title: The advent of the Anthropocene in Australasia.

authors: Bostock, Helen C.

abstract: As early as the late 19th Century, several scientists had suggested that humans were starting to influence the physical environment of planet Earth (e.g. Marsh, 1864; Stoppani, 1873; Arrhenius, 1896; Chamberlain, 1897). This idea was resurrected and expanded in 2000 by Paul Crutzen, a Nobel Prize-winning chemist, and the late Eugene Stoermer, a professor of biology specialising in diatoms, who suggested that we had left the Holocene and entered the “Anthropocene” (Crutzen and Stoermer, 2000). As summarised by Steffen et al. (2011) and Wolfe et al. (2013), these iconoclastic scientists were referring to the Anthropocene as the interval of demonstrable human alteration of global biogeochemical cycles, beginning subtly in the late 18th Century following James Watt’s invention of the coal-fired steam engine, and accelerating markedly in the mid-20th Century (termed “The Great Acceleration”). Thus Crutzen and Stoermer (2000) argued that the Anthropocene should be an epoch, and for a starting date at the beginning of the Industrial Revolution (Monastersky, 2015).

text: The advent of the Anthropocene in Australasia. As early as the late 19th Century, several scientists had suggested that humans were starting to influence the physical environment of planet Earth (e.g. Marsh, 1864; Stoppani, 1873; Arrhenius, 1896; Chamberlain, 1897). This idea was resurrected and expanded in 2000 by Paul Crutzen, a Nobel Prize-winning chemist, and the late Eugene Stoermer, a professor of biology specialising in diatoms, who suggested that we had left the Holocene and entered the “Anthropocene” (Crutzen and Stoermer, 2000). As summarised by Steffen et al. (2011) and Wolfe et al. (2013), these iconoclastic scientists were referring to the Anthropocene as the interval of demonstrable human alteration of global biogeochemical cycles, beginning subtly in the late 18th Century following James Watt’s invention of the coal-fired steam engine, and accelerating markedly in the mid-20th Century (termed “The Great Acceleration”). Thus Crutzen and Stoermer (2000) argued that the Anthropocene should be an epoch, and for a starting date at the beginning of the Industrial Revolution (Monastersky, 2015).

year: 2015-07-21

title: General Anaesthetic Modulation of Memory-Related Gene Expression Using an In vitro Brain Slice Model

authors: Stayte, Liam Thomas Oliver

subjects: General Anaesthetic

abstract: General anaesthetics cause widespread neurochemical and physiological changes in the brain. However, the precise mechanism of amnesic action is largely unknown. Gene expression changes in the hippocampus have been a focal point for investigation in this area, while effects on the cerebral cortex have been largely underreported even though the cerebral cortex has been shown to play a large role in memory consolidation and storage. Amnesia is likely due to the change in expression of memory-related genes within the neocortex or hippocampus of the mammalian brain. 

The first aim of this research was to investigate the in vitro cortical gene expression pattern of two memory-related genes; activity-regulated cytoskeleton-associated protein (Arc) and brain-derived neurotrophic factor (Bdnf), after a t=4 hour exposure to propofol- or sevoflurane- induced anaesthesia using an adult mouse brain slice model and real-time quantitative PCR. Five animals were used for each anaesthetic and a t=0 hour control, t=4 hour control and t=4 hour treated 400 μm slice were taken from each animal. Seizure-like activity was recorded from the brain slices to ensure viability of the tissue before carrying out the anaesthetic exposure. RNA was extracted, DNAse-treated, and then converted to cDNA. Quantitative PCR was then carried out to analyse Bdnf and Arc expression differences between the t=4 hour control and t=4 hour treated samples using Gapdh, β2m, Actb and HRPT1 as reference genes. The second research aim was to determine the Bdnf protein expression level and localisation after a t=4 hour exposure to propofol using western blot and immunohistochemistry methodologies.

Our research demonstrated that Arc was significantly down-regulated after exposure to sevoflurane for t=4 hours (p<0.05). Arc was also shown to be up-regulated after a t=4 hour exposure to propofol while Bdnf showed a downregulation to sevoflurane but an upregulation to propofol, however this data was not statistically significant. Western blot data showed that the rabbit polyclonal Bdnf antibody was binding to an off-target epitope at 55 kDa with mouse brain, heart, lung, liver, spleen and kidney whole tissue lysate. A newly sourced commercial Bdnf antibody was validated and western blot data showed recognition of the Bdnf epitope at the correct predicted size of 28 kDa in the mouse brain, heart and kidney. Immunohistochemistry of frozen mouse brain sections failed to produce a positive signal for two different Bdnf antibodies due to encountering technical issues. Hematoxylin and Eosin staining showed the tissue was still intact after the sectioning procedure. These issues could be resolved in the future by improving the tissue fixation length and optimising the antigen retrieval step. 

Eleven recommendations have been made to provide further insight into the gene expression levels of memory-related genes in the mouse brain. This includes varying the induction and maintenance of the two anaesthetic drugs, role of epigenetic modification, evaluating the mental state of the mice (depression), investigating Bdnf knockout, differential expression between areas of the brain and brain electrical activity. In addition, the gene expression pattern after a period of anaesthetic exposure should also be analysed. This may help elucidate the causes of postoperative cognitive dysfunction. Whole transcriptome analysis using RNA-Seq could be used to determine the expression levels of other known memory-related genes after anaesthetic exposure.

text: General Anaesthetic Modulation of Memory-Related Gene Expression Using an In vitro Brain Slice Model General anaesthetics cause widespread neurochemical and physiological changes in the brain. However, the precise mechanism of amnesic action is largely unknown. Gene expression changes in the hippocampus have been a focal point for investigation in this area, while effects on the cerebral cortex have been largely underreported even though the cerebral cortex has been shown to play a large role in memory consolidation and storage. Amnesia is likely due to the change in expression of memory-related genes within the neocortex or hippocampus of the mammalian brain. 

The first aim of this research was to investigate the in vitro cortical gene expression pattern of two memory-related genes; activity-regulated cytoskeleton-associated protein (Arc) and brain-derived neurotrophic factor (Bdnf), after a t=4 hour exposure to propofol- or sevoflurane- induced anaesthesia using an adult mouse brain slice model and real-time quantitative PCR. Five animals were used for each anaesthetic and a t=0 hour control, t=4 hour control and t=4 hour treated 400 μm slice were taken from each animal. Seizure-like activity was recorded from the brain slices to ensure viability of the tissue before carrying out the anaesthetic exposure. RNA was extracted, DNAse-treated, and then converted to cDNA. Quantitative PCR was then carried out to analyse Bdnf and Arc expression differences between the t=4 hour control and t=4 hour treated samples using Gapdh, β2m, Actb and HRPT1 as reference genes. The second research aim was to determine the Bdnf protein expression level and localisation after a t=4 hour exposure to propofol using western blot and immunohistochemistry methodologies.

Our research demonstrated that Arc was significantly down-regulated after exposure to sevoflurane for t=4 hours (p<0.05). Arc was also shown to be up-regulated after a t=4 hour exposure to propofol while Bdnf showed a downregulation to sevoflurane but an upregulation to propofol, however this data was not statistically significant. Western blot data showed that the rabbit polyclonal Bdnf antibody was binding to an off-target epitope at 55 kDa with mouse brain, heart, lung, liver, spleen and kidney whole tissue lysate. A newly sourced commercial Bdnf antibody was validated and western blot data showed recognition of the Bdnf epitope at the correct predicted size of 28 kDa in the mouse brain, heart and kidney. Immunohistochemistry of frozen mouse brain sections failed to produce a positive signal for two different Bdnf antibodies due to encountering technical issues. Hematoxylin and Eosin staining showed the tissue was still intact after the sectioning procedure. These issues could be resolved in the future by improving the tissue fixation length and optimising the antigen retrieval step. 

Eleven recommendations have been made to provide further insight into the gene expression levels of memory-related genes in the mouse brain. This includes varying the induction and maintenance of the two anaesthetic drugs, role of epigenetic modification, evaluating the mental state of the mice (depression), investigating Bdnf knockout, differential expression between areas of the brain and brain electrical activity. In addition, the gene expression pattern after a period of anaesthetic exposure should also be analysed. This may help elucidate the causes of postoperative cognitive dysfunction. Whole transcriptome analysis using RNA-Seq could be used to determine the expression levels of other known memory-related genes after anaesthetic exposure.

year: 2016

title: The contested shift to a bicultural understanding of place heritage in Aotearoa New Zealand

authors: Passey, Emma

abstract: This article explores contemporary threads in the heritage history of Aotearoa New Zealand, as we witness in real-time the emergence of alternative narratives of Indigenous heritage, in contrast to those of Western dominated modernity and colonial hegemony. Moves to actively decolonise heritage studies is creating shifts in the bicultural understanding of culture and heritage to overtly include place heritage. As this new framing emerges, wider acknowledgement of the significance of Māori heritage landscapes is growing. This is explored here in a series of four case-studies. Each account illustrates how Indigenous Māori voices have gained momentum, and reinforces how Aotearoa New Zealand is transitioning to greater bicultural appreciation through inclusion, changes in social and cultural conventions, and new interpretations of dominant narratives using tools like critical heritage studies and existing legal conventions. This evolution of heritage values is not without repeated contestation, however. Positively, increasing numbers of settler-Pākehā (non-Māori) as well as those colonised, no longer believe or accept the histories previously told. Much needed, robust, and sometimes difficult discussions are taking place, enabling New Zealanders to reconsider the significance of what heritage means to all its people.

text: The contested shift to a bicultural understanding of place heritage in Aotearoa New Zealand This article explores contemporary threads in the heritage history of Aotearoa New Zealand, as we witness in real-time the emergence of alternative narratives of Indigenous heritage, in contrast to those of Western dominated modernity and colonial hegemony. Moves to actively decolonise heritage studies is creating shifts in the bicultural understanding of culture and heritage to overtly include place heritage. As this new framing emerges, wider acknowledgement of the significance of Māori heritage landscapes is growing. This is explored here in a series of four case-studies. Each account illustrates how Indigenous Māori voices have gained momentum, and reinforces how Aotearoa New Zealand is transitioning to greater bicultural appreciation through inclusion, changes in social and cultural conventions, and new interpretations of dominant narratives using tools like critical heritage studies and existing legal conventions. This evolution of heritage values is not without repeated contestation, however. Positively, increasing numbers of settler-Pākehā (non-Māori) as well as those colonised, no longer believe or accept the histories previously told. Much needed, robust, and sometimes difficult discussions are taking place, enabling New Zealanders to reconsider the significance of what heritage means to all its people.

year: 2023-01-01