A Scalable Bio-based Solution to Eliminate Cyanotoxins in Drinking Water One of the greatest global challenges facing human-kind is access to reliable safe drinking water. This is particularly acute in developing countries where human activities significantly impact water quality. While the Earth is known as the blue planet with 71% of the surface covered by water, most of this is seawater and not suitable for human consumption, industrial applications or agriculture, all of which are essential for socioeconomic development. Only a tiny proportion of the Earth's water is freshwater (~3%) but <1% is available for use. This small amount of available water is under strain and a recent UN report predicted that 5 billion people could suffer water shortage by 2050 as a consequence of climate change, increased demand and pollution. One serious threat to water quality and public health is the occurrence of blooms of cyanobacteria (blue-green algae) as a result of nutrient pollution (nitrate and phosphate) from industry, agriculture and domestic waste. Cyanobacteria produce dangerous toxins (cyanotoxins) causing acute and chronic symptoms leading to fatalities, most notably in Caruaru, Brazil with over 71 fatalities and cancer humans, including primary liver cancer documented in China. In addition, there is concern that these toxins may be responsible for rising cases of chronic kidney disease of unknown origin (CKDu) in Asian countries such as Sri Lanka where cyanotoxins can be perennial. These toxins are very stable and not removed by typical drinking water treatment processes or even boiling, therefore an innovative, simple and sustainable solutions are needed for their removal. Professor Pathmalal Manage, (University of Sri Jayewardenepura, Sri Lanka) along with Dr Christine Edwards & Professor Linda Lawton (both of the Aberdeen Industrial Biotechnology Institute, RGU) have demonstrated the effectiveness of microbial populations for safe elimination of these toxins from water. They have found that natural microbial consortia, even with no previous exposure to specific toxins, contain active biodegraders. Degradation is promiscuous meaning that, for example, exposure to peptides will enrich the microbiome with consortia members with the ability to eliminate microcystins (heptapeptide cyanotoxins). Furthermore, there is evidence that some naturally occurring microbes can actively kill toxin-producing species of cyanobacteria. This project aims to harness and stimulate this microbial capability, naturally immobilised on biochar to provide a scalable water treatment system that can be used at all magnitudes from rural wells through to municipal water treatment facilities in Sri Lanka. The biochar will be developed to provide a low cost support for optimized microbiomes and will be produced locally from biogenic waste (e.g. coconut husks/shells, rice straw) exploiting the global expertise of Dr Ondrej Masek, of the UK Biochar Research Centre (UEd). Ensuring that water is rendered free from harmful concentrations of cyanotoxins a simple field kit will be developed using antibodies that react to multiple classes toxins, led by Dr Katrina Campbell (QUB) with world leading expertise on development of innovative diagnostics for a wide range of toxins. On completion of the research, the project will provide low cost, simple, scalable, nature-based water treatment systems for elimination of commonly occurring toxins from cyanobacteria. In addition, local communities will benefit from better use of agricultural waste to produce biochar which can be useful for a wide range of applications such as fertiliser with the added bonus of gas generation as it is produced providing an alternative energy source. This water treatment solution will have wide application in many developing countries and will contribute to achieving UN SG6 while embracing the philosophy of the United Nations World Water Development Report which emphasises the benefits of 'Nature-Based Solutions for Water.' The Global Challenges Research Fund (GCRF) supports cutting-edge research to address challenges faced by developing countries. The fund addresses the UN sustainable development goals. It aims to maximise the impact of research and innovation to improve lives and opportunity in the developing world. The fund addresses the UN sustainable development goals. It aims to maximise the impact of research and innovation to improve lives and opportunity in the developing world.