title: Convenient large-scale purification of yessotoxin from Protoceratium reticulatum culture and isolation of a novel furanoyessotoxin

authors: Loader, Jared I.

subjects: chemistry

abstract: Yessotoxins from a large-scale culture (226 L) of Protoceratium reticulatum strain CAWD129 were harvested by filtration followed by solid-phase extraction. The extract was purified by column chromatography over basic alumina and reverse-phase flash chromatography to afford pure yessotoxin (193 mg). Isolation of yessotoxin was greatly facilitated by selection of a strain which did not produce analogues that interfered with yessotoxin isolation. In addition to yessotoxin, numerous minor yessotoxins were detected by LC-MS in other fractions. From one of these, an early eluting minor analogue with the same molecular weight as yessotoxin and a similar mass spectrometric fragmentation pattern was isolated. This analogue was identified by NMR and mass spectrometry as a novel yessotoxin analogue containing a furan ring in the side chain. This finding reveals biosynthetic flexibility of the yessotoxin pathway in P. reticulatum and confirms earlier findings of production of many minor yessotoxin analogues by this alga. Production of these analogues appeared to be a constitutive trait of P. reticulatum CAWD129.

text: Convenient large-scale purification of yessotoxin from Protoceratium reticulatum culture and isolation of a novel furanoyessotoxin Yessotoxins from a large-scale culture (226 L) of Protoceratium reticulatum strain CAWD129 were harvested by filtration followed by solid-phase extraction. The extract was purified by column chromatography over basic alumina and reverse-phase flash chromatography to afford pure yessotoxin (193 mg). Isolation of yessotoxin was greatly facilitated by selection of a strain which did not produce analogues that interfered with yessotoxin isolation. In addition to yessotoxin, numerous minor yessotoxins were detected by LC-MS in other fractions. From one of these, an early eluting minor analogue with the same molecular weight as yessotoxin and a similar mass spectrometric fragmentation pattern was isolated. This analogue was identified by NMR and mass spectrometry as a novel yessotoxin analogue containing a furan ring in the side chain. This finding reveals biosynthetic flexibility of the yessotoxin pathway in P. reticulatum and confirms earlier findings of production of many minor yessotoxin analogues by this alga. Production of these analogues appeared to be a constitutive trait of P. reticulatum CAWD129.

year: 2007

title: Draft Genome Sequence of Venenivibrio stagnispumantis CP.B2T, Isolated from Champagne Pool, Waiotapu, Aotearoa-New Zealand

authors: Power, Jean Florence

abstract: Venenivibrio stagnispumantis strain CP.B2T is a thermophilic, chemolithoautotrophic bacterium from the family Hydrogenothermaceae (phylum Aquificota), isolated from Champagne Pool in the Waiotapu geothermal field, Aotearoa-New Zealand. The genome consists of 1.73 Mbp in 451 contigs with a 30.8 mol% G+C content.

text: Draft Genome Sequence of Venenivibrio stagnispumantis CP.B2T, Isolated from Champagne Pool, Waiotapu, Aotearoa-New Zealand Venenivibrio stagnispumantis strain CP.B2T is a thermophilic, chemolithoautotrophic bacterium from the family Hydrogenothermaceae (phylum Aquificota), isolated from Champagne Pool in the Waiotapu geothermal field, Aotearoa-New Zealand. The genome consists of 1.73 Mbp in 451 contigs with a 30.8 mol% G+C content.

year: 2023-02-16

title: In service provision for teachers of Maori language and teachers who teach through the medium of Maori: A working model reviewed

authors: Johnson, Diane

subjects: te reo Māori

abstract: This paper reports on the design, administration and effectiveness of an in-service programme designed by the University of Waikato in partnership with the New Zealand Ministry of Education. The programme aims to: improve language proficiency; assist with the interpretation and implementation of Māori curriculum statements; address issues involved in teaching Māori and teaching through the medium of Māori; assist with lesson planning and lesson delivery, materials development and testing and assessment.

text: In service provision for teachers of Maori language and teachers who teach through the medium of Maori: A working model reviewed This paper reports on the design, administration and effectiveness of an in-service programme designed by the University of Waikato in partnership with the New Zealand Ministry of Education. The programme aims to: improve language proficiency; assist with the interpretation and implementation of Māori curriculum statements; address issues involved in teaching Māori and teaching through the medium of Māori; assist with lesson planning and lesson delivery, materials development and testing and assessment.

year: 2001-02