552 OfficialjournaloftheEuropeanCommunities
21.7.71 OfficialJournaloftheEuropeanCommunities NoL163/41
REGULATION (EEC)No1539/71OFTHECOMMISSION
of19July1971
determiningCommunitymethodsfortheanalysisofwines
THECOMMISSION OFTHEEUROPEAN COM­
MUNITIES ,procedures;whereas,however,inviewofcommercial
requirementsandlimitations,arestrictednumberof
simplifiedproceduresshouldbepermittedenabling
therequisitefactorstobe.determinedrapidlyand
withreasonableaccuracy;the HavingregardtotheTreatyestablishing
EuropeanEconomicCommunity ;
Whereasgenerallyrecognisedmethodsshouldbe
used;whereas,therefore,maximumuseshouldbe
madeofthemethodsformulatedunderthe1954
International Convention fortheUnification of
MethodsfortheAnalysisandAppraisalofWines;
WhereasthemeasuresprovidedforinthisRegulation
areinaccordancewiththeOpinionofthe
ManagementCommitteeforWine;
HASADOPTEDTHISREGULATION :
Article1HavingregardtoCouncilRegulation (EEC)No
816/701of28April1970layingdownadditional
provisionsforthecommonorganisationofthemarket
inwine,aslastamendedbyRegulation (EEC)No
2612/702andinparticularArticle20(4)andArticle
39thereof;
HavingregardtoCouncilRegulation (EEC)No
817/703of28April1970layingdownspecial
provisionsforqualitywinesproducedinspecified
regions,andinparticularArticle11(4)thereof;
WhereasArticle39ofRegulation (EEC)No816/70
providesfortheadoptionofdetailedrulesnecessary
forimplementingtheprovisionsofAnnexesIandII
andinparticulartheadoptionof'methodsof
analysis;
WhereasArticle11(1)ofRegulation (EEC)No
817/70providesforananalyticaltest,includingan
assessmentofthe.characteristics ,aslistedinthe
AnnextothatRegulation,ofthequalitywinep.s.r.in
question;whereasparagraph3ofthatsameArticle
envisagestheadoptionofCommunitymethods;
Whereastheprovisionsrelatingto>oenological
processesandconditionsforreleasetothemarket
callforaneffectivecontroloftheproductsin
question;whereastheuniformapplicationofsuch
controlandtheverificationofdocumentary
particularsofproductscallfortheintroductionof
uniformmethodsofanalysistoensurethataccurate
andcomparableinformationisobtained;whereas,
consequently ,thesemethodsshouldbecompulsory
forallcommercialtransactionsandallverification1.Themethodsofanalysisfortheapplicationof
Regulations (EEC)Nos816/70and817/70shallbe
thosesetoutintheAnnextothisRegulation.
ExceptwhereexpresslystatedinthatAnnex,such
methodsshallbeappliedasdirectedintheAnnexto
theCompendium ofInternationalMethodsforthe
AnalysisofWines,preparedandsupplementedunder
the1954International ConventionfortheUnification
ofMethodsfortheAnalysisandAppraisalofWines.
2.Fortheitemsforwhichbothstandardmethods
andsimplifiedmethodsarelaid/down,results
obtainedfromthestandardmethodsshallprevail.
Article2
1OJNoL99,5.5.1970,p.1.
2OJNoL281,27.12.1970,p.6.
3OJNoL99,5.5.1970,p.20.ThisRegulationshallenterintoforceon1September
1971.

OfficialJournaloftheEuropeanCommunities 553
ThisRegulationshallbebindinginitsentiretyanddirectlyapplicableinallMember
States.
DoneatBrussels,19July1971.
FortheCommission
ThePresident
FrancoM.MALFATTI
ANNEX
1.Fordeterminingspecificgravityanddensity:
(a)thestandardmethodshallbemeasurement byapycnometer
(b)thesimplifiedmethodshallbemeasurement byahydrometer .
ThesemethodsaredescribedunderA1(c)intheAnnextotheCompendium referredtoin
Article1.
2.Fordeterminingtheactualalcoholicstrength:
(a)ofwines:
(aa)thestandardmethodshallbedoubledistillationandmeasurement ofthespecific
gravityofthedistillatebyapycnometer ;
(bb)thesimplifiedmethodshallbesimpledistillationoftheneutralisedliquidand
measurement ofthealcoholicstrengthbyahydrometer ;.
(b)ofmustandmustinfermentationwithanactualalcoholicstrengthnotexceeding6°:
(aa)thestandardmethodshallbethemeasurement ofalcoholbychemicalmeans,
(bb)thesimplifiedmethodshallbethatgivenunder(a)(bb).
ThesemethodsaredescribedunderA2(c)intheAnnextotheCompendiumreferredtoin
Article1.
3.Thetotaldryextractshallbemeasuredbyadensimeterandcalculatedindirectlyfromthe
specificgravityoftheresiduewithoutalcohol.
ThismethodisdecribedunderA3(c)intheAnnextotheCompendiumreferredtoinArticle1.
4.Thereducingsugarcontentshallbedeterminedasfollows:
(a)byclarification :
—ofredwines:withleadsubacetate,
—ofotherproducts:withzincferrocyanide ;
(b)byallowingtheclarifiedwinetoreactwithafixedquantityofanalkalinecoppersalt
solution,andmeasuringtheexcessofcopperionsbyiodometry.
ThesemethodsaredescribedunderA4(d)intheAnnextotheCompendiumreferredtoin
Article1.
5.Fordeterminingthesucrosecontent:
(a)thestandardmethodshallbe:
(aa)forqualitativetesting:thinlayerchromatography andpaperchromatography ,
(bb)formeasurementafterinversion;methodoutlinedunder4;

554 OfficialJournaloftheEuropeanCommunities
(b)thesimplifiedmethodshallbe:
(aa)forqualitativetesting:colorimetry .
(bb)formeasurementafterinversion:methodoutlinedunder4.
ThesemethodsaredescribedunderA5(f)intheAnnextotheCompendiumreferredtoin.
Article1. ^ -
Wheretheresultobtainedfromthemeasurement ofthesucrosecontentislessthan1g/1for
drywinesor2g/1forsweetwines,aqualitativetestshallbecarriedout.Ifthisgivesanegative
result,itisnotattributabletothesucrose.
6."Ashcontentshallbedeterminedbyincineratingthedryextractatbetween500°Cand550°C
untilcompletecombustionofthecarbonhastakenplace.
ThismethodisdescribedunderA6(a)intheAnnextotheCompendiumreferredtoin
Article1.
7.Themethodsofdeterminingthealkalinityoftheashshallbe:
(a)inrespectoftotalalkalinity:backtitrationwithsulphuricacidafterheatingusingmethyl
orangeasanindicatortowhich,ifnecessary,adropofmethylenebluemaybeadded;
(b)inrespectofpartialalkalinity:backtitrationwithsulphuricacidinthepresenceofasmall
excessofcerousionsusingacolourindicatorchangingatpH4-5.
ThosemethodsaredescribedunderA7(a)intheAnnextotheCompendium referredtoin
Article1.
8.Fordeterminingthetotalacidity:
Afterfirstremovingthecarbonicacidandsulphurdioxide:
(a)thestandardmethodshallbe.potentiometrictitration,
(b)thesimplifiedmethodshallbetitrationwithbromothymol bluetoindicatetheendofthe
reaction.
Thetotalacidityshallbeexpressedinmilli-equivalents perlitre.
ThesemethodsaredescribedunderA10(a)intheAnnextotheCompendiumreferredtoin
Article1.
9.Thevolatileacidityshallbedeterminedbyseparationofthevolatileacidsbysteamdistillation.
Thecolourindicatorusedshallbephenolphthalein .
Thevolatileacidityshallbeexpressedinmilli-equivalents perlitre.
ThismethodisdescribedunderA11(a)intheAnnextotheCompendium referredtoin
Article1.
10.Thenon-volatileacidityshallbedeterminedbycalculatingthedifferencebetweenthetotal
acidityandthevolatileacidity.
11.Fordeterminingtartaricacid:
(a)thestandardmethodshallbecolorimetricmeasurementofthetartaricacid—isolatedby
meansofananionexchangecolumn—bymeansoftheredcolorationwhichitgiveswith
vanadicacid;
(b)thesimplifiedmethodshallbeacidimetricmeasurementofthetartaricacidprecipitatedin
theformofpotassiumacidtartrateunderconditionswhichreducesolubilitytoaminimum.
ThesemethodsaredescribedunderA12(g)intheAnnextotheCompendiumreferredtoin
Article1.
12.Citricacidshallbedeterminedwiththeotheracidsbyananionexchangeresin.Thecitramalic
acidisseparatedbyfractionalelution.Thecitricacidisconvertedbyoxidizationintoacetone
whichisseparatedbydistillation.Theacetoneismeasuredbyiodometry.
ThismethodisdescribedunderA29(e).intheAnnextotheCompendium referredtoin
Article1,

OfficialJournaloftheEuropeanCommunities 555
13.Fordeterminingsulphurdioxide:
(a)thestandardmethodshallbemeasurementofthetotalsulphurdioxide,carriedoverbya
currentofairornitrogenintoastandardsolutionofsodiumhydroxide;
(b)thesimplifiedmethodshallbe:
(aa)inthecaseoffreesulphurdioxide,directiodometrictitrationwithablanktitrationof
thesamewineinwhichthefreesulphurdioxidehasbeencombinedbyanexcessof
ethanalorpropanal;
.(bb)inthecaseofcombinedsulphurdioxide,iodometrictitrationafterdoublealkaline
hydrolysisofthewinewherethefreesulphurdioxidehasbeenoxidizedinthecourse
oftheiodometrictitration;
(cc)inthecaseofthetotalsulphurdioxide,byaddingtheresultsobtainedinthetwo
analysesreferredtoin(aa)and(bb)
ThesemethodsaredescribedunderA17(c)intheAnnextotheCompendiumreferredtoin
Article1.
14.ThepHvalueofthewineandthatofthemustshallbedeterminedbymeasuringthedifference
inpotentialbetweentwoelectrodesdippedintheliquidunderexamination .Oneofthese
electrodeshasapotentialwhichisacertainfunctionofthepHoftheliquid,theotherhasa
fixedandknownpotentialandconstitutesthereferenceelectrode.
ThismethodisdescribedunderA31(f)intheAnnextotheCompendium referredtoin
Article1.