Sentence:  These cells were thawed at 37 ` C for 30 min and were deglycerolized by alternately centrifuging and <WSD>mixing</WSD> with descending concentrations of glycerol solutions ( 20 , 18 , 10 , 8 , 4 and 2 % ) . 
Meaning of the word marked with <WSD> tags:  mix together different elements