Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 periments. By means of designed program the effects of psychological suggestion of the sub- jects were prevented. An imitator group was included that the subjects received the imitator “gi” from other people. The spectrum analysis of EEG was done by a computer on line. Two samples were made before emitting qi and averaged. The results showed that when subjects receiving qi and four were made emitting qi, the changes of EEG took place mainly in the beta frequency segment ,and the power of beta seg- ment was higher than that of the control. The increases were statistically significant if Fp2, C3,C4,Ol1,and Pz, five points of betal segment and.in C3 and C4,two points of beta2 seg- ment ,P<0. 05. The power of EEG of the qigong master in the beta segment also increased when he was emitting gi,and apart from C4 and Pz points in beta | and C4 point in betaZ seg- ment, the increases in all other points were statistically segment. In the imitator group ,there was no obvious change in the power of EEG in the beta segment when the subjects received qi from other people imitating qigong masters. Similar changes of power in the beta segment of EEG that took place in both the qigong master while emitting qi and subjects when receiving qi suggested that the qigong master had somehow influenced EEG of subjects . The mechanism and significance of these phenomena are unclear yet and further studies are needed. 1—36 QIGONG'S SUGGESTIVE EFFECT SEEN IN EEG Kimiko Kawano Information Processing Center for Medical Sciences, Nippon Medical School, Bunkyo-ku, Tokyo 113 Qigong has been already well-known in Japan. The effect of the emitted qi and hypnosis are caused by suggestion. Sixteen channels of electroencephalograms (EEGs) during the e- mission of qi and hypnosis were studied. Three Chinese qigong masters. and three healthy Japanese subjects and a Japanese hypnotist and six receivers worked together. A double-blind test was adopted,so the qi-receivers,as well as the researchers, could not know the time when the qigong masters emitted their qi. I have previously reported that during the emission of qi (1) The a waves of the qigong masters appeared on the frontal area. (2) Those frontal « waves synchronized with the occipi- tal a. (3)The B waves showed the specific pattern on the topography ,and (4) These changes in the EEGs of the qigong masters appeared as similar changes in the EEGs of their subjects. Under the double-blind test conditions ,no clear synchronized changes were found in the « 99 « . Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 B waves . However ,the effects of (1) and (2) were observed clearly in all receivers. During hypnosis led by means of non-verbal method ,the 8 waves of the subjects still appeared on the linguistic area. These results indicate that hypnosis is the verbal suggestion at all, while qi is not a so-called placebo, but something that can be transmitted transpersonally. 1—37 THE EFFECT OF QIGONG ON EXPERIMENTAL IN- FLAMMATION AND NEUTROPHIL CHEMILUML NESCENCE | Yan Yizhao,Chen Xiangyin Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences , Bei- jing ,100005 In order to evaluate the possible role of qigong in treatment of some inflammatory dis- eases , the effect of qigong on the experimental inflammation (paw edema) and neutrophil (PMNs) chemiluminescence (CL) were studied. ‘Wistar rats ,weighing 200— 250g were used in this investigation. The paw edema was produced by an injection of 0. lml of a 1% suspension of carrageenin in 0.9% saline into the hind| paw. The length round the paw was determined six hours later. PMNs were isolated from the peritoneal cavity of glycogen treated rat. The CL system consists of 5X10°PMNs, 0. Iml of 0. 1mM luminal and lmg of opsonized zymosan . The CL reading was determined by a Biological Chemiluminescence Instrument. The results showed that (1) Qigong could inhibit carrageenin-induced paw edema. The increased length round the paw CILRp) in the qigong group was 0. 557-E0. 035em(n=26,P <0. 01),and was similar to the hydrocortisone group (0. 206+0. 028,n—26) (2) Qigong could stimulate PMNs CL in vitro. The CL reading in the qigong group increased from 168. 74£16.2 (n=22)to 236. 3423.5 (n=22,P<0.01). . Neutrophil (PMNs) is an important inflammatory cell. PMNs activation and products (lysosome enzymes and oxygen free radical) may play an important role in the pathogenesis of acute inflammatory reaction. In the present study,we have found that qigong can prevent the development of carrageenin-induced paw edema and stimulate PMNs activation in vitro. | The possible mechanism of the qigong effect is very complex and will be studied in future. 1—38 PROTECTIVE EFFECT OF THE EMITTED QI ON THE PRIMARY CULTURE OF NEUROCYTES IN VITRO A- GAINST FREE RADICAL DAMAGE Tang Yipeng,Sun Chenglin, Hong Qingtao,Liu Chunmei,,Li Xiaoming Institute of Qigong Science, Beijing College of T.C. M. Beijing 100029,China This paper reported the protective effects of the emitted qi on cultured neurons damaged Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 by free radical . The neuronal cell’s suspension was made with the cerebral cortex of Wistar O-day newborn rats and it was cultured in dishes. At 8th--day postplating,the dishes were divided into 3 groups,i.e. the emitted qi group,damaged group and normal control group. The neurons of the first two groups were injured by hydroxyl free radical generated in the ascorbic acid system. We observed that the brightness of the somatic margin in the damaged group turned from bright to dark,the volume of neuronal somata increased, the number of neissl boby decreased, the effusion of LDH increased and GSH-Px activity changed obvious- ly. These evidences indicated the neurons in the damaged group had swelling or degenerat- ing. But in the emitted qi group, the effusion of LDH was less than that of the damaged group. Both groups showed an obvious difference (P<. 0. 01-0. 001), GSH-Px activity changed a little. The results show that the emitted gi may act as a scavenger of hydroxy radi- cal and protect the nerve cell membrane. 1—39 THE EFFECT OF THE HUMAN ENERGY FIELD UNDER THE QIGONG STATE ON THE STRUCTURE AND FUNC- TION OF RED BLOOD CELLS IN EXPERIMENTAL RATS WITH BLOOD STASIS Liu Chunmei,Sun Chengling Beijing College of Traditional Chinese Medicine , Beijing 100029 ,China Qigong is newly recognized by people as a modern medical means to be used for prolong- ing life and enhancing the human immune function. Especially, it has a particular action for treating chronic, difficult and complicated cases. The action of drugs on organic microcircu- lation was reported before in medical literature, but the effect of the human energy field un- der the qigong state on the structure and function of the erythrocyte membrane has not been reported yet. Through the investigations of the human energy field phenomenon, Inyushin put forward an energy stroma model about the human energy field according to many books written by researchers. This stroma model constitutes the stroma structure which can make organisms exist. Some methods developed from this energy stroma model are now used in medical. treatments. The experiments made by our office before have proved that the human energy field under the qigong state can improve blood rheological property of rats with blood stasis. Researching results show that the human energy field: under the qigong state has the action of protection of red blood cell structure and function in animals with blood stasis ,and that the content of red blood cells and peroxide lipid of liver tissue can be reduced , sialic acid content of the erythrocyte membrane can be recovered. Thus, it follows that the human ener- gy field under the qigong state can relieve or prevent organism cells from free radical damage and that the human energy field released by qigong doctors can,to some extent regulate dis- ordered organism. But the protection of membrane protein structure damage is not good. We * 101° Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
™ Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 have used animal models with blood stasis to observe the change of symptom due to blood stasis ,and then to observe the action of the human energy field under the qigong state on red blood cell membrane, and finally to research into the mechanism of the human energy field regulating organic metabolism. 1— 40 ANALYSIS OF THE EFFECT OF THE EMITTED QI ON HUMAN HEPATOCARCINOMA CELL (BEL-7402) BY USING FLOW CYTOMETRY Chen Yuanfeng Shanghai Institute of Traditional Chinese Medical Science ,Shanghai 200030,China Based on our previous studies of the cellular effect of the emitted qi on human hepatocar- cinoma cell (BEL-7402) in this experiment, the state-of-the-art technique of flow cytometry (FCM) was employed to analyse the influence of it on BEL- 7402 from the angles of cell divi-- sion, kinetics and genetics, Repeated reults indicated that the emitted qi treatment inhibited the higher rate of DNA synthesis in BEL-7402 and decreased the concentration of DNA . Meanwhile ,the cell cycle of BEL- 7402 was correspondingly altered. GO,G1,G2 phases became more obvious by repeated treatments »but S phase appeared to be smaller. After the emitted qi treatment chromosome | karyotype changed too . These results demonstrated that the BEL-7402 cell could be restored | to the normal to some extent by qigong action. For the further confirmation, naked mice was inoculated with BEL-7402 and the treated cell respectively ,and it was surprisingly observed that the tumour was only induced in the naked mice inoculated with BEL-7402, whereas naked mice with gigong treated BEL-7402 grew well. This result was further evidenced by the FCM analysis of cells isolated from in situ tissue. 1—41 THE EFFECT OF THE EMITTED QI ON AGGLUTINAT- | __ ING REACTION OF HUMAN PULMONARY ADENOCAR- CINOMA CELL (SPC-A1) MEDIATED BY CONA Chen Yuanfeng Shanghai Institute of Traditional Chinese Medical Science »Shanghai 200030, Chi- na Intercellular recognition and contact inhibition are the main characteristics of the cultured an- imal cell »which usually appear as agglutinating reaction mediated by some plant lectins, such as ConA. It has been proved that there is close relationship between the agglutination and glycoproteins on cell membranes. The sort and distribution of glycoprotein as well as its in- teraction with relative receptors can influence the agglutinating reaction. In general normal cells expresses less degree of agglutination than tumour cells. Therefore sin this experiment, we used SPC-A1 cells as the material,and the effect of the emitted qi on cell recognition me- . 102 . Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6