Vermont phÅ“nix. [volume] (Brattleboro, Vt.) 1834-1955, 1886-01-08
NJ. PASTEURs PROCESS.
The Method Employed In his Hsen
NnOculnttOn,

(Cable despatch to Boston Heraldl
II will be a useful thing to show in = pre-
else manner, according to the informetion
gained In the laboratory Of M. Pasteur, the
way in which people who have been bitten ny
mad dogs are trested. The simplest method
will be to follow what has been done % the
four Newark children, who arrived in Paris
Dec, 81. That same evening they we're taken
to the laboratory in the rue dUlm, and NJ.
Pasteur declared himself ready TO inoculate
them Immediately, notwithstanding that lhe
inoculstions are usually performed by d..
Groucher professor Of the Paris medical fac
ulty, at about l / o'clock on the morning of
each day NJ. Pasteur not having taken bis
doctors degree in medicine, cannot, accord
ing to the French law, treat any human mai
ady cannot perform any medical operation
on q man, and cannot, legally, even inocplnte
person. The matter used for inoculation b
composed OF veal BROTH perfectly pure, ex
empt from any microbe, and in which haB
been dissolved little OF the marrow Of fhb-
id rabbit The broth and the marrow must
bs carefully prepared. The broth is made
after a well-known method, with veal which
has been boiled IN water. II is then put M
glass receivers of spherical form, with long
necks, which are hermetically sealed by melt
ing the neoks over a lamp. These receivers
or retorts are then submitted for half an hour
under pressure at a heat Of 115o centigfade
(239' Fahr y in digester somewhat similar
to that of Papin digester and those used b.
Investigators in physics. This boiling is nj
tended to entirely destroy all germs tue broth
may contain. To make matters more oeT-
tain, the retorts are left in a stove heated to
30 centigrade (95' Fshr }. The germs DO.
velop il they have not been destroyed, aud
the broth becomes clouded. When 1t remains
clear one can decant it, taking precautions for
excessive cleanliness, iuto other little retGFia,
known AZ Pasteur retOrts, or in some similar
receptacles. To get the marrow, one must
trepsn two rabbits, ut least, each day in the
laboratory. To do this, the animal is stretch
ed on a dissecting board, to which it is firmly
attached by its four legs. Then it is chloro
formed One can tell that it is thoroughly
asleep by its regular respiration, and having
lost its consciousness 1t cannot resist this reg
ular breathing by any jerking movement
The operator Sits the skin OF the upper par
of the Uranium longitudnally, and removes
with the aid Of a surgical instrument knowT
as the trepsn, small round piece from n,
skull, taking great care not to touch th'
| membranes OF the brain. man must havl
acquired good deal Of skill through practice
| to do this. This done, the operator inocu
lstes, with the help OF a little pravas OF hypo
dermic syringe, little Of the injected brotl
which has been kept for the purpose. H
then stitches together the wound, and leave
the rabbit to recover. On its awakening, %
rabbit seems to be AZ little troubled al befor
the operation took place. After the inocula
ton OF very virulent broth, rabies declare
itself without fail in the rabbit at the end o
six days. Two or three days later the anim1
dies of complete exhaustion or paralyais
Then the operator takes the marrow, takini
care to let no foreign germ get mixed with Il
and hangs !. up in special TASKS, perfectl
sterilized aud containing caustic potash ;
thoroughly dry the air inside. The TASKS O.
placed in room carefully kept up at hes
OF 20 csntigrade (Gs Fahr.). This exposul
3. 10 dry 'osphere aud constant tempera