Sentence:  With this purified conjugate the best staining procedure consisted of treating the sections with <WSD>1</WSD> : 10 dilution of * * f for 30 minutes , washing with PBS for 15 minutes , staining with * * f for 30 minutes , and washing with PBS for 15 minutes . 
Meaning of the word marked with <WSD> tags:  used of a single unit or thing; not two or more