ARCHIVESHISTORIQUES
DELACOMMISSION
COLLECTION RELIEEDES
DOCUMENTS "COM"
COM(73)2047
Vol.1973/0368

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COMMISSION OFTHEEURQEHAE ?COMMUNITIES
COLI(73)2047final
Brussels .10December 1973
PROPOSAL FORA
COUNCIL DECISION
amending theDecision of14May1973adopting afive-year research and
training programme oftheEuropean AtomicEnergyCommunity inthe
fieldof"biologyandhealthphysics
(submitted totheCouncil "bytheCommission )
COM(73)2047final

XI1/368/73-E
1.;NTT?CDTTCTON
During thediscussion intheCouncil ontheproposed raultiannual research
and trafn ?ngprogramme oftheEAEC inthefield of"Biology-Eeaith Physics ",
the Commission repreaentati vestated that '"inthe event ofenlargement of
theCommunity ,hisInstitution will submit proposals totheCouncil for
theprogramme adaptations which prove tobenecessary inthis sphere ".
Q3/1156/71(AT'056)~J.
TheCouncil Decision of21June 1971 ,which forms part oftheCommunity
patrimony ,concerned ajoint "Radiation Protection "programme involving
17335million u.a .for five years and 97employees ,and asupplementary
programme (Geimany ,Italy sNetherlands )devoted to'the "Application3 of
nuclear techniques toagricultural and medical research ",with abudget
of56l0million u.a<y and 10employees .
Because oftheCommunity 'senlargement ,anamendment tothis decision was
adopted bytheCouncil on1*tMay 1973 ?raising theceiling ofexpenditure
commitments forthejoint programme to18886million u.a .andleaving
theceiling ofthesupplementary programme unchanged .Atthat time asthe
participation oftheNewMember States wasautomatic ,theCommission had
>
proposed adapting the joint programme only .TheCommission hadhowever
announced that anadaptation ofthesupplementary programme would be
presented induecourse (CCM(72 )1505 of8November 1972 ),because its
preparation would need more time .
Theparticipation ofthenewMember States inthesupplementary programme
issubject tothe same conditions astheparticipation oftheSix .Itis
therefore optional ,anddepends upon theattractiveness ofthsCommission 's
proposals forthese countries and for theCommunity .With regard tothe
Member States oftheoriginal Community oftheSixwhich didnot take part
inthesupplementary programme ,they areinvited bytheCommission toview
thepresent adaptation proposal asareconsideration ofthe "Applications "
programme which could ,ifthey wish ,induce them tomodify their former
position .

REASONS
Because oftheimportance ofunclear techniques and methods ,formedical
andagricultural research ,aiidbscause itisofinterest ,from this
standpoint ,.toexploit thepotential created intheCommunity ,three
Member States -Germany ,,Italy and theNetherlands -decide Jin1971 to
take part inafive-year research programme onthe "Applications of ''
nuclear techniques toagricultural andmedical research ". "
Theenlargement oftheCommunity does not change Ssheimportance nor the
interest 'ofthis subject ,but : ;
-itmeans th&re ismore research under way onthenew Community 's
territory ,..
-itmeans there aremore specialized laboratories able tocontribute
tothe Community programme and tohelp achieve its objectives .
TheCommission possesses considerable information onthework being
carried outinthenewMember States ,onthesubjects andareas ofresearch
which are ®faparticular interest tothem ,and onthe ties which already
exist with laboratories inthe Six .Itisobvious that this cooperation
should beorganized inorder toensure optimum efficiency ,andsheuld be
guided towards objectives whose importance isacknowledged byall .
Itfollows that thesupplementary programme on"Applications "should be
adapted : !
-insuch aways astopermit the ,gradu&l integration ofcertain skills
,and facilities available inthenew Member States , .
-while ensuring awider coordination ofeffort ,particularly with regard
•tocooperation between laboratories ,
-without changing theframework oftheprogramme ,butemphasizing certain
areas whose priority importance isacknowledged .

XI1/368/73-E
BEoEARCH SUBJECTS
/
3.1 .Theprogramme adopted bytheCouncil on21June 1971 includes the
following main research subjects :
/erricultural research :
-thedevelopment ofmethods ofraldiogenetic control ofinsect
pests asaparticular aspect ofintegrated control ,
-thedevelopment and utilization ofthe plant cell culture technique ,
-certain aspects ofexperimental mutagenesis ,including itsapplica­
tion toimprovement ofprotein patterns and toinduction ofdisease
resistance ^aswell asthe study ofincompatibility phenomena .
-certain aspects offood irradiation ,
-thestudy ofmovements ,absorption ,translocation andaccumulation
ofions and molecules insoils and plants .
radical research :
-radio-immunological methods ofmeasuring the proteic and non-
protein constituents ofplasma , *
-the use ofshort-lived isotopes for theanalysis ofcoronary
circulation andkidney andlung functions ,and for thediagnosis
cfhypertension ,
-thedevelopment ofanon-traumatizing method for theanalysis of
cardiac kinetics ,
~inview offast neutron radiotherapy :calculation and measurement
ofabsorbed dose and neutron spectra inbiological matter .
Atrore detailed description of'this programme ,listing itsvarious
contracts ,isgiven inAnnex 1'. '

XI1/563/73-E
J;.2.Gntacts onatechnical level with institutions and laboratories
inthe new Kember otates have shown that collaboration with these
institutions could bedecisive fordeepening andcompleting the
work atpresent under way .Kention cculd bemade ofthe following
examples ,forwhich collaboration would undoubtedly beprofitable .
Agricultural researchî
-the culture ofplant cells ,and particularly themethodology for
obtaining haploids ,the induction ofembryoids inanther cultures ,
theproduction andsomatic fusion Ofprotoplasts (Annex 2),
-thestudy and utilization ofincompatibility phenomena ,particu­
larly sporophitic systems ,thegenetic structure ofincompatibi­
lity ,andtheproduction ofinterspecific hybrids (Annex 3)»
--thestudy ofphysical and chemical soil parameters ,ofmovements
ofions ,water andherbicides insoils ,offauna and flora acti­
vity insoils ,ofroot development ,inconnection with cultivation
techniques such asthe "reduced tillage "technique (Annex 4).
Hecli^g-l research !
-study ofvarious human physiopathologicai problems ,with theuse
ofnuclear techniques ,
-evaluation ofradiobiological andclinical aspects offast neutron
therapy (Annex 5)»
-dosimetry offast neutrons used inradiotherapy ,andcomparison
ofresults obtained invarious centres (Annex 6).
Thelist ofsubjects suitable formore thorough investigation by
collaboration with thelaboratories ofhitherto non-participating
countries isnotexhaustive .Forinstance ;itwould undoubtedly be
beneficial forall concerned iftheattempts nowunder way inthese
countries todevelop methods ofradiogenetic control could beincor­
porated inthepresent Community programme .

-6- XII/3&8/73-E
FU::DS AND STAFF
-The .Council Decision of1*fKay 1973 was aconfirmation ,asfarasthe
supplementary programme isconcerned ,oftheDecision of21June 1971 *
which granted 5610million u.a »over 5years ,and fixed astaff
ceiling of .10persons ,thus ensuring continuity ofthework carried
out inGermany,j Italy and theNetherlands until theendof1975 *
-After studying thescientific problems involved ,both inthehember
States already taking part intheprogramme and inthe others ,ithas
been possible tosingle out certain fields inwhich further study is
desirable and toeval'.iate the funds needed for this purpose .
*""-}eaverage participation cfthe Commission ,peryear and per project ,
is25000u.a.*,except incontracts inthe "Neutron therapy-Dosimetry
field where itamounts to12COO u.a .
Newprojects should beundertaken fora2-year period (197^-75 )inthe
new iiember States aswell asintheformer Member States .Moreover ,
certain programmes should besupplemented bystepping upthework
already under way intheoriginal participating States .Theincrease
inthefunds granted totheprogramme would amount to1.8million u.a »
for 2years ,entirely devoted to+27"Agriculture "projects ,grouped
intheprogramme ,and to+17"Medicine "projects .
Asanapproximate guide ,apossible distribution ofprojects could be
Agri or.1ture
New Member States 1h
Honparticipating former Member States 8
Participating fcrmer Member States 5
This figure isbased ontheparticipation oftheCommission inthe
groups ofcontracts "Cell culture ","Radioentomology ",inthesub­
contracts oftheEURATOn-ITAL Association ,and inacost-sharing
contract inthe field ofnuclear medicine .MEDIVINE
δ
k
5

-7Χ1Ι/368/73-Ε
Nomodification oftheauthorized staff isproposed .
This proposal ,oflimited scope ,isintended tosetupthe first
ties between thelaboratories oftheSix (orsome ofthem )and those
ofthenewMember States ,and onthestrength ofpromising results fee
farobtained ,todevelop certain aspects ofthepreviously adapted
programme .

XII/3S8/73-E
PROPOSAL FOR ACOUNCIL DECISION AMENDING THE DECISION OF
IkMAY 1973 ADOPTING AFIVE-YEAR RESEARCH AND TRAINING
PROGRAMME OFTHE EUROPEAN ATOMIC ENSRC-Y COMMUNITY INTHE
FIELD OFBIOLOGY AND HEALTH FEYSICS
THECOUNCIL OFTHEEUROPEAN COMMUNITIES ,•
Having regard totheTreaty establi^ing theEuropean Atomic Energy
Community ,and inparticular Article 7thereof ;
Having regard totheCommission 'sproposal concerning aprogramme about
which theScientific an3Technical Committee hasbeen consulted ; ?
Whereas theprogramme which isthesubject ofthe Council .Decision of
^May1973 'adopting afive-year research andtraining programme of
theEuropean Atomic Energy Community in .the field ofbiology a.c.dhealth
physics should headjusted on ttheoccasion ofthe?accession ofnew
Member States tothe .Community ;
Whereas theproposal from theCommission constitutes anadequate adjustment
which would strengthen theexisting Cemmunity 'spotential inthe field of
research onnuclear methods and techniques applicable tomedical and
agricultural research ,and whereas theexploitation ofthis potential
presents great interest inthis area ;
Whereas this proposal ensures thecontinuity vfcich isnecessary forbio­
logical research ;
Whereas itisinthecommon interest toamend thedecision of1^May 1973
with aview toensuring theactive participation ofthelaboratories ©f
theenlarged Community countries inresearch work ;
HAS DECIDED ASFOLLOWS :
10JN°L153,9June 1973 ,p.15.

ΧΙΤ/368/73-Ξ
Article 1
Theprogramme setout intheAnnex tothedecision of i-iay1973 shall x
bereplaced bytheprogramme annexed tothis decision .
Article 2
Article 2ofthedecision of1*fMay 1973 shall bereplaced bythe
following :
"Theupper limit forexpenditure commitments and forstaff necessary
fortheimplementation ofthis programme shall be18886million units
ofaccount and 97community servants inthe case oftheRadiation
protection sector and 7^10million units ofaccount and 10Community
servants inthe case oftheApplications sector ,theunit ofaccount
being defined intheFinancial Regulation concerning theestablishment
andimplementation ofthebudget oftheEuropean Communities and the
responsibility oftheauthorizing andaccounting officers ".
\

XII/3S3/73-E
ANNEX
"BIOLOGY AND HEALTH PHYSICS "
PROGRAMME
"Eadii.tion protecticn "sector
Anamount of18886million units ofaccount shall beallocated tothis
objective and theupper limit forstaff shall befixed at97servants .
Theaim ofthework istoacquire and promote thescientific and technical
knowledge necessary fordetermining permissible levels ofradiation inman
andcontamination oftheenvironment ,forkeeping such knowledge uptodate
and forimproving thepractical organization ofradiation protection bythe
Menber States .
This aim also includes studies ofradioactive contaminants ,with parti­
cular reference tothepath which they follow inmanand theenvironment ,
ontheeffects ofradiation onliving matter and ondosimetric methods
andinstruments . , •
This research will becarried outmainly under contracts ofassociation
orsimilar contracts andpartly bythe "Biology "Group setupatthe
Ispra Establishment .
"Application "sector
(a)Definition ofprogramme
Anamount of7410 million units ofaccount shall feeallocated tothe
objective ,and theupper limit forstaff shall be10Community servants
Theaim ofthework consists inthedevelopment ofnuclear techniques
and theapplication ofnuclear methods with aview totheir use in
agricultural andmedical research .
The work will becarried out under contracts ofassociation orsimilar
contracts .

XII/368/73-E
ANNSX 1
"SUPPLEMENTARYPPOGBA^ME "APPLICATIONS "
Outline of"Applications ofnucleartechniques to.agricultural research "
1.Gr*)UT)ofcontracts inthefieldofradioentomoloa-y
1.1.Johannes Gufcenberg University »Mainz,Gernany
Institute ofGenetics (Prof.H.Laven)
development ofgeneticsystems (semi-sterility chromosome
translocations )forcontrolofinsectpests.-
, .»
1.2.EavarianInstitute forSoils,.PlantsandPlantPathology Munich
(Dr.A.Haisch)
Control ofthecherryfruitflywiththeuseofdsmlrant lethal
factors
-Laboratory andmassbreeding ofthecherryfruitfly
-Ecologyandsterilisation ofthecherryfruitfly.
1.3*Institute forPlantpathological research (IPO),Wageningen
TheNetherlands (Dr.J.Ticheler )• '
Controloftheonionfly;fcythesterile-male technique and
thetranslocation technique (including fieldtests)
1.4.ComJ.ta.toNazionale' perl'EnergiaNucleare ,Reme,Italy
Laboratory forAgricultural Applications (Prof.A.Eosszini)
ricj.og5.cal controleftheMediterranean -fruitfly"bythe
sterile-male technique andfield-scale experiment onProcida
island,
1>5*University ofPaidova,Italy .
Institute forAgricultural Entomology (Pref.S.Zangheri )
Methodsandtechniques formassbreeding oftheoliveflyand
ofotherinsectpests(bacterial symbiosis ,nutritive media,
population dynamics).

i-2– XII/368/73-E
Annex 1
N»B .(a)Prof. R.Cavalloro (Biology Group oftheCommission atlepra )
I
works xnclose cooperation with theUniversity ofPadova on
some particular radiobiological problems oftheolive flyand
theMediterranean fruit fly .- .
(b)Theradioentqmology projects ofITAL (seeunder point £)
areincluded inthesame programme astheabove group of
contracts .
•Gro^p ofcontracts inthe field ofplant ,cell culture
2.1 aUniversity ofPisa *Italy
Institute ofGenetics (Prof. F.S'Amato )
Cytology and genetics of pj.p-.nt tissue an'!cells grown invltrw
(differentiation anddeaif ferentiation ,production ofprotoplasts
and ofhaploids ).
2.2 ,Gomitata Nazionale per l'Energìa Nucleare .Rome .Italy
Latoratery foragricultural applications (Prof. A.Bozzini )
Applications ofinvitre cultures ofhigher plant cells to
radiobiolog icalandmutagenetic research (self-incompatibility
problems ,modifications atthemacromolecular level ,genetic
studies onisogenic lines oftobacco ).
2*3 «Gesellschaft fürStrahlen-und Umweltforschung ,Munich ,Germany
Department ofPlant Genetics (Prof .H.Gaul )
Production and utilizatian ofhaploids ,inbarley ,maize and potato
2.4 .Free University ofBerlin ,"'Germany
Institute ofPlant Physislogy (Prof. J.Reinert )
Growth anj differentiation of "giant cells and tissue grown invitro
(production ofhaplcid ,protoplasts andfusion ).

-3- XII/368/73-E
Annex 1
Association EUR/.TOM^-ITAL ,Wag"ninflen .theNetherlands (Dr. D-q".-seuw)
-Movnant ofselectedsv.bstancos andele:~*?nts Insoils
(ioniccomposition .ofthesoilsolution,;transport ofsoilmoisture ,
behaviour ofphc3x>hate andmercury,denitrification andN-fixation ).
-Uptake,transport andaccumulation .ofelements .ic.'plants•rAiwufcuq –fcn–wn– iwwmw*– immiam■■■■––ann'nurtB iinumtsa»
(ionuptakebyrootsfromdilutedsolutions ,kinetics ofionuptake,
uptakeandreleaseofionsbysubcellular structures ,localisation
inplanttissue).
-Mutaiion breeding ,incompatibility 3,rautagenssis
(nutation breeding bytheadventitious budtechnique ,biochemical
andgeneticaspectsofself«=inco;npat±b:i.lity ).
-F~odirradiation
(radioresist&nce ofmicroorganisms andstores,metabolism ofbacteria ,
1
radiation.-and heateffects onenzymes).
"Genetic control ofinsectpasta
(Adoxophyes orana,Tetranychus urticad -&ndHylemiagntiqua).
Instrument research aridmethodology development isalsocarriedout
atIT'AL(semi-conductor assemblies ,microautoradiography methods,
instrumentation forionuptakekinetics study).
SuhcorstrexctnoftheSURATOM-I'rAI .:Association
4.1«Gesellschaft fürStrahlen-und Umweltforschung ,Munich,Germ&ny
Department ofPlantGenetics (Prof.H.Gaul) *
Production andselection ofbarley-withavertical andhorizontal
resistance against mildew,
-withanincreased protein
quantity andimproved proteinquality.

XII/363/73-E
Anixzs .1
H>2„University 01Bonn ,Germany
Institute ofGenetics (Prof. W.Gottscbalk )
Physiological and biochemical genetics ofPisura sativum
(particularly seed pretein andbalance control mechanisms ).
*K3»Catholic University ofNijmegen ,theNetherlands
Faculty ofMathematics andSciences ,Laboratory ofBotany
(Prcf .H.F. Linskens )
Biochenlatry ofstyles and pollen ofincompatible plants .
*f»^.Agricultural University ,Wageningen ;theNetherlands
Institute ofPlant breeding (Prof. J.Sneep )
Sporophytic incompatibility ,radiation induction ofdisease resistance
inwheat andbarley .
Comitato T'azionale per l'Energia Nucleare ,Rome ,Italy
Laboratory ofAgricultural Applications (Eof .A.Bozzini )
Genetic andbiochemical studies ofself-incoapatibili f-.y,(work performed
partly atI'TAL andpartly atLaC&saccia ),study ofinduced and
spontaneous mutations fordisease resistance intomato andhard wheat .
4-»6 .University ofBari ,Italy . .., •
Institute ofPlant Breeding (Pr^f .G.T. Scarascia-Mugnoaza )
Induction ofmutations fordiseaca resistance incommercial varieties
ofPisum sativum .
^«7.Istituto Sperimentalo perlaCerealicoltura ,Rome ,Italy (Prof .A.
Bianchi )
Qualitative improvement ofplant prot-oin with special reference to
opaque maize .
M-oO .University ofModena ,Italy
Institute ofGeneral Pathology (Prof. E.Carafoli )
Uptake nndrelease ofjensbymitochondria *

1-5- Xï1/368/73-E
Annex 1
^«9-Gesellschaft fürSirahlen-und Umweltforschung »Mu.nich,Germäny
Institute ofRadiation Bot&ny.Hannover (Dr.W.Kühn)
Measurement ofplant-nassinfieldconditions ,detoction oftree
diseases insitu,moisture measureme nt'inthinsoillayers.
^•10.University ofStrasbourg ,France .
Laboratory ofRadiation Eiophysics andMethodology (Dr.S»Hechenmann )
Development ofhigh-efficiency andhigh-resolution ionopraphic
methods,andtiieirapplication toautoradiographic prebl-sms *'

"•6- XII/368/73-E
Ann*?r 1
Out .l3 .aeof"Applications ofnuclear techniques tomedical research "
1.University o£Ulm .Germany
Nuclear Medicine Section (Prof *W.E. Adam )
Development ofanon-traumatizing technique fortheanalysis of
cardiac kinotics * -
2»University ofPisa ,School ofNuclear Medicine (Prof. L.Donato )
-Development ofradio^lnuiunological me-chod sofmeasuripg the
proteic andnon-proteic constituents ofplasma .
-Development anduse ofshort~liv?d isotopes for thequantitative
analysis ofthelung andkidney functions ,and ofcoronary circulations ,
5.Gesellschaft fürStrahlen-und Umweltforschung ,Munich ,Germany
Institute ofHematology ,andDepartment ofImmunology (Dr.S.Thierfelde *
1M-Control with "I-TgG ofthepersistance oftolerance toantilymphocytfe
globulin (ALG )inpatients treated with ALG .
-Kinetics find clinical effects ofblood cellsisolated byNCI-IBM cell
gecaratcr andstored bycryobiological methods .
-Regulation anddisturbances ofhemoglobin metabolism inman .
Grcup-ontract on tvDosimetry-Neutron ther&py "with following establish­
ments :
-Gesellschaft fv.r Str.ahlen-und Umwelt forschung ,Munich
Institut fürStrahlens chuts (Dr.Bürger ).
-Gesellschaft fürStrahlen-und Umwelt forschung ,Munich
Abteilung f'drBiophysikalische Strahlenforschung (Prüf .Pohlit )
»M.R»C .Hammersmith Hospital .London (Dr*Bewley ).
-T.N.O. RadiobiologicsJ .Institute »Rijswijk (Dr.Broerse )»

-7 XTI/368/73-E
Anne *1
-International Comsissica fcr Radiation Units and Measurements
(I.C.B.U. ).
Inrelation with fast neutron therapy :
-preparation andimplementation ofdose comparisons ,and development
ofmethods formeasurement ofabsorbed doses andneutron spectra®

X.II/368/73-E
ANNEX 2 ■
'PLANT CFJX CULTURE AND USE "■ ';
Invitro plant cell culture ,particularly ofhaploid cells ,opons a
range ofnewprospects fortheinvestigation offundamental genetic
mechanisms and forthebreeding ofimproved varieties .Butthepossi­
bilities offered bythis technique cannot beexploited without knowledge
ofaseries ofparaiseiers which determine thedevelopment oftheceli-
ticcue-plant sequence ,affect theploidy level andinfluence its stabili
ana induce differentiation end dedif forentiation .
Theprogramme currently under way inGerman and Italian laboratories
includes thefollowing research subjects J
~haploid production from anthers and from haploid calluses ,
«biocheaical andmoleculaz *aspects ofcell differentiation invitro ,
-study ,onamacromolecular level ,ofchanges induced intissues of
various plants byinvitro culture ,
-genetic study ofisogenic lines andapplication ofmutagenic treatment
tomicrospores ofdiploid isogenic lines ,
»use ofcell culture inthestudy ofself-incompatibility ,
-production ofprotoplasts and their somatic fusion .
Byrxplolflr .,~the potential oflaboratory * inth?countries not vet
parr i.cipati-jg inthis proprramae itwould bepossible tostudy mora
thoroughly some cfthesubjects already being tackled ,and tocomplete
theprogramme with original lines ofapproach *Asmain example a*
-detailed study ofthephysiological ar.dgenetic conditions necessary
fortriggering androgenesis and thedevelopment ofertsbryoids ,
-production ofhap.loids through interspecific crosses ,
-production anduse ofisogenic lines ,

•2~ XII/368/73-E
ANNEX 2
-production ofprotoplasts andsomatic hybridization from somatic
ceils and pollen tetrads .
Bycoordinating theresearch done intheEuropean laboratories which are
working onthese subjects itvould bepossible toform anoriginal group ,
large enough totackle theproblems and prospects ofplant cell culture
inadecisive manner .

ΧΙΙ/363/73--Έ
ANIIEX3
INCOMPATIBILITY IN'HIGHER PLANTSX
^-–lnw fWM–»«P– –– »•
Incompatibility Can-1compatibility )inaplantlisreflected ini£a
incapacity (orcapacity )forself-fertilization *■■»Cont£ol .of'this \
feature and.ofthenschanismg involvedisveryimportant bothfor
understanding thegeneticstructure ofthespeciesand-for."exploiting
itinpractice forbreeding purposes .Experimental ind'Uflitxon ;ofself-
compatibility isalsoneededinmanyallogamous species ,forhonozygotic
selection ofinteresting combinations ofdominant andrecessive charac­
teristics ..Furthermore ,the"c'ontrcf^syid ^ifappropriate ,theintroduction
ofeslf-incompatibility incert'afitycrqps,isessential "for"largescale
production ofhybridplantswith.a-Liglidegreeofheterosis .
~\'0" '.•'•
t «
*» '
Λ .»· #«1 ·1'/«Ι'... ''■«Pese&rch csurrentdLy underwayintheCommunity pt"okra.fflme concerns both
>iiwhii ■>wil»«iiii–h miIfann–i ■ illnia■■■■■–i»wiim mhiih iiBiwJt i <-na»
thefundamental andthepractical aspectsofincompatibility *
Different approaches arebeingusedforanalysing themechanism of
incompatibility :
••/ % •;V . . . '! .'é
'if.-. 1
''.a^^ -
•biochemical :studyofinyitrosynthesis ofS-proteins ,roleof
sugarsinthespecifity of<S-glycoproteins .analysis ofradiation-
inducedinhibition ofself^incompatibility and .ofthecompetitive-'
effectindiploidordigenicpollen,elaboration ofabiochemical ,"
mo£elofself-in'com*patibility ..•,-
■···.· ' ;.·'··"''"*0-cytologxcal :identification ofthechromosome bearingtheincompa­
tibility Slocv.3andstudyoftheroleofthenucleolar organized in
theexpression ofself-incompatibility .
/
-physical :electron microscopy studyoftheultrastructure ofthe
incompatibility reaction .
Workontheexploitation ofincompatibility inbreeding andmutagenesis
covers ?
-induction ,analysis andexploitation ofself-compatibility in.
allogamous lines,

-2- XI1/368/73-E
ANNEX 3
-development ,analysis andexploitation ofinterspecific hybrids
between self-»coaipatible lines andwild self-incompatible related lines ,
-early detection ,forbreeding purposes ,ofdifferent S-genotypes ,
-analysis ,formutation breeding purposes ,ofthemutation spectra at
theS-locus and ,where appropriate ,identification oftherare mutagenic
circumstances leading totrue intragenic constructive mutations
(generation ofnewS-apecificities ).
Research ,inthenewMember State s,follows certain lines which provide
agood complement tothose described above .Among them :
-biochemical and physiological malysis ofself-incorapatibility in
sporophytic systems ,and electron microscopy studies ,
-production ofself-incompatible strains innormally self-compatible
cereal grains ,
-research onthegeneration ofnewalleles attheS-locus ofself -
incompatible strains submitted toobligate inbreeding ,
-use ofthe S-systera forproducing hybrid seed ,interspecific hybri­
dization ,andselection forself-compatibility .
Itshould beemphasized that a.great deal ofthis work iscarried outon
sporophytic systems oronmulti-loci mechanisms ,while thepresent
Community programme deals mainly with monofactorial gametophytic species .
Complementarity isthus quite obvious .

XII/368/73-E
ANNEX k -
OPTIMI ZAT'T ONOFCULTIVATION TECHNIQUES
Thedevelopment ofagriculture and cultivation practices incur countries
will beincreasingly influenced byeconomic planning atCommunity level
which tries toextrapolate into the future theaspirations and trends of
to-day 'ssociety .Among these ,thereduction ofagricultural manpower ,
better preservation ofnatural resources and rationalization oftheir
useareliable toinfluence future cultivation techniques directly .But
these cannot berationalized andadapted tofuture trends without a
through knowledge oftheinteractions between the components ofthe
artificial ecosystems ofmodern agriculture .
Without denying theneed todiversify cultivation techniques according to
regional tecological ,economic orsociological conditions ,itseems that
considerable iftteres thas been aroused bythe results obtained sofarbya
series ofprogressive techniques andmethods known as''reduced tillage "*
Experiments already carried out inCommunity countries and elsewhere have
demonstrated that in'certain conditions yields obtained byreduced tillage
arecomparable tothose obtained bytraditional methods ?andthat ,inthe
long run ,animprovement of "soil structure and better utilisation ofits
water andnutrient content canbeexpected .Nevertheless ,itiscertain
that further research isneeded todetermine more precisely thfoptimum
conditions of ,reduced tillage ."
The renin problems tobestudied invarious soil typos and various
Climates are : \
-influence ofreduced tillage on'themain physical' andchemical soil '
parameters ;,structure and evolution ofcompactness ,"water behaviour ,
aeration ,fertilizer -mainly Nitrogen -behaviour andutilization ,;..
organic matter ;
-root growth under reduced tillage ,roct distribution inprofiles ,r
physiological activity ,root -shoot -yield relations ,reactions to ;j
various stresses . r- <

~c"" XXI/3?8/73-E
ANHFX 4
-microflora activity andearthworm population patterns ,
-adaptation ofherbicide use toconditions prevailing undor reduced
cultivation .
-dxsposal ofcrop residue ,
-crop rotations ,
»development ofbetter adapted machinery and techniques .
Research along these lines requires amultidiscipl5..nary approach involving
soil physicists ,agricultural chemists ,physiologists ,plant pathologists „
geneticists andagricultural engineers .
Byitsnature ,this research will have tobecarried out ondifferent leve
butalldirected toward thesame general target .Thebuilding ofmodels ,
tocomplete existing ones ,must beaimed atmastering thecomplex interact
between climate andsoil conditions ,andplant growth .Experiments in
controlled and serai-artificial conditions must quantify theparameters whi
determine climate -soil -plant relations }long-term field experiments in
varied andrepresentative soils must compare evolution ofsoils ,plant
populations and yields intraditional andreduced cultivation conditions .
Institutes already participating intheCommun ityprogramme have avaried
range ofscientific skills and technical facilities which could beused ia
aresearch effort such asthat described above .
Incooperation with "other Institutes ,theCommission proposes toestablish ,
acoherent research programme :
-todetermine theconditions ofapplication ofreduced tillage technique -
tomeet the .requirements oftomorrow 'sagricultural economy ,
-touseadvanced methods and techniques inamultidisciplinary approach ,
-toconcentrate ontheplanning andimplementation ofexperiments in
controlled conditions andinthefield ,inabroad range ofsoils and
climates ,end tostudy theeffects ofreduced cultivation onthechemici ;
andphysical conditions ofthesoil ,onroot development ,and onyields o

ZIl/^6d/75-E
ANNEX 5
FAST NEUTRO *!T'ERIPY
Agrowing number ofradiotherapy units are interested inthefundamental
and practical aspects cfthe use offast neutrons inclinical radiotherapy .
First results provide strong evidence oftheusefulness ofthis treatment .
Butrapid progress intheevaluation ofits possible advantages ishampered
bythelimited number ofcases available inthebeginning which are
suitable forneutron treatment .Close cpoperation between the different
centres using this therapy istherefore very desirable .The Cowrciseion
proposes therefore toextend itsaction onneutron dosimetry and inter -
comparison towards theevaluation ofbasic radiobiological and clinical
aspects offast neutron therapy .
Inorder toenable theevaluation ofpossible advantages within a
reasonable period oftine and toprevent waste oftime and money in
all countries involved ,aEuropean cooperation onfast neutron radio­
therapy has been established under thesponsorship ofthe European
Organization forResearch onTreatment ofCancer (EORTC ).Ten centres
participate inthis project ontheclinical application offast neutrons .
TheCommission proposes tosupport thefollowing aspectss
a)Asasupport forthebasic aspects oftheclinical programme ,acute
and late effects offast neutron treatments will beinvestigated'
with various biological systems .
b)Exchange ofvarious types ofbiological systems ,including tumors ,
which can beemployed asbiological dosimeters toevaluate the
qualitative and quantitative differences ofthe various neutron
beams tobeemployed .
c)Classification ofthetypes ofhuman tumors which areexpected to
besuitable forneutron radiotherapy .

r2- XII/7'G3/75-E
ANNÏÏX 5
'd)Evaluation ofthetreatment schedules tobeapplied .
e)Quantitative assessment ofresponses ofnormal tissues andtumors
inpatients .
f)Close cooperation onwell-defined clinical trials offast neutron
radiotherapy . •'
g)Inanadditional project anintercomparison study will becon­
tinued onthe fast nfeutron dosimetry atthevarious institutes
(seeAnnex 6).

XII/3Ô8/73-E
ANNEX 6
NETJTKON DOSIMETRY AND INTERCOMPARISON
Oneimportant prerequisite forcomparisons ofclinical results of
fast neutron radiotherapy ismaximum knowledge about thephysical
conditions ofirradiation .Therefore theCommission supports since
197?aprogramme onneutron dosimetry andintercomparison .
Four institutes (including aninstitute intheUK)have been collabo­
rating under agroup contract oncertain research projects and are
preparing anintercomparison intheCommunity ,based onexperience
gained inanICSU intercomparison .
The Commission proposes :
toincrease thenumber ofinstitutes participating intheresearch
.projects , '
tocontinue supporting thepresent ICRU intercomparison and the
participation ofalimited number ofEuropean institutes ,init,
tocontinue preparation oftheEuropean intercomparison which is
open toallinterested institutes intheCommunity .
Theresearch projects areconcerned with measurement ofthebiologically
effective neutron dose .They include :
theinvestigation ofdosimetric anil radiobiological problems ,
calculation oftheabsorbed dose andneutron energy spectra
inside biological matter ,
theapplication ofmicrodosimetric data andresults forevaluation
and elaboration ofneutron irradiation conditions ,
theuse ofvarious experimental techniques andneutron detectors ,
including different types ofbiological dosimeters forneutron
measurement ,
theinvestigation ofthe relative biological effectiveness of
neutron irradiation ,

-2- XII/368/7VE
ANNEXb
theexamination ofthepossibilities ofstandardization of
irradiation andmeasuring "techniques ,
\
theexamination ofthepossibleroleofheavychargedparticles
intherapy.
TheICKtTIntercomparison istakingplaceonaworldscale,withthe
participation ofalimited numberofEuropeaninstitutes working on
neutronradiobiology andneutrontherapy.Theprojectisbeing
carried ou.tattheBrookhaven National Laboratory .
TheCommunity-wide intercomparison offastneutrondosimetry willbe
carriedoutlateronalargerscale.Allinterested institutions will
beinvitedtoparticipate .TheRadiobiological Institute TNOandthe
Institut fiirStrahlenschutz GSFareprepared tooffertheirneutron
radiation facilities (monoenergetic neutrons withenergies between
100keVand15MeV)forthisEuropean Intercomparison Projectwhose
successdepends ontheexperience gainedinthefirstICRUinter-
comparison .
Theaimoftheprojectistocomparetheresultsofvariouslabora­
toriesandresearch groupsperforming fastneutrondosimetry and
usingsimilarordifferent techniques insituations approximating
thosegenerally encountered inradiotherapy andradiobiology .
Dosimetry ofycontamination willalsobecarriedout.