title: A preliminary conservation genetic study of Pittosporum obcordatum (Pittosporaceae), an endemic New Zealand species with a disjunct distribution

authors: Wright, Sarah A.

subjects: Genetic connectivity

abstract: Pittosporum obcordatum (Pittosporaceae; heart-leaved kōhūhū) is an endemic New Zealand plant species that is classified as Threatened - Nationally Vulnerable. It has a disjunct distribution and is only known from relatively few and small populations. Using 10 Inter-Simple Sequence Repeat markers (ISSRs), we studied patterns of genetic diversity and genetic differentiation among eight out of the c. 14 populations of this species to inform its conservation management. Pittosporum obcordatum has low genetic diversity at the population level (uHe = 0.169) compared to other long-lived and outcrossing species, but genetic diversity is relatively high in comparison with several other threatened species. Spearman’s Rank Correlation Coefficients suggest significant positive correlations between population size and genetic diversity as measured by the percentage of polymorphic loci and uHe. Pittosporum obcordatum also shows relatively high levels of genetic differentiation among populations (AMOVA-derived Φ’st = 0.508, P < 0.001; all pairwise Φst values P < 0.05), indicating low genetic connectivity. Populations with relatively few plants are therefore prone to further reductions in genetic diversity through inbreeding and genetic drift. Of these, especially the Kaitaia, Owen Valley and Paengaroa populations are of conservation concern, because they contain private alleles, and therefore notably contribute to the genetic diversity of P. obcordatum.

text: A preliminary conservation genetic study of Pittosporum obcordatum (Pittosporaceae), an endemic New Zealand species with a disjunct distribution Pittosporum obcordatum (Pittosporaceae; heart-leaved kōhūhū) is an endemic New Zealand plant species that is classified as Threatened - Nationally Vulnerable. It has a disjunct distribution and is only known from relatively few and small populations. Using 10 Inter-Simple Sequence Repeat markers (ISSRs), we studied patterns of genetic diversity and genetic differentiation among eight out of the c. 14 populations of this species to inform its conservation management. Pittosporum obcordatum has low genetic diversity at the population level (uHe = 0.169) compared to other long-lived and outcrossing species, but genetic diversity is relatively high in comparison with several other threatened species. Spearman’s Rank Correlation Coefficients suggest significant positive correlations between population size and genetic diversity as measured by the percentage of polymorphic loci and uHe. Pittosporum obcordatum also shows relatively high levels of genetic differentiation among populations (AMOVA-derived Φ’st = 0.508, P < 0.001; all pairwise Φst values P < 0.05), indicating low genetic connectivity. Populations with relatively few plants are therefore prone to further reductions in genetic diversity through inbreeding and genetic drift. Of these, especially the Kaitaia, Owen Valley and Paengaroa populations are of conservation concern, because they contain private alleles, and therefore notably contribute to the genetic diversity of P. obcordatum.

year: 2017

title: Thermally resolved synchrotron FT-IR microscopy of structural changes in bloodmeal-based thermoplastics

authors: Bier, James Michael

subjects: secondary structure estimation

abstract: Synchrotron FT-IR micro-spectroscopy was used to determine thermally induced structural changes in thermoplastic protein produced from bloodmeal after mixing with sodium sulphite, sodium dodecyl sulphate, urea, tri-ethylene glycol and water. Changes in protein secondary structure at elevated temperature were assessed using second derivative peak height ratios in the amide III region (1,200–1,330 cm⁻¹) and compared with DSC and DMA results over the same temperature range. The results show an increase in ordered β-sheet structures with temperature at the expense of random coils, and that these β-sheets do not melt in the temperature range up to extrusion temperature of 120 °C. The implication of this is that during melt processing, β-sheet clusters may remain intact, similar to dispersed particulate fillers.

text: Thermally resolved synchrotron FT-IR microscopy of structural changes in bloodmeal-based thermoplastics Synchrotron FT-IR micro-spectroscopy was used to determine thermally induced structural changes in thermoplastic protein produced from bloodmeal after mixing with sodium sulphite, sodium dodecyl sulphate, urea, tri-ethylene glycol and water. Changes in protein secondary structure at elevated temperature were assessed using second derivative peak height ratios in the amide III region (1,200–1,330 cm⁻¹) and compared with DSC and DMA results over the same temperature range. The results show an increase in ordered β-sheet structures with temperature at the expense of random coils, and that these β-sheets do not melt in the temperature range up to extrusion temperature of 120 °C. The implication of this is that during melt processing, β-sheet clusters may remain intact, similar to dispersed particulate fillers.

year: 2013

title: Āta whakarongo: Understanding whānau experiences of audiology early intervention

authors: Dargaville, Cynthia Michelle

subjects: Māori

abstract: For tamariki with hearing loss, early intervention is of critical importance in maximising speech and language, socio-emotional and academic development outcomes. Better hearing outcomes can be achieved by minimising diagnosis delays and initiating intervention treatment as soon as possible. Preliminary data from Waikato District Health Board indicated an equity gap at the point of diagnosis with persisting inequities for tamariki Māori during the delivery of early intervention services. High rates of appointment non-attendance within this group (27% Māori vs. 8% for non-Māori) are a cause for concern when approximately 50% of total appointments are for tamariki Māori. This research seeks to understand the experiences of Māori whānau accessing Waikato’s Audiology Early Intervention Service in order to identify changes the service can make to improve Māori engagement in the future. This study uses qualitative methodologies within a kaupapa Māori framework to investigate the access experiences of Māori whānau of tamariki identified with hearing loss. Interviews with six whānau from Hamilton city and rural Waikato provided rich narratives to inform changes to the Audiology Service to improve the hearing outcomes of tamariki Māori. Whānau shared systems and practical barriers that contributed to challenges with attending appointments including the location, appointment timing, travel and parking. From entering an unwelcoming waiting area, whānau described the added stress experienced to the depth of their emotional response to the hearing loss diagnosis. All shared positive interactions with staff and detailed what could have made their health experience better. After leaning into the kōrero and connecting to the whānau experience, a Manaaki-based pathway is proposed to support Māori ways of being, to ease whānau through the barriers and to combat the alienating experiences of engaging with the health system.

text: Āta whakarongo: Understanding whānau experiences of audiology early intervention For tamariki with hearing loss, early intervention is of critical importance in maximising speech and language, socio-emotional and academic development outcomes. Better hearing outcomes can be achieved by minimising diagnosis delays and initiating intervention treatment as soon as possible. Preliminary data from Waikato District Health Board indicated an equity gap at the point of diagnosis with persisting inequities for tamariki Māori during the delivery of early intervention services. High rates of appointment non-attendance within this group (27% Māori vs. 8% for non-Māori) are a cause for concern when approximately 50% of total appointments are for tamariki Māori. This research seeks to understand the experiences of Māori whānau accessing Waikato’s Audiology Early Intervention Service in order to identify changes the service can make to improve Māori engagement in the future. This study uses qualitative methodologies within a kaupapa Māori framework to investigate the access experiences of Māori whānau of tamariki identified with hearing loss. Interviews with six whānau from Hamilton city and rural Waikato provided rich narratives to inform changes to the Audiology Service to improve the hearing outcomes of tamariki Māori. Whānau shared systems and practical barriers that contributed to challenges with attending appointments including the location, appointment timing, travel and parking. From entering an unwelcoming waiting area, whānau described the added stress experienced to the depth of their emotional response to the hearing loss diagnosis. All shared positive interactions with staff and detailed what could have made their health experience better. After leaning into the kōrero and connecting to the whānau experience, a Manaaki-based pathway is proposed to support Māori ways of being, to ease whānau through the barriers and to combat the alienating experiences of engaging with the health system.

year: 2023