title: The effect of complement C5a on mitochondrial functions of PC12 cells

authors: Martinus, Ryan Dennis

subjects: C5a

abstract: C5a is thought to play a role during complement-activated neuronal apoptotic cell death in the central nervous system. The mechanisms responsible are however not well-understood. As mitochondria play a key role during apoptosis, we investigated mitochondria as a potential target for C5a. Using PC12 cells, we demonstrated that exposure to C5a led to inhibition of mitochondrial respiration, dehydrogenase and cytochrome c oxidase activities. Interestingly, an increase in expression of the mitochondrial stress protein chaperonin 60 was also observed, confirming a marked effect of C5a on mitochondrial functions. These observations are the first documented intracellular effects noted for the complement molecule C5a in in-vitro cultured cells.

text: The effect of complement C5a on mitochondrial functions of PC12 cells C5a is thought to play a role during complement-activated neuronal apoptotic cell death in the central nervous system. The mechanisms responsible are however not well-understood. As mitochondria play a key role during apoptosis, we investigated mitochondria as a potential target for C5a. Using PC12 cells, we demonstrated that exposure to C5a led to inhibition of mitochondrial respiration, dehydrogenase and cytochrome c oxidase activities. Interestingly, an increase in expression of the mitochondrial stress protein chaperonin 60 was also observed, confirming a marked effect of C5a on mitochondrial functions. These observations are the first documented intracellular effects noted for the complement molecule C5a in in-vitro cultured cells.

year: 2011-08

title: The impact of background and context on car distance estimation

authors: Zhang, Fan

subjects: Distance Estimation

abstract: It is well established that people underestimate the distance to objects depicted in virtual environments and two-dimensional (2D) displays. The reasons for the underestimation are still not fully understood. It is becoming more common to use virtual environment displays for driver training and testing and so understanding the distortion of perceived space that occurs in these displays is vital. We need to know what aspects of the display cause the observer to misperceive the distance to objects in the simulated environments. The research reported in this thesis investigated how people estimate distance between themselves and a car in front of them, within a number of differing environmental contexts.  Four experiments were run using virtual environment displays of various kinds and a fifth experiment was run in a real-world setting.
It was found that distance underestimation when viewing 2D displays is very common, even when familiar objects such as cars are used as the targets. The experiments also verified that people have a greater underestimation of distance in a virtual environment compared to a real-world setting. A surprising and somewhat counterintuitive result was that people underestimate distance more when the scene depicts forward motion of the observer compared to a static view.  The research also identified a number of visual features in the display (e.g., texture information) and aspects of the display (e.g., field of view) that affected the perception of distance or that had no effect. The findings should help the designers of driver-training simulators and testing equipment to better understand the types of errors that can potentially occur when humans view two-dimensional virtual environment displays.

text: The impact of background and context on car distance estimation It is well established that people underestimate the distance to objects depicted in virtual environments and two-dimensional (2D) displays. The reasons for the underestimation are still not fully understood. It is becoming more common to use virtual environment displays for driver training and testing and so understanding the distortion of perceived space that occurs in these displays is vital. We need to know what aspects of the display cause the observer to misperceive the distance to objects in the simulated environments. The research reported in this thesis investigated how people estimate distance between themselves and a car in front of them, within a number of differing environmental contexts.  Four experiments were run using virtual environment displays of various kinds and a fifth experiment was run in a real-world setting.
It was found that distance underestimation when viewing 2D displays is very common, even when familiar objects such as cars are used as the targets. The experiments also verified that people have a greater underestimation of distance in a virtual environment compared to a real-world setting. A surprising and somewhat counterintuitive result was that people underestimate distance more when the scene depicts forward motion of the observer compared to a static view.  The research also identified a number of visual features in the display (e.g., texture information) and aspects of the display (e.g., field of view) that affected the perception of distance or that had no effect. The findings should help the designers of driver-training simulators and testing equipment to better understand the types of errors that can potentially occur when humans view two-dimensional virtual environment displays.

year: 2014

title: The relevance of discourse analysis to the teaching and learning of te reo Māori: A text-centred example

authors: Greensill, Hine-iti-moana

subjects: Māori language

abstract: In this paper, I analyse a single text written in te reo Māori in terms of two different approaches derived from discourse analysis (discourse macro-patterning and discourse relations and their signalling) in order to demonstrate the importance of deriving a discourse-centred methodology for teaching learners of Māori what is involved in interpreting and creating coherent discourses.

text: The relevance of discourse analysis to the teaching and learning of te reo Māori: A text-centred example In this paper, I analyse a single text written in te reo Māori in terms of two different approaches derived from discourse analysis (discourse macro-patterning and discourse relations and their signalling) in order to demonstrate the importance of deriving a discourse-centred methodology for teaching learners of Māori what is involved in interpreting and creating coherent discourses.

year: 2007