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COMMISSION OFTHEEUROPEAN COMMUNITIES
COM(78)294final.
Brussels .28.June1978,
ReporttoCouncilandParliament under>
Article2ofCouncilDecision(72/446/CEE)
of28thDecember 1972adopting acommon
research programme inthefieldofClassical
■V.•/andAfrican SwineFever V*///v //X/i\V*
/GEE):,-,
on ., J
Classical o"y
*/*i
(presented "bytheCommission )
CC?.:(78)294final.

-:MMISSION CFTHBEUROPEAN COMMUNITIES
Directorate General forAgriculture .
Coordination ofAgricultural Research
Report toCouncil andParliament
under Article 2ofCouncil Decision N° (72/446/CEE)
of28th December 1972 adopting acommon research programme
inthe field ofClassical and African Swine Fever
INTRODUCTION
-Thefirst coaanon programme ofresearch onClassical (HogCholera )and
African Swine fever covered theperiod 1964-1971 .Itsbasic objec­
tives were :
thephysical andchemical .properties ofthevirus ,theinfluence
ofphysical andchemical factors onitsstability ,thephysical
andchemical properties ofthespecific viral proteins ,andthe
structural andserological relations with other viruses for
.purposes ofclassification ;
thebiological properties (1)ofthevirus with special reference
tocellular systems capable ofensuring viral growth ,methods of
assaying infection ,serological methods ofidentification ,viral
multiplication (1)ofiaterferoa andtherelation between the
1 -V
properties orthevirus anditsvirulence totheanimal ;
thesetting upofdifferential diagnosis between Classical (Hog
Cholera )andAfrican Swine fever from thedifferent techniques
used fortheclinical' diagnosis andthemacroscopic examination
ofthelesions ,thehisto-pathological analysis andtheviro-
logies ],a&dserological diagnosis .
(I)invitro
/

-2-
Theresults ofthisprogramme werepublished inOctober 1971
under theauspices oftheGeneral Directorate forAgriculture
under thetitle :"Properties ofthevirus ofClassical swine
fever anddifferential diagnosis ofClassical andAfrican Swine
. (
fevers ". -
Thegeneral approach tothediagnosis oftheClassical (HogCholera )
swine fever included field examinations which could identify a
suspected outbreak pending laboratory confirmation .
FortheAfrican swine fever theapproach wasthesame .However ,in
order toavoid confusiofl atthediagnostic level *adistinction
wasmade between countries where both diseases existed andthose
where only Classical (HogCholera )swine fever waspresent .In
i
both cases ,butmore particularly inthelatter ,laboratory
diagnosis waswidely used toobtain 4reliable confirmation of 1
thepresence ortheabsence ofAfrican Swine fever .Infact ,at
the'beginning oftheprogramme ,thetypical lesions resulting for
theAfrican swine fever disease were frequently observed ;but ,
subsequently ,numerous atypical lesions akin totheClassical
(HogCholera )swine fever appeared .Hence differential diagnosis
based onanatomical examinations ,proved unreliable .During the
Seminar ,organised 'bytheCommission inLisbon in1971 ,itwas .
suggested that subsequent efforts should concentrate onimproving
laboratory diagnosis . 1
Thework initiated inthefirst programme wascontinued inthe
second programme (1973-1976 )which isthesubject ofthis report ,
andwhich wasimplemented following theCouncil approval in1972
oftheCommission proposed programme ofresearch ontwoenzootic
viruses ofthepig ,theClassical andAfrican swine fever viruses
(2).Thecontent :oftheprogramme wasselected to .meet specific

objectives ofthecommon agricultural policy andinparticular to
minimize theeconomic losses caused bythese diseases andto
promote thetrade objective ofthecommon agricultural policy (3).
RESEARCH ACTIVITIES
a)General
' • . ;:
Thetask tocarry outresearch onSwine Fever Viruses wasgiven
.■ ]
toseventeen laboratories ofdifferent research institutes (Annex I)
ofwhich fifteen dealt with Classical swine fever andfive (in
Spain andPortugal andsubsequently theUnited Kingdom France and
Denmark )with African swine fever .Several laboratories ,
(intheUnited Kingdom ,Denmark andIreland )joined during the
» •
second phase oftheprogramme (4). ,
' ι .-' · „ ·' "/
Anewapproach inthecoordination ofresearch activities seemed
necessary toovercome certain difficulties encountered inthe
/ -
first phase oftheprogramme .Toenable acloser collaboration
between theparticipating laboratories ,theCommission invited
scientists ofinternational standing toassist itbygiving
Xadvice onthescientific content andprogress oftheprogramme .
(2)Decision (*2/446/C£E)of28December 1972/O.J.N®298/50
(3)Themain economic losses intheCoaaiimity were been caused bythe
Classical swine fever virus .TheAfrican swine fever virus is
present iaSpain endPortugal ,andrepresents aconstant threat to
theCommunity .
(4)Luxembourg does nothave anational agricultural research service .
Itworks directly iacooperation «lthother Member States .

Seminars andWorkshops were organised bylaboratories inthe
Member States attheinvitation oftheCommission andafter
discussion within theprogramme working groups iThehost labo­
ratory made thenecessary local arrangements ,circulated papers ,
andassisted theCommission inthechoice ofspeakers .
».
Exchange ofscientists between laboratories andvisits ofscien­
tists fortraining orinformation ,were arranged onanad-hoc
basis .Such exchanges andvisits were essential toensure full
cooperation between laboratories andadequate access toinformation .
Areport wasrequired from each scientist benefitting from these
grants .
Theproceedings ofseminars andworkshops have been published in
*
conformity with theCommission 'spublication policy .Annex III
!
provides thelist ofpublications resulting from theresearch
activities . (
Main scientific objectives 1
•1 Ι
Themain scientific objectives of'theprogramme were toisolate
theAfrican Swine Fever Virus ,(ASFV )andClassical Swine Fever
Virus (CSFV )andtoisolate andcharacterize "their protein and
nucleic acids .Theavailability ofantigenic fractions ofthese
viruses wastolead tothedevelopment ofdiagnostic procedures
forquick andaccurate detection ofthevirus .-Theimmune response
ofthepigwould beanalysed andvaccines tested .Inaddition
theCommission gave another dimension totheprogramme ,conceiving
itasatool forpromoting collaboration between scientists of
different countries oftheCommunity ,andformaking sure that the
programme would result inpractical achievements utilized directly
forthebenefit offarmers . .

-5"
c)Exchange ofscientists
Visit *tolaboratories bytheCommission 'sscientific adviser and
theexchange oftechniques bymeans ofexchange ofpersonnel
shortened thetime required forstandardization oftechniques in
allthelaboratories working onthesame subject ,andforthe
;evaluation ofresults .Work onASFV wascarried outinsecurity
M.1
laboratories ,andwasrestricted totheIberian laboratories
(Madrid andLisbon )andPirbright laboratory .Itwasimperative
that scientists from countries' immune toASFV should work in
protected laboratories (5).Thetravel expenses ofscientists were^
paid bytheCommission .
d)Exchange ofmaterials
Theprogramme wasalso successful indeveloping inter-laboratory
relationships ,resulting inthetransfer ofvirus samples from
onelaboratory toothers (with thetechniques necessary for
purification andisolation ofthevirus aswell asforitsanalysis ).
Exchange ofVirus strains andcell-lines between thedifferent
laboratories wasessential tothedevelopment oftheprogramme .
(5)Toovercome thedifficulties indeveloping theresearch onASFV
which wascarried out inconfined laboratories and therefore
where exchange ofmaterials wasnotpossible andexchange of •
scientists wasnoteasy ,ascientific task group which included
experts iniridovirus research ,inelectron ssicroscopy ,inbio­
chemistry onmolecular virology andiavirology andcell biology
wasconvened iatheMadrid Institute working onASFV .~

e)Seminars andWorkshops
Discussions with scientists indifferent laboratories indicated
considerable interest incollaboration among scientists working
inthesame field ofresearch .Certain laboratories haddeveloped
'· \
techniques which could bevery useful asstandard procedures .For
this reason ,emphasis wasgiven toorganising workshops and
"seminars toenable dissemination ofinformation .
Alltheworkshops took place in1975 .Seminars were held onan
annual basis ,oneperyear .They were organized inorder to
bring together scientists ofeach participating laboratory ,as
well asscientists from third countries .Theseminars included
oral presentations ofresearch results anddiscussions ofcurrent
results and future needs .
;Theparticipants were divided into several discussion groups .
Their proposals andconclusions were brought before theplenary ^
meeting which adopted 'final suggestions forpresentation tothe
Commission services andtheStanding Committee onAgricultural
t / » . -
Research (CPRA)(6). ii8tofseminars andworkshops organised
isattached inAnnex II. ^
i;.. .' ■ '
f)Harmonization ofveterinary legislation ,
Closer co-operatidn with theCommission 'sservices responsible
foragricultural legislation inveterinary matters soon appeared
necessary .Scientists ,especially those involved intheepide - :
miology ofswine fever virus andtheappearance anddisappearance
ofepidemics ,felt that thelegislators could andshould benefit
from thescientific knowledge accumulated inthefield during
many years . r ,
(6)Council Regulation N°(EEC)1728/74of27Jijne 1974 ,O.J. N°182/1.

-7-
Asaresult ,veterinary legislation experts participated inthe
seminars andworkshops which dealt with epidemiology anderadica -
i
tion ofswine fever viruses .
i . ■_
Inaddition ,aspecial Committee wasestablished ,including
several senior scientists andmembers oftheveterinary legis-'
v .
lation services ,todiscuss speicific policy measures foreradi­
cation ofswine fever diseases .Also ,asurvey wascarried out
on"Studies ontheepidemiology andeconomics ofswine fever
eradication intheEEC".This study wasfinanced bytheresearch
programme onswine fever viruses . . .
g)Finance
- :... » .
Atotal of2.723,003 u.a .wasallocated bytheCouncil tofinance
theprogramme ,ofwhich 2.450.503 u.a .forresearch activities
♦*
and272.500 u.a .forco-ordinated activities .The research acti­
vities component corresponded to50%ofthetotal cost ,the
other 50%being provided bytheparticipating institutes .The -
co-ordinated activities were financed bytheCommunity infull .
Attheendoftheprogramme ,approximately 80%oftheoriginal
rcredit allocation wasspent .Theunused difference reflects
partly strict management practices bytheCommission ,andpartly
thefact that oneofthemajor contractors (I'INIA PORTUGAL )'
could notbeiicplemented ,duetopolitical events inPortugal in
thespring of1975 . 7
Anfiex VIgives theexpenditure figures oftheprograms .

**8
RESULTS
I. Classical swine fever virus
Theisolation ofthevirus andtheanalysis ofitsproteins and
^Ribonucleic acid (RNA)wereachieved .Itisnowknown thatoneof
theviral proteins istheantigen identical toanantigen in
Bovine Virus Diarrhoea (BVD).Thesecond protein ofclassical
svine fever isunique tothis virus .Inorder toutilize this
knowledge andputittopractical usefurther workisnecessary .
Theaimistousetheunique protein ofCSFV inaradioimmuno­
assay which willmake itpossible todetect specific antibodies
tothat virus inpigs .With theavailable techniques BVDinfec­
tion canbedetected instead ofclassical swine fever infection .
BVDinfection ofswine does notcause anydeleterious effect ,
while theantibodies tothis virus interfere with thedetection
ofclassical swine fever virus infection .Thedevelopment ofa
radioimmunoassay toselectively detect antibodies toclassical
swine fever virus will make possible theearly diagnosis ofvirus
infection inyoung animals andbreeding sows .
1
Theprograntm provided useful information onthenature ofvaccine
strains available aswell asontheconditions forimproved
vaccination with these vaccine virus strains ..Inaddition ,
special "attention wasgiven totheimmune system ofthepig ,
which differs significantly from that ofother animals .New
information ontheepidemiology ofthis virus infection was
obtained .Finally ,many newareas ofresearch onthegenetics of
this virus ,theviral nucleic acid andproteins aswell asonthe
immune response intheyoung animal were opened asaresult of
• *
theprogramme . i j

II.African swine fever virus
Though only fewlaboratories were engaged inresearch inthis
field ,significant developments have occurred during thelast
– ■ r
four years .
Thepurification ofASFVwasachieved andledtotheanalysis o^
'' .•theviral Desoxy ribonucleid acid (DNA)andstructural proteins .
Initial information onthemode ofvirus replication wasobtained .
Soine oftheviral antigens were isolated andpartially identified .
Many fields ofresearch were opened asaresult ofthis activity
including abetter understanding oftheepidemiology ofthis „
virus disease which threatens tospread from theIberian peninsula .
CONCLUSIONS
Thegeneral ,conclusion tobedrawn isthat this programme hasprovided
some basic knowledge onthetwoswine fever viruses andthat ithas
opened thewaytopractical future achievements in-thefields of
diagnostic procedures andinthedevelopment ofbetter field control
measures . I
More specifically ,,theprogramme hasdemonstrated that :
* although reliable data isstill sparse ,aview seems toemerge
that acycle oflowvirulence virus infection canbeestablished
inbreeding herds .Theconditions inwhich this occurs cannot be
fully define *?until further evidence onthecharacteristics of
herds infected inthis wayisobtained from field testing activi -
<ties nowiaprogress .Itissuggested that' large self contained
herds producing their o*mreplacements aadmedium sized herds in
areas with dense pigpopulations .wfosre there areregular introductions

-10-
offresh breeding stock ,aridmovements ofpiglets ,eremost at
risk ofdeveloping such acycle .Thesituation inthese herds
could belikened toafire which isregularly receiving fresh
fuel tokeep itburning .Theexact mechanism oftransmission in
these herds isnotyetclear ,butprobably dependent oncontact
between piglets carrying thevirus ,andnewly entered gilts or
young sows .
Detailed analysis ofthedata from affected herds andcomparisons
with thecharacteristics ofunaffected herds andareas isalmost
certain toreveal thekeyfactors that areresponsible forthe
development ofthecarrier herd cycle .Theprevalence ofsuch
/
herd infections appears low (5%)from work inthemost ,frequently
affected districts ofLower Saxony ,buttheproportion ofpigs
showing serological evidence ofinfection within affected herds
ishigh (40%).Findings insimilar work inFrance have revealed
positive serological reactions in46%ofasmall number ofsows
insuspect herds with reproductive problems .This evidence ,
added toobservations onthesmall numbers ofpiglets inaffected
litters that arecapable ofspreading thevirus ,aswell asthe
timing ofinfection inpregnancy necessary toproduce this phenomenon ,
suggest that theproportion ofinfective piglets produced inan
affected herd must besmall .
\
Although reliable field evidence iseven more sparse andvirological
evidence onthestability ofswine fever virus strains issomewhat
contradictory ,itdoes appear that virus from carrier pigs can
produce theclassical form ofclinical swine fever .However ,the
development ofthis overt disease inaherd maytake several
months ,which suggests that theacquisition ofnormal virulence
depends onsuccessive passages inAnumber ofsusceptible pigs in
the herd .

-11
- Forthereasons outlined itseems unlikely that vaccination can
overcome thecarrier cycle inbreeding herds ..Itshould appre­
ciably reduce therisk ofthis phenomenon developing but ,unfor­
tunately ,italso reduces thepossibilities ofdetecting the
presence ofcarrier infection byserological tests ., x
V ', . . *.
Thepotential forspread ofswine fever bydirect contact ishigh
because oftherapid turnover inpigherds ,intensity ofproduc­
tion systems ,density ofpopulations andcomplexity ofmarketing
systems .However ,ithasbeen shown that ifthechain ofcontacts
canbefollowed quickly andthoroughly itispossible tocontain
.theinfection before large numbers ofherds areinvolved .Iden­
tification ofpigs andregistration ofmovements are ,therefore ,
thefirst priority inaprevention programme BOthat when control ;
becomes necessary quarantine andother measures canhave maximum
■effect ,i
Information ontherisk of'spread byindirect contact israther
contradictory .Thevirus appears quite resistent butitis
generally accepted that therisk from surviving virus inthe
environment isfairly easily eliminated bygood hygiene ,raana-
\
gement andreadily acceptable official controls .
Theproblem ofswill varies with national food habits which
determine thepossibilities ofuncooked pork gaining entry topig
feed .Experience indicates that therisks ofintroducing swine
fever from heavily infected countries issuch that such importa­
tions arehard tojustify .However ,this risk canbeminimised
byeffective control over thecooking ofswill tobefedtopigs .
Annex IVprovides schismatic information onthescientific problems
existing atthebeginning oftheprograajtae .
Annex Vprovides information onthedifferent subjects dealt with
within theprograoa® «»».<!theresult® achieved . ■• ' '

ANNEXI
LISTOFPARTICIPATING INSTITUTES INTHECOMMONPROGRAMME ONCLASSICAL AND.AFRICANSWINEFEVER
SUBJECT INSTITUTS TITLEOFTHEPROJECT
I
Classical SwineFeverVirus
(C.S.F.V.)'Bundesforschungsanstalt fürViruskrankheiten der
Tiere-TÜBINGEN(Fed.Rep.ofGermany)Organization-ofC.S.F.V.
InstitutfürVirologiederTierarztlichen Hochschule
-Hannover(Fed.Rep.ofGermany)OrganizationofC.S.F.V.
Pathogenesis ofsubclinical HogColera
r • .» "■. ,. IInstitutNationaldeRecherches Vétérinaires -
UCCLE(Betgium) .' .■.Studiesonthepropertiesofthevirus
•• -Î-* StudiesonVirusvacine.
StateVeterinaryInstituteforVirusresearch-
LINDHOLM-KALVEHAVE (Denmark) >-^Studiesonthe"CommonAntigensof
swinefeverandBVDviruses.
CSFvaccine
DiagnosisofC.S.F.V.
PatnogenisofC.S.F.V.
!LaboratoireCentraldeRecherchesVétérinaires-
;MAISONSALFORT(France)..StudiesonC.S.F.V.
Classical Swinefevervaccine.
Laboratoire dePathologie Porcine,Stationde
-Recherches deVirologieetd'Immunologie-
THIVERVAL-GRIGNON (France)
(• •;StudiesonC.S.F.V.
Antibodies responsetoC.S.F.V.
Studyoftheimmunoglobulins .
.'IStudyofimmunogenicity .

INSTITUTE
Veterinary ResearchLaboratory,ABBOTSTOWN-
CASTLENOCK CoDublin(Ireland)
Instituto ZooprofilatticoSperimentale della
Lombardia edell'Emila~BRESCIA (Italy)
Instituto ZooprofilatticoSperimentale dell*
Abruzzo,TERAMO(Italy)
InstitutoZooprofilattico Sperimentale dell*
UmbriaedelleMarche-PERUGIA(Italy)
CentraalDiergeneeskundig Institut,Afdeling
Virologie-LELYSTAD .(TheNetherlands )
Institute ofVirology,Veterinary Faculty
'UTRECHT "(TheNetherlands )
VirologyDepartment,CentralVeterinary
Laboratory ~WEYBRID6E (U.K.)TITLEOFTHEPROJECT
Serology ofBovinevirusdiarrhoea
Studies onC.S.F.V. .
Virologypathobiology anddiagnosis
OfC.S.F.V.
Techniques fordetecting antibodies
Eplzootiology ofswinefever
Immunology
StudiesonC.S.F.V. andi'relatedv.iruss
ReviewsonTogaviruses .
Ultrastructure ofswinefevervirus
Serological relationship betweenswine
fevervirusandotheragents.
Incidence ofantibodytoswinefever
virus1ntheU.K.
Viralcontamination offoetalbovine
serum.

-3-
SUBJECT
«INSTITUTETITLEOFTHEPROJECT
V• ' •' .
TheanimalVirusresearchInstitute-
PIRBRIGHT (U.K.)-Studiesonthecomposition ofC.S.F.V.
Department ofAnimalHusbandry,University
ofBristol (U.K.) --Immunesystemofthepig
African SwineFeverVirus
'ΛCentroRegionaldeInvestigación yDesarollo
Agrario,Departmento Virología Animal-
MADRID(Spain),-Studiesonthecomposition ofA.S.F.V.
-Changesinthebasicbiological
properties oftheAfricanswine
feverfieldafvirus.
1TheanimalVirusresearchInstitute -
PIRBRIGHT (U.K.)-Studies onASFV
-AssayofASFV.
-Natureofresistance toASFV
-Airbonetransmission ofthevirus
ν· · Laboratorio Nacional deInvestigado
Veterinaria -LISBOA(Portugal )-Studiesontheproperties ofASFV
-Pathogenesis
Laboratoire CentraldeRecherches Vétérinaires
-MAISONS ALFORT(France)-Studies ontheVirus
-Diagnosis ^
-Pathogenesis andimmunobiology
* ;
»
• - v ' iStateVeterinary InstituteforVirusResearch-
LINDHOLM-KALVEHAVE (Denmark)
CentroRegionaldeInvestigación yDesarollo
Agrario,Departamento Virología Animal-
MADRID(Spain) ,:Iι .·!-Crossedimmunoelectrcphoretic
|characterization ofvirusantigens
}incellsinfectedwithASFV
I '
1 '.:''

ANNEXII
■ListofSemingrs andWorkshops organized forthepurpcsecf
co-ordination ,fatheClassical andAfricanSwinefever'programm e
I.SEMINARS ■\. '.'
• *
•Seminaron"Porcine Immunology "
-Thiverval-Grignon ,September 17-18,1973*
*"'V –I I'
2.Seminaron"StudiesonVirusReplication ".
Brussels ,May2-4»1974» ;
I
3.Seminaron"Diagnosis andEpizootiology orClassical Swinefever",
Amsterdam ,April30–May2,1975* '-
4.FAO/EECExpertConsultation ontheEradication ofAfricanandClassical
.swinefevers, ■
Hannöver,September 6-10,1976 ,
I.WORKSHOPS
1.Workshop on"Virological Methods",
Utrocht,September2-6,1974*
**•» '' *■- 1 '
2.Workshop 6n"Immunological Technique ",,
Lindholm,June9-14•1975*
3*Workshop on"Diagnosis ofAfrican SwineFever",
Madrid,February 24–28,1975»
4.Workshop on."Diagnosis ofClassical SwineFever",
Lelystad,May1»1975- V
5.Workshop on"Concentration andpurification ofHogCholeraVirus",,.
■Perugia,March'2**14,1975-'
6«Workshop on"Purification andConcentration ofAfricanSuinefevervirua"
Madrid,September 18-26,1975*

ANNEXIII '
•LISTOFPUBLICATIONS RESULTING FROMTHERESEARCH
PITSWITISFEVERVIRUSES '
Thislistofreferences doesnotincludethepaperypresented inthe
FAO/ESCconsultation onEradication pfClassical andAfricanswinefevers
HAEK0V3R.6-10September 1976.
BTTDCSF0R3CIFJÏÏGSA2Î3TALT FÎ)RVTRU3!CRA!ïïCin:iTE7T 1ERTIERS-TuBTTrCfTSIT
KORIT,G.'tfberdieVeränderung vonKrankheitsverlaufaforraendor
Schweinespe3tunddamitverbundene Virulenzverfir>derun.j;on
desVirxianachStimulierung der"Lymphozyten ".
-Berl.MUnch.TierKrztl .Wschr.86.447-449(1973)
KOMJ,C..MATTHA2U3 ,W.fLORSIIZ,R.andJAKUBIK,J.
UbereineGlobulin (Transferrin )undAntikb'rperbildunfjs -
störungwährenddesIieukopeniestadiums derSchweine-•
pesterkrankung .
Z.Immun.-Forsch.Bd,145.S-?139-155(1973)
KCRÎT,G..undLORETTZ,R.J.
Blutbilduntersuchungen beiverschiedenen Verlaufcforaen
derSchweinepestinfektion unthrbesonderer BorttcksichtL-
'gungvontymphozyttlrcn Reaktionen Zellschatten ,B<n/eg.m^;j
formenvonLymphozyten ("Lycozyten")undKnochenmark-
reaktionen '
-Zbl.Bakt.tyg.,I.Abt.Ofig.A229.229-322(1974)
* *
HATTHAEU3 ,W.undKORÏÏ,G. '
UberdieAuftrennung vonKristallviolettvakzinen cegendie
europal*sche Schweinepest zurIsolierung undCharakter Lcie-
rungdesImmunosierenden Agens.
-Archivfürdiegesamtevirusforschung ,4£,133-144(1974
XATTHAEU3 ,W.undKCRÏÏ.G. ;t
Ua3Verhalten vonpräzipitierenden Substanzen ansnomalen
OrganenundZellenimVergleich zudemderpräzipitierenden
Pankreas&anti genemitSchweinepest infizierte Schweina
-Zbl.Vet.Med.B,22,239-253(1975)

ÎIATTHAEU3 .W.uniKORïI,G.'-i \^
Serologische Identifizierung undVerhalten doshoch­
•(virulenten Schweinepestvirus inderImmunoelectrophornse
-Zbl.Vet.Ked.B.22,47-59(1975)'•.
K0R2Î.G.und:.!ATTHAEU3 ,M.
Krankheit s-,ImmunvorgStnge undvirulenzveründerun^en 'nei
!derSchweinepest aleReaktionen desbetroffenen I^jepho-
tqyeloiden Systems :, *,
-Berl.KUnch.-Tierärztlr Wschr.83.6-9(1975)'I
MATTHAEÜ3 ,.W.rKCRN,G.undSCHLOTTERER ,E.
•Imraunoelectrophoretischo StUiienUberCharactcr und-Cpe2ifi >
t8tderImmunpräzipitate vonSchweineseren undZellsucPen­
sionennachderInfektion mithochvirulentem Virusder
europäischen Schweinepest
Zbt.Ved.Med.B22.inpresa(1976)
MATTHAEU3 ,W.
KORtf,0.Isolation andcharacterisation oftheimmunising agentof
crystalvioletvaccines against HogCholera
Anenhancing effectontheprocessofswinefoverdiueanc
andthevirulence bystimulating lymphocytes
C.E.C. ,Seminaron"PorcineImmunology "Thiverval-Crignon
17th-l8th September 1973(EUR5450)
MATTHAEU3 .W.On^the'specificity ofantigensofHofCholeravirusdetected
bytheagqrgeldiffusiontestandImmunofluorescence .
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individual swineandinvestigations onthedevelopment
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C„E,C.Seminas ?on"Diagnosis andEpizootiology ofClassical
Swiss©Pever"Amsterdam ,30thApril–2ndMay1975(EUR5486)
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Hifj.neP««/®rwAmsterdam ,30thApril-2ndMsy1975(EUR5486)
M0EMXG,WoThepurification ofviralglycoproteins byaffinity chroma­
tography onConASepharose
C.E„C.Laboratory manualforresearch onClassical and
AfricanSwin®Fever.EUR548?,52-55(1975)
ID;.IN3TITUT HA7T0FAL BERECI1ERCHES VETERINAIRES-UCCLE
CHARL3ER ,0„,LEUTTEN,J.,KELLEKAIJS ,G.,S7RCBBE,R.
Deelectroforetische mobiliteit vanVarkenspestvirus <yn
Runderdiarheevirus
LEUKEÏÏ,J.Vaccination contre ,1aPesteporcineetporteurdevirus
Arxn,Ked.'Vet.119,93-103(1975)

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CHARLIER »G.,STR03BB ,R.,LEUOT ,Je
Kwantitatieve Analyse vaj?öqProteïnen van normale
varkensoera
Vlaams Djerg .Tijdsch .43*249-252 (1974 )
;
Theimmunologically related swine fever anamucosal
disease precipitinogens andnucleotide metabolism
Vlaaras Dierg .Tijdeeh . 342-354 (1975 )
Purification andsome properties ofswine fever relatud
mucosal disease precipitinogen
Vlaams Pierg .Tijdaeh .£JL,339-348 (1975 )
Purification ofth©bovine viral diarrhoea /HogCholera
antigen
C,,E.C. Seminar on"Porcine Immunology "Thiverval Grignon ,
I?th-l8th September 1973 (EUR5450 )
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LEmm ,j..FLQRKKT ,A.
Diagnosis andepicootiology ofClassical Swine Fever in
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swine fever "Amsterdam ,,30th April -2ndMay1975 (EUR5486 )VAIIAEïïT ,A.
YAN AERT .4.
VM AERT .J.
IV.STATE VSTER XKARY INSTITUTE FOR VIRTJS RESEARCH -LI73THOTJM
ASKA ,J.,BASSE ,A.,OVER BT.E.
Pathogenicity for thepigSetusoftwoGerman Strains
ofHogCholera
Ford .Vet .Med .to'bepublished (1977 )
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Vaccination ofpigs against KogCholera (Classical swine
pever )with adetergent split vaccine
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Klassiksvinepest-ataktveltproblemforfaelles:::a.rkeds
landene
Særtryk Med.Danskefyrlaeg,56,553-555(1973)
G'SERBYnE«a»dSCEJERNIUG-THIESEN ,K.
Afrikansk svinepest (AS?)ogdenoudbredelse éEuropa
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2nd-4thMay1974(EUR5451)
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Diagnostic procedures inDenmarkincasecfsuspected
classical swinefever
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swinefever"Amsterdam ,30thApril-2ndKay1975(SUR548C
DAL3GAARD ,K.Co-Author in:
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analysisofvirions"tobepublished ,1977(S*TR5C2C)
LABORATOIRE CENTRAL DEnECIÎT^CHSS VETERINAIRES -MAISONS ALEOT
ATNAUD,J.K.,LARENAUDIE ,3.
Diagnosis ofsubclinical oratypical formsofswinefever

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C.E.C,Seminaron"Diagnosis andEpi?.ootiolo»y ofClassical
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Ch&r&nerisation ofthehumoralimmuneresponseinth«j N
piglet.
3èmeI.P.V.S.tyon,1$,1,juillet1975.
RCUZE,P.,HOÜDAYER,M.,etKETZCER,J.J.
Immunological responseinpigletstEffectofpaaciv-i !epeci
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Influence del'immunité passivetransmise parlecolositrura
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Diagnosis ofsubclinical oratypical formsofBvinefever.
Demonstration oflowvirulent strainsintissueculture and
detection ofneutralising antibodies .
Am?JD.J.K.Characteristics ofanewlitfeVirusvaccinaagainstswine
feverpreparedintiepviacultureatlcwteoperature
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BHOHFpP.Jc Theimnmnesystemoftheyoungpig
CECSsminaron"Porcine Imraunology"TMverval-Grig -non,
17th»l8th September 1973(EUR54-50)
F.J,£01*153 Antigenic stiasu3e.tioainthepig
CkCSaœiniTen. onVirusrapiiee.tio »jjîrv/îfïeXef
&!<?-* X974(Eï?R

v)T»IBSTÍTOTO SUC70í;>.L VBimSSTTOACTOSSS AGRt&JÀS ■-'N^n *
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SAK^SWWEW'A «CUBAS..A.„8GZAKA,A»eT,,SEE51A,'J.M-,
©L5aS«.?«>„SåBfJLISOg Ha«S««a*AZAZ,S*-
^Hodifica'sioa efeosi*®aiapropeytias eatfc©African Swiss©
••*F©verVtr-xa* •''
Presaats-io «1Seai»s*i3 da"Stuáies ontírusBoplioatloa ".
Bielas,t~4M^fo .1$74<1976)-(El®5451)o.
SOUXfc,JL wTh«dissaoala ofClassical Swia©Fsw in&pain*.
Pressatalo «1Searflaarl©sabre«Diagnosi »ftisdSpi^«tiQlogy
©fClassioal -Swia®,F®v®rw,'AMStwia»,30ÀVrìl«»2'May©
1975(El®5486)..•^;
0BB¿3«A<,«aiSAECHEZ-BQTIJA C.
"Laboratory DiagaosisofClassical S'WinsFevariaSpain".
Preent&doelSeaaiaario deMF0A«G»/E.E,,G,'Expert.Consul–
tatioaontheEr&dio&tioa ofAfricansadClassical Swina
F®varwHsanover.6–10d«Saptieabra d©1976(SUR54&7)
©RM3g&„aMSAHCHEZ-BQTIJA,C.
"Differential Diagnosis Ue^weeaAfrican SwineFeyera?yi.
Aujassky'Diaeas© inLetaeoe^te Cultures *9.
Preseatsdo alSemiaario daWF.A„Q0/S.E.C.Expert,Consults--
timoatheEradioatl&a ofAfricaa aadClassical SvrisM
Fever".Haaaov®r,6*»10d®Septierabra ia1976(EUR5487)
QïffiASgA.-$ad8ABCHEZ-B0TIJA,■C.h-
"ifricaa Swia©Fever.IF.apidDiagnosis "byIdentificatioa
ofAntibodies EztrateA roatissuesusingindirect immuno­
fluorescence .II.Dete;tioaofchronicInfectioa aad
* -
carrierPigsPiga "h^/mmunoelectrooosphoreais®' ,
Pr&sentado alSent~>ario"F.A.O./E.E.C.ExpertConsultation
ontheEradicate a-pAfricaaaadClassical Swia©Fever 1*.
Hannover,' 6-10Septiembr© 1976(EDR5487).
:Μ?' ': 'χ;λ"'■■'/;''■■'■··''·"'·■■■·..'"V
TABARES,£«,RUIZ00H2ALV0,#.f.MARCOTECUI ,*.A.aadORDAS,A."
^Growth,ixrifioatioa aadFractionation ofAfricaaSwine
FeverVirus **
..Preservado alSeBinário **F.A.0./S.E.C.ExpertÇoasultatioa
..ΓΛ■■ ■
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Pyeee&t&xio elg'.ruairfc&Pio "P.A.Oe/E.E.C,ExpertCop.su!a-fcion
ontheEy&diestian efAfricanandClassical Svri.n$Fov^r",
Easnoyer,6-10S'sptieEfer *d®197^(EUR^8?),
SASCHEZ-BOTIJA ,C.,TABARES,P.,CRM,A.,30USA,k.,ECTXZCOHZALVO ,?.,
MARCQfEOTJ ,KeA»:co-authore .
StudiesonAfrican SvriaeFsverVirusjpurification and
analysisofth®virions.to"b®p«felieh«d (EGR^£Z6)
1711.LABORATORIO F«CXONAL B5IF7B3TIGACA0 VETERINARIA «LISBOA
KOÜEAEtTHESjJ.F.,eaüHES-PETISC A,J.L.
Replicação dovirujadapesteBUÍNAafricana (PSA;
"inrivo"gangli©Linfático .
XeReuniãoaooalàaSociedade Portts^uesa daMicroscopia
Electronica -12-13Eezembro 1975.

ANNEXIV OBJECTIVES OFTHEPPOGRAMME onClassical a^dAfricanSwinefe
Basicresearch
Diagnosisι:<
y
Research onCSPVJ
ΚVirusstructure
Virusreplication
Virusantigens
Virusgenstics
\Research onASFY
Research onpigitmunologv
Research onvaccination
/
Research onepidenialogy
Research oncontrolmeasuresT*7IfCK\Ù<àû
f
4iraur.o'ioqical resDcnse Inpics
Antivirasantibcdi 2S
Farcia !ir.TauniZ5t.icrj
Csvelopmientofvaccinas
Research onvaccination techniques
MaturaispreedofvirusA'J^lfcâfVtH
i1'
1Development ofepidemics
|Ercdlcaticn rethods
Immjmzaticn
«
Development oftechniques farearlydetection ofviruses
tducation offieldworkers {veterinarians )
Centrallaboratory andreference center •
FieldworksCo:1tactwl*thdiagnosticlaboratories
(Education offanners

ANNE' V(V•!ff'SULTSoftheClassical andAfricanSw'neFeverprcars^ae
VÎRUS
a^ntorphology • *
l>.Purification ofvirions
€.Analysisofviralprotein
«LSucleicacid
VIRUSSTRAINS
?i^io*geri)c *.
Apatnogenic
Vaccinestrains ••
Laboratory strains
VIRAI6ENETICS :
;tî'mutants,.'
3.Proteíns
b.Mucleicacfds
c.Mâmbranes
VIRALANTIGENS
Rembrane
Structural
Nonstructural
VIRALDIAGNOSIS
_Diagnostic technique
rViraiantigensViraiantl5©d1«s
ßadioimmunoassays
Inysi/m'zation
â.MaturôV "vaccination
', ■. /'
b.EffectonT48
lymphoblasts
Effectonthymus -'
c.Transmuiion
',Svadicat-iOTY
IsvnunfiatienVK3GCKOLtftA'V
•E.H.onHCi'id(JvOavailable
Achieved
Furthercharacterization isneeded .
RMA(N.W.3*10°)characterized«-rwASF7
^Available '' ^y*
'■Achieve!■""
Available "" *V
CNA{H.W.100x10°)partially V^;
.characterized ,■Information on.
internalorganizatio.i(by restriction
.enzyews)needed. »,
Thereasonfordifferences in...
•-pathogenesis notknown.v:.»
Isolatedandavailable --t,vNv/■
•"Hotavailable ^
Requiredforthestudyofviralgenefunctions •Requiredfortlistudyofviralgene
-... ^>fonctions ^^'Isolated anddescribed
*■\
Analysesofinfectedcallsarerequired „:Analysesofcellassociated proteins
^ ;andtheirbicsynthesfs .arerequired
*»- -•. -»ïv-+*
ComnonAgwithBVD
Glycoproteins .
ProteinsfurWeranalyses. flita»
•arerequiredAnalysesare;required;>'■£
•Ν'",,·'-ν.·- .··.".*
BVDandHCcommonAgswstb^,isolated ,VViralAgmustbeIsolatedand.■'--v -characterized U.
:v"Neutralization testsneedeq Neutralizing antibodies -r.;-■
\:V-/'-v.? %Comparisonofvirus'isolatesis
■V- .•;Vrequired \";v
Motavailablean4furtherresearchneeded ^RIAisrequired r?'■
Riseofantibodies ;protection bylive
,anddeadvaccinesisfcno*nNotavailable .JIBIvaph,studiesareneeded
•Interaction betweenlymphoidceils *' •'
EffectonOwmost " -iirbw^infectior.weilMtlw,
.fmmiz&Cion &'control -iRèasureî »
arenec-dcdEradication prooranneeded

ANNEXVI
FINANCIAL STATEMENTfortheresearchprogrammeonClassicalandAfrican
SwineFever ■
inUA
:Fundsavaila-:Fundsused:-.t-,-- •runu>dVdiid"•rwi<u» ••/INSTITUTES b,1nUA.(UA):4u3ed
Hanovre •60»328s .60.200i~100
Tübingen N.560.656 *t43.531-t72
Uccle :257.520\■î,'174.604v~.:68
Alfort :79.224ï71.418v?90
Thiverval-Grignon v:225.053 .:216.413 ■:96
Brescia î100.454 :87.588r-:87
Perugia :91.360:90.560:99
Teramo ï65.900,,:57.201 -!87
Lelystad :110.995 :85.695 :77
Utrecht :35.415 :24.894 .:70
Madrid :247.999 :247.277 .s-100
Lisbonne s400.000 231.949 ï58
Dublin î102,930 :-102.930 :100
Pirbright :302.203 :131.276 >:43
Lindholm :132.000 :131.931 s-100
Bristol î109.095 :105.165t:'96
Weyhidge s69.370 s48.924-ïV71
:Coordinationi•
-»272.500 236.672 î87:
:TOTAL 2.723.003•*2.148.228 s'7\9Î