cia Eileen Sok ERS 2: A Se er aoe .'ibooe Ese WORD ABOUT BIOPLASMA -7 _—-«—anr—eeeee ' mh ' . 4 A t gee _ ——jnteraction of particles comprising it. During the disturbance o a _———_pioplasma structurea certain amount of-heat should be discharged. —in __—eonnection-with-this;—one—should-not—failteo-mention—the—experiments-— - arried—out—by—the_-English_discaverer Hill, _in 1928. Hilji discovered_ that the amount of warmth given out by some organic molecules during the absence of oxygen over a period of 24 hours is very great and. ——cannot solely be explained as béing the résult 6 yeolysis and the — _——_ fragmentation of ATF, etc. (ATP ?--~Eds)~-According to-Hiti; for-l— __—_—-gram-of-muscle—{ sinew)—one—obtains—5—gms/cal.—-This—exceeds—by—5—times hich-biochemical processes given out during muscle death me_kind of unstable structure is disturbed, depreciating its - —~energy in the form of heat. We assume chak such a large discharge of ——fhReat is a result of the df{strubance of the bioplasma. This is the _ principal acculator of energy in the organism. But; hundreds of———— —_—— eriments—are—stili neccessary—to—finaliy—prove—that—the—-observed—_—_ ee *thermal—discharge"_accompanied_by the radiational discharge_is, above all, connected with the loss (extinction) of bioplasma. Further a discoveries about the bioplasma will help to uncover many of its prop- ‘"——“Srties. This is why we devote to this field of discovery so much time. -———_——_Extraordinarily interesting are experiments carried out—by_us-———— --— which -have-enabled- —us-tco-prove—a-chain-of—well—known--properties—of— the bioplasma. eee It_all_ began with the attempt to give an explanation to experiment made by A.G. Gurwich, carried out in the ‘twenties. Two roots of “ai fferent onion plants were forced together. The distance béetweén ~--them being from 2-3 mm. —The irradiating root—tinductor)—united with— — the-onton-tike plant-were—reinforced—in—a-stative—(mechanical- support) _—and—its—end_was_trained_on_the zone of intesive cell fragmentation ____measured_by_ the other onion-like plant placed-horizontally. After this the number of cell fragmentations on the irradiated and non-irradiated sides of the root were counted. “~The experiments showed that from the tip of the root _of-the————— ~—inductor; radiation—in-the-form-of a-narrow-trained-beam-was—emitted,— -—-which—-stimulated—cell—division—on_one_side_of the root._Barriers .made of quartz glass did not _disturh the_effect, but_ordinary_glass absorbed _the radiations. This spoke of the ultraviolet nature of the radiation. a 7 In our laboratory, we decided to check on the experiment, but — ‘instead. ~of using the small root applying the radifation; we-placed—a ~ spectat-plate covered -with—photoemutston,—a-new-acceptor-for—photo ~emulsion—had—been—developed.—__The—photoemulsion_was_protected_ from __. che_samll_ root by light filters_allowing_ultraviolet to pass. ssi. ee (Caption of illustration "8: Example of noncontact registration of mitogenetic radiation of small root of onion.) After long séarchés, finally an optimal process of expertimenting— ““was~found. fhe photoemutston showed-the—-image of the-object—in-—the-—rays ~ of-intrinsiec-radiation—(see—tllustration-Nr-8).—________- --_—__Analysis has shown a small angular divergence of radiation , --that_is, it is possible to suggest that every point source on the objec _in_fact generated laser coherent radiation. This once more proved, tha’ le nature of radiation is plasma in character. And the radiation —— tselfts~enyaged with nonequilibrium distributions of etectrons—in 7 iestructures;—at—the—same—time it —ts-neecessary_te—mention—that—ther: _~48-a_larger_amount_of excited_electrons_in biostructures_than those _ --“nich_do net possess excitability. : : — The photograph shows a living small root which is conditioned hy _..£Focesses .taking piace in the bioplasma. The dying o @ SHall root; wd “r-gs about light radtation which itiuminates- the-photographic—film—
™y RR ms nd EEE SSS mea ng oe —! (eo ee — ae wee a _ oe __ Approved For Release-2000/08/07 a3 CIA-RDP96 -00787R000500080004-0 —____ a ooo rr a oe a ee a a a we eee eee eee eee ee —_— : weer Fee.ccude aaa Frelint z __—A-WO RD—ABOUT—BIOPLASMA It_was observed that a sharp_increase in electrobioluminescece_wa observed at 2 AM and 6 AM. Hence, a 24 hour dynamic property of electrobloluminesence has been _: discovered. By what is it conditio ——ear (Text of illustration lor 24 hour dynamics of the intensity of __-_etectrobtotuminescence—in-the-skin-of-the-ear—of—a-rabbit<)}-——-—-_—_— _____———Specially—organized—experiments—speak—of—the—dynamics—of—Luminese as_reflecting the 24 hour progression of changes in the intensity of — ——~the electrical field between two gigantic plates of a condenser--- meaning the ionosphere and the earth. The ionosphere carries ac -———_gonsiderablé charge, the earth being negatively charged. The tcal change of intensity by night ts condittoned by the tighter— ____ emission—of-charged_particles—from-the—surface—of_the—rabbit's—ear—— during_research_made_into luminesence_in _high-voltage_impulses_accordi: ~___to the method used by Kirlain and'assistents. We have begun to learn that this phenomenon is more profound suggesting that the structure of the biofield should ~ also rhythmically fluctuate. Research Has been -——_ made _into-the-24-hour—changes—of—antsotropy-of-the-biological objects. _-__The—ability—to—turn—the—p1 ane—of-polarization—-of the—ray—has—turned——_ ____out._to be dissimilar during night and day. What is characteristic is _ that _a change in the direction of the turning of the plane of polari- “zation takes place at different hours of the 24-hour day. —~. And sé, the ordered structures of the biopfasma isn't so "crude". --——The-structure-ts—changeable; and depends upon_the-changes of the ————_ ———rhythmically—pulsing-structures-of-the—-gravitational—-and—-electrical—_-~ .._.field.__Experiments._have revealed _a completely new field----disoaverin¢« ___the science of biorhythms in connection with the conception of the bio- lasma. Ce, . Pose rhe biop lass IS Saturated WIE “vibrations possessing very ‘~—sphecival characteristics; such as: —coherence;—and_potartaatton;———_ ~-—Vibratioens—in-the—red—band-eof-the—spectrum being—dominant.—The—red——_ ----resonance_is_in_itself a symbol of highly=-organized life. That, is in ..._fact_why it is possible to have such fine (precise): regulation_of biological processes, with the aid of radiation energy generated by the " ““helium=neon Lasers. OO “~~~ As far Backas the 30's, E. Bauer said that pot&rized ray emtssion ~ should_-possess—Larger—biolegical—activities—than—depolarized—light.—— ~ However,—experimental_.data_on_the-role_of_polarization—in_photobiologic - reactions is_ small, although the formulation of such_a_problem_in_our__ _Gays is all the more topical, in connection with the neccessity of _carrying out research into the mechanisms of the activity of monochrom- atic polarized coherent laser radiation. " in~our—laboratory—we—carried-out—a-—cycte _of-work—on—discovering— the-Fole_of_polarization_and_coherence_in_the_biological—activity——— --Of_light. It was learned, above all, about. the influence of polarized ~ ong_nonpolarized light on breathing and photosynthesis during equivalen __ intensity of light. The breathing of the shbots of plants was déterm- __ined by means of a gas analyser —_ “analyzing CO>. The Héelium-Neon ase with wave h—-6328-A-with-ray_intensity—fal Hifig-on the camera at—o-i-nW/—em"_was—used—as_the-source_of-radiation, The weak@ning-of-— ~ 5\6—raysS was achieved with the help of neutral light filters and the _ . Sepolarization of radiation was achieved with the aid of ground glass. ___Depolarized radiation brings about a sharp decrease of respiratory * tensity. The experiments were well carried out. Thus, it is PCSSibte—to-spaz _ ak of the large role of linear polarization, in the mh ee . p .. Fuetocatylic activity of 11 . -€SDtration 5eR ght on the complex chain of processes during a ll a
———— re fee eee - eee eee os wee —Approved For Release 2000/08/07.: CIA-RDP96-00787R0005000 BQ001-0. 5 - py --, | TT=SF 7 TET“ cis R WORD ABOUT BIOPLASMA -~1o __—_—_ Possibly, the effect 1s condttetonea bi y the electric and optical ____pelarity—of—the-—bio substruc ture;—about-which-we—shatl-speak—belowy— other_experiment,—we—_made—research—in to—the—influence— . of polarized light on_the s ynthesis of pigments a—For this, twa groups — of plants were grown. One under polaroid film, another under a . ——-——peutral grey Tight filter. TIilumination in both cases constituted _————— from 1000-1, 2000 -LK The -res alts have béén preséntéd in table 3. _____—_{Gaption-of -table—3:—the—content—of—pigments—in—teave s,_ 4-day —_—___ —— Te phenomenon of accelerated synthesis of pigments during the — - activity of polarized light is observed. wt follows that.the photo- -————~gynthetic productiveness of growth will also be higher. The experi- | .. ——ment~shows that” changing the regimen of polarization of light we —_——ean-directionally—increase—the-pheto-synthetic-productivity>—one-of- __._the most convenient—group_indicators—of -photosynthetic-effeetiveness— : is the rapidity of oxygen discharge. For the accurate measurements _ of this magnitude, it is possible to use the polarographic method of _ ~ —~~@éetermining oxygen which contains in itself a high degree of sensi- - ———tivity; non=tinertta—and-allowing-the obséi rvation of photosynthetic — --_—-change—in—various—experimental—condi-ttions-———__—— — _—___—_—__As_is_known,—the_polarographic- curve—of_oxygen—appearance-has — two_sharp plateaus parallel to the axes of the potentials. The “~~ plateau manifests itself as a result of the fact that. during the “potential corresponding +s Lts beginning, the rapidity of appearance ~-——reaches signtftcance—at which alt the-motetulés of thie substance,in - ——this—ease,—oxygen,—rapidly—form—treform,restore}.—Phe-establishment- -_. of oxygen isa very comp lex_process,_going_through_a-whole_series__.__ of more simple elementary stages: — The flow of the first half-wave, or semi wave, is conditioned by _ the restoration of oxygen to the point of peroxit ity with the binding TG ointng)of—two-electrons.— Depending—upon-the-tn tensity of the ; -— Processes—going—on,—the—-magnitude—of—the—restoration—of—the-current— Changes, about which it is pas sible to speak_about_a—concentration—of -__restorative bodies, particularly of oxygen. = To express the results in absolute units, a pertodical grading _ _ Of the cell according to the oxygen content was Carried out. The rat Oof- distributing-the-appara tus-wasdet_ermined according to the -Magnitude—o f—the--limtting—current,— accompant ed-by the-concentration— cL oxy gen_at_2]1 %, and comprised 9 x lo=¥ moles/ 1— Apart—from-that.,.— =¢fore each measurement, the volt-ampere curve of. pure_water, or buffer “aS checked, where the object experimented upon was Suspended. The concentratign of oxygen in the water within the radius of the cell was = SO25~xX-t —motTes/L.—_In the ser fesof expertiménts made to learm -- Svout the -influence—of polarized—and—depolarized—taser—tight;-kinette . - Cirves_ of oxygen restoration_we re—obtained,—accordingto-whichit——_ . -+$_Possible to speak of the change in the rate of photosynthesia oe he experiments were carried out according to the following nema: — - “t}~re cording—of -the“intttat-state—o fchioreprast suspension —.- “+2 +—activating-the—laser— and-simultaneous—recording—efthe—eurve-of — Oxy gen.restoratt’ . ~#)___Laser il lumination in the period of a definite time period (la=30 - ii__the depolarization of radiation... As is the rule, in initial cgnditions the concentration of oxygen _.. (SiStittted trom o.%6 -o.78 x 16 moles/L . With the activation of _, @t@ las€¥, a Gecrease OF initial level t6 6.675 x Io 3 moles /L has - A -22n~obse eigue 0 | DESSLOUTETROC0SB00S000T-Brotostim- Oo USIB DEPARTMENTS ONLY a be “td
— zt i Ei Fa : —_ _——Approved For Release 2000/08/07 : CIA-RDP96-00787R000500080001-0 ——a ___-——-—_ Ak WORD ABOUT BIOPLASMA =11 Tg, ina pert = wl me ee eee 7 s,—in_a_period_oflo-miy utes—,;—the-—concentration of-oxygen Incréas: and_ constitutes 0.77 x 310—_moles/-L—.—_And-after-the-elapse-of-—- 30 minutes, 1-65 x lo " moles /L_. If the suspension_is—illuminated ———_ with depolarized laser light, ght, then the curves sharply decrease, some— _—_—— times below the initial level of 0.155 xlo moles /L., ___—Changing-the-scheme—of—the-experiméen t--=-Illumination by depdlarized _____ and then-polari zed—-lLaser—light-brings—about-a-simftlar re action. _ We give here the mean_data_of_the—experiments—carried-out——— ———~th moles/ liter: --———_fnital level of oxygen concentration—--0.75 xlo-3 ng ee} depolarized laser light ------------- —-o.7o x lo iitumination—-of-suspended-objece : _____by depolarized—laser—light-—-ove r——________- polarized laser light -------------=---9.775 x lo73___ ; _______In_another_series_of-experiments-the—task-was—to-exp lore the —— _ nergy output of photosynthesis where use was _made—of—He-Ne-lLasers— -——$2 cohe rent pol arized beams. For this purpose, _a_photocalorimetric_. méthod of a gradient-type was app lied, whose advantage was its great “sensitivity (6.002 dégrees C) and which is extreme ly important. in __-—__biolegical—research,—The-temperatureof~ the -6b ject being able to insignificantly vary—from—the- ~ ‘temperature-of~the-surroun ding medium. The working principle or the_calorimeter—i s—contained-in-—- ~“""~temperature changes of the s uspension of chloroplasts during-laser — ~~~radiation and in remeasurement of temperature of the same radiate d__ ~-7-—~suspension, but in -non=phote=synthesized conditions. . The differences : in—temperature—of—non—-photosynthesising (large T nps ) and pho tosynthes- ..__ising_T_suspensions_are_proportienal—to—the—ambBst of-energy_ _Stored uring photosynthesis, The energy outputk s— E-=—1 Tv Paps -——--—_The—amount—oef—absorbed-energy—of tase rgradiation was measured — AL th..the help_of _IMO-2_and-comprised:-8—-x- lo “-ergs/secew-~"Durin g-the ~~ radiation of the chlo, roplast_suspension hy laser, -thé-temperature——- curve fell. At the same time,. a part of the light. energy_.degenerates “into heat; raising the témperature of the sus pension and the cell. | “The -more—intensive the photésyn thesis, the less ‘absorbed light gece nerates—inte—heat;-~ Ne ~~ ; at is, the more intensive the photosynthesis ,. the "colder" ed-growth-witl beduring the remaining equilibrium ~condt tions.—_The energy ou tput—is—eateul ated-according—to-the—__— - oll owing formula: ~the-iltuminat g/séc . : .P? is the inte *. gral thermal effect at—the-moment—of—determt ~™@ action of the 11 he ht, K is the heat capacity of the ca calculation of .the qua ~~ une eee ntum emission (output) of opr @Sséd aS f7a, where r= ~ 2"-the-en nation—during ! i1-at—the— photosynthesis the amount of emitted oxygen in moles, oo eral Stgy of the optical radiation in Einsteins of absorbed. , noe Ss We-find—that—thts amount correlates with. the magnitude of. ~ Tt asda eeeutithe—quantum-output-of pinto synthests during Taser Light Appreved-For Release 2000/08/07 : CIA-RDP96-00787R000500 Ma 7 USIB DEPARTMENTS ONLY \~ Y=