title: How is the circular economy emerging in Aotearoa New Zealand? Research Brief

authors: Diprose, G

abstract: The results of the environmental scan show that there is grass-roots activity and that we have some high level conceptualisations of circular economies (CE) however there is little in-between. As such, it is in the middle that activity needs to occur to transition towards a circular economy. We see a need to move beyond business-as-usual with an entity focus to an ‘ecosystem phase.’ This means that for CE, partnerships are needed across business, organisations, Treaty partners, institutions, NGOs and stakeholders to form collaborations around material and people flows in order for ecosystems to form and create circular economies.

text: How is the circular economy emerging in Aotearoa New Zealand? Research Brief The results of the environmental scan show that there is grass-roots activity and that we have some high level conceptualisations of circular economies (CE) however there is little in-between. As such, it is in the middle that activity needs to occur to transition towards a circular economy. We see a need to move beyond business-as-usual with an entity focus to an ‘ecosystem phase.’ This means that for CE, partnerships are needed across business, organisations, Treaty partners, institutions, NGOs and stakeholders to form collaborations around material and people flows in order for ecosystems to form and create circular economies.

year: 2022

title: What does it take to make us listen? The experiences of those leading whole school reform to improve outcomes for indigenous minority students

authors: Buckley, Louise Jane

subjects: Education

abstract: This thesis examines how the Kia Eke Panuku: Building on Success school reform initiative shaped the experiences of groups of educators within mainstream New Zealand secondary schools. These experiences are analysed against a setting of colonisation and historic educational policies of assimilation and integration that have created a situation of education debt. The lack of power for Māori in education is evident. This study highlights the role of the Strategic Change Leadership Team in deconstructing power hierarchies within schools so that educational outcomes can be improved for Māori students. It presents the Strategic Change Leadership Team as the vehicle for conscientisation, resistance and transformative praxis within schools. It shows that in schools where power hierarchies were deconstructed, Māori students were able to enjoy educational success as Māori.

text: What does it take to make us listen? The experiences of those leading whole school reform to improve outcomes for indigenous minority students This thesis examines how the Kia Eke Panuku: Building on Success school reform initiative shaped the experiences of groups of educators within mainstream New Zealand secondary schools. These experiences are analysed against a setting of colonisation and historic educational policies of assimilation and integration that have created a situation of education debt. The lack of power for Māori in education is evident. This study highlights the role of the Strategic Change Leadership Team in deconstructing power hierarchies within schools so that educational outcomes can be improved for Māori students. It presents the Strategic Change Leadership Team as the vehicle for conscientisation, resistance and transformative praxis within schools. It shows that in schools where power hierarchies were deconstructed, Māori students were able to enjoy educational success as Māori.

year: 2020

title: The Structure of the Oligomerization Domain of Lsr2 from Mycobacterium tuberculosis Reveals a Mechanism for Chromosome Organization and Protection

authors: Summers, Emma Louise

subjects: Science & Technology

abstract: Lsr2 is a small DNA-binding protein present in mycobacteria and related actinobacteria that regulates gene expression and influences the organization of bacterial chromatin. Lsr2 is a dimer that binds to AT-rich regions of chromosomal DNA and physically protects DNA from damage by reactive oxygen intermediates (ROI). A recent structure of the C-terminal DNAbinding domain of Lsr2 provides a rationale for its interaction with the minor groove of DNA, its preference for AT-rich tracts, and its similarity to other bacterial nucleoid-associated DNA-binding domains. In contrast, the details of Lsr2 dimerization (and oligomerization) via its N-terminal domain, and the mechanism of Lsr2-mediated chromosomal crosslinking and protection is unknown. We have solved the structure of the N-terminal domain of Lsr2 (N-Lsr2) at 1.73 A˚ resolution using crystallographic ab initio approaches. The structure shows an intimate dimer of two ß–ß–a motifs with no close homologues in the structural databases. The organization of individual N-Lsr2 dimers in the crystal also reveals a mechanism for oligomerization. Proteolytic removal of three N-terminal residues from Lsr2 results in the formation of an anti-parallel b-sheet between neighboring molecules and the formation of linear chains of N-Lsr2. Oligomerization can be artificially induced using low concentrations of trypsin and the arrangement of N-Lsr2 into long chains is observed in both monoclinic and hexagonal crystallographic space groups. In solution, oligomerization of N-Lsr2 is also observed following treatment with trypsin. A change in chromosomal topology after the addition of trypsin to full-length Lsr2-DNA complexes and protection of DNA towards DNAse digestion can be observed using electron microscopy and electrophoresis. These results suggest a mechanism for oligomerization of Lsr2 via protease-activation leading to chromosome compaction and protection, and concomitant down-regulation of large numbers of genes. This mechanism is likely to be relevant under conditions of stress where cellular proteases are known to be upregulated.

text: The Structure of the Oligomerization Domain of Lsr2 from Mycobacterium tuberculosis Reveals a Mechanism for Chromosome Organization and Protection Lsr2 is a small DNA-binding protein present in mycobacteria and related actinobacteria that regulates gene expression and influences the organization of bacterial chromatin. Lsr2 is a dimer that binds to AT-rich regions of chromosomal DNA and physically protects DNA from damage by reactive oxygen intermediates (ROI). A recent structure of the C-terminal DNAbinding domain of Lsr2 provides a rationale for its interaction with the minor groove of DNA, its preference for AT-rich tracts, and its similarity to other bacterial nucleoid-associated DNA-binding domains. In contrast, the details of Lsr2 dimerization (and oligomerization) via its N-terminal domain, and the mechanism of Lsr2-mediated chromosomal crosslinking and protection is unknown. We have solved the structure of the N-terminal domain of Lsr2 (N-Lsr2) at 1.73 A˚ resolution using crystallographic ab initio approaches. The structure shows an intimate dimer of two ß–ß–a motifs with no close homologues in the structural databases. The organization of individual N-Lsr2 dimers in the crystal also reveals a mechanism for oligomerization. Proteolytic removal of three N-terminal residues from Lsr2 results in the formation of an anti-parallel b-sheet between neighboring molecules and the formation of linear chains of N-Lsr2. Oligomerization can be artificially induced using low concentrations of trypsin and the arrangement of N-Lsr2 into long chains is observed in both monoclinic and hexagonal crystallographic space groups. In solution, oligomerization of N-Lsr2 is also observed following treatment with trypsin. A change in chromosomal topology after the addition of trypsin to full-length Lsr2-DNA complexes and protection of DNA towards DNAse digestion can be observed using electron microscopy and electrophoresis. These results suggest a mechanism for oligomerization of Lsr2 via protease-activation leading to chromosome compaction and protection, and concomitant down-regulation of large numbers of genes. This mechanism is likely to be relevant under conditions of stress where cellular proteases are known to be upregulated.

year: 2012-06-13