Gloucester County Democrat. (Woodbury, N.J.) 1878-1932, 1886-01-14
M. Pasteur Uses Veal Broth in Which
Rabid Rabbits Marrow Has
Been DissoIved-A Pain
less Operation.


marrow must be carefully prepared. The
broth is made, after well knownmethods,
with veal which has been boiled in water.
It is then put in glass receivers, Of
spherical form, with long necks, which
are hermetically sealed BY melting the
necks over lamp. These receivers OF
retorts are then submitted for half an
hour under pressure at a heat of lis de-
grees centigrade (239 degrees Fahrenheit
in digester somewhat similar to a Papin
digester and those nsnd bu ir,vastie-a+ar-
in physics.
This boiling is intended to entirely de-
stroy all germs the broth may contain
To make matters more certain the retorts
are left in stove heated to 35 degrees
centigrade (ninety five degrees Farenheit)
The germs develop if they have not been
destroyed, and the broth becomes
clouded. When it remains clear one
can decant it, taking precautions for ex-
cessive cleanliness, into other little re-
ceptaeles, known as Pasteur receptacles,
or into some similar receptacle.
To get the marrow one must trepan
two rabbits at least each day in the labor
atory. To do that the animal is stretched
on dissecting board, to which it is
firmly attached by its four legs. Then it
is chloroformed. You can tell if it is
thoroughly asleep by its regular respira-
ton, and having lost its consciousness it
cannot resist this regular breathing by
any jerking movements The skin Of
the upper part Of the Uranium is slit
longitudinaHy Then with the help of
surgical instrument known as the tre--
pan smallround piece is removed from
the skull, great care being taken not to
touch the membranes of the brain. A
man must have acquired good deal Of
That done, Tne operator InUcuIaJes wITn
the help of little Pravas, orhypodermic
syringe, under the first membrane, or
dura mater, little Of the infected broth
reserved for the purpose. He stitches
together the wound and leaves the rabbit
to recover. On its awakening the rabbit
seems to be as little troubled as it did
before the operation took place. After
the inoculation Of very virulent broth
rabies declares itself without fail in the
rabbit at the end of six days. Two or
three days later the animal dies Of com
plete exhaustion Or paralysis
Then the marrow, care having been
taken to let no foreign germ get mixed
with it, is hung up in special TASKS per-
fectly sterilized, and containing caustic
potash, to thoroughly dry the air inside
of them. The TASKS are placed in room
carefully kept up at heat Of twenty de-
grees centigrade (68 degrees Fahrenheit).
This exposure to dry atmosphere and
constant temperature is intended to pro-
duce weakening of the rabic virus con-
tanned in the marrow according to the
length of time that the marrow remains
in the dry air. For instance, in one, two
up to ten or fifteen days, one has what M.
Pasteur calls s'marrow of one, two, ten
or fifteen days strength" and so on, be-
coming less and less virulent. When the
operator wants to use it he cuts off a
little round piece Of about a centimetre
in length, which is mixed with the broth
in quantity suitable for treating four or
five persons.
The first evening each Newark child re-
ceived half Pravas or hypodermic syringe
of broth with marrow of thirteen days
strength. An adult receives each time
three-quarters Of a syringe Or whole
syringe full. The morning after, at II
o'clock, each child received half a syringe
of the broth and marrow Of twelve days,
and after the second day they came every
morning to the laboratory to receive the
inoculation. Each day we take marrow
which is one day younger, and therefore
one day stronger than that used the day
before. So the children arrived at being
inoculated at the end of about ten or
eleven days with marrow of from one to
two days strength, which is certain to
| give rabbit rabies by trepanning at the
end Of six days. 1t will do no harm to
the children, and, on the contrary, will
| cure them, for the inoculations that pre-
ceded prepared them to receive the most
yirulent marrow without danger.
The first marrow of thirteen days
would not kill rabbit. That of six OF
seven days kills them at the end Of ff-
teen days and does not affect the child.
The treatment lasts about ten days.
The inoculation causes no pain beyond
that produced by the pricking of the
very fine needle which is inserted in the
skin in the side, one day on on the right,
another day on the left. At the end q
few days the children bear the PricIing
even without crying. There is no in
hammation nor redness, unless it be OF
the last day, after the inoculation Of thu
most virulent marrow; but there is M
pain even then. During treatment thl
patients can 20 out and do what the.
please It is enough that they shoult
avoid excesses.
! M. Pasteur's method consists in inoeu