Tetraodontidae

Tetraodontidaeis afamilyof primarily marine andestuarine fishof theorderTetraodontiformes. The family includes many familiar species variously calledpufferfish,puffers,balloonfish,blowfish,blowers,blowies,bubblefish,globefish,swellfish,toadfish,toadies,toadle,honey toads,sugar toads, andsea squab.They aremorphologicallysimilar to the closely relatedporcupinefish, which have large external spines (unlike the thinner, hidden spines of the Tetraodontidae, which are only visible when the fish have puffed up).
The majority of pufferfish species aretoxic, with some among the most poisonousvertebratesin the world. In certain species, the internal organs, such as the liver, and sometimes the skin, containtetrodotoxin, and are highly toxic to most animals when eaten; nevertheless, the meat of some species is considereda delicacyinJapan(as 河豚, pronouncedfugu),Korea(as 복,bok, or 복어,bogeo), andChina(as 河豚,hétún) when prepared by specially trained chefs who know which part is safe to eat and in what quantity. Other pufferfish species with nontoxic flesh, such as thenorthern puffer,Sphoeroides maculatus, ofChesapeake Bay,are considered a delicacy elsewhere.
The speciesTorquigener albomaculosuswas described byDavid Attenboroughas "the greatest artist of the animal kingdom" due to the males' unique habit of wooing females bycreating nests in sandcomposed of complex geometric designs.
The family name comes from the name of its type genusTetraodon;it is traced from the Greek wordsτετραtetrameaning "four" andὀδούςodoúsmeaning "teeth".
The Tetraodontidae contains 193to 206species of puffers in 27or 28 genera:
Pufferfish are typically small to medium in size, although a few species such as theMbu pufferfishcan reach lengths greater than 50 cm (20 in).
Tetraodontiformes, or pufferfish, are most significantly characterized by the beak-like four teeth – hence the name combining the Greek terms "tetra" for four and "odous" for tooth.Each of the top and bottom arches is fused together with a visible midsagittal demarcation, which are used to break apart and consume smallcrustaceans. The lack of ribs, a pelvis, and pelvic fins are also unique to pufferfish. The notably missing bone and fin features are due to the pufferfish' specialized defense mechanism, expanding by sucking in water through an oral cavity.
Pufferfish can also have many varied structures ofcaltrop-like dermal spines, which account for the replacement of typical fish scales, and can range in coverage extent from the entire body, to leaving the frontal surface empty.Tetraodontidae typically have smaller spines than the sister familyDiodontidae, with some spines not being visible until inflation.
They are most diverse in thetropics, relatively uncommon in thetemperate zone, and completely absent fromcold waters.
Most pufferfish species live in marine orbrackishwaters, but some can enter fresh water.  About 35 species spend their entire lifecycles in fresh water. These freshwater species are found in disjunct tropical regions of South America (Colomesus asellusandColomesus tocantinensis), Africa (sixTetraodonspecies), and Southeast Asia (Auriglobus,Carinotetraodon,Dichotomyctere,LeiodonandPao).
The puffer's unique and distinctive natural defenses help compensate for its slow locomotion. It moves by combiningpectoral,dorsal,anal, andcaudal finmotions. This makes it highly maneuverable, but very slow, so a comparatively easy predation target.  Its tail fin is mainly used as a rudder, but it can be used for a sudden evasive burst of speed that shows none of the care and precision of its usual movements.The puffer's excellent eyesight, combined with this speed burst, is the first and most important defense against predators.
The pufferfish's secondary defense mechanism, used if successfully pursued, is to fill its extremely elasticstomachwith water (or air when outside the water) until it is much larger and almostsphericalin shape.  Even if they are not visible when the puffer is not inflated, all puffers have pointed spines, so a hungry predator may suddenly find itself facing an unpalatable, pointy ball rather than a slow, easy meal.  Predators that do not heed this warning (or are "lucky" enough to catch the puffer suddenly, before or during inflation) may die from choking, and predators that do manage to swallow the puffer may find their stomachs full oftetrodotoxin(TTX), making puffers an unpleasant, possibly lethal, choice of prey. Thisneurotoxinis found primarily in theovariesandliver, although smaller amounts exist in theintestinesandskin, as well as trace amounts in muscle.  It does not always have a lethal effect on large predators, such as sharks, but it can kill humans.
Larval pufferfish arechemically defendedby the presence of TTX on the surface of skin, which causes predators to spit them out.
Not all puffers are necessarily poisonous; the flesh of thenorthern pufferis not toxic (a level of poison can be found in itsviscera) and it is considered a delicacy in North America.Toxin level varies widely even in fish that are poisonous. A puffer's neurotoxin is not necessarily as toxic to other animals as it is to humans, and puffers are eaten routinely by some species of fish, such aslizardfishandsharks.
Puffers are able to move their eyes independently, and many species can change the color or intensity of their patterns in response to environmental changes. In these respects, they are somewhat similar to the terrestrialchameleon. Although most puffers are drab, many have bright colors and distinctive markings,and make no attempt to hide from predators. This is likely an example ofhonestly signaledaposematism.
Dolphinshave been filmed expertly handling pufferfish amongst themselves in an apparent attempt to get intoxicated or enter a trance-like state.
Many marine puffers have apelagic, or open-ocean, life stage. Spawning occurs after males slowly push females to the water surface or join females already present. The eggs are spherical and buoyant. Hatching occurs after roughly four days. The fry are tiny, but under magnification have a shape usually reminiscent of a pufferfish.  They have a functional mouth and eyes, and must eat within a few days.  Brackish-water puffers may breed in bays in a manner similar to marine species, or may breed more similarly to the freshwater species, in cases where they have moved far enough upriver.
Reproduction in freshwater species varies quite a bit. Thedwarf pufferscourt with males following females, possibly displaying the crests and keels unique to this subgroup of species.  After the female accepts his advances, she will lead the male into plants or another form of cover, where she can release eggs for fertilization. The male may help her by rubbing against her side.  This has been observed in captivity, and they are the only commonly captive-spawned puffer species.
Target-group puffers have also been spawned in aquaria, and follow a similar courting behavior, minus the crest/keel display. Eggs are laid, though, on a flat piece of slate or other smooth, hard material, to which they adhere. The male will guard them until they hatch, carefully blowing water over them regularly to keep the eggs healthy. His parenting is finished when the young hatch and the fry are on their own.
In 2012, males of the speciesTorquigener albomaculosuswere documented while carving large and complex geometric, circular structures in the seabed sand inAmami Ōshima, Japan. The structures serve to attract females and to provide a safe place for them to lay their eggs.
Information on breeding of specific species is very limited.T. nigroviridis, the green-spotted puffer, has recently been spawned artificially under captive conditions. It is believed to spawn in bays in a similar manner to saltwater species, as their sperm was found to be motile only at full marine salinities, but wild breeding has never been observed.Xenopterus naritushas been reported to be the first bred artificially in Sarawak, Northwestern Borneo, in June 2016, and the main purpose was for development of aquaculture of the species.
Pufferfish diets can vary depending on their environment. Traditionally, their diet consists mostly of algae and small invertebrates. They can survive on a completely vegetarian diet if their environment is lacking resources, but prefer an omnivorous food selection. Larger species of pufferfish are able to use their beak-like front teeth to break open clams, mussels, and other shellfish. Some species of pufferfish have also been known to enact various hunting techniques ranging from ambush to open-water hunting.
The tetraodontids have been estimated to have diverged fromdiodontidsbetween 89 and 138 million years ago. The four major clades diverged during theCretaceousbetween 80 and 101 million years ago.  The oldest known pufferfish genus isEotetraodon, from theLutetianepoch of MiddleEoceneEurope, with fossils found inMonte Bolcaand theCaucasus Mountains.  The Monte Bolca species,E. pygmaeus, coexisted with several other tetraodontiforms, including an extinct species of diodontid, primitiveboxfish(ProaracanaandEolactoria), and other, totally extinct forms, such asZignoichthysand thespinacanthids.The extinct genus,Archaeotetraodonis known fromMiocene-aged fossils from Europe.
Pufferfish can be lethal if not served properly. Puffer poisoning usually results from consumption of incorrectly prepared puffer soup,fugu chiri, or occasionally from raw puffer meat,sashimi fugu. Whilechiriis much more likely to cause death,sashimi fuguoften causes intoxication, light-headedness, and numbness of the lips.Pufferfish tetrodotoxin deadens the tongue and lips, and induces dizziness and vomiting, followed by numbness and prickling over the body, rapid heart rate, decreasedblood pressure, and muscleparalysis. The toxin paralyzes thediaphragmmuscle and stops the person who has ingested it from breathing. People who live longer than 24 hours typically survive, although possibly after acomalasting several days.
The source of tetrodotoxin in puffers has been a matter of debate,but it is increasingly accepted that bacteria in the fish's intestinal tract are the source.
Saxitoxin, the cause ofparalytic shellfish poisoningandred tide, can also be found in certain puffers.
In September 2012, theBureau of Fisheries and Aquatic Resourcesin thePhilippinesissued a warning not to eat puffer fish, after local fishermen died upon consuming puffer fish for dinner. The warning indicated that puffer fish toxin is 100 times more potent thancyanide.
Pufferfish, calledpakapaoinThailand, are usually consumed by mistake.  They are often cheaper than other fish, and because they contain inconsistent levels of toxins between fish and season, there is little awareness or monitoring of the danger. Consumers are regularly hospitalized and some even die from the poisoning.
Cases of neurological symptoms, including numbness and tingling of the lips and mouth, have been reported to rise after the consumption of puffers caught in the area ofTitusville, Florida, US. The symptoms generally resolve within hours to days, although one affected individual requiredintubationfor 72 hours.As a result, Florida banned the harvesting of puffers from certain bodies of water.
Treatment is mainly supportive and consists of intestinal decontamination withgastric lavageandactivated charcoal, and life-support until the toxin is metabolized. Case reports suggestanticholinesterasessuch asedrophoniummay be effective.