title: Effects of copper sprays on microbial communities in kiwifruit orchard soils

authors: Dean, Fiona Patricia

abstract: There has been a significant increase in the use of antimicrobial copper sprays on kiwifruit orchards in the Western Bay of Plenty (WBOP) since the first outbreak of the bacterial vine disease Pseudomonas syringae pv. actinidiae (Psa-V) in 2010. Studies have revealed that an accumulation of copper in soil may adversely affect soil microorganisms, which are pivotal in essential soil functions such as nutrient assimilation.
The aim of this study was to determine whether or not the use of copper sprays on WBOP kiwifruit orchards has had detrimental effects on soil microorganisms and if this has resulted in changes to the structure of soil microbiological communities.
Soil was sampled from ten WBOP kiwifruit orchards with varying levels of copper but with similar physicochemical properties. These included five sets of two adjacent orchards, of which one was organic and the other conventional, and for which data was available on soil copper levels for 2011. 
DNA extracted from each soil was amplified with PCR and sequenced using Ion Torrent Sequencing technology. The resulting bacterial and archaeal sequences were binned into operational taxonomic units (OTUs) and the phylogeny of each was determined using the Ribosomal Database Project (RDP) Classifier. Soils were also analysed for a number of physicochemical properties including total, bioavailable and free ionic copper.
Non-metric Multidimensional Scaling (NMDS) and Multi-Response Permutation Procedures (MRPP) were used to examine dissimilarities between microbial communities in relation to soil copper levels, management practices and other physicochemical variables. Indicator species analyses were conducted on OTU abundance data to determine if particular OTUs were indicative of soils with the lowest and highest copper levels. Community data were also compared using a number of measures of diversity. Differences in physicochemical parameters between soils were analysed for significance using ANOVA and t-tests.
Results revealed that levels of total copper in soils have increased since 2011, with significant differences measured between each pair of orchards. Significant differences in soil microbial communities were also revealed, with pH identified as the main driver of community composition within and between orchards and differences between orchards were also largely explained by management practices. Relative abundances of Archaea were significantly higher in conventional orchards and in soils with the highest levels of copper. However, overall community dissimilarities were not found to be related to soil copper levels. Indicator species analysis revealed that soils with < 30 mg kg-1 of total copper had a greater abundance of Actinobacteria, whilst soils with > 60 mg kg-1 had a higher representation of Chlamydiae, Chloroflexi, and Thaumarchaeota. 
The conclusions of this research are that current copper levels in the study orchard soils have not significantly influenced soil microbial community composition and associated functions. However, the high number of influential variables confounded attempts at identifying differences due to copper alone. In addition, the lack of knowledge on the specific functions of individual taxa, make it very difficult to reveal the long-term implications of even subtle differences in community composition as a result of copper use.

text: Effects of copper sprays on microbial communities in kiwifruit orchard soils There has been a significant increase in the use of antimicrobial copper sprays on kiwifruit orchards in the Western Bay of Plenty (WBOP) since the first outbreak of the bacterial vine disease Pseudomonas syringae pv. actinidiae (Psa-V) in 2010. Studies have revealed that an accumulation of copper in soil may adversely affect soil microorganisms, which are pivotal in essential soil functions such as nutrient assimilation.
The aim of this study was to determine whether or not the use of copper sprays on WBOP kiwifruit orchards has had detrimental effects on soil microorganisms and if this has resulted in changes to the structure of soil microbiological communities.
Soil was sampled from ten WBOP kiwifruit orchards with varying levels of copper but with similar physicochemical properties. These included five sets of two adjacent orchards, of which one was organic and the other conventional, and for which data was available on soil copper levels for 2011. 
DNA extracted from each soil was amplified with PCR and sequenced using Ion Torrent Sequencing technology. The resulting bacterial and archaeal sequences were binned into operational taxonomic units (OTUs) and the phylogeny of each was determined using the Ribosomal Database Project (RDP) Classifier. Soils were also analysed for a number of physicochemical properties including total, bioavailable and free ionic copper.
Non-metric Multidimensional Scaling (NMDS) and Multi-Response Permutation Procedures (MRPP) were used to examine dissimilarities between microbial communities in relation to soil copper levels, management practices and other physicochemical variables. Indicator species analyses were conducted on OTU abundance data to determine if particular OTUs were indicative of soils with the lowest and highest copper levels. Community data were also compared using a number of measures of diversity. Differences in physicochemical parameters between soils were analysed for significance using ANOVA and t-tests.
Results revealed that levels of total copper in soils have increased since 2011, with significant differences measured between each pair of orchards. Significant differences in soil microbial communities were also revealed, with pH identified as the main driver of community composition within and between orchards and differences between orchards were also largely explained by management practices. Relative abundances of Archaea were significantly higher in conventional orchards and in soils with the highest levels of copper. However, overall community dissimilarities were not found to be related to soil copper levels. Indicator species analysis revealed that soils with < 30 mg kg-1 of total copper had a greater abundance of Actinobacteria, whilst soils with > 60 mg kg-1 had a higher representation of Chlamydiae, Chloroflexi, and Thaumarchaeota. 
The conclusions of this research are that current copper levels in the study orchard soils have not significantly influenced soil microbial community composition and associated functions. However, the high number of influential variables confounded attempts at identifying differences due to copper alone. In addition, the lack of knowledge on the specific functions of individual taxa, make it very difficult to reveal the long-term implications of even subtle differences in community composition as a result of copper use.

year: 2016

title: Citizenship, democracy and social justice: A conversation with Maria Olson

authors: Peters, Michael A.

abstract: Maria Olson is a researcher and lecturer in Education at Stockholm University and the University of Skövde, Sweden. Her areas of interest include democracy and citizenship in relation to education. Her major fields are educational theory and educational philosophy. Her current publications include most recently a series of papers that develop themes of citizenship, democracy and social justice, including: “Citizenship Education without Citizenship? The Migrant in EU Policy on Participatory Citizenship – Toward the Margin through ‘Strangification,’” in R. Hedke and T. Zimenkova (eds.), Education for Civic and Political Participation: A Critical Approach (pp. 155–170). London: Routledge, 2012; “Citizenship ‘in Between’: The Local and the Global Scope of European Citizenship in Swedish Educational Policy,” in S. Goncales and M. A. Carpenter (eds.), Intercultural Policies and Education (pp. 193–203). New York: Peter Lang, 2012; “The European ‘We’: From Citizenship Policy to the Role of Education,” Studies in Philosophy and Education 31(1), 77–89, 2012; “Opening Discourses of Citizenship Education: Theorizing with Foucault” (with Nicoll, K., Fejes, A., Dahlstedt, M. & Biesta, G. J. J.), Journal of Education Policy, 2013 (forthcoming); “Democracy Lessons in Market-oriented Schools: The Case of Swedish Upper Secondary Education,” Education, Citizenship and Social Justice, online first, Doi: 10.1177/17461979134836842013 (with Lundahl, Lisbeth), 2013; “What Counts as Young People’s Civic Engagement in Times of Accountability? On the Importance of Maintaining Openness about Young People’s Civic Engagement in Education,” in M. Olson (ed.), Theme: Citizenship Education under Liberal Democracy. Utbildning & Demokrati [Education & Democracy] 21(1), 29–55, 2012.

text: Citizenship, democracy and social justice: A conversation with Maria Olson Maria Olson is a researcher and lecturer in Education at Stockholm University and the University of Skövde, Sweden. Her areas of interest include democracy and citizenship in relation to education. Her major fields are educational theory and educational philosophy. Her current publications include most recently a series of papers that develop themes of citizenship, democracy and social justice, including: “Citizenship Education without Citizenship? The Migrant in EU Policy on Participatory Citizenship – Toward the Margin through ‘Strangification,’” in R. Hedke and T. Zimenkova (eds.), Education for Civic and Political Participation: A Critical Approach (pp. 155–170). London: Routledge, 2012; “Citizenship ‘in Between’: The Local and the Global Scope of European Citizenship in Swedish Educational Policy,” in S. Goncales and M. A. Carpenter (eds.), Intercultural Policies and Education (pp. 193–203). New York: Peter Lang, 2012; “The European ‘We’: From Citizenship Policy to the Role of Education,” Studies in Philosophy and Education 31(1), 77–89, 2012; “Opening Discourses of Citizenship Education: Theorizing with Foucault” (with Nicoll, K., Fejes, A., Dahlstedt, M. & Biesta, G. J. J.), Journal of Education Policy, 2013 (forthcoming); “Democracy Lessons in Market-oriented Schools: The Case of Swedish Upper Secondary Education,” Education, Citizenship and Social Justice, online first, Doi: 10.1177/17461979134836842013 (with Lundahl, Lisbeth), 2013; “What Counts as Young People’s Civic Engagement in Times of Accountability? On the Importance of Maintaining Openness about Young People’s Civic Engagement in Education,” in M. Olson (ed.), Theme: Citizenship Education under Liberal Democracy. Utbildning & Demokrati [Education & Democracy] 21(1), 29–55, 2012.

year: 2013

title: 'The voices caused him to become porangi': Maori Patients in the Auckland Lunatic Asylum, 1860-1900

authors: Burke, Lorelle J.

abstract: Histories of the asylum have raised a number of important questions about the way 'race' intersects along colonial, medical and legal boundaries. The experience of Maori patients at the Auckland Lunatic Asylum was a site where colonial attitudes towards 'race' were played out along such lines. By employing 'race' as a category of historical analysis, one can attempt to investigate how the asylum processes in Aotearoa/New Zealand exemplified the question of 'race' in an effort to make more visible the experiences, attitudes and responses of Maori asylum patients. 
This thesis examines colonial 'madness' by investigating the experiences of Maori patients at the Auckland Lunatic Asylum from 1860-1900. Using patient case records from this period this thesis interrogates the experiences of Maori patients in a colonial asylum, away from their whanau, communities, and tribal networks; what factors contributed to this, and how colonial attitudes towards these patients were acted out. The first chapter therefore, explores the 'medico-legal' management of these Maori patients, followed in Chapter Two by a broad overview of the Maori patients themselves. Chapter Three analyses the case records in more depth illustrating the complex interplay between 'race', gender, 'whiteness' and culture. Chapter Four looks at the way Maori dealt with the insane away from European institutions such as the asylum, emphasising how colonial histories of medicine now paying attention to Indigenous peoples in their context, and to medical pluralism, which reflects European models on one hand and on the other, Maori modes. 
In considering Maori patients, this thesis reflects on the very 'human experience' that can be revealed by using asylum case files, and what such sources may suggest about 'race' as an implicit part of colonial asylum administration. More widely, the paper also reflects on colonisation, Maori and the asylum, and the ways in which Maori used the asylum.

text: 'The voices caused him to become porangi': Maori Patients in the Auckland Lunatic Asylum, 1860-1900 Histories of the asylum have raised a number of important questions about the way 'race' intersects along colonial, medical and legal boundaries. The experience of Maori patients at the Auckland Lunatic Asylum was a site where colonial attitudes towards 'race' were played out along such lines. By employing 'race' as a category of historical analysis, one can attempt to investigate how the asylum processes in Aotearoa/New Zealand exemplified the question of 'race' in an effort to make more visible the experiences, attitudes and responses of Maori asylum patients. 
This thesis examines colonial 'madness' by investigating the experiences of Maori patients at the Auckland Lunatic Asylum from 1860-1900. Using patient case records from this period this thesis interrogates the experiences of Maori patients in a colonial asylum, away from their whanau, communities, and tribal networks; what factors contributed to this, and how colonial attitudes towards these patients were acted out. The first chapter therefore, explores the 'medico-legal' management of these Maori patients, followed in Chapter Two by a broad overview of the Maori patients themselves. Chapter Three analyses the case records in more depth illustrating the complex interplay between 'race', gender, 'whiteness' and culture. Chapter Four looks at the way Maori dealt with the insane away from European institutions such as the asylum, emphasising how colonial histories of medicine now paying attention to Indigenous peoples in their context, and to medical pluralism, which reflects European models on one hand and on the other, Maori modes. 
In considering Maori patients, this thesis reflects on the very 'human experience' that can be revealed by using asylum case files, and what such sources may suggest about 'race' as an implicit part of colonial asylum administration. More widely, the paper also reflects on colonisation, Maori and the asylum, and the ways in which Maori used the asylum.

year: 2006