title: Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheep

authors: Jacobson, Gregory M.

subjects: Antigen design

abstract: Antigen-specific polyclonal immunoglobulins derived from the serum, colostrum, or milk of immunized ruminant animals have potential as scalable therapeutics for the control of viral diseases including COVID-19. Here we show that the immunization of sheep with fusions of the SARS-CoV-2 receptor binding domain (RBD) to ovine IgG2a Fc domains promotes significantly higher levels of antigen-specific antibodies compared to native RBD or full-length spike antigens. This antibody population contained elevated levels of neutralizing antibodies that suppressed binding between the RBD and hACE2 receptors in vitro. A second immune-stimulating fusion candidate, Granulocyte-macrophage colony-stimulating factor (GM-CSF), induced high neutralizing responses in select animals but narrowly missed achieving significance. We further demonstrated that the antibodies induced by these fusion antigens were transferred into colostrum/milk and possessed cross-neutralizing activity against diverse SARS-CoV-2 variants. Our findings highlight a new pathway for recombinant antigen design in ruminant animals with applications in immune milk production and animal health.

text: Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheep Antigen-specific polyclonal immunoglobulins derived from the serum, colostrum, or milk of immunized ruminant animals have potential as scalable therapeutics for the control of viral diseases including COVID-19. Here we show that the immunization of sheep with fusions of the SARS-CoV-2 receptor binding domain (RBD) to ovine IgG2a Fc domains promotes significantly higher levels of antigen-specific antibodies compared to native RBD or full-length spike antigens. This antibody population contained elevated levels of neutralizing antibodies that suppressed binding between the RBD and hACE2 receptors in vitro. A second immune-stimulating fusion candidate, Granulocyte-macrophage colony-stimulating factor (GM-CSF), induced high neutralizing responses in select animals but narrowly missed achieving significance. We further demonstrated that the antibodies induced by these fusion antigens were transferred into colostrum/milk and possessed cross-neutralizing activity against diverse SARS-CoV-2 variants. Our findings highlight a new pathway for recombinant antigen design in ruminant animals with applications in immune milk production and animal health.

year: 2023-06

title: Organisational learning and development: A Vanuatu educational perspective.

authors: Tarosa, Gayleen

subjects: Vanuatu

abstract: Organisational learning within an educational context is a complex and multidimensional process. This paper reports on a study that investigates organisational learning during the process of development of Vanuatu’s nationalised English medium secondary school assessment process, a significant educational change in Vanuatu. This qualitative inquiry reviewed and critiqued the organisational learning that resulted from a pilot programme leading to the change, and involved participants from a number of levels within the Vanuatu education system. The study identified several factors that may have hindered the planning process. A revised change management framework for the Vanuatu education system is proposed, as a result of the critique, and recommendations towards improving the implementation of future educational changes in Vanuatu are made. The resulting framework and recommendations may assist other Pacific countries engaged in similar senior assessment nationalisation, and, also contribute more generally to others involved in systemic educational change processes.

text: Organisational learning and development: A Vanuatu educational perspective. Organisational learning within an educational context is a complex and multidimensional process. This paper reports on a study that investigates organisational learning during the process of development of Vanuatu’s nationalised English medium secondary school assessment process, a significant educational change in Vanuatu. This qualitative inquiry reviewed and critiqued the organisational learning that resulted from a pilot programme leading to the change, and involved participants from a number of levels within the Vanuatu education system. The study identified several factors that may have hindered the planning process. A revised change management framework for the Vanuatu education system is proposed, as a result of the critique, and recommendations towards improving the implementation of future educational changes in Vanuatu are made. The resulting framework and recommendations may assist other Pacific countries engaged in similar senior assessment nationalisation, and, also contribute more generally to others involved in systemic educational change processes.

year: 2016

title: Urban Rural Differences in Breast Cancer in New Zealand

authors: Lawrenson, Ross

subjects: Science & Technology

abstract: Many rural communities have poor access to health services due to a combination of distance from specialist services and a relative shortage of general practitioners. Our aims were to compare the characteristics of urban and rural women with breast cancer in New Zealand, to assess breast cancer-specific and all-cause survival using the Kaplan–Meier method and Cox proportional hazards model, and to assess whether the impact of rurality is different for Māori and New Zealand (NZ) European women. We found that rural women tended to be older and were more likely to be Māori. Overall there were no differences between urban and rural women with regards their survival. Rural Māori tended to be older, more likely to be diagnosed with metastatic disease and less likely to be screen detected than urban Māori. Rural Māori women had inferior breast cancer-specific survival and all-cause survival at 10 years at 72.1% and 55.8% compared to 77.9% and 64.9% for urban Māori. The study shows that rather than being concerned that more needs to be done for rural women in general it is rural Māori women where we need to make extra efforts to ensure early stage at diagnosis and optimum treatment.

text: Urban Rural Differences in Breast Cancer in New Zealand Many rural communities have poor access to health services due to a combination of distance from specialist services and a relative shortage of general practitioners. Our aims were to compare the characteristics of urban and rural women with breast cancer in New Zealand, to assess breast cancer-specific and all-cause survival using the Kaplan–Meier method and Cox proportional hazards model, and to assess whether the impact of rurality is different for Māori and New Zealand (NZ) European women. We found that rural women tended to be older and were more likely to be Māori. Overall there were no differences between urban and rural women with regards their survival. Rural Māori tended to be older, more likely to be diagnosed with metastatic disease and less likely to be screen detected than urban Māori. Rural Māori women had inferior breast cancer-specific survival and all-cause survival at 10 years at 72.1% and 55.8% compared to 77.9% and 64.9% for urban Māori. The study shows that rather than being concerned that more needs to be done for rural women in general it is rural Māori women where we need to make extra efforts to ensure early stage at diagnosis and optimum treatment.

year: 2016