Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation

Units of activity of additive/kg of fresh material

Technological additives: silage additives

1k101 Alpha-amylase (EC 3.2.1.1) Additive composition Preparation of alpha-amylase produced by: Bacillus amyloliquefaciens DSM 9553, having a minimum activity of 129 800 DNS (1)/g additive Solid form Characterisation of the active substance Alpha-amylase produced by Bacillus amyloliquefaciens DSM 9553 Analytical method (2) For the determination of alpha-amylase in the feed additive: colorimetric (DNS) method based on the enzymatic hydrolysis of the starch at pH 4,5 and 37 °C All animal species — — — 1. In the directions for use of the additive and premixture, the storage conditions and stability to heat treatment shall be indicated. 2. Minimum dose of alpha-amylase when used without combination with other enzymes or microorganisms as silage additives 40 DNS/kg of fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing protection. 11 April 2029

1k102 Alpha-amylase (EC 3.2.1.1) Additive composition Preparation of alpha-amylase produced by Bacillus amyloliquefaciens NCIMB 30251, having a minimum activity of 101 050 DNS/g additive Solid form Characterisation of the active substance Alpha-amylase produced by Bacillus amyloliquefaciens NCIMB 30251 Analytical method (2) For the determination of alpha-amylase in the feed additive: colorimetric (DNS) method based on the enzymatic hydrolysis of the starch at pH 4,5 and 37 °C All animal species — — — 1. In the directions for use of the additive and premixture, the storage conditions and stability to heat treatment shall be indicated. 2. Minimum dose of alpha-amylase when used without combination with other enzymes or microorganisms as silage additives 10 DNS/kg of fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing protection. 11 April 2029

1k103 Alpha-amylase (EC 3.2.1.1) Additive composition Preparation of alpha-amylase produced by Aspergillus oryzae ATCC SD-5374 having a minimum activity of 235 850 DNS/g additive Solid forms Characterisation of the active substance Alpha-amylase produced by Aspergillus oryzae ATTC SD-5374 Analytical method (2) For the determination of alpha-amylase in the feed additive: colorimetric (DNS) method based on the enzymatic hydrolysis of the starch at pH 4,5 and 37 °C All animal species — — — 1. In the directions for use of the additive and premixture, the storage conditions and stability to heat treatment shall be indicated. 2. Minimum dose of alpha-amylase when used without combination with other enzymes or microorganisms as silage additives 23 DNS/kg of fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing protection. 11 April 2029