Document: NRC Regulatory Guide
Document ID: 535957c7-1203-410d-b709-8e4d381f00de
Document Type: regulatory_guide
Title: Measurements of Radionuclides in the Environment, Analysis of I-131 in Milk
Source: NRC Regulatory Guide Division 4
Source URL: https://www.nrc.gov/docs/ML1335/ML13350A246.pdf
Revision Date: 2023-06
Chapter: 
Section ID: RG-4.3
CFR Part: 
CFR Title: 

Content:
ml -dissolve 1.181 grams Nal in 100 ml water 2. Dowex I x 8. 20.50 mesh. Cl" form 3. 2M NaCI . dissolve 116.9 grams NaCI in I liter water 4. NaOCl, 5-6% (commercial strength) 5. HNO.t, concentrated 6. Hydroxylamine hydrochloride, crystals 7. CCI4 8. IM NaHSO1 - dissolve 1.041 grams of NaHSO 3 in 10 ml water (prepare daily) 9. Palladous chloride, 10 mg/ml -dissolve 1.66 grams ofPdCl 2 in 100 nil of water 4.3.3 a.,: Procedure 1. Prepare an ion exchange column 2 cm in diameter by 10 cm long and fill to a height of 5 cm with Dowex I x 8. 20-50 mesh. CI" form. Add the resin from a water slurry. 2. Add 1-2 ml of iodine carrier (10 mg 1'/ml) to 4-liter .. ,ilk sample and stir thoroughly. Accurately measure and record exact volume of carrier added. * Pass through the column at a flow rate of about 30 nml per minute and discard effluent. 3. Wash the column with 500 ml hot (500C) distilled water followed by .100 ml of 2M NaCI. Discard washes. 4. Transfer the resin to a 250-ml beaker using 50 ml of 5-6% NaOCI. (Note 1) .5. Place a small ("2 cm long) magnetic st,.ring bar in * the beaker and stir vigorously for 5 minuies on a magnetic stirrer. 6. Filter the resin slurry. through a suction filter and retain the NaOCI solution. (Note 2) 7. Reextract the resin by repeating steps 4. 5. and 6. 8. Discard the resin, combine the two 50-ml solutions, and carefully add 20 ml or conc. H ,N0 3. (Note 3) 9. Pour the acidified NaOCI solution into a 250-ml * separatory funnel and add 50 ml of CCI4. 10. Add 1.5 gm of hydroxylamine hydrochloride and shake (Note 4). Extract the iodine into the organic phase (about 2 min. equilibration). (Note 5) l 1. Drain lower organic, phase into a clean 250-ml . .separatory funnel and save. 12. Add 50 ml CC14 and I gm hydroxylamine hydrochloride to the aqueous phase in the first separatory funnel and reextract. Combine organic phases and discard aqueous phase. , 13. Add 25 ml H20 and 10 drops of freshly prepared IM NaHSO 3 to the separatory funnelcontaining