title: A Comparison of Equine Adipose Tissue, Bone Marrow and Peripheral Blood as Sources of Mesenchymal Stem Cells

authors: Redmond Hubbard, Amy Louise

subjects: Mesenchymal

abstract: Lameness is a significant cause of wastage in equine athletes, with reportedly 66% days lost from training being contributed to musculoskeletal injuries. The use of regenerative cell therapies has been suggested as an alternative to current treatments which have low tissue restorative success. Although stem cell treatments are currently utilised, the optimal tissue source of equine mesenchymal stem cells (MSC) for treatment has not been determined.

The aim of the project was to compare the recovery of MSCs from adipose tissue (AT), bone marrow (BM) and peripheral blood (pB). Stem cell characteristics including adherence, proliferation, multipotency, as well as gene expression for stemness and differentiation markers were investigated. 

Adipose tissue, bone marrow and peripheral blood samples were isolated from six mares and grown to confluence to investigate MSC yield and proliferation rate. The samples were cryopreserved in liquid nitrogen and then placed into a trilineage differentiation assay to assess tissue-dependent plasticity. Messenger RNA was isolated before and during differentiation to assess changes in gene expression. 

All three tissue sources yielded fibroblast MSC-like cells however, AT and BM were found to be superior source of MSCs compared to pB. Trilineage differentiation capacity was demonstrated in AT and BM derived MSCs while peripheral blood derived stem cells were not investigated for multipotency due to a low number of samples. The selected pluripotent ‘stemness’ markers were not widely expressed in any of the three cell types and may not be suitable for use with multipotent stem cells of equine origin. Bone marrow derived stem cells displayed superior expression of differentiation markers compared to adipose derived stem cells.

Adipose tissue was found to be a rich reliable source of MSCs however, this cell source displayed lower efficiency for chondrogenic differentiation. Overall, bone marrow derived stem cells appeared to be a superior source of equine MSCs. An in vivo investigation of treating musculoskeletal injuries with BM and AT derived MSCs is required to determine which source of MSCs offers the best therapeutic benefit for injured horses.

text: A Comparison of Equine Adipose Tissue, Bone Marrow and Peripheral Blood as Sources of Mesenchymal Stem Cells Lameness is a significant cause of wastage in equine athletes, with reportedly 66% days lost from training being contributed to musculoskeletal injuries. The use of regenerative cell therapies has been suggested as an alternative to current treatments which have low tissue restorative success. Although stem cell treatments are currently utilised, the optimal tissue source of equine mesenchymal stem cells (MSC) for treatment has not been determined.

The aim of the project was to compare the recovery of MSCs from adipose tissue (AT), bone marrow (BM) and peripheral blood (pB). Stem cell characteristics including adherence, proliferation, multipotency, as well as gene expression for stemness and differentiation markers were investigated. 

Adipose tissue, bone marrow and peripheral blood samples were isolated from six mares and grown to confluence to investigate MSC yield and proliferation rate. The samples were cryopreserved in liquid nitrogen and then placed into a trilineage differentiation assay to assess tissue-dependent plasticity. Messenger RNA was isolated before and during differentiation to assess changes in gene expression. 

All three tissue sources yielded fibroblast MSC-like cells however, AT and BM were found to be superior source of MSCs compared to pB. Trilineage differentiation capacity was demonstrated in AT and BM derived MSCs while peripheral blood derived stem cells were not investigated for multipotency due to a low number of samples. The selected pluripotent ‘stemness’ markers were not widely expressed in any of the three cell types and may not be suitable for use with multipotent stem cells of equine origin. Bone marrow derived stem cells displayed superior expression of differentiation markers compared to adipose derived stem cells.

Adipose tissue was found to be a rich reliable source of MSCs however, this cell source displayed lower efficiency for chondrogenic differentiation. Overall, bone marrow derived stem cells appeared to be a superior source of equine MSCs. An in vivo investigation of treating musculoskeletal injuries with BM and AT derived MSCs is required to determine which source of MSCs offers the best therapeutic benefit for injured horses.

year: 2014

title: Fabrication of blended chitosan nanofibers by the free surface wire electrospinning

authors: AlJbour, Nawzat D.

abstract: Nanofibers are fibers with diameters in the nanometer range. They are characterized by their high surface area to volume ratio, flexible surface functionalities, and superior mechanical properties. Chitosan is a polycationic polymer which is abundant in nature. Chitosan nanofibers have been widely explored for different potential applications such as wound dressing, tissue engineering, and drug delivery systems. It is difficult to directly spin pure chitosan, due to its high molecular weight, low solubility, and high viscosity. To produce nanofibers, chitosan is commonly blended with other polymers that possess fiber forming capabilities such as polyvinyl alcohol, polycaprolactone, and Polylactic acid. In this study chitosan oligomers of an average molecular weight 15 kDa was blended with the three copolymers at different weight ratios. The fibers were prepared by the free surface wire electrospinning process, and the formed Chitosan nanofibers were characterized by Scanning Electron Microscopy (SEM). SEM results showed that blending chitosan with PLA enhances its spinnability and facilitates uniform and smooth morphology. Blending chitosan with PLA produced nanofibers with better quality, compared to PVA, and PCL.

text: Fabrication of blended chitosan nanofibers by the free surface wire electrospinning Nanofibers are fibers with diameters in the nanometer range. They are characterized by their high surface area to volume ratio, flexible surface functionalities, and superior mechanical properties. Chitosan is a polycationic polymer which is abundant in nature. Chitosan nanofibers have been widely explored for different potential applications such as wound dressing, tissue engineering, and drug delivery systems. It is difficult to directly spin pure chitosan, due to its high molecular weight, low solubility, and high viscosity. To produce nanofibers, chitosan is commonly blended with other polymers that possess fiber forming capabilities such as polyvinyl alcohol, polycaprolactone, and Polylactic acid. In this study chitosan oligomers of an average molecular weight 15 kDa was blended with the three copolymers at different weight ratios. The fibers were prepared by the free surface wire electrospinning process, and the formed Chitosan nanofibers were characterized by Scanning Electron Microscopy (SEM). SEM results showed that blending chitosan with PLA enhances its spinnability and facilitates uniform and smooth morphology. Blending chitosan with PLA produced nanofibers with better quality, compared to PVA, and PCL.

year: 2023-01-01

title: Te whakaako i te reo Māori i te kura auraki tuarua i Aotearoa nei: Kei tua o te awe māpere. The teaching of te reo Māori in English-medium secondary schools in New Zealand: Beyond the mask.

authors: Nock, Sophie

subjects: language curricula

abstract: He taonga te reo Māori nō tuawhakarere, nō tuauriuri, whāioio, nā te atua i homai kia poipoia e te iwi Māori hei reo kōrero mō te hunga kaingākau, mō te hunga kāore anō kia whānau noa.  Ahakoa ia he toimaha tonu te hauora o te reo Māori, nā te mahi tūkino (a ētahi).  I roto i ngā tau whā tekau kua ara ake ngā momo rongoā hei whakahauora, hei whakatairanga i te reo Māori, nā, ko Te Kōhanga Reo tērā, ko te Kura Kaupapa Māori tērā, ko Te Wharekura tērā, aha atu, aha atu.  Ka mutu, i te tau 1987 i whakamanahia ai te reo Māori hei reo tūturu o tēnei whenua.  Ahakoa he aha e mea ana te Tāhūhū o te Mātauranga ehara i te mea me whai wāhi te reo Māori hei reo ako i roto i ngā kura tuarua puta noa i te motu.  Engari, ko te raruraru kua whai wāhi kē ētahi atu reo o te ao, kaua ko te reo Māori.  Kāti, i te tau 2009 i tatū ai Te Aho Arataki Marau mō te Ako i Te Reo Māori, ā, ki reira whai wāhi ai te reo Māori.  Nā, ko tā te kura tuarua mahi he tautoko mārika i te reo Māori, mā te whakaako i te reo Māori, e tika ana me pērā, nā te mea, ko te nuinga o ngā tamariki Māori e haere ana ki a ia ako ai.  Mōhio pai i whakapeto ngoi ngā kaiwhakaako reo Māori me te matatau anō o ētahi ki te reo hoki, engari, ki tā te pūrongo rangahau a Te Tari Arotake Mātauranga kīhai i tino eke panuku ngā mahi whakaako.  Ko ngā tino take, ko te whakangungu kaiwhakaako, ko te tautoko me te whakahaere a te kura hoki.

Nō reira, ko te whāinga matua o tēnei rangahau kia tirohia me pēhea e whakaako ai te reo Māori ki te kura tuarua, (kaua i ngā wharekura) i Aotearoa.  Mai i te rau tau tekau mā waru ka tātaria ētahi o ngā kōrero whanake e hāngai pū ana ki te whakaako me te ako i (t)ētahi atu reo, ko ia te wāhanga tuarua.  Whai muri mai i tērā (kei te wāhanga tuatoru) ko ngā patapatai me ngā uiuitanga, ā, ko tā te patapatai he kukume mai i ngā whakapono, i ngā whakaaro, i ngā waiaro o te hunga kaiwhakaako, ko tā te uiuitanga e whakatātūtū i te rētotanga o te kaupapa.  Ka mutu, i whārikihia ngā whakakitenga, ngā whakahoki kōrero ki taua wāhanga.  I te mutunga iho i whakapau kaha ngā kaiwhakaako ki te whakatutuki i ngā mahi, ahakoa ngā piki me ngā heke.  Kei ngā wāhanga e rua (4 & 5) ngā kōrero mō ngā tūmomo rauemi e whakamahia ana e ngā kaiwhakaako, arā, ko ngā momo pukapuka (4) me ngā tūmomo rauemi o te ipurangi nā Te Tāhūhū o te Mātauranga i whakarato (5).  E kī ana ngā whakaputanga, tuatahi, kīhai aua rauemi i tino whakaatu mai i ngā momo whanaketanga i roto i ngā tau whā tekau neke atu mō te whakaako i te reo tuarua, hou rānei, tuarua, kāhore hoki i tino hāngai pū ēnei rauemi ki te Marau mō te Ako i Te Reo Māori i te kura tuarua me te hāngai anō hoki ki te āhua o te whakaako i te reo mā te ‘Communicative Language Teaching’.  Hei whaiwhai ake i ngā rauemi i tātaria ētahi o ngā karaehe reo (te wāhanga 6) mā ngā wehewehetanga matua o te whakaako reo.  Mai i taua tātaritanga i kitea mai ētahi raruraru, tuatahi, kāhore ngā tauira/akonga i whai wāhi ki te kōrero (i roto tonu i te karaehe), tuarua, kāhore e taea te whakawhitiwhiti kōrero ki a rātou anō, tuatoru, kāhore rātou i te mahi tahi anō hoki.  Hei whakakapi ake i tēnei rangahau, kei te wāhanga whakamutunga (7) te whakakaokao mai o ngā whakakitenga katoa, me te whakatakoto o ngā tūtohutanga kia anga whakamua ai. 

It has been acknowledged that te reo Māori is a taonga and, as such, is subject to the protections guaranteed under the terms of Te Tiriti o Waitangi.  Since 1987, it has been an official language of Aotearoa.  Its future is, however, very far from secure and instructed language learning continues to play a significant part in the revitalisation agenda.  Even so, although schools are now required to offer a language other than the language of instruction to pupils in Years 7 to 10, and although over two thirds of Māori students currently attend schools in which English is the main language of instruction, schools are not required to include te reo Māori in their language offering.  In addition, although curriculum guidelines documents for a number of foreign languages have been available for many years, it was not until 2009 that the final version of a curriculum guidelines document for the teaching and learning of te reo Māori in English-medium schools became available.  Furthermore, while there clearly are some extremely competent teachers of the language, Education Review Office (ERO) reports indicate that all is not well so far as the teaching and learning of te reo Māori in English-medium secondary schools is concerned.  The problems identified relate not to teachers' proficiency in the language but to their limited pedagogical knowledge.  As ERO has indicated, this raises questions about the initial training of teachers of te reo Māori and the ways in which all schools manage and support them. 

The overall aim of the research reported here is to investigate the teaching of te reo Māori in English-medium secondary schools in Aotearoa.  Following a critical review of selected literature on developments in the teaching and learning of additional languages since the end of the 18th Century (Chapter 2), there is a report on a survey (involving questionnaire responses and semi-structured interviews) of a sample of teachers of te reo Māori in English-medium secondary schools.  That survey focuses on their educational and linguistic backgrounds and their attitudes and approaches to a range of issues associated with the teaching and learning of the language in school settings (Chapter 3).  Overall, the findings indicate that while these teachers are doing their best to provide a high quality educational experience for their students, they are doing so in the face of a number of significant barriers relating, in particular, to the limited nature of the pre-service training they have received, and the range of additional duties they are expected to perform.  In addition, the survey revealed some concerns about the impact of national assessment on teaching and learning and about the teaching materials and resources that are available.  The next two chapters report on the analysis of a sample of textbooks which are widely used in secondary schools (Chapter 4) and a sample of resources made available by the Ministry of Education (Chapter 5), the main findings being that these are, in the main, (a) inconsistent with both research-based developments in the teaching of additional languages and the expectations relating to communicative language teaching as outlined in the curriculum guidelines, and (b) that these resources do not align with the new curriculum.  The analysis of a sample of lessons taught in secondary schools follows (Chapter 6).  That analysis, conducted in relation to a number of focus points, reveals some significant problems, including the fact that the students were provided with few opportunities to contribute, to interact with one another, and to engage in genuinely communicative activities.  The final chapter (Chapter 7) provides an overview of the findings and includes some suggestions about a possible way forward.

text: Te whakaako i te reo Māori i te kura auraki tuarua i Aotearoa nei: Kei tua o te awe māpere. The teaching of te reo Māori in English-medium secondary schools in New Zealand: Beyond the mask. He taonga te reo Māori nō tuawhakarere, nō tuauriuri, whāioio, nā te atua i homai kia poipoia e te iwi Māori hei reo kōrero mō te hunga kaingākau, mō te hunga kāore anō kia whānau noa.  Ahakoa ia he toimaha tonu te hauora o te reo Māori, nā te mahi tūkino (a ētahi).  I roto i ngā tau whā tekau kua ara ake ngā momo rongoā hei whakahauora, hei whakatairanga i te reo Māori, nā, ko Te Kōhanga Reo tērā, ko te Kura Kaupapa Māori tērā, ko Te Wharekura tērā, aha atu, aha atu.  Ka mutu, i te tau 1987 i whakamanahia ai te reo Māori hei reo tūturu o tēnei whenua.  Ahakoa he aha e mea ana te Tāhūhū o te Mātauranga ehara i te mea me whai wāhi te reo Māori hei reo ako i roto i ngā kura tuarua puta noa i te motu.  Engari, ko te raruraru kua whai wāhi kē ētahi atu reo o te ao, kaua ko te reo Māori.  Kāti, i te tau 2009 i tatū ai Te Aho Arataki Marau mō te Ako i Te Reo Māori, ā, ki reira whai wāhi ai te reo Māori.  Nā, ko tā te kura tuarua mahi he tautoko mārika i te reo Māori, mā te whakaako i te reo Māori, e tika ana me pērā, nā te mea, ko te nuinga o ngā tamariki Māori e haere ana ki a ia ako ai.  Mōhio pai i whakapeto ngoi ngā kaiwhakaako reo Māori me te matatau anō o ētahi ki te reo hoki, engari, ki tā te pūrongo rangahau a Te Tari Arotake Mātauranga kīhai i tino eke panuku ngā mahi whakaako.  Ko ngā tino take, ko te whakangungu kaiwhakaako, ko te tautoko me te whakahaere a te kura hoki.

Nō reira, ko te whāinga matua o tēnei rangahau kia tirohia me pēhea e whakaako ai te reo Māori ki te kura tuarua, (kaua i ngā wharekura) i Aotearoa.  Mai i te rau tau tekau mā waru ka tātaria ētahi o ngā kōrero whanake e hāngai pū ana ki te whakaako me te ako i (t)ētahi atu reo, ko ia te wāhanga tuarua.  Whai muri mai i tērā (kei te wāhanga tuatoru) ko ngā patapatai me ngā uiuitanga, ā, ko tā te patapatai he kukume mai i ngā whakapono, i ngā whakaaro, i ngā waiaro o te hunga kaiwhakaako, ko tā te uiuitanga e whakatātūtū i te rētotanga o te kaupapa.  Ka mutu, i whārikihia ngā whakakitenga, ngā whakahoki kōrero ki taua wāhanga.  I te mutunga iho i whakapau kaha ngā kaiwhakaako ki te whakatutuki i ngā mahi, ahakoa ngā piki me ngā heke.  Kei ngā wāhanga e rua (4 & 5) ngā kōrero mō ngā tūmomo rauemi e whakamahia ana e ngā kaiwhakaako, arā, ko ngā momo pukapuka (4) me ngā tūmomo rauemi o te ipurangi nā Te Tāhūhū o te Mātauranga i whakarato (5).  E kī ana ngā whakaputanga, tuatahi, kīhai aua rauemi i tino whakaatu mai i ngā momo whanaketanga i roto i ngā tau whā tekau neke atu mō te whakaako i te reo tuarua, hou rānei, tuarua, kāhore hoki i tino hāngai pū ēnei rauemi ki te Marau mō te Ako i Te Reo Māori i te kura tuarua me te hāngai anō hoki ki te āhua o te whakaako i te reo mā te ‘Communicative Language Teaching’.  Hei whaiwhai ake i ngā rauemi i tātaria ētahi o ngā karaehe reo (te wāhanga 6) mā ngā wehewehetanga matua o te whakaako reo.  Mai i taua tātaritanga i kitea mai ētahi raruraru, tuatahi, kāhore ngā tauira/akonga i whai wāhi ki te kōrero (i roto tonu i te karaehe), tuarua, kāhore e taea te whakawhitiwhiti kōrero ki a rātou anō, tuatoru, kāhore rātou i te mahi tahi anō hoki.  Hei whakakapi ake i tēnei rangahau, kei te wāhanga whakamutunga (7) te whakakaokao mai o ngā whakakitenga katoa, me te whakatakoto o ngā tūtohutanga kia anga whakamua ai. 

It has been acknowledged that te reo Māori is a taonga and, as such, is subject to the protections guaranteed under the terms of Te Tiriti o Waitangi.  Since 1987, it has been an official language of Aotearoa.  Its future is, however, very far from secure and instructed language learning continues to play a significant part in the revitalisation agenda.  Even so, although schools are now required to offer a language other than the language of instruction to pupils in Years 7 to 10, and although over two thirds of Māori students currently attend schools in which English is the main language of instruction, schools are not required to include te reo Māori in their language offering.  In addition, although curriculum guidelines documents for a number of foreign languages have been available for many years, it was not until 2009 that the final version of a curriculum guidelines document for the teaching and learning of te reo Māori in English-medium schools became available.  Furthermore, while there clearly are some extremely competent teachers of the language, Education Review Office (ERO) reports indicate that all is not well so far as the teaching and learning of te reo Māori in English-medium secondary schools is concerned.  The problems identified relate not to teachers' proficiency in the language but to their limited pedagogical knowledge.  As ERO has indicated, this raises questions about the initial training of teachers of te reo Māori and the ways in which all schools manage and support them. 

The overall aim of the research reported here is to investigate the teaching of te reo Māori in English-medium secondary schools in Aotearoa.  Following a critical review of selected literature on developments in the teaching and learning of additional languages since the end of the 18th Century (Chapter 2), there is a report on a survey (involving questionnaire responses and semi-structured interviews) of a sample of teachers of te reo Māori in English-medium secondary schools.  That survey focuses on their educational and linguistic backgrounds and their attitudes and approaches to a range of issues associated with the teaching and learning of the language in school settings (Chapter 3).  Overall, the findings indicate that while these teachers are doing their best to provide a high quality educational experience for their students, they are doing so in the face of a number of significant barriers relating, in particular, to the limited nature of the pre-service training they have received, and the range of additional duties they are expected to perform.  In addition, the survey revealed some concerns about the impact of national assessment on teaching and learning and about the teaching materials and resources that are available.  The next two chapters report on the analysis of a sample of textbooks which are widely used in secondary schools (Chapter 4) and a sample of resources made available by the Ministry of Education (Chapter 5), the main findings being that these are, in the main, (a) inconsistent with both research-based developments in the teaching of additional languages and the expectations relating to communicative language teaching as outlined in the curriculum guidelines, and (b) that these resources do not align with the new curriculum.  The analysis of a sample of lessons taught in secondary schools follows (Chapter 6).  That analysis, conducted in relation to a number of focus points, reveals some significant problems, including the fact that the students were provided with few opportunities to contribute, to interact with one another, and to engage in genuinely communicative activities.  The final chapter (Chapter 7) provides an overview of the findings and includes some suggestions about a possible way forward.

year: 2014