Her research is focused mainly on marine symbiosis, behavioral ecology, and conservation biology in temperate New England, the Phoenix Islands Protected Area [Kiribati], and in Belize. Rotjan has a joint position with the New England Aquarium Anderson Cabot Center for Ocean Life, where she previously maintained a research lab for eight years. She has been in the Boston area for awhile, completing her postdoctoral work at Harvard and her doctoral work at Tufts. She is a member of the Explorers Club and Women Working for Oceans, and is deeply passionate about oceans, conservation action, scientific literacy, and education.