The San Francisco call., 1896-05-31

General the effect of the tuloidIn stain is as
follows: The plasmophere stains family blue,
with darker blue blotches along the outer
margin. The hyalosphere or annulus almost
pure white. The centrosphere more or less
deep violet The microsphere either pale
whitish or deeper violet or blue than the CEN
trosphere. The somosphere dark with the
centrosomes darkest. From this general rule
there are, however, few exceptions.
If we study this figure closer we see that the
cytoplasmic projection is especially prominent
at the upper pole, while at the lower pole it is
quite small. We have no difficulty in recog-
nizing the outer plasmosphere, with its fringe
! like Margin the hyalosphere next to it, almost
unstained, and the granosphere, which is very
large at the upper pole, while at the lower pole
it is very narrow. The mierosphere again
is in this corpusoie stained dark, while the
somosphere and centrosomes are stained the
darkest of the spheres.
In the other class of fusiform corpuscles
again we find that the microsphere, at either
end, has travelled away from the base OF the
cone, toward the apex. much more so atthe
pole. We find that ct the pole the spheres
are arranged in the cone-like type, while at
| pole a the spheres have assumed the star type,
| that is, they have travelled away from the base,
| there being about as much centrosphere on
each side surrounding the microsphere. AS
/
stages between the two extremes, and the in
! dividuality of each is such that two corpus
1 cles are never found exactly alike. Still there
/ is such great general similarity that we are
rarely in doubt regards the identity of the
| respec tive zones or spheres. will now refer
| to each OF the three spheres in detail, begin
ming with the outer one on the plasmosphere.
PLAsMosPHERE.-The plasmosphere is espe-
cially developed at the poles. 1t may be much
Z ~ r,o1o thar nd ,, fc
inequality IN size corresponds also with the
! inequality in size of the other spheres. In
other words, the spheres increase in size to-
| gether, ana in such way that the largest
| spheres are always those which surround the
centromes and centrosphere. The plasmo-
sphere appears always fringed, partly from
actually being so. partly from dark staining
cytoplasm arranged as a dotted border. This
fringed appearance resembles greatly the
plasma projections of an am9ba and in a
general way the direction OF the rays are from
the center outward, like the rays of a star.
The plasmosphere gives the impression as if ii
sometimes were in rest, while at other times
| it appears as if it had been fixed while in
ameboid activity.
| HYALosPHERE OR ANNULUs-Treateo with
tuloidin this sphere appears always trans
parent and well defined as narrow, even
generally highly refractive annulus, which
surrounds both the oentrosphere and the nu-
cleus.. Stained with Erlich-Biondi no differ
erce is seen between the plasmosphere and
the annulus, both staining deeply and without
any differentiation.
This is by far the most prominent one out
side of the nucleus especially on account o.
the violet color given by the tuloidin blue OIL
by its size. The violet color, however, is MOI
always constant. But this sphere is also dis
tinguished on account of its granulated proto
plasm, which may in most instances be readil,
discerned. This granulation is not regular
but quite uneven, some of the grsnules being