Sentence:  Moreover , after concentration <WSD>using</WSD> negative pressure dialysis , agglutinin activity could sometimes be detected in the region designated 2a ( donors P. J. , D. A. , and J. F. , Fig. 1 ) . 
Meaning of the word marked with <WSD> tags:  put into service; make work or employ for a particular purpose or for its inherent or natural purpose