Evening star. [volume] (Washington, D.C.) 1854-1972, 1891-01-15
The Ingredients Of the Curative
Lymph Made Known.
A DESCRIPTION OF Els DISCOVERY
How the Famous German Doctor
Worked Out His Discovery.
GOV. BOYD IN pos SESSION
PROF. koCH's LYMPH.
The Secret Of the rngr~dients Given 20 th
World.


BERLIN. Jan. I5.-The long -taIked-of secret
Of the ingredients entering into the compost
/ ton of rrof. Koch's famous lymph is given to-
day TO the world at large.
Prof. Koch saws: since publishing. two
months ago. the results Of NV experiments with
D the new remedy for tuberculosis many phy-
sicians who received the preparati ON have been
D unable 20 become acquainted with it's prof'~--
/ tee through their own experiments. so far AZ
l have been ahie 10 review the statements pub
| lished and the communications received by letter
| my indications have hc,n fully and completely
confirmed. The general consensus Of opinion
| Is that remedy has a specific effect upon tuler-
| cular tissues. and is. therefore. sppirable ~ Q
very delicate and sure reacent for discovering
latent and diagnosing doubtful tuberculosis
processes
"Regarding the curative effects Of the remedy
most reports agree that. despite the compare
lively short duration OF the application many
patients have shown more or les, pronounced
| Improvement IL has been affirmed that in not
/ few cases even a cure has been established
Standing quite by itself IL the assertion that
the remedy NAV not only be dangerous in cases
! which have advanced too far-a fact which nav
/ forthwith be concrded-but also that IL actu-
aily promoted the tubcrculous process, being
/ therefore injurious.
During the past six weeks I myseif have
mad opportunity 60 bring together further ex-
periences touching the curative effects and
diagnostic application Of the remedy in the
NsaSNH va abbAt oilt nAinftn ana nffN sufferers
from tuberculosis Of the most varied types in
this city and in the Moabit Hospital I can
only say that everything have latterly sen
accords with my previous observation. There
has been nothing 20 modify in what before
reported.
HOW TIE LYMPH WAS DISCOVERED.
Before going into the remedy itself deem
II necessary, for the better understanding of itr
mode of operation, b. state briefly the way by
which i arrived at the discovery. IL n healthy
guinea pig be inoculated with the
pure cultivation Of German kultur
by the inoculstion mostly closes over
with a sticky matter and appears In its early
davs 20 heal Only after ten 20 fourteen days
hard nodule presents itself. which. soon break
ing, forms an uiceratlng sore. which continues
until the animal dies. Quite a different condi-
ton OF things occurs when n guinea Pug al
ready suffering from tuberculosis IL iuoculated.
An animal successfully inoculated from four
to six weeks before Is best adapted for this
purpose. In such animal the small indenia-
tion assumes the same sticky covering at the
beginning. but no nodule forms. On the CON
trary, on the day following or the second day
after the inoculation. the place where the
lynn h Is injected shows a strange change.
1t becomes hard and assumes a darker color
| ing, which Is not confined to the inorulation
spot, bat spreads to the neighbOring parts
until II attains diameter OF from .05 90 o CEN
timeter. In q few days il become more and
necrotic. finally falling of. leaving fiat ulcer
alton. which usually heals rapidly and perma-
nently without any cutting into the djacent
Emphatic glands.
EFFECT OF TSJEcrED TUBERcUfAR BAcuAi.
Thus the injected tubercular bacilli quite
differently affects the skin of a healthy guinea
pig from one affected with tuberculosis. This
effect is not exclusively produced with living
tubercular bacilli, but is also absorbed with the
dead bacilli. the result being the same whether,
as discovered by experiments at
the outset the baeilh are killed by
a somewhat prolonged application OF
a low temperature. or boiling heat or by means
Of certsin chemicals This peculiar fact 1 fol-
lowed up in all directions and this further result
was obtained-that killed pure CIVILIZATIONS of
tubercuSar baccilh. after rinsing in water. might
be injected in great Quantities under healthy
guinea pigs skins without anything occurring
beyond local suppuration."
Prof Koch here interpolates a note that such
| injections belong to the simplest and surexst
means of producing suppurstion free from is-
| ing bacteria.
''Iuberculous guinea pigs, on the other hand,
are killed by the injection Of very small guam
within six to forty-eight hours, accord
ing to the strength of the dose.
An injection which is not sufficient
| to produce the death of the animal NAV cause
extended necrosis to the skin in the vicinity of
| the place of injection. IL the diution is still
further diluted until il is scarcely visibly
clouded, the animals inoculated remain alive
and noticeable Improvement in their condi-
tion soon supervenes. IL the injections
continued at intervals of from one
TO two days the ulcerating inoculation
| wound becomesfsmaller and finally over,
| which otherwise II never does: the size OF the
| swollen lymphatic glands is reduced, the body
| becomes better nourished and the morbid pro-
cess ceases, unless il has gone too far. in which
case the animal perishes from exhaustion. BY
this means the basis of a curative process
| against tuberculosis established
SOLUBLE REMEDY NECESSARY.
Against the practical application Of such
dilutions of dead tubercle bacilli there pre-
sented itself the fact that the tubercle bacili
| are not absorbed at the inoculation points, nor
do they disappear in another way, but for q
long time remain unchanged and greater
smaller suppurstive foci Anything, there
fore, intended TO exercise healing ef-
effect on the tuberculous process
l must be soluble substance which
would be lixiviated to a certain extent by the
| fluids of the body floating around the tubercle
bacilli and be transferred in q fairly rapid
manner to the juices of the body, while
tns suppurssSon Sp
bacilh or dissolyes but very slowly.
The only important point was there
fore, TO induce outside the body the
process going on inside if possible. and to er-
| tract from the tubercular baciih alone the curs-
tive substance. This demanded time and toll,
until finally succeeded with the aid of 40 10 50
per cent solution of glycerine in obtaining
an effective substance from the tubercular
| bacii With the fluid 30 obtained 1 made
| further experiments animals and finally
| human beings. These fluids were given 10
| other physicians to enable them TO repeat the
| experiments. The remedy which is used in
! the new treatment consists Of a glycerine ex-
tract, derived from the pure cultivation a
tubercle bacilli.
| THE REMEDY DESCRIBED.
Into the simple extract there naturally
passes from the tubercular bacilli, besides the
effective substance. all the other matter solu-
BILE i'm fifty per cent glycerine. Consequently
| it contains . certain quantity Of mineral salts,
coloring substances and other unknown ex-
tractiye matter. Some Of these substances
can be removed from IL tolersbly easy.
| The effective substance is insoluble
in absolute alcohol Ii can be precipitated by
it, though not indeed. In a pure condition but
still combined with the other extractive matter
which is likewise insoluble in alcohol The
coloring matter may also be removed, render
ing il possible TO obtain from the extract
colorless, dry substance containing
the effective principle in a much more eoncen-
centrated form than lhe original glycerine solu-
tons For application in practice this purihcs-
ton Of the giycerine extract Offers no sdvsn-
tage, because the substances 30 eliminated are
unessential for the human organism. The pro-
cost OF purihcstion would make the cost of ths