title: On-farm fractionation of milk components

authors: Chand, Amita

subjects: on-farm

abstract: Methods for on-farm extraction of low-concentration (minor) proteins from raw whole bovine milk directly after milking were explored. These minor proteins have high commercial value. Lactoferrin (LF) and lactoperoxidase (LP) were used as model proteins for extraction using cation exchange chromatography.
Laboratory fractionations showed that milk could be processed by conventional column chromatography without excessive column backpressures if resin with large particles sizes were used and the temperature was high enough so fat in the milk was malleable; ideally the milk should be near the secretion temperature of 37oC. Processing parameters such as equilibrium and dynamic capacities were determined for SP Sepharose ™ (GE Healthcare Technologies) and Bio Rex 70 (BioRad Laboratories) resins. SP Sepharose Big Beads (SP BB) were found to be more suitable than BR 70, for raw whole milk processing due to the larger size (200 um). 
Design considerations showed that column chromatography was not the most practical method for on-farm processing of fresh, raw whole milk. Trials with a single-stage stirred tank showed that SP BB resin could extract up to 65% of LF (initial LF concentration of 0.5 mg/mL) with a 10-minute adsorption time. The composite non-linear (CNL) model of Rowe et al. (1999) was used to describe LF uptake by SP BB resin in raw whole milk with initial LF concentrations of 0 to 1.0 mg/mL and resin:milk volume ratios of 0.010, 0.012, 0.017 and 0.024 over 45-minute contact times. The CNL model could be used to predict LF yields if initial feed concentration, milk and resin volumes, and contact times were known. Laboratory extractions showed that processing did not significantly affect bulk milk composition (fat, protein, lactose and total solids), indicating that the milk could be used for conventional processing after the minor proteins had been extracted.
Resin cleaning and regeneration studies, using a procedure similar to that recommended by the resin supplier, showed that the Sepharose resin had not degraded and there was no significant decrease in binding capacity after 50 extraction cycles.
A Protein Fractionation Robot (PFR) prototype based on a single-stage stirred tank and the operating parameters obtained from the laboratory trials was designed, assembled and coupled to an Automated Milking System (AMS) to process fresh, raw whole milk from individual cows immediately after milking. The LF and LP extracted from the milk from 16 individual cows were 19.7 - 55.2% (35.6   10.2%) and 21.2 - 99.5% (87.1   12.0%) respectively. Generally, higher extraction levels were obtained at higher resin:milk ratios. The amount of LF extracted on-farm agreed within 14.1   9.8% of those predicted by the CNL model, with predicted values generally being higher. The experimental on-farm adsorption values were calculated using data of LF recovered after elution, so differences between actual and predicted values may be due to losses during post-adsorption processing.
Economic feasibility studies, based on experimental data from the PFR and realistic wholesale prices for LF and LP ($400 and $150/kg respectively) showed that PFR-based processing is economically viable if the farmer is paid for the LF and LP produced as well as the bulk milk. This system would have a payback period of approximately five years and an internal rate of return of 14.5%. Further case studies determined the sensitivity of the economics to various operating parameters and value/cost assumptions, including producing recombinant human protein from transgenic bovine milk. These studies showed that the higher the value of the processed raw milk, the higher the absorptive capacity of the resin, and the higher the value of the extracted protein, the more favourable the economics. In the extreme case of producing a very high value therapeutic protein (e.g. $20 000), the payback period could be as low as 0.3 years, with an internal rate of return of 818%.

text: On-farm fractionation of milk components Methods for on-farm extraction of low-concentration (minor) proteins from raw whole bovine milk directly after milking were explored. These minor proteins have high commercial value. Lactoferrin (LF) and lactoperoxidase (LP) were used as model proteins for extraction using cation exchange chromatography.
Laboratory fractionations showed that milk could be processed by conventional column chromatography without excessive column backpressures if resin with large particles sizes were used and the temperature was high enough so fat in the milk was malleable; ideally the milk should be near the secretion temperature of 37oC. Processing parameters such as equilibrium and dynamic capacities were determined for SP Sepharose ™ (GE Healthcare Technologies) and Bio Rex 70 (BioRad Laboratories) resins. SP Sepharose Big Beads (SP BB) were found to be more suitable than BR 70, for raw whole milk processing due to the larger size (200 um). 
Design considerations showed that column chromatography was not the most practical method for on-farm processing of fresh, raw whole milk. Trials with a single-stage stirred tank showed that SP BB resin could extract up to 65% of LF (initial LF concentration of 0.5 mg/mL) with a 10-minute adsorption time. The composite non-linear (CNL) model of Rowe et al. (1999) was used to describe LF uptake by SP BB resin in raw whole milk with initial LF concentrations of 0 to 1.0 mg/mL and resin:milk volume ratios of 0.010, 0.012, 0.017 and 0.024 over 45-minute contact times. The CNL model could be used to predict LF yields if initial feed concentration, milk and resin volumes, and contact times were known. Laboratory extractions showed that processing did not significantly affect bulk milk composition (fat, protein, lactose and total solids), indicating that the milk could be used for conventional processing after the minor proteins had been extracted.
Resin cleaning and regeneration studies, using a procedure similar to that recommended by the resin supplier, showed that the Sepharose resin had not degraded and there was no significant decrease in binding capacity after 50 extraction cycles.
A Protein Fractionation Robot (PFR) prototype based on a single-stage stirred tank and the operating parameters obtained from the laboratory trials was designed, assembled and coupled to an Automated Milking System (AMS) to process fresh, raw whole milk from individual cows immediately after milking. The LF and LP extracted from the milk from 16 individual cows were 19.7 - 55.2% (35.6   10.2%) and 21.2 - 99.5% (87.1   12.0%) respectively. Generally, higher extraction levels were obtained at higher resin:milk ratios. The amount of LF extracted on-farm agreed within 14.1   9.8% of those predicted by the CNL model, with predicted values generally being higher. The experimental on-farm adsorption values were calculated using data of LF recovered after elution, so differences between actual and predicted values may be due to losses during post-adsorption processing.
Economic feasibility studies, based on experimental data from the PFR and realistic wholesale prices for LF and LP ($400 and $150/kg respectively) showed that PFR-based processing is economically viable if the farmer is paid for the LF and LP produced as well as the bulk milk. This system would have a payback period of approximately five years and an internal rate of return of 14.5%. Further case studies determined the sensitivity of the economics to various operating parameters and value/cost assumptions, including producing recombinant human protein from transgenic bovine milk. These studies showed that the higher the value of the processed raw milk, the higher the absorptive capacity of the resin, and the higher the value of the extracted protein, the more favourable the economics. In the extreme case of producing a very high value therapeutic protein (e.g. $20 000), the payback period could be as low as 0.3 years, with an internal rate of return of 818%.

year: 2006

title: Envisioning VR theatre: Virtual reality as an assistive technology in theatre performance

authors: Srinivasan, Saikrishna

subjects: Virtual Reality

abstract: This research project focuses on exploring the potential artistic advancements provided by the inclusion and incorporation of Virtual Reality (VR) within theatre. The incorporation of VR technology into theatre performance ultimately aims to alter a theatre experience by adding spatial presence for audiences and adding new production considerations for theatre practitioners. This project investigated the conceptual nature and practical aspects of using 360-degree cameras placed on stage to enable virtual teleportation onto the stage from the audience or remote viewing.
This thesis constitutes a PhD ‘with publication’ and includes reprints of quality assured peer reviewed scholarly articles produced at different stages on the project. The first study examines the early development and use of three-dimensional theatre prototyping to identify the technical requirements for VR theatre. A digital theatrical prototype was designed, along with mathematical calculations to determine the optimal placement of 360-degree cameras on stage. The study reveals multiple camera angles that can be utilized to capture a 360-degree/VR theatre experience.
Building upon the findings of the first study, the second study focuses on practical and technical factors such as visibility, sound, lighting, and visual intensity to enhance audience access and proximity to live performances. Different camera positions were found to be suitable for different theatre styles. The study suggests that the application of VR technology in theatre stages can yield highly satisfying results, as reported by participating actors.
Following initial prototyping and planning, the PhD then experienced the impact of COVID-19 that prevented planned collaboration with local theatre practitioners that would have enabled a substantive practical application of VR, technical refinements and, user and audience testing. However, in response to the impact of COVID-19 on the theatre industry, the third study investigated the use of virtually designed spaces and the inclusion of 3D avatars/characters or real actors in performances. It explores the creation of photorealistic virtual worlds that mimic real-world environments and examines audience perceptions of virtual theatre experiences. The study presents a modernization of theatre performance by incorporating 3D virtual spaces, akin to VR games, but with limited interactive freedom for the audience.
The fourth and final study involved the development of a post-pandemic experimental theatre performance based on the insights and data collected from the previous studies. It focuses on the virtual teleportation of an audience onto the stage during a performance, facilitated by VR headsets and a 360-degree camera mounted on the actor. Within the experiment participants experienced the performance through VR headsets. The audience and theatre practitioners were then invited to participate in a post interview session, to note their feedback and comments regarding the experience. Despite the limited number of participants due to COVID-19 restrictions, the study highlighted the positive nature of the VR experience pointing to the possibilities for increased virtual access to live theatre beyond this research.
Overall, the results of these studies shed light on key practical considerations involved in the integration of VR into theatre performances. The approaches taken in each study support and guide best practice but also opens up VR-assisted theatre to grass roots level community and local theatre so that VR can become a common consideration and tool for all forms of theatre.

text: Envisioning VR theatre: Virtual reality as an assistive technology in theatre performance This research project focuses on exploring the potential artistic advancements provided by the inclusion and incorporation of Virtual Reality (VR) within theatre. The incorporation of VR technology into theatre performance ultimately aims to alter a theatre experience by adding spatial presence for audiences and adding new production considerations for theatre practitioners. This project investigated the conceptual nature and practical aspects of using 360-degree cameras placed on stage to enable virtual teleportation onto the stage from the audience or remote viewing.
This thesis constitutes a PhD ‘with publication’ and includes reprints of quality assured peer reviewed scholarly articles produced at different stages on the project. The first study examines the early development and use of three-dimensional theatre prototyping to identify the technical requirements for VR theatre. A digital theatrical prototype was designed, along with mathematical calculations to determine the optimal placement of 360-degree cameras on stage. The study reveals multiple camera angles that can be utilized to capture a 360-degree/VR theatre experience.
Building upon the findings of the first study, the second study focuses on practical and technical factors such as visibility, sound, lighting, and visual intensity to enhance audience access and proximity to live performances. Different camera positions were found to be suitable for different theatre styles. The study suggests that the application of VR technology in theatre stages can yield highly satisfying results, as reported by participating actors.
Following initial prototyping and planning, the PhD then experienced the impact of COVID-19 that prevented planned collaboration with local theatre practitioners that would have enabled a substantive practical application of VR, technical refinements and, user and audience testing. However, in response to the impact of COVID-19 on the theatre industry, the third study investigated the use of virtually designed spaces and the inclusion of 3D avatars/characters or real actors in performances. It explores the creation of photorealistic virtual worlds that mimic real-world environments and examines audience perceptions of virtual theatre experiences. The study presents a modernization of theatre performance by incorporating 3D virtual spaces, akin to VR games, but with limited interactive freedom for the audience.
The fourth and final study involved the development of a post-pandemic experimental theatre performance based on the insights and data collected from the previous studies. It focuses on the virtual teleportation of an audience onto the stage during a performance, facilitated by VR headsets and a 360-degree camera mounted on the actor. Within the experiment participants experienced the performance through VR headsets. The audience and theatre practitioners were then invited to participate in a post interview session, to note their feedback and comments regarding the experience. Despite the limited number of participants due to COVID-19 restrictions, the study highlighted the positive nature of the VR experience pointing to the possibilities for increased virtual access to live theatre beyond this research.
Overall, the results of these studies shed light on key practical considerations involved in the integration of VR into theatre performances. The approaches taken in each study support and guide best practice but also opens up VR-assisted theatre to grass roots level community and local theatre so that VR can become a common consideration and tool for all forms of theatre.

year: 2024

title: Lost in translation or lack of nuance? Perception of (near-)synonymy of common te reo Māori loanwords in New Zealand English

authors: Pohl, Alexander

abstract: Ongoing contact between languages and their speakers inevitably leads to a flow of words being borrowed from one language into another. Research in the field of language contact has established and documented the kinds of borrowed words that stick in their recipient language (Haspelmath 2009; Winford 2010). However, one question that has received comparatively less attention is how a newly adopted loanword relates to the structure of the existing lexicon it enters. In recent years, language contact research has thus seen a paradigm shift towards an onomasiological perspective (see Anderson et al. 2017; Crombez et al. 2022; Zenner et al. 2023). Within this approach, most studies looking at lexical variation between loanwords and their (near-)synonyms employ corpus linguistics methods (e.g. Onysko & Winter-Froemel 2011; Soares da Silva 2013). However, there is variation in how these corpus studies have investigated loanword meanings, and, to our knowledge, there has been almost no experimental research focussed specifically on the relationship between loanwords and their (near-)synonyms to date. This project presents a large-scale experimental approach from a case-study examining Māori loanwords borrowed into New Zealand English (NZE). NZE presents an unusual language contact situation because it involves a dominant language (NZE) receiving an exceptionally high amount of lexical material (words and phrases) from an Indigenous language: Māori. Further adding to the uniqueness of this contact situation is the fact that only a fraction of the population (<10%) speaks Māori fluently (Statistics New Zealand 2019). Nonetheless, Māori loanwords are ubiquitous, salient, and probably still increasing within the NZE lexicon (Macalister 2007; Trye et al. 2019). One important dimension of this set of borrowings are their semantic characteristics, which straddle three main classes of words: flora and fauna (mānuka ‘tea tree’), material culture (maunga ‘mountain’) and social culture (mana ‘power’).  Here, we report on a Qualtrics experiment devised to probe the perceived relationship between 60 Māori loanwords and their NZE equivalents, as ascertained from published sources. The synonymy judgements were elicited for pairs (e.g. whānau – family) using an analogue slider (ranging from 0 = non-synonymous to 100 = fully synonymous). Data was collected using a three-tiered questionnaire: 1) assessing synonymy of loanwords; 2) eliciting strategies for semantic assessments; and 3) collating background information about participants. Following cleaning and exclusions, the dataset showed a skew towards female participants, and for this reason, we focus our statistical analysis on the core sample containing (n = 170) young women (18-24 years). The results of the synonymy judgements analysis consist of qualitative inspections of box plots in relation to the semantic characteristics of Māori words in question, as well as sociodemographic variables of interest, specifically: years lived in Aotearoa NZ, and a composite score capturing exposure to and attitude towards Māori (TRMO). Preliminary findings suggest that many people think Māori loanwords are (highly) synonymous with NZE lexical alternatives. Our regression analyses exhibit evidence that years lived in Aotearoa NZ (p = 0.0054) and TRMO (p = 8.26e-10) can predict knowledge of Māori words. No statistically significant evidence was found for any predictors regarding the scores of the synonymy judgements; instead, we found that the random effects account for most of the variation. This is indicative of idiosyncratic loanword behaviour, in line with situations where variation is in-flux. These findings will be complemented by qualitative analyses of comments provided by participants in the open answer section (2). The talk will conclude with a summary of contributions to the general understanding of loanword meanings and contact-induced lexical change.

text: Lost in translation or lack of nuance? Perception of (near-)synonymy of common te reo Māori loanwords in New Zealand English Ongoing contact between languages and their speakers inevitably leads to a flow of words being borrowed from one language into another. Research in the field of language contact has established and documented the kinds of borrowed words that stick in their recipient language (Haspelmath 2009; Winford 2010). However, one question that has received comparatively less attention is how a newly adopted loanword relates to the structure of the existing lexicon it enters. In recent years, language contact research has thus seen a paradigm shift towards an onomasiological perspective (see Anderson et al. 2017; Crombez et al. 2022; Zenner et al. 2023). Within this approach, most studies looking at lexical variation between loanwords and their (near-)synonyms employ corpus linguistics methods (e.g. Onysko & Winter-Froemel 2011; Soares da Silva 2013). However, there is variation in how these corpus studies have investigated loanword meanings, and, to our knowledge, there has been almost no experimental research focussed specifically on the relationship between loanwords and their (near-)synonyms to date. This project presents a large-scale experimental approach from a case-study examining Māori loanwords borrowed into New Zealand English (NZE). NZE presents an unusual language contact situation because it involves a dominant language (NZE) receiving an exceptionally high amount of lexical material (words and phrases) from an Indigenous language: Māori. Further adding to the uniqueness of this contact situation is the fact that only a fraction of the population (<10%) speaks Māori fluently (Statistics New Zealand 2019). Nonetheless, Māori loanwords are ubiquitous, salient, and probably still increasing within the NZE lexicon (Macalister 2007; Trye et al. 2019). One important dimension of this set of borrowings are their semantic characteristics, which straddle three main classes of words: flora and fauna (mānuka ‘tea tree’), material culture (maunga ‘mountain’) and social culture (mana ‘power’).  Here, we report on a Qualtrics experiment devised to probe the perceived relationship between 60 Māori loanwords and their NZE equivalents, as ascertained from published sources. The synonymy judgements were elicited for pairs (e.g. whānau – family) using an analogue slider (ranging from 0 = non-synonymous to 100 = fully synonymous). Data was collected using a three-tiered questionnaire: 1) assessing synonymy of loanwords; 2) eliciting strategies for semantic assessments; and 3) collating background information about participants. Following cleaning and exclusions, the dataset showed a skew towards female participants, and for this reason, we focus our statistical analysis on the core sample containing (n = 170) young women (18-24 years). The results of the synonymy judgements analysis consist of qualitative inspections of box plots in relation to the semantic characteristics of Māori words in question, as well as sociodemographic variables of interest, specifically: years lived in Aotearoa NZ, and a composite score capturing exposure to and attitude towards Māori (TRMO). Preliminary findings suggest that many people think Māori loanwords are (highly) synonymous with NZE lexical alternatives. Our regression analyses exhibit evidence that years lived in Aotearoa NZ (p = 0.0054) and TRMO (p = 8.26e-10) can predict knowledge of Māori words. No statistically significant evidence was found for any predictors regarding the scores of the synonymy judgements; instead, we found that the random effects account for most of the variation. This is indicative of idiosyncratic loanword behaviour, in line with situations where variation is in-flux. These findings will be complemented by qualitative analyses of comments provided by participants in the open answer section (2). The talk will conclude with a summary of contributions to the general understanding of loanword meanings and contact-induced lexical change.

year: 2024