The Daily times. [volume] (Richmond, Va.) 1886-1889, 1888-10-27
THE CHOLERA MfCR0BE.
THE SUCCESSFUL EXPERIMENTS OF
A RUSSIAN PHYSICIAN.

A Method Of Preventing the scourse
ty Inorulation - Immunity from tire
Disease secured by the Use of stosiIizcd
Virus.
The method 81 preventing disease by inocu-
lat'on is. os probably the most .unscientihc
reader knows By this time, % Introduce into
the system n modified form OF the poison that
is the original cause Of them.hdy We rcn-
der The system impcrviotis 10 smallpox 10y
causing 70 pass into tho blood by means Of
incubation q milder form Of the pus which
IL its Characteristic and Which IS obtained
from cows or calves. The hydr6pholia IS
prevented by the same N.Y., also tho cher
Ton ia sheep and the neascs IA hogs. 1t u
not many years since the discovery was made
that the diseases mentioned, with many 9th
ers, were caused by microhc on microscopic
animal that invaded the mouth, throat, lungs,
liver and other internal parts Of tho body,
there Icing different varieties that caused
different diseases
TIE cHoLrRAIC MIcRoDr.
IL is now known that the cholera is caused
by inicroDc, which was discovered by Koch,
aud which he has been cultivating Tor some
years in mild form, with the idea Il using
the Culture to prevent the disease. lie
tried it on animals, and came TO the conclu-
sion that they could not bc inoculated with
useful results 1t now appears from report
just made by Pasteur 10 the French Academy
Of Medicine that physician Of Odcssa-Onc
Of his discipline been more successful.
Koch found the chocraic micrOoo that he
cultivated too feeble 10 affect the system
when INTRODUCED into the blood by inocula-
ton. Only in single instance did the pupil
sent by Pasteur 10 Egypt 20 study the cholera
succeed in giving the cholera 10 a fowl. Or.
Gamaleia, the Russian physician in question,
has discovered the means Of giving ID the
choleraic virus the essential yirulencc which
II lacks. His riethod IS to inocuatc guinea
pig, and with the yir1s 20 inOculate pigeons,
Which are shortly nttacksl and die with the
cholera. The cholera microbc afterwari APP
pears in their blood By inoculating pigeons
succcssivcly witt the virus of one another it
has been found that after the introduction Il
a few drops the last died ia from eight 60
twelve hours, showing all the symptoms Of
cholera. The same virus ia smaller dose
killed guinea pigs ia al claaliy short space
Of time.
With this virus, that had been proved alb
solntely mortal, the experimentalist was able
to establish the existence Of a choieraic i'm
munity. 1t was done in this manner: Two
pigeons were inoculated twice with the ofdin-
airy non-yiruleut cboleraic culture the first
time in the pcctoral muscles, the second tire
in the abdominal cavity. The virulent virus
that had already caused death IN 50 many
cases was introduced into the two pigeons
and hsl no effect whatever. The fact that
human beings can be inocuia;el with the
cholelaic virus and so receive immunity from
the cholera was thus considered to bc estab-
lished.
4 STEJLIZED VIRUS.
The virus Obtained from the treatment Of
q series Of pigeons las 10 be curoIsly treated
10 render 1t innocuOus It is cultivated IN
the ordinary manner in houillon. which IS
afterward heated 60 I'D deg. cenusrade 10
Kill the germs After this beating has been
continued twenty minutes, there still r.
mains toxic principle. Four cubic cent?
meters If 1t introduced into the guinea pig
caused death in from twenty 20 twenty-iour
hours. with absence Of cholcraic microbes ia
the inicstincs a greater quantity las been
found necessary 10 Lii pigeons. II, however,
the virus. 20 the amount If twelve cubic cent?
meters. j. not introduced 40 oncr, but during
a period extending over three or four days,
it docs rot a.ill them aud tLCy Lavc become
refractory Go Tic cholera. II b2s been shogn
that one-half cubic meter Ci the most vlru-
ient virus obtained FROM pigeons can be- il-
troduccd without danger into the system by
incubation The introduction oft"O cubic
ceuumaera into guinea pig, y the operation
extends over two or three seances, IS AT
tended with 80 danger aud gives immunity
from cholera,
Science. therefore. finds iiscll in the pres
ence OF "a practical method OF vaccination Tor
the cholera. This incubation is effected with
q virus that has been ster.lize,.-.Iat is dc
priycd of It's living microbes, instead Of with
one that Is technically called chemical"
DR.. Ganialcia declares that lis remedy IS
without danger ami without exception, so far
rs his experiments on animals are concerned
aud Lo expresses the bencf tia, the time IS
no: tar iii taat when IL wall be applied 70 hu-
man beings 10 preserve them from the Asiatlc
cholera
In a letter to Pasiour. Dr. Gamale.a do
clarcs himself ready to repeat at Paris the
experiments be has made al Odessa, aud 10
quests that the Academy OF Sciences will
appoint tire co4vcn!ent FOR that purpose
Further than this, nc offers u visit the com
tries afflicted with the cholera 70 prove 10y
practical experiment the efficacy OF hg
method. Di. Gamalcia has devoted several
years to tic examination Of the microbes
q a. d
one occa ioa sent 10 Paris b. tho municipality
Of Odessa t. study Pasteurs system Of curing
the hydrophob.a. Francisco Chronicle
Railway SYSTEM ia England.
The specs. II the fastest English trains Is
greater tnan that of the quickest American