title: Effects on adhesion molecule expression and lymphocytes in the bovine mammary gland following intra-mammary immunisation

authors: Hodgkinson, A.J.

subjects: bovine

abstract: Changes to adhesion molecule expression and lymphocyte populations were evaluated in alveolar mammary tissue collected from cows following an immunisation protocol that involved intra-mammary inoculation to induce an IgA response in mammary secretions. The right quarters of the udder were immunised; the left side acted as a control. Antibody titres in secretions showed that at least two animals responded with antigen-specific IgA. Numbers of T-lymphocytes were 4-fold higher in immunised glands compared with controls (P < 0.05). IgA-, IgM- and IgG-positive cell numbers were significantly higher (P < 0.01) in immunised glands compared with controls in three of the four cows. No mucosal addressin molecule-1 (MAdCAM-1), vascular cell-adhesion molecule-1 (VCAM-1) or peripheral node addressin (PNAd) protein expression was detected on smaller venules that stained positively for von Willebrand factor in alveolar mammary tissues, from either immunised or control glands. Both VCAM-1 and PNAd were detected on smaller venules in supramammary lymph nodes, however, there was no significant difference between immunised and control glands. Quantification of MAdCAM-1 mRNA showed very low expression in both immunised and control alveolar tissue compared with Peyer's patch positive-control tissue. These findings suggest that the bovine mammary gland is capable of a mucosal antibody response; however, MAdCAM-1 is not involved with lymphocyte homing to the mammary gland in this species.

text: Effects on adhesion molecule expression and lymphocytes in the bovine mammary gland following intra-mammary immunisation Changes to adhesion molecule expression and lymphocyte populations were evaluated in alveolar mammary tissue collected from cows following an immunisation protocol that involved intra-mammary inoculation to induce an IgA response in mammary secretions. The right quarters of the udder were immunised; the left side acted as a control. Antibody titres in secretions showed that at least two animals responded with antigen-specific IgA. Numbers of T-lymphocytes were 4-fold higher in immunised glands compared with controls (P < 0.05). IgA-, IgM- and IgG-positive cell numbers were significantly higher (P < 0.01) in immunised glands compared with controls in three of the four cows. No mucosal addressin molecule-1 (MAdCAM-1), vascular cell-adhesion molecule-1 (VCAM-1) or peripheral node addressin (PNAd) protein expression was detected on smaller venules that stained positively for von Willebrand factor in alveolar mammary tissues, from either immunised or control glands. Both VCAM-1 and PNAd were detected on smaller venules in supramammary lymph nodes, however, there was no significant difference between immunised and control glands. Quantification of MAdCAM-1 mRNA showed very low expression in both immunised and control alveolar tissue compared with Peyer's patch positive-control tissue. These findings suggest that the bovine mammary gland is capable of a mucosal antibody response; however, MAdCAM-1 is not involved with lymphocyte homing to the mammary gland in this species.

year: 2009

title: Risky business: Developmental neuroscience and the culpability of young killers

authors: Midson, Brenda

subjects: adolescence

abstract: Children and young people who kill do not always act with the mental acuity expected of someone who has reached full maturity, yet when they are charged with murder or manslaughter they are often tried as adults. This contradicts the basic principle of criminal responsibility that criminalisation is based upon moral blameworthiness. A conviction for murder requires at least a conscious appreciation of a real risk of death. Recent research by developmental neuroscientists shows that adolescence is a developmental stage and that the adolescent brain is not capable of the same level of reasoning expected in the average adult. This article suggests that the criminal justice system can adapt to these recent advances in knowledge via specific defences of a diminished capacity class, or by allowing evidence of general adolescent brain development to support defence arguments that a young accused did not form the mens rea required for murder.

text: Risky business: Developmental neuroscience and the culpability of young killers Children and young people who kill do not always act with the mental acuity expected of someone who has reached full maturity, yet when they are charged with murder or manslaughter they are often tried as adults. This contradicts the basic principle of criminal responsibility that criminalisation is based upon moral blameworthiness. A conviction for murder requires at least a conscious appreciation of a real risk of death. Recent research by developmental neuroscientists shows that adolescence is a developmental stage and that the adolescent brain is not capable of the same level of reasoning expected in the average adult. This article suggests that the criminal justice system can adapt to these recent advances in knowledge via specific defences of a diminished capacity class, or by allowing evidence of general adolescent brain development to support defence arguments that a young accused did not form the mens rea required for murder.

year: 2012

title: Three Pacific writers from Aotearoa discuss Indigenous languages and visual poetry

authors: Salsano, Marama

abstract: This article draws on several months of conversations, creative workshopping, and writing sessions between Māori writers Ammon Hāwea Apiata and Marama Salsano, and Rotuman writer Mere Taito. Here, we contemplate the presence of Māori and Rotuman languages in our visual poetry. For too long, critical work about English language writing by Indigenous writers from the Pacific has been Eurocentric. Papua New Guinean writer-scholar Steven Winduo suggests the need to unwrite this “imagined Oceania,” while Māori author Keri Hulme writes disparagingly of the lower-cased ‘gods of literature’, and in her ReadNZ lecture, Sāmoan-Māori fantasy writer Lani Wendt Young describes traditional publishing as the “white castle of literature.” While white castled gods of literature have historically ignored the everyday vibrancy of Pacific voices, Indigenous writers from the Pacific continue to unapologetically write, read, experiment, critique, and play with words. Into this complexity, we acknowledge that for many Indigenous writers from the Pacific, English language Eurocentric thought and texts dominate our lives; many of us are second language learners of our languages.

text: Three Pacific writers from Aotearoa discuss Indigenous languages and visual poetry This article draws on several months of conversations, creative workshopping, and writing sessions between Māori writers Ammon Hāwea Apiata and Marama Salsano, and Rotuman writer Mere Taito. Here, we contemplate the presence of Māori and Rotuman languages in our visual poetry. For too long, critical work about English language writing by Indigenous writers from the Pacific has been Eurocentric. Papua New Guinean writer-scholar Steven Winduo suggests the need to unwrite this “imagined Oceania,” while Māori author Keri Hulme writes disparagingly of the lower-cased ‘gods of literature’, and in her ReadNZ lecture, Sāmoan-Māori fantasy writer Lani Wendt Young describes traditional publishing as the “white castle of literature.” While white castled gods of literature have historically ignored the everyday vibrancy of Pacific voices, Indigenous writers from the Pacific continue to unapologetically write, read, experiment, critique, and play with words. Into this complexity, we acknowledge that for many Indigenous writers from the Pacific, English language Eurocentric thought and texts dominate our lives; many of us are second language learners of our languages.

year: 2024-08-24