Sentence:  These cells were thawed at 37 ` C for 30 min and were deglycerolized by alternately centrifuging and mixing with <WSD>descending</WSD> concentrations of glycerol solutions ( 20 , 18 , 10 , 8 , 4 and 2 % ) . 
Meaning of the word marked with <WSD> tags:  move downward and lower, but not necessarily all the way