Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation

Content of element (Mn) in mg/kg of complete feed with a moisture content of 12 %

Category of nutritional additives. Functional group: compounds of trace elements

3b501 — Manganous chloride tetrahydrate Additive composition Manganous chloride tetrahydrate, as a powder, with a minimum content of 27 % manganese. Characterisation of the active substance Manganous chloride tetrahydrate Chemical formula: MnCl2 · 4H2O CAS Number: 13446-34-9 Analytical methods (1) For the identification reactions of chloride in the feed additive: — European Pharmacopoeia Monograph 2.3.1; For the crystallographic characterisation of the feed additive: — X-Ray diffraction; For the quantification of total manganese in the feed additive and premixtures: — Atomic Absorption Spectrometry,AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621); For the quantification of total manganese in feed materials and compound feed: — Atomic Absorption Spectrometry, AAS (Commission Regulation (EC) No 152/2009 (2), Annex IV-C); or — Atomic Absorption Spectrometry, AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621). All animal species — — Fish: 100 (total) Other species: 150 (total) 1. The additive shall be incorporated into feed in the form of a premixture. 2. Manganous chloride tetrahydrate may be placed on the market and used as an additive consisting of a preparation. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and appropriate organisational measures to address the potential risks by inhalation, dermal contact or eyes contact, in particular due to the content of heavy metals including nickel. Where risks cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment. 11 September 2027

3b502 — Manganese (II) oxide Additive composition Manganous oxide, as a powder, with a minimum content of 60 % manganese; Minimum content of 77,5 % MnO and a maximum content of 2 % MnO2 Characterisation of the active substance Manganous oxide Chemical formula: MnO CAS Number: 1344-43-0 Analytical methods (1) For the crystallographic characterisation of the feed additive: — X-Ray diffraction; For the quantification of total manganese in the feed additive and premixtures: — Atomic Absorption Spectrometry,AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621); For the quantification of total manganese in feed materials and compound feed: — Atomic Absorption Spectrometry, AAS (Regulation (EC) No 152/2009, Annex IV-C); or — Atomic Absorption Spectrometry, AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621). All animal species — — Fish: 100 (total) Other species: 150 (total) 1. The additive shall be incorporated into feed in the form of a premixture. 2. Manganese (II) oxide may be placed on the market and used as an additive consisting of a preparation. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and appropriate organisational measures to address the potential risks by inhalation, dermal contact or eyes contact, in particular due to the content of heavy metals including nickel. Where risks cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment. 11 September 2027

3b503 — Manganous sulphate monohydrate Additive composition Manganous sulphate monohydrate, as a powder, with a minimum content of 95 % manganous sulphate monohydrate and of 31 % manganese. Characterisation of the active substance Manganous sulphate monohydrate Chemical formula: MnSO4 · H2O CAS Number: 10034-96-5 Analytical methods (1) For the quantification of the manganous sulphate monohydrate in the feed additive: — titration with ammonium and cerium nitrate (Ph. Eur Monograph 1543); For the identification reactions of sulphates in the feed additive: — European Pharmacopoeia Monograph 2.3.1; For the crystallographic characterisation of the feed additive: — X-Ray diffraction; For the quantification of total manganese in the feed additive and premixtures: — Atomic Absorption Spectrometry,AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621); For the quantification of total manganese in feed materials and compound feed: — Atomic Absorption Spectrometry, AAS (Regulation (EC) No 152/2009, Annex IV-C); or — Atomic Absorption Spectrometry, AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621). All animal species — — Fish: 100 (total) Other species: 150 (total) 1. The additive shall be incorporated into feed in the form of a premixture. 2. Manganous sulphate monohydrate may be placed on the market and used as an additive consisting of a preparation. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and appropriate organisational measures to address the potential risks by inhalation, dermal contact or eyes contact, in particular due to the content of heavy metals including nickel. Where risks cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment. 11 September 2027

3b504 — Manganese chelate of amino acids hydrate Additive composition Preparation of manganese amino acid complex where the manganese and the amino acids derived from soya protein are chelated via coordinate covalent bonds, as a powder with a minimum content of 8 % manganese. All animal species — — Fish: 100 (total (3)) Other species: 150 (total (3)) 1. The additive shall be incorporated into feed in the form of a premixture. 2. For users of the additive and premixtures, feed business operators shall establish operational procedures and appropriate organisational measures to address the potential risks by inhalation, dermal contact or eyes contact, in particular due to the content of heavy metals, including nickel. Where risks cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment, including skin, eye and breathing protection. 11 September 2027

Characterisation of the active substance Chemical formula: Mn(x)1–3 nH2O, x = anion of any amino acid derived from acid hydrolysed soya protein; Maximum 10 % of molecules exceeding 1 500 Da.

Analytical method (1) For the quantification of amino acid content in the feed additive: — ion exchange chromatography with post-column derivatisation and optical detection (IEC-VIS/FLD) For the quantification of total manganese in the feed additive: — inductively coupled plasma-atomic emission spectrometry, ICP-AES (EN 15510 or EN 15621) or — atomic absorption spectrometry, AAS (ISO 6869) For the quantification of total manganese in premixtures: — inductively coupled plasma-atomic emission spectrometry, ICP-AES (EN 15510 or EN 15621) or — atomic absorption spectrometry, AAS (ISO 6869) or — inductively coupled plasma-mass spectrometry, ICP-MS (EN 17053) For the quantification of total manganese in feed materials and compound feed: — inductively coupled plasma-atomic emission spectrometry, ICP-AES (EN 15510 or EN 15621) or — atomic absorption spectrometry, AAS (Commission Regulation (EC) No 152/2009 (Annex IV-C) or ISO 6869) or — inductively coupled plasma-mass spectrometry, ICP-MS (EN 17053)

3b504i — Manganese chelate of amino acids hydrate Additive composition Preparation of manganese amino acid complex, where the manganese and the amino acids are chelated via coordinate covalent bonds, as a powder with a content of 8 – 9 % manganese and a minimum of 17 % free amino acids. All animal species — — Fish: 100 (total (3)) Other species: 150 (total (3)) 1. The additive shall be incorporated into feed in the form of a premixture. 2. For users of the additive and premixtures, feed business operators shall establish operational procedures and appropriate organisational measures to address the potential risks by inhalation, dermal contact or eyes contact, in particular due to the content of heavy metals, including nickel. Where risks cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment, including skin, eye and breathing protection. 3. For additives produced from hydrolysis of animal protein, the animal origin (avian species) shall be indicated on the label of the additive and premixtures 11 September 2027

Characterisation of the active substance: Chemical formula: Mn(x)1-3•nH2O, where x is equal to any amino acid coming from hydrolysed protein sources from feathers or plants; Maximum 10 % of molecules exceeding 1 500 Da.

Analytical method (1) For the quantification of amino acid content in the feed additive: — ion exchange chromatography with post-column derivatisation and optical detection (IEC-VIS/FLD), Commission Regulation 152/2009 (Annex III,F) and EN ISO 17180 For the quantification of total manganese in the feed additive: — inductively coupled plasma-atomic emission spectrometry, ICP-AES (EN 15510 or EN 15621) or — atomic absorption spectrometry, AAS (ISO 6869) For the quantification of total manganese in premixtures: — inductively coupled plasma-atomic emission spectrometry, ICP-AES (EN 15510 or EN 15621) or — atomic absorption spectrometry, AAS (ISO 6869) or — inductively coupled plasma-mass spectrometry, ICP-MS (EN 17053) For the quantification of total manganese in feed materials and compound feed: — inductively coupled plasma-atomic emission spectrometry, ICP-AES (EN 15510 or EN 15621) or — atomic absorption spectrometry, AAS (Commission Regulation (EC) No 152/2009 (Annex IV-C) or ISO 6869) or — inductively coupled plasma-mass spectrometry, ICP-MS (EN 17053)

3b505 — Manganese chelate of protein hydrolysates Additive composition Manganese chelate of protein hydrolysates as a powder with a minimum content of 10 % manganese. Minimum of 50 % manganese chelated. Characterisation of the active substance Chemical formula: Mn(x)1–3 · nH2O, x = anion of protein hydrolysates containing any amino acid from soya protein hydrolysate. Analytical methods (1) For the quantification of protein hydrolysates content in the feed additive: — ion exchange chromatography combined with post-column ninhydrin derivatisation and photometric detection (Regulation (EC) No 152/2009, Annex III, F); For the determination of chelated manganese content in the feed additive: — Fourier Transformed Infrared (FTIR) spectroscopy followed by multivariate regression methods. For the quantification of total manganese in the feed additive and premixtures: — Atomic Absorption Spectrometry,AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621); For the quantification of total manganese in feed materials and compound feed: — Atomic Absorption Spectrometry, AAS (Regulation (EC) No 152/2009, Annex IV-C); or — Atomic Absorption Spectrometry, AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621). All animal species — — Fish: 100 (total) Other species: 150 (total) 1. The additive shall be incorporated into feed in the form of a premixture. 2. Manganese chelate of protein hydrolysates may be placed on the market and used as an additive consisting of a preparation. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and appropriate organisational measures to address the potential risks by inhalation, dermal contact or eyes contact, in particular due to the content of heavy metals including nickel. Where risks cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment. 11 September 2027

3b506 — Manganese chelate of glycine hydrate Additive composition Manganese chelate of glycine, hydrate, as a powder with a minimum content of 15 % manganese. Moisture: maximum 10 %. Characterisation of the active substance Chemical formula: Mn(x)1-3 · nH2O, x = anion of glycine. Analytical methods (1) For the quantification of the glycine content in the feed additive: — ion exchange chromatography combined with post-column ninhydrin derivatisation and photometric detection (Regulation (EC) No 152/2009, Annex III, F); For the quantification of total manganese in the feed additive and premixtures: — Atomic Absorption Spectrometry,AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621); For the quantification of total manganese in feed materials and compound feed: — Atomic Absorption Spectrometry, AAS (Regulation (EC) No 152/2009, Annex IV-C); or — Atomic Absorption Spectrometry, AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621). All animal species — — Fish: 100 (total) Other species: 150 (total) 1. The additive shall be incorporated into feed in the form of a premixture. 2. Manganese chelate of glycine hydrate may be placed on the market and used as an additive consisting of a preparation. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and appropriate organisational measures to address the potential risks by inhalation, dermal contact or eyes contact, in particular due to the content of heavy metals including nickel. Where risks cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment. 11 September 2027

3b507 — Dimanganese chloride trihydroxide Additive composition Granulate powder with a minimum content of 44 % manganese and a maximum content of 7 % manganese oxide Characterisation of the active substance Dimanganese chloride trihydroxide Chemical formula: Mn2(OH)3Cl CAS Number: 39438-40-9 Analytical methods (1) For the identification of the crystallographic characterisation of the feed additive: — X-Ray diffraction; For the quantification of chlorine in the feed additive: — Titration — Regulation (EC) No 152/2009; For the quantification of total manganese in the feed additive and premixtures: — Atomic Absorption Spectrometry, AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621); For the quantification of total manganese in feed materials and compound feed: — Atomic Absorption Spectrometry, AAS (Regulation (EC) No 152/2009, Annex IV-C); or — Atomic Absorption Spectrometry, AAS (EN ISO 6869); or — Inductively Coupled Plasma — Atomic Emission Spectrometry, ICP-AES (EN 15510); or — Inductively Coupled Plasma — Atomic Emission Spectrometry after pressure digestion, ICP-AES (CEN/TS 15621). All animal species — — Fish: 100 (total) Other species: 150 (total) 1. The additive shall be incorporated into feed in the form of a premixture. 2. Dimanganese chloride trihydroxide may be placed on the market and used as an additive consisting of a preparation. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and appropriate organisational measures to address the potential risks by inhalation, dermal contact or eyes contact, in particular due to the content of heavy metals including nickel. Where risks cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment. 11 September 2027