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COMMISSION REGULATION ( EEC ) No 3480/90

of 30 November 1990

amending Regulation ( EEC ) No 1725/79 on the rules for granting laid to skimmed milk processes into compound feedingstuffs and skimmed-milk powder intended in particular for feed for calves

THE COMMISSION OF THE EUROPEAN COMMUNITIES,

Having regard to the Treaty establishing the European Economic Community,

Having regard to Council Regulation ( EEC ) No 804/68 of 27 June 1968 on the common organization of the market in milk and milk products ( 1 ), as last amended by Regulation ( EEC ) No 3117/90 ( 2 ), and in particular Article 10 ( 3 ) thereof,

Whereas Article 10 ( 3 ) of Commission Regulation ( EEC ) No 1725/79 ( 3 ), as last amended by Regulation ( EEC ) No 2871/90 ( 4 ), states that the skimmed-milk powder has to be determined by testing each sample at least in duplicate in accordance with the method of analysis specified in Annex III; whereas it is desirable to extend the field of application to compound feedingstuffs even containing less than 50 % skimmed-milk powder;

Whereas the analytical procedure has to be modified in order to eliminate possible sources of error;

Whereas a correction of the analytical data by a factor obtained from regression analysis leads to more accurate results;

Whereas the repeatability and reproducibility of the new procedure have to be determined;

Whereas a tolerance limit should be given in order to facilitate the interpretation of analytical results;

Whereas the measures provided for in this Regulation are in accordance with the opinion of the Management Committee for Milk and Milk Products,

HAS ADOPTED THIS REGULATION :

Article 1

Regulation ( EEC ) No 1725/79 is hereby amended as follows :

1 . in Article 10 ( 3 ), the first subparagraph is replaced by the following :

"The results of the inspections referred to in Article 2 ( 2 ) and in paragraph 2 ( a ), ( b ) and ( c ) of this Article shall be recorded by the agency responsible for inspection in the analysis report and inspection report, specimens of which appear in Annexes I and II . The quantity of skimmed-milk powder shall be determined by testing each sample at least in duplicate in accordance with the method of analysis specified in Annex III . Where such inspections relate to skimmed-milk powder to be used, whether as such or in the form of a mixture, the absence of rennet whey powder is proven by the procedure outlined in Annex IV .';

2 . Annex III is amended as set out in the Annex hereto .

Article 2

This Regulation shall enter into force on the day of its publication in the Official Journal of the European Communities .

It shall apply from 1 January 1991 .

This Regulation shall be binding in its entirety and directly applicable in all Member States .

Done at Brussels, 30 November 1990 .

For the Commission

Ray MAC SHARRY

Member of the Commission

( 1 ) OJ No L 148, 28 . 6 . 1968, p . 13 .

( 2 ) OJ No L 303, 31 . 10 . 1990, p . 5 .

( 3 ) OJ No L 199, 7 . 8 . 1979, p . 1 .

( 4 ) OJ No L 275, 5 . 10 . 1990, p . 20 .

ANNEX

amending Annex III to Regulation ( EEC ) No 1725/79

The following points are hereby amended as set out below :

1.22 .

Replace "50 %' by "10 %'.

3.3 .

Replace "Commisison Directive 72/199/EEC of 27 April 1972' by "IDF 20A 1986 '. //

Insert "( see 9.1 )' after "27,5 %'.

4.2 .

Replace by : "Calcium chloride ( 2M solution ). Weigh 20,018 g of CaCO3 ( analytical grade ) in a porcelain capsule of suitable size ( 150 to 200 ml ) or in a beaker . Cover with distilled water and transfer onto a boiling water bath . Add slowly 50 to 60 ml of HCI solution ( conc . HCI : water = 1 : 1 ) to solubilize the carbonate completely . Keep on the boiling water bath until the CaCI2 is dried, to eliminate the HCI which has not reacted . Transfer with distilled water to a 100 ml measuring flask and dilute to the mark . Control the pH value, which must be not lower than 4,0 . Store the solution in a refrigerator .'

4.6 .

Replace "Commission Directive 72/199/EEC of 27 April 1972' by "IDF 20A 1986 '.

6.3.1 .

Replace "1 ml' by "3,4 ml '.

6.3.2 . to 6.4.1 .: Replace by the following text :

1.2.3"6.3.2 .

Transfer the liquid into one ( or two ) certifuge tubes and centrifuge at 2 000 g for 10 minutes in order to remove the precipitated material . Transfer the supernatant, without washing the sediment, into one ( or two ) centrifuge tubes . //

6.3.3 .

Bring the temperature of the supernatant back to 37 *C . While stirring the extract, add, dropwise, 0,5 ml of the liquid rennet ( 4.5 ). Coagulation appears in one or two minutes . //

6.3.4 .

Return the sample to the water bath and leave at a temperature of 37 *C for 15 minutes . Remove the sample from the bath and break the coagulum by stirring . Centrifuge at 2 000 g for 10 minutes . Filter the supernatant through a suitable filter paper*, Whatman No 541 or equivalent, retain the filter paper . Wash the precipitate in the centrifuge tube with 50 ml of water at approximately 35 *C by stirring the precipitate . // //

Centrifuge again at 2 000 g for 10 minutes . Filter the supernatant through the filter paper retained previously . // // // // //

(*) A fast filtering ashless paper should be used . //

6.4 .

Determination of casein nitrogen . //

6.4.1 .

After washing, transfer quantitatively the precipitate to the filter paper retained from 6.3.4 using distilled water . Transfer the filter paper to the Kjeldahl flask . Determine the nitrogen by the Kjeldahl method as described by IDF 20A 1986 .'

1.27.1 .

Replace "Commission Directive 72/199/EEC of 27 April 1972' by "IDF 20A 1986 '.

9.1 .

Replace by : "The percentage of skimmed-milk powder in the compound feedingstuff is calculated by the following formula :

1.2.3.4.5.6.7 // //

(

N x 6,38 27,5

x 100

)

_ 2,81

1.2.3,7 //

% SMP = // // //

0,908

1.2Where N is the percentage of para-casein nitrogen; 27,5 is the factor for converting determined casein into the percentage of skimmed-milk powder; 2,81 and 0,908 are correction factors obtained from regression analysis .

10.1 .

Repeatability : //

"90 %' is replaced by "95 %'; //

"2 g' is replaced by "2,3 g '.

10.2 .

Reproducibility : //

"90 %' is replaced by "95 %'; //

"5 g' is replaced by "6,5 g '.

The following is inserted under point 11 :

"11 . Tolerance limit :

The CrD95-value ( critical difference; 95 % confidence limit ) is calculated using the formula ( ISO 5725 ):

1.2.3.4.5.6.7CrD95 =

1 § 2

§ R2 _ r2

(

n_1 n

)

( R : reproducibility; r : repeatability )

Double determination : CrD95 = 4,5 g'

Where the result of the chemical analysis differs from the declared content of skimmed-milk powder by not more than 4,5 g ( double determination ) the consignment of compound feedingstuff is deemed to comply with this provision of the Regulation .'

Existing point 11 becomes point 12 .