title: Assessing risky online behavior for cyberharassment and cyberstalking among university students in New Zealand

authors: Tompson, Lisa

subjects: fear

abstract: This study examines the relationship between two types of cyberabuse-cyberharassment and cyberstalking-and constructs from the lifestyle-routine activity approach relating to the visibility and accessibility of victims (targets) online. Data were collected from 185 university students in New Zealand via an online survey. Descriptive statistics shows that cyberabuse victimization experiences in the previous year were common, but respondents only reported associated feelings of fear or distress for half of these experiences. Findings indicated that using dating apps is associated with an increased risk of cyberharassment, and including details of other social network accounts in a user profile is associated with an increased risk of cyberstalking. Interestingly, demographic characteristics did not correlate with victimization. Overall, this research underscores the importance of differentiating between cyberharassment and cyberstalking to inform prevention activities.

text: Assessing risky online behavior for cyberharassment and cyberstalking among university students in New Zealand This study examines the relationship between two types of cyberabuse-cyberharassment and cyberstalking-and constructs from the lifestyle-routine activity approach relating to the visibility and accessibility of victims (targets) online. Data were collected from 185 university students in New Zealand via an online survey. Descriptive statistics shows that cyberabuse victimization experiences in the previous year were common, but respondents only reported associated feelings of fear or distress for half of these experiences. Findings indicated that using dating apps is associated with an increased risk of cyberharassment, and including details of other social network accounts in a user profile is associated with an increased risk of cyberstalking. Interestingly, demographic characteristics did not correlate with victimization. Overall, this research underscores the importance of differentiating between cyberharassment and cyberstalking to inform prevention activities.

year: 2025

title: Elucidating the molecular basis of dihydroxyacetone (DHA) production in Leptospermum nectar

authors: Grierson, Ella

subjects: dihydroxyacetone

abstract: Mānuka (Leptospermum scoparium) nectar contains variable amounts of dihydroxyacetone (DHA), a triose sugar that is the precursor to methylglyoxal (MGO), the antimicrobial compound underpinning the high value of mānuka honey and forming the basis of the Aotearoa-New Zealand honey industry. This trait is unique to some species within the Myrtaceae. The molecular basis of this high-value trait is unknown and identifying it would allow development of gene-based tools to identify high value germplasm to inform replanting and breeding programmes. Despite the fundamental importance of nectar to crop pollination and honey industries, the genetic control of nectary function is poorly understood, especially in non-model species. This thesis aims to elucidate the molecular basis of DHA production in Leptospermum nectar, through identifying associated genes and genomic regions, followed by further exploration of gene regulation and enzyme characterisation in vitro and in vivo. 
RNAseq analysis identified nectary-associated genes differentially expressed between high and low nectar-DHA genotypes of L. scoparium, and a mānuka high-density linkage map and quantitative trait loci (QTL) mapping population revealed genetic regions associated with nectar DHA content. Expression and QTL analyses both pointed to the involvement of a phosphatase gene, LsSgpp2, as its expression correlated with nectar DHA accumulation and it co-located with a QTL on chromosome 4. To investigate Sgpp2 and its complex locus further, we produced high-quality genomes and hypanthium transcriptomes for two further Leptospermeae species with contrasting DHA phenotypes – Leptospermum morrisonii and Gaudium laevigatum. Expression patterns of Sgpp2 again correlated with nectar DHA in these species, and comparative analyses of Sgpp sequences within the Myrtaceae indicated that high Sgpp2 expression was likely ancestral in DHA producing taxa, followed by repeated loss of the trait. Comparison within and between Sgpp genes with differing expression profiles identified regions unique to the highly expressed Sgpp2 genes, which contained two C-box motifs, and a bZIP11 transcription factor predicted to bind to these motifs was identified as significantly differentially expressed in the RNAseq dataset. The bZIP11 gene was subsequently found to co-locate with another of the QTLs identified, further supporting its involvement. The L. morrisonii promoter drove strong nectary-specific expression in transgenic lines of Petunia and Nicotiana, showing elements essential for Sgpp2 expression are within that region. However, the promoter in 13 DHA producing L. scoparium genotypes was found to be similar, indicating variation in nectar DHA amounts may be conferred by further complex transcriptional regulation fine tuning Sgpp2 expression from beyond the promoter region analysed here. Functional analyses demonstrated that LmSGPP2 can dephosphorylate dihydroxyacetone-phosphate (DHAP) to produce DHA and phosphate in vitro. Transgenic Petunia and Nicotiana lines were created to characterise LmSGPP2 activity in vivo, but results were inconclusive due to low and mis-located expression of the transgene. 
Together our results suggest Sgpp2 may contribute to maintaining phosphate homeostasis in a photosynthesising nectary, potentially evolving due to low phosphate availability – which is common in Australia where this tribe originates. This work advances our understanding of nectar biology and reports some of the first QTLs and genes linked to a low abundance nectar compound. The genes and genomic regions identified here provide a foundation for developing tools to identify high-value germplasm – ensuring biodiversity can be maintained while increasing high-value honey production.

text: Elucidating the molecular basis of dihydroxyacetone (DHA) production in Leptospermum nectar Mānuka (Leptospermum scoparium) nectar contains variable amounts of dihydroxyacetone (DHA), a triose sugar that is the precursor to methylglyoxal (MGO), the antimicrobial compound underpinning the high value of mānuka honey and forming the basis of the Aotearoa-New Zealand honey industry. This trait is unique to some species within the Myrtaceae. The molecular basis of this high-value trait is unknown and identifying it would allow development of gene-based tools to identify high value germplasm to inform replanting and breeding programmes. Despite the fundamental importance of nectar to crop pollination and honey industries, the genetic control of nectary function is poorly understood, especially in non-model species. This thesis aims to elucidate the molecular basis of DHA production in Leptospermum nectar, through identifying associated genes and genomic regions, followed by further exploration of gene regulation and enzyme characterisation in vitro and in vivo. 
RNAseq analysis identified nectary-associated genes differentially expressed between high and low nectar-DHA genotypes of L. scoparium, and a mānuka high-density linkage map and quantitative trait loci (QTL) mapping population revealed genetic regions associated with nectar DHA content. Expression and QTL analyses both pointed to the involvement of a phosphatase gene, LsSgpp2, as its expression correlated with nectar DHA accumulation and it co-located with a QTL on chromosome 4. To investigate Sgpp2 and its complex locus further, we produced high-quality genomes and hypanthium transcriptomes for two further Leptospermeae species with contrasting DHA phenotypes – Leptospermum morrisonii and Gaudium laevigatum. Expression patterns of Sgpp2 again correlated with nectar DHA in these species, and comparative analyses of Sgpp sequences within the Myrtaceae indicated that high Sgpp2 expression was likely ancestral in DHA producing taxa, followed by repeated loss of the trait. Comparison within and between Sgpp genes with differing expression profiles identified regions unique to the highly expressed Sgpp2 genes, which contained two C-box motifs, and a bZIP11 transcription factor predicted to bind to these motifs was identified as significantly differentially expressed in the RNAseq dataset. The bZIP11 gene was subsequently found to co-locate with another of the QTLs identified, further supporting its involvement. The L. morrisonii promoter drove strong nectary-specific expression in transgenic lines of Petunia and Nicotiana, showing elements essential for Sgpp2 expression are within that region. However, the promoter in 13 DHA producing L. scoparium genotypes was found to be similar, indicating variation in nectar DHA amounts may be conferred by further complex transcriptional regulation fine tuning Sgpp2 expression from beyond the promoter region analysed here. Functional analyses demonstrated that LmSGPP2 can dephosphorylate dihydroxyacetone-phosphate (DHAP) to produce DHA and phosphate in vitro. Transgenic Petunia and Nicotiana lines were created to characterise LmSGPP2 activity in vivo, but results were inconclusive due to low and mis-located expression of the transgene. 
Together our results suggest Sgpp2 may contribute to maintaining phosphate homeostasis in a photosynthesising nectary, potentially evolving due to low phosphate availability – which is common in Australia where this tribe originates. This work advances our understanding of nectar biology and reports some of the first QTLs and genes linked to a low abundance nectar compound. The genes and genomic regions identified here provide a foundation for developing tools to identify high-value germplasm – ensuring biodiversity can be maintained while increasing high-value honey production.

year: 2025

title: Te Reo Tātai: The Development of a Mathematics Register for Māori-Medium Schooling

authors: Trinick, Anthony

subjects: Indigenous Mathematics education

abstract: This thesis investigates the development of a lexicon and register to facilitate the teaching of mathematics in the medium of te reo Māori. This sociolinguistic development is situated within the wider Māori language revitalisation movement in Aotearoa/New Zealand, of which Māori-medium education is a central component. 
Lexication of a language is a subdomain of corpus planning. Corpus, status and language-in-education planning are considered the three major interconnected domains of language planning and policy (LPP). For decades, the primary focus of LPP research has been on the activities of states and their agencies at the macro (national) level. The significance of this study is that it addresses this gap in the research, by investigating the development of LPP at the meso/micro-level—in this case, with respect to the development of a mathematics register for an endangered indigenous language, te reo Māori.
The thesis draws on two major participant data sources. The first is the narratives of key individuals at the forefront of lexical development for Māori-medium mathematics who recounted their experiences. The second is interviews with principals and teachers from two Māori-medium case study schools to examine their own learning and usage of the standardised pāngarau lexicon.
Two complementary theoretical frameworks were used to investigate the topic. First, an LPP theoretical framework was applied to investigate the complex relationships between the various levels of LPP that have impacted on the evolution of te reo Māori and Māori-medium schooling. Second, a linguistic framework, systemic functional linguistics (SFL), was used to investigate the linguistic and pedagogical issues.
Thesis findings show that the status of te reo Māori was variously affected by linguistic ideologies such as assimilation, which in turn led to such policies as English only in schooling. As a consequence, at the micro-level of LPP, Māori-medium mathematics development went into a hiatus for over 100 years, and at the macro-level, considerable language shift occurred to English, causing te reo Maori to become endangered.
In response to language revitalisation efforts, the (re) vernacularisation of te reo Māori over the past 30 years has exhibited a complex combination of both bottom-up and top-down LPP. Within this, early pāngarau lexical developments were largely driven by local schools and their communities, which sometimes centred on the maintenance of their tribal dialects, often in conflict with later LPP agency language goals. 
Subsequently, lexication for pāngarau was influenced by macro-level sociolinguistic beliefs such as linguistic purism, which in turn have influenced the technical approach to creating terms and may have created particular pedagogical issues, such as a lack of intertranslatability between te reo Māori and English at the higher levels of mathematics study. 
While Māori-medium schooling has contributed significantly to language revitalisation in the past 30 years, its development has been very ad hoc. In the absence of a “formal” language plan for Māori-medium education, policy has been significantly driven by national education (English-medium) imperatives since the late 1970s to the present day. On the other hand, these policies have favoured mathematics education and directly supported the development of the Māori-medium specialised lexicon and register.

text: Te Reo Tātai: The Development of a Mathematics Register for Māori-Medium Schooling This thesis investigates the development of a lexicon and register to facilitate the teaching of mathematics in the medium of te reo Māori. This sociolinguistic development is situated within the wider Māori language revitalisation movement in Aotearoa/New Zealand, of which Māori-medium education is a central component. 
Lexication of a language is a subdomain of corpus planning. Corpus, status and language-in-education planning are considered the three major interconnected domains of language planning and policy (LPP). For decades, the primary focus of LPP research has been on the activities of states and their agencies at the macro (national) level. The significance of this study is that it addresses this gap in the research, by investigating the development of LPP at the meso/micro-level—in this case, with respect to the development of a mathematics register for an endangered indigenous language, te reo Māori.
The thesis draws on two major participant data sources. The first is the narratives of key individuals at the forefront of lexical development for Māori-medium mathematics who recounted their experiences. The second is interviews with principals and teachers from two Māori-medium case study schools to examine their own learning and usage of the standardised pāngarau lexicon.
Two complementary theoretical frameworks were used to investigate the topic. First, an LPP theoretical framework was applied to investigate the complex relationships between the various levels of LPP that have impacted on the evolution of te reo Māori and Māori-medium schooling. Second, a linguistic framework, systemic functional linguistics (SFL), was used to investigate the linguistic and pedagogical issues.
Thesis findings show that the status of te reo Māori was variously affected by linguistic ideologies such as assimilation, which in turn led to such policies as English only in schooling. As a consequence, at the micro-level of LPP, Māori-medium mathematics development went into a hiatus for over 100 years, and at the macro-level, considerable language shift occurred to English, causing te reo Maori to become endangered.
In response to language revitalisation efforts, the (re) vernacularisation of te reo Māori over the past 30 years has exhibited a complex combination of both bottom-up and top-down LPP. Within this, early pāngarau lexical developments were largely driven by local schools and their communities, which sometimes centred on the maintenance of their tribal dialects, often in conflict with later LPP agency language goals. 
Subsequently, lexication for pāngarau was influenced by macro-level sociolinguistic beliefs such as linguistic purism, which in turn have influenced the technical approach to creating terms and may have created particular pedagogical issues, such as a lack of intertranslatability between te reo Māori and English at the higher levels of mathematics study. 
While Māori-medium schooling has contributed significantly to language revitalisation in the past 30 years, its development has been very ad hoc. In the absence of a “formal” language plan for Māori-medium education, policy has been significantly driven by national education (English-medium) imperatives since the late 1970s to the present day. On the other hand, these policies have favoured mathematics education and directly supported the development of the Māori-medium specialised lexicon and register.

year: 2015