title: Fluorescent probes of the orientation of myosin regulatory light chains in relaxed, rigor, and contracting muscle.

authors: Ling, Nicholas

abstract: The orientation of the light-chain region of myosin heads in relaxed, rigor, and isometrically contracting fibers from rabbit psoas muscle was studied by fluorescence polarization. Cysteine 108 of chicken gizzard myosin regulatory light chain (cgRLC) was covalently modified with iodoacetamidotetramethylrhodamine (iodo-ATR). Native RLC of single glycerinated muscle fibers was exchanged for labeled cgRLC in a low [Mg2+] rigor solution at 30 degrees C. Troponin and troponin C removed in this procedure were replaced. RLC exchange had little effect on active force production. X-ray diffraction showed normal structure in rigor after RLC exchange, but loss of axial and helical order in relaxation. In isolated myofibrils labeled cgRLC was confined to the regions of the sarcomere containing myosin heads. The ATR dipoles showed a preference for orientations perpendicular to the fiber axis, combined with limited nanosecond rotational motion, in all conditions studied. The perpendicular orientation preference was more marked in rigor than in either relaxation or active contraction. Stretching relaxed fibers to sarcomere length 4 microns to eliminate overlap between actin- and myosin-containing filaments had little effect on the orientation preference. There was no change in orientation preference when fibers were put into rigor at sarcomere length 4.0 microns. Qualitatively similar results were obtained with ATR-labeled rabbit skeletal RLC.

text: Fluorescent probes of the orientation of myosin regulatory light chains in relaxed, rigor, and contracting muscle. The orientation of the light-chain region of myosin heads in relaxed, rigor, and isometrically contracting fibers from rabbit psoas muscle was studied by fluorescence polarization. Cysteine 108 of chicken gizzard myosin regulatory light chain (cgRLC) was covalently modified with iodoacetamidotetramethylrhodamine (iodo-ATR). Native RLC of single glycerinated muscle fibers was exchanged for labeled cgRLC in a low [Mg2+] rigor solution at 30 degrees C. Troponin and troponin C removed in this procedure were replaced. RLC exchange had little effect on active force production. X-ray diffraction showed normal structure in rigor after RLC exchange, but loss of axial and helical order in relaxation. In isolated myofibrils labeled cgRLC was confined to the regions of the sarcomere containing myosin heads. The ATR dipoles showed a preference for orientations perpendicular to the fiber axis, combined with limited nanosecond rotational motion, in all conditions studied. The perpendicular orientation preference was more marked in rigor than in either relaxation or active contraction. Stretching relaxed fibers to sarcomere length 4 microns to eliminate overlap between actin- and myosin-containing filaments had little effect on the orientation preference. There was no change in orientation preference when fibers were put into rigor at sarcomere length 4.0 microns. Qualitatively similar results were obtained with ATR-labeled rabbit skeletal RLC.

year: 1996-04

title: International trade agreements and international migration

authors: Poot, Jacques

subjects: international trade agreement

abstract: Despite large potential economic gains, bilateral and multilateral negotiations focusing on liberalisation of migration have not shared the high profile of international trade negotiations and agreements. Migration and trade have been traditionally viewed rather separately and the relevance of the many, and complex, interdependencies has been given remarkably little attention in the literature to date. In this article, we focus on the two-way interaction between international migration and agreements designed to enhance cross-border trade and investment. Liberalisation of international trade in services and in the movement of people potentially offers much greater economic gains than liberalisation of remaining barriers to goods trade. However, progress within multilateral frameworks is fraught with difficulty. The World Trade Organization’s General Agreement on Trade in Services (GATS) has yielded little real progress so far and negotiations within more flexible unilateral and bilateral frameworks are likely to be more successful in liberalising the movement of labour. We discuss a range of specific examples, focusing particularly on the interesting case of New Zealand. We find that trade agreements are increasingly including agreements on migration, though typically favouring temporary migration and involving numerically modest quotas. We conclude that migration regulatory frameworks are likely to be further and more strongly linked to trade and investment agreements in the future, particularly given changing economic and demographic forces. The primary focus of migration policies may nonetheless remain different from that of trade policies. While further migration liberalisation is likely to be through bilateral and regional agreements, it will be important to try to lock in the gains of such agreements, while simultaneously working to consolidating these in a way that will help to facilitate future multilateral agreement.

text: International trade agreements and international migration Despite large potential economic gains, bilateral and multilateral negotiations focusing on liberalisation of migration have not shared the high profile of international trade negotiations and agreements. Migration and trade have been traditionally viewed rather separately and the relevance of the many, and complex, interdependencies has been given remarkably little attention in the literature to date. In this article, we focus on the two-way interaction between international migration and agreements designed to enhance cross-border trade and investment. Liberalisation of international trade in services and in the movement of people potentially offers much greater economic gains than liberalisation of remaining barriers to goods trade. However, progress within multilateral frameworks is fraught with difficulty. The World Trade Organization’s General Agreement on Trade in Services (GATS) has yielded little real progress so far and negotiations within more flexible unilateral and bilateral frameworks are likely to be more successful in liberalising the movement of labour. We discuss a range of specific examples, focusing particularly on the interesting case of New Zealand. We find that trade agreements are increasingly including agreements on migration, though typically favouring temporary migration and involving numerically modest quotas. We conclude that migration regulatory frameworks are likely to be further and more strongly linked to trade and investment agreements in the future, particularly given changing economic and demographic forces. The primary focus of migration policies may nonetheless remain different from that of trade policies. While further migration liberalisation is likely to be through bilateral and regional agreements, it will be important to try to lock in the gains of such agreements, while simultaneously working to consolidating these in a way that will help to facilitate future multilateral agreement.

year: 2010

title: Kia kāpuia te reo o Tāmaki ki te Tonga: Tāmaki Makaurau ki te Tonga

authors: Lee-Morgan, Jenny Bol Jun

abstract: Mai i te Papakuratanga o Mataoho tae noa ki tona Ipu. Mai i Pukaki Tapu o Poutiikeka ki Te Puketapapatanga a Hape. Mai i te tahuna o Torea i nga wai o Mokoia, mawhiti atu ki Te Arapueru e karekare mai na ko Te Manukanuka a Hoturoa, kei reira a Taramainuku raua ko Kaiwhare. Ka kawea ki nga.hau o Mangere, e topa ai taku manuhokahoka ki Te Manurewatanga a Tamapahore. Whakaterawhiti taku haere ki Otara-a-nuku, ki Otara-a-rangi koia te tikapatanga o toku tiipuna o Marutuahu. Hoki whenei mai au ki Te Pane o Mataoho, tiro kau ake au ki aku pa whakairo ko Matatukutureia, ko Matukurua nga nohoanga tapu o Huakaiwaka, o Nga Tini-o-toitehuatahi, o Maruiwi kua ngaro katoa ki te reinga.

text: Kia kāpuia te reo o Tāmaki ki te Tonga: Tāmaki Makaurau ki te Tonga Mai i te Papakuratanga o Mataoho tae noa ki tona Ipu. Mai i Pukaki Tapu o Poutiikeka ki Te Puketapapatanga a Hape. Mai i te tahuna o Torea i nga wai o Mokoia, mawhiti atu ki Te Arapueru e karekare mai na ko Te Manukanuka a Hoturoa, kei reira a Taramainuku raua ko Kaiwhare. Ka kawea ki nga.hau o Mangere, e topa ai taku manuhokahoka ki Te Manurewatanga a Tamapahore. Whakaterawhiti taku haere ki Otara-a-nuku, ki Otara-a-rangi koia te tikapatanga o toku tiipuna o Marutuahu. Hoki whenei mai au ki Te Pane o Mataoho, tiro kau ake au ki aku pa whakairo ko Matatukutureia, ko Matukurua nga nohoanga tapu o Huakaiwaka, o Nga Tini-o-toitehuatahi, o Maruiwi kua ngaro katoa ki te reinga.

year: 2017