‘Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 The use of cultured cells, separated from the body and therefore isolated (at least to a certain degree) from the influences of the mind offer a unique experimental approach to understanding the mechanism of action of scalar energy in the healing process. This approach could he used to determine direct effect of scalar energy on cells fram either the brain or the body. Tissue culture cells are a stable, controlable biological preparation which could also be used to study the biochemical mechanism of action of scalar energy. Even if scalar energy directly effects individual cells, it may also effect the mind resulting in an altered psychological outlook which results in clinical improvement. Dr. Persinger was the first person to test the effects of scalar energy using isolated cells(17). The scalar waves were generated by partially cancelling two vortex-type magnetic fields(0.5Hz, 10uT) by intersecting them in air. Although a scalar field is likely to be generated in this protecol, it was not measured. The biological endpoint, degranulation of mast cells, was increased by the scalar fields. It is unknown to what extent the EM fields contribute to.this result. . Very recently, Dr.Puharich has reported that the scalar. energy from the Tesla watch effects the DNA of E.Coli cells grow in tissue culture(18). Working with colleagues at the Max Planck Institute in Germany, they have increased activity of ubiquitone. Ubiquitone is a protein involved in DNA repair. These results indicate that scalar energy can have a direct effect at the subcellular level as well as a direct effect on the immme system > EFFECT OF SCAIAR ENERGY ON NEUROTRANSMITTIER FUNCTION She present study is the first to report a direct effect of scalar energy on nerve cells in tissue culture and indicates that scalar energy can modulate the basic biochemical couimunication between nerve cells offer a unique model to answer this question. A particular type of nerve cell, called PC12 cells, originally isolated froma rat adrenal phaeochramecytoma, was used because its neurotransmitter properties have been well characterized and shown to be similar to, those occuring during normal synaptic transmission in the brain (19,20). Furthermore, the author has previously shown that the functional properties of noradrenaline, an abundent neurotransmitter in these cells is altered by weak EM fields. Noradrenaline release from PC12 cells was shown to be increased by a 500Hz monopolar square wave(21) and noradrenaline uptake was inhibited by a 15 Hz bipolar square wave (22). ; Noradrenaline uptake was chosen as the functional neurotransmitter critical cellular barrier which interfaces with the chemical and electromagnetic environment. Since other intramembrane proteins show - non-linear properties, the plasma membrane appears to be a likely target for scalar energy. Although neurotransmitter release also involves the plasma menbrane, it is a mich mre camplicated process which occurs several biochemical steps down the line from the first exposure of the membrane to the EM signal. -3- Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9
Te release reve excess neurotransuitter from the synaptic elect after its release fron presynaptic nerves, Thus, inhibiting uptake results in tre Accumilation of the neurotransmitter in the synastic cleft This is { the mechanism of action of tricyclic antidepressants, since they inhibit - noradrenaline uptake.Depression is associated with decreased noradrenalinelevels, For these studies, the Same Protocol which was previously used to determine the effects of el ic fields on noradrenaline uptake Was used(22). This involved exposing confluent monolayer culture of Pc12 cneray  aeeiolabelled noradrenaline in the presence or absence oF erste conte fee L230 minute exposure period, the cells were washed and experimental dishes received the same electromagnetic field exposure, but Table 1 EFFECT OF SCALAR ENERGY ON NORADRENALINE UPTAKE (Cpn/my protein) (cpn/mg protein) 10min 30min 10min 30min 1. 1111 = 4065 1079 3500 2. 880 7032 1137 5009 3. 1076 4520 1110 - 3592. 4. 9033 6035 ~ Ss. 4277 4041 6. 4117 - Mean 1022 §507 1109 4435 S.E.M. +72 4841 +17 +480 effect on nerve cells. The inhibition of uptake is time dependent with no. significant effect observed after a 10 mimite exposure. It is  .. -4- Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9
ed For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 unknown whether exposure times greater than 30 minutes would produce a arger effect or whether the effect would be maintained either .in the continued presence of the scalar field or not. These questions will be in additional experiments. Since inhibition of uptake occured after only 30 minutes, it is likely that this effect is an early biological response to scalar energy and may be the trigger for subsequent physiological changes that result in improvement of a wide variety of clinical diseases. Since the scalar energy inhibited the uptake process, it is acting like a traditional antidepressant drug, thereby explaining the antidepressant action of the Tesla watch experienced by some individuals. . These results also indicate that scalar energy, like electromagnetic fy energy, can have a direct effect on the cell membrane. Since both scalar > ard electromagnetic energy have the same effect on neurotransmitter | uptake, the results offer indirect support for the crystalline Transduction theory. Thus it is possible that the scalar energy could be ‘converted to electromagnetic energy in the membrane ard the latter actually causes the biological effect. However it is also possible that the scalar energy will have a direct effect on the uptake system. Needless _ studying the biological effects of scalar energy and for ultimately characterizing its mechanism of action. : 1)" Nonlinear Electrodynamics in Biological Systems", Adey,W.R., ‘:. Lawrence, A.F. (eds.),Plermmm Press, N.Y. (1984). 2) Loosley-Millman,M.E.et al. Biophysics J. 40 221 (1982) 3) Basar,E. American J.Physiol. 245 R510 (1983) 4) Barllow,C.Y.et al. Nature 332 312 (1988). ' 5) Lawrence,A.F. ,Adey,W.R. Neurolog.Res. 4 115 (1982). 6) Bednar,J.Internat.J.Radiation.Biol. 48 147 (1985). 7) Tesla,N."Colorado Springs Notes" Nolit,Belgrade,Terazije 27, Yugoslavia. : 8) Aids: Biological Warfare, Bearden,T.E.,Tesla Book Co. ,Texas (1988) 9) Bass,R. Proc.Tesla Symposium,p89,Internat.Tesla Soc. ,Colorado Springs (1984) . 10) Rauscher,E.A.,Technic Res.Lab Report PSRL-3107A,San Leoardro, CA. 11) Quantum Mechanics and Nonlinear Waves, Burt,P.B. ,Harwood Acad.N.Y. (1981) 12)Bearden,T.E. PACE Newletter 3(4)p10(1982). , 13)Byrd,E.A. 35th ACEMB Conf.Abstracts,p218,Sept. (1982) . i )Rein,G. in "Mechanisms of Psychic Perception" ,Millay,d. ,Sirag,S-P. (eds) press. 15)Byrdl,E.A. in ELF Cocoon Internat.Catalogue,St. Francisville, Il. (1987). 16) "Psychoneuroimmmology", Ader,R. (ed.) ,Academic Press,N.Y. (1981). . 5- Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9
_ Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9 17)see Byrd,E.A. Archaeus Journal J] (1)pl (1983). 18)Puharich,A. 14th Ann.USPA Conf.on Bioenergy, Dayton,Qhio (1988). 19)Greene, L.A. ,Rein,G. Nature 268 349 (1977). 20)Greene, L.A. ,Rein,G. Brain Research 138 521 (1977). 21)Dixey,R. ,Rein,G. Nature 296 253 (1982). 22)Rein,G.,et al.Proc.9th Ann. Bicelectromag.Soc. ,Oregon (1987). -6- Approved For Release 2001/03/07 : CIA-RDP96-00792R000500240008-9