title: Proteomes and Signalling Pathways of Antler Stem Cells

authors: Li, Chunyi

abstract: As the only known example of complete organ regeneration in mammals, deer antler in the growing or velvet phase is of major interest in developmental biology. This regeneration event initiates from self-renewing antler stem cells that exhibit pluripotency. At present, it remains unclear how the activation and quiescence of antler stem cells are regulated. Therefore, in the present study proteins that were differentially expressed between the antler stem cells and somatic cells (facial periosteum) were identified by a gel-based proteomic technique, and analysed using Ingenuity Pathway Analysis software. Several molecular pathways (PI3K/Akt, ERK/MAPK, p38 MAPK, etc.) were found to be activated during proliferation. Also expressed were the transcription factors POU5F1, SOX2, NANOG and MYC, which are key markers of embryonic stem cells. Expression of these proteins was confirmed in both cultured cells and fresh tissues by Western blot analysis. Therefore, the molecular pathways and transcription factors identified in the current study are common to embryonic and adult stem cells. However, expression of embryonic stem cell transcription factors would suggest that antler stem cells are, potentially, an intermediary stem cell type between embryonic and the more specialized tissue-specific stem cells like those residing in muscle, fat or from a hematopoietic origin. The retention of this embryonic, pluripotent lineage may be of fundamental importance for the subsequent regenerative capacity of antlers.

text: Proteomes and Signalling Pathways of Antler Stem Cells As the only known example of complete organ regeneration in mammals, deer antler in the growing or velvet phase is of major interest in developmental biology. This regeneration event initiates from self-renewing antler stem cells that exhibit pluripotency. At present, it remains unclear how the activation and quiescence of antler stem cells are regulated. Therefore, in the present study proteins that were differentially expressed between the antler stem cells and somatic cells (facial periosteum) were identified by a gel-based proteomic technique, and analysed using Ingenuity Pathway Analysis software. Several molecular pathways (PI3K/Akt, ERK/MAPK, p38 MAPK, etc.) were found to be activated during proliferation. Also expressed were the transcription factors POU5F1, SOX2, NANOG and MYC, which are key markers of embryonic stem cells. Expression of these proteins was confirmed in both cultured cells and fresh tissues by Western blot analysis. Therefore, the molecular pathways and transcription factors identified in the current study are common to embryonic and adult stem cells. However, expression of embryonic stem cell transcription factors would suggest that antler stem cells are, potentially, an intermediary stem cell type between embryonic and the more specialized tissue-specific stem cells like those residing in muscle, fat or from a hematopoietic origin. The retention of this embryonic, pluripotent lineage may be of fundamental importance for the subsequent regenerative capacity of antlers.

year: 2012

title: The relationship between well-being and wildfire

authors: Kaval, Pamela

subjects: well-being evaluation method

abstract: In this study, the well-being evaluation method, a technique for measuring individual utility, was used to study how people in the wildland urban interface of Colorado (USA) felt about their lives before and after two hypothetical wildfire scenarios. Variables such as age, family size, fire frequency, and property value were found to affect initial well-being levels. However, if a wildfire were to occur, many variables that initially affected well-being were no longer significant. It was found that after wildfire, the frequency of wildfire occurrence became the most important influence on well-being. These results have several implications for wildfire managers. First, the well-being of Colorado wildland urban interface residents would be enhanced by a reduction in the frequency of high-intensity wildfires. Secondly, an extremely high percentage of respondents were in favor of prescribed burning. Therefore, the reduction of high-intensity fires could not only be accomplished by conducting a rotation of prescribed fires, but that prescribed burning would be accepted by the public living in the wildland urban interface.

text: The relationship between well-being and wildfire In this study, the well-being evaluation method, a technique for measuring individual utility, was used to study how people in the wildland urban interface of Colorado (USA) felt about their lives before and after two hypothetical wildfire scenarios. Variables such as age, family size, fire frequency, and property value were found to affect initial well-being levels. However, if a wildfire were to occur, many variables that initially affected well-being were no longer significant. It was found that after wildfire, the frequency of wildfire occurrence became the most important influence on well-being. These results have several implications for wildfire managers. First, the well-being of Colorado wildland urban interface residents would be enhanced by a reduction in the frequency of high-intensity wildfires. Secondly, an extremely high percentage of respondents were in favor of prescribed burning. Therefore, the reduction of high-intensity fires could not only be accomplished by conducting a rotation of prescribed fires, but that prescribed burning would be accepted by the public living in the wildland urban interface.

year: 2006-10

title: Understanding ‘higher’ Māori fertility in a ‘low’ fertility context: Does cultural identity make a difference?

authors: Rarere, Moana

subjects: Maori fertility

abstract: The Māori fertility transition brought an end to decades of very high fertility rates, and a convergence towards long-term fertility levels similar to Pākehā/New Zealand European women. However, important differences endure. The Māori total fertility rate (TFR) remains above replacement level, and Māori women have children earlier and over a longer period. All of this has and still is occurring in a society that facilitates and favours low fertility and small family sizes. Using births data and cultural identity markers in the New Zealand Census, this paper explores the influence of culture as a contributing factor to higher fertility outcomes amongst Māori women in a low-fertility society.

text: Understanding ‘higher’ Māori fertility in a ‘low’ fertility context: Does cultural identity make a difference? The Māori fertility transition brought an end to decades of very high fertility rates, and a convergence towards long-term fertility levels similar to Pākehā/New Zealand European women. However, important differences endure. The Māori total fertility rate (TFR) remains above replacement level, and Māori women have children earlier and over a longer period. All of this has and still is occurring in a society that facilitates and favours low fertility and small family sizes. Using births data and cultural identity markers in the New Zealand Census, this paper explores the influence of culture as a contributing factor to higher fertility outcomes amongst Māori women in a low-fertility society.

year: 2018