ARCHIVESHISTORIQUES
DELACOMMISSION
COLLECTION RELIEEDES
DOCUMENTS "COM"
COM(76)397
Vol.1976/0119

Disclaimer
Conformément aurèglement(CEE,Euratom)n°354/83duConseildu1erfévrier1983
concernantl'ouvertureaupublicdesarchiveshistoriquesdelaCommunauté économique
européenneetdelaCommunautéeuropéennedel'énergieatomique(JOL43du15.2.1983,
p.1),telquemodifiéparlerèglement(CE,Euratom)n°1700/2003du22septembre2003
(JOL243du27.9.2003,p.1),cedossierestouvertaupublic.Lecaséchéant,lesdocuments
classifiésprésentsdanscedossierontétédéclassifiésconformément àl'article5dudit
règlement.
InaccordancewithCouncilRegulation(EEC,Euratom)No354/83of1February1983
concerningtheopeningtothepublicofthehistoricalarchivesoftheEuropeanEconomic
CommunityandtheEuropeanAtomicEnergyCommunity(OJL43,15.2.1983,p.1),as
amendedbyRegulation(EC,Euratom)No1700/2003of22September2003(OJL243,
27.9.2003,p.1),thisfileisopentothepublic.Wherenecessary,classifieddocumentsinthis
filehavebeendeclassified inconformitywithArticle5oftheaforementioned regulation.
InÜbereinstimmung mitderVerordnung(EWG,Euratom)Nr.354/83desRatesvom1.
Februar1983überdieFreigabederhistorischen ArchivederEuropäischen
Wirtschaftsgemeinschaft undderEuropäischen Atomgemeinschaft (ABI.L43vom15.2.1983,
S.1),geändertdurchdieVerordnung(EG,Euratom)Nr.1700/2003vom22.September2003
(ABI.L243vom27.9.2003,S.1),istdieseDateiderÖffentlichkeitzugänglich.Soweit
erforderlich,wurdendieVerschlusssachen indieserDateiinÜbereinstimmung mitArtikel5
dergenanntenVerordnungfreigegeben.

COMMISSION OFTHEEUROPEAN COMMUNITIES
C0M(76)397final
i
Brussels-23July1976
Proposalfora
'''COUNCIL_DIRECTIVE
intheexamination fortrichinae (trichinella spiralis)
uponimportation fromthirdcountriesoffreshmeat
dirivedfromdomesticswine
(submittedtotheCouncilbytheCommission )\
/
\
'/
ι
C0M(76)397final

EXPLANATORY MEMORANDUM
ThisproposalforaDirectivefollowsuponthetaskentrustedtotheCommission
"bytheCouncilunderArticle21oftheDirectiveof12December1972on
healthandveterinaryinspectionproblemsuponimportation ofbovineanimals
andswineandfreshmeatfromthirdcountries(l).InthatArticleitis
stipulatedthat"Themethodandproceduresrequiredfordetectingthepresence
oftrichinaeinfreshmeatofswineshallbelaiddownbytheCouncil,acting
onaproposalfromtheCommission."
Thedrawingupofuniformstandardsinthisrespectalsoanswersarealneed
inconnectionwiththeCommunityhygienearrangements introduced bythe
abovo-mentioned Directiveinrespectofimportofpigmeatfromthirdcountries.
Thesearrangements cannotbetotallyeffectiveunlesstheycoverIheproblem
ofdetectingtrichinae.
Thetextproposed,afterestablishing theprincipleofsystematicexamination
ofallswinewhosemeatisintendedfortheCommunity,laysdowntheconditions
underwhichtheexamination mustbecarriedoutanddescribesthemethods
whichmustbeused.Thetestalsoprovidesthat,incertainspecialcasesand
Undercertainconditions,theexamination maybereplacedbytreatmentof
themeatrecognizedaseffective,toensurethedestruction ofanytrichinae
whichmightbepresent. j
I
!
(1)O.J.n°L302,31.12.1972,p.28

Proposalfora
CouncilDirective
ontheexaminationfortrichinae(trichinellaspiralis)
uponimportation fromthirdcountriesoffresh
meatderivedfromdomesticswine
THECOUNCILOPTHEEUROPEANCOMMUNITIES ,
HavingregardtotheTreatyestablishing theEuropeanEconomicCommunity;
Havingregard,toCouncilDirectiveNo72/462/EEC(l)of12December1972,on
healthandveterinaryinspection problemsuponimportation ofbovineanimals
andswineandfreshmeatfromthirdcountries,aslastamendedbyDirective
No75/379^EEC(2)andinparticularArticle21thereof;
HavingregardtotheproposalfromtheCommission ;
WhereasinthesaidDirectiveof12December1972theCouncilprovidedin
Article21forthelayingdownofthemethodandproceduresrequiredfor
detectingthepresenceoftrichinaeinfreshpigmeat;
Whereastheapplication oftheabovementioned Directivewillnothavethe
desiredeffectsaslongasdisparitiesexistbetweentheMemberStatesas
totheguaranteesrequiredinrespectofthedetectionoftrichinaeupon
importation offreshmeatfromthirdcountries ;whereasitistherefore
necessary,inordertoremovethesedisparities ,tolaydownCommunity
arrangements inthisfield;
Whereas,inordertoprotectconsumerhealth,itisnecessarythatfresh
pigmeatbesystematically subjectedtoanexamination bymethodsrecognised
8Seffective,inordertoeliminatesamplescontainingtrichinae ;
Whereaswhentheexaminationiscarriedoutintheexportingthirdcountry,
itmustbecarriedoutinslaughterhouses whichcomplywithcertain
conditionsandwhichcontain,inparticular,ascreeninglaboratoryprovided
withsuitableequipment;
Whereasinordertobeabletodistinguish themeatsamplesexaminedfrom
thosenotexamineditisnecessarytoprovidefortheaffixingofaspecial
marktomeatsampleswhichhavebeenexaminedwithanegativeresult;
Whereasthereshouldbeaprocedureestablishing closeondeffective
cooperation betweentheCommission andtheMemberStatesforassessingthe
advisability ofpermittingestablishments inthethirdcountriestocarry
outthisexamination ortoworkonthemeatexaminedendalsoforbringing
thetechnicalprovisionsrelatinginparticulartotheexamination methods,
therequirements concerningthescreeninglaboratories andtheprocedurefor
markingexaminedmeatintolinewithtechnicalprogressandwithexperience
acquired;
(1)OJL302,31.12.1972,p.28.
(2)OJL172,3.7.75,P.17.

tvn^reaa itisexpedient toprovide for the possibility ofdispensing wifch .:-n
ex ;..minat ion incertain special cases ana replacing itbyatreatment ofthe
meat '■■}•iensures thedestruction ofany trichinae which might bepresent ;
whereas this treatment must nevertheless becarried out according to
certain well defined procedures and inestablishments fulfilling certain
conditions ;whereas there should beaCommunity procedure fordetermining
thecases andtheconditions inwhich theexamination maybedispensed with ,

HAS ADOPTED THIS DIRECTIVE :
Article 1
Forthepurposes ofthis Directive :
(a)"Fresh meat "means meat ofdomestic swine asdefined inArticle 2(o)of
,theCouncil Directive No72/462/EEC,
(b)"Examination "irefers totheexamination fortrichinae .
Article 2
1.Theimported fresh meat must ,ifitcontains skeletal muscles (striated
muscles ),have been examined systematically fortrichinae under the
supervision and ontheresponsibility ofanofficial veterinarian .
2.This examination must becarried out inaccordance with one oftie
methods set out inAnnex I.
3.The examination normaly must take place inslaughterhouses approved in
accordance with Article 4oftheCouncil Directive n°72/462/EiiCand
authorised tocarry outthis .examination inaccordance with theprovisions
ofArticle 3.
ITheexamination shall becarried outbefore thehealth marking provided
jforinChapter XofAnnex Btotheabovementioned Directive .
Incases where theexamination hasnotbeen carried out inaslaughter­
house situated inthe exporting third country ,itshall bemade atthe
time ofthehealth inspection provided forinArticle 24(2 )oftheabove -
mentioned Council Directive and atthe inspection posts referred toin
Article 27(l)(b)ofthat Directive .
4.(a)Meat which hasbeen examined with negative results shall bemarked
inaccordance with Annex III .Iftheexamination takes place inthe
slaughterhouse this marking must bemade before thehealth marking
i provided forinChapter XoftheCouncil Directive n°72/462/EEC.
(b)When marking with ink ,colouring mayonly beused asagreed in
accordance with provisions provided forinArticle 17(3 )ofthe
i Directive referred toinpoint 4(a)above .

-4-
?Article 3
1,(a)Theauthorization ofaslaughterhouse tocarry outtheexamination
andofacutting plant tocutuporbone meat which hasundergone
such examination shall hedecided oninaccordance with the
procedure laid down inArticle 9.Inparticular aocount shall "betaken
of theguarantees given inrespect ofcompliance with theprovisions
ofthis Directive and inthe case ofslaughterhouses :
(a)thepresence oftherooms andapparatus necessary forcarrying
out theexamination ;
(b)thequalification ofthepersonnel responsible formaking the
examination ;
(b)Aslaughterhouse orcutting plant shall beapproved only ifthe
competent authorities ofthethird country have officially recognised
that theslaughterhouse orcutting plant isinaposition tosatisfy
theconditions laid down inArticle 4and inAnnex III ;also ,inthe
case ofaslaughterhouse ,that ithasalaboratory which complies with
theconditions laid down inAnnex II,Chapter Iand isinaposition
tosatisfy ■ftierequirements oftheother Chapters ofAnnex IIandthose
ofAnnex I.
2.Onthelist orlists referred toinArticle 4(4)oftheCouncil Directive
; n°72/462/EEC ,aspecial indication shall beinserted against the
names ofthe slaughterhouses andcutting plants approved within the
meaning ofparagraph 1above .
Article 4
1.Inslaughterhouses authorised tocarry out1heexamination inaccordance
with Article 3tswine the meat ofwhich isintended foraMember State
oftheCommunity must beslaughtered indifferent rooms oratdifferent
times than the swine the meat ofwhich isnot intended for aMember State
oftheCommunity ,unless themeat ofsuch swine isexamined inaccordance
with the same procedure .
2.Thecutting andboning ofmeat which wasexamined inaccordance with
paragraphs 3and 4ofArticle 2andwhich isintended foraMember State
oftheCommunity must becarried out incutting plants asr«ferred toin
paragraph l(b)ofArticle 18oftheCouncil Directive n°7?/462/EECand
authorized for "ftiis purpose inaccordance with theprovisions ofArticle 3«
Inthese cutting plants thecutting andboning ofthe said meat shall be
carried out indifferent rooms oratdifferent times than meat not
intended foraMember State oftheCommunity ,unless thelatter hasalso
been examined inaccordance with the same procedure .
Article 5
Theinspections inthird countries provided forinArticle 5 "t*1®Council
Directive n072/462/ECmust also verify whether theprovisions ofthis
Directive arebeing applied .

Article 6
1.Atthe request ofthe importer orhisauthorized agend ,examination
shall not "berequired ifthemeat ha3 ,onitsarrival intheCommunity ,
ontheresponsibility ofandunder thesupervision ofthe official
veterinarian ,undergone heat treatment inaccordance with theprovisions
ofAnnex IV,point II.
2#Inaccordance with theprocedure laid down inArticle 9 byway of
derogation from theprovisions ofArticle 2(l)itmaybedecided that ,
under certain conditions ,meat from certain third countries orparts
!ofsuch countries need not "beexamined but maybechilled orfrozen in
iaccordance with theprovisions ofAnnex IV,point I.
This treatment shall becarried out ;
:–either inanestablishment situated inthe exporting third country and
approved inaccordance with theprovisions ofArticle 4oftheCouncil
: Directive n°72/462/EEC ,
i-orataninspection post asreferred toinArticle 2(3)ofthis Directive .
Chilling orfreezing must bethe subject ofanattestation bytheofficial
veterinarian on "OBhealth certificatea mentioned inArticle 22paragraph 3,
andinArticle 25oftheCouncil Directive n°72/462/EEC ,accompanying the
meat .
Article 7
TheMember States shall draw upand communicate totheCommission the list
ofinspection posts asreferred toinArticle 2(3)atwhich :
-theexamination maybecarried out , .
-thechilling andfreezing referred toinArtiole 6(2)maybeoarried out.
They shall ensure that these posts have theinstallations necessary for
carrying outtheoperations inquestion .
! Article 8
Theprovisions of1heAnnexes ofthis Directive maybeamended orsupplemented
inaccordance with theprocedure laid down inArticle 9.
' Article 9
1.Whereas theprocedure laid down inthis Article istobefollowed ,the
matter shall without delay bereferred totheStanding Veterinary
Committee (hereinafter called the "Committee "),setupbytheCouncil
Deoision of15October 1968 ,byitsChairman ,either onhisowninitiative
orattherequest ofaMember State .

-6-
2.Within theCommittee thevotes oftheMember States shall beweighted
asprovided inArticle 148(2 )oftheTreaty .TheChairman shall not
vote .
3.TheCommission representative shall submit adraft ofthemeasures to
beadopted .TheCommittee shall deliver itsopinion onthemeasures
within atime limit setbytheChairman having regard totheurgency
ofthequestions under examination .Opinions shall bedelivered bya
majority offorty-one votes .
4.TheCommission shall sdopt the .measures andshall implement them
immediately ,where they areinaccordance with theOpinion ofthe
Committee .Where themeasures envisaged arenot inaccordrnce with
theOpinion oftheCommittee ,orifnoOpinion isdelivered ,the
Commission shall without delay submit totheCouncil aproposal onthe
measures tobetaken .
TheCouncil shall adopt themeasures byaqualified majority .
Ifthe Council hasnot adopted anymeasures within three months ofthe
date onwhich thematter isreferred toit,theCommission shall adopt
theproposed measures andshall implement them immediately .
Article 10
TheMember States shall bring into force the15th January 1979 atthelatest
the laws ,regulations andadministrative provisions needed inorder to
CQmply with this Directive .*TheCommission isbeing told atonce ofthis .
Article "11
This Directive isaddressed tathe Member States ..
Done atBrussels ,
Foi *the Council
The Président

ΓANNEXI
r.TSTHODSOFEXAMINATION FORTRICHINAE
Trichinoscopic examination
a)Apparatus
Anincandescent lamptrichinoscope with50xand.80-100xmagnification ,
Apressureglassconsistingoftwoglassplates-oneofwhichis
dividedintoequalfields-smallcurvedscissors,forceps,aknife
forcuttingspecimens,smallnumberedcontainersforstoringthe
specimensseparately,adroppingpipette,asmallglassofaceticacid
endasmallglassofpotassiumhydroxidesolutionforbrightening any
calcifications orsofteningdriedflesh.
b)Tnkingofspecimens
Inthecaseofwholecarcases,atleastonespecimenofthenizeofa
hazelnutistobetakenfrombothdiaphragmpillarsatthetransition
tothesinewypart.Ifthereisonlyonediaphragmpillar,one
specimenthesizeoftwohazelnutsistobetaken.'Intheabsence
ofbothdiaphragmpillars,twospecimensapproximately thesizeofa
hazelnutaretobetakenfromtheribpartorthebreastbonepartof
thediaphragmor,asthecasemaybe,fromthelingualmuscleorthe
jawmuscleorfromtheabdominalmuscles.
Forpiecesofmeat,fromeachpiecetakethreesamplesofskeletal
muscle,containinglittlefat,ifpossiblethesizeofahazelnut,
andtakenfromdifferentpoints,asfaraspossibleneartobonesor
tendons.
c)Method
Fromeachoftheabovementioned specimens,inthecaseofwholecarcases
andthepresenceofbothdiaphragmpillars,thetrichinaeobserver
cutssevenpieces,i.e.atotalof14,or,ifonlyonediaphragm
pillarispresent,14piecesthe'sizeofanoatkernel,fromdifferent
places,ifpossibleatthetransitiontothesinewyparts,and
compresses thembetweentheglassplatesinsuchawaythatnormal
printcanbeclearlyreadthroughtheslidepreparation .Ifthe
fleshofthespecimenstobeexaminedisdryandold,thepreparations
mustbesoftenedinamixtureofonepartpotassiumhydroxidesolution
toabouttwopartswaterfor10-20minutesbeforepressing.
If,inthecaseofwholecarcases,specimenshavetobetakenfromthe
ribpartorthebreastboneofthediaphragm,thetonguemuscleor
jawmuscleortheabdominalmuscles,then14piecesthesizeofonoat
kernelaretobecutoutofeachspecimen,i.e.atotalof28.
Fromeachofthesamplestakenfrompiecesofmeat,thetrichinosis
examinermustcut4fragmentsthesizeofanoatkernel,thatisto
sayatotalof12fragments.
Thetrichinoscopic examination mustbecarriedoutinsuchamanner
thateachpreparationisscannedslowlyandcarefully.Ifthe
trichinoscopic examinationrevealssuspectareas,thenatureof
whichcannotbedefinitelyascertained evenwiththestrong
magnification ofthetrichinoscope ,thesemustbecheckedby
microscope .

-2-
ANNEX I
Themicroscopic examination should becarried out insuch s.msmer
that each preparation isscanned slowly endcarefully ata
magnification of30xto40xatthemost .
Inthe case ofanuncertain result ,theexamination must "becontinued
onafurther number ofspecimens andslide preparations ,ifnecesscry
with theaidofhigher magnifications ,until complete certs inty is
obtained .Thetrichinoscopic examination must becarried out for at
least three minutes .
The trichinoscopic examination must becarried out for atleast six
minutes inthe case ofsubstitute specimens taken from the rib part
orbreastbone part ofthediaphragm pillars ,thelingual muscle or
the jawmuscle orthe abdominal muscles .
Theminimum time fixed for the examination does not include the time
necessary forthetaking ofsamples andmaking thepreparations .
Asageneral rule ,thetrichinoscopic examiner should not inspect
more than 840pieces aday ,though bywayofexception hemayinspect
upto1050 .
II,The artificial digestion method
a)Apparatus andmaterial
-knife fortaking specimens ,
-small numbered containers ,with closures ,forstoring samples ,
also forpossible repetition oftheexaminations ,
-incubator ,
-2-3litre glass funnel with stand ,aconnecting hose inrubber ,
clamps forfastening theconnecting hose ,
-aplastic sieve (approximately 18cmindiameter ,andapproximately
1mmmesh ),
-gauze ,
-asmall tapered tube with aclosed point ,
-aglass block dish ,
-ameat grinder ,
-astereo-microscope ,
-digestive liquid according tothe following composition :
10gofpepsin (1200 u/g),5nilHC1 (atleast 37%)made uptoa
litre with tapwater .
b)Taking ofspecimens
1.Inthecase ofwhole carcases take aspecimen ofatleast 20g
from adiaphragm pillar atthe transition tothe sinewy part .
Inthe absence ofdiaphragm pillars take aspecimen ofatleast
20g from the rib part orthebreastbone pa11ofthediaphragm
orfrom the lingual muscle orthe jawmuscle orthe abdominal
muscles .
2..Forpieces ofmeat ,take asample ofatleast 20gofskeletal
muscle ,containing little fatand asfar aspossible near to
bones ortendons .
c)Method
Fortheexamination ofacollective specimen from tenpigs ,a10g
sample isprepared from each individual 20gspecimen .Theremaining
10giskept foradditional single-specimen examination should this

-3-
ANNEXI
benecessary.Tenspecimens,eachweighing10g,erecombinedintoa
collectivespecimen;itisgrounddowninameatgrinder(discwith
2mmperforations )andspreadlooselyonasievecoveredwithalayer
ofgauze.Thesieveisthenplacedinafunnelconnectedby&length
ofrubberhosetoasmalltaperedtube,thepointofwhichisclosed;
thefunnelisfilledfromtheedgewithdigestionliquiduntilthe
testmaterialiscompletelycovered.Theproportionoftestmaterial
todigestionliquidmustbeapproximately 1;20to1:30,After18-20
hoursofincubationat37-39C,thesmalltaperedtubeisdisconnected
andremoved.
Aftercarefuldrawingoffofthesupernatantliquid,thesediment
presentinthetipofthetubeiscarefullywashedinablockdish.
Examineforthepresenceoftrichinaewithastereo-microscope at
20xto40xmagnification .
Inthecaseofapositiveordoubtfulresultfromtheexamination ofa
collectivespecimen,theremainingsinglespecimensshouldbeexamined
individuallyafteradditionofafurther20gf^romeachpig,orinthe
caseofpiecesofmeat,theadditionof20gtakenfromeachpiece,
accordingtob)above.
Ill,Methodusingtheartificialdigestionofcollective samples
a)Apparatuspjidreagents
-knifeandtweezersforcollectingspecimens,
-meatgrindertheholesofwhichshouldhaveadiameterbetween2-3mm,
-a3mlErlenmeyerflaskwitharubberorcotton-wool plug,
-aconicalseparationfunnelof2000micapacity,
-enordinaryA-ba.sestandofapprox.28cmlengthwithan80cmrod,
-aring,diameterapprox.10-11cmwhichcanbefixedtothestand,-aclampwithaflatvice(23x40mm)whichcanbeattachedtothe
standbymeansofadouble'coupling,-anEndecottsievenumber80(meshfineness177)withanexternal
diameterof11cmfittedwithbrassgauzeorstainlesssteel,
-aplasticfunnelwithaninternaldiameterofnotlessthen12cm,-sJiordinarydissectionmicroscope (magnification 40x)fittedwith
itsordinarylamp,oranordinarybinocularmicroscope (magnification
40x),
-atrichinoscope withhorizontaltableoi?forthecompressorium ,
-whenusingthetrichinoscope alarvaecountingbasinofthesame,
outershapeasthecompressorium withavolumeofapprox.60-65cm.
Thelarvaecountingbasinmaybeconstructed inthefollowingway:
Theshapeofthebasinconsistsofa23cmlongglassplate
beingofthesamethicknessasthatofasingleplateina
commoncompressorium .Thewidth,however,isslightlyless,
e.g.4.5cminordertoprovideforthefixationofaglass
plate,2mmthick,1.8cmhigh,and17.5eralong,onbothof
thelongsidesofthebottomplate.
Thebasinisclosedintheendsbyapplyingdirectlyonthe ■
bottomplatetwo5cmlong,1cmhigh,and2mmthickglass-
plate.Theheightofthebasin,mersuredfrominside,willthus
beabout1cm.

-4~
AMNEXI
Theplatesweregluedtogetherbyordinaryglassglue.Approx.
2.8cmofthebottomplatewc.sleftfreeatbothendsfor
protectionpurposes,andmakingconvenienthandlingofthefilled
basin.
Thevolumeofthebasinisapprox.60-65cm^intotal.
-whenusingthemicroscopeanumberof9cmdiameterPetridishesere
needed.
-aspeedmarker usedformarkingoutinthebottomofthePetridish
the1cmsquareformedexamination areas,
-anumberof10litrebinstobeusedwhenapplyingformoltreatment
oftheapparatusandtheremainingdigestivejuiceinpositivecases
offindings,-concentration (37$)hydrochloric acid,
-Merck30000unitspergrammepepsininpowderform,orpepsinwith
knownstrengthfromanotherfirm,
-oneortwotraysforcollectionof100samplesofapprox.2gofmeat.
b)Collecting :ofspecimens
1.Inthecaseofwholecarc.a.sestakeaspecimenofapprox.2gfrom
adiaphragmpillaratthetransitiontothesineivypp.rt.Inthe
absenceofdiaphragmpillarstakeaspecimenofthesamesizefrom
theribpartorthebreastboneportofthediaphragm,fromthe
lingualmuscleorthejawmuscle,ortheabdominalmuscles.
2.Forpiecesofmeat,takeasejnpleofapprox.2gofskeletalmuscle,
containinglittlefatandasfaraspossibleneartobonesor
tendons.
c)Mcthod
-Approx1gofsampleisdrawnfromeachof100samplesfromthepigs.
Thepooledsampleisgroundonceinthegrinder.
-Thegroundmeatisplacedinthe3litreErlenmeyerflasktogether
with^gpepsin,approximately 2litresoftapwater,temperatureabout37-40C,and25mlconcentrated hydrochloric acid.Themixtureis
shakenforsolutionofthepepsin.
ThepHinthesolutionwillbeabout1.5-2.
-FordigestiontheErlenmeyerflaskisincubatedat37°Cforapprox.
4hours.Theflaskisregularlyshakenduringthetimeofincubation
e.g.onceortwiceeveryhour.
-Thedigestedsolutionisfilteredthroughthesieveintotheconical
21.separationfunnelaxidleftundisturbed inthestandforatleast
1hour.
-Atotalvolumeofapproximately 45cra^isru*1°'lfcof"thefunneland
equallydividedbetweenthreePetridishes,thebottomsofwhichare
markedoutin1cmsquaresi.e.15mlineach.
-EachPetridishisminutelyexaminedforlarvaltrichinaeunderthe
microscope (around40xmagnification ).
-Wherelarvalcountingbasinsareemployed,the45cm^aredistributed
betweentwolarvalcountingbasinsandexaminedwiththetrichino-
scope.

-5-
ΑΝΝΞΧI
Thelarvaeappearasourled-uporganismsinthedepositandlook
ratherlikewatchsprings.Theyareeasilyidentifiable andoften,
whenthewaterislukewarm,makerollingandunrollingmovementsof
the"spiral".
Ifeninsufficiently transparent sedimentforms,itisclarifiedby
meansofrinsing.Thenthefinalsampleof45mlispouredintoa
testtubeandallowedtosettleforfifteenminutes.Thesupernatant
isthencarefullysuckedoffandwhatremainssuspendedinabout45ml
oftapwater.
Afterafurthersettlingperiodoffifteenminutes,thesupernetant
isagaincarefullysuckedoffandthesedimentiscarefullyrinsed
withabout20mloftapwaterinapetridishendexamined.
Inthecaseofapositiveordoubtfulresultfromtheexamination of
acollectivespecimen,theremainingsinglespecimensshouldbe
examinedindividuallyafteradditionofafurther20gfromeachpig,
or,inthecaseofpiecesofmeattheadditionof20gtakenfromeach
piece,accordingtob)above.

1
ANNEXII
CHAFmRI
CONDITIONSFORTHEAUTHORISATION OFTRICHINAE(TRICHI1TBLLASPIRALIS)
LABORATORIES
1,Trichinaelaboratories must"besituatednexttotheswineslaughterhouses
andbeinpossessionofatleastthefollowing:
(a)aroomsuitablyequippedforthepreparationofspecimensandlockable
bykey;itswallsmustbesmoothandmustbecovered "byawashable,
light-coloured coatingorpaintuptoaheightoftwometres.A
preparation roommustbeprovidedforeachmethodofexamination used;
(b)anadequatelyequippedlockableexamination roomformicroscopyend
trichinoscopy ;
(c)equipmentprovidingadequateventilation and,ifnecessary,air
conditioning equipmentwhichensuresthattheroomtemperaturedoesnotexceed+25C;
(d)adequatenaturalorartificiallightingwhichdoesnotaltercolours;
directsunlightmustbeavoided;
(e)inthepreparation room,adequateequipmentforcleansingand
disinfecting hands;
(f)equipmentfordarkeningtheexamination room;
(g)ifnecessary,refrigerators inwhichtostoremeatsejnples;
(h)awashroomforcleansinganddisinfecting examinationequipment
(e.g.containersforsamples,compressors ,knivesandscissors),
with
-afloorcoveringwhichiswatertight androt-proofandeasyto
cleananddisinfect,
-smoothwallswhich,uptoaheightofatleasttwometres,are
coveredbyawashable,andlight-coloured coatingorpaint;
(i)changingrooms,washbasinsandrestroomsandflushlavatories;
(j)washbasinswithhotandcoldrunningwaterofpotablequality,
providedwithcleansinganddisinfecting materialsanddisposable
towels;
(k)watertightnon-corrodible containers,withhermeticallysealedlids,
whicharesodesignedthatunauthorised removalofthecontentsis
prevented,forcollectingthesamplesafterexamination ;
(l)adequatesuppliesofhotandcoldwaterofpotablequality;
(m)equipmentforremovingwastewaterwhichfulfilstheconditionsfor
theauthorisation ofslaughterhouses ;
(n)properarrangementsforprotectionagainstpests(insects,rodents,
etc.)

-2-
AN1TEXII
CHAPTER .II■
REQUIRETIEFTTS APPLICABLE TOSTAFF,PREMISES ♦.EQUIPMENT AM)INSTRUMENTS IN"
TRICHINAI!LABORATORIES
2.Absolutecleanlinessisrequiredatalltimesoflaboratorystaff,
premises,equipmentandinstruments.
(a)Staffmustwearcleanworkingclothesandwashtheirhandsseveral
timesduringworkinghoursandaftereachbreak;
(b)noanimaimayentertrichinaelaboratories :
(e)equipmentandworkinginstrumentsmustbekeptcleanandingood
repair.Theymustbecarefullycleansedanddisinfected several
timesduringworkinghoursandattheendoftheworkingday.
3.Waterofpotablequalitymustbeusedforallpurposes.
4.Asregardshealth,stafftakingmeatsamplesforexamination mustcomply
withtherulesinAnnexB,ChapterIV,points11and12,totheCounoilDirectiven°72/462/EEC.
5.Themeatsamplesnecessaryfortheexamination mustbetakenimmediately
afterslaughterandexaminedwithoutdelayinthetrichinaelaboratory
oftheslaughterhouse .
Itisprohibitedtocarryoutexaminations awayfromtheslaughterhouse
inwhichtheanimalswereslaughtered i
6.Toavoidfatigueanditsconsequences ,inspectionstaffshouldbegiven
shortbreaksduringtheworkingday.
CHAPTERIII
REQUIRETTENTSITTRESPECTOFTRICHITTOSCOPBS
Theconstruction anddesignoftrichinoscopes mustmeetatleastthe
followingcriteria:
1.simpleoperation
2.highlightintensity
-accurateresultsmustbeobtainableeveninaroomwhichiscompletely
dark,-aprojectorbulbof100W(12V)mustbeusedasthelightsource.
3.adequatemagnification
-normalworkingmagnification :x50
-strcngmagnification formoredetailedexamination ofsuspect
specimens:x70tox100.
4.resolvingpower
-aclearsharppictureofwell-defined colourmustbeobtainableat
eachmagnification
5.switchmechanism
-eachchangeofmagnification mustbeaccompanied byautomatic
adjustmentofthebrightnessoftheimage
6.increaseofcontrast
-thecondensormustbeprovidedwithanirisdiaphragmenablingthe
contrasttobeincreasedfortheinspectionofdifficultobjects-theirisdiaphragmmustbeeasytooperate(e.g.controlleveron
theplatformofthetrichinoscope ).

-3-
AWEXII
7.Easyfocusing
-rapidfocusing "bymeansofanadjustingring
-precisefocusingbymeansofacontrollever
8.Regulationofthevoltage
-adjustmenttothebrightnessinaparticularcase
9.One-waymovementofthecompressor
-anautomaticblockingmechanismmustensurethatthecompressor
movesin.onlyonedirection,topreventunintentional displacement
10.Freeviewoftheprojectorscreen
11.Projectorscreen
-atleast54cmindiameter
-highreflectingcapability
-durable
-dismountable
-easytoclean.

miExin
MARKINGOPM1DATWHICHHASBOTgXAMIfflTO FPUTRICHIN/U3
Markingofthemeatmustbecarried,outundertheresponsibility ofthe
offici&lveterinarian .Forthispurpose,heshallkeepandmaintain:
theinstruments intendedformarkingwhichhemayhandovertothe
assistantstaffonlyatthetimeofmarkingtaidforthelengthoftime
requiredforthispurpose,
thesealsmentionedinparagraph 5.Thesesealsshallbegiventothe
assistantstaffatthetimewhentheymustbeusedandintherequired
number.
Themarkmustberoundwithadiameterof2.5cm.Thefollowing
information mustappearonthemarkinperfectlylegiblecharacters :
-towardsthecentrethecapitalletterTwithbars1cmlongand0.2cm
wide,
-undertheletterToneofthefollowingsetsofinitials:CEE,EEG,
EWG,Ej$ForEEC.Thelettersmustbe0.4cmshigh.
Carcasesmustbemarkedininkorhot-branded ontheinsideofthethighs,
inaccordancewithparagraph 2.
Headsmustbemarkedininkorhot-branded ,withamarkmeetingthe
requirements ofparagraph 2.Withtheexceptionofthoseexemptfrom
healthmarkingbyvirtueofparagraph43ofChapterXofAnnexBtothe
CouncilDirectiven°72/462/EEC,cutsobtainedincuttingplants
fromcarcasesmarkedinaccordancewiththerulesmust,wheretheybear
nostamp,bemarkedinaccordancewithparagraph2beforethehealthmark
isaffixed.
Thelabelprovidedforinthe2ndsubparagraph oftheabovementioned
paragraph43mustcomplywiththeconditionsofparagraph6below.
Markingmayalsobeeffectedbymeansofaroundtag.Thisteg,tobe
affixedtoeachcutortoeachcarcasemustnotbereusable,mustbe
madeofresistantmaterialsandmustmeetallhygienerequirements .
Thefollowinginformation mustappearonthestampsealsinperfectly
legiblecharacters s
s-towardsthecentrethecapitalletterT,
-undertheletterToneofthefollowingsetsofinitials:CEE,EEG,
EWG,E$F,orEEC.Thelettersmustbe0.2cmhigh.
Thelabelprovidedforinparagraph44ofChapterXofAnnexBtothe
Directivementionedinparagraph4abovemust,inedditiontothehealth
mark,bearaclearlylegiblemarkwhichisthereplicaofthemark
providedforinparagraph 2.

ANNEXIV
I,Tf-roFREEZINGOFMEAT
1.Beforeitis"broughtintotherefrigeration roomthemeatmustbepre-chilledthroughouttoatemperatureof0°to+2°C.Meatwhich
isbroughtinalreadyfrozenmust"bekeptinthiscondition.
2.Thetechnicalequipmentoftherefrigeration room,endthefeeding
ofthesupplyofmeattoit,must"besuchastoensurethetthe
temperaturereferredtoinNo6isreachedandmaintainedinall
partsoftheroomandofthemeat.
3.Insulatedpackagingshouldberemovedbeforefreezing,exceptfor
meatwhichhasalreadyreachedthroughoutthetemperature referred
toinNo6whenitisbroughtintotherefrigeration room.
4*Consignments intherefrigeration roommustbekeptseparately end
lockedup.
5.Thedateajidtimewheneachconsignmentisbroughtintothe
refrigeration roommustbemarkedonit.
6.Thetemperatureintherefrigeration roommustbeatleast-25°C.
Itshouldbemeasuredwithcalibratedthermo-electric instruments
andcontinuously recorded.Itmaynotbemeasureddirectlyinthe
coldairflow.Theinstruments mustbekeptundertheprotectionof
alock.Thegraphsmustincludetherelevantnumbersfromthemeat
inspectionregisteronimportation andthedateand
timeofthecommencement andcompletionoffreezing,andmustbe
retainedforoneyearaftercompilation .
7.Meatwithadiameterorthicknessofupto25cmmustbefrozenfor
atleast240consecutive hours,andmeatwithadiameterorthickness
ofmorethan25cmto50cmmustbefrozenforatleast480
consecutive hours.ThiBfreezingprocessmaynotbeappliedtomeat
whichhasalargerdiameteroristhicker.Thefreezingtimeis
calculatedfromthepointwhenthetemperaturereferredtoinNo6
isreachedinthefreezingroom.
II.HEATTREA.'MEN'T
1.Thepackaging,unlessitisheat-proof,shouldberemovedbefore
heating.Themeat,whichmustnotbetreatedinanyway,shouldbe
storedseparatelyandkeptsealeduntilheatingiscompleted.

-2- 1478/VI/76-E.
ANÎTEXIV
Thecentreof"themeatshouldbeheatedataminimumtemperature of
+00°Gforatleast10minutes.Thetemperatureforeachheating
operationshouldbemeasuredthermo-electrically withcalibrated
instrumentsatthethickest(oratacentral)partofthepackaging
andthereadingsobtainedshouldberecordedcontinuously .
Forimportcontrolpurposestheappropriate logbooknumbersandthe
dayandmonthofeachheatingoperationshouldbeappendedtothe
records-whichshouldbekeptonfileforoneyear.