title: Patrick Quinlan: a publican at Te Aroha and Auckland

authors: Hart, Philip

abstract: Born in Panmure in 1854 to an Irish Catholic military family, Patrick Quinlan became a publican in Te Aroha at the end of 1880. He invested in a small way in mines close to the township and in a much larger way in Waiorongomai mines. After giving up his hotel in early 1885 he was a carter and contractor, but struggled to make his way financially. He was a prominent participant in the social life of the community, notably in horse racing and in helping his church. Other family members lived in Te Aroha at the same time, notably his brother-in-law, Henry William Baskiville, a butcher.
After leaving Te Aroha in 1888, he became a very prominent and popular publican in Auckland, notable for the battles between his ‘free house’ and other hotels and brewers. Famous for his amiable nature, his involvement in horse racing and sport, and his charitable work, his impressive girth and ‘Irishness’ made him a cartoonist’s dream. During the 1890s he invested in the mining boom, and in 1899 stood for parliament as an independent Liberal, very unsuccessfully.
In 1908 he gave up his last hotel to settle on a farm at Taihoa, near Morrinsville. Once again he became a prominent member of the community, especially through his involvement with the Matamata Racing Club. When he died he was fondly remembered as having been ‘generous and open-handed to a fault’.

text: Patrick Quinlan: a publican at Te Aroha and Auckland Born in Panmure in 1854 to an Irish Catholic military family, Patrick Quinlan became a publican in Te Aroha at the end of 1880. He invested in a small way in mines close to the township and in a much larger way in Waiorongomai mines. After giving up his hotel in early 1885 he was a carter and contractor, but struggled to make his way financially. He was a prominent participant in the social life of the community, notably in horse racing and in helping his church. Other family members lived in Te Aroha at the same time, notably his brother-in-law, Henry William Baskiville, a butcher.
After leaving Te Aroha in 1888, he became a very prominent and popular publican in Auckland, notable for the battles between his ‘free house’ and other hotels and brewers. Famous for his amiable nature, his involvement in horse racing and sport, and his charitable work, his impressive girth and ‘Irishness’ made him a cartoonist’s dream. During the 1890s he invested in the mining boom, and in 1899 stood for parliament as an independent Liberal, very unsuccessfully.
In 1908 he gave up his last hotel to settle on a farm at Taihoa, near Morrinsville. Once again he became a prominent member of the community, especially through his involvement with the Matamata Racing Club. When he died he was fondly remembered as having been ‘generous and open-handed to a fault’.

year: 2016

title: Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry

authors: McKenzie, Joanna Leigh

subjects: Mycobacteria

abstract: The VapBC toxin-antitoxin (TA) family is the largest of nine identified TA families. The toxin, VapC, is a metal-dependent ribonuclease that is inhibited by its cognate antitoxin, VapB. Although the VapBCs are the largest TA family, little is known about their biological roles. Here we describe a new general method for the overexpression and purification of toxic VapC proteins and subsequent determination of their RNase sequence-specificity. Functional VapC was isolated by expression of the nontoxic VapBC complex, followed by removal of the labile antitoxin (VapB) using limited trypsin digestion. We have then developed a sensitive and robust method for determining VapC ribonuclease sequence-specificity. This technique employs the use of Pentaprobes as substrates for VapC. These are RNA sequences encoding every combination of five bases. We combine the RNase reaction with MALDI-TOF MS to detect and analyze the cleavage products and thus determine the RNA cut sites. Successful MALDI-TOF MS analysis of RNA fragments is acutely dependent on sample preparation methods. The sequencespecificity of four VapC proteins from two different organisms (VapCPAE0151 and VapCPAE2754 from Pyrobaculum aerophilum, and VapCRv0065 and VapCRv0617 from Mycobacterium tuberculosis) was successfully determined using the described strategy. This rapid and sensitive method can be applied to determine the sequence-specificity of VapC ribonucleases along with other RNA interferases (such as MazF) from a range of organisms. Published by Cold Spring Harbor Laboratory Press.

text: Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry The VapBC toxin-antitoxin (TA) family is the largest of nine identified TA families. The toxin, VapC, is a metal-dependent ribonuclease that is inhibited by its cognate antitoxin, VapB. Although the VapBCs are the largest TA family, little is known about their biological roles. Here we describe a new general method for the overexpression and purification of toxic VapC proteins and subsequent determination of their RNase sequence-specificity. Functional VapC was isolated by expression of the nontoxic VapBC complex, followed by removal of the labile antitoxin (VapB) using limited trypsin digestion. We have then developed a sensitive and robust method for determining VapC ribonuclease sequence-specificity. This technique employs the use of Pentaprobes as substrates for VapC. These are RNA sequences encoding every combination of five bases. We combine the RNase reaction with MALDI-TOF MS to detect and analyze the cleavage products and thus determine the RNA cut sites. Successful MALDI-TOF MS analysis of RNA fragments is acutely dependent on sample preparation methods. The sequencespecificity of four VapC proteins from two different organisms (VapCPAE0151 and VapCPAE2754 from Pyrobaculum aerophilum, and VapCRv0065 and VapCRv0617 from Mycobacterium tuberculosis) was successfully determined using the described strategy. This rapid and sensitive method can be applied to determine the sequence-specificity of VapC ribonucleases along with other RNA interferases (such as MazF) from a range of organisms. Published by Cold Spring Harbor Laboratory Press.

year: 2012

title: Tariao Whitiwhitiora

authors: Totorewa, Te Oko Horoi

abstract: I te tau 1875 i tiihooka tahi atu a Kiingi Taawhiao ki Taranaki ka tau ki mua ki te aroaro o Te Uahaumeene. Matua raa ko te rongomau me te rangimaaria, te kotahitanga me te aroha o teetehi ki teetehi maa roto mai i te whakapono ki te atua te whaainga i haere ai ia.

Ka tutuki i a Kiingi Taawhiao te rongomau me te rangimaaria, te kotahitanga me te aroha o teetehi ki teetehi maa te whakakotahi i ngaa ngaa koorero, ngaa hiitori, ngaa whakapapa, ngaa maataapono mai o te taioretanga o ngoo taatou tuupuna ka toopuuria ki te whakapono Tariao.

He whakapono teenei i whakaorangia e ia, e Kiingi Taawhiao maa roto mai i te tongikura, “E Ua, e toru ngaa taonga kei te kapu o taku ringa, ko te Mihinare, ko te Katorika me te Weteriana. Homai taau ka whaa. Kopi katoa ngeenei taonga i au ki te kapu o taku ringa, ka puta ko te Paimaarire.” Ko te whakapono Tariao me ngaa karakia Paimaarie ka hikitia, ka haapaingia tonu e maatou ngaa mangainga, ngaa kaiwhakapuumau i te Kiingitanga inaa raa koia teenei te whakapono matua moo te Kiingitanga.

I teenei tuhinga whakapae ko te whakapono Tariao te taahuhu o ngaa koorero. I konei ngahere mai ai te kaupapa matua o teenei tuhinga.

“He kura Tariao he kura whakapapa, te herenga ki te whakaoranga o Waikato tangata”

Ko teenei kaupapa ka whakarukuhia maa te rangahau i te ahunga mai o te maatauranga kei aa teenei whakapono i whea. Ka rangahautia ko te whakapaparanga o Kiingi Taawhiao mai i te Kore, ki a Rangi raaua ko Papa heke iho mai ki a Hoturoa, aa, ka tau ki a ia ki a Kiingi Taawhiao inaa raa, ko ngeenei momo he mea e tauiratia ana ki roto ki ngaa karakia, ki ngaa Kura kei teenei whakapono.

Ka hereherea ko Te Uahaumeene ki koneki naa, ki ngaa koorero e kitea ai maa whea i puaawai mai ai te Tariao me ngaa karakia Paimaarire. Ko te Kiingitanga me ngaana pehonga kaupapa e taapae atu ana ki te oranga o teenei whakapono maa te whakamahi i teenei whakapono teeraa ka waahia. Taea noatia ki te puutakenga mai o te whakapono ake, ngoonaa koorero, ngoona tuuranga, ngoona karakia, ngoona kura, ngoona whakamaaramatanga, tau iho mai ki te reo
 
tupuna o roto. Ko ngaa aupiki me ngaa auheke, te whakaraakeiora hoki i waatea mai, i whaipaanga atu ki te whakapono Tariao ka whakamaaramahia. Ka whakamutua atu me te taatari i ngaa putanga ka puta e kite ake ai me he herenga too teenei whakapono ki te whakaoranga o Waikato tangata.

text: Tariao Whitiwhitiora I te tau 1875 i tiihooka tahi atu a Kiingi Taawhiao ki Taranaki ka tau ki mua ki te aroaro o Te Uahaumeene. Matua raa ko te rongomau me te rangimaaria, te kotahitanga me te aroha o teetehi ki teetehi maa roto mai i te whakapono ki te atua te whaainga i haere ai ia.

Ka tutuki i a Kiingi Taawhiao te rongomau me te rangimaaria, te kotahitanga me te aroha o teetehi ki teetehi maa te whakakotahi i ngaa ngaa koorero, ngaa hiitori, ngaa whakapapa, ngaa maataapono mai o te taioretanga o ngoo taatou tuupuna ka toopuuria ki te whakapono Tariao.

He whakapono teenei i whakaorangia e ia, e Kiingi Taawhiao maa roto mai i te tongikura, “E Ua, e toru ngaa taonga kei te kapu o taku ringa, ko te Mihinare, ko te Katorika me te Weteriana. Homai taau ka whaa. Kopi katoa ngeenei taonga i au ki te kapu o taku ringa, ka puta ko te Paimaarire.” Ko te whakapono Tariao me ngaa karakia Paimaarie ka hikitia, ka haapaingia tonu e maatou ngaa mangainga, ngaa kaiwhakapuumau i te Kiingitanga inaa raa koia teenei te whakapono matua moo te Kiingitanga.

I teenei tuhinga whakapae ko te whakapono Tariao te taahuhu o ngaa koorero. I konei ngahere mai ai te kaupapa matua o teenei tuhinga.

“He kura Tariao he kura whakapapa, te herenga ki te whakaoranga o Waikato tangata”

Ko teenei kaupapa ka whakarukuhia maa te rangahau i te ahunga mai o te maatauranga kei aa teenei whakapono i whea. Ka rangahautia ko te whakapaparanga o Kiingi Taawhiao mai i te Kore, ki a Rangi raaua ko Papa heke iho mai ki a Hoturoa, aa, ka tau ki a ia ki a Kiingi Taawhiao inaa raa, ko ngeenei momo he mea e tauiratia ana ki roto ki ngaa karakia, ki ngaa Kura kei teenei whakapono.

Ka hereherea ko Te Uahaumeene ki koneki naa, ki ngaa koorero e kitea ai maa whea i puaawai mai ai te Tariao me ngaa karakia Paimaarire. Ko te Kiingitanga me ngaana pehonga kaupapa e taapae atu ana ki te oranga o teenei whakapono maa te whakamahi i teenei whakapono teeraa ka waahia. Taea noatia ki te puutakenga mai o te whakapono ake, ngoonaa koorero, ngoona tuuranga, ngoona karakia, ngoona kura, ngoona whakamaaramatanga, tau iho mai ki te reo
 
tupuna o roto. Ko ngaa aupiki me ngaa auheke, te whakaraakeiora hoki i waatea mai, i whaipaanga atu ki te whakapono Tariao ka whakamaaramahia. Ka whakamutua atu me te taatari i ngaa putanga ka puta e kite ake ai me he herenga too teenei whakapono ki te whakaoranga o Waikato tangata.

year: 2022