1977D0795 — EN — 05.06.2003 — 002.001 — 1
This document is meant purely as a documentation tool and the institutions do not assume any liability for its contents
►B COUNCILDECISION
of12December 1977
establishingacommon procedure for theexchange ofinformationonthe qualityofsurface fresh
waterintheCommunity
(77/795/EEC)
(OJ L 334, 24.12.1977, p. 29)
Amended by:
Official Journal
No page date
►M1Council Decision of 19 October 1981 (81/856/EEC) L 319 17 7.11.1981
►M2CommissionDecisionof 24 July 1984 (84/422/EEC) L 237 15 5.9.1984
►M3Council Decision of 24 November 1986 (86/574/EEC) L 335 44 28.11.1986
►M4CommissionDecisionof 14 December 1989 (90/2/EEC) L 1 20 4.1.1990
►M5Council Regulation (EC) No 807/2003 of 14 April 2003 L 122 36 16.5.2003
Amended by:
►A1Act of Accessionof Greece L 291 17 19.11.1979
►A2Act of Accessionof Spainan d Portugal L 302 23 15.11.1985
►A3Act of Accession of Austria, Sweden and Finland C241 21 29.8.1994
(adapted by Council Decision 95/1/EC, Euratom, ECSC) L 1 1 1.1.1995
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COUNCILDECISION
of12December 1977
establishing a common procedure for the exchange of information
onthequalityofsurfacefreshwaterintheCommunity
(77/795/EEC)
THE COUNCIL OF THE EUROPEAN COMMUNITIES,
Having regard to the Treaty establishing the European Economic
Community, and in particular Article 235 thereof,
Having regard to the proposal from the Commission,Having regard to the opinion of the European Parliament (
1),
Having regard to the opinion of the Economic and Social
Committee (2),
Whereas the 1973 (3) and 1977 (4) programmes of actionof the
European Communities on the environment provide for the introductionof a procedure for the exchange of information between the pollutionsurveillance and monitoring networks;
Whereas such a procedure is necessary to determine the pollution
levels of the rivers in the Community and consequently to lay down
guidelines for the control of pollution and nuisances, which is one ofthe Community's objectives in respect of the improvement of livingconditions and the harmonious development of economic activitiesthroughout the Community; whereas no provision is made in the Treatyfor the specific powers required for this purpose;
Whereas such an exchange of information on pollution levels is one of
the means of monitoring the long-term trends and the improvements
resulting from the application of current national and Community rules;
Whereas the exchange of information provided for in this Decision
should allow for as significant a comparison as possible of the resultsobtained in the sampling and measuring stations;
Whereas the exchange of information provided for in this Decision
would lay the foundations for a system for monitoring surface fresh-water pollution at Community level and could constitute a componentof the global environmental monitoring system provided for in theUnited Nations environment programme;
Whereas to attainthese objectives the Member States must forward to
the Commissiondata relatin g to certainparameters for surface fresh
water; whereas the Commissionwill draw up a con solidated reportwhich it will transmit to the Member States;
Whereas the list of stations in Annex I may, with advantage, be modi-
fied by the Commission at the request of the Member State concerned,provided that certaincriteria are fulfilled;
Whereas technical progress requires that the technical specifications
laid down in Annex II to this Decision should be adapted promptly;whereas, to facilitate the implementation of the measures required for
this purpose, provisionmust be made for a procedure establishin g close
cooperationbetweenthe Member States an d the Commissionwithinthe Committee for the adaptationof this Decisionto techn ical progress,1977D0795 — EN — 05.06.2003 — 002.001 — 2
(1) OJ No C 178, 2. 8. 1976, p. 48.
(2) OJ No C 285, 2. 12. 1976, p. 10.
(3) OJ No C 112, 20. 12. 1973, p. 3.
(4) OJ No C 139, 13. 6. 1977, p. 3.
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HAS ADOPTED THIS DECISION:
Article 1
A common procedure for the exchange of information on the quality of
surface fresh water inthe Commun ity is hereby established.
Article 2
1. For the purposes of this Decision sampling or measuring stations'
means the stations listed in Annex I.
2. The information concerning the parameters listed in the first
column of Annex II covered by the exchange of information shall be:
(a) the results of the measurements carried out by the sampling or
measuring stations;
(b) a description of the sampling, sample preservation and measuring
methods used and the frequency of sampling.
Article 3
1. Each Member State shall designate a central agency and inform
the Commissionthereof within15 days of the n otificationof this Deci-
sion.
2. The information referred to in Article 2 (2) shall be forwarded to
the Commission through the central agency in each Member State.
3. The data referred to inArticle 2 (2) (a) shall be presen ted
according to the modes of expression and with the significant figures
set out in the second and third columns of Annex II.
The descriptions of the methods referred to in Article 2 (2) (b) may be
omitted if the methods are the same as those used inprevious years,
provided always that an explicit reference to any such omission is
made;
4. The information, covering a calendar year, shall be forwarded to
the Commissionbefore 1 October of the followin g year.
5. The Commission shall forward annually to the Member States
which so request the information received under paragraph 2. Every
three years, and for the first time in 1987, the Commission shall drawup a draft consolidated report based on the information referred to inArticle 2 (2). The part of this report concerning the informationsupplied by a Member State shall be sent to the central agency of thatState for verification. Any comments on the draft shall be included in
the report. The report shall include indications of the trends noted in
water quality since implementation of this Decision and as much inter-pretative comment as possible, taking into account the objectives of theDecision.
The Commission shall publish the final version of its report and send
copies to the Member States.
6. The Commissionshall assess the effectiven ess of the procedure
for the exchange of information and shall submit proposals, whereappropriate, to the Council aimed at improving the procedure and, ifnecessary, harmonizing the methods of measurement, taking intoaccount the provisions of Article 4.
Article 4
1. Each Member State shall organize such intercalibration at
national level amongst laboratories taking part in the collection andthe analysis of data as may be necessary to ensure comparability ofreference methods of measurement with those used in the laboratoriesof the Member States.1977D0795 — EN — 05.06.2003 — 002.001 — 3
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2. The Commission shall, if necessary, organize a comparative
evaluation of nationally applied methods of measurement. This evalua-tionshall be the subject of a report to be tran smitted to Member States.
3. Onthe basis of the report referred to inparagraph 2, the Commis-
sion shall, if necessary, make proposals to the Council for theintercalibration of nationally applied methods of measurement withthe reference methods of measurement listed in Annex III.
Article 4a
1. In order to implement the common procedure for the exchange of
information, the Member States shall lay down a frequency forsampling and analysis, normally monthly.
2. If a Member State has established that the water quality does not
display any significant variation in terms of the value of one or more
parameters and if there is no risk of a deterioration of the water quality,the frequency of sampling and measurement of that parameter or thoseparameters may be reduced. Such a reduction of the frequency shall notinvolve any risk to human beings and the environment.
Modifications to the frequency shall be explicitly mentioned in the
information forwarded to the Commission under Article 3 (2).
3. The reference methods of measurement for the parameters in
question are set out in Annex III. Laboratories which use othermethods of measurement shall satisfy themselves that the results
obtained are comparable.
4. The containers used for samples, the agents or methods used to
preserve part of a sample for the analysis of one or more parameters,the conveyance and storage of samples and the preparation of samplesfor analysis must not be such as to bring about any significant change
inthe results of the an alysis.
5. Sampling shall take place in the same places and the sampling
procedures shall be the same oneach occasion .
Article 5
1. The list in Annex I may be amended by the Commission on a
request from the Member State concerned.
2. The Commission shall make such amendments when it is satisfied
that the following requirements are met:
— that the list of sampling or measuring stations for each Member
State is sufficiently representative for the purposes of this Decision,
— that the stations are at points which are representative of water
conditions in the area around and not directly and immediatelyinfluenced by a source of pollution,
— that they are capable of measuring at regular intervals the para-
meters in Annex II,
— that they are as a general rule not more than 100 kilometres apart
on main rivers, not including tributaries,
— that they are upstream of any confluences and not on tidal stretches
of water.
3. The Commission shall inform the Council of any amendments
which it has accepted.
4. The Commission shall submit for decision by the Council any
requests for amendments which it has been unable to accept.
Article 6
Amendments necessary to adapt to technical progress the list of para-
meters and the modes of expression and significant figures in respectthereof set out in Annex II, as well as the reference methods ofmeasurement, the parameters and the modes of expression set out in1977D0795 — EN — 05.06.2003 — 002.001 — 4
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Annex III, shall be adopted in accordance with the procedure laid down
in Article 8, provided that any additions to the list involve only para-meters covered by Community rules concerned with the aquatic
environment and for which data are available in all sampling and
measuring stations of the Member States. Any changes in the modesof expression and significant figures must not involve changes to themethods of measurement used by the Member States in the variousstations in Annex I.
Article 7
1. A Committee for the adaptationof this Decisionto techn ical
progress (hereinafter referred to as the ‘the Committee’) is hereby set
up, consisting of representatives of the Member States with a represen-tative of the Commissionas Chairman .
Article 8
1. The Commissionshall be assisted by the committee for the adap-
tationof this Decisionto techn ical progress.
2. Where reference is made to this Article, Articles 5 and 7 of Deci-
sion1999/468/EC (1) shall apply.
The period laid downinArticle 5(6) of Decision1999/468/EC shall be
set at three months.
3. The committee shall adopt its rules procedure.
Article 9
This Decisionis addressed to the Member States.1977D0795 — EN — 05.06.2003 — 002.001 — 5
(1) OJ L 184, 17.7.1999, p. 23.▼B
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ANNEX I
LIST OF SAMPLING OR MEASURING STATIONS INVOLVEDIN THE EXCHANGE OF
INFORMATION
FEDERAL REPUBLIC OF GERMANY
Sampling or measuring stations List of rivers
Maxau 362·3 km downstream of the Rhine bridge at Constance Rhine
Mainz 498·5 km downstream of the Rhine bridge at Constance Rhine
Koblenz/Brau-
bach590·3 km downstream of the Rhine bridge at Constance Rhine
Palzem 230·3 km upstream of the confluence with the Rhine Moselle
Koblenz/
Moselle2 km upstream of the confluence with the Rhine Moselle
Kleve/Bimmen 864·95 km downstream of the Rhine bridge at Constance at the
point where the river leaves the Federal Republic of GermanyRhine
Duisburg 1,0 km upstream of the confluence with the Rhine Ruhr
Herbrüm 284·9 km from the source (212·04 Canal km) Ems
Hemeln 11 km downstream of the confluence of the Werra and the
FuldaWeser
Intschede 329·7 km downstream of the confluence of the Werra and the
FuldaWeser
Geesthacht 113 km downstream of the entry of the river into the Federal
Republic of Germany (584·5 Elbe km)Elbe
Jochenstein 2 203 km upstream of the mouth of the Danube Danube
BELGIUM
Sampling or measuring stations List of rivers
Warneton downstream of the confluence with the Deule Lys
Leers-Nord at the point where the river enters Belgium Espierre
Doel at the point where the river leaves Belgium ScheldtBléharies at the point where the river enters Belgium Scheldt
Erquelinnes at the point where the river enters Belgium Sambre
Heer-Agimont at the point where the river enters Belgium Meuse
Lanaye-
Ternaaienat the point where the river leaves Belgium Meuse
Martelange at the point where the river leaves Belgium Sure
Zelzate at the point where the river leaves Belgium Ghent-
Terneuzen
Canal
DENMARK
Sampling or measuring stations List of rivers
Nåby StationNo 57.12 Suså
Nr Broby StationNo 45.01 Oden se Å
Tvilum bro StationNo 21.01 Guden å
Ahlergård StationNo 25.05 SkjernÅ1977D0795 — EN — 05.06.2003 — 002.001 — 6
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GREECE
Sampling or measuring station List of rivers
Drama Papades Bridge (01) (30 km from the point where the river
enters Greece)Nestos
Serres Sidirokastro Bridge (02) (18 km from the point where the river
enters Greece)Strymon
Thessaloniki Kilometre post 61 · 500 on the Thessaloniki-Idomeni railway
line (03) (10 km from the point where the river enters Greece)Axios
Kozani Moni Ilarion (04) upstream of the Polyphyte reservoir (150 km
upstream of the mouth)Aliakmon
Larissa Larissa municipality water-tower (05) (50 km upstream of the
mouth)Pinios
Agrinion Kastraki (06) downstream of the reservoir (50 km upstream of
the mouth)Akheloos
FRANCE
Sampling or measuring stations List of rivers
Méry upstream of the confluence with the Aube (St 6 000) Seine
Montereau upstream of the confluence with the Yonne (St 14 000) Seine
Ponthierry Bridge RD 50 (St 48 000) SeineParis Tolbiac Bridge (St 81 000) Seine
St Rambert Andrézieux Bridge downstream of St Rambert (St 9 000) Loire
Brienon downstream of the Pouilly-sous-Charlieu Bridge RN 487 R.G.
(St 15 000)Loire
Orléans upstream of Orléans (St 50 500) Loire
La Possonière SNCF bridge downstream of La Possonière (St 134 500) Loire
Toulouse downstream of Toulouse (St 161 000) Garonne
Lamagistère downstream of the confluence with the Aveyron (St 117 000) Garonne
Couthures near Couthures, downstream of the confluence with the Avance
(St 81 000)Garonne
Auxonne France Bridge (St 11 000) SaôneLyon upstream of the confluence with the Rhône at the Mazaryk
Bridge (St 59 500)Rhône
Pont de
Pougnyat the point where the Rhône enters France (St 65 700) Rhône
Lyon upstream of the confluence with the Saône at the Poincaré
Bridge (St 93 000)Rhône
St Vallier upstream of the confluence with the Isère (St 104 000) Rhône
IRELAND
Sampling or measuring stations List of rivers
Slane Bridge approximately 12 km downstream of Navan (N 96 74) Boyne
CorofinBridge approximately 19 km down stream of Tuam (M 42 43) Clare
Graiguenama-
nagh Bridgeapproximately 29 km downstream of Muine Bheag (Bagenals-
town) (S 71 44)Barrow
Killavullen
Bridgeapproximately 13 km downstream of Mallow (W 65 99) Blackwater
(Munster)1977D0795 — EN — 05.06.2003 — 002.001 — 7
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ITALY
Sampling or measuring stations List of rivers
Bronzolo-
Ponte Vadena300 km upstream of the mouth Adige
Trento 253 km upstream of the mouth Adige
Badia Polesine 66 km upstream of the mouth Adige
Moncalieri 594 km upstream of the mouth PoCremona 281 km upstream of the mouth Po
Boretto 216 km upstream of the mouth Po
Borgo Forte 184 km upstream of the mouth Po
Pontelagoscuro 91 km upstream of the mouth Po
Ponte degli
Alberi38 km upstream of the mouth Metauro
BuonRiposo 186 km upstream of the mouth Arn o
Nave di
Rosano120 km upstream of the mouth Arno
Capraia 70 km upstream of the mouth ArnoCalcinaia 38 km upstream of the mouth ArnoPonte Felcino 300 km upstream of the mouth Tiber
Ponte Nuovo 273 km upstream of the mouth Tiber
Ponte Ripetta 43 km upstream of the mouth Tiber
GRAND DUCHY OF LUXEMBOURG
Sampling or measuring stations List of rivers
Wasserbillig upstream of the confluence with the Moselle Sûre
NETHERLANDS
Sampling or measuring stations List of rivers
Lobith at the point where the river enters the Netherlands Upper Rhine
Kampen 123 km downstream of the point where the Rhine enters the
NetherlandsIjssel
Vuren 88 km downstream of the point where the Rhine enters the
NetherlandsUpper
Merwede
Hagestein 82 km downstream of the point where the Rhine enters the
NetherlandsLek
OM 42 Puttershoek, 120 km downstream of the point where the Rhine
enters the NetherlandsOude Maas
NM 34 near the island of Brienenoord, 134 km downstream of the point
where the Rhine enters the NetherlandsNieuwe Maas
Eijsden 4·5 km downstream of the point where the Meuse enters the
NetherlandsMeuse1977D0795 — EN — 05.06.2003 — 002.001 — 8
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Sampling or measuring stations List of rivers
Lith 201 km downstream of the point where the Meuse enters the
NetherlandsMeuse
Keizersveer 248 km downstream of the point where the Meuse enters the
NetherlandsBergse Maas
H 9 Haringvlietbrug (Haringvliet Bridge) Haringvliet
H 12 near the Haringvlietdam Haringvliet
IJ 12 Ketelbrug, 145 km downstream of the point where the Rhine
enters the NetherlandsKetelmeer
IJ 23 centre of Lake Ijssel Lake Ijssel
UNITED KINGDOM
Sampling or measuring stations List of rivers
Chollerford 6 km upstream of the confluence (NY 919 706) North Tyne
Warden Bridge 800 m upstream of the confluence (NY 910 660) South Tyne
Wylam Bridge immediately upstream of the tidal limit (NZ 119 645) Tyne
Derwenthaugh 1·3 km upstream of the tidal limit (NZ 187 607) Derwent
Whitford
Bridge3 km upstream of the tidal limit (SY 262 953) Axe
Tregony
Gauging
Station6 km upstream of the tidal limit (SW 921 445) Fal
Devoran
Bridgeimmediately upstream of the tidal limit (SW 791 394) Carnon
Denny Bridge 0,5 km upstream of the tidal limit (SD 504 647) Lune
St Michael's
Weirimmediately upstream of the tidal limit (SD 462 411) Wyre
Samlesbury 1·5 km upstream of the tidal limit (SD 589 304) Ribble
Teddington
Weir(TQ 171 714) Thames
Chetwynd (SK 187 138) Tame
Nottingham (SK 581 383) Trent
Yoxall (SK 131 177) Trent
Fochabers 4 km upstream of the tidal limit (NJ 341 596) Spey
Craigiehall 3 km upstream of the tidal limit (NT 165 752) Almond
Renton Foot-
bridgeimmediately upstream of the tidal limit (NS 389 783) Leven
SPAIN
Sampling or measuring stations List of rivers
SanEstebande
Gormazstationno02.07 Douro
Villamarciel stationno02.54 Douro
Puente Pino station no02.53 Douro
Trillo stationno03.93 Tagus
Aranjuez station no03.11 Tagus
Talavera de la
Reinastationno03.15 Tagus1977D0795 — EN — 05.06.2003 — 002.001 — 9
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Sampling or measuring stations List of rivers
Alcántara station no03.19 Tagus
Balbuena station no04.08 Guadiana
Badajoz stationno04.18 Guadiana
Menjibar station no05.04 Guadalquivir
Peñaflor stationno05.06 Guadalquivir
Sevilla stationno05.74 Guadalquivir
Miranda de
Ebrostationno09.01 Ebro
Zaragoza stationno09.11 Ebro
Tortosa stationno09.27 Ebro
PORTUGAL
Sampling or measuring stations List of rivers
Lanhelas Station no01.1 Minho
Messegães Stationno01.4 Minho
Barragem de
CrestumaStationNo 09.2 Douro
Pinhão Station No 09.6 Douro
S. João de
LoureStationno12.2 Vouga
Penacova Station no16.4 Mondego
Santarém Station no30.3 Tagus
Perais Stationno30.10 Tagus
Castelo de
BodeStationno30.20.2 Zêzere
Mértola Stationno54.3 Guadiana'
Sada Ajuda Stationno57.7 Guadiana'
AUSTRIA
Sampling or measuring stations List of rivers
Jochenstein 2 203,8 km upstream of the mouth Danube
Abwinden-
Asten2 119,9 km upstream of the mouth Danube
Wolfsthal 1 873,5 km upstream of the mouth Danube
Lavamünd 2,1 km upstream the point where the Drau leaves Austria Drau
Kufstein/Erl 204,03 km upstream of the confluence with the Danube Inn
Oberndorf 47,2 km upstream the confluence with the Inn Salzach
Bad Radkers-
burg101,4 km upstream the confluence with the Drau Mur
FINLAND
Sampling or measuring stations List of rivers
Kalkkisten-
koskiStation No 4800, lake outlet of lake Päijänne Kymi1977D0795 — EN — 05.06.2003 — 002.001 — 10
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Sampling or measuring stations List of rivers
Pori-Tampere
BridgeStationNo 8820, 7,5 km upstream of Pori Kokemäen joki
Mansikkakoski Station No 2800, lake outlet of lake Saimaa Vuoksi
Raasakka
Bridge8,0 km upstream of Ii Ii
Merikoski
BridgeStationNo 13000, Oulu City Oulujoki
Isohaara
BridgeStationNo 14000, Kemi City Kemijoki
Kukkolankoski Station No 14310, 13 km upstream of Tornio Torniojoki
Virtaniemi Station No 14400, lake outlet of Lake Inari Paatsjoki
SWEDEN
Sampling or measuring stations List of rivers
Luleå StationNo 009 Lule älv
Stornorrfors Station No 028 Ume älvBergeforsenStation No 040 In dalsälven
Älvkarleby StationNo 053 Dalälven
Stockholm StationNo 061 Norrström
Norrköping Station No 067 Motala ström
Mörrum StationNo 086 Mörrumsån
Helsingborg Station No 094 Råån
Laholm StationNo 098 Lagan
AleyckanStation No 108 Göta älv1977D0795 — EN — 05.06.2003 — 002.001 — 11
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ANNEX II
PARAMETERSINRESPECTOFWHICHINFORMATIONISTOBEEXCHANGED
(Modesofexpressionandsignificantfiguresfortheparametric data)
ParameterMode of expres-
sionSignificant figures
Before the decimal
commaAfter the decimal
comma
physicalRate of flow(1) (at the
time of sampling)m3/sec xxxx xx
Temperature ºC xx x
pH pH xx x
Conductivity at 20 ºC µS cm−1(<100) xx
(≥100) xxx
chemicalChlorides Cl mg/l(<100) xx
(≥100) xxx
Nitrates NO3mg/l xxx xx
Ammonia NH4mg/l xxx xx
Dissolved oxygenO2mg/l xx x
BOD5O2mg/l xxx x
COD O2mg/l xxx x
Total phosphorus P mg/l xx xxSurfactants reacting to
methylene blueSodium lauryl
sulphate eq.
mg/lxx xx
Total cadmium Cd mg/l x xxxx
Mercury Hg mg/l x xxxx
microbiologicalFaecal coliforms / 100 ml xxxxxx
Total coliforms (
2) / 100 ml xxxxxx
Faecal streptococci (2) / 100 ml xxxxxx
Salmonella (2)/ 1 l x
biological biological quality (2)(3)
(1) The date of sampling must be given.
(2) The data relating to this parameter shall be exchanged when it is measured.
(3) The frequency of sampling of this parameter and the mode of expression of results shall be decided on by
Member States.1977D0795 — EN — 05.06.2003 — 002.001 — 12
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ANNEX III
REFERENCEMETHODSOFMEASUREMENT
ParameterMode of
expressionReference method of measurement
Rate of flow at the time
of samplingm3/s Flowmeter
Temperature ºC Thermometry
measured in situat the time of sampling
pH pH Electrometry
measured in situat the time of sampling without prior
treatment of the sample
Conductivity at 20 ºC µS cm−1Electrometry
Chlorides Cl mg/l Titration(Mohr's method)
Molecular absorptionspectrophotometry
Nitrates NO3mg/l Molecular absorptionspectrophotometry
Ammonia NH4mg/l Molecular absorptionspectrophotometry
Dissolved oxygenO2mg/l Winkler's method
Electrochemical method
BOD5O2mg/l Determination of dissolved oxygen before and after five-
day incubation at 20 ± 1 ºC in complete darkness. Addi-
tion of a nitrification inhibitor.
COD O2mg/l Potassium dichromate method
Total phosphorus P mg/l Molecular absorptionspectrophotometry
Surfactants reacting to
methylene blueSodium
lauryl
sulphate
eq. mg/lMolecular absorptionspectrophotometry
Total cadmium Cd mg/l Atomic absorptionspectrophotometry
Mercury Hg mg/l Flameless atomic absorptionspectrophotometry
Faecal coliforms / 100 ml — Culture at 44 ºC onanappropriate specific solid
medium (such as Tergitol lactose agar, Endo agar,
0,4 % Teepol broth) with or without filtrationan d
colony count. Samples must be diluted or, where
appropriate, concentrated in such a way as to contain
between 10 and 100 colonies. If necessary, identifica-
tionby gasification .
— Method of dilutionwith fermen tationinliquid
substrates inat least three tubes inthree dilution s.
Sub-culturing of the positive tubes on a confirmation
medium. Count according to CONS.ACTN (most
probable number). Incubation temperature: 44 ± 0,5 ºC.1977D0795 — EN — 05.06.2003 — 002.001 — 13
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ParameterMode of
expressionReference method of measurement
Total coliforms /100 ml — Culture at 37 ºC onanappropriate specific solid
medium (such as Tergitol lactose agar, Endo agar,
0,4 % Teepol broth) with or without filtrationan d
colony count. Samples must be diluted or, where
appropriate, concentrated in such a way as to contain
between 10 and 100 colonies. If necessary, identifica-
tionby gasification .
— Method of dilutionwith fermen tationinliquid
substrates inat least three tubes inthree dilution s.
Subculturing of the positive tubes on a confirmation
medium. Count according to CONS.ACTN (most
probable number). Incubation temperatures: 37 ºC
± 1 ºC.
Faecal streptococci /100 ml — Culture at 37 ºC onanappropriate solid medium (such
as sodium azide) with or without filtration and colony
count.
— Method of dilutioninsodium azide broth (Litsky).
Count according to CONS.ACTN (most probable
number).
Salmonella /1 l Concentration by filtration (on membrane or appropriate
filter). Innoculation (SIC! Inoculation) into pre-enriche-
ment (SIC! pre-enrichment) medium. Enrichment and
transfer into isolating gelose — identification.
Biological quality Pending Community-wide harmonization, Member States will use their
respective methods.1977D0795 — EN — 05.06.2003 — 002.001 — 14