1977D0795—EN—01.01.199 5—001.001—1
This document is meant purely as a documentation tool and the institutions do not assume any liability for its contents
►B COUNCILDECISION
of12December 1977
establishingacommon procedure for theexchange ofinformationonthe qualityofsurface fresh
waterintheCommunity
(77/795/EEC)
(OJ L 334, 24.12.1977, p. 29)
Amended by:
Official Journal
No page date
►M1Council Decision of 19 October 1981 (81/856/EEC) L 319 17 7.11.1981
►M2Commission Decision of 24 July 1984 (84/422/EEC) L 237 15 5.9.1984
►M3Council Decision of 24 November 1986 (86/574/EEC) L 335 44 28.11.1986
►M4Commission Decision of 14 December 1989 (90/2/EEC) L 1 20 4.1.1990
Amended by:
►A1Act of Accession of Greece L 291 17 19.11.1979
►A2Act of Accession of Spain and Portugal L 302 23 15.11.1985
►A3Act of Accession of Austria, Sweden and Finland C 241 21 29.8.1994(adapted by Council Decision 95/1/EC, Euratom, ECSC) L 1 1 1.1.1995
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COUNCILDECISION
of12December 1977
establishing a common procedure for the exchange of information
onthequalityofsurfacefreshwaterintheCommunity
(77/795/EEC)
THE COUNCIL OF THE EUROPEAN COMMUNITIES,
Having regard to the Treaty establishing the European Economic
Community, and in particular Article 235 thereof,
Having regard to the proposal from the Commission,Having regard to the opinion of the European Parliament(
1),
Having regard to the opinion of the Economic and Social
Committee(2),
Whereasthe 1973(3) and 1977(4) programmesof action of the
European Communitieson the environment provide for the introductionof a procedure for the exchange of information between the pollutionsurveillance and monitoring networks;
Whereas such a procedure is necessary to determine the pollution
levelsof the riversin the Community and cons equently to lay down
guidelines for the control of pollution and nuisances, which is one ofthe Community'sobjectivesin res pect of the improvement of livingconditionsand the harmoniousdevelopment of economic activitiesthroughout the Community; whereasno provis ion ismade in the Treatyfor the specific powers required for this purpose;
Whereass uch an exchange of information on pollution levelsisone of
the meansof monitoring the long-term trendsand the improvements
resulting from the application of current national and Community rules;
Whereasthe exchange of information provided for in thisDecis ion
should allow for as significant a comparison as possible of the resultsobtained in the sampling and measuring stations;
Whereasthe exchange of information provided for in thisDecis ion
would lay the foundations for a system for monitoring surface fresh-water pollution at Community level and could constitute a componentof the global environmental monitoring system provided for in theUnited Nationsenvironment programme;
Whereas to attain these objectives the Member States must forward to
the Commission data relating to certain parameters for surface fresh
water; whereas the Commission will draw up a consolidated reportwhich it will transmit to the Member States;
Whereas the list of stations in Annex I may, with advantage, be modi-
fied by the Commission at the request of the Member State concerned,provided that certain criteria are fulfilled;
Whereas technical progress requires that the technical specifications
laid down in Annex II to this Decision should be adapted promptly;whereas, to facilitate the implementation of the measures required for
this purpose, provision must be made for a procedure establishing close
cooperation between the Member States and the Commission withinthe Committee for the adaptation of this Decision to technical progress,1977D0795—EN—01.01.199 5—001.001—2
(1) OJ No C 178, 2. 8. 1976, p. 48.
(2) OJ No C 285, 2. 12. 1976, p. 10.
(3) OJ No C 112, 20. 12. 1973, p. 3.
(4) OJ No C 139, 13. 6. 1977, p. 3.
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HAS ADOPTED THIS DECISION:
Article 1
A common procedure for the exchange of information on the quality of
surface fresh water in the Community is hereby established.
Article 2
1. For the purposes of this Decision sampling or measuring stations'
means the stations listed in Annex I.
2. The information concerning the parameters listed in the first
column of Annex II covered by the exchange of information shall be:
(a) the results of the measurements carried out by the sampling or
measuring stations;
(b) a description of the sampling, sample preservation and measuring
methods used and the frequency of sampling.
Article 3
1. Each Member State shall designate a central agency and informthe Commission thereof within 15 days of the notification of this Deci-
sion.
2. The information referred to in Article 2 (2) shall be forwarded to
the Commission through the central agency in each Member State.
3. The data referred to in Article 2 (2) (a) shall be presented
according to the modes of expression and with the significant figures
set out in the second and third columns of Annex II.
The descriptions of the methods referred to in Article 2 (2) (b) may be
omitted if the methods are the same as those used in previous years,
provided always that an explicit reference to any such omission is
made;
4. The information, covering a calendar year, shall be forwarded to
the Commission before 1 October of the following year.
5. The Commission shall forward annually to the Member States
which so request the information received under paragraph 2. Every
three years, and for the first time in 1987, the Commission shall drawup a draft consolidated report based on the information referred to inArticle 2 (2). The part of thisreport concerning the informationsupplied by a Member State shall be sent to the central agency of thatState for verification. Any commentson the draft s hall be included in
the report. The report shall include indications of the trends noted in
water quality since implementation of this Decision and as much inter-pretative comment as possible, taking into account the objectives of theDecision.
The Commission shall publish the final version of its report and send
copiesto the Member States .
6. The Commission shall assess the effectiveness of the procedure
for the exchange of information and shall submit proposals, whereappropriate, to the Council aimed at improving the procedure and, ifnecessary, harmonizing the methods of measurement, taking intoaccount the provisions of Article 4.
Article 4
1. Each Member State shall organize such intercalibration atnational level amongst laboratories taking part in the collection andthe analysis of data as may be necessary to ensure comparability ofreference methods of measurement with those used in the laboratoriesof the Member States.1977D0795—EN—01.01.199 5—001.001—3 
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2. The Commission shall, if necessary, organize a comparative
evaluation of nationally applied methodsof meas urement. Thisevalua-tion shall be the subject of a report to be transmitted to Member States.
3. On the basis of the report referred to in paragraph 2, the Commis-
sion shall, if necessary, make proposals to the Council for theintercalibration of nationally applied methodsof meas urement withthe reference methods of measurement listed in Annex III.
Article 4a
1. In order to implement the common procedure for the exchange ofinformation, the Member Statess hall lay down a frequency forsampling and analysis, normally monthly.
2. If a Member State has established that the water quality does not
display any significant variation in terms of the value of one or more
parametersand if there isno ris k of a deterioration of the water quality,the frequency of sampling and measurement of that parameter or thoseparametersmay be reduced. Such a reduction of the frequency s hall notinvolve any risk to human beings and the environment.
Modificationsto the frequency s hall be explicitly mentioned in the
information forwarded to the Commission under Article 3 (2).
3. The reference methodsof meas urement for the parametersin
question are set out in Annex III. Laboratories which use othermethods of measurement shall satisfy themselves that the results
obtained are comparable.
4. The containers used for samples, the agents or methods used to
preserve part of a sample for the analysis of one or more parameters,the conveyance and storage of samples and the preparation of samplesfor analysis must not be such as to bring about any significant change
in the results of the analysis.
5. Sampling shall take place in the same places and the sampling
procedures shall be the same on each occasion.
Article 5
1. The list in Annex I may be amended by the Commission on arequest from the Member State concerned.
2. The Commission shall make such amendments when it is satisfied
that the following requirementsare met:
— that the list of sampling or measuring stations for each Member
State is sufficiently representative for the purposes of this Decision,
— that the stations are at points which are representative of water
conditionsin the area around and not directly and immediately
influenced by a source of pollution,
— that they are capable of measuring at regular intervals the para-
metersin Annex II,
— that they are asa general rule not more than 100 kilometresapart
on main rivers, not including tributaries,
— that they are upstream of any confluences and not on tidal stretches
of water.
3. The Commission shall inform the Council of any amendments
which it hasaccepted.
4. The Commission shall submit for decision by the Council any
requests for amendments which it has been unable to accept.
Article 6
Amendments necessary to adapt to technical progress the list of para-meters and the modes of expression and significant figures in respectthereof set out in Annex II, as well as the reference methods ofmeasurement, the parameters and the modes of expression set out in1977D0795—EN—01.01.199 5—001.001—4 
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Annex III, shall be adopted in accordance with the procedure laid down
in Article 8, provided that any additionsto the lis t involve only para-meterscovered by Community rulesconcerned with the aquatic
environment and for which data are available in all sampling and
measuring stations of the Member States. Any changes in the modesof expression and significant figures must not involve changes to themethods of measurement used by the Member States in the variousstations in Annex I.
Article 7
1. A Committee for the adaptation of thisDecis ion to technical
progress (hereinafter referred to as the ‘the Committee’) is hereby set
up, consisting of representatives of the Member States with a represen-tative of the Commission as Chairman.
2. The Committee shall adopt its own rules of procedure.
Article 8
1. Where the procedure laid down in thisArticle isto be followed,
the matter shall be referred to the Committee by its chairman, either onhis own initiative or at the request of a representative of a MemberState.
2. The Commission representative shall submit to the Committee a
draft of the measures to be taken. The Committee shall give its opinion
on the draft within a time limit set by the chairman according to theurgency of the matter. Opinionss hall be delivered by a majority of►
A362◄votes, the votes of the Member States being weighted as
provided for in Article 148 (2) of the Treaty. The chairman shall notvote.
3. (a) Where the measures envisaged are in accordance with the
opinion of the Committee, the Commission shall adopt them.
(b) Where the measures envisaged are not in accordance with the
opinion of the Committee, or if no opinion isdelivered, the
Commission shall without delay submit to the Council a
proposal on the measures to be taken. The Council shall act by
a qualified majority.
(c) If within three months of the proposal being submitted to it the
Council has not acted, the proposed measures shall be adopted
by the Commission.
Article 9
This Decision is addressed to the Member States.1977D0795—EN—01.01.199 5—001.001—5 
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ANNEX I
LIST OF SAMPLING OR MEASURING STATIONS INVOLVEDIN THE EXCHANGE OF
INFORMATION
FEDERAL REPUBLIC OF GERMANY
Sampling or measuring stations List of rivers
Maxau 362·3 km downstream of the Rhine bridge at Constance Rhine
Mainz 498·5 km downstream of the Rhine bridge at Constance Rhine
Koblenz/Brau-
bach590·3 km downstream of the Rhine bridge at Constance Rhine
Palzem 230·3 km upstream of the confluence with the Rhine Moselle
Koblenz/
Moselle2 km upstream of the confluence with the Rhine Moselle
Kleve/Bimmen 864·95 km downstream of the Rhine bridge at Constance at the
point where the river leavesthe Federal Republic of GermanyRhine
Duisburg 1,0 km upstream of the confluence with the Rhine Ruhr
Herbrüm 284·9 km from the source (212·04 Canal km) Ems
Hemeln 11 km downstream of the confluence of the Werra and the
FuldaWeser
Intschede 329·7 km downstream of the confluence of the Werra and the
FuldaWeser
Geesthacht 113 km downstream of the entry of the river into the Federal
Republic of Germany (584·5 Elbe km)Elbe
Jochenstein 2 203 km upstream of the mouth of the Danube Danube
BELGIUM
Sampling or measuring stations List of rivers
Warneton downstream of the confluence with the Deule LysLeers-Nord at the point where the river enters Belgium Espierre
Doel at the point where the river leavesBelgium ScheldtBléhariesat the point where the river enters Belgium Scheldt
Erquelinnesat the point where the river enters Belgium Sambre
Heer-Agimont at the point where the river entersBelgium Meus e
Lanaye-
Ternaaienat the point where the river leavesBelgium Meus e
Martelange at the point where the river leavesBelgium Sure
Zelzate at the point where the river leavesBelgium Ghent-
Terneuzen
Canal
DENMARK
Sampling or measuring stations List of rivers
Nåby Station No 57.12 Suså
Nr Broby Station No 45.01 Odense Å
Tvilum bro Station No 21.01 Gudenå
Ahlergård Station No 25.05 Skjern Å1977D0795—EN—01.01.199 5—001.001—6 
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GREECE
Sampling or measuring station List of rivers
Drama PapadesBridge (01) (30 km from the point where the river
entersGreece)Nestos
SerresSidirokas tro Bridge (02) (18 km from the point where the river
entersGreece)Strymon
Thessaloniki Kilometre post 61 · 500 on the Thessaloniki-Idomeni railway
line (03) (10 km from the point where the river entersGreece)Axios
Kozani Moni Ilarion (04) upstream of the Polyphyte reservoir (150 km
upstream of the mouth)Aliakmon
Larissa Larissa municipality water-tower (05) (50 km upstream of the
mouth)Pinios
Agrinion Kastraki (06) downstream of the reservoir (50 km upstream of
the mouth)Akheloos
FRANCE
Sampling or measuring stations List of rivers
Méry upstream of the confluence with the Aube (St 6 000) Seine
Montereau upstream of the confluence with the Yonne (St 14 000) Seine
Ponthierry Bridge RD 50 (St 48 000) SeineParisTolbiac Bridge (St 81 000) Seine
St Rambert Andrézieux Bridge downstream of St Rambert (St 9 000) Loire
Brienon downstream of the Pouilly-sous-Charlieu Bridge RN 487 R.G.
(St 15 000)Loire
Orléansups tream of Orléans (St 50 500) Loire
La Possonière SNCF bridge downstream of La Possonière (St 134 500) Loire
Toulouse downstream of Toulouse (St 161 000) Garonne
Lamagistère downstream of the confluence with the Aveyron (St 117 000) Garonne
Couthures near Couthures, downstream of the confluence with the Avance
(St 81 000)Garonne
Auxonne France Bridge (St 11 000) Saône
Lyon upstream of the confluence with the Rhône at the Mazaryk
Bridge (St 59 500)Rhône
Pont de
Pougnyat the point where the Rhône entersFrance (St 65 700) Rhône
Lyon upstream of the confluence with the Saône at the Poincaré
Bridge (St 93 000)Rhône
St Vallier upstream of the confluence with the Isère (St 104 000) Rhône
IRELAND
Sampling or measuring stations List of rivers
Slane Bridge approximately 12 km downstream of Navan (N 96 74) Boyne
Corofin Bridge approximately 19 km downstream of Tuam (M 42 43) Clare
Graiguenama-
nagh Bridgeapproximately 29 km downstream of Muine Bheag (Bagenals-town) (S 71 44)Barrow
KillavullenBridgeapproximately 13 km downstream of Mallow (W 65 99) Blackwater
(Munster)1977D0795—EN—01.01.199 5—001.001—7 
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ITALY
Sampling or measuring stations List of rivers
Bronzolo-
Ponte Vadena300 km upstream of the mouth Adige
Trento 253 km upstream of the mouth Adige
Badia Polesine 66 km upstream of the mouth Adige
Moncalieri 594 km upstream of the mouth PoCremona 281 km upstream of the mouth Po
Boretto 216 km upstream of the mouth Po
Borgo Forte 184 km upstream of the mouth Po
Pontelagoscuro 91 km upstream of the mouth Po
Ponte degli
Alberi38 km upstream of the mouth Metauro
Buon Riposo 186 km upstream of the mouth Arno
Nave di
Rosano120 km upstream of the mouth Arno
Capraia 70 km upstream of the mouth Arno
Calcinaia 38 km upstream of the mouth ArnoPonte Felcino 300 km upstream of the mouth Tiber
Ponte Nuovo 273 km upstream of the mouth Tiber
Ponte Ripetta 43 km upstream of the mouth Tiber
GRAND DUCHY OF LUXEMBOURG
Sampling or measuring stations List of rivers
Wasserbillig upstream of the confluence with the Moselle Sûre
NETHERLANDS
Sampling or measuring stations List of rivers
Lobith at the point where the river entersthe Netherlands Upper RhineKampen 123 km downstream of the point where the Rhine enters the
NetherlandsIjssel
Vuren 88 km downstream of the point where the Rhine enters the
NetherlandsUpper
Merwede
Hagestein 82 km downstream of the point where the Rhine enters the
NetherlandsLek
OM 42 Puttershoek, 120 km downstream of the point where the Rhine
entersthe NetherlandsOude Maas
NM 34 near the island of Brienenoord, 134 km downstream of the point
where the Rhine entersthe NetherlandsNieuwe Maas
Eijsden 4·5 km downstream of the point where the Meuse enters the
NetherlandsMeuse1977D0795—EN—01.01.199 5—001.001—8 
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Sampling or measuring stations List of rivers
Lith 201 km downstream of the point where the Meuse enters the
NetherlandsMeuse
Keizersveer 248 km downstream of the point where the Meuse enters the
NetherlandsBergse Maas
H 9 Haringvlietbrug (Haringvliet Bridge) Haringvliet
H 12 near the Haringvlietdam Haringvliet
IJ 12 Ketelbrug, 145 km downstream of the point where the Rhine
entersthe NetherlandsKetelmeer
IJ 23 centre of Lake Ijssel Lake Ijssel
UNITED KINGDOM
Sampling or measuring stations List of rivers
Chollerford 6 km upstream of the confluence (NY 919 706) North Tyne
Warden Bridge 800 m upstream of the confluence (NY 910 660) South Tyne
Wylam Bridge immediately upstream of the tidal limit (NZ 119 645) Tyne
Derwenthaugh 1·3 km upstream of the tidal limit (NZ 187 607) Derwent
Whitford
Bridge3 km upstream of the tidal limit (SY 262 953) Axe
TregonyGauging
Station6 km upstream of the tidal limit (SW 921 445) Fal
DevoranBridgeimmediately upstream of the tidal limit (SW 791 394) Carnon
Denny Bridge 0,5 km upstream of the tidal limit (SD 504 647) Lune
St Michael's
Weirimmediately upstream of the tidal limit (SD 462 411) Wyre
Samlesbury 1·5 km upstream of the tidal limit (SD 589 304) Ribble
Teddington
Weir(TQ 171 714) Thames
Chetwynd (SK 187 138) Tame
Nottingham (SK 581 383) Trent
Yoxall (SK 131 177) Trent
Fochabers4 km ups tream of the tidal limit (NJ 341 596) Spey
Craigiehall 3 km upstream of the tidal limit (NT 165 752) Almond
Renton Foot-
bridgeimmediately upstream of the tidal limit (NS 389 783) Leven
SPAIN
Sampling or measuring stations List of rivers
San Esteban de
Gormazstation no02.07 Douro
Villamarciel station no02.54 Douro
Puente Pino station no02.53 Douro
Trillo station no03.93 Tagus
Aranjuez station no03.11 Tagus
Talavera de la
Reinastation no03.15 Tagus1977D0795—EN—01.01.199 5—001.001—9 
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Sampling or measuring stations List of rivers
Alcántara station no03.19 Tagus
Balbuena station no04.08 Guadiana
Badajoz station no04.18 Guadiana
Menjibar station no05.04 Guadalquivir
Peñaflor station no05.06 Guadalquivir
Sevilla station no05.74 Guadalquivir
Miranda de
Ebrostation no09.01 Ebro
Zaragoza station no09.11 Ebro
Tortosa station no09.27 Ebro
PORTUGAL
Sampling or measuring stations List of rivers
LanhelasStation no01.1 Minho
Messegães Station no01.4 Minho
Barragem deCrestumaStation No 09.2 Douro
Pinhão Station No 09.6 Douro
S. João deLoureStation n
o12.2 Vouga
Penacova Station no16.4 Mondego
Santarém Station no30.3 Tagus
PeraisStation no30.10 Tagus
Castelo de
BodeStation no30.20.2 Zêzere
Mértola Station no54.3 Guadiana'
Sada Ajuda Station no57.7 Guadiana'
AUSTRIA
Sampling or measuring stations List of rivers
Jochenstein 2 203,8 km upstream of the mouth Danube
Abwinden-
Asten2 119,9 km upstream of the mouth Danube
Wolfsthal 1 873,5 km upstream of the mouth Danube
Lavamünd 2,1 km upstream the point where the Drau leaves Austria Drau
Kufstein/Erl 204,03 km upstream of the confluence with the Danube Inn
Oberndorf 47,2 km upstream the confluence with the Inn Salzach
Bad Radkers-
burg101,4 km upstream the confluence with the Drau Mur
FINLAND
Sampling or measuring stations List of rivers
Kalkkisten-
koskiStation No 4800, lake outlet of lake Päijänne Kymi1977D0795—EN—01.01.199 5—001.001—10 
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Sampling or measuring stations List of rivers
Pori-Tampere
BridgeStation No 8820, 7,5 km upstream of Pori Kokemäenjoki
Mansikkakoski Station No 2800, lake outlet of lake Saimaa Vuoksi
Raasakka
Bridge8,0 km upstream of Ii Ii
MerikoskiBridgeStation No 13000, Oulu City Oulujoki
IsohaaraBridgeStation No 14000, Kemi City Kemijoki
Kukkolankoski Station No 14310, 13 km upstream of Tornio Torniojoki
Virtaniemi Station No 14400, lake outlet of Lake Inari Paatsjoki
SWEDEN
Sampling or measuring stations List of rivers
Luleå Station No 009 Lule älv
StornorrforsStation No 028 Ume älvBergeforsen Station No 040 Indalsälven
Älvkarleby Station No 053 Dalälven
Stockholm Station No 061 Norrström
Norrköping Station No 067 Motala ström
Mörrum Station No 086 Mörrumsån
Helsingborg Station No 094 Råån
Laholm Station No 098 Lagan
Aleyckan Station No 108 Göta älv1977D0795—EN—01.01 .199 5—001.001—11
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ANNEX II
PARAMETERSINRESPECTOFWHICHINFORMATIONISTOBEEXCHANGED
(Modesofexpressionandsignificantfiguresfortheparametric data)
ParameterMode of expres-
sionSignificant figures
Before the decimal
commaAfter the decimal
comma
physicalRate of flow (1) (at the
time of sampling)m3/sec xxxx xx
Temperature ºC xx x
pH pH xx x
Conductivity at 20 ºC µS cm−1(<100) xx
(≥100) xxx
chemicalChloridesCl mg/l(<100) xx
(≥100) xxx
NitratesNO3mg/l xxx xx
Ammonia NH4mg/l xxx xx
Dissolved oxygen O2mg/l xx x
BOD5O2mg/l xxx x
COD O2mg/l xxx x
Total phosphorus P mg/l xx xx
Surfactantsreacting to
methylene blueSodium laurylsulphate eq.
mg/lxx xx
Total cadmium Cd mg/l x xxxx
Mercury Hg mg/l x xxxx
microbiologicalFaecal coliforms/ 100 ml xxxxxx
Total coliforms(
2) / 100 ml xxxxxx
Faecal streptococci (2) / 100 ml xxxxxx
Salmonella (2)/ 1 l x
biological biological quality (2)(3)
(1) The date of sampling must be given.
(2) The data relating to this parameter shall be exchanged when it is measured.
(3) The frequency of sampling of this parameter and the mode of expression of results shall be decided on by
Member States.1977D0795—EN—01.01.199 5—001.001—12
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ANNEX III
REFERENCEMETHODSOFMEASUREMENT
ParameterMode of
expressionReference method of measurement
Rate of flow at the time
of samplingm3/sFlowmeter
Temperature ºC Thermometry
measured in situ at the time of sampling
pH pH Electrometrymeasured in situ at the time of sampling without prior
treatment of the sample
Conductivity at 20 ºC µS cm
−1Electrometry
ChloridesCl mg/l Titration (Mohr'smethod)Molecular absorption spectrophotometry
NitratesNO
3mg/l Molecular absorption spectrophotometry
Ammonia NH4mg/l Molecular absorption spectrophotometry
Dissolved oxygen O2mg/l Winkler'smethod
Electrochemical method
BOD5O2mg/l Determination of dissolved oxygen before and after five-
day incubation at 20 ± 1 ºC in complete darkness. Addi-
tion of a nitrification inhibitor.
COD O2mg/l Potassium dichromate method
Total phosphorus P mg/l Molecular absorption spectrophotometry
Surfactantsreacting to
methylene blueSodium
lauryl
sulphate
eq. mg/lMolecular absorption spectrophotometry
Total cadmium Cd mg/l Atomic absorption spectrophotometry
Mercury Hg mg/l Flameless atomic absorption spectrophotometry
Faecal coliforms / 100 ml — Culture at 44 ºC on an appropriate specific solid
medium (such as Tergitol lactose agar, Endo agar,
0,4 % Teepol broth) with or without filtration and
colony count. Samplesmus t be diluted or, where
appropriate, concentrated in such a way as to contain
between 10 and 100 colonies. If necessary, identifica-
tion by gasification.
— Method of dilution with fermentation in liquid
substrates in at least three tubes in three dilutions.Sub-culturing of the positive tubes on a confirmation
medium. Count according to CONS.ACTN (most
probable number). Incubation temperature: 44 ± 0,5 ºC.1977D0795—EN—01.01.199 5—001.001—13
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ParameterMode of
expressionReference method of measurement
Total coliforms/100 ml — Culture at 37 ºC on an appropriate specific solid
medium (such as Tergitol lactose agar, Endo agar,
0,4 % Teepol broth) with or without filtration and
colony count. Samplesmus t be diluted or, where
appropriate, concentrated in such a way as to contain
between 10 and 100 colonies. If necessary, identifica-
tion by gasification.
— Method of dilution with fermentation in liquid
substrates in at least three tubes in three dilutions.
Subculturing of the positive tubes on a confirmation
medium. Count according to CONS.ACTN (most
probable number). Incubation temperatures: 37 ºC
± 1 ºC.
Faecal streptococci /100 ml — Culture at 37 ºC on an appropriate solid medium (such
ass odium azide) with or without filtration and colonycount.
— Method of dilution in sodium azide broth (Litsky).
Count according to CONS.ACTN (most probable
number).
Salmonella /1 l Concentration by filtration (on membrane or appropriate
filter). Innoculation (SIC! Inoculation) into pre-enriche-
ment (SIC! pre-enrichment) medium. Enrichment and
transfer into isolating gelose — identification.
Biological quality Pending Community-wide harmonization, Member Stateswill us e their
respective methods.1977D0795—EN—01.01.199 5—001.001—14