Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation

mg active substance/kg of complete feed with a moisture content of 12 %

Category: Sensory additives. Functional group: Flavouring compounds

2b139-eo - Expressed lemon essential oil Additive composition Expressed lemon essential oil from fruit peel of Citrus limon (L.) Osbeck Liquid form Chickens for fattening - - 35 1. The additive shall be incorporated into the feed in the form of a premixture. 2. In the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment shall be indicated. 3. Mixture of expressed lemon essential oil with other botanical additives is permitted provided that the amounts of perillaldehyde, furocoumarins, and methoxycoumarins in feed materials and compound feed are lower than the one resulting from the use of a single additive at the maximum or recommended level for the species or animal category. 4. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks by inhalation, dermal contact or eyes contact. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eye and breathing protection. 29 September 2032

Turkeys for fattening Salmonids 40

Characterisation of the active substance Expressed lemon essential oil obtained by cold expression from fruit peel of Citrus limon (L.) Osbeck as defined by the Council of Europe (1) d-Limonene: 60–73 % β-Pinene (pin-2(10)-ene): 9–18 % γ-Terpinene: 6–12 % α-Pinene (pin-2(3)-ene): 1,3–3,0 % Sabinene (4(10)-thujene): 0,3–3,0 % Geranial: 0,1–2,0 % Neral: 0,1–1,8 % Perillaldehyde: ≤ 0,023 % Furocoumarins: ≤ 0,3 % Methoxycoumarins: ≤ 0,06 % CAS number 84929-31-7 FEMA number 2625 CoE number: 139 Laying hens 52

Pigs for fattening 74

Piglets 62

Sows 92

Calves (milk replacers) Cattle for fattening Dairy cows 90

Analytical method (2) For the quantification of phytochemical marker d-limonene in the feed additive or in mixture of flavouring compounds: — Gas chromatography coupled with flame ionisation detection (GC-FID) (based on ISO 855) Horses 137

Sheep/Goats Rabbits 30

2b139- rf - Residual fraction of expressed lemon oil distilled Additive composition Residual fraction of expressed lemon oil distilled from fruit peel of Citrus limon (L.) Osbeck Liquid form Chickens for fattening - - 11 1. The additive shall be incorporated into the feed in the form of a premixture. 2. In the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment shall be indicated. 3. Mixture of residual fraction of expressed lemon oil distilled with other botanical additives is permitted provided that the amounts of perillaldehyde, furocoumarins, and methoxycoumarins in feed materials and compound feed are lower than the one resulting from the use of a single additive at the maximum or recommended level for the species or animal category. 4. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks by inhalation, dermal contact or eyes contact. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eye and breathing protection 29 September 2032

Laying hens Turkeys for fatening Rabbits Salmonids 12

Characterisation of the active substance Residual fraction of expressed lemon oil distilled obtained by the distillation of lemon essential oil expressed from fruit peel of Citrus limon (L.) Osbeck The specifications of the active substance are: d-Limonene 5989-27-5 01.045 51–63 53.3 49.3–56.8 c-Terpinene 99-85-4 01.020 8–17 16.9 12.8–23.3 Geranial 141-27-5 05.188 6–12 10.4 9.5–11.2 Neral 106-26-3 0.8724 5–9 7.8 6.2–8.9 b-Pinene (pin-2(10)-ene) 127-91-3 01.003 0.3–5.5 1.24 0.32–3.38 b-Bisabolene 495-61-4 01.028 0.3–4 d-Limonene: 51–63 % γ-Terpinene: 8–17 % Geranial: 6–12 % Neral:5–9 % β-Pinene (pin-2(10)-ene): 0,3–5,5 % β-Bisabolene: 0,3–4 % Perillaldehyde: ≤ 0,092 % Furocoumarins: ≤ 0,8 % Methoxycoumarins: ≤ 0,22 % CoE number: 139

Ruminants 20

Piglets 20

Pigs for fattening 24

Sows 30

Horses 35

Analytical method (2) For the quantification of phytochemical marker d-limonene in the feed additive or in mixture of flavouring compounds: — Gas chromatography coupled with flame ionisation detection (GC-FID) (based on ISO 855)

2b139-di - Distilled lemon essential oil (volatile fraction) Additive composition Distilled lemon essential oil (volatile fraction) obtained from lemon essential oil expressed, from fruit peel of Citrus limon (L.) Osbeck Liquid form Chickens for fattening - - 36 1. The additive shall be incorporated into the feed in the form of a premixture. 2. In the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment shall be indicated. 3. Mixture of distilled lemon essential oil (volatile fraction) with other botanical additives is permitted provided that the amounts of perillaldehyde, furocoumarins, and methoxycoumarins in feed materials and compound feed are lower than the one resulting from the use of a single additive at the maximum or recommended level for the species or animal category. 4. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks by inhalation, dermal contact or eyes contact. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eye and breathing protection. 29 September 2032

Laying hens 53

Rabbits 56

Turkeys for fattening 48

Characterisation of the active substance Distilled lemon essential oil (volatile fraction) obtained by lemon essential oil expressed, from fruit peel of Citrus limon (L.) Osbeck as defined by the Council of Europe (1) The specifications of the active substance are: d-Limonene: 66–78 % β-Pinene (pin-2(10)-ene): 5–20 % γ-Terpinene: 1,5–9,5 % α-Pinene (pin-2(3)-ene): 0,5–3,0 % Sabinene: 0,3–3,0 % Furocoumarins: ≤ 0,1 mg/kg Methoxycoumarins: ≤ 0,1 mg/kg CoE number: 139 Piglets 64

Pigs for fattening 76

Sows 94

Calves (milk replacers) Cattle for fattening Sheep/Goats 95

Horses 141

Analytical method (2) For the quantification of phytochemical marker d-limonene in the feed additive or in mixture of flavouring compounds: — Gas chromatography coupled with flame ionisation detection (GC-FID) (based on ISO 855) Dairy cows 91

Salmonids Ornamental fish Dogs 60

Cats 30

2b141-eo - Distilled lime essential oil Additive composition Distilled lime essential oil obtained by unpeeled fruits of the plant species Citrus aurantiifolia (Christm.) Swingle Liquid form Chickens for fattening - - 8,5 1. The additive shall be incorporated into the feed in the form of a premixture. 2. In the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment shall be indicated. 3. Mixture of distilled lime essential oil with other botanical additives is permitted provided that the amounts of perillaldehyde, furocoumarins, and methoxycoumarins in feed materials and compound feed are lower than the one resulting from the use of a single additive at the maximum or recommended level for the species or animal category. 4. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks by inhalation, dermal contact or eyes contact. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eye and breathing protection. 29 September 2032

Laying hens 12,5

Turkeys for fatening 11

Piglets 15

Characterisation of the active substance Distilled lime essential oil obtained by steam distillation from unpeeled fruits of the plant species Citrus aurantiifolia (Christm.) Swingle as defined by the Council of Europe (1) The specifications of the active substance are: d-Limonene: 45–52 % γ-Terpinene: 10–14 % Terpinolene: 5,5–10,5 % αΤerpineol: 6–8 % β-Caryophyllene: 0,2–0,8 % Furocoumarins: ≤ 0,0083 % Methoxycoumarins: ≤ 0,03 % CoE number:141 Pigs for fattening 18

Lactating sows 22

Cattle for fattening 33,5

Calves (milk replacers) 35,5

Dairy cows 21,5

Sheep/Goats Horses 33,5

Rabbits 13,5

Salmonids Ornamental fish 30