Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method. Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation

mg/kg of complete feed with a moisture content of 12 %

Category of nutritional additives. Functional group: amino acids, their salts and analogues

3c364 - L-arginine Additive composition: Powder with a minimum content of L-arginine of 98 % (on a dry matter basis) and a maximum content of 15 % water All animal species - - - 1. L-arginine may be placed on the market and used as an additive consisting of a preparation. 2. In the directions for use of the additive and premixture, the storage conditions and the stability to heat treatment shall be indicated. 3. The moisture content shall be indicated on the label of the additive. 4. For users of the additive and premixture, feed business operators shall establish operational procedures and organisational measures to address potential risks for the eyes and skin and by inhalation. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixture shall be used with personal protective equipment. 5 August 2030

Characterisation of the active substance: L-arginine ((S)-2-amino-5-guanidinopentanoic acid) produced by fermentation with Corynebacterium glutamicum NITE SD 00285. Chemical formula: C6H14N4O2 CAS number: 74-79-3

Analytical method (1): For the identification of L-arginine in the feed additive: — Food Chemical Codex ‘L-arginine monograph’ For the quantification of arginine in the feed additive: — ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-VIS) For the quantification of arginine in premixtures, compound feed and feed materials: — ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-VIS) – Commission Regulation (EC) No 152/2009 (Annex III, F)

3c362 - L-arginine Additive composition: Powder with a minimum content of L-arginine of 98 % (on a dry matter basis) and a maximum content of 0,5 % water All animal species - - - 1. L-arginine may be placed on the market and used as an additive consisting of a preparation. 2. The additive may also be used via water for drinking. 3. In the directions for use of the additive and premixture, the storage conditions, the stability to heat treatment and the stability in water for drinking shall be indicated. 4. Declaration to be made on the label of the additive and premixture: ‘The supplementation with L-arginine, in particular via water for drinking, should take into account all essential and conditionally essential amino acids in order to avoid imbalances.’ 5. For users of the additive and premixture, feed business operators shall establish operational procedures and organisational measures to address potential risks for skin and eyes. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixture shall be used with personal protective equipment. 5 August 2030

Characterisation of the active substance: L-arginine ((S)-2-amino-5-guanidinopentanoic acid) produced by fermentation with Corynebacterium glutamicum KCCM 80182 Chemical formula: C6H14N4O2 CAS number: 74-79-3

Analytical method (2): For the identification of L-arginine in the feed additive: — Food Chemical Codex ‘L-arginine monograph’ For the quantification of arginine in the feed additive and water: — ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-VIS) For the quantification of arginine in premixtures, compound feed and feed materials: — ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-VIS) – Commission Regulation (EC) No 152/2009 (Annex III, F)

Category: Sensory additives. Functional group: Flavouring compounds

3c362 - L-arginine Additive composition: Powder with a minimum content of L-arginine of 98 % (on a dry matter basis) and a maximum content of 0,5 % water All animal species - - 1. L-arginine may be placed on the market and used as an additive consisting of a preparation. 2. The additive shall be incorporated into the feed in the form of a premixture. 3. In the directions for use of the additive and premixture, the storage conditions and the stability to heat treatment shall be indicated. 4. On the label of the additive the following shall be indicated: ‘Recommended maximum content of the active substance of complete feedingstuff with a moisture content of 12 %: 25 mg/kg.’ 5. The functional group, the identification number, the name and the added amount of the active substance shall be indicated on the label of the premixtures, if the following content of the active substance in complete feedingstuff with a moisture content of 12 % is exceeded: 25 mg/kg. 6. For users of the additive and premixture, feed business operators shall establish operational procedures and organisational measures to address potential risks for skin and eyes. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixture shall be used with personal protective equipment. 5 August 2030

Characterisation of the active substance: L-arginine ((S)-2-amino-5-guanidinopentanoic acid) produced by fermentation with Corynebacterium glutamicum KCCM 80182 Chemical formula: C6H14N4O2 CAS number: 74-79-3 Flavis 17.003