title: A simplified protocol for detecting two systemic bait markers (Rhodamine B and iophenoxic acid) in small mammals

authors: Purdey, D. C.

subjects: stoat

abstract: We developed a method of quantifying levels of fluorescence in the whiskers of wild stoats (Mustela erminea) using fluorescence microscopy and Axiovision 3.0.6.1 software. The method allows for discrimination between natural fluorescence present in or on a whisker, and the fluorescence resulting from the ingestion of the systemic marker Rhodamine B (RB), although some visual judgement is still required. We also developed a new high performance liquid chromatography (HPLC) protocol for detecting the systemic marker iophenoxic acid (IPA) in the blood of laboratory rats (Rattus norvegicus) and wild stoats. With this method, the blood of an animal that has consumed IPA can be tested for the presence of the foreign IPA compound itself. This is a more reliable test than the previous method, which measured the raised level of natural blood protein-bound iodine correlated with IPA absorption. The quantity of blood required from animal subjects is very small (10 μl), so the testing is less intrusive and the method can be extended to smaller species. The extraction technique uses methanol, rather than acids and heavy metal salts, thereby simplifying the procedure. Recovery of IPA is quantitative, giving a highly reliable reading. In experiments on captive rats the IPA method proved successful. Of 12 positively marked carcasses, two that had not been frozen for the 24 h before blood samples were taken showed relatively lower IPA levels. The same IPA detection method, as well as the whisker analysis for RB, was applied successfully to a population of wild stoats to which both Rhodamine B and IPA were made available at bait stations. The presence of both bait markers was detectable in rats for at least 21 days and in stoats for at least 27 days.

text: A simplified protocol for detecting two systemic bait markers (Rhodamine B and iophenoxic acid) in small mammals We developed a method of quantifying levels of fluorescence in the whiskers of wild stoats (Mustela erminea) using fluorescence microscopy and Axiovision 3.0.6.1 software. The method allows for discrimination between natural fluorescence present in or on a whisker, and the fluorescence resulting from the ingestion of the systemic marker Rhodamine B (RB), although some visual judgement is still required. We also developed a new high performance liquid chromatography (HPLC) protocol for detecting the systemic marker iophenoxic acid (IPA) in the blood of laboratory rats (Rattus norvegicus) and wild stoats. With this method, the blood of an animal that has consumed IPA can be tested for the presence of the foreign IPA compound itself. This is a more reliable test than the previous method, which measured the raised level of natural blood protein-bound iodine correlated with IPA absorption. The quantity of blood required from animal subjects is very small (10 μl), so the testing is less intrusive and the method can be extended to smaller species. The extraction technique uses methanol, rather than acids and heavy metal salts, thereby simplifying the procedure. Recovery of IPA is quantitative, giving a highly reliable reading. In experiments on captive rats the IPA method proved successful. Of 12 positively marked carcasses, two that had not been frozen for the 24 h before blood samples were taken showed relatively lower IPA levels. The same IPA detection method, as well as the whisker analysis for RB, was applied successfully to a population of wild stoats to which both Rhodamine B and IPA were made available at bait stations. The presence of both bait markers was detectable in rats for at least 21 days and in stoats for at least 27 days.

year: 2003-09-01

title: Beyond shrek: fairy tale magic in the multicultural classroom

authors: Sturgess, Janet

subjects: English literature

abstract: This article draws on a recent research project in New Zealand on teaching literature in the multicultural classroom. Its focus is on how one teacher-researcher used the cultural and linguistic diversity of a junior, secondary English class as a resource in the development of her programme. It details a sequence of activities designed for a unit of work on the critical reading and composition of fairy tales, viewed as a popular genre across all cultures, and the impact of these activities on student participants. A range of pedagogical strategies are identified as successful: viewing cultural and linguistic diversity as a resource, empowering students to contest textual meaning, modeling, task-based inquiry and whole-class activity.

text: Beyond shrek: fairy tale magic in the multicultural classroom This article draws on a recent research project in New Zealand on teaching literature in the multicultural classroom. Its focus is on how one teacher-researcher used the cultural and linguistic diversity of a junior, secondary English class as a resource in the development of her programme. It details a sequence of activities designed for a unit of work on the critical reading and composition of fairy tales, viewed as a popular genre across all cultures, and the impact of these activities on student participants. A range of pedagogical strategies are identified as successful: viewing cultural and linguistic diversity as a resource, empowering students to contest textual meaning, modeling, task-based inquiry and whole-class activity.

year: 2009

title: New observations on the stratigraphy and radiocarbon dates at the Cross Creek site, Opito, Coromandel Peninsula

authors: Furey, Louise

subjects: New Zealand

abstract: This paper re-examines stratigraphy and radiocarbon dates at Cross Creek in Sarah's Gully. Three new radiocarbon dates are presented for Layer 9, the earliest, and previously undated, occupation. This investigation is part of a programme of archaeological work being carried out on the Coromandel Peninsula. Although there are several individual research projects underway, they have a common theme related to the Polynesian settlement period on the Coromandel Peninsula. The two seasons of excavation at Tairua are being written up by Matthew Campbell of CFG Heritage on behalf of Roger Green. Louise Furey, also CFG Heritage, is researching a thematic study on early sites for the Department of Conservation, Archaeological research in the Opito area includes documenting a pollen sequence for Opito under a grant obtained from the Green Foundation for Polynesian Research: Pam Chester, Louise Furey and Brenda Sewell are participants. In addition, positively identifying the Kaharoa Ash in the Opito-Sarah's Gully area is a priority.

text: New observations on the stratigraphy and radiocarbon dates at the Cross Creek site, Opito, Coromandel Peninsula This paper re-examines stratigraphy and radiocarbon dates at Cross Creek in Sarah's Gully. Three new radiocarbon dates are presented for Layer 9, the earliest, and previously undated, occupation. This investigation is part of a programme of archaeological work being carried out on the Coromandel Peninsula. Although there are several individual research projects underway, they have a common theme related to the Polynesian settlement period on the Coromandel Peninsula. The two seasons of excavation at Tairua are being written up by Matthew Campbell of CFG Heritage on behalf of Roger Green. Louise Furey, also CFG Heritage, is researching a thematic study on early sites for the Department of Conservation, Archaeological research in the Opito area includes documenting a pollen sequence for Opito under a grant obtained from the Green Foundation for Polynesian Research: Pam Chester, Louise Furey and Brenda Sewell are participants. In addition, positively identifying the Kaharoa Ash in the Opito-Sarah's Gully area is a priority.

year: 2008