title: A Geografia Mais Íntima: O Corpo

authors: Johnston, Lynda

abstract: El cuerpo como lugar, se centra en la geografía del cuerpo como un lugar colonizado, moldeado por el poder pero, que a su vez, ofrece resistencia. Es un espacio socialmente construido donde se toma al cuer-po como lugar, como primer territorio a ser defendido, sobre todo en el contexto brasileño, según Joseli Maria Silva, Marcio Jose Ornat y Alides Baptista Chimin Junior, en el cual se analizan las Expedições sobre corpos na geografia brasileira: trilhas equivocadas e rumos encontrados. También, seguido por el entramado de relaciones de poder que operan en diferentes escalas, ello es planteado por los autores Lynda Johnston y Robyn Longhurst, en su texto A Geografia mais íntima: o corpo.

El cuerpo como lugar, focuses on the geography of the body as a colonised place, shaped by power, but which in turn offers resistance. It is a socially constructed space where the body is taken as a place, as the first territory to be defended, especially in the Brazilian context, according to Joseli Maria Silva, Marcio Jose Ornat and Alides Baptista Chimin Junior, in which the Expeditions on bodies in Brazilian geography are analysed: mistaken trails and directions found. Also, followed by the entanglement of power relations that operate at different scales, this is put forward by the authors Lynda Johnston and Robyn Longhurst, in their text The most intimate geography: the body - Translated with DeepL.

text: A Geografia Mais Íntima: O Corpo El cuerpo como lugar, se centra en la geografía del cuerpo como un lugar colonizado, moldeado por el poder pero, que a su vez, ofrece resistencia. Es un espacio socialmente construido donde se toma al cuer-po como lugar, como primer territorio a ser defendido, sobre todo en el contexto brasileño, según Joseli Maria Silva, Marcio Jose Ornat y Alides Baptista Chimin Junior, en el cual se analizan las Expedições sobre corpos na geografia brasileira: trilhas equivocadas e rumos encontrados. También, seguido por el entramado de relaciones de poder que operan en diferentes escalas, ello es planteado por los autores Lynda Johnston y Robyn Longhurst, en su texto A Geografia mais íntima: o corpo.

El cuerpo como lugar, focuses on the geography of the body as a colonised place, shaped by power, but which in turn offers resistance. It is a socially constructed space where the body is taken as a place, as the first territory to be defended, especially in the Brazilian context, according to Joseli Maria Silva, Marcio Jose Ornat and Alides Baptista Chimin Junior, in which the Expeditions on bodies in Brazilian geography are analysed: mistaken trails and directions found. Also, followed by the entanglement of power relations that operate at different scales, this is put forward by the authors Lynda Johnston and Robyn Longhurst, in their text The most intimate geography: the body - Translated with DeepL.

year: 2023

title: Properties and stabilization of an extracellular α-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN 1: enzyme activity at 130°C

authors: Piller, Karin

subjects: α-glucosidase

abstract: An extracellular α-glucosidase from the thermophilic archaebacterium Thermococcus strain AN1 was purified 875-fold in five steps (Hiload Q-Sepharose, phenyl Sepharose, HPHT-hydroxyapatite, gel filtration and Mono Q chromatography) with a yield of 4%. It is a monomer with a molecular mass of about 60 kDa and a pI around 5. At 98°C, the purified enzyme in buffer has a half-life around 35 min, which is increased to around 215 min in presence of l% (w/v) dithiothreitol and 1% (w/v) BSA. Dithiothreitol (1%, w/v) and BSA (0.4%, w/v) also substantially increase the enzyme activity. The Km at 75°C is 0.41 mM with pNP-α-  -glucopyranoside as substrate. The substrate preference of the enzyme is: pNP-α-D-glucoside > nigerose > panose > palatinose > isomaltose > maltose and turanose. No activity was found against starch, pullulan, amylose, maltotriose, maltotetraose, isomaltotriose, cellobiose and β-gentiobiose. A variety of techniques including immobilization (e.g., on epoxy and glass beads), chemical modification (cross- and cocross-linking) and the use of additives (including polyhydroxylic molecules, BSA, salts, etc.) were applied to enhance stability at temperatures above 100°C. The half-life could be increased from about 4 min at 110°C to 30–60 min at 130°C in presence of 90% (w/v) sorbitol, 1% (w/v) dithiothreitol and l% (w/v) BSA, and by cocross-linking with BSA in the presence of 90% (w/v) sorbitol. The stabilized enzyme showed good activity at 130°C.

text: Properties and stabilization of an extracellular α-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN 1: enzyme activity at 130°C An extracellular α-glucosidase from the thermophilic archaebacterium Thermococcus strain AN1 was purified 875-fold in five steps (Hiload Q-Sepharose, phenyl Sepharose, HPHT-hydroxyapatite, gel filtration and Mono Q chromatography) with a yield of 4%. It is a monomer with a molecular mass of about 60 kDa and a pI around 5. At 98°C, the purified enzyme in buffer has a half-life around 35 min, which is increased to around 215 min in presence of l% (w/v) dithiothreitol and 1% (w/v) BSA. Dithiothreitol (1%, w/v) and BSA (0.4%, w/v) also substantially increase the enzyme activity. The Km at 75°C is 0.41 mM with pNP-α-  -glucopyranoside as substrate. The substrate preference of the enzyme is: pNP-α-D-glucoside > nigerose > panose > palatinose > isomaltose > maltose and turanose. No activity was found against starch, pullulan, amylose, maltotriose, maltotetraose, isomaltotriose, cellobiose and β-gentiobiose. A variety of techniques including immobilization (e.g., on epoxy and glass beads), chemical modification (cross- and cocross-linking) and the use of additives (including polyhydroxylic molecules, BSA, salts, etc.) were applied to enhance stability at temperatures above 100°C. The half-life could be increased from about 4 min at 110°C to 30–60 min at 130°C in presence of 90% (w/v) sorbitol, 1% (w/v) dithiothreitol and l% (w/v) BSA, and by cocross-linking with BSA in the presence of 90% (w/v) sorbitol. The stabilized enzyme showed good activity at 130°C.

year: 1996

title: Stigmatising gang narratives, housing, and the social policing of Māori women

authors: Lewis, Cassandra Louise Terauhina

subjects: Gang narratives

abstract: It is well established that negative stigmas are used to dehumanise and to limit, restrict, and deny assistance to poor and racially marginalised populations, especially single mothers. While scholars have examined racial social control with regard to gangs, how the stigma of gang affiliation influences access to quality housing has been scantly examined in New Zealand. Employing discourse analysis to examine parliamentary speeches regarding the Organised Crime Bill and Residential Tenancy Amendment Bill (2009–2018), this project explores how gangs are framed in housing-related political discourse. Findings reveal that language used by policymakers constructs gangs as being associated largely with Māori through three main tactics: referencing ‘ethnic’ gangs as the only entity/focus of organised crime; omitting Pākehā/White gangs and white-collar crime; and identifying areas/towns with high Māori populations as in need of targeted social control. Such framing strategies fashioned a criminalising narrative around Māori as gang members and as inherently violent that permeates housing-related political rhetoric used to frame ‘bad’ or ‘undesirable’ tenants enabling policymakers to stigmatise Māori as the ‘criminal other’. This motif carries severe implications with regard to life chances of Māori women, especially as it relates to housing screening practices and the ability to secure social housing.

text: Stigmatising gang narratives, housing, and the social policing of Māori women It is well established that negative stigmas are used to dehumanise and to limit, restrict, and deny assistance to poor and racially marginalised populations, especially single mothers. While scholars have examined racial social control with regard to gangs, how the stigma of gang affiliation influences access to quality housing has been scantly examined in New Zealand. Employing discourse analysis to examine parliamentary speeches regarding the Organised Crime Bill and Residential Tenancy Amendment Bill (2009–2018), this project explores how gangs are framed in housing-related political discourse. Findings reveal that language used by policymakers constructs gangs as being associated largely with Māori through three main tactics: referencing ‘ethnic’ gangs as the only entity/focus of organised crime; omitting Pākehā/White gangs and white-collar crime; and identifying areas/towns with high Māori populations as in need of targeted social control. Such framing strategies fashioned a criminalising narrative around Māori as gang members and as inherently violent that permeates housing-related political rhetoric used to frame ‘bad’ or ‘undesirable’ tenants enabling policymakers to stigmatise Māori as the ‘criminal other’. This motif carries severe implications with regard to life chances of Māori women, especially as it relates to housing screening practices and the ability to secure social housing.

year: 2020