title: Obituary – Allan Edward Hewitt. In memory of Dr Allan Edward Hewitt - a multi-talented soil scientist

authors: Aislabie, J

subjects: obituary

abstract: Allan is remembered fondly by his colleagues in soil science throughout New Zealand and his Manaaki Whenua co-workers especially those in our Dunedin and Lincoln offices.

text: Obituary – Allan Edward Hewitt. In memory of Dr Allan Edward Hewitt - a multi-talented soil scientist Allan is remembered fondly by his colleagues in soil science throughout New Zealand and his Manaaki Whenua co-workers especially those in our Dunedin and Lincoln offices.

year: 2022-05-18

title: Comparison of cellulolytic activities in Clostridium thermocellum and Three thermophilic, cellulolytic anaerobes

authors: Reynolds, Paul H.S.

subjects: carboxymethyl cellulose

abstract: Avicelase, carboxymethyl cellulase (CMCase), and β-glucosidase activities have been compared between Clostridium thermocellum and three extremely thermophilic, cellulolytic anaerobes, isolates TP8, TP11, and KT8. The three isolates were all small, gram-negative staining, oval-ended rods which occurred singly and, at exponential phase, in long chains. They were nonflagellated and no spores were visible. The KT8 and TP11 isolates caused clumping of the cellulose during growth. In all four organisms the CMCase activity paralleled cell growth; however, in C. thermocellum and TP8 the avicelase activity did not increase until early stationary phase. Total CMCase activity in C. thermocellum was significantly higher than in the three isolates; however, avicelase activities were much more comparable among the four organisms. C. thermocellum produced higher levels of ethanol, and all four organisms produced similar concentrations of acetate. The amounts of free and bound CMCase and avicelase activities were investigated. In C. thermocellum and TP8 most of the CMCase and avicelase activities were bound to the cellulose in the medium. In contrast, most of the CMCase activity in TP1l and KT8 was free in the culture supernatant; a significant percentage of avicelase activity was also free. The TP8 isolate was also grown on a defined medium with urea as sole nitrogen source and cellulose serving as the carbon source. Under these conditions the pattern of enzyme production was the same as that in the enriched medium, although the level of that production was considerably reduced.

text: Comparison of cellulolytic activities in Clostridium thermocellum and Three thermophilic, cellulolytic anaerobes Avicelase, carboxymethyl cellulase (CMCase), and β-glucosidase activities have been compared between Clostridium thermocellum and three extremely thermophilic, cellulolytic anaerobes, isolates TP8, TP11, and KT8. The three isolates were all small, gram-negative staining, oval-ended rods which occurred singly and, at exponential phase, in long chains. They were nonflagellated and no spores were visible. The KT8 and TP11 isolates caused clumping of the cellulose during growth. In all four organisms the CMCase activity paralleled cell growth; however, in C. thermocellum and TP8 the avicelase activity did not increase until early stationary phase. Total CMCase activity in C. thermocellum was significantly higher than in the three isolates; however, avicelase activities were much more comparable among the four organisms. C. thermocellum produced higher levels of ethanol, and all four organisms produced similar concentrations of acetate. The amounts of free and bound CMCase and avicelase activities were investigated. In C. thermocellum and TP8 most of the CMCase and avicelase activities were bound to the cellulose in the medium. In contrast, most of the CMCase activity in TP1l and KT8 was free in the culture supernatant; a significant percentage of avicelase activity was also free. The TP8 isolate was also grown on a defined medium with urea as sole nitrogen source and cellulose serving as the carbon source. Under these conditions the pattern of enzyme production was the same as that in the enriched medium, although the level of that production was considerably reduced.

year: 1986

title: The importance of whakapapa for understanding fertility

authors: Rarere, Moana

abstract: The Māori fertility transition—which saw a shift from high to low birth rates between 1966 to 1976—was one of the most rapid fertility declines observed anywhere in the world. Since then, Māori fertility has hovered around replacement level (2.1 births per woman), somewhat above that of Pākehā (European) New Zealanders. More striking are differences in timing with Māori women bearing their children younger and over a longer duration. This paper sits within a broader research project that asks: What are the important influences that have sustained contemporary Māori fertility patterns? Drawing on Mana Wahine (Māori women’s discourses) and whakawhiti kōrero (interviews) with wāhine Māori (Māori women) this paper highlights whakapapa (genealogy) as an important concept in broadening and deepening our understandings of fertility, and situating individual fertility and reproduction within a broader set of relations.

text: The importance of whakapapa for understanding fertility The Māori fertility transition—which saw a shift from high to low birth rates between 1966 to 1976—was one of the most rapid fertility declines observed anywhere in the world. Since then, Māori fertility has hovered around replacement level (2.1 births per woman), somewhat above that of Pākehā (European) New Zealanders. More striking are differences in timing with Māori women bearing their children younger and over a longer duration. This paper sits within a broader research project that asks: What are the important influences that have sustained contemporary Māori fertility patterns? Drawing on Mana Wahine (Māori women’s discourses) and whakawhiti kōrero (interviews) with wāhine Māori (Māori women) this paper highlights whakapapa (genealogy) as an important concept in broadening and deepening our understandings of fertility, and situating individual fertility and reproduction within a broader set of relations.

year: 2022