+ : Approved For Release 2001/03/07 : CIA-RDP96-00792R000300430003-5 At a low rate of O, consumption, the output of CO, decreased even more. This suggests inhibited glucose oxidation, enhanced gluconeogenesis and increased fat metabolism. 12. Ketone Bodies The 23 qualitative tests for ketone bodies in the urine showed strong positive (4 times), medium positive (4 times), and weak positive (15 times). Also, 6 quantitative tests for ketone bodies in the urine were above the normal (50mg or less). Maximum daily output of ketone bodies was | g. Repeated positive outcomes of urine ketone bodies testing were due to increased utilization of fat as an energy source. In extreme hunger, blood ketones are considered a major energy source for some organs when glycogen is used up and glucose input is limited. This could be considered positive, however, not all the tissues can utilize ketones as an energy source and their increase can disrupt homeostasis. From the above tests, one can not conclude that the increase of ketone bodies was a pathological phenomenon. Conclusion These points are drawn from the study of this case: 1) Qigong fast (Bigu) exists, but not as a complete fast. 2) The young girl maintained essential life activity, physiological homeostasis, normal life style and low physical activity under severe energy and nutrient deprivation during this 30-day period. However, decreased body weight, low energy levels, repeated positive urine ketone bodies and inhibited digestive function were also observed. 3) Qigong fast over a period of time could cause lowered metabolism and oxygen consumption rates in resting condition, 120 f | lowered concentration of T3 in the blood, decreased respiratory index, significantly lowered energy and nutrient intakes, little or no stool, lowered level of nutrient elements in urine, decreased secretion of saliva and decreased enzymatic activity of saliva diastases, lower range of stomach contractions and slowed digestive response, decreased body weight, and increased ketone bodies. In summary, the above ratings can be considered a method for preliminary evaluation of Qigong fasting. Of course, the measure can be improved. It is significant that essential body energy and normal life activities were maintained for at least one month of Qigong fasting. Influenced by Qigong, various approaches might be involved to utilize energy efficiently, to save energy, and to maintain normal body functions and low activity levels under conditions of severe calorie and nutrition deprivation. This information could be very important for disaster and military medicine. We suggest, however, that future study of Qigong fasting be under the guidance of modem science and technology, with careful research and data acquisition, before any conclusion can be made. While it is too early to draw a final conclusion regarding Qigong fasting (Bigu), the observation of some abnormal symptoms in this case indicate that a long term of calorie and nutrition deprivation might have a harmful effect on young people or developing children. Therefore, we would hope that this fast not be promoted among the young and that, among adults, this fast be carried out under medical surveillance. * Published in Chinese Qigong 1990.4, PRC. 121 Approved For Release 2001/03/07 : CIA-RDP96-00792R000300430003-5
Approved For Release 2001/03/07 : CIA-RDP96-00792R000300430003-5 8) The Study of Qigong Effect on Bacteria Strain Improvement Yan Xin (Chongqing Chinese Medicine Institute) Li, ShengPing; Sun, MengYin; Dai, Zhongying; Zhang, PeiLin; Meng, Guirong; Liu, ZhiYong; Ma, GuoLian; Zhu, Qunying (Tsinhua University) The scientific research of "Qigong" has shown that the “human body can change property of matter by changing its molecular structure without physical contact". Based on previous studies, this study proposed the idea of using Qigong to change the genetic property of microorganisms so that bacteria strain for industrial purpose can be improved. The fundamental feasibility research consisted of the following three aspects: 1) Observation of the effect of Qigong treatment on various biological macromolecule, especially on DNA, which carried the genetic information The experiment showed Qigong has effects on amino acid, protein, RNA, and DNA. The Qigong treatment on fish sperm DNA caused the intensity of the UV 257nm absorption peak to change over 50%, and this change could be hypochromic or hyperchromic, that is, the absorption peak shifted to either side along 257nm. 2) Qigong treatment on bacteria Experiment data showed that bacteria after treatment not only changed bacteria survival rate but also accelerated the growth rate. 3) Qigong treatment on industrial bacteria strain 122 Experiments showed that treatment enhanced the bacteria growth and increased the production of fermentation. The main features were: (A) The bacteria strain showed very low tumover rate after treatment, obviously different from the other classical chemical, physical treatment methods. (B) The strains became highly specific, and the distribution spectrum was wider, thus, the time required to select high-yield strain was shortened. (C) The bacteria colony morphology was very normal after treatment, this is different from the results of other treatments. (4) This treatment did not pose health hazards to the operator, and is environmentally safe. (5) The bacteria growth rate was enhanced, fermentation cycle was faster and production increased. (6) The strain resistant to treatment in classical way showed obvious improvement after the Qigong treatment. (7) Genetic study showed the new strain from Qigong treatment was very stable, with high yield. The systematic study demonstrated that Qigong treatment can become a novel biotechnology. The high-yield strain produced by this method showed promising potential for industry. 123 Approved For Release 2001/03/07 : CIA-RDP96-00792R000300430003-5
? o¢ Oo Oo © CP) T+ Oo © o¢ Oo Oo oO ow N o>) NM Oo id co o oa a Oo < O DM (=) : ==) ee a ; Oo rN , O r oY) r © @ x ® ax FS ‘me LL r°] ® 2 : O ; P< 9) Qigong Treatment on Yeast By Hydrocarbon Metabolism Group (Institute of Microbiology, Academe Sinica) We have conducted a mutation study on wide type Candida tropicalis in an attempt to get the mutation stain capable of producing long chain, low oxidatation hydrocarbon from crude petroleum by using chemical mutagen such as guanidium nitrate and nitrite salt, UV irradiation, and laser. We recently collaborated with Qigong master, Dr. Yan Xin, to do mutation and to select the mutant strain by Qigong treatment. After a two- month study, we got positive results. The wide type and mutant have no morphological difference after the Qigong treatment, but have a significant difference in growth rates. The wide type produced uniform colonies; the mutant produced colonies in different sizes. The metabolism study on two strains of mutants showed that, for #3, strain 40 out of 140, (28.6%); for #5, 34 out of 128 _ (27.9%) have the positive change (hydrocarbon yield increased above 15%) and 28 out of 140 (#3) or 20%, 50 out of 122 (#5) or 41% have the negative change (hydrocarbon yield decreased over 15%), and 72 out of 140 (#3) or 51.4%, 38 out of 122(#5) or 31.1% have no change in yield. Further we selected 10 high yield (4% increase) colonies from #3 strain; then repeated the selection. Two colonies were reproducible and have average increased yields of 44.5% and 33.5%. Also, for #5, we found 2 colonies that were high yield in production, 34.1% and 21.3%, respectively. We are going to further study the stability of those high yield mutant strains and try to expand the production industrial level. 124 10) The Observation of Antibiotic-Producing Bacteria Strain Processes and Selected by Qigong in Industry Level By Northern China Pharmaceutical Company We collaborated with Qigong master, Dr. Yan Xin, and a group from Tsinhua University to explore the potential of Qigong treatment to improve antibiotic production bacteria strains. After strict selection procedures, we have obtained some good quality strains and now these strains are being tested in large scale. The initial results showed that Qigong method is effective, simple, efficient and harmless to human health compared to other presently used chemical and physical mutation methods.The miracles in this method are waiting for us to explore. 1. Selection of mutant strains of Streptomyces grisells induced by Qigong On September 19, 1989, we treated S. griseus strains 8512 and 8801 with Qigong, and did three parallel experiments. The test group spore survival rate is 62%-86%; colony morphology had no significant change. The 8512 strain initial selection was 53 colonies, a positive change of 71% and a 23% increase over standard procedure. The 8801 strain initial selection was 53 colonies, a positive change of 20%. The spore growth rate after treatment was faster than the control group. Also, sugar and nitrogen metabolism were faster than for the control group. 2. Qigong treatment on Streptomyces rimosus 125 Approved For Release 2001/03/07 : CIA-RDP96-00792R000300430003-5
On December 19, 1989, we treated the terramycin producing bacteria Streptomyces rimosus with Qigong. The spore survival rate for the test group is 50.3% to 76.7%. Initial sélection was 145 colonies, a positive change of 100%, and a 13.5% increase over standard procedure. 16 colonies were selected from the initial selected test group, and 46 colonies from initial control group, for further study. After 3 repeats, the test group showed a 100% increase in yield compared to the control group, a 14.2% increase over standard procedure. The test group grew faster than the control group. 3. Qigong treatment on aureomycin-producing bacteria We did a mutation study on these bacteria by Qigong treatment on Dec. 19, 1989. Among the four parallel experiments, all the test group showed significant difference with respect to survival rate, and growth rate. Spore survival rate was 100%. Initial selection was 75 colonies, a positive change 62.3% over standard procedure. 5 colonies were selected for further test. One turned out to be the best strain this year; yield is 18% higher than control group. 4. Qigong treatment for Penicillium We did the treatment on Sept. 19, 1989. The spore survival rate was 100%. Colony morphology was similar to the control group. Initial selection was 67 colonies, a positive change of 34.3%, and a 17.14% increase compared with the control group. 7 colonies were selected for further study. 3 repeats showed the test group was 10% better than the working strain used for current production. * Published in Chinese Qigong 1990.4, PRC. Chapter 4 Eyewitness -5 db03 1) Dr. Yan Xin’s Lecture Tour Across Ameri Vicente N.Ongtenco President, Canada Qigong Research society Counsel Member, World Medical Qigong Association 92R00030043 June 19,1990, Dr. Xin Yan, a Chinese physician who i believed to be the most famous and the most powerful knowl? qigong master and psychic healer, arrived in San Francisco ten attend the International Qigong Congress as one of its principafy speakers. Traveling with him were many other Qigong masters{z physicians and scholars, among them Dr. Zuying Lu, a nucleart physicist, formerly professor of Tsinghua University presently professor of China High Energy Physics Research Center, Dr Ly_ is Dr. Yan’s closest associate in scientific research. <4 The two have worked together for several years in Beijing om research to find out the scientific basis of the Qigong powers such as distant diagnosis, distant healing and instant healing. © Because of the miraculous qigong power Dr. Yan possessedg, and the efforts of Dr.Lu and other university researchers, thi? little known ancient art of human development has now beer brought within the realm of science. w Researchers in Tsinghua University of Beijing confirmed ir§ laboratory experiments that Qigong did cause changes in matte#L not only in the molecular structure, particularly in DNA and RN moleculars. Internationally respected scientists in China have endorsed these findings and encouraged further studies. 5. Though scientists are generally careful in releasing reports om such matters, astonishing abstracts have been published in the proceedings of the First World Medical Qigong Conference held 6 127