Memphis daily appeal. [volume] (Memphis, Tenn.) 1847-1886, 1883-11-17
YELLOW-FEVER.
Some Wonderful Experiments Conducted
4 After Thoroughly Scientific Meth.
DDS at Rio Jan, irO.
BY Which the COntagion and Transmits.
sion Of the Disease Was Proven
Four Successive Times.

metro, showing the transmission of yellow
fever by contagion, was forwarded to the
Department of State by Minister An
drews: On the 14th instant we took from
the heart of a person who died of yellow
fever an hour before, some grams of blood,
in which the microscope revealed the
presence of the cryptococci that are now
currently considered to constitute the
characteristic of that disease. Those
organisms were in different phases of full
development, from the size OF small black
points to that of large round cells, grayish
or dark, fringed with billiant point in
the center Besides these there were to
be seen masses of transparent granula-
lions, set in gauge of yellow pigment.
We took one gram of the blood, and, with
every care that the case required, our able
assistant, Sr. AIenezes Doria, made an ex-
traneous injection in the great vein of a
limb of a rabbit. Fifteen minutes later
tetaniform convulsions showed themselves,
with back hollowings, and the animal
soon succumbed, Illuminated, so to say, by
the violence of the virus too rapidly and
directly introduced into the great circu-
latory torrent. At first we suspected that
some accident had caused the death of the
animal, such as air getting into the vein,
or some clot, but the operation was exc
cuted with the utmost care; and
the death in these cases should
have been sudden and unaccom-
panied with the course of symp-
toms just mentioned. ON making the
autopsy we found visceral congestions
analogous to those that we had seen i'm the
corpses of yellow-fever patients, and we
found in the blood the same cryptococci
as existed in the corpse that served for the
incubation. If the death had been due
to the quick action of the cryptococci, and
not to an accident, the blood of the rabbit
should, when itself inoculated in another
animal, cause the death of the latter. To
put this beyond doubt, we took a gram OF
the dead rabbits blood, and injected il
hypodermically into the guinea-pig. Well
the latter died at the end of some hours
lin the night of the 14th) and we found
an extraordinary quantity of cryptococc
in its blood, and saw also the anatomo
oathologic LESSONS which usually charac
terize cases of yellow-fever in man. The
death of the second animal was evidently
due to the contagion, and showed thai
the rabbit whose blood was inoculateC
contained in itself the transmittory virus
and succumbed to the influence of thi.
virus. Not content with this, we injected
gram of the guniea-pie's blood under
the skin OF another guinea-pig, and in
the space of some hours this one ap
peared feverish, oppressed with colo
ears and paws; trembling and rejectin;
biacklsh OBJECTIONS. A drop of thi,
anlmars blood showed an infinity o.
cryptococci, and within little time i
also dled; by these experiments we have
proved, therefore, contagion and transmits
SION % OF the disease tour successive times
Inc sick man received II from the medium
III Whlch ne hVed; from MN we passed /
10 the rapDlt, and from 1t to guinea-pig
which, IN turn, transmitted it to anothe
guinea,pig. In all four cases the blooC
showed swarms oil cryptococci. In thi
manner we are aple 10 produce epizootic
IN the animals IN our laboratory by inocu
latlnS many at once with microbiatet
blood 1hese facts, therefore, prove be
yono douDt tnat yellow-fever is propagatet
hy contagion from individual to individual
tnat IL Is prlmluVely contagious disease
but may become infectious as soon as suffi
cientiy many focuses accumulate. The
also prove that the disease does not resid
| excluslVeiy or especially IN any one organ
1t resides In the blood, and, therefore, il
all the organs the blood trayerses.
DIR. Domingos Freire also considers tha
they also establish the parasitic nature O
s..ss ye,iSw fever; and & that this parasite is tn
| fo,,o, o,,J i l. y
oa9o pi yeno" ~, non.;r,.,nS ~ nm III,
~ Aam.nogehieus .,,a d;soornr1 L;, r,eile ans
I ~; ioU4vs 9y nm illili an lsG
ia'Ioi. VI ;ah :. - IL I'm On. oiac a
vi. nT.iili' IN "nIon H. hos rn.sn ~ sIe s.ate VI
e eai' he ease ~. E A' 20 ,aosot; il W ueneN
.hat ~ is PIouiu;G' " :eSiI'ioii or eAGre 3
uol ol llie nilcIopii, 1 ~ mmicl, however, zoe
not preNent potn tlie alsaiolu .I anu tne nll
IIooluS pelns iaotois 9i 'iie inoIpio staIe
II Is Ilqulu, Oh an aclu, aromaIic smell
oIy, lornls an opaiesceh, eIlulsloh VIII
water, ana Is soluble IN aicoloi ana ethei
II strongiy piues Iea nlihus paper, am
must contaln good PropoItion OF nitro
gen, as II glves OUT apunuant % alnm9niaca
NaPoIs wneh hed!eu ml.l po.ash. ne ans
ascer.aliieu oy uiIeo. eApSr,llehl, "ie1 py GU
t1Nalihs cIyP.ocoJcus ti Seia'ine Witiilh
glope, that t1e GoloI 1i p.dch ta Nolilt Is mo
que 10 altere4 pIoou, 4uI the GIyPoococcus
anu aI ne was mus eiaoieu 70 OBTAIN n an artl
tnia iIla, oiacN . oohn.. "a a; ou,.uie VI .ls earli
IaAen, as Lepor!eu III our ISSUE ol apr