title: The orphan G protein-coupled receptor gene GPR178 is evolutionary conserved and altered in response to acute changes in food intake

authors: Caruso, Vanni

abstract: G protein-coupled receptors (GPCRs) are a class of integral membrane proteins mediating physiological functions fundamental for survival, including energy homeostasis. A few years ago, an amino acid sequence of a novel GPCR gene was identified and named GPR178. In this study, we provide new insights regarding the biological significance of Gpr178 protein, investigating its evolutionary history and tissue distribution as well as examining the relationship between its expression level and feeding status. Our phylogenetic analysis indicated that GPR178 is highly conserved among all animal species investigated, and that GPR178 is not a member of a protein family. Real-time PCR and in situ hybridization revealed wide expression of Gpr178 mRNA in both the brain and periphery, with high expression density in the hypothalamus and brainstem, areas involved in the regulation of food intake. Hence, changes in receptor expression were assessed following several feeding paradigms including starvation and overfeeding. Short-term starvation (12-48h) or food restriction resulted in upregulation of Gpr178 mRNA expression in the brainstem, hypothalamus and prefrontal cortex. Conversely, short-term (48h) exposure to sucrose or Intralipid solutions downregulated Gpr178 mRNA in the brainstem; long-term exposure (10 days) to a palatable high-fat and high-sugar diet resulted in a downregulation of Gpr178 in the amygdala but not in the hypothalamus. Our results indicate that hypothalamic Gpr178 gene expression is altered during acute exposure to starvation or acute exposure to palatable food. Changes in gene expression following palatable diet consumption suggest a possible involvement of Gpr178 in the complex mechanisms of feeding reward.

text: The orphan G protein-coupled receptor gene GPR178 is evolutionary conserved and altered in response to acute changes in food intake G protein-coupled receptors (GPCRs) are a class of integral membrane proteins mediating physiological functions fundamental for survival, including energy homeostasis. A few years ago, an amino acid sequence of a novel GPCR gene was identified and named GPR178. In this study, we provide new insights regarding the biological significance of Gpr178 protein, investigating its evolutionary history and tissue distribution as well as examining the relationship between its expression level and feeding status. Our phylogenetic analysis indicated that GPR178 is highly conserved among all animal species investigated, and that GPR178 is not a member of a protein family. Real-time PCR and in situ hybridization revealed wide expression of Gpr178 mRNA in both the brain and periphery, with high expression density in the hypothalamus and brainstem, areas involved in the regulation of food intake. Hence, changes in receptor expression were assessed following several feeding paradigms including starvation and overfeeding. Short-term starvation (12-48h) or food restriction resulted in upregulation of Gpr178 mRNA expression in the brainstem, hypothalamus and prefrontal cortex. Conversely, short-term (48h) exposure to sucrose or Intralipid solutions downregulated Gpr178 mRNA in the brainstem; long-term exposure (10 days) to a palatable high-fat and high-sugar diet resulted in a downregulation of Gpr178 in the amygdala but not in the hypothalamus. Our results indicate that hypothalamic Gpr178 gene expression is altered during acute exposure to starvation or acute exposure to palatable food. Changes in gene expression following palatable diet consumption suggest a possible involvement of Gpr178 in the complex mechanisms of feeding reward.

year: 2015-06-05

title: Researching poverty to make a difference: The need for reciprocity and advocacy in community research

authors: Hodgetts, Darrin

subjects: psychology

abstract: Growth in poverty throughout the Organisation for Economic Co-operation and Development [OECD] hurts people. The Auckland City Mission Family100 project explores the everyday lives, frustrations and dilemmas faced by 100 families living in poverty in Auckland. This article reflects on poverty in New Zealand, associated welfare ‘reforms’, the consequences of recent change in exacerbating hardship, and our own efforts to advocate for the rights of beneficiaries. Specific attention is given to a workshop run by the research team with the judiciary, and what such activities foreground in terms of the relational nature of research, reciprocity and advocacy.

text: Researching poverty to make a difference: The need for reciprocity and advocacy in community research Growth in poverty throughout the Organisation for Economic Co-operation and Development [OECD] hurts people. The Auckland City Mission Family100 project explores the everyday lives, frustrations and dilemmas faced by 100 families living in poverty in Auckland. This article reflects on poverty in New Zealand, associated welfare ‘reforms’, the consequences of recent change in exacerbating hardship, and our own efforts to advocate for the rights of beneficiaries. Specific attention is given to a workshop run by the research team with the judiciary, and what such activities foreground in terms of the relational nature of research, reciprocity and advocacy.

year: 2013

title: Developing restorative practices in schools: Some reflections

authors: Drewery, Wendy

subjects: Counselling

abstract: The use of disciplinary practices derived from restorative justice have recently been gaining popularity and inciting keen interest in the education community. Practices that have so far been introduced have tended to centre on conferencing, although there is a broadening range of other practices in schools that are being brought under the heading “restorative”. This paper offers some reflections on these developments, building on the experiences of a team at the University of Waikato, which completed two projects on restorative conferencing in schools for the Ministry of Education under the rubric of the Suspension Reduction Initiative, and continues to develop understanding of the practices. The projects included developing and trialling processes for suspension hearings using restorative conferencing and principles from restorative justice. Objectives of both projects were related to the desire to reduce numbers of suspensions and exclusions, particularly of Māori children. We argue that the introduction of restorative practices invites schools into some tectonic shifts in thinking about offending behaviour, about community, and ultimately about the purposes of education.

text: Developing restorative practices in schools: Some reflections The use of disciplinary practices derived from restorative justice have recently been gaining popularity and inciting keen interest in the education community. Practices that have so far been introduced have tended to centre on conferencing, although there is a broadening range of other practices in schools that are being brought under the heading “restorative”. This paper offers some reflections on these developments, building on the experiences of a team at the University of Waikato, which completed two projects on restorative conferencing in schools for the Ministry of Education under the rubric of the Suspension Reduction Initiative, and continues to develop understanding of the practices. The projects included developing and trialling processes for suspension hearings using restorative conferencing and principles from restorative justice. Objectives of both projects were related to the desire to reduce numbers of suspensions and exclusions, particularly of Māori children. We argue that the introduction of restorative practices invites schools into some tectonic shifts in thinking about offending behaviour, about community, and ultimately about the purposes of education.

year: 2005