accuracy of around 80% accompanied by a response time of just under 5 seconds. When applied to multiple classifications the SVM decreased in accuracy and response dramatically. The accuracy of the autonomous hand control framework had a classification accuracy of 95% with a response time comparable to that of the SVM. This outcome has the potential to move non-invasive NI research into the real world and to restore hand function in an intuitive and meaningful way. A key learning of this research is that machine learning methods for non-invasive neural interfaces are not the best way to restore hand function for amputees. Hand function is best restored through the combining of machine learning and machine vision into a single NI based control framework. Additionally, this work is based in Mātauranga Māori with a western science approach. By so doing, amputees will be empowered, have increased independence and enjoy a better quality of life.

The scope of this work is limited to the consideration of hand function restoration for upper extremity amputees. In particular trans-radial amputees. Focus is given to non-invasive methods of control, therefore, investigations are limited to the electroencephalography (EEG) signal. Mimicking the human grasping system is a complex and difficult process, therefore, the attempts in this work to restore hand function only consider mechanisms and processes related directly to the motor control of the human hand.

year: 2019