The Osceola times., 1886-02-13
THE GREAT DISCOVERY.
An Explanation of Pasteur's Plan
to Check Hydrophobia.
The Way In Which the Virus Is Made aud
Inocuiated - Success OF the Treat
ment-The Difficulty Of Founding
Hydrophobic Hospitals.

All the world knows that four chill
dren from Newark, bitten by mad-dog,
December 2, 1885, were sent to France
TO undergo NJ. Pasteur's treatment
against rabies. Since then another
patient, named Kaufman, has arrived
from America. 1t will be useful
thing to show in a precise manner, ac-
cording to the information gained in
the laboratory of NJ Pasteur, the way
in which people who are bitten are
treated. The simplest method will be
to follow what was done to the four lit
tie children.
These children arrived in Paris, De-
cember q. That same evening they
were brought to the laboratory in the
Rue d'Ulm, and NJ. Pasteur declared
himself ready to inoculate them imme-
diately, Notwithstanding that the inocu-
lations are usually performed by DIR.
Graucher, Professor of the Paris Medi-
cal Faculty, at about eleven o clock the
morning of each day. NJ. Pasteur, not
IaNiiS lthNl illo AOtlti utRitc III
medicine, can not, according to French
law, treat any human malady, can not
perform any medical operation ON
man, and can noteyen legally inoculale
u person
The matter used for incubation Is
composed of veal broth, perfectly pure,
exempt from any microbe, and in which
has been dissolved a little OF the marrow
of a rabid rabbit. The broth and the
marrow must be carefully prepared
The broth is made after well-known
methods with veal which has been boiled
in water. II is then put in glass re-
ceyers of a spherical form. with long
necbs, which are Theoretically sealed
by melting the necks over u lamp.
These receivers are then submitted for
hall an hour under pressure at a heat Of
10 degrees centrigrade (239 degrees
FahrenheiD in a digester somewhat SIM
liar to that of Papin digester and
those used by investigators in physics.
This boiling is intended to entirely de-
stroy all germs the broth may contain.
To make matters more certain the retorts
are left in stove heated TO 80 degrees
centrigrade (95 degrees FahrenheiD.
The germs develop II they have not
been DESTROY ed, and the broth becomes
clouded. When it remains clear one
can dccani il, taking precautions for
excessive cleanliness. into other little re-
torts, known as Paslcur retorts, or in
some similar receptacle.
To get the marrow one must uepan
two rabbits at least each day in the
laboratory. TO do that the animal is
stretched on a dissecting board. TO
which il is firmly attached by IFS four
legs. Then il is chloroformed. ,-One
can tell that il is thoroughly asleep by
its regular respiration, and having lost
its consciousness il can not resist this
regular breathing Ly any jerking
movements. One Sits the skin of the
upper part of the Uranium longitude
nally. One removes, with the help of
a surgical instrument known as the
trepan, small round piece from the
Il skull, taking great care not TO touch
n the membranes of the brain. A man
must have acquired good deal Of skill
through practice to do this. That done,
one inoculatcs with the help of u little
hypodermic syringe, under. the first
membrane, or dura mater, a little OF
the infected broth which one has
had kept for that purpose. enc stitches
together the wound and leaves the
rabbit to recover. On its awakening
u the rabbit seems to be as little troubled
as it did before the operation took
place.
After the inoculation of a very viru-
lent broth rabies declares itself without
fail in the rabbit at the end of six days.
I'll Two tb sla,.a Iat,r ,a amin. nj Htag
q of complete exhaustion or paralysis.
4 Then one takes the marrow, taking
care to let no foreign germ get mixed
4 with it, and hangs itup IN special TASKS
N perfectly sterilized, and containing
caustic potash, to thoroughly dry The
81 air inside Of them. The TASKS are
n placed in room carefully kept up at a
" heat of twenty degrees centigrade (sixty-
e eight degrees fahrenheit.) This ex-
l& posure to a dry atmosphere and con-
M slant temperature is intended TO pro-
# duce a Weakening OF the rabie virus
4 &contained in the marrow. According
M TO the length of time that the, marrow
remains in the dry air for instance, in
Zone, two, up to ten or fifteen days--one
has what NJ Pasteur calls marrow of
%$one, two, ten or fifteen days strength,
and so on, becoming less and less viru-
:|i lent. When one wants TO use it he cuts
|5<on little round piece of about a CEN
timetrc in length, which is mixed with
the broth in a quantity suitable for
Treating four or five persons.
a The first evening each of the Newark
l<children received half a pravas or bypo-
dermic syringe full of broth with mar
low of thirteen davs' STRENGTH An
b dult receives each time three-quarters
1Gpi syringe or whole syringe full.
l'he morning after at eleven o'clock
zach child received half syringe of
pc broth and marrow of twelve days;
nd after the second day they come every
orning toithe laboratory to receive the
oculation. Each day marrow was
|ed which was one day younger. and
eIefore one day stronger. So the
fidren were inoculated ct the end II