title: eDNA-based detection of New Zealand freshwater mussel (kākahi) populations using digital PCR

authors: Ferris, Kelsey

subjects: eDNA

abstract: Globally, freshwater mussels are one of the most endangered taxa due to pollution, nutrient loading, habitat fragmentation, and species introduction attributable to anthropogenic activities. New Zealand freshwater mussels (kākahi) are ecological engineers and integral species in freshwater ecosystems. However, kākahi populations are expected to decline 70% by 2024 in part due to their unique reproductive cycle involving an obligatory phoretic relationship with declining native fish. Kākahi distribution and abundance throughout New Zealand is poorly understood due to time-consuming surveying techniques of concealed and morphologically cryptic species. A rapid and reliable method is required for the detection and identification of these ‘Threatened’ kākahi populations for conservation and management. Therefore, this study aimed to i) validate an environmental DNA (eDNA) based detection method for New Zealand freshwater mussels using digital PCR (dPCR); ii) determine the most appropriate sampling season for kākahi eDNA; and iii) determine whether eDNA could be used to detect effects of fish barriers on kākahi distribution.
In vivo trials determined that filtration of field samples within 24 hours of collection minimised eDNA degradation and that prefiltration effectively removed inhibitory detritus and glochidia. The lowest concentration of eDNA which could accurately be detected to infer abundance (≥3.2 copies/µL) or presence (0.32-3.19 copies/µL) of kākahi eDNA was determined.  Duplicate field samples were collected from the Waingaro River and Kahuhuru Stream at sites downstream of fish barriers and upstream of low-flow or all-flow barriers in December, May, and August to investigate spatial and temporal variation in eDNA concentrations. As hypothesised, December was the most effective month to sample, yielding the highest eDNA concentrations due to reduced dilution and heightened water temperatures. Based on eDNA, kākahi species distribution did not appear correlated with fish barriers, but all-flow barriers likely impeded fish host movement to significantly reduce the total kākahi eDNA concentrations found upstream. 
eDNA detection can be used by regional councils to effectively infer presence/absence of kākahi populations and direct further conventional surveying efforts to the most impactful locations. This method allows rapid determination of distribution and densities at a national scale to aid conservation management of this taonga (treasured) species.

text: eDNA-based detection of New Zealand freshwater mussel (kākahi) populations using digital PCR Globally, freshwater mussels are one of the most endangered taxa due to pollution, nutrient loading, habitat fragmentation, and species introduction attributable to anthropogenic activities. New Zealand freshwater mussels (kākahi) are ecological engineers and integral species in freshwater ecosystems. However, kākahi populations are expected to decline 70% by 2024 in part due to their unique reproductive cycle involving an obligatory phoretic relationship with declining native fish. Kākahi distribution and abundance throughout New Zealand is poorly understood due to time-consuming surveying techniques of concealed and morphologically cryptic species. A rapid and reliable method is required for the detection and identification of these ‘Threatened’ kākahi populations for conservation and management. Therefore, this study aimed to i) validate an environmental DNA (eDNA) based detection method for New Zealand freshwater mussels using digital PCR (dPCR); ii) determine the most appropriate sampling season for kākahi eDNA; and iii) determine whether eDNA could be used to detect effects of fish barriers on kākahi distribution.
In vivo trials determined that filtration of field samples within 24 hours of collection minimised eDNA degradation and that prefiltration effectively removed inhibitory detritus and glochidia. The lowest concentration of eDNA which could accurately be detected to infer abundance (≥3.2 copies/µL) or presence (0.32-3.19 copies/µL) of kākahi eDNA was determined.  Duplicate field samples were collected from the Waingaro River and Kahuhuru Stream at sites downstream of fish barriers and upstream of low-flow or all-flow barriers in December, May, and August to investigate spatial and temporal variation in eDNA concentrations. As hypothesised, December was the most effective month to sample, yielding the highest eDNA concentrations due to reduced dilution and heightened water temperatures. Based on eDNA, kākahi species distribution did not appear correlated with fish barriers, but all-flow barriers likely impeded fish host movement to significantly reduce the total kākahi eDNA concentrations found upstream. 
eDNA detection can be used by regional councils to effectively infer presence/absence of kākahi populations and direct further conventional surveying efforts to the most impactful locations. This method allows rapid determination of distribution and densities at a national scale to aid conservation management of this taonga (treasured) species.

year: 2020

title: Teaching Māori in English-medium schools in New Zealand: Teacher responses to aspects of the curriculum guidelines for te reo Māori in English-medium school settings

authors: Takurua, Nātana

subjects: New Zealand

abstract: Opinions are divided about how the content of language courses should be specified and whether educational authorities should include specifications/ suggestions about language content in national curriculum documents. Issues associated with language content are, however, not ones that can be avoided, particularly as teachers are expected to prepare students for national examinations at the upper levels of schooling and these examinations are necessarily predicated on general expectations about proficiency achievements and more specific expectations about the types of language with which students will be familiar. In this article, we explore the complex nature of some of the issues involved and the ways in which the New Zealand Ministry of Education has sought/ is seeking to resolve them in the case of the curriculum for Māori in English-medium schools. We also report on the responses of a sample of teachers of Māori in secondary schools in New Zealand to the omission of language examples from the final version of the curriculum guidelines document.

text: Teaching Māori in English-medium schools in New Zealand: Teacher responses to aspects of the curriculum guidelines for te reo Māori in English-medium school settings Opinions are divided about how the content of language courses should be specified and whether educational authorities should include specifications/ suggestions about language content in national curriculum documents. Issues associated with language content are, however, not ones that can be avoided, particularly as teachers are expected to prepare students for national examinations at the upper levels of schooling and these examinations are necessarily predicated on general expectations about proficiency achievements and more specific expectations about the types of language with which students will be familiar. In this article, we explore the complex nature of some of the issues involved and the ways in which the New Zealand Ministry of Education has sought/ is seeking to resolve them in the case of the curriculum for Māori in English-medium schools. We also report on the responses of a sample of teachers of Māori in secondary schools in New Zealand to the omission of language examples from the final version of the curriculum guidelines document.

year: 2009-09

title: Heritage/cultural attraction atmospherics: Creating the right environment for the heritage/cultural visitor

authors: Bonn, Mark A.

subjects: heritage/cultural tourism

abstract: The popular press is replete with articles discussing the various remodeling efforts afoot in many of the prominent heritage attractions worldwide. Undoubtedly, museum curators have acknowledged the tremendous role external surroundings can play in the overall experience of the heritage visitor. This study investigates the effect of some basic environmental elements on visitors to heritage sites, illustrating that the heritage attraction’s physical environment plays an important role in determining both visitors’ attitude toward the heritage attraction and future repatronage intentions, as well as their willingness to recommend the experience to friends and relatives. The study findings indicate that environmental cues can be used as a differential tool to perpetuate brand meaning and uniqueness in the minds of the consumer, thereby creating a competitive advantage for the heritage attraction.

text: Heritage/cultural attraction atmospherics: Creating the right environment for the heritage/cultural visitor The popular press is replete with articles discussing the various remodeling efforts afoot in many of the prominent heritage attractions worldwide. Undoubtedly, museum curators have acknowledged the tremendous role external surroundings can play in the overall experience of the heritage visitor. This study investigates the effect of some basic environmental elements on visitors to heritage sites, illustrating that the heritage attraction’s physical environment plays an important role in determining both visitors’ attitude toward the heritage attraction and future repatronage intentions, as well as their willingness to recommend the experience to friends and relatives. The study findings indicate that environmental cues can be used as a differential tool to perpetuate brand meaning and uniqueness in the minds of the consumer, thereby creating a competitive advantage for the heritage attraction.

year: 2007