title: A core phyllosphere microbiome exists across distant populations of a tree species indigenous to New Zealand

authors: Noble, Anya S.

subjects: Science & Technology

abstract: The phyllosphere microbiome is increasingly recognised as an influential component of plant physiology, yet it remains unclear whether stable host-microbe associations generally exist in the phyllosphere. Leptospermum scoparium (mānuka) is a tea tree indigenous to New Zealand, and honey derived from mānuka is widely known to possess unique antimicrobial properties. However, the host physiological traits associated with these antimicrobial properties vary widely, and the specific cause of such variation has eluded scientists despite decades of research. Notably, the mānuka phyllosphere microbiome remains uncharacterised, and its potential role in mediating host physiology has not been considered. Working within the prevailing core microbiome conceptual framework, we hypothesise that the phyllosphere microbiome of mānuka exhibits specific host association patterns congruent with those of a microbial community under host selective pressure (null hypothesis: the mānuka phyllosphere microbiome is recruited stochastically from the surrounding environment). To examine our hypothesis, we characterised the phyllosphere and associated soil microbiomes of five distinct and geographically distant mānuka populations across the North Island of New Zealand. We identified a habitat-specific and relatively abundant core microbiome in the mānuka phyllosphere, which was persistent across all samples. In contrast, non-core phyllosphere microorganisms exhibited significant variation across individual host trees and populations that was strongly driven by environmental and spatial factors. Our results demonstrate the existence of a dominant and ubiquitous core microbiome in the phyllosphere of mānuka, supporting our hypothesis that phyllosphere microorganisms of mānuka exhibit specific host association and potentially mediate physiological traits of this nationally and culturally treasured indigenous plant. In addition, our results illustrate biogeographical patterns in mānuka phyllosphere microbiomes and offer insight into factors contributing to phyllosphere microbiome assembly.

text: A core phyllosphere microbiome exists across distant populations of a tree species indigenous to New Zealand The phyllosphere microbiome is increasingly recognised as an influential component of plant physiology, yet it remains unclear whether stable host-microbe associations generally exist in the phyllosphere. Leptospermum scoparium (mānuka) is a tea tree indigenous to New Zealand, and honey derived from mānuka is widely known to possess unique antimicrobial properties. However, the host physiological traits associated with these antimicrobial properties vary widely, and the specific cause of such variation has eluded scientists despite decades of research. Notably, the mānuka phyllosphere microbiome remains uncharacterised, and its potential role in mediating host physiology has not been considered. Working within the prevailing core microbiome conceptual framework, we hypothesise that the phyllosphere microbiome of mānuka exhibits specific host association patterns congruent with those of a microbial community under host selective pressure (null hypothesis: the mānuka phyllosphere microbiome is recruited stochastically from the surrounding environment). To examine our hypothesis, we characterised the phyllosphere and associated soil microbiomes of five distinct and geographically distant mānuka populations across the North Island of New Zealand. We identified a habitat-specific and relatively abundant core microbiome in the mānuka phyllosphere, which was persistent across all samples. In contrast, non-core phyllosphere microorganisms exhibited significant variation across individual host trees and populations that was strongly driven by environmental and spatial factors. Our results demonstrate the existence of a dominant and ubiquitous core microbiome in the phyllosphere of mānuka, supporting our hypothesis that phyllosphere microorganisms of mānuka exhibit specific host association and potentially mediate physiological traits of this nationally and culturally treasured indigenous plant. In addition, our results illustrate biogeographical patterns in mānuka phyllosphere microbiomes and offer insight into factors contributing to phyllosphere microbiome assembly.

year: 2020

title: Maori women and research: Researching ourselves

authors: Te Awekotuku, Ngahuia

subjects: Maori

abstract: This was the closing keynote address at the Student Symposium organized by the Maori &
Psychology Research Unit at the University of Waikato, Hamilton in August 1999. Most of the
people attending were Maori, and female, and I spoke to, for, and about us. The speech was
transcribed from an oral address with transparencies, and has been revised here for this
publication.

text: Maori women and research: Researching ourselves This was the closing keynote address at the Student Symposium organized by the Maori &
Psychology Research Unit at the University of Waikato, Hamilton in August 1999. Most of the
people attending were Maori, and female, and I spoke to, for, and about us. The speech was
transcribed from an oral address with transparencies, and has been revised here for this
publication.

year: 1999

title: The development and validation of the Successful Psychopathy Scale [Protocol]

authors: Louise, Wallace

abstract: The personality construct known as ‘Successful Psychopathy’ has attracted the interests of researchers and clinicians alike. The concept suggests an individual who demonstrates the core traits associated with psychopathy but is able to adapt and function within society to prototypical or superior standards. There has yet to be a sound theoretical model of this construct by which to base a psychometric measure. This protocol presents the ethical procedure that will endeavour to create such a measure and validate it within general population samples.

text: The development and validation of the Successful Psychopathy Scale [Protocol] The personality construct known as ‘Successful Psychopathy’ has attracted the interests of researchers and clinicians alike. The concept suggests an individual who demonstrates the core traits associated with psychopathy but is able to adapt and function within society to prototypical or superior standards. There has yet to be a sound theoretical model of this construct by which to base a psychometric measure. This protocol presents the ethical procedure that will endeavour to create such a measure and validate it within general population samples.

year: 2021