1992L0066 — EN — 05.06.2003 — 002.001 — 1
This document is meant purely as a documentation tool and the institutions do not assume any liability for its contents
►B Council Directive 92/66/EEC
of 14 July 1992
introducing Community measures for the control of Newcastle disease
(OJ L 260, 5.9.1992, p. 1)
Amended by:
Official Journal
No page date
►M1Council Regulation (EC) No 806/2003 of 14 April 2003 L 122 1 16.5.2003
Amended by:
►A1Act of Accession of Austria, Sweden and Finland C241 21 29.8.1994
(adapted by Council Decision 95/1/EC, Euratom, ECSC) L 1 1 1.1.1995
▼B
Council Directive 92/66/EEC
of 14 July 1992
introducing Community measures for the control of Newcastle
disease
THE COUNCIL OF THE EUROPEAN COMMUNITIES,
Having regard to the Treaty establishing the European EconomicCommunity, and in particular Article 43 thereof,
Having regard to the proposal from the Commission (
1),
Having regard to the opinion of the European Parliament (2),
Having regard to the opinion of the Economic and Social
Committee (3),
Whereas poultry is listed in Annex II of the Treaty and whereas the
marketing of poultry constitutes an important source of revenue for
the agricultural population;
Whereas, it is necessary to establish at Community level the control
measures to be taken in the event of an outbreak of Newcastle disease
in order to ensure development of the poultry sector and contribute tothe protection of animal health in the Community;
Whereas minimum Community control measures should furthermore be
provided for against Newcastle disease to apply to certain otherspecies;
Whereas an outbreak of Newcastle disease can quickly take on
epizootic proportions, causing mortality and disturbances on a scaleliable to reduce sharply the profitability of farming or poultry as awhole;
Whereas action must be taken as soon as the presence of the disease is
suspected so that immediate and effective control measures can beimplemented when its presence is confirmed; whereas such measures
must be modulated by the competent authorities to take account of
whether or not a country carries out a prophylactic vaccinationprogramme in all or part of its territory;
Whereas it is necessary to prevent any spread of the disease as soon as
an outbreak occurs, by carefully monitoring movements of animals andthe use of products liable to be contaminated, and, where appropriate,by vaccination;
Whereas diagnosis of the disease must be carried out under the
auspices of responsible national laboratories, the coordination of whichmust be ensured by a Community reference laboratory;
Whereas it is necessary to make provision for the Member States which
vaccinate to draw up vaccination plans and to inform the Commissionand other Member States about those plans;
Whereas Article 4 of Council Decision 90/424/EEC of 26 June 1990 on
expenditure in the veterinary field (
4) applies in the event of the occur-
rence of Newcastle disease;
Whereas it is appropriate to confer upon the Commission the task of
taking the necessary applicatory measures,1992L0066 — EN — 05.06.2003 — 002.001 — 2
(1) OJ No C 146, 5. 6. 1991, p. 12.
(2) OJ No C 280, 28. 10. 1991, p. 174.
(3) OJ No C 339, 31. 12. 1991, p. 14.
(4) OJ No L 224, 18. 8. 1990, p. 19; amended by Decision 91/133/EEC (OJ No
L 66, 13. 3. 1991, p.18).
▼B
HAS ADOPTED THIS DIRECTIVE:
Article 1
Without prejudice to the Community provisions governing intra-
Community trade, this Directive defines the Community controlmeasures to be applied in the event of an outbreak of Newcastledisease in:
(a) poultry;
(b) racing pigeons and other birds kept in captivity.
This Directive shall not apply where Newcastle disease is detected in
wild birds living freely; however, in that case, the Member State
concerned shall inform the Commission of any measure it takes.
Article 2
For the purpose of this Directive, the definitions given in Article 2 of
Council Directive 90/539/EEC of 15 October 1990 on animal health
conditions governing intra-Community trade in, and imports from third
countries of, poultry and hatching eggs (
1) shall apply as appropriate.
The following definitions shall also apply:
(a) ‘infected poultry’ means any poultry:
— in which the presence of Newcastle disease has been officially
confirmed following an examination by an approved laboratory,
or
— in the case of second and subsequent outbreaks in which clin-
ical symptoms or post-mortem lesions consistent withNewcastle disease are present;
(b) ‘poultry suspected of being infected’ means any poultry showing
clinical signs or post-mortem lesions which are such that thepresence of Newcastle disease may reasonably be suspected;
(c) ‘poultry suspected of being contaminated’ means any poultry which
may have been directly or indirectly exposed to the Newcastle-disease virus;
(d) ‘swill’ means waste from kitchens, restaurants or, where appro-
priate, from industries using meat;
(e) ‘competent authority’ means the competent authority within the
meaning of Article 2 (6) of Directive 90/425/EEC (
2);
(f) ‘official veterinarian’ means the veterinarian designated by the
competent authority;
(g) ‘racing pigeon’ means any pigeon transported or intended for trans-
port from its pigeon house to be released so that it may freely fly
back to its pigeon house or to any other destination;
(h) ‘pigeon house’ means any installation used for keeping or breeding
racing pigeons.
Article 3
Member States shall ensure that there is compulsory and immediate
notification of the suspected presence of Newcastle disease to thecompetent authority.1992L0066 — EN — 05.06.2003 — 002.001 — 3
(1) OJ No L 303, 31. 10. 1990, p. 6; as last amended by Directive 91/496/EEC
(OJ No L 268, 24. 9. .1991, p. 56).
(2) Council Directive 90/425/EEC of 26 June 1990 concerning veterinary and
zootechnical checks applicable in intra-Community trade in certain live
animals and products with a view to the completion of the internal market
(OJ No L 224, 18. 8. 1990, p. 29); as last amended by Directive 91/496/EEC
(OJ No L 268, 24. 9. 1991, p. 56).
▼B
Article 4
1. When poultry in a holding are suspected of being infected or
contaminated with Newcastle disease, Member States shall ensure thatthe official veterinarian immediately activates official investigation
arrangements to confirm or rule out the presence of the disease and,
in particular, must take or have taken the samples necessary for labora-tory examination.
2. As soon as the suspected infection is notified, the competent
authority shall have the holding placed under official surveillance andshall in particular require that:
(a) a record be made of all categories of poultry on the holding
showing in respect of each of the categories the numbers of poultrywhich have died, which show clinical signs, and which show nosigns. The record shall be kept up-to-date to include birds born or
dying during the period in which there is a suspicion. The data in
the record shall be kept up-to-date and be produced on request, andmay be checked at each visit;
(b) all poultry on the holding are kept in their living quarters or
confined in some other place where they can be isolated and
without contact with other poultry;
(c) no poultry enter or leave the holding;
(d) all movement
— of persons, other animals and vehicles to or from the holding,
— of poultry meat or carcasses, or of animal feed, implements,
waste, droppings, manure litter or anything liable to transmitNewcastle disease be subject to authorization by the competentauthority;
(e) no eggs shall leave the holding with the exception of eggs sent
directly to an establishment approved for the manufacture and/orprocessing of egg products under Article 6 (1) of Directive 89/437/EEC (
1), and transported under an authorization which has
been granted by the competent authority. Such authorization must
meet the requirements laid down in Annex 1;
(f) appropriate means of disinfection be installed at the entrances and
exits of buildings housing poultry and of the holding itself;
(g) an epizootiological inquiry be carried out in accordance with
Article 7.
3. Until suchtime as th e official measures laid down in paragraph2
are enforced, the owner or keeper of any poultry in which disease is
suspected shall take all reasonable action to ensure compliance with
paragraph2, except for (g) th ereof.
4. The competent authority may apply any of the measures provided
for in paragraph 2 to other holdings should their location, their config-uration or contacts with the holding where the disease is suspected givereason to suspect possible contamination.
5. The measures referred to in paragraphs 1 and 2 shall not be with-
drawn until the suspicion of Newcastle disease has been ruled out bythe official veterinarian.
Article 5
1. Once the presence of Newcastle disease in poultry has been offi-
cially confirmed on a holding, the Member States shall ensure that the1992L0066 — EN — 05.06.2003 — 002.001 — 4
(1) Council Directive 89/437/EEC of 20 June 1989 on hygiene and health
problems affecting the production and the placing on the market of egg
products (OJ No L 272, 22. 7. 1989, p. 87); as amended by Directive 89/
662/EEC (OJ No L 395, 30. 12. 1989, p. 13).
▼B
competent authority requires, in addition to the measures listed in
Article 4 (2), the following measures to be untertaken:
(a) all poultry on the holding shall without delay be killed on the spot.
The poultry which have died or been killed and all eggs shall be
destroyed. These operations shall be carried out in a way which
minimizes the risk of spreading disease;
(b) any substance or waste, suchas animal feed, litter or manures
liable to be contaminated, shall be destroyed or treated appropri-ately. This treatment, carried out in accordance with the
instructions of the official veterinarian, shall ensure the destruction
of any Newcastle-disease virus present;
(c) where poultry from the holding have been slaughtered during the
presumed incubation period of disease the meat from those poultry
shall wherever possible be traced and destroyed;
(d) hatching eggs laid during the presumed incubation period which
have been moved from the holding shall be traced and destroyed;but poultry which have already hatched from the eggs shall beplaced under official surveillance; table eggs laid during the
presumed incubation period which have been moved from the
holding shall wherever possible be traced and destroyed, unlessthey have previously been properly disinfected;
(e) after carrying out operations listed under (a) and (b), the buildings
used for housing poultry, their surroundings, the vehicles used for
transport and all equipment likely to be contaminated shall be
cleaned and disinfected in accordance withth e provisions ofArticle 11;
(f) no poultry shall be reintroduced to the holding until at least 21
days after completion of operations provided for under (e);
(g) an epizootiological inquiry shall be carried out in accordance with
Article 7.
2. The competent authority may extend the measures provided for in
paragraph 1 to other neighbouring holdings should their location, theirconfiguration, or contact with the holding where the disease has beenconfirmed give reason to suspect possible contamination.
3. Where a strain of Newcastle-disease virus with an ICPI (intracer-
ebral pathogenicity index) greater than 0,7 and lower than 1,2 is
isolated in a flock of poultry that presents no clinical signs ofNewcastle disease and it has been demonstrated by the Communityreference laboratory referred to in Article 15 that the virus isolate inquestion derives from an atenuated live Newcastle-disease vaccine, thecompetent authority may grant a derogation from the requirements
under (a) to (f), of paragraph 1, provided that the holding concerned
is placed under official surveillance for 30 days and must require inparticular that:
— the provisions of Article 4 (2) (a), (b), (d), (e) and (f) are applied,
— no poultry may leave the holding except to be taken directly to a
slaughterhouse designated by the competent authority.
The competent authority responsible for this slaughterhouse must be
informed of the intention to send poultry there for slaughter and as
soon as the poultry arrive at the slaughterhouse they shall be kept and
slaughtered separately from other poultry.
4. Fresh meat from the poultry referred to in paragraph (3) of this
Article must carry the health mark provided for in Article 5 (1) ofDirective 91/494/EEC.
5. The provisions laid down in paragraph 3 shall be subject to a
review taking into account developments in scientific researchwithaview to adopting harmonized rules for the use of Newcastle-diseasevaccines in the Community.1992L0066 — EN — 05.06.2003 — 002.001 — 5
▼B
Article 6
In the case of holdings which consist of two or more separate flocks,
the competent authority may, in accordance with criteria set by the
Commission under the procedure laid down in Article 25, grant a dero-
gation from the requirements of Article 5 (1), for healthy flocks of aholding which is infected, provided that the official veterinarian hasconfirmed that the operations carried out there are such that the flocksare completely separate as regards housing, keeping and feeding, sothat the virus cannot spread from one flock to another.
Article 7
1. The epizootiological inquiry shall deal with:
— the length of time during which Newcastle disease may have
existed on the holding or in the pigeon house;
— the possible origin of the Newcastle disease on the holding or in the
pigeon house and the identification of other holdings or pigeonhouses in which there are poultry, pigeons or other birds kept incaptivity which may have become infected or contaminated from
the same source;
— the movement of persons, poultry, pigeons or other birds kept in
captivity or other animals, vehicles, eggs, meat and carcases and
any implement or substance likely to have carried Newcastle-disease virus to or from the holding or pigeon house in question.
2. In order to provide full coordination of all measures necessary to
ensure eradication of Newcastle disease as quickly as possible and forthe purpose of carrying out the epizootiological inquiry, a crisis unit
shall be established.
The general rules concerning national crisis units and Community crisis
units will be laid down by the Council, acting by a qualified majorityproposal from the Commission.
Article 8
1. Where the official veterinarian has reason to suspect that poultry
on any holding may have been contaminated as a result of the move-ment of persons, animals or vehicles or in any other way, that holdings
shall be placed under official control in accordance with paragraph 2.
2. The purpose of the official control shall be to detect immediately
any suspicion of Newcastle disease, count the poultry and monitor theirmovements and, where appropriate, to take the action provided for inparagraph3.
3. When a holding is subject to official control under paragraphs 1
and 2, the competent authority shall prohibit removal of poultry from
the holding other than for transport directly to a slaughterhouse under
official supervision for the purpose of immediate slaughter. Beforegranting such authorization, the official veterinarian must have carriedout a clinical examination of all the poultry to exclude presence ofNewcastle disease on the holding. The movement restrictions referred
to in this Article shall be imposed for a period of 21 days from the
latest date of potential contamination; however, such restrictions mustapply for a period of at least seven days.
4. Where it considers that conditions permit, the competent authority
may limit the measures provided for in this Article to a part of theholding and to the poultry contained therein, provided that the poultrythere have been housed, kept and fed completely separately be separate
staff.
5. Where the official veterinarian has reason to suspect racing
pigeons or any pigeon house of being contaminated by the Newcastle-disease virus, he shall take all appropriate steps to ensure that thepigeon house is subject to restrictions, including a ban on all movementof racing pigeons outside the pigeon house for 21 days.1992L0066 — EN — 05.06.2003 — 002.001 — 6
▼B
Article 9
1. Once the diagnosis of Newcastle disease has been officially
confirmed in poultry, the Member States shall ensure that the compe-tent authority establishes around the infected holding a protection zonebased on a minimum radius of three kilometres, itself contained in asurveillance zone based on a minimum radius of 10 kilometres. The
establishment of the zones must take account of geographical, adminis-
trative, ecological and epizootiological factors relating to Newcastledisease, and of monitoring facilities.
2. The measures applied in the protection zone shall include:
(a) the identification of all holdings having poultry within the zone;
(b) periodic visits to all the holdings having poultry, a clinical exami-
nation of those poultry including, if necessary, the collection of
samples for laboratory examination; a record of visits and findings
must be kept;
(c) the keeping of all poultry in their living quarters or some other
place where they can be isolated;
(d) the use of appropriate means of disinfection at the entrances and
exits of the holding;
(e) the control of movements or persons handling poultry, poultry
carcases and eggs and vehicles carrying poultry, carcases and eggswithin the zone; in general, transport of poultry shall be prohibited,
except for transit by major highways or railways;
(f) a prohibition on removing poultry and hatching eggs from the
holding on which they are kept unless the competent authority hasauthorized the transport;
(i) of poultry for immediate slaughter to a slaughterhouse prefer-
ably located in the infected area or, if that is not possible, to aslaughterhouse designated by the competent authority outsidethe infected area. The special health mark provided for in
Article 5 (1) of Directive 91/494/EEC (
1) must be applied to
this poultry meat;
(ii) of day-old chicks or ready-to-lay pullets to a holding within
the surveillance zone at which there are no other poultry.However, the Member States, that are not in a position to be
able to provide transport of day-old chicks or ready-to-lay
pullets to a holding situated within the surveillance zone, shallbe authorized, in accordance with the procedure laid down inArticle 25, to have the said chicks and pullets transported to aholding outside the surveillance zone. The holdings referred to
above must be placed under official control in accordance with
Article 8 (2);
(iii) of hatching eggs to a hatchery designated by the competent
authority; before dispatch, eggs and their packing must bedisinfected.
Movements allowed in (i), (ii) and (iii) shall be directly executed,
under official control. They shall be authorized only after the offi-
cial veterinarian has carried out a health inspection of the holding.The means of transport used must be cleaned and disinfectedbefore and after use;
(g) a prohibition on removing or spreading used litter or poultry
manure without authorization;
(h) the prohibition of fairs, markets, shows or other gatherings of
poultry or other birds.
3. The measures applied in the protection zone shall be maintained
for at least 21 days after the carrying out of preliminary cleaning anddisinfection operations on the infected holding in accordance with1992L0066 — EN — 05.06.2003 — 002.001 — 7
(1) Council Directive 91/494/EEC of 26 June 1991 on animal health conditions
governing intra-Community trade in and imports from third countries of
freshpoultrymeat (OJ No L 268, 24. 9. 1991, p. 35).
▼B
Article 11. The protection zone shall thereafter be part of the surveil-
lance zone.
4. The measures applied in the surveillance zone shall include:
(a) the identification of all holdings having poultry within the zone;
(b) the control of poultry and hatching egg movement within the zone;
(c) a prohibition on the movement of poultry out of the zone during
the first 15 days, except for movement directly to a slaughterhouseoutside the surveillance zone designated by the competentauthority. The special health mark provided for in Article 5 ofDirective 91/494/EEC must be applied to this poultry meat;
(d) a prohibition on the movement of hatching eggs out of the surveil-
lance zone unless to a hatchery designated by the competentauthority. Before dispatch the eggs and their packing must be disin-
fected;
(e) a prohibition on the movement of used litter or poultry manure out
of the zone;
(f) a prohibition of fairs, markets, shows or other gatherings of poultry
and other birds;
(g) without prejudice to the provisions of (a) and (b), the prohibition of
transport of poultry except for transit by major highways or rail-ways.
5. The measures applied in the surveillance zone shall be maintained
for at least 30 days after the carrying out of preliminary cleaning anddisinfection operations on the infected holding in accordance with
Article 11.
6. Where the zones are situated in the territory of more than one
Member State, the competent authorities of the Member Statesconcerned shall cooperate in establishing the areas described in para-graph 1. However, if necessary, the protection zone and thesurveillance zone shall be established by the procedure provided for in
Article 25.
7. Where the epizootiological enquiry referred to in Article 7
confirms that the outbreak is due to an infection where there is noevidenc of lateral spread, the size and duration of the protection andsurveillance zones may be reduced under the procedure laid down in
Article 25.
Article 10
Member States shall ensure that:
(a) the competent authority determines the arrangements allowing them
to trace the movement of eggs, poultry and birds kept in captivity;
(b) the owner or keeper of poultry and/or racing pigeons and/or birds
kept in captivity is required to supply the competent authority, inresponse to any request by that authority, with informationconcerning poultry and eggs entering or leaving his holding, aswell as information on the races or shows in which the racing
pigeons have taken part;
(c) all persons engaged in the transport or marketing of poultry, eggs,
racing, pigeons and birds kept in captivity are able to supply thecompetent authority with information concerning the movements
of poultry, eggs, racing pigeons and birds kept in captivity which
they have transported or marketed and to furnish all the detailsconcerning suchinformation.
Article 11
Member States shall ensure that:
(a) the disinfectants to be used and their concentrations are officially
approved by the competent authority;1992L0066 — EN — 05.06.2003 — 002.001 — 8
▼B
(b) the cleaning and disinfection operations are carried out under offi-
cial supervision, in accordance with:
(i) instructions given by the official veterinarian;
(ii) the procedure for cleaning and disinfecting an infected holding,
as laid down in Annex II.
Article 12
Collection of samples and laboratory testing to detect the presence of
Newcastle-disease virus shall be carried out in accordance with AnnexIII.
Article 13
Member States shall ensure that the competent authority takes all the
necessary measures for persons established in the protection and
surveillance zones to be informed of the restrictions in force and
make all necessary arrangements for the appropriate implementationof the measures in question.
Article 14
1. Member States shall ensure that, in each Member State there is
designated:
(a) a national laboratory at which facilities and expert personnel shall
be maintained to permit full antigenic and biological typing ofNewcastle-disease virus at all times and to confirm results obtained
in regional diagnostic laboratories;
(b) a national laboratory at which reagents for use in regional labora-
tories are tested;
(c) a national institute or laboratory at which vaccines allowed for
prohpylactic use in the country or stock-piled for emergency usemay be tested for efficacy, potency and purity.
2. The national laboratories listed in Annex IV shall be responsible
for coordinating standards and methods of diagnosis, use of reagents
and testing of vaccines.
3. The national laboratories listed in Annex IV shall be responsible
for coordinating the standards and diagnostic methods laid down ineach Newcastle-disease diagnostic laboratory within the Member State.To this end:
(a) they may provide diagnostic reagents to national laboratories;
(b) they shall control the quality of all diagnostic reagents used in that
Member State;
(c) they shall arrange comparative tests periodically;
(d) they shall hold isolates of Newcastle-disease virus from cases
confirmed in that Member State;
(e) they shall ensure the confirmation of positive results obtained in
regional diagnostic laboratories.
4. The national laboratories listed in Annex IV shall liaise with the
Community reference laboratory referred to in Article 15.
Article 15
The Community reference laboratory for Newcastle disease is
mentioned in Annex V. Without prejudice to the provisions of Decision90/424/EEC (
1), and in particular Article 28 thereof, the powers and
duties of the laboratory shall be those appearing in the said Annex.1992L0066 — EN — 05.06.2003 — 002.001 — 9
(1) OJ No L 224, 18. 8. 1990, p. 19.
▼B
Article 16
1. Member States shall ensure that:
(a) vaccination against Newcastle disease withvaccines auth orized by
the competent authority may be used for a prophylactic purpose or
to supplement the control measures carried out when the diseaseappears;
(b) the only vaccines allowed are those which have received a
marketing authorization from the competent authority of theMember State in which the vaccine is used.
2. Further criteria for using vaccines against Newcastle disease may
be established in accordance with the procedure laid down in Article25.
Article 17
1. A Member State in which voluntary or compulsory prophylactic
vaccination against Newcastle disease is carried out shall inform the
Commission and the other Member States.
2. The information given in accordance with paragraph 1 must
specify:
— the characteristics and composition of the vaccine to be used;
— the procedures for supervision of the distribution, storage and use of
vaccines;
— the species and categories of poultry which may or shall be subject
to vaccination;
— the areas in which vaccination may or shall be carried out;— the reasons for which vaccination is carried out.
3. Member States may provide for the establishment of a vaccina-
tion programme for racing pigeons. Where this is the case, they mustnotify the Commission. Without prejudice to such a programme, theMember States shall ensure that the organizers of races and shows
take the necessary steps so that only racing pigeons which have been
vaccinated against Newcastle disease may be entered in races andshows.
4. The detailed rules for applying this Article, in particular with
respect to the criteria to be adopted, and any derogations that may begranted taking into account the health status of the Member States shallbe fixed in accordance withth e procedure laid down in Article 25.
Article 18
1. Member States shall ensure that, when Newcastle disease has
been confirmed, the competent authority, in order to supplement the
other control measures provided for in this Regulation, is able to
specify a territorial area and period in which the prompt and systematicvaccination (emergency vaccination) of designated species of poultryshall be carried out under official control. A Member State applyingemergency vaccination shall inform the Commission and other MemberStates within the framework of the standing veterinary committee, set
up by Decision 68/361/EEC (
1), about the Newcastle-disease situation
and emergency vaccination programme.
2. In the case referred to in paragraph 1, the vaccination or re-vacci-
nation of poultry on the holding subject to the restrictions referred to in
Article 4 is prohibited.
3. In the case referred to in paragraph 1:
(a) the designated species of poultry shall be vaccinated as soon as
possible;
(b) all poultry of designated species born on or transferred to a holding
inside the vaccination area must be or have been vaccinated;1992L0066 — EN — 05.06.2003 — 002.001 — 10
(1) OJ No L 255, 18. 10. 1968, p. 23.
▼B
(c) throughout the vaccination operation provided for in paragraph 1
all poultry of designated species kept on holdings inside the vacci-nation area must remain there except:
— day-old chicks moved to a holding inside the vaccination area
where they shall be vaccinated;
— poultry moved direct to a slaughterhouse for immediate
slaughter. If the slaughterhouse is located outside the vaccina-tion area the movement of poultry shall be permitted only after
the official veterinarian has carried out a health inspection of
the holding;
(d) when the vaccination operations provided for in (a) have been
completed movements out of the vaccination area may be author-ized for:
— day-old chicks intended for meat production may be moved to a
holding where they shall be vaccinated; the holding in questionmust be kept under surveillance until the poultry which have
been moved have been slaughtered;
— poultry vaccinated more than 21 days previously and intended
for immediate slaughter;
— hatching eggs which originate from breeding poultry vaccinated
at least 21 days previously; the eggs and their packing must be
disinfected before movement.
4. The measures provided for in (b) and (d) of paragraph 3 shall be
applied for a period of three months following completion of vaccina-
tion operations provided for in paragraph1; th ey may be extended forone or more additional periods of three months.
5. By way of derogation from (a) and (b) of paragraph3, th e compe-
tent authorities may exempt certain flocks of particular scientific value
from systematic vaccination, provided that all necessary steps are takenby the competent authority to ensure protection of their health and tosubject them to periodic serological checks.
6. The Commission shall follow the development of the disease
situation and if necessary it may, in accordance withth e procedurelaid down in Article 25, take a decision in particular in relation tocontrol of movement and vaccination.
Article 19
1. Where carrier pigeons or birds kept in captivity are suspected of
being infected with Newcastle disease, Member States shall ensure that
the official veterinarian immediately starts the official investigation
procedures for confirming or notifying the presence of the disease; inparticular, he shall take adequate samples for laboratory examinationor see that they are taken.
2. As soon as it has been notified of the suspicion, the competent
authority shall place the holding or pigeon house under official surveil-lance and order that no pigeon or bird kept in captivity, and nothinglikely to transmit Newcastle disease, may leave the pigeon house orholding.
3. The measures provided for in paragraphs 1 and 2 shall not be
withdrawn until the suspicion of Newcastle disease has been ruled outby the official veterinarian.
4. As soon as the presence of Newcastle disease has been officially
confirmed, the competent authority shall, inter alia , order:
(a) application of the control and eradication measures provided for in
Article 5 (1) (a), (b), (e) and (f) to the carrier pigeons or birds keptin captivity and pigeon houses infected with Newcastle disease; or
(b) at least
(i) a ban on movement of the pigeons or birds kept in captivity
outside the pigeon house or holding for at least 60 days afterthe clinical signs of Newcastle disease have disappeared;1992L0066 — EN — 05.06.2003 — 002.001 — 11
▼B
(ii) destruction or treatment of any matter or waste likely to be
contaminated. Treatment must guarantee the destruction ofany Newcastle-disease virus present and all waste that has
accumulated during the 60-day period referred to in (i);
(c) an epizootiological inquiry in accordance withArticle 7.
5. To the extent that it is required for the proper application of the
provisions laid down in this Article, the Member States shall furnish to
the Commission, within the Standing Veterinary Committee, informa-
tion on the situation of the disease and the control measures appliedin accordance withth e model appearing in Annex VI.
Article 20
1. The use of swill originating from means of international transport,
such as ships, land vehicles or aircraft, shall be prohibited for thefeeding of poultry; suchswill sh all be collected and destroyed underofficial supervision.
2. The use of swill other than as defined in paragraph 1, or poultry
scraps, may be authorized for the feeding of poultry only after a heat-treatment in appropriate facilities ensuring that the disease is not trans-mitted and the Newcastle-disease virus is destroyed.
3. If necessary, the Commission shall, in accordance with the proce-
dure laid down in Article 25, lay down the detailed rules for the
implementation of paragraph2.
Article 21
1. EachMember State sh all draw up a contigency plan, specifying
the national measures to be implemented in the event of an outbreakof Newcastle disease.
This plan must allow access to facilities, equipment, personnel and all
other appropriate materials necessary for the rapid and efficient eradi-cation of the outbreak. It must give a precise indication of the vaccine
requirements which each Member State deems necessary for emer-
gency vaccination.
2. The criteria to be applied for drawing up the plan are laid down
in Annex VII.
3. Plans drawn up in accordance withth e criteria listed in Annex
VII shall be submitted to the Commission not later than six monthsafter this Directive is brought into application.
4. The Commission shall examine the plans in order to determine
whether they permit the desired objective to be attained and shall
suggest to the Member State concerned any amendments required inparticular to ensure that they are compatible with those of the otherMember States.
The Commission shall approve the plans, if necessary amended, in
accordance withth e procedure laid down in Article 25.
The plans may subsequently be amended or supplemented, in accor-
dance withth e same procedure, to take into account developments inthe situation.
Article 22
Commission experts may, in collaboration with the competent authori-
ties, and insofar as is necessary to ensure uniform application of thisDirective, make on-the-spot checks. In order to do this, they may checka representative percentage of establishments to see whether the
competent authorities are checking that these establishments are
fulfilling the requirements of this Directive. The Commission shallinform the Member States of the result of the checks carried out.
A Member State in whose territory a check is being carried out shall
give all the necessary assistance to the experts in carrying out theirduties.1992L0066 — EN — 05.06.2003 — 002.001 — 12
▼B
The general provisions for implementing this Article shall be deter-
mined in accordance withth e procedure laid down in Article 25.
Article 23
The detailed conditions governing the Community's financial contribu-
tion to the measures connected with the application of this Directive
are laid down in Decision 90/424/EEC.
Article 24
The Annexes shall be amended, as and when required, by the Council
acting by a qualified majority acting on a proposal from the Commis-
sion, in particular in order to take into account developments inresearchand in diagnostic procedures.
Article 25
1. The Commission shall be assisted by the Standing Committee on
the Food Chain and Animal Health set up pursuant to Article 58 ofRegulation (EC) No 178/2002 (
1).
2. Where reference is made to this Article, Articles 5 and 7 of Deci-
sion 1999/468/EC (2) shall apply.
The period laid down in Article 5(6) of Decision 1999/468/EC shall be
set at three months.
3. The Committee shall adopt its Rules of Procedure.
Article 26
1. Member States shall bring into force the laws, regulations and
administrative provisions necessary to comply withth is Directivebefore 1 October 1993. They shall forthwith inform the Commissionthereof.
When Member States adopt these measures, they shall contain a refer-
ence to this Directive or shall be accompanied by such reference on the
occasion of their official publication. The methods of making such areference shall be laid dwon by the Member States.
2. Member States shall communicate to the Commission the texts of
the provisions of national law which they adopt in the field governedby this Directive.
Article 27
This Directive is addressed to the Member States.1992L0066 — EN — 05.06.2003 — 002.001 — 13
(1) OJ L 31, 1.2.2002, p. 1.
(2) OJ L 184, 17.7.1999, p. 23.▼M1
▼B
▼B
ANNEX I
AUTHORIZATION TO REMOVE EGGS FROM A HOLDING SUBJECT
TO THE CONDITIONS OF ARTICLE 4 (2) (e) OF THIS DIRECTIVE
The authorization issued by the competent authority to transport eggs from a
suspect holding subject to the provisions of Article 4 (2) (e) to an establishment
approved for the manufacture and processing of egg products in accordance with
the provisions of Article 6 (1) of Directive 89/437/EEC, hereinafter called the
designated establishment, must meet the following conditions:
1. in order to be allowed to be removed from a suspect undertaking, eggs must:
(a) comply with the requirements laid down in Chapter IV of the Annex to
Directive 89/437/EEC;
(b) be sent directly from the suspect undertaking to the designated establish-
ment; eachconsignment must be sealed before dispatchby th e official
veterinarian of the suspect holding and must remain sealed throughout
transport to the designated establishment;
2. the official veterinarian of the suspect undertaking shall inform the compe-
tent authority of the designated establishment of his intention of sending
eggs to it;
3. the competent authority responsible for the designated establishment shall
ensure that:
(a) eggs referred to in 1 (b) will be kept isolated from other eggs from the
time they arrive until they are processed;
(b) the shells of such eggs shall be regarded as high-risk material in accor-
dance withArticle 2 (2) of Directive 90/667/EEC (1) and shall be dealt
with in accordance with the requirements of Chapter II of that Directive;
(c) the packaging material, the vehicles used to transport eggs referred to in
1 (b) and all premises with which the eggs come into contact are cleaned
and disinfected in sucha way as to destroy all Newcastle disease virus;
(d) the official veterinarian of the suspect holding shall be informed of all
consignments of processed eggs.1992L0066 — EN — 05.06.2003 — 002.001 — 14
(1) Council Directive 90/667/EEC of 27 November 1990 laying down the veterinary rules
for the elimination and processing of animal waste, for its placing on the market andfor the prevention of pathogens in feedstuffs of animal or fish origin, and amending
Directive 90/425/EEC (OJ No L 363, 27. 12. 1990, p. 51).
▼B
ANNEX II
PROCEDURE FOR CLEANING AND DISINFECTING AN INFECTED
HOLDING
I.Preliminary cleaning and disinfecting
(a) As soon as the carcases of the poultry have been removed for disposal,
those parts of the premises in which the poultry was housed and any
parts of other buildings, yards etc. contaminated during slaughter or
post-mortem examination should be sprayed with disinfectants approved
for use in accordance withArticle 11 of th is Directive.
(b) Any tissue of poultry or eggs which could have contaminated buildings,
yards, utensils etc. should be carefully collected and disposed of with the
carcases.
(c) The used disinfectant must remain on the surface for at least 24 hours.
II.Final cleaning and disinfection
(a) Grease and dirt should be removed from all surfaces by the application
of a degreasing agent and washed with water.
(b) After washing with water as described in (a), further spraying with disin-
fectant should be applied.
(c) After seven days the premises should be treated with a degreasing agent,
rinsed withcold water, sprayed withdisinfectant and rinsed again with
water.
(d) Used litter and manure must be treated by a method capable of killing
the virus. This method must comprise at least one of the following prac-
tices:
(i) incineration or steam treatment at a temperature of 70 ºC;
(ii) burying deep enoughto prevent access by vermin and wild birds;(iii) stacking and dampening (if necessary to facilitate fermentation),
covering to keep in the heat so that a temperature of 20 ºC is
attained and leaving covered for 42 days so as to prevent access
by vermin and wild birds.1992L0066 — EN — 05.06.2003 — 002.001 — 15
▼B
ANNEX III
DIAGNOSTIC PROCEDURES FOR THE CONFIRMATION AND
DIFFERENTIAL DIAGNOSIS OF NEWCASTLE DISEASE
The following procedures for the isolation and characterization of Newcastle-
disease viruses should be regarded as guidelines and the minima to be applied
in the diagnosis of the disease.
The virus responsible for Newcastle disease is the prototype virus of the Para-
myxoviridae. At present, there are nine serologically distinguishable groups of
Avian Paramyxoviruses, which have been designated PMV-1 to PMV-9. All
Newcastle disease viruses are placed in the PMV-1 group. For the purpose of
the diagnostic procedures for the confirmation and differential diagnosis of
Newcastle disease the following definition shall apply:
‘Newcastle disease’ means an infection of poultry caused by any avian strain of
the paramyxovirus 1 with an intracerebral pathogenicity index (ICPI) in day-old
chicks greater than 0,7.
CHAPTER 1
Sampling and treatment of samples
1.Samples
Cloacal swabs (or faeces) and tracheal swabs from sick birds; faeces or
intestinal contents, brain tissue, trachea, lungs, liver, spleen and other
obviously affected organs from recently dead birds.
2.Treatment of samples
The organs and tissues listed in paragraph 1 may be pooled, but separate
treatment of faecal material is essential. Swabs should be placed in sufficient
antibiotic medium to ensure full immersion. Faeces samples and organs
should be homogenized (in an enclosed blender or using a pestle and mortar
and sterile sand) in antibiotic medium and made to 10-20 % w/v suspensions
in the medium. The suspensions should be left for about two hours at
ambient temperature (or longer periods at 4 ºC) and then clarified by centri-
fugation (e.g. 800 to 1000 g for 10 minutes).
3.Antibiotic medium
Different laboratories have used various formulations of antibiotic medium
withsuccess and laboratories referred to in Annex II will be able to offer
advice for a particular country. Highconcentrations of antibiotics are
required for faeces samples and a typical mixture is: 10000 units/ml peni-
cillin, 10 mg/ml streptomycin, 0,25 mg/ml gentamycin and 5 000 units/mlmycostatin in phosphate buffered saline (PBS) These levels can be reduced
up to five-fold for tissues and tracheal swabs. For control of Chlamydia
organisms, 50 mg/ml oxytetracycline may be added. It is imperative when
making the medium that the pH is checked after the addition of the antibio-
tics and readjusted to pH 7,0-7,4.
CHAPTER 2
Virus isolation
Virus isolation in embryonated fowls' eggs
The clarified supernatent fluid should be inoculated in 0,1-0,2 ml amounts into
the allantoic cavity of each of a minimum of four embryonated, fowls' eggs
which have been incubated for 8 to 10 days. Ideally, these eggs should be
obtained from a specific pathogen-free flock, but when this is impracticable it
is acceptable to use eggs obtained from a flock shown to be free of antibodies
to Newcastle-disease virus. The inoculated eggs are held at 37 ºC and candled
daily. Eggs withdead or dying embryos as th ey arise, and all remaining eggs six
days after inoculation should be chilled to 4 ºC and the allantoic-amniotic fluids
tested for haemagglutination activity. If no haemagglutination is detected the
above procedure is repeated using undiluted allantoic/amniotic fluid as
inoculum.
When haemagglutination is detected the presence of bacteria should be excluded
by culture. If bacteria are present the fluids may be passed through a 450 nm
membrane filter, further antibiotics added and inoculated into embryonated
eggs as above.1992L0066 — EN — 05.06.2003 — 002.001 — 16
▼B
CHAPTER 3
Differential diagnosis
1.Preliminary differentiation
It is intended that all haemagglutinating viruses should be submitted to the
national laboratory referred to in Annex II for full identification, characteri-
zation and pathogenicity tests. However, it is important that interim control
measures for Newcastle disease aimed at limiting the spread of the virus
should be implemented as soon as possible and regional laboratories should
be able to identify the presence of Newcastle disease virus. The haemagglu-
tinating fluids should, therefore, be used in an haemagglutination inhibition
test as described in Chapters 5 and 6. Positive inhibition 1. e. 24, or more,
withth e Newcastle disease virus specific polyclonal antiserum of titre known
to be at least 29would serve as preliminary identification enabling the impo-
sition of interim control measures.
2.Confirmatory identification
The national laboratory should undertake full differential diagnosis of any
haemagglutinating agent. Confirmation of Newcastle-disease virus would
again be by inhibition in haemagglutination inhibition tests with monospe-
cific chicken antisera. Intracerebral pathogenicity index tests as described in
Chapter 7 should be carried out on all positive isolates. Pathogenicity indices
of greater than 0,7 indicate the presence of virus requiring the full implemen-
tation of control measures.
Recent developments in typing Newcastle-disease viruses, particularly mono-
clonal antibody techniques, has enabled grouping of the strains and isolates.
In particular, some monoclonal antibodies are available which are specific for
the vaccinal strains used on the territory of the Community and can be
employed in simple haemagglutination inhibition tests.
Since live vaccine strains may often be isolated from sampled poultry the
advantage of their rapid identification at the national laboratory referred to
in Annex II is obvious. Suchmonoclonal antibodies sh ould be obtained by
the Community reference laboratory as referred to in Article 14 and supplied
to the national laboratories to enable confirmation of the isolation of vaccinal
viruses.
The national laboratories should submit all haemagglutinating agents to the
Community reference laboratory.
3.Further typing and characterization of isolates
The Community reference laboratory should receive all haemagglutinating
viruses from the national laboratories for further antigenic and genetic studies
to enable a greater understanding of the epizootiology of the disease(s)
within the Community in keeping with the functions and duties of the refer-
ence laboratory.
CHAPTER 4
Rapid tests for detection of Newcastle-disease virus and antibodies
Rapid tests for the detection of Newcastle disease virus in vaccinated birds and
the detection of antibodies in unvaccinated birds are outlined below:
1.Detection of Newcastle disease virus
Several rapid test that directly detect Newcastle-disease antigens have been
employed in the diagnosis of infections in vaccinated birds. Those most
commonly used to date are fluorescent antibody tests on longitudinal sections
of the trachea and peroxidase antibody tests on the brain. There seems no
reason to doubt that other direct antigen detection tests could be applied to
Newcastle-disease virus infections.
The drawback to such tests is that it is impracticable to examine all the
potential sites of replication of Newcastle-disease virus in the vaccinated
birds. So that, for example, absence of evidence of virus in the trachea does
not preclude virus replication in the gut. No direct detection method is
recommended for routine use in the diagnosis of Newcastle disease, although
in specific circumstances suchtests may h ave a useful role.
2.Detection of antibodies in unvaccinated birds
The majority of laboratories involved in Newcastle disease diagnosis are
familiar with the haemagglutination inhibition test and the recommendation1992L0066 — EN — 05.06.2003 — 002.001 — 17
▼B
described below relate to this test for the measurement of antibodies to the
virus. However, enzyme-linked immunosorbent assays (Elisa) may be
successfully used to detect antibodies to the virus. It is suggested that if there
is a wish to employ an Elisa test at regional laboratory level the test should
be monitored by the national laboratory referred to in Annex II.
(a) Samples
Blood samples should be taken from all birds if the flock size is less than
20 and from 20 birds from larger flocks (this will give a 99 % probability
of detecting at least one positive serum if 25 % or more of the flock is
positive, regardless of flock size). The blood should be allowed to clot
and serum removed for testing.
(b) Examination for antibodies
Individual serum samples should be tested for their ability to inhibit
Newcastle disease virus haemagglutinating antigen in standard haemag-
glutination inhibition tests as defined in Chapter 6.
There is some debate as to whether 4 or 8 haemagglutinin units should
be used for the HI test. It would appear that either is valid and the choice
should be left to the discretion of the national laboratories. However, the
antigen used will affect the level at which a serum is considered positive:
for 4 HAU a positive serum is any showing a titre of 24or greater and
for 8 HAU a positive serum is any showing a titre of 23or greater.
CHAPTER 5
Haemagglutination (HA) test
Reagents
1. Isotonic saline buffered with phosphate (PBS) (0,05 M) to pH 7,0-7,4.
2. Red blood cells (RBC) taken and pooled from a minimum of three specific
pathogen free chickens (if not available blood may be taken from birds regu-
larly monitored and shown to be free of NDV antibodies) into an equal
volume of Alsever's solution. Cells should be washed three times in PBS
before use. For the test a 1 % suspension (packed cell v/v) in PBS is recom-
mended.
3. NDV strain Ulster 2C is recommended for use as standard antigen.
Procedure
(a) Dispense 0,025 ml PBS into eachwell of a plastic microtitre plate (V-
bottomed wells should be used).
(b) Place 0,025 ml of virus suspension (i.e. allantoic fluid) in the first well.
(c) Use a microtitration diluter to make two-fold dilutions (1:2 to 1:4096) of
virus across the plate.
(d) Dispense a further 0,025 ml of PBS to each well.
(e) Add 0,025 ml of 1 % red blood cells to eachwell.
(f) Mix by tapping gently and place at 4 ºC.
(g) Plates are read 30 — 40 minutes later when controls are settled. Reading is
done by tilting the plate and observing the presence or absence of tear-
shaped streaming of the RBCs. Wells with no HA should flow at the same
rate as the control cells with no virus.
(h) The HA titre is the highest dilution that causes agglutination of the RBCs.
That dilution may be regarded as containing one HA unit (HAU). A more
accurate method for determining the HA titre is to do HA tests on virus
from a close range of initial dilutions i.e. 1:3, 1:4, 1:5, 1:6 etc. This is
recommended for the accurate preparation of antigen for haemagglutination
inhibition tests (see Chapter 6).
CHAPTER 6
Haemagglutination inhibition (HI) test
Reagents (see Chapter 5)
(a) Phosphate buffered saline (PBS)
(b) Virus-containing allantoic fluid diluted withPBS to contain 4 or 8 HAU per
0,025 ml.
(c) 1 % chicken RBCs.1992L0066 — EN — 05.06.2003 — 002.001 — 18
▼B
(d) Negative control chicken serum.
(e) Positive control serum.
Procedure
(a) Dispense 0,025 ml PBS into all wells of a plastic microtitre plate (withV-
bottomed wells).
(b) Place 0,025 ml of serum into first well of plate.
(c) Use microtitration diluter to make two-fold dilutions of serum across plate.
(d) Add 0,025 ml of diluted allantoic fluid containing 4 or 8 HAU.
(e) Mix by tapping and place plate at 4 ºC for a minimum of 60 minutes or
room temperature for a minimum of 30 minutes.
(f) Add 0,025 ml 1 % RBCs to all wells.
(g) Mix by gentle tapping and place at 4 ºC.
(h) Plates are read after 30-40 minutes when control RBCs are settled. This is
done by tilting and observing the presence or absence of tear-shaped
streaming at the same rate as control wells containing RBCs (0,025 ml)
and PBCs (0,05 ml) only.
(i) The HI titre is the highest dilution of antiserum causing complete inhibition
of 4 or 8 units of virus (an HA titration to confirm the presence of the
required HAU should be included in each test).
(j) The validity of the results is dependent on obtaining a titre of less than 23
for 4 HAU or 22for 8 HAU withth e negative control serum and a titre of
within one dilution of the known titre of the positive control serum.
CHAPTER 7
Intracerebral pathogenicity index test
1. Infective freshly harvested allantoic fluid (HA titre must be greater than 24is
diluted 1:10 in sterile isotonic saline (anti-bodies must not be used).
2. 0,05 ml of the diluted virus is injected intracerebrally into each of 10 one-day
old chicks (i.e. 24 hours; 40 hours after hatching). The chicks should be
hatched from eggs obtained from a specific pathogen-free flock.
3. The birds are examined at intervals of 24 hours for eight days.4. At eachobservation eachbird is scored: 0 = normal; 1 = sick; 2 = dead.5. The index is calculated as shown in the following example:
Clinical signsDay after inoculation
(number of birds)
1 2 3 4 5 6 7 8 Total Score
normal 10 4 0 0 0 0 0 0 14 × 0 = 0
sick 0 6 10 4 0 0 0 0 20 × 1 = 20
dead 0 0 0 6 10 10 10 10 46 × 2 = 92
Total = 112
Index is a mean score per bird per observation = 112/80 = 1,4
CHAPTER 8
Assessment of plaque-forming ability
1. It is usually best to use a dilution range of virus to ensure that an optimum
number of plaques are present on the plate. Ten-fold dilutions up to 10−7in
PBS should be sufficient.
2. Confluent monolayers of chick embryo cells or a suitable cell line (Madin-
Darby bovine kidney for example) are prepared in 5 cm diameter Petri
dishes.
3. 0,2 ml of eachvirus dilution is added to eachof two Petri dish es and th e
virus allowed to absorb for 30 minutes.1992L0066 — EN — 05.06.2003 — 002.001 — 19
▼B
4. After washing three times with PBS the infected cells are overlaid with the
relevant medium containing 1 % w/v agar and either 0,01 mg/ml trypsin or
no trypsin. It is important that no serum is added to the overlay medium.
5. After 72 hours, incubation at 37 ºC the plaques should be of sufficient size.
They are best seen by removing the agar overlay and staining the cell mono-
layer withcrystal violet (0,5 % w/v) in 25 % v/v eth anol.
6. All viruses should give clear plaques when incubated in the presence of
trypsin in the overlay. When trypsin is absent from the overlay only viruses
virulent for chickens will produce plaques.1992L0066 — EN — 05.06.2003 — 002.001 — 20
▼B
ANNEX IV
LIST OF NATIONAL NEWCASTLE DISEASE LABORATORIES
BELGIUM Institut National de Recherches Vétérinaires
Groeselenberg 99
B-1180 Bruxelles
DENMARK National Veterinary Laboratory
Poultry Disease Division
Hangøvej 2
DK-8200 Aarhus N.
FEDERAL REPUBLIC OF
GERMANYBundesforschungsanstalt für Viruskrankheiten der Tiere
Anstaltsteil Riems (Friedrich-Löffler-Institut)
D-O-2201 Insel Riems
FRANCE Centre national d'études vétérinaires et alimentaires, Laboratoire
central de recherches agricoles et porcines
BP 53
F-22440 Ploufragan
GREECE Ινστιτούτο Λοιµωδών και Παρασιτικών Νοσηµάτων
Νεαπόλεως 25
Αγ. Παρασκευή —Αθήνα, Ελλάδα
IRELAND Veterinary ResearchLaboratory
Abbotstown
Castleknock, IRL-Dublin 15
ITALY Istituto Zooprofilattico Sperimentale di Padova
Via G. Orus n. 2
I-35100 Padova
LUXEMBOURG Institut National de Recherches Vétérinaires
Groeselenberg 99
B-1180 Bruxelles
NETHERLANDS Centraal Diergeneeskundig Instituut
Vestiging Virologie
Houtribweg 39
NL-8221 RA Lelystad
PORTUGAL Laboratório Nacional de Investigação Veterinaria (LNIV)
Estrada de Benfica 701
1500 Lisboa
SPAIN Laboratório de Sanidad y Producción Animal
Zona Franca, Circunvalación — Tramo 6
Esquina Calle 3
E-08004 Barcelona
UNITED KINGDOM Central Veterinary Laboratory
New Haw, Weybridge
GB-Surrey KT15 3NB
AUSTRIA Bundesanstalt für Virusseuchenbekämpfung bei Haustieren,
Wien-Hetzendorf
FINLAND Eläinlääkintä- ja elintarvikelaitos, Helsinki — Anstalten för
veterinärmedicin ochlivsmedel, Helsingfors
SWEDEN Statens veterinärmedicinska anstalt, Uppsala1992L0066 — EN — 05.06.2003 — 002.001 — 21
▼A1
▼B
ANNEX V
COMMUNITY REFERENCE LABORATORY FOR NEWCASTLE
DISEASE
Name of Laboratory:
Central Veterinary Laboratory
New Haw
Weybridge
Surrey KT15 3NBUnited Kingdom
The functions and duties of the Community reference laboratory for Newcastle
disease shall be:
1. to coordinate, in consultation with the Community Commission, the methods
employed in the Member States for diagnosing Newcastle disease. Specifi-
cally by:
(a) typing, storing and supplying strains of Newcastle-disease virus for sero-
logical tests and the preparation of antisera;
(b) supplying standard sera and other reference reagents to the national refer-
ence laboratories in order to standardize the tests and reagents used in the
Member States;
(c) building up and retaining a collection of Newcastle-disease virus strains
and isolates;
(d) organizing periodical comparative tests of diagnostic procedures at
Community level;
(e) collecting and collating data and information on the methods of diagnosis
used and the results of tests carried out in the Community;
(f) characterizing isolates of Newcastle disease viruses by the most up-to-
date methods available to allow greater understanding of the epizoo-
tiology of Newcastle disease;
(g) keeping abreast of developments in Newcastle disease surveillance,
epizootiology and prevention throughout the world;
(h) retaining expertise on Newcastle disease virus and other pertinent viruses
to enable rapid differential diagnosis;
(i) acquiring a thorough knowledge of the preparation and use of the
products of veterinary immunology used to eradicate and control
Newcastle disease;
2. to actively assist in the diagnosis of Newcastle disease outbreaks in Member
States by receiving virus isolates for confirmatory diagnosis, characterization
and epizootiological studies;
3. to facilitate the training or retraining of experts in laboratory diagnosis with a
view to the harmonization of techniques throughout the Community.1992L0066 — EN — 05.06.2003 — 002.001 — 22
▼B
ANNEX VI
1992L0066 — EN — 05.06.2003 — 002.001 — 23
▼B
ANNEX VII
MINIMUM CRITERIA FOR CONTIGENCY PLANS
Contingency plans shall meet at least the following criteria:
1. the establishment of a crisis centre on a national level, which shall coordi-
nate all control measures in the Member State concerned;
2. a list shall be provided of local disease control centres with adequate facil-
ities to coordinate the disease control measures at a local level;
3. detailed information shall be given about the staff involved in control
measures, their skills and their responsibilities;
4. eachlocal disease control centre must be able to contact rapidly persons or
organizations which are directly or indirectly involved in an outbreak;
5. equipment and materials shall be available to carry out the disease control
measures properly;
6. detailed instructions shall be provided on action to be taken on suspicion
and confirmation of infection or contamination, including proposed means
of disposal of carcases;
7. training programmes shall be established to maintain and develop skills in
field and administrative procedures;
8. diagnostic laboratories must have facilities for post-mortem examination,
the necessary capacity for serology, histology etc., and must maintain the
skills for rapid diagnosis. Arrangements must be made for rapid transporta-
tion of samples;
9. details shall be provided of the quantity of Newcastle disease vaccine esti-
mated to be required in the event of a reinstatement of emergency
vaccination;
10. provisions shall be made to ensure the legal powers necessary for the imple-
mentation of the contingency plans.1992L0066 — EN — 05.06.2003 — 002.001 — 24