title: The use of phenyl-Sepharose for the affinity purification of proteinases

authors: Prescott, Mark

subjects: proteinase

abstract: Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources. Proteinases belonging to the serine, aspartate and metallo mechanistic classes were effective retained by the media. Purification factors in the range of 2.9–60 and enzyme activity yields in excess of 88% were obtained. In some cases homogeneous enzyme was obtained from culture supernatants in a single step. A number of other proteinases from mammalian sources were also retained. The specificity of the enzyme/support interaction was studied. Proteinases complexed with peptide inhibitors (pepstatin and chymostatin) showed reduced binding to phenyl Sepharose indicating with the active site cleft whereas modification with low molecular weight active site directed inactivators such as PMSF and DAN did not, indicating that binding may not be dependent on the catalytic site. Pepsinogen and the pro-enzyme form of the serine proteinase from the thermophilic Bacillus sp. strain Ak.1 were not retained by the media and could be resolved in an efficient manner from their active counterparts.

text: The use of phenyl-Sepharose for the affinity purification of proteinases Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources. Proteinases belonging to the serine, aspartate and metallo mechanistic classes were effective retained by the media. Purification factors in the range of 2.9–60 and enzyme activity yields in excess of 88% were obtained. In some cases homogeneous enzyme was obtained from culture supernatants in a single step. A number of other proteinases from mammalian sources were also retained. The specificity of the enzyme/support interaction was studied. Proteinases complexed with peptide inhibitors (pepstatin and chymostatin) showed reduced binding to phenyl Sepharose indicating with the active site cleft whereas modification with low molecular weight active site directed inactivators such as PMSF and DAN did not, indicating that binding may not be dependent on the catalytic site. Pepsinogen and the pro-enzyme form of the serine proteinase from the thermophilic Bacillus sp. strain Ak.1 were not retained by the media and could be resolved in an efficient manner from their active counterparts.

year: 1993

title: Simultaneous phase and frequency stepping in Time-of-flight range imaging

authors: Lickfold, Carl A.

subjects: Time-of-flight

abstract: Time-of-flight (ToF) range imaging is an established full-field depth acquisition technology. ToF is prone to several forms of error, notably multi-path interference (MPI). Frequency stepping ToF mitigates these errors, but efficient computational methods suffer from bandwidth limitations. We introduce a new acquisition method based on simultaneous phase and frequency stepping, which is capable of running in real time and reduces error compared to phase stepping and frequency stepping alone.

text: Simultaneous phase and frequency stepping in Time-of-flight range imaging Time-of-flight (ToF) range imaging is an established full-field depth acquisition technology. ToF is prone to several forms of error, notably multi-path interference (MPI). Frequency stepping ToF mitigates these errors, but efficient computational methods suffer from bandwidth limitations. We introduce a new acquisition method based on simultaneous phase and frequency stepping, which is capable of running in real time and reduces error compared to phase stepping and frequency stepping alone.

year: 2019

title: Child poverty and mental health: A literature review (Commissioned for New Zealand Psychological Society and Child Poverty Action Group)

authors: Gibson, Kerry

abstract: Large numbers of children in New Zealand suffer from mental health problems, and large numbers of children suffer from poverty and hardship. This literature review provides information on the relationship between poverty experienced during childhood and the impact that poverty may have on the mental health of a child or young person, or later in their adulthood. It was found that:
• There is an accepted relationship between poverty experienced in childhood and a greater likelihood of mental health problems through the life span.
• Child poverty and its associated problems such as poor nutrition, inadequate housing, increased likelihood of adverse events and living in poor neighbourhoods put children at higher risk of having mental health problems.
• The evidence strongly suggests that the incidence of mental health conditions among children and adolescents can be reduced by addressing severe and persistent poverty, particularly during the early years of a child’s life.
• Intervention to address poverty and the effects of poverty on children is likely to prevent the perpetuation of inter-generational cycles of poverty and poor mental health.
• The prevalence of child poverty and mental health issues is likely to be higher for Māori and Pasifika than for other children and young people.
• While many Māori and Pasifika children are subject to inequities in material and socio-economic circumstances as well as institutional racism, they also experience the benefits of a rich cultural life and sense of belonging that is seldom accounted for in research reports that focus on deprivation.
The evidence strongly suggests that the incidence of mental health problems throughout the lifespan could be reduced through addressing the causes of child poverty and associated factors. Any mental health strategy for children should sit alongside a comprehensive programme to alleviate poverty.
Strategies aimed at addressing child poverty in Māori and Pasifika communities are more likely to be effective if these are well-resourced at an early stage and developed in a genuine partnership with local communities.

text: Child poverty and mental health: A literature review (Commissioned for New Zealand Psychological Society and Child Poverty Action Group) Large numbers of children in New Zealand suffer from mental health problems, and large numbers of children suffer from poverty and hardship. This literature review provides information on the relationship between poverty experienced during childhood and the impact that poverty may have on the mental health of a child or young person, or later in their adulthood. It was found that:
• There is an accepted relationship between poverty experienced in childhood and a greater likelihood of mental health problems through the life span.
• Child poverty and its associated problems such as poor nutrition, inadequate housing, increased likelihood of adverse events and living in poor neighbourhoods put children at higher risk of having mental health problems.
• The evidence strongly suggests that the incidence of mental health conditions among children and adolescents can be reduced by addressing severe and persistent poverty, particularly during the early years of a child’s life.
• Intervention to address poverty and the effects of poverty on children is likely to prevent the perpetuation of inter-generational cycles of poverty and poor mental health.
• The prevalence of child poverty and mental health issues is likely to be higher for Māori and Pasifika than for other children and young people.
• While many Māori and Pasifika children are subject to inequities in material and socio-economic circumstances as well as institutional racism, they also experience the benefits of a rich cultural life and sense of belonging that is seldom accounted for in research reports that focus on deprivation.
The evidence strongly suggests that the incidence of mental health problems throughout the lifespan could be reduced through addressing the causes of child poverty and associated factors. Any mental health strategy for children should sit alongside a comprehensive programme to alleviate poverty.
Strategies aimed at addressing child poverty in Māori and Pasifika communities are more likely to be effective if these are well-resourced at an early stage and developed in a genuine partnership with local communities.

year: 2017