title: A RAPD-based comparison of thermophilic bacilli from milk powders

authors: Ronimus, Ron S.

subjects: Geobacillus stearothermophilus

abstract: The similarity of strains of thermophilic Geobacillus stearothermophilus (formerly Bacillus stearothermophilus), Anoxybacillus flavithermus (formerly Bacillus flavothermus), Bacillus licheniformis and Bacillus subtilis isolated from separate milk powder production runs from multiple factories was examined using a random amplified polymorphic DNA (RAPD) protocol. As a result of the analysis of the RAPD fingerprints and data relating to general growth and biochemical tests, over 98% of the 1470 isolates examined (grown at 55 °C) were assigned to the species G. stearothermophilus, A. flavithermus, B. licheniformis and B. subtilis. The G. stearothermophilus isolates were identified as being nearly identical to G. stearothermophilus (DSMZ 22; equivalent to ATCC 12980), or G. stearothermophilus var. calidolactis (DSMZ 1550). Three groups of isolates were found to be related to A. flavithermus (DSMZ 2641) by partial small ribosomal subunit (16S) sequence comparisons and shown to be interrelated by RAPD analyses with multiple primer sets. The thermophilic isolates of B. licheniformis were positively identified by comparison with type strains of B. licheniformis DSMZ 13 and DSMZ 8785. All of the B. subtilis strains shared bands in their RAPD profiles and were similar to a common B. subtilis type strain (DSMZ 10 and DSMZ 347). Overall, the most common and prevalent group of strains (group A) was demonstrated to be closely related to G. stearothermophilus (DSMZ 22).

text: A RAPD-based comparison of thermophilic bacilli from milk powders The similarity of strains of thermophilic Geobacillus stearothermophilus (formerly Bacillus stearothermophilus), Anoxybacillus flavithermus (formerly Bacillus flavothermus), Bacillus licheniformis and Bacillus subtilis isolated from separate milk powder production runs from multiple factories was examined using a random amplified polymorphic DNA (RAPD) protocol. As a result of the analysis of the RAPD fingerprints and data relating to general growth and biochemical tests, over 98% of the 1470 isolates examined (grown at 55 °C) were assigned to the species G. stearothermophilus, A. flavithermus, B. licheniformis and B. subtilis. The G. stearothermophilus isolates were identified as being nearly identical to G. stearothermophilus (DSMZ 22; equivalent to ATCC 12980), or G. stearothermophilus var. calidolactis (DSMZ 1550). Three groups of isolates were found to be related to A. flavithermus (DSMZ 2641) by partial small ribosomal subunit (16S) sequence comparisons and shown to be interrelated by RAPD analyses with multiple primer sets. The thermophilic isolates of B. licheniformis were positively identified by comparison with type strains of B. licheniformis DSMZ 13 and DSMZ 8785. All of the B. subtilis strains shared bands in their RAPD profiles and were similar to a common B. subtilis type strain (DSMZ 10 and DSMZ 347). Overall, the most common and prevalent group of strains (group A) was demonstrated to be closely related to G. stearothermophilus (DSMZ 22).

year: 2003

title: Evaluating the effects of team-skills training on subjective workload

authors: Prichard, Jane S.

subjects: subjective workload

abstract: This study evaluated the impact of a team-skills training intervention on students’ subjective experience of workload when working in collaborative groups. Three cohorts of students (N = 295) taking an undergraduate degree unit were compared across three successive years, in which presence or absence of training was varied. Students in trained groups reported lower levels of subjective workload than those in untrained groups and also performed better across a range of academic exercises. This effect was moderated by whether students were regrouped half-way through the academic year. Results are discussed in terms of theories of team-skill acquisition and issues in skill transferability caused by regrouping.

text: Evaluating the effects of team-skills training on subjective workload This study evaluated the impact of a team-skills training intervention on students’ subjective experience of workload when working in collaborative groups. Three cohorts of students (N = 295) taking an undergraduate degree unit were compared across three successive years, in which presence or absence of training was varied. Students in trained groups reported lower levels of subjective workload than those in untrained groups and also performed better across a range of academic exercises. This effect was moderated by whether students were regrouped half-way through the academic year. Results are discussed in terms of theories of team-skill acquisition and issues in skill transferability caused by regrouping.

year: 2010

title: Analysing A/O possession in Māori-language tweets

authors: Trye, David

subjects: A/O categories

abstract: This article contributes the first corpus-based study of possession in Māori, the indigenous language of Aotearoa New Zealand. Like most Polynesian languages, Māori has a dual possessive system involving a choice between the so-called A and O categories. While Māori grammars describe these categories in terms of the inherent semantic relationship between the possessum and possessor, there have been no large-scale corpus analyses demonstrating their use in natural contexts. Social media provide invaluable opportunities for such linguistic studies, capturing contemporary language use while alleviating the burden of gathering data through traditional means. We operationalise semantic distinctions to investigate possession in Māori-language tweets, focusing on the [possessum a/o possessor] construction (e.g., te tīmatanga o te wiki ‘the beginning of the week’). In our corpus comprising 2500 tweets produced by more than 200 individuals, we find that users leverage a wide array of noun types encompassing many different semantic relationships. We observe not only the expected predominance of the O category, but also a tendency for examples described by Māori grammars as A-marked to instead be O-marked (59%). Although the A category persists in the corpus, our findings suggest that language change could be underway. Our primary dataset can be explored interactively online.

text: Analysing A/O possession in Māori-language tweets This article contributes the first corpus-based study of possession in Māori, the indigenous language of Aotearoa New Zealand. Like most Polynesian languages, Māori has a dual possessive system involving a choice between the so-called A and O categories. While Māori grammars describe these categories in terms of the inherent semantic relationship between the possessum and possessor, there have been no large-scale corpus analyses demonstrating their use in natural contexts. Social media provide invaluable opportunities for such linguistic studies, capturing contemporary language use while alleviating the burden of gathering data through traditional means. We operationalise semantic distinctions to investigate possession in Māori-language tweets, focusing on the [possessum a/o possessor] construction (e.g., te tīmatanga o te wiki ‘the beginning of the week’). In our corpus comprising 2500 tweets produced by more than 200 individuals, we find that users leverage a wide array of noun types encompassing many different semantic relationships. We observe not only the expected predominance of the O category, but also a tendency for examples described by Māori grammars as A-marked to instead be O-marked (59%). Although the A category persists in the corpus, our findings suggest that language change could be underway. Our primary dataset can be explored interactively online.

year: 2024