title: Talking 'facts': identity and rationality in industry perspectives on genetic modification

authors: Henderson, Alison

subjects: identity

abstract: Despite the potential political impact of industry attempts to influence public policy about genetic modification, little research has focused on critical understanding of industry perspectives. This article explores the rhetorical and discursive construction of public messages about this controversial issue by two major New Zealand export industries. The kiwifruit industry advocates a very cautious public policy position, while the dairy industry has been a strong advocate for the commercial development of genetic modification. We demonstrate that these industries draw on multiple identities and rationalities to negotiate, express, and elaborate their positions on genetic modification, informed variously by discourses of risk, science, the political economy of the marketplace, and images of the ‘natural’ environment. We suggest that exploring how organizations seek to influence controversial socio-political issues through the management of multiple identities may facilitate greater understanding of viewpoints often represented in the media as two distinct polarities; that is as either ‘for’ or ‘against’ the issues.

text: Talking 'facts': identity and rationality in industry perspectives on genetic modification Despite the potential political impact of industry attempts to influence public policy about genetic modification, little research has focused on critical understanding of industry perspectives. This article explores the rhetorical and discursive construction of public messages about this controversial issue by two major New Zealand export industries. The kiwifruit industry advocates a very cautious public policy position, while the dairy industry has been a strong advocate for the commercial development of genetic modification. We demonstrate that these industries draw on multiple identities and rationalities to negotiate, express, and elaborate their positions on genetic modification, informed variously by discourses of risk, science, the political economy of the marketplace, and images of the ‘natural’ environment. We suggest that exploring how organizations seek to influence controversial socio-political issues through the management of multiple identities may facilitate greater understanding of viewpoints often represented in the media as two distinct polarities; that is as either ‘for’ or ‘against’ the issues.

year: 2007

title: Molecular Characterisation of Sex Differentiation Genes in Yellowtail Kingfish (Seriola lalandi)

authors: Elliott, Nicholas B.

subjects: seriola

abstract: Species diversification is crucial for the successful establishment and growth of a finfish aquaculture industry in New Zealand. The yellowtail kingfish Seriola lalandi is a promising candidate species with a fast growth rate, high market value, and is successfully farmed overseas, along with several other Seriola species. To truly domesticate any farmed species, understanding reproduction is vital. Sexual differentiation is a key part of early reproductive development and is characterised by the migration of primordial germ cells through the body to the site of the presumptive gonad, where they proliferate and differentiate into spermatogonia and oogonia. A number of key genes are expressed during sex differentiation, but currently very little molecular research has been conducted on S. lalandi. 

Using bioinformatics approaches, the available gene databases and the implementation of a transcriptomic library prepared from the gonad, ovary and pituitary tissue of S. lalandi, a number of key sex differentiation genes were identified, which included, Vasa, Amh and Cyp19a1a. Primers were designed to each gene, to enable confirmation of the gene sequences, by PCR and RACE-PCR. Using this approach, no confirmed sequence was obtained for Cyp19a1a, however sequences were obtained for part of the Vasa gene and a large part of the Amh gene, including the 3’end. 

The availability of these sex differentiation gene sequences allowed expression of Vasa, Amh and Cyp19a1a to be investigated. Primers were designed using the confirmed sequences for Vasa and Amh, as well as the unconfirmed sequence for Cyp19a1a. These were used in real-time PCR to examine the expression of each gene during development, within larvae at hatching, 3 days post hatch (dph), 12 dph and 18 dph. Expression of each gene was found to increase by 12 dph, but then decrease at 18dph, however, no meaningful significant difference in expression was seen statistically between any of the time points.

Lastly, a protocol was developed for embedding and sectioning of larvae from a range of different ages. A number of attempts were made to optimise an approach, with fish from 3 dph to 60 dph eventually being paraffin embedded, sectioned and stained with either toluidine blue or hematoxylin and eosin (H&E). A number of regions of interest were described, with one showing good similarity to early gonad formation, detailed in other studies. However, due to technical issues and time constraints, no PGCs or presumptive gonad tissues could be positively identified. Future work will use prepared sections to stain for the sex differentiation genes, to gain a better understanding of their role during development. 

This study has established a platform for further work on S. lalandi by creating a framework of molecular tools for studying sex differentiation genes and their expression in this species. It has also worked towards refining the histological techniques required to successfully analyse structural development in larvae. This will be valuable for future studies with techniques such as in-situ hybridisation, assisting in the development of tools for the improvement of S. lalandi aquaculture, for applications such as monosex culture and surrogate broodstock technology.

text: Molecular Characterisation of Sex Differentiation Genes in Yellowtail Kingfish (Seriola lalandi) Species diversification is crucial for the successful establishment and growth of a finfish aquaculture industry in New Zealand. The yellowtail kingfish Seriola lalandi is a promising candidate species with a fast growth rate, high market value, and is successfully farmed overseas, along with several other Seriola species. To truly domesticate any farmed species, understanding reproduction is vital. Sexual differentiation is a key part of early reproductive development and is characterised by the migration of primordial germ cells through the body to the site of the presumptive gonad, where they proliferate and differentiate into spermatogonia and oogonia. A number of key genes are expressed during sex differentiation, but currently very little molecular research has been conducted on S. lalandi. 

Using bioinformatics approaches, the available gene databases and the implementation of a transcriptomic library prepared from the gonad, ovary and pituitary tissue of S. lalandi, a number of key sex differentiation genes were identified, which included, Vasa, Amh and Cyp19a1a. Primers were designed to each gene, to enable confirmation of the gene sequences, by PCR and RACE-PCR. Using this approach, no confirmed sequence was obtained for Cyp19a1a, however sequences were obtained for part of the Vasa gene and a large part of the Amh gene, including the 3’end. 

The availability of these sex differentiation gene sequences allowed expression of Vasa, Amh and Cyp19a1a to be investigated. Primers were designed using the confirmed sequences for Vasa and Amh, as well as the unconfirmed sequence for Cyp19a1a. These were used in real-time PCR to examine the expression of each gene during development, within larvae at hatching, 3 days post hatch (dph), 12 dph and 18 dph. Expression of each gene was found to increase by 12 dph, but then decrease at 18dph, however, no meaningful significant difference in expression was seen statistically between any of the time points.

Lastly, a protocol was developed for embedding and sectioning of larvae from a range of different ages. A number of attempts were made to optimise an approach, with fish from 3 dph to 60 dph eventually being paraffin embedded, sectioned and stained with either toluidine blue or hematoxylin and eosin (H&E). A number of regions of interest were described, with one showing good similarity to early gonad formation, detailed in other studies. However, due to technical issues and time constraints, no PGCs or presumptive gonad tissues could be positively identified. Future work will use prepared sections to stain for the sex differentiation genes, to gain a better understanding of their role during development. 

This study has established a platform for further work on S. lalandi by creating a framework of molecular tools for studying sex differentiation genes and their expression in this species. It has also worked towards refining the histological techniques required to successfully analyse structural development in larvae. This will be valuable for future studies with techniques such as in-situ hybridisation, assisting in the development of tools for the improvement of S. lalandi aquaculture, for applications such as monosex culture and surrogate broodstock technology.

year: 2015

title: Te ahunga atu ki ngā ōhanga oranga Māori: Towards Māori economies of wellbeing - Ngā whānau i te pokapū o te oranga

authors: Spiller, Chellie

abstract: Nau mai ki tēnei kohinga o ngā whakaaro me ngā tikanga e pā ana ki te oranga ohaoha Māori. Ko tō mātou kaupapa matua ko te whānau; e hiahia ana mātou ki te whakaatu i ngā huarahi ki te ora pai mō te whānau i roto i ngā horopaki ohaoha. Ko te ao Māori tō mātou tūāpapa. E whakakao ana tēnei pukapuka i te huhua o ngā reo me ngā tirohanga nō te ao Māori, me ētahi atu wheako e hāngai ana. Kua kotahi mātou ki raro i te taumarumaru o te kaupapa tūturu kotahi: arā, te oranga pai mō ngā whānau. Ko te tūmanako ka tautoko, ka whakaohooho anō hoki tēnei pukapuka i ngā tāngata kia hōhonu atu tā rātou hono ki tēnei kaupapa whaitake, kia whai māramatanga whaihua hoki rātou.

Welcome to this collection of thoughts and practices related to Māori economic life. Our primary focus is the whānau; we want to show ways to live well for the whānau in economic contexts. Our foundation is te ao Māori. This book brings together many voices and perspectives from the Māori world and other relevant experiences. We are united in one true purpose: the good livelihood of whānau. We hope this book will support and inspire people to connect more deeply with this critical topic and gain valuable insights.

text: Te ahunga atu ki ngā ōhanga oranga Māori: Towards Māori economies of wellbeing - Ngā whānau i te pokapū o te oranga Nau mai ki tēnei kohinga o ngā whakaaro me ngā tikanga e pā ana ki te oranga ohaoha Māori. Ko tō mātou kaupapa matua ko te whānau; e hiahia ana mātou ki te whakaatu i ngā huarahi ki te ora pai mō te whānau i roto i ngā horopaki ohaoha. Ko te ao Māori tō mātou tūāpapa. E whakakao ana tēnei pukapuka i te huhua o ngā reo me ngā tirohanga nō te ao Māori, me ētahi atu wheako e hāngai ana. Kua kotahi mātou ki raro i te taumarumaru o te kaupapa tūturu kotahi: arā, te oranga pai mō ngā whānau. Ko te tūmanako ka tautoko, ka whakaohooho anō hoki tēnei pukapuka i ngā tāngata kia hōhonu atu tā rātou hono ki tēnei kaupapa whaitake, kia whai māramatanga whaihua hoki rātou.

Welcome to this collection of thoughts and practices related to Māori economic life. Our primary focus is the whānau; we want to show ways to live well for the whānau in economic contexts. Our foundation is te ao Māori. This book brings together many voices and perspectives from the Māori world and other relevant experiences. We are united in one true purpose: the good livelihood of whānau. We hope this book will support and inspire people to connect more deeply with this critical topic and gain valuable insights.

year: 2025