title: A new intrinsic thermal parameter for enzymes reveals true temperature optima

authors: Peterson, Michelle E.

subjects: enzyme

abstract: Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity and stability properties of five different enzymes with theoretical models. The results provide evidence for a new and fundamental third thermal parameter of enzymes, Teq, arising from a subsecond timescale-reversible temperature-dependent equilibrium between the active enzyme and an inactive (or less active) form. Thus, at temperatures above its optimum, the decrease in enzyme activity arising from the temperature-dependent shift in this equilibrium is up to two orders of magnitude greater than what occurs through thermal denaturation. This parameter has important implications for our understanding of the connection between catalytic activity and thermostability and of the effect of temperature on enzyme reactions within the cell. Unlike the Arrhenius activation energy, which is unaffected by the source (“evolved”) temperature of the enzyme, and enzyme stability, which is not necessarily related to activity, Teq is central to the physiological adaptation of an enzyme to its environmental temperature and links the molecular, physiological, and environmental aspects of the adaptation of life to temperature in a way that has not been described previously. We may therefore expect the effect of evolution on Teq with respect to enzyme temperature/activity effects to be more important than on thermal stability. Teq is also an important parameter to consider when engineering enzymes to modify their thermal properties by both rational design and by directed enzyme evolution.

text: A new intrinsic thermal parameter for enzymes reveals true temperature optima Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity and stability properties of five different enzymes with theoretical models. The results provide evidence for a new and fundamental third thermal parameter of enzymes, Teq, arising from a subsecond timescale-reversible temperature-dependent equilibrium between the active enzyme and an inactive (or less active) form. Thus, at temperatures above its optimum, the decrease in enzyme activity arising from the temperature-dependent shift in this equilibrium is up to two orders of magnitude greater than what occurs through thermal denaturation. This parameter has important implications for our understanding of the connection between catalytic activity and thermostability and of the effect of temperature on enzyme reactions within the cell. Unlike the Arrhenius activation energy, which is unaffected by the source (“evolved”) temperature of the enzyme, and enzyme stability, which is not necessarily related to activity, Teq is central to the physiological adaptation of an enzyme to its environmental temperature and links the molecular, physiological, and environmental aspects of the adaptation of life to temperature in a way that has not been described previously. We may therefore expect the effect of evolution on Teq with respect to enzyme temperature/activity effects to be more important than on thermal stability. Teq is also an important parameter to consider when engineering enzymes to modify their thermal properties by both rational design and by directed enzyme evolution.

year: 2004

title: The integration of heat pumps with biomass boiler flue gas for generating hot water

authors: Muldoon, Catherine

subjects: Biomass boiler

abstract: Achieving the goal of net zero greenhouse gases by 2050 requires reducing demand and switching from fossil fuels to renewables. Process heat in New Zealand is a significant source of greenhouse gas emissions. Transitioning to biomass boilers could lower emissions and offer opportunities for waste heat recovery by integrating heat pumps with the flue gas.
This thesis evaluates heat pump integration for hot water production, supplying low temperature process heat. Using an industrial case study, a boiler model is developed and confirmed against industrial measurements. Flue gas composition is modelled to determine temperature-enthalpy profiles, and therefore the heat available for recovery and upgrade. Combustion is modelled for different biomass fuels and varying flue gas oxygen levels. The higher moisture fuels showed increased availability of heat for utilisation, which decreased as flue gas oxygen levels increased.
Three heat pump cycles are explored to maximise efficiency. Refrigerant selection is considered with respect to environmental factors and thermodynamic suitability. Using a basic cycle heat pump the three most promising refrigerants, ammonia, propane and cyclopropane, were identified and considered in more detail for the industrial case. Subcooling improved efficiency and should be maximised. An internal heat exchanger also improves efficiency and reliability and should be considered. Two zeotropic mixtures were investigated to take advantage of the temperature glide. The propane-pentane (50wt%-50wt%) blend matched the temperature profile more closely and provided increases in COP.
To make the most of the latent heat in the flue gas, the evaporator temperature must be much lower than the dew point. However, absorbing heat from low source temperatures results in lower COPs, but higher volumes of hot water generated.

text: The integration of heat pumps with biomass boiler flue gas for generating hot water Achieving the goal of net zero greenhouse gases by 2050 requires reducing demand and switching from fossil fuels to renewables. Process heat in New Zealand is a significant source of greenhouse gas emissions. Transitioning to biomass boilers could lower emissions and offer opportunities for waste heat recovery by integrating heat pumps with the flue gas.
This thesis evaluates heat pump integration for hot water production, supplying low temperature process heat. Using an industrial case study, a boiler model is developed and confirmed against industrial measurements. Flue gas composition is modelled to determine temperature-enthalpy profiles, and therefore the heat available for recovery and upgrade. Combustion is modelled for different biomass fuels and varying flue gas oxygen levels. The higher moisture fuels showed increased availability of heat for utilisation, which decreased as flue gas oxygen levels increased.
Three heat pump cycles are explored to maximise efficiency. Refrigerant selection is considered with respect to environmental factors and thermodynamic suitability. Using a basic cycle heat pump the three most promising refrigerants, ammonia, propane and cyclopropane, were identified and considered in more detail for the industrial case. Subcooling improved efficiency and should be maximised. An internal heat exchanger also improves efficiency and reliability and should be considered. Two zeotropic mixtures were investigated to take advantage of the temperature glide. The propane-pentane (50wt%-50wt%) blend matched the temperature profile more closely and provided increases in COP.
To make the most of the latent heat in the flue gas, the evaporator temperature must be much lower than the dew point. However, absorbing heat from low source temperatures results in lower COPs, but higher volumes of hot water generated.

year: 2024

title: An Interactive User Management System for Multilingual Documents: A case study of the Pei Jones collection

authors: Cader, Papitha

subjects: Greenstone Digital Library

abstract: Can general purpose DL software architecture be designed to provide a flexible environment that supports both the management and end-user roles through a single unified web interface? In particular we take the collected correspondence of the Māori scholar Dr. Pei Jones and undertake case study of how this approach can be applied in a practical way.

text: An Interactive User Management System for Multilingual Documents: A case study of the Pei Jones collection Can general purpose DL software architecture be designed to provide a flexible environment that supports both the management and end-user roles through a single unified web interface? In particular we take the collected correspondence of the Māori scholar Dr. Pei Jones and undertake case study of how this approach can be applied in a practical way.

year: 2012