title: Peptide synthesis with a proteinase from the extremely thermophilic organism Thermus Rt41A

authors: Wilson, Shelley-Ann

subjects: peptide synthesis

abstract: A proteinase isolated from Thermus RT41a was immobilized to controlled pore glass beads and was used in the free and immobilized forms for peptide synthesis. The observed maximum yield was the same in both cases. a number of dipeptides were produced from amino acid esters and amides. The best acyl components, from those tested, were found to be Ac-Phe-OEt and Bz-Ala-OMe. Tur-NH2, Trp-NH2, Leu-pNA, and Val-pNA were all reactive nucleophiles.The kinetically controlled synthesis of Bz-ala-Tyr-NH2 was optimized by studying the effect of pH, temperature, solvent concentration, ionic strength, and nucleophile and acyl donor concentration, ionic strength, and nucleophile and acyl donor concentration on the maximum yield. The initial conditions used were 25 mM Bz–ala-OMe, 25 mM Tyr-NH2, 70°C, pH 8.0, and 10% v/v dimethylformamide (DMF). The optimum conditions were 90% v/v DMF using 80 mM bz-Ala-OMe and 615 mM Tyr-NH2 at 40°C and pH 10. These conditions increased the maximum conversion from 0.75% to 26% (of the original ester concentration). In a number of other cosolvents, the best peptide yields were observed with acetonitrile and ethyl acetate. In 90% acetonitrile similar yields were observed to those in 90% DMF under optimized conditions except that the acyl donor and nucleophile concentrations could be reduced to 25 mM and 100mM, respectively. The effect of the blocking group on the nucleophile was also investigated; -βNA and -pNA as blocking groups improved the yields markedly. The blocking and leaving groups of the acyldonor had no effect on the dipeptide yield.

text: Peptide synthesis with a proteinase from the extremely thermophilic organism Thermus Rt41A A proteinase isolated from Thermus RT41a was immobilized to controlled pore glass beads and was used in the free and immobilized forms for peptide synthesis. The observed maximum yield was the same in both cases. a number of dipeptides were produced from amino acid esters and amides. The best acyl components, from those tested, were found to be Ac-Phe-OEt and Bz-Ala-OMe. Tur-NH2, Trp-NH2, Leu-pNA, and Val-pNA were all reactive nucleophiles.The kinetically controlled synthesis of Bz-ala-Tyr-NH2 was optimized by studying the effect of pH, temperature, solvent concentration, ionic strength, and nucleophile and acyl donor concentration, ionic strength, and nucleophile and acyl donor concentration on the maximum yield. The initial conditions used were 25 mM Bz–ala-OMe, 25 mM Tyr-NH2, 70°C, pH 8.0, and 10% v/v dimethylformamide (DMF). The optimum conditions were 90% v/v DMF using 80 mM bz-Ala-OMe and 615 mM Tyr-NH2 at 40°C and pH 10. These conditions increased the maximum conversion from 0.75% to 26% (of the original ester concentration). In a number of other cosolvents, the best peptide yields were observed with acetonitrile and ethyl acetate. In 90% acetonitrile similar yields were observed to those in 90% DMF under optimized conditions except that the acyl donor and nucleophile concentrations could be reduced to 25 mM and 100mM, respectively. The effect of the blocking group on the nucleophile was also investigated; -βNA and -pNA as blocking groups improved the yields markedly. The blocking and leaving groups of the acyldonor had no effect on the dipeptide yield.

year: 1994

title: Where there's smoke there's fire? Women's experiences of smoking and cessation during pregnancy

authors: Drewer, Amanda

subjects: smoking

abstract: Women who smoke during pregnancy have been identified by the New Zealand Ministry of Health as a priority group for cessation services. In addition to adverse pregnancy outcomes, the effects of cigarette exposure in utero are believed to have health implications throughout life for the developing foetus. The aim of this study was to investigate the factors that affect smoking and cessation during pregnancy.  Fifteen women who had quit, tried to quit, or continued to smoke through a recent pregnancy were interviewed using face-to-face semi-structured interviews. During analysis, several key findings became apparent. Smoking played a central role in the women’s lives both in and out of pregnancy. Women were fearful and concerned about the possible effects on their growing babies. Their concerns contributed to their feelings of guilt and other negative emotions, as did frustrations over not being able to quit, adverse pregnancy outcomes and their perceived failure to meet with social expectations. However, they also doubted their concerns as most had seen, or had had their own experiences of healthy babies being born to mothers who smoked. Nonetheless, their worries led to a range of behavioural changes including spontaneously quitting, adjusting their smoking, hiding their pregnancy to continue smoking, or hiding their smoking. Changing their smoking behaviours enabled women to avoid being judged which was raised in most interviews. In addition, the importance of the context in which women lived and socialised was highlighted, as were the women’s midwives. Healthcare professionals were often spoken about in relation to the mismatch between what women wanted regarding cessation and what they were supplied. A key finding was the range of smoking statuses women presented with. This contrasts with much of the other literature that usually defines women as smokers or quitters. The use of the label ‘smoker’ in society is a generalised term that obscures differences that are important for healthcare practitioners to recognise. As a result of this study, a tool that assesses a woman’s awareness of the risks from smoking in pregnancy and openness to talking about smoking cessation has been developed. The aim is to give maternity care providers a way to broach this sensitive subject in a way that is appropriate for their client.

text: Where there's smoke there's fire? Women's experiences of smoking and cessation during pregnancy Women who smoke during pregnancy have been identified by the New Zealand Ministry of Health as a priority group for cessation services. In addition to adverse pregnancy outcomes, the effects of cigarette exposure in utero are believed to have health implications throughout life for the developing foetus. The aim of this study was to investigate the factors that affect smoking and cessation during pregnancy.  Fifteen women who had quit, tried to quit, or continued to smoke through a recent pregnancy were interviewed using face-to-face semi-structured interviews. During analysis, several key findings became apparent. Smoking played a central role in the women’s lives both in and out of pregnancy. Women were fearful and concerned about the possible effects on their growing babies. Their concerns contributed to their feelings of guilt and other negative emotions, as did frustrations over not being able to quit, adverse pregnancy outcomes and their perceived failure to meet with social expectations. However, they also doubted their concerns as most had seen, or had had their own experiences of healthy babies being born to mothers who smoked. Nonetheless, their worries led to a range of behavioural changes including spontaneously quitting, adjusting their smoking, hiding their pregnancy to continue smoking, or hiding their smoking. Changing their smoking behaviours enabled women to avoid being judged which was raised in most interviews. In addition, the importance of the context in which women lived and socialised was highlighted, as were the women’s midwives. Healthcare professionals were often spoken about in relation to the mismatch between what women wanted regarding cessation and what they were supplied. A key finding was the range of smoking statuses women presented with. This contrasts with much of the other literature that usually defines women as smokers or quitters. The use of the label ‘smoker’ in society is a generalised term that obscures differences that are important for healthcare practitioners to recognise. As a result of this study, a tool that assesses a woman’s awareness of the risks from smoking in pregnancy and openness to talking about smoking cessation has been developed. The aim is to give maternity care providers a way to broach this sensitive subject in a way that is appropriate for their client.

year: 2014

title: Bicultural perspectives on Māori legal research

authors: Mackinnon, Jacquelin

subjects: Māori

abstract: Increasingly, legal research projects undertaken at law school or in practice will involve Māori custom law and/or tikanga Māori.' The role of both Māori custom law and tikanga Māori is most evident in the work of the Māori Land Court in the interpretation and application of legislation relating to Māori land. Increasingly, general statutes incorporate Māori principles and values, such as those to be found in the Resource Management Act 1991, or make explicit reference to the principles of the Treaty of Waitangi. The statutory interpretation function of the Courts in relation to these, and less obvious examples, requires knowledge of tikanga Māori and/or Māori custom law. For any analysis of the work of the Courts or of the legislature, knowledge of tikanga Māori and/or custom law is required. Both Māori custom law and tikanga Māori are preserved by and accessed through the oral tradition. In addition to its role in the Courts and in relation to legislation, the most significant role played by the oral tradition is in the work of the Waitangi Tribunal. The oral tradition also plays an increasingly important part in other areas requiring research. What follows is an introduction to the oral tradition and its role in the legal system of Aotearoa/New Zealand. An understanding of the oral tradition is essential to the construction of a research path that is both ethical and effective.

text: Bicultural perspectives on Māori legal research Increasingly, legal research projects undertaken at law school or in practice will involve Māori custom law and/or tikanga Māori.' The role of both Māori custom law and tikanga Māori is most evident in the work of the Māori Land Court in the interpretation and application of legislation relating to Māori land. Increasingly, general statutes incorporate Māori principles and values, such as those to be found in the Resource Management Act 1991, or make explicit reference to the principles of the Treaty of Waitangi. The statutory interpretation function of the Courts in relation to these, and less obvious examples, requires knowledge of tikanga Māori and/or Māori custom law. For any analysis of the work of the Courts or of the legislature, knowledge of tikanga Māori and/or custom law is required. Both Māori custom law and tikanga Māori are preserved by and accessed through the oral tradition. In addition to its role in the Courts and in relation to legislation, the most significant role played by the oral tradition is in the work of the Waitangi Tribunal. The oral tradition also plays an increasingly important part in other areas requiring research. What follows is an introduction to the oral tradition and its role in the legal system of Aotearoa/New Zealand. An understanding of the oral tradition is essential to the construction of a research path that is both ethical and effective.

year: 2007