title: Mouri Tu, Mouri Moko, Mouri Ora! Moko as a wellbeing strategy

authors: Penehira, Mera

subjects: Maori

abstract: This research has been undertaken within the Health Research Council funded International Collaborative Indigenous Health Research Partnership (ICIHRP) program, “The role of resiliency in responding to blood borne viral and sexually transmitted infections in Indigenous communities”. Some Indigenous communities in Australia, New Zealand and Canada have been shown to experience higher rates of blood borne viral infections. There are a number of categories of health intervention that have been shown to be effective in prevention and enhancing access to treatment for blood borne viral infections, including various forms of health promotion, enhanced diagnostic and treatment services, and harm reduction measures related to injecting drug use. The present research was undertaken with the understanding that development of effective responses of this kind among Indigenous people would benefit from a better understanding of the social and cultural factors that might protect against these infections and their consequences. It is argued that such factors are linked in various ways to Indigenous resistance and resiliency, which is described as the means by which people choose to make use of individual and community strengths to protect themselves against adverse health outcomes and enhance their health and wellbeing.

In this thesis I explore how people and their identity are affected when you are part of a marginalised or vulnerable population – namely, Māori women who have contracted the Hepatitis C Virus (HCV). I argue that traditional knowledge and healing practices are central to Māori getting well and keeping well, and that the use of cultural frameworks and practices have potentially restorative, therapeutic and healing values that are not yet researched or understood by the health field. I argue that a Māori framework of wellbeing, namely ‘Mana Kaitiakitanga’, provides the context in which tā moko (Māori traditional tattoo) fits naturally as a healing intervention. I share the stories of Māori women with HCV who have applied this (tā moko and other forms of tattoo) in their lives and in their journeys back to wellness. Tā moko is a process that penetrates the flesh and marks the skin; it is a process that involves both blood and pain, which may seem incongruous with healing. It is argued however that through pain comes understanding; through pain comes a RE-membering of strength; through pain comes joy; and finally through marking comes identity of who we are and how well we have been and can be again.

Issues and intersections of identity, marginalisation, gender, health, and wellbeing are at the forefront of this research story and of the journeys of the three women whose case studies are presented in this thesis. What makes this thesis unique is that it researches all three of these potential cornerstones of health: identity; wellbeing (spiritual and emotional), and physical wellbeing, in relation to a specific health problem, namely HCV. It is intended that this work will add power to what might be viewed as a particularly Indigenous solution to a virus that disproportionately affects our Indigenous population. This is not about rejecting Western health solutions, but it is about recognising and returning to Indigenous health solutions.

text: Mouri Tu, Mouri Moko, Mouri Ora! Moko as a wellbeing strategy This research has been undertaken within the Health Research Council funded International Collaborative Indigenous Health Research Partnership (ICIHRP) program, “The role of resiliency in responding to blood borne viral and sexually transmitted infections in Indigenous communities”. Some Indigenous communities in Australia, New Zealand and Canada have been shown to experience higher rates of blood borne viral infections. There are a number of categories of health intervention that have been shown to be effective in prevention and enhancing access to treatment for blood borne viral infections, including various forms of health promotion, enhanced diagnostic and treatment services, and harm reduction measures related to injecting drug use. The present research was undertaken with the understanding that development of effective responses of this kind among Indigenous people would benefit from a better understanding of the social and cultural factors that might protect against these infections and their consequences. It is argued that such factors are linked in various ways to Indigenous resistance and resiliency, which is described as the means by which people choose to make use of individual and community strengths to protect themselves against adverse health outcomes and enhance their health and wellbeing.

In this thesis I explore how people and their identity are affected when you are part of a marginalised or vulnerable population – namely, Māori women who have contracted the Hepatitis C Virus (HCV). I argue that traditional knowledge and healing practices are central to Māori getting well and keeping well, and that the use of cultural frameworks and practices have potentially restorative, therapeutic and healing values that are not yet researched or understood by the health field. I argue that a Māori framework of wellbeing, namely ‘Mana Kaitiakitanga’, provides the context in which tā moko (Māori traditional tattoo) fits naturally as a healing intervention. I share the stories of Māori women with HCV who have applied this (tā moko and other forms of tattoo) in their lives and in their journeys back to wellness. Tā moko is a process that penetrates the flesh and marks the skin; it is a process that involves both blood and pain, which may seem incongruous with healing. It is argued however that through pain comes understanding; through pain comes a RE-membering of strength; through pain comes joy; and finally through marking comes identity of who we are and how well we have been and can be again.

Issues and intersections of identity, marginalisation, gender, health, and wellbeing are at the forefront of this research story and of the journeys of the three women whose case studies are presented in this thesis. What makes this thesis unique is that it researches all three of these potential cornerstones of health: identity; wellbeing (spiritual and emotional), and physical wellbeing, in relation to a specific health problem, namely HCV. It is intended that this work will add power to what might be viewed as a particularly Indigenous solution to a virus that disproportionately affects our Indigenous population. This is not about rejecting Western health solutions, but it is about recognising and returning to Indigenous health solutions.

year: 2011

title: Think globally, act locally: Collective consent and the ethics of knowledge production

authors: Hudson, Maui

abstract: Ethical review is an integral part of the process of developing research and considering issues associated with the production of knowledge. It is part of a system that primarily legitimises western traditions of inquiry and reinforces western assumptions about knowledge and its benefit to society. Around the world the process of colonisation has excluded indigenous understandings. In New Zealand, Māori (indigenous) knowledge has been similarly marginalised; this pattern is also reflected within ethical review. Māori values, while acknowledged, are not yet considered to have equal weight in ethical deliberations. The notion of collective rights and the possibility of developing processes to allow collective consent to be recognised and mandated by ethics committees have been raised by communities but largely ignored by the ethical review system. While kaupapa Māori researchers espouse the benefits of closer community involvement, policy makers and ethics committees have focused on “consultation” as the mechanism which confirms proof of engagement, the establishment of community support, and the relevance of the project. This article highlights the potential of the concept of collective consent in negotiations between researchers and communities.

text: Think globally, act locally: Collective consent and the ethics of knowledge production Ethical review is an integral part of the process of developing research and considering issues associated with the production of knowledge. It is part of a system that primarily legitimises western traditions of inquiry and reinforces western assumptions about knowledge and its benefit to society. Around the world the process of colonisation has excluded indigenous understandings. In New Zealand, Māori (indigenous) knowledge has been similarly marginalised; this pattern is also reflected within ethical review. Māori values, while acknowledged, are not yet considered to have equal weight in ethical deliberations. The notion of collective rights and the possibility of developing processes to allow collective consent to be recognised and mandated by ethics committees have been raised by communities but largely ignored by the ethical review system. While kaupapa Māori researchers espouse the benefits of closer community involvement, policy makers and ethics committees have focused on “consultation” as the mechanism which confirms proof of engagement, the establishment of community support, and the relevance of the project. This article highlights the potential of the concept of collective consent in negotiations between researchers and communities.

year: 2010

title: Towards identification of floc compounds in water using multi-frequency fluorescence lifetime analysis

authors: Dissanayake, S.A.D. Asanka Nilakshi

subjects: Fluorescence lifetime

abstract: UV disinfection is commonly used in water treatment to inactivate pathogens such as Cryptosporidium and viruses to prevent diseases such as cryptosporidiosis and norovirus in communities. Disinfection typically follows water treatment steps, such as coagulation, flocculation, clarification, and filtration. However, particles in water, for example, flocs 0.1 to 100 μm in diameter, made from humic and inorganic substances present in the water, surrounding a Cryptosporidium oocyst or virus, can protect the pathogens from UV exposure. Although water treatment steps prior to disinfection remove 99% of the particulates, particles can still be present in the 1000s to 10,000’s per litre after filtration. While the chances of a floc particle carrying a virus or oocyst are typically low, in some regions, particularly during calving in the dairy industry, oocyst concentrations in the water might be high due to cryptosporidiosis in calves. Therefore, it is useful to test the properties of the floc compound for UV penetration to determine whether the disinfection method is appropriate.

In this thesis, a technique that uses multi-frequency analysis to measure the fluorescence lifetime of a fluorophore to provide information on particle composition
is presented. Frequency-domain fluorescence fluorometry was used to determine the fluorescence lifetime. This was achieved using an experimental setup that used a laser diode operating at 100 mW and modulated at 10–60 MHz to excite the fluorophores, optical elements to focus and filter the light, and detectors to collect the fluorescence emission signal via a storage oscilloscope. The signals were then processed using a MATLAB program to determine the fluorescence lifetime. 

Fluorescence lifetime measurements were challenged by the chemical and physical behaviour of the fluorophore and the adsorption of the fluorophore to the floc particles. Therefore, standard measurements such as turbidity, pH, particle size, and fluorescence were used to understand the absorption/adsorption of fluorescein to flocs. Fluorescence was observed at the 260–490 nm excitation wavelengths, with fluorescence emissions at approximately 510 nm. The particle size and turbidity measurements showed that fluorescein acted as a flocculant, with the particle size increasing with increasing fluorescein concentration. Fluorescence intensity measurements from a standard fluorescence spectrophotometer were used to calculate fluorescein adsorption on humic acid and kaolin to generate adsorption isotherms. Fluorescein was bonded to kaolin 10 times more than to humic acid. Adequate flocculation required a pH of 6.5 to produce reasonably flocculated particles in the sample. Surface charge analysis showed that the use of buffer to control pH required more alum to neutralise the surface charge of humic acid and kaolin.

Multi frequency measurements and subsequent analysis showed that the fluorescence lifetime and contamination ratio were 4.2 ± 0.3 ns and 0.09 ± 0.05 for fluorescein. The fluorescence lifetime of fluorescein was compatible with the results of previous studies using different techniques. The samples with floc particles had a larger excitation light contamination ratio than those without particles; therefore, the contamination ratio could be used as a measure of particle contamination in the samples. The fluorescence lifetime of fluorescein did not change when fluorescein was attached to humic acid particles, but increased by 0.6 ns for kaolin floc particles.

text: Towards identification of floc compounds in water using multi-frequency fluorescence lifetime analysis UV disinfection is commonly used in water treatment to inactivate pathogens such as Cryptosporidium and viruses to prevent diseases such as cryptosporidiosis and norovirus in communities. Disinfection typically follows water treatment steps, such as coagulation, flocculation, clarification, and filtration. However, particles in water, for example, flocs 0.1 to 100 μm in diameter, made from humic and inorganic substances present in the water, surrounding a Cryptosporidium oocyst or virus, can protect the pathogens from UV exposure. Although water treatment steps prior to disinfection remove 99% of the particulates, particles can still be present in the 1000s to 10,000’s per litre after filtration. While the chances of a floc particle carrying a virus or oocyst are typically low, in some regions, particularly during calving in the dairy industry, oocyst concentrations in the water might be high due to cryptosporidiosis in calves. Therefore, it is useful to test the properties of the floc compound for UV penetration to determine whether the disinfection method is appropriate.

In this thesis, a technique that uses multi-frequency analysis to measure the fluorescence lifetime of a fluorophore to provide information on particle composition
is presented. Frequency-domain fluorescence fluorometry was used to determine the fluorescence lifetime. This was achieved using an experimental setup that used a laser diode operating at 100 mW and modulated at 10–60 MHz to excite the fluorophores, optical elements to focus and filter the light, and detectors to collect the fluorescence emission signal via a storage oscilloscope. The signals were then processed using a MATLAB program to determine the fluorescence lifetime. 

Fluorescence lifetime measurements were challenged by the chemical and physical behaviour of the fluorophore and the adsorption of the fluorophore to the floc particles. Therefore, standard measurements such as turbidity, pH, particle size, and fluorescence were used to understand the absorption/adsorption of fluorescein to flocs. Fluorescence was observed at the 260–490 nm excitation wavelengths, with fluorescence emissions at approximately 510 nm. The particle size and turbidity measurements showed that fluorescein acted as a flocculant, with the particle size increasing with increasing fluorescein concentration. Fluorescence intensity measurements from a standard fluorescence spectrophotometer were used to calculate fluorescein adsorption on humic acid and kaolin to generate adsorption isotherms. Fluorescein was bonded to kaolin 10 times more than to humic acid. Adequate flocculation required a pH of 6.5 to produce reasonably flocculated particles in the sample. Surface charge analysis showed that the use of buffer to control pH required more alum to neutralise the surface charge of humic acid and kaolin.

Multi frequency measurements and subsequent analysis showed that the fluorescence lifetime and contamination ratio were 4.2 ± 0.3 ns and 0.09 ± 0.05 for fluorescein. The fluorescence lifetime of fluorescein was compatible with the results of previous studies using different techniques. The samples with floc particles had a larger excitation light contamination ratio than those without particles; therefore, the contamination ratio could be used as a measure of particle contamination in the samples. The fluorescence lifetime of fluorescein did not change when fluorescein was attached to humic acid particles, but increased by 0.6 ns for kaolin floc particles.

year: 2025