Sentence:  Sections of sweet clover stem and root tumors were treated with 1 : 10 solution of * * f for 30 minutes , washed in <WSD>buffered</WSD> saline for 15 minutes , stained with * * f for 30 minutes , and washed for 15 minutes in PBS . 
Meaning of the word marked with <WSD> tags:  add a buffer (a solution)