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P waves .However, the effects of (1) and
(2) were observed clearly in
all receivers. During
hypnosis led by means of non-verbal method, the
(3 waves of the subjects still appeared on thb
linguistic area. These results indicate that hypnosis
is the verbal suggestion at all, while qi is
not a so-called placebo, but something that can be transmitted transpersonally.
THE EFFECT OF QIGONG ON EXPERIMENTAL
IN-
FLAMMATION
AND
NEUTROPHIL
CHEMILUMI-
NESCENCE
Yan Yizhao,Chen Xiangyin
Institute of Basic Medical Sciences
, Chinese Academy of Medical Sciences
,Beii
jing, 100005
;
In order to evaluate the possible role of qigong in treatment of some inflammatory dis-
ease^, the
effect of qigong on the experimental inflammation (paw edema) and neutrophil
(PMNs) chemiluminescence (CL) were studied.
Wistar rats
, weighing 200 — 250g were used in this investigation. The paw edema was
produced by an injection of 0. 1ml of a
1 % suspension of carrageenin in 0. 9 % saline into thej
hind paw. The length round the paw was determined six hours
later. PMNs were isolated
front the peritoneal cavity of glycogen treated rat. The CL system consists of 5X10
6PMNs,;
0. 1+1 of
0. ImM luminal and Img of opsonized zymosan
. The CL reading was determined byj
a Biological Chemiluminescence Instrument.
The results showed that (1) Qigong could inhibit carrageenin-induced paw edema. The
increased length round the paw (ILRp) in the qigong group was
0. 557 + 0. 035cm (n = 26,
P
<0. 01), and was similar to the hydrocortisone group
(0. 206 + 0. 028,n = 26)
(2) Qigong
could stimulate PMNs CL in vitro. The CL reading in the qigong group increased from 168.
7 + 16. 2 (n=22)to 236. 3+ 23. 5 (n= 22,P<0. 01).
jNeutrophil (PMNs)
is an important inflammatory
cell. PMNs activation and products
(lyspsome enzymes and oxygen free radical) may play an important role in the pathogenesis;
of acute inflammatory reaction. In the present study, we have found that qigong can prevent
the development of carrageenin-induced paw edema and stimulate PMNs activation in vitro,
j
The possible mechanism of the qigong effect
is very complex and will be studied in future.
!
1—38
PROTECTIVE EFFECT OF THE EMITTED QI ON THE
PRIMARY CULTURE OF NEUROCYTES IN VITRO A
GAINST FREE RADICAL DAMAGE
Tang Yipeng,Sun Chenglin ,Hong Qingtao,Liu Chunmei, ,Li Xiaoming
Institute of Qigong Science , Beijing College of T. C. M. Beijing 100029, China
This paper reported the protective effects of the emitted qi on cultured neurons damaged;
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by free radical
. The neuronal cell' s suspension was made with the cerebral cortex of Wistar
O-day newborn rats and
it was cultured in dishes. At 8th~day postplating , the dishes were
divided into
3 groups, i.e. the emitted qi group
, damaged group and normal control group.
The neurons of the
first two groups were injured by hydroxyl free radical generated m the
ascorbic acid system. We observed that the brightness of the somatic margin in the damaged
group turned from bright to dark, the volume of neuronal somata increased
, the number of
neissl boby decreased
, the effusion of LDH increased and GSH-Px activity changed obvious-
ly. These evidences indicated the neurons in the damaged group had swelling or degenerat-
ing. But
in the emitted
qi group
, the effusion of LDH was
less than that of the damaged
group. Both groups showed an obvious
difference (P<0.
01-0. 001), GSH-Px
activity
changed a little. The results show that the emitted qi may act as a scavenger of hydroxy radi-
cal and protect the nerve cell membrane.
I
THE EFFECT OF THE HUMAN ENERGY FIELD UNDER
THE QIGONG STATE ON THE STRUCTURE AND FUNC-
TION OF RED BLOOD CELLS IN EXPERIMENTAL RATS
WITH BLOOD STASIS
Liu Chunmei,Sun Chengling
Beijing College of Traditional Chinese Medicine
, Beijing 100029, China
Qigong is newly recognized by people as a modern medical means to be used for prolong-
ing
life and enhancing the human immune function. Especially
, it has a particular action for
treating chronic , difficult and complicated cases. The action of drugs on organic microcircu-
lation was reported before in medical literature , but the effect of the human energy field un-
der the qigong state on the structure and function of the erythrocyte membrane has not been
reported yet.
Through the investigations of the human energy
field phenomenon, Inyushm
put forward an energy stroma model about the human energy field according to many books
written by researchers. This stroma model constitutes the stroma structure which can make
organisms exist. Some methods developed from this energy stroma model are now used in
medical treatments. The experiments made by our office before have proved that the human
energy field under the qigong state can improve blood rheological property of rats with blood
stasis.
Researching results show that the human energy field under the qigong state has the
action of protection of red blood cell structure and function in animals with blood stasis, and
that the content of red blood cells and peroxide lipid of liver tissue can be reduced
, sialic acid
content of the erythrocyte membrane can be recovered. Thus, it follows that the human ener-
gy field under the qigong state can relieve or prevent organism cells from free radical damage
, and that the human energy field released by qigong doctors can, to some extent, regulate dis-
ordered organism. But the protection of membrane protein structure damage
is not good. We
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havej used animal models with blood stasis to observe the change of symptom due to blood
stasi^and then to observe the action of the human energy field under the qigong state on red!
blood cell membrane, and finally to research into the mechanism of the human energy
field;
regulating organic metabolism.
1—40
ANALYSIS OF THE EFFECT OF THE EMITTED QI ON
HUMAN HEPATOCARCINOMA CELL
(BEL-7402) BY
USING FLOW CYTOMETRY
Chen Yuanfeng
Shanghai Institute of Traditional Chinese Medical Science .Shanghai 200030, China
^ased on our previous studies of the cellular effect of the emitted qi on human hepatocar-
Cm°T
Cel1 (BEL-7402)
in this experiment, the state-of-the-art technique of flow cytometry
(FCM) was employed to analyse the influence of it on BEL-7402 from the angles of cell divi-
sion 9 kinetics and genetics.
Repeated reults indicated that the emitted qi treatment inhibited the higher rate of DNA
synthesis
in BEL-7402 and decreased the concentration of DNA
. Meanwhile ,the cell cycle of
BKL 7402 was correspondingly altered. G0,G1,G2 phases became more obvious by repeated
treatrjients ,but S phase appeared to be smaller.
After the emitted qi treatment, chromosome
karyotype changed too
. These results demonstrated that the BEL-7402 cell could be restored
to the. normal to some extent by qigong action. For the further confirmation .naked mice was
inoculated with BEL-7402 and the treated cell respectively
, and
it was surprisingly observed
that the tumour was only induced
in the naked mice inoculated with BEL-7402,
whereas
nakedj mice with qigong treated BEL-7402 grew well.
This result was further evidenced by
the FGM analysis of cells isolated from in situ tissue.
l_
THE effect of the emitted qi on agglutinat-
ing REACTION OF HUMAN PULMONARY ADENOCAR-
CINOMA CELL (SPC-Al) MEDIATED BY CONA
Chen Yuanfeng
Shanghai Institute of Traditional Chinese Medical Science .Shanghai 200030, Chi-
na
Intercellular recognition and contact inhibition are the main characteristics of the cultured an-
imal cell .which usually appear as agglutinating reaction mediated by some plant lectins, such
as ConA.
It has been proved that there
is close relationship between the agglutination and
glycoproteins on cell membranes. The sort and distribution of glycoprotein as well as
its in-
teraction with relative receptors can influence the agglutinating reaction.
In general .normal
cells expresses less degree of agglutination than tumour cells.
Therefore, in this experiment,
we used SPC-Al cells as the material, and the effect of the emitted qi on cell recognition me-
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diated by ConA was studied* Mechanisms involved in the cell recognition
, such as cell mem-
brane-bound glycoprotein and receptors were also investigated.
It was found that the emitted qi treatment reduced the extent of agglutination
, and this
effect became more obvious with increasing times of treatment. Meanwhile
, the forms of gly-
coprotein on cell membranes were also changed
, displayed on a SDS-PAGE
, developed by the
specific Schiff's staining method
, among which some bands disappeared and others emerged.
This variation may play some role in the process of cell recognition. When the FITC labeled
ConA was used
as
a fluorescent probe to detect ConA
“ receptor” change
in
situ with the
FCM technique, it was further observed that the amount of ConA “receptor” decreased and
its distribution was also changed
. These results may partially account for the reversion effect
of qigong on SPC-Al
cell.
1—42
INHIBITION OF HUMAN NASOPHARYNGEAL CARCI-
NOMA CELLS IN VITRO BY THE EMITTED QI AND Y-
RAY
Cao Qiyuan
ci) ,Li Yongqiong
CI)
, Cheng Chenqing
(2)
, Liang Jianxiong
(2)
(1) Cancer
Institute , Sun Yat-Sen University
of Medical
Sciences
, Guangzhou
510060
( 2
)
Dept.of Radiotherapy
, Tumor Hospital
, Guangzhou 510060
Using human nasopharyngeal carcinoma
cell
line (CNE-2) as the target
cell, the cells
were planted into 96 well plastic microcultured plates, 50 cells per well, one plate as a group.
There were 4 groups
: the control (c)
, irradiated by 2Gy 7-ray (R)
, treated by the emitted
qi
(Q) ,and treated by both of them (R+ Q). After two days culture, the cell clone(^8 cells)
under an inverted microscope was observed and counted. The mean value of cell clone (X+ S.
D.
) in the R+Q group was
9. 2 + 2. 5, markedly lower than the R group (15. 8+ 2. 4). There
was a marked statistical difference (P<0. 001). The kinetic study showed that the Q group
at 48 h. the level of mean value of cell clone (16. 5 + 2. 2) approached to the R group. After-
wards
it went up again apparently. However
, the R group continuously decreased through 48-
96 h. The emitted qi alone was able to kill or damage the CNE-2 cell in vitro, but this action
seemed to be higher reversible than arising by irradiation of 7-ray.
1—43
A PRELIMINARY STUDY OF THE EFFECT OF THE
EMITTED QI ON EXPERIMENTAL ANIMALS INFECT-
ED BY PNEUMOCYSTIS CARINII
Zhang Jinmei, Zhang Ciling ,Chen Fakai
Sun Yat-Sen University of Medical Sciences
, Guangzhou 510060
Pneumocystis carinii (P. Carinii) has emerged as the leading opportunistic pathogen and
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