title: Developing usability studies via formal models of UIs

authors: Bowen, Judy

subjects: computer science

abstract: Developing usability studies to evaluate software is a task requiring a wide variety of skills. For software developers who are not used to taking a user-centred approach to development it is often easier and more convenient to dismiss the use of user evaluation as too time-consuming or too hard. This is even more likely to be the case for developers who take a formal approach to software development, which is generally not focused on interface or usability concerns. In this paper we present an early investigation into the use of formal models of user interface designs as the basis for designing software evaluation studies. We have undertaken a comparison study to find out whether a useful study can be derived in this way and whether or not further investigation into this is worthwhile, and we present the results here.

text: Developing usability studies via formal models of UIs Developing usability studies to evaluate software is a task requiring a wide variety of skills. For software developers who are not used to taking a user-centred approach to development it is often easier and more convenient to dismiss the use of user evaluation as too time-consuming or too hard. This is even more likely to be the case for developers who take a formal approach to software development, which is generally not focused on interface or usability concerns. In this paper we present an early investigation into the use of formal models of user interface designs as the basis for designing software evaluation studies. We have undertaken a comparison study to find out whether a useful study can be derived in this way and whether or not further investigation into this is worthwhile, and we present the results here.

year: 2010

title: Development of a qPCR method to measure mitochondrial and genomic DNA damage with application to chemotherapy-induced DNA damage and cryopreserved cells

authors: Evans, Stephen Owen

subjects: LORD-Q

abstract: DNA damage quantitation assays such as the comet assay have focused on the measurement of total nuclear damage per cell. The adoption of PCR-based techniques to quantify DNA damage has enabled sequence- and organelle-specific assessment of DNA lesions. Here we report on an adaptation of a qPCR technique to assess DNA damage in nuclear and mitochondrial targets relative to control. Novel aspects of this assay include application of the assay to the Rotor-Gene platform with optimized DNA polymerase/fluorophore/primer set combination in a touchdown PCR protocol. Assay validation was performed using ultraviolet C radiation in A549 and THP1 cancer cell lines. A comparison was made to the comet assay applied to peripheral blood mononuclear cells, and an estimation of the effects of cryopreservation on ultraviolet C-induced DNA damage was carried out. Finally, dose responses for DNA damage were measured in peripheral blood mononuclear cells following exposure to the cytotoxic agents bleomycin and cisplatin. We show reproducible experimental outputs across the tested conditions and concordance with published findings with respect to mitochondrial and nuclear genotoxic susceptibilities. The application of this DNA damage assay to a wide range of clinical and laboratory-derived samples is both feasible and resource-efficient.

text: Development of a qPCR method to measure mitochondrial and genomic DNA damage with application to chemotherapy-induced DNA damage and cryopreserved cells DNA damage quantitation assays such as the comet assay have focused on the measurement of total nuclear damage per cell. The adoption of PCR-based techniques to quantify DNA damage has enabled sequence- and organelle-specific assessment of DNA lesions. Here we report on an adaptation of a qPCR technique to assess DNA damage in nuclear and mitochondrial targets relative to control. Novel aspects of this assay include application of the assay to the Rotor-Gene platform with optimized DNA polymerase/fluorophore/primer set combination in a touchdown PCR protocol. Assay validation was performed using ultraviolet C radiation in A549 and THP1 cancer cell lines. A comparison was made to the comet assay applied to peripheral blood mononuclear cells, and an estimation of the effects of cryopreservation on ultraviolet C-induced DNA damage was carried out. Finally, dose responses for DNA damage were measured in peripheral blood mononuclear cells following exposure to the cytotoxic agents bleomycin and cisplatin. We show reproducible experimental outputs across the tested conditions and concordance with published findings with respect to mitochondrial and nuclear genotoxic susceptibilities. The application of this DNA damage assay to a wide range of clinical and laboratory-derived samples is both feasible and resource-efficient.

year: 2016

title: How Maui captured the sun: using a MUD for educational simulation

authors: Cunningham, Sally Jo

subjects: computer science

abstract: MUDs (Multi-User Dungeons) are text-based, multi-user communication and modelling programs. This paper investigates the potential of a popular extensible MUD, the LambdaMOO system, as a tool for second language training and for educational simulation gaming.

text: How Maui captured the sun: using a MUD for educational simulation MUDs (Multi-User Dungeons) are text-based, multi-user communication and modelling programs. This paper investigates the potential of a popular extensible MUD, the LambdaMOO system, as a tool for second language training and for educational simulation gaming.

year: 1995-12