title: Citrate synthases from the Archaea: Development of a bio-specific, affinity chromatography purification procedure

authors: James, Keith D.

subjects: archaea

abstract: Citrate synthases from both thermophilic and halophilic Archaea have been purified to homogeneity using affinity chromatography on Matrex Gel Red A and elution with a combination of substrate (oxaloacetate) and product (coenzyme A). In a number of cases, purification from cell-extract to protein suitable for N-terminal sequencing can be achieved by this single-step procedure. The method is particularly useful in the rapid purification of a thermophilic archaeal citrate synthase from a cloned gene expressed in a mesophilic host.

text: Citrate synthases from the Archaea: Development of a bio-specific, affinity chromatography purification procedure Citrate synthases from both thermophilic and halophilic Archaea have been purified to homogeneity using affinity chromatography on Matrex Gel Red A and elution with a combination of substrate (oxaloacetate) and product (coenzyme A). In a number of cases, purification from cell-extract to protein suitable for N-terminal sequencing can be achieved by this single-step procedure. The method is particularly useful in the rapid purification of a thermophilic archaeal citrate synthase from a cloned gene expressed in a mesophilic host.

year: 1994

title: Investigating Gravity and Electromagnetism on a 10 Dimensional Manifold with Local Symmetry so(2,3)

authors: Ussher, Matthew Neville

abstract: The Hawthorn model [1] is built upon the idea that the Lie algebra so(2, 3) is a more natural description of the local structure of space-time than the Poincare Lie algebra, with the former contracting to the latter in the limit of the contraction parameter r tending to infinity. This notion is explored in the context of a 10-dimensional space-time referred to as an ADS manifold. Here we build on the work of Crump [2] and try to incorporate field equations for gravity into the model. We derive two apparently different equations describing gravitational phenomena, demonstrate an intimate connection between gravity and electromagnetism and provide a first estimate as to the value of the contraction parameter r.

text: Investigating Gravity and Electromagnetism on a 10 Dimensional Manifold with Local Symmetry so(2,3) The Hawthorn model [1] is built upon the idea that the Lie algebra so(2, 3) is a more natural description of the local structure of space-time than the Poincare Lie algebra, with the former contracting to the latter in the limit of the contraction parameter r tending to infinity. This notion is explored in the context of a 10-dimensional space-time referred to as an ADS manifold. Here we build on the work of Crump [2] and try to incorporate field equations for gravity into the model. We derive two apparently different equations describing gravitational phenomena, demonstrate an intimate connection between gravity and electromagnetism and provide a first estimate as to the value of the contraction parameter r.

year: 2013

title: Media psychology, symbolic power and social justice in Aotearoa

authors: Hodgetts, Darrin

subjects: media

abstract: Psychologists reside in a world saturated by media. We work in professional contexts where guidelines for practice foreground ethical obligations to address issues of social justice. This paper addresses both these contextual dimensions of psychological research and practice. We explore the social significance of increased media production by Maori in challenging the tendency in mainstream media to marginalize Maori concerns while promoting Pakeha perspectives. The analysis focuses on the recent 'Inside Out documentary - Hikoi', which was initiated by two young Maori women as a challenge to media framing of Maori protests as 'unjustified' and 'disruptive' acts. We illustrate how this documentary furthers public dialogue regarding the foreshore and seabed controversy by promoting an alternative depiction of a Maori protest, which emphasize the history of grievances and social unity. The implications of such representations for psychologists working to address issues of social justice and to challenge abuses of symbolic power are discussed.

text: Media psychology, symbolic power and social justice in Aotearoa Psychologists reside in a world saturated by media. We work in professional contexts where guidelines for practice foreground ethical obligations to address issues of social justice. This paper addresses both these contextual dimensions of psychological research and practice. We explore the social significance of increased media production by Maori in challenging the tendency in mainstream media to marginalize Maori concerns while promoting Pakeha perspectives. The analysis focuses on the recent 'Inside Out documentary - Hikoi', which was initiated by two young Maori women as a challenge to media framing of Maori protests as 'unjustified' and 'disruptive' acts. We illustrate how this documentary furthers public dialogue regarding the foreshore and seabed controversy by promoting an alternative depiction of a Maori protest, which emphasize the history of grievances and social unity. The implications of such representations for psychologists working to address issues of social justice and to challenge abuses of symbolic power are discussed.

year: 2004-11-19