title: Phage display systems for antibody engineering and evaluation

authors: Hanning, Kyrin Rene

subjects: Protein engineering

abstract: Antibodies are versatile effector molecules of the adaptive immune system, distinguished by their capacity to recognise and engage diverse antigens with high specificity and affinity. The widespread adoption of antibodies, and their various formats, in diagnostics and therapeutics has been made possible through the co-operation of experimental and computational molecular engineering techniques. Functional screening of variant antibody libraries has proven essential for successful antibody development. Several protein display technologies have been deployed for this important screening phase, including phage display. Whilst one of the older approaches, phage display still offers many advantages over alternatives and synergises well with other advances in molecular biology techniques. This thesis demonstrates how phage display, when paired with modern techniques in novel ways, remains an extremely flexible and powerful tool for engineering and evaluating antibodies.

text: Phage display systems for antibody engineering and evaluation Antibodies are versatile effector molecules of the adaptive immune system, distinguished by their capacity to recognise and engage diverse antigens with high specificity and affinity. The widespread adoption of antibodies, and their various formats, in diagnostics and therapeutics has been made possible through the co-operation of experimental and computational molecular engineering techniques. Functional screening of variant antibody libraries has proven essential for successful antibody development. Several protein display technologies have been deployed for this important screening phase, including phage display. Whilst one of the older approaches, phage display still offers many advantages over alternatives and synergises well with other advances in molecular biology techniques. This thesis demonstrates how phage display, when paired with modern techniques in novel ways, remains an extremely flexible and powerful tool for engineering and evaluating antibodies.

year: 2025

title: Where else have TDRs been applied

authors: Martelli, Phillip

abstract: Phillip originally hails from Reporoa. His upbringing and living rurally in the Western Bay of Plenty District for many years, including developing his own orchard, gives him a strong appreciation of the rural environment. Phillip has been with the Western Bay of Plenty District Council for 23 years. As the policy planning manager he has been involved with the evolution of the Protection Lot Rule in district plans from the very beginning. Originally they were bush protection lots for on-site subdivision but now they are a tradable commodity allowing the rights to a title to be transferred to elsewhere in the district.

text: Where else have TDRs been applied Phillip originally hails from Reporoa. His upbringing and living rurally in the Western Bay of Plenty District for many years, including developing his own orchard, gives him a strong appreciation of the rural environment. Phillip has been with the Western Bay of Plenty District Council for 23 years. As the policy planning manager he has been involved with the evolution of the Protection Lot Rule in district plans from the very beginning. Originally they were bush protection lots for on-site subdivision but now they are a tradable commodity allowing the rights to a title to be transferred to elsewhere in the district.

year: 2013

title: A study of iwi communication between Te Tau Ihu iwi and intergenerational diasporic whānau

authors: Wright, Karamea Moana

subjects: diaspora

abstract: This study looks at the online communication methods used between Te Tau Ihu iwi and intergenerational uri who live in the diaspora. Preferences and perceptions of methods of communication utilized by both iwi, and tāngata who live in the overseas diaspora were explored. Most important are the implications for uri living away from Aotearoa, and their desire to remain connected to iwi in New Zealand. This is a mixed methods study, comprising of a survey of fifty-eight emigrant respondents living abroad, and interviews with one member of the first, second, and third generations of emigrants to the United States, with affiliation to at least one of the eight tribes who claim tāngata whenua status of Te Tau Ihu.

The implications of the findings from the study provide both iwi and tāngata the opportunity to create a synergistic relationship of communicating. Assisting iwi to develop and implement a strategy for relevant communication, and iwi members engaging in a dialogue with iwi about relevance of communication is critical in strengthening ties between iwi and future generations regardless of location. “He aha te mea nui, he tangata, he tangata.”

text: A study of iwi communication between Te Tau Ihu iwi and intergenerational diasporic whānau This study looks at the online communication methods used between Te Tau Ihu iwi and intergenerational uri who live in the diaspora. Preferences and perceptions of methods of communication utilized by both iwi, and tāngata who live in the overseas diaspora were explored. Most important are the implications for uri living away from Aotearoa, and their desire to remain connected to iwi in New Zealand. This is a mixed methods study, comprising of a survey of fifty-eight emigrant respondents living abroad, and interviews with one member of the first, second, and third generations of emigrants to the United States, with affiliation to at least one of the eight tribes who claim tāngata whenua status of Te Tau Ihu.

The implications of the findings from the study provide both iwi and tāngata the opportunity to create a synergistic relationship of communicating. Assisting iwi to develop and implement a strategy for relevant communication, and iwi members engaging in a dialogue with iwi about relevance of communication is critical in strengthening ties between iwi and future generations regardless of location. “He aha te mea nui, he tangata, he tangata.”

year: 2019