Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation

mg/kg of complete feedingstuff with a moisture content of 12 %

Category of nutritional additives. Functional group: amino acids, their salts and analogues

3c301 DL-methionine, technically pure Additive composition Methionine content: minimum 99 % Characterisation of the active substance IUPAC name: 2-amino-4-(methylthio)butanoic acid CAS number: 59-51-8 Chemical formula: C5H11NO2S Analytical methods (1) For the determination of methionine in the additives: — Ion exchange chromatography coupled with post-column derivatisation and photometric or fluorescence detection (HPLC-UV/FD) - ISO/DIS 17180. For the determination of methionine in premixtures, compound feed, feed materials and water: — Ion exchange chromatography coupled with post-column derivatisation and photometric detection (HPLC-UV) - Commission Regulation (EC) No 152/2009 (Annex III, F). all animal species 1. DL-methionine, technically pure can be also used via water for drinking. 2. Declarations to be made on the labelling of the additive and premixtures: ‘If the additive is administered via water for drinking protein excess should be avoided.’ 12 June 2023

3c302 Sodium DL-methionine, liquid Additive composition DL-Methionine content: minimum 40 % Sodium: minimum 6,2 % Water: maximum 53,8 % Characterisation of the active substance IUPAC name: 2-amino-4-(methylthio)butanoic acid sodium salt CAS number: 41863-30-3 Chemical formula: (C5H11NO2S)Na Analytical methods For the determination of methionine in the additives: — Ion exchange chromatography coupled with post-column derivatisation and photometric or fluorescence detection (HPLC-UV/FD) - ISO/DIS 17180. For the determination of methionine in premixtures, compound feed, feed materials and water: — Ion exchange chromatography coupled with post-column derivatisation and photometric detection (HPLC-UV) - Commission Regulation (EC) No 152/2009 (Annex III, F). all animal species 1. For user safety: breathing protection, safety glasses and gloves should be worn during handling. 2. Sodium DL-methionine, liquid can be also used via water for drinking. 3. Declarations to be made on the labelling of the additive and premixtures: — DL-Methionine content, — ‘If the additive is administered via water for drinking protein excess should be avoided.’ 12 June 2023

3c303 DL-methionine protected with copolymer vinylpyridine/styrene Additive composition Preparation with DL-Methionine: minimum 74 % Stearic acid: maximum of 19 % Copolymer poly(2-vinylpyridine) co-styrene: maximum 3 % Ethylcellulose and sodium stearate: maximum 0,5 % Characterisation of the active substance IUPAC name: 2-amino-4-(methylthio)butanoic acid CAS number: 59-51-8 Chemical formula: C5H11NO2S Analytical methods (1) For the determination of methionine in the additives: — Ion exchange chromatography coupled with post-column derivatisation and photometric or fluorescence detection (HPLC-UV/FD) - ISO/DIS 17180. For the determination of methionine in premixtures, compound feed and feed materials: — Ion exchange chromatography coupled with post-column derivatisation and photometric detection (HPLC-UV) - Commission Regulation (EC) No 152/2009 (Annex III, F). Ruminants 12 June 2023

3c304 DL-methionine protected with ethylcellulose Additive composition Preparation with DL-Methionine: minimum 85 % Ethylcellulose: maximum 4 % Starch: maximum 8 % Sodium aluminium silicate: maximum 1,5 % Sodium stearate: maximum 1 % Water: maximum 2 % Characterisation of the active substance IUPAC name: 2-amino-4-(methylthio)butanoic acid CAS number: 59-51-8 Chemical formula: C5H11NO2S Analytical methods (1) For the determination of methionine in the additives: — Ion exchange chromatography coupled with post-column derivatisation and photometric or fluorescence detection (HPLC-UV/FD) - ISO/DIS 17180. For the determination of methionine in premixtures, compound feed and feed materials: — Ion exchange chromatography coupled with post-column derivatisation and photometric detection (HPLC-UV) - Commission Regulation (EC) No 152/2009 (Annex III, F). Ruminants 12 June 2023

3c307 — Hydroxy analogue of methionine Additive composition Hydroxy analogue of methionine: minimum 88 % Water: maximum 12 % Characterisation of the active substance IUPAC name: 2-hydroxy-4-(methylthio)butanoic acid CAS number 583-91-5 Chemical formula: C5H10O3S Analytical methods (1) For the determination of hydroxy analogue of methionine in the additive: — Titrimetry, potentiometric titration followed by oxidation reduction reaction. For the determination of hydroxy analogue of methionine in premixtures, compound feed, feed materials and water: — High-Performance Liquid Chromatography and photometric detection (HPLC-UV). All animal species — — — 1. For user safety: breathing protection, safety glasses and gloves should be worn during handling. 2. Hydroxy analogue of methionine can be also used via water for drinking. 3. Declarations to be made on the labelling of the additive and premixtures: — ‘If the additive is administered via water for drinking protein excess should be avoided.’ 4. Declarations to be made on the labelling of feed materials and compound feed in the listing of additives, if appropriate: — Name of the additive, — Amount of the hydroxy analogue of methionine added. 12 June 2023

►C1 3c308 ◄ — Calcium salt of hydroxy analogue of methionine Additive composition Hydroxy analogue of methionine: minimum 84 % Calcium: minimum 11,7 % Water: maximum 1 % Characterisation of the active substance IUPAC name 2-hydroxy-4-(methylthio)butanoic acid, calcium salt CAS number 4857-44-7 Chemical formula: (C5H9O3S)2Ca Analytical methods (1) For the determination of hydroxy analogue of methionine in the additive: — Titrimetry, potentiometric titration followed by oxidation reduction reaction. For the determination of hydroxy analogue of methionine in premixtures, compound feed and feed materials: — High-Performance Liquid Chromatography and photometric detection (HPLC-UV). All animal species — — — 1. For user safety: breathing protection, safety glasses and gloves should be worn during handling. 2. Declarations to be made on the labelling of the additive and premixtures: — Hydroxy analogue of methionine content. 3. Declarations to be made on the labelling of feed materials and compound feed in the listing of additives, if appropriate: — Name of the additive, — Amount of the hydroxy analogue of methionine added. 12 June 2023

3c309 — Isopropyl ester of hydroxy analogue of methionine Additive composition Preparation of isopropyl ester of hydroxy analogue of methionine: minimum 95 % Water: maximum 0,5 % Characterisation of the active substance IUPAC name isopropyl ester of 2-hydroxy-4-(methylthio)butanoic acid CAS number 57296-04-5 Chemical formula: C8H16O3S Analytical method For the determination of isopropyl ester of the hydroxy analogue of methionine in the feed: — High-Performance Liquid Chromatography and photometric detection (HPLC-UV). Ruminants — — — 1. Declarations to be made on the labelling of the additive and premixtures: — Content of hydroxy analogue of methionine 2. Declarations to be made on the labelling of feed materials and compound feed in the listing of additives, if appropriate: — Name of the additive, — Amount of the hydroxy analogue of methionine added. 12 June 2023