———"_THE REACTION OF ENDOCRINAL GLANDS DURING RADIATION=....=2Z ____-consequently,—such—an-important—gland_of_internal-secretion—as—the——__ uperrenal_happens_to_be_ subjected to more marked changes in function under the influence of coherent monochromatic light, 6328 A , in comparison with a less monochromatic, noncoherent radiation with {ts —aximunm at about 6400 4. | __-- Fhe-stimulation—of the—-superrena—is—indieated—by—biochemical—data | ag -well—as—results—obtained_during histological _and_histochemical— research. The data. _prompted_the thought that laser light is u o res tilizible as an anti-inflammatory treatment. At the present time, _ our hypothesis has obtained the support inclinics dealing with a whole series of ailments. OOOO ex with-doctor-0.A. Zavyatov-at-the-ministry—of-heaith——___ | otinteal-hospital_ofthe—Kaz akh—SSR,—we-. .-observed—Jo-patients—sufferinc | __ from rheumatoid_and_transformational_ distrophic_polyarthritis. Of | theses 7) distrophic polyarthritis, -Avong those. and 31 from trans- formation distrophic polyarthritis. Among those suffering from rheumatoid polyarthritis, theré was a préponderence of women, ages {|__ae ~65-years, the-majority,—30;—having—suf fered-fo r-more—ty —5—years--— hey—had-many—times—re ceived—treatment—in-clinics—and -health_resorts_ _without—any_noticiable therapeutic effect... At reception, the sufferers were subjected to a whole complex of clinical, biochemical, and X-ray examinations, confirming the diagnosis. According to the degrée of activity (according to Néstérov) and the process phases; the-sufferers-_were-calssified—as- follows: —activity—of—first—degree—(in-15),activity of_2nd- Land-third degree — __(in 24), acute exudative phase, in 1] patient, sub-acute_exudative croliferative, in 33 and fibrose-sclerotic in 5. patients.-..- . The sufferers received treatment from a monochromatic red light Source radiated from a prototypé He-Ne lasér, LG=75, wavelength 6328 Al Concurrently; —S ~pattents were given-massage—and-phys io———_—__ —therapeutic-exercises.—— @-@3———_______________________. --__-___-The_radiation was_of the general _ local _characeer_of_activity_on __the damaged joints and reflexo-genic . 1 zones, applied in physio- therapy. Radiation exposure varied from 1 to 30 seconds on each area, and was selected according to the initial condition of thé patient; ~ “the phasés and dégree of process—activity.——— anne " —-———-—-Some-patients—experience—a-slight—vertigo,—sleepiness,—and—_ ~— increased-perspiration_after the first 2_or_3_sessions.__After adaptatior ~-to_.radiation, these symptoms disappeared. After 3-4 sessions, a_ ‘| _ shotoreaction was observed in all of them. In this period, an increase __.in_pain in joints was observed (in all the patients) the appearance ~~ = swelling joints, (in 3 pattents), the increase in temperature —(—5-— t ~ pati ents).—En-one-pattent,;—a slight—inerease_in-arterial _pressure—was- _ --registered.—tIn—this_period,_radiation was prolonged, but with -. Secreased exposure, In 2-3 days, all the effects dampened down. towards the end, the ' pain symdrome decreased in 35 patients, the _exudative phenomena died down, the area of Joint movement Increaséd, — “Orming stifinéss Was Tess 7 posed > re nt No marked changes—we oted-in—the-hemogram.—_The-ROE—became si ightly—more—rapid_towards_the-middle of the course, returning to. F-- = tS-original value towards the end of therapy. Towards the middle of _ —T"S_sourse, some decrease in lymphocytes took place, levelling out ~~ Soeards its end. The S=-. reactive of white corpuscles did not change - cur ia g therapy. The fibroginogen increased towards the loth session, and decreased towardsthe—2oth; remaining -within—the-nerm.—The-contnet *—albumin—inereased_somewhat—in the middle of. therapy, and __ ession decreased to its initial level. Towards the _ pie SAREE Sore i: ; oJ yf = : 8 ESA AS ee ee ROR DPIGDO7S7R :
oo —_ ____ Approved Fer Retease-2000/08/07 : CIA-RDP96-0078TROOOSO008000T-0- __——"THE REACTION OF ENDOCRINAL GLANDS DURING RADIATION---3 __ _—_— —o ut-of-39-patients;improvements were observed in 35. The ~~ _____-polyarthritis—and_multiple-ancilosis—(bending -ability-of a~joint — ~ | ____ to _Move_AS_a_re sult_of _the_growth in joint -swellings---Eds}-and-in 1 patient with exudative proliferative phase process, who_violated the - ———regimen. We did not observe any worsening of conditions of negative | __—ananges in the joints of a Singlé patient... Mitder, delayed resutts were traced“in 21 patients.” Of them, 19 | __ with-exudative-proliferative-changes;—a .  “s-decrease-in-joint paind ' was_achieved, ,_an increase_in-their—mobility--—a—decrease-in-morning:: [_stiffness, compared with the initi al_condition.In. these.patients, , a good therapeutic effect was . obtained after the second session. _ ——or treatment. Af first, no improvements were observed in 2 patients, -——_—- with fibro-scterotic process phasés 7 | the —-dynamics—of-the-amount—of—leucocytes~and~ROE-in- the-second | __ course_of treatment did not—change -—_Atendency—for—the-number of.----- [__lymphocytes to increase towards the 2 oth_day_of the_procedures.was (observed. Apart from that, a decrease in the content of general (white albumin and alpha-globulins was observed. The S-reactive of +-——-whtte -corpusices did not change: TOT ae EIn—the—-group-of-transformational-distrophic polyarthritis patients were 17 women_and14-men,—aged-between—35—-65-years- with—a-case . history of more than 3 years. Of them_21_with_nerve.osteo-arthrisis | primary and lo with distrophic damage to joints of_different orgin:. changing, hormonal, professional, and others... Treatment of those - pattents-was-the_ —same~as those suffering from rheumatoid “= =~ ~— ——-polyarthritis.—iIn-this—group;—an—analagous-phtotreaction was observed. _.___ However, the > pain_syndrome—decreased-more—rapidly—than-in-those--- suffering from rheumatoid polyarthriétis._Also, towards the end_of -. the course, mobility was increased. (area_of movement). There were. improvements of greater or lesser. degree in all patients. Hematologica data reflect the tendency of the ROE t6 decrease. The number of leucecytes—increased-somewhat-towards- the middle of the course,;"~~° ~ __ thereafter decreasing to initial-—values.—The-number-of lymphocytes .__increased within normal_bounds. ee A.M. Moshcheyeva of the ministry of health Kazakh SSR clinical “““héspital investigated some biochemical indices of blood during ‘Taser radiation stimulation in 62 patients, suffering from inflammator- r-——processes-of-the- _—ss§- #-uteral~appendages.—The-dynamics of the white + —-corpuscle fractions—of the. blood-serum-was—determined- with-the | use: . - Of _A.E. Gurwich's_method.__The_ electrophoresis..was_carried_—out-in __.a buffer medinal -verinal solution of Ph 8.6 with a_ ___tension _ (potentfal) of 300-V. . The data obtained was quantdtively deciphered F-—"on~arl FEKM {(photo=éléctron microscopé ?--Eds). Concomitantly, the — F ——quantity—of-generai-serum white-was-determined-by-the biuretic method, r-—rodified_by L.I.-Slutskiy{1964)}--on—a—spectrophotometer-SF-4R.—- ----- »-——(Note: a biuret reaction_is a chemical test_used_for..proteins—in—— -- __ SOlution. Employs copper sulphate in_akaline solution, which gives . pHegle color with proteins and with a few other substances---Eds). _ __; The first group of 31 subjects comprised patients with an acute -orm-of—chronic—inftammatory processes taking placé in the tteral ~~ ~— appendages.—The-second—group-were-patients-with—inflammatory processes + An_the chronic state of illness, 31 subj ects == ——_, In .-the control group of healthy women, the _mean_level_ of general whife content was in the region of 7.90 £0477 g % which corresponded to data fotnd in the literature. Yn the’first group, the content of senerat white-serum of the bitood in 28 patients was within normal Approved Fut Retdasé 2099108/07 b2IA-ROPSS-DO7S FRG0GBE008000150 2-6: 0-78). {
See ot : 2008eeeeeee eee ___-- THE REACT ION—-OF-ENDOGRINAL—GLAN DS—DURING—RADIATION —4———____-— ——~“The concentration of serum white corpuscles _on the ave rage_comprised__ 7.69 + 0-096 g % which was somewhat below the indices of the control _ ———group. If the indices of the general white corpuscles of blood serum | ata rot show simular rises, then-the separate white corpuscle fraction | showed—marke d—changes.——_White—corpuscles-were—observed--in-only—five —— atients, while, in 26 of them , a decrease was established, If. the mean index of albumin content in the men of the control group comprised 4.68 + 0.042 g %, then in the group with acute chronic inflammatory _ = it was considerably lower: 3.98 + 0.98 g % at P Tess than [Wt th-the—an alys ts-of-globulin—indices-— of-serumbiood—fractions—a— \ nsiderable increase in the content of alpha-=2 and gamma=globulins—and [1es s pronounced increases in alpha-l_ and beta-globulins_was. -° _ [——gstablishea . With the determination of the albuno-globulin indatator " [—_eoefficient, its decrease was discovered in comparison with the norm, ——_which points to the sharpening (worsening) of the inflammatory processes [+ Under the “infiuence of 74 aser—-radtation-therapy;-tog ether-with——~ _a_clinical_improvement—genuine—changes—in-—the—content—of-the—various. — albumin fractions were observed, which pointed to_the cuperation_of the inflammatory processes. _ Thus, towards the loth procedure, especially towards the end of reatment, a consistent tineréase In albumin content Was observed, the — ecrease—in-ati-globulin—fractions;—especially the; alpha-2 anad———_ _.gamma_fractions.__._ The _albumin=globin-coefficient—index.attained-a———- characteristic value for healthy women, in practical _terms,_1.34 + 0.041 ~ As was expected, the inflammatory process in the chronic stage _ of iliness presented a different piciture. In 20 patients of that group, the index of gerieral albumin wads so6mewhat Higher than the mean” index—of—the—controi—group;—and-only-i- patient manifested -an_instont fice hypoproteinia—(6.90-g-%)._lfi_during—aggravation-of the—inflammatory process, a decrease_of _ the albumino-glohulin_coefficient was_noticdad,_ then in the inflammatory process of the chronic state of illness it ~ was within the normal bounds, 1.56 + 0.047. ‘Towards the loth procedure, —paralier wtth the clinical manifestation of the aggravation of the ~~ —-—infliammatory-~ process; a certain-decreaase-in—albumin-content was————— ——observed,—the-increase-of—all_globulin—fractions,—and-corresponding——- __decrease_in_the_albumin-globulin_coefficient _(1.27._+.0.048).. Towards _the end of traatment, a normalization of the albumin content of blood. serum manifested itself. Albumins returned more rapidly to normal } an ad ailpha=2 and gamma globulins moéré slowly. "A tendéncy towards” ~~ " ——the normalizations—of the albumin—coefficient (134-4 0.048) was" -——-opserved ee In_this way, research made into the albumin fractians of blood _ | serum in the various stages of inflammatory processes of uteral . _ | appendages as well as the influence of laser radiation therapy enabled [the statement of the following principles: f——1)-The -serum-protein changes taking place-during-inflammatory—processes r——in—the-uteral-appendages—reflect—the— _ -aeuteness—and-severity———- | of _the_disease_process._The _more_severe_the process, _the_lower_the ~—quantity of general albumin, the lower the albumin--globulin index the_ higher the content of globulin. Patients with an acute form of chronic inflammatory processes of uterat appendages subjected to-taser ray therapy paratlel with-chinical [_*ecovery,—were—observed—to-reach—a -state_of—normalization—in—their —albumin | : r content, general alhumin, alhumin-globnlin coefficient, and decrease in globulins, especially alpha-2 and beta fractions. 3) Towards the loth procedure of therapy interferences in the blood | albumin spectrum were observed in the | inflammatory process of chronic - DPIGDOT 7 RUGOSe0CS0G0F-Wut. of the
sé 2000/08/07:: :CIA-RDP96-00787R000500080004-90-—____ _____Approved For Retea I _—_— THE REACTION_OF ENDOCRI NAL—GLANDS -DURING-RAD IATION - ove 5 earlier rises in albumin fraction contents were_obse rved, e ministry of health Kazakh SSR_clinical__ _____—_hespital -carried-out He-Ne laser therapy on 68 ill women, among them. |___30_suffering—from—acute—form-of—chronie-tn flammatory: processes..in the uteral_appendage S—and—38—from—a—chronie-stage—o f-inflammation. | ___$— Fe omen suffered from _a_tw =Sided_inflammatory—process;—and-24-ftom- _ a I-sided (i.e. bilateral and und lateral---Eds)._Their—ages—varied— from Z0-43 years. ——— fhe duractioepf tiiness varie from 5S_months to. 17 years. The _ eof _tliness—in—40-women-were the results of_abortions, in 9, the resuit—of pathological_factors;—in-12; the provoking £S6tae Was. chills, in 7, causes could not he dete rmined.- ‘Prior-to-entering -the fT elinic, they complained of pial of general deficiencées, lack-of sleep-and—— -———— appetite, Irritibility, perspiration, , constant or _periodi¢ pains_in. the—lower~sectors of the stomach, and loins,.. __ __t - with —irridations—of-pain-into-the lower Limbs, painful, abundant irregular_menstrations,and oo -paleness; : OO 30 patients manifeste d-a_sub-fibril—(ie,-below-fever---Eas}—— [body temperature. 32 women suffered. sterility. po ALL the patients were subjectdd to clinical and sometimes taboratory tests during thé tharapy. process. Laser ray therapy was carried-out—according-to~a-method-worked-olt by ws -,- Laser light FETS Gave enn ted out—on-a-specially-constrycted Wé=Né—Lasee _-LG+75, wavelength 6: 328 _A, intensity 25 mW/_om’,____ ~ The Length of radiation on definite _reflexogenic_zones-and | ———~knownattipufictural points varied according to the reaction of the [organism-to-the7the i Yapy applied... ; ,———_The-total-single—radiatton-exposuré lasted 7 from lo-15 minutes. rom lo-15 minutes. The course_of therapy from-20-25-procedures>—w hich-weré carried out daily at exactly the same_ti- me_.__ pat a SMS The patients did {1 not receive_medicaments_either_before-or-after— subjection t6 radiation. tn 30 women, _ after ‘the fourth to loth pro-_ ~— cedures;—the—acute "stage Of chronic inflammato .- — pteral-appendages~showed—an—improvelient in génerar condition; -—_-in_pain below_the_stomach—and—. J-toins;-normattaatton in body L- romperature. a During_this_time,—a—numberical—increase-inrthe—tndex— _ of the siat cid was observed.” . Although stattctiontde -it-turned —— - [~~~ 6ut7€o Be insignificant. Analagous results_were_also_obtained_ by us— p-—~ in the—indtces“of “s=¥aacer ve albumin content. Towards the end of _ + therapy--—together-with—the-stontficant ¢linical effect, a genuine . ... Geg Teasein_the-index-of—the-sial acid -was~observed-in the 25 pati . .._Which pointed -fo—the_cuperation_of—the—inflammatory-process+— Ae fee ~ 28 the S-reactive albumin index_is_concerned,we_did-not—discover—any- ga ver gences of its content practically speaking, in any of the-healthy . 77> women>—— = thus —~after—thetermination-of thé -£LFSt c oy of . Dherat so Suffering—from-acute—chronto—inflamiatorp processes in the ~——Uteral appendages, a_ »total_therapeutieeffe ct-was~attained by 17, i 4 partial effect by 8, with no. effect _nated_in 5 women.—13-patients—- who did not obtain th erapeitic effect were recommended_to— ~ ~bake~a-repsated course Sf Ieee etching from _ lo-15Bittings | "pp 3cmonths~after-the-first course APeeeee Second course ended, ~-—lo -Out_of the-13-pati Sata obese? # full eliggcal 6: ffect, which |_.was_confirmed by the date chismed a ttt sia acid-and-the s=reactive = albumin, sufi cifect was absent in ‘3 patients who were later fouaue | _ Rave a plosalpincs in whose cases operative + therapy was neccessary, | _ ‘Noter fromthe Greek ipion Gat say spion, and salpincs--~tube, i.e, "rotting tube" _ or Spi heres coltectton of rot in the uteral tubs Which ends, 0 _PSApprSved' for Retease200e0867--CIA-RE -__o Freee oes ne cee es ”:”*~<“‘<‘—«é‘“«;é«~rS SE DEPARTMENTS ONLY. - --
____-THE—REACTI ON—OF—ENDOCRINAL—GLANDS—DURING—RADIATION +++ ~6 ! _ Out of 38 patients in the chronic stage_of illness, towards the _ [Toth procedure, 22 were observed to undergo a .‘ worsening of general _ condition, and Intensification of pain below the stomach and loins,an | [eo evease in albumin; lack of steep and-arporttes tie foe teettee feverish—body—temperature—appeared,.—__________________________ | ALI this pointed to the _intensification_of the inflammatory proces of the uteral appendages. After the first course of treatmeht.27 out. | —tF the 38 women noticed a disappearance of the inflammatory state rand pains in the regions of the uteral appendages. The latter were - eat _patpation._7 patients obtained a partial effect, a certain __—_decrease—in_the—dimension-of—inflammatory—formation£-of-uteral—appendage. | decrease_in_pain_at-palpation..—No_effectwas—noticed—on-4-patient: When analysing the indices of sfal acid_in the process_of_ therapy, a [noticable increase towards the loth procedure was established. In in _ some patients, it lasted until the end of the first course of therapy. Pe io ede rd re ane eed ae a |-—_pointedto—the—aggravation-of—the—inflammatoryprocess~—Towards—the— } middle of therapy, the S-reactive albumin _indicator_had—a_tendency—— . | to increase somewhat, however, statistically, it turned_out_to_be ["insignificant. 11 Women in the group which did not, obtain a full effect underwent a repeated course of laser radiation with. good results. Investigations made—into the dynamic chatiges of hendgrams in the-process—of—lLaser—light © —th 7 Thad ned lnerie tif [—grovpar to signisicant tncressesvin the quantity of leucocyte contnet ~y groups.—No-significant—increases-in-the- quantity—of-leucocyte-contnet was_established._The reactive—index-of_eritrocytes—sedimentatLon— | (ROB) varied somewhat_during the _process_of treatment, however, these | changes were within the bounds of physiological norms. ee - When dealing with the white blood diagram, it is neccessary to ” - Observe that towards the end of treatment, the quantity of néutrophyles — definitely decreased in pattents-with-chronic-inflammatory processes in -—_their—acute—states. —_——in_the_majority of patients of both groups, together.with-their _ clinical recovery, _a_ certain increase in eozynophyles_was_.observed._._. (Note: eosinophil leucocyte: polymorphonuclear leucocyte of vertebrates, [ reontaining granules staining ts acid dyes such as eosin. In human | ~ beings normally ahout-2~5- ofall leucocytes” but become much” increased + ~in-certain—parasitie—infections;—and-in-—allergies—--Eds}~ + The, changes_taking place corresponded_to-the—clinical -recovery— »_of the patients. We investigated the long-term results of laser radiatic ___therapy in 53 patients over a period of 5-12 months. Over the period mentioned no symtoms whatsoever.“  <were (observed —in-any ofthe women, pointing-to-the -extstence of an in flamnator ~—~process-whieh—is—confirmed-by—elinical-investigations—of-data;——_ i_women who earlier suffered from _sterility,-became-—pregnant.
——— pproved For Reléasé. 2000/08/07. : CIA-RDP96-00787RO005600080001-6 - - -- - - - -- __ Chapter V_._Stimulation_of regeneration hy monochromatic : red—light- y the local _ action of polarized MPRL (monochromatic polarized red __ ght}—waveiength—from_t300-6400-A-on—skin-wounds—(lesions) distribute: n_the-ear_of a_rabhbit, a.characteristic_histologic_ change was observed which pointed. to the stimulation of regenerative processes. This was done by B.L-Koritniy and collaborators in 1967. Although the experiments were Carried out on the samé rabbit, oné éar being radiated by iorochromatic polartxed lighet and the-other servin purpeses—of-eontrol,—distinct*merphological—changes—manifested——— - themselves-_under—the action of red light. And so, in the wound waich was subjected to radiation, already within'a period of three hours, a. larger number of neutrophyles began to appear than in --—-—the control ear. In succussive periods, an ihcréase in the in-_ tenstty of phagocytis wasn ted.—The bactertai-flora-compesed——-—— 9 £-diplo~and-tetra-cocei—aimost—entirely—phagocytosized—in—the ——__ __._ wound_exudate._A_more-rapid healing of wounds _was_registered_ when monochromatic polarized red: light radiation was applied. At the ae ee basis of the observied:effect, according to the authors, lies the “"“Ré¢ivation of the transformation of properties in the skin during —— “-sehe7Tocal activity of polarized red tight.————_—- —————_ oe A lLarge-eycle-ef—werk—was—earried—out—by- prof,._D,L,-Koritniy,——___ explaining some_of_the_ morphological and histological displacements_ .n the auto-transplantation of. the skin of a rabbit during the action of monochromatic red light. It is curious that during macroscpic ” “cbservations, a véry distinct difference in the condition of transplanted skin tshreds}-ts-engendered -with-hardly—noticeabie——— eniererlings—ttri pe;—intengtines).—Duringhistological—trials— sharacteristic. hanges manifested themselves already in the course _ £ the_first days after. radiation was initiated. The: leucoctytal all in the radiated: skin shreds was considerably more prominent nm comparison with those that were not radiatéd~ During the activity” .£ monochromatic polarized red light—an accetaratton-of inflammatory —— hs | a s9- phase-changes—oceur,—and—the—proliferation—of—fibroblastic — fy 0 ¢ nts—is_stimulated,Characteristic is the absence of fibrose__ 4shments.(nutrients) which in unradiated auto~transplants ar_as whole.separate areas. . ne same time, on the. background of thé stimulatonn ‘of connective lesa slements7 the re“eptdermation of the radiated-transpiant—-—- t tliws mown. Onlytowards—the—twentieth—day—does_the-whole_transplant— b * 4+ neceome_covered by the epidermis created. In radiated_transplants * " “stents of the epithelia along thecegndective tissue slows down, (+Fison with the control samples, for example by ten days. n the restituted: phasé anew épithelial Covering of the — - ransplant manifests—a-quickening—tempo-of-its-productivity. od-of-one-month—after—the—transplantation-the_organo=__ tructure of the radiated transplant "“- -reestablishes _ the_same_degree: as transplants not subjected:to the activity ‘Fomatic polarized red light do in a period-of. 2-3 months. € +" WY SY am (7 * « acer ~ . ° ° a? oe be eee le had ny! e266 toa rote ‘@ analysis of glycogenes in the epidermis; it has been mst under” the tinfivence-of-monochromatic-potarized-red—tight . xe oc of-—ten—days—tiex24—-hrs.}_an—increase-in concentration. ~s95—-this,—a_decrease.of glycogene concentration in the eeolegat ¢ we : bane 3 ¢~.sxin_shreds takes: place, when compared.with. control a 3 fe dm rt : t wNich is explicible by the more greatly accelerated tempos 8 @ +o a, “pita a ~ ~ i - t)Tanteeg-oeeerentiation. — topge - ‘= “ ANK” change-refiects the-entire-regenerative—proc ess— Te Srese; sf CRS cheration, then ente trans piantation,—In_the first lo days A-RDP9 ae led (a!
~- ~~" “Approved For Release 2000/08/07 = CIA-RDP96-00787 Ro90500080004-9—______. stimulation of regeneration by monochromatic red light. -2 exceeds_this in the control sample, in_this_case, twofold, ‘gecording to D.L-Koritniy, the RNK concentration in the epithelia cof the radiated transplants is relatively lower on the 2oth day and ———"_jfg even ower on the 30th day aftér transplantation, this being —— imed— y-the—increase-of-the morphological processes—in-the——_—_" |  epithelial—plast,—It—follows—that-monochromatic—polarized—red—light— | ut_to_he_a-powerful stimulator of regenerative processes in _ wounds as well as _ in freely transplanted skin. Phe next. experiments were carried out using coherent red light. In @ republic clinical hospital of the ministry of héalth of the ~~ gakh soviet soctattist repubtic inthe -biophysicat taboratory—o ¢— e-Kazakh—-university—and-in—other_medical-biological_establishments- erable _amount_of factual material _has_been.collected demon-_ r stating the possibility of utilizing the biostimulation of physiologi- eal processes with the aid of monochromatic light radiations from He-Ne lasers. Some therapeutical aspects of the effectiveness oF ~_ such btostinutation vere tearneds—HHJ i WHWV_NHw"y_sco— nn — eee ___-_—_- Be low, -we-reveal—our—experience—during- experimental _work—concerned——_ withthe stimulation of regenerating processes by the radiation of _ He-Ne lasers and our clinical work when we used low-power. lasers for 7 the treatment of some ailiments.. The rapidity of the process of regeneration, its stimtlation, or” _ ——_—_tnihibttionts—a—_good-indicator-of-the-activity-of chemical or-physical —agents $V -— a _*_aesuming thatthe. endogenal. ray regimen_of mitogenétic radiation during regenerative processes manifests itself as one of the con-_ ~~" ~“@itions. determining. the proliferation of cells, we carried out a series Of expéiménts to éxplain. the possibility of stimulating ———_ ——regenerations by taser _tight-on-account-of the-induction of-secondary ————radiation—in—the-UvV—waveband.—The_possible-aspects_of-applying _ low-intensity He-Ne laser light.for. the regeneration of bone tissue _ _ were learned. The experiments were carried out by.P.P.Chekyrov on 72 mongrels. The regeneration was studied on the radius bone when it ~“"“""was Sawn. (partal cléavage) and total cross=section fracture. According / ——~to-bibltographical data during-the-trauma-of the-radius— bene; —its-— -——-regeneration—takes—place—according—_to.the_first mentioned. type._At .-__-total_cross ‘section _fraction_of_the radius bone_the second type of _ __.regeneration predominates, with thehppearance of bone oVergrowth (bones. becomes thicker). The operations Were carried out according to “strictly standard methods in adseptical conditions under general ——— "narcosis. The depth _of-the-surface-sawing-was- measured-with-the-aid— _ ~—of-dividers,;—The—wound-was—sewn-up-hermetically after which an anti= ~-—septic_bandage.was_placedi To attain_the_total fracture of the radius bone, an electrical cutter was. used, the cut made was equel ew to 4/5 ths of_the.bone. diameter, after which it was-broken with the aid of mechanical force. The rémaining opérations were the same— - | as inthe -ttrst case.— OO f  ——On-the-plaster—of-paris--binding,—at—the-siteof_the fracture an —_ -—-—opening—l_cm_sq in diameter, was made, to facilitate radiation by -—---laser_light, The locality of the fracture was radiated “with coherent - monoch’romatic light of the optical quantum generator. wavelength 6328 A of intensity lo mW/cm* (Diagram. 3 > Caption: external view of He=Ne— lasér for the purpose—-of radtation bone fractures—and-wounds},——_—_. . ~~ Radiation-was—earried-out—in_conditions—of -darkness,—without additiona: —— i Llumination,—Exposures varied from l-lo m nutes» Observations were _ Ge_in peiods of 60-90. days after the trauma.was:made. In those eriods,.all dogs underwent. roentenography of. the. radius bone, comparing the. roentgenograms. obtained with thé control samples~———— - _ Made during. the same. period’ of observation, the following was-observed | Approved rR e- 2006/08 re
-_—_—Approved For Release : stimulation of regeneration by monochromatic red-iight:-~ 3 nnn SSE a ___on-the- X=ray—of—the—control..dog,—_the_ saw_cutting_was_still_ observed _ at all _ stages but _showed_small_contrasts,. The edge of the bone opposites the cutting, an area of. peri-remnant bone thickening was formed.  __ This peri-remnant covering-has its greatest.density on the circumférenc Without having to go” “into detatil\ed dtiscriptions --of-other-experiment possibte-to-summarize- the-resuits—of-the—-experiments.—The—de gree of healing—ofthe—bone—_thickness—in—the—experimental_dogs_was_consideré higher than in the control dogs, Thus, for _example,_the_first signs ; of development of bone thickening (growth) in the: control dogs appearec. - towards the 2oth day, whereas in the experimental dogs, towards the oth day. On the Zoth day of observation, thé bone overgrowth tn the experimentai-dogs _appeared-in thd@form-of-a-protuberance—on—the—opposite side—ofthe—surface—sawing—of—the_radius_bone._The_sector_.of_the--radius pbone_in_the area_of_sawing.was_compacted (fidled_in). Towards the 6oth day in dogs of the series. mentioned, the effect was already unopserv- able, whereas in the control dogs, it persisted at the edge of the ———Yyadius bone, . . By—comparing the X-rays—of dogs-of—both—series—on-the—-9oth—day,,—it—— was—established—that—in—the-experinental_dogs,_a_total_regeneration_-_ _of the structure of the_radinus_bone_took_place, in the control group, the localitu of the sawing was visible in the form of a thickening ; —~o¢ that part of the radius bone. Histological. experiments also —_ ———point to thé indtbifablie effectiveness of stimulaing—lasers—on bone— ———— regeneration. —__———_ 7 — ——What-—is—the_mechanism_of_such.action? Does not warmth influence _-__the_rapidity of the_regenerative processes? ({ Diagram 4:-X-rays of _ the radius-bone of dogs, 15th day after fracture: A.. control group, B.. stimulation by laser radiation). | Putting the question in this way-is apparently insolvable, since thermal effect cannot contribute its heating effeet-to—biologicat— ~—activity—in—econnection-with-the—-faetthat -h ardly—any-heating-of — --——-the tissue during radiation takes place. For example, during an exposure of ten“minutes, at radiative piwer lo-12 mW/cm'sq. the local. heating of the tissue all-invall comprises from 0,5-1l. degree centigrac: | - Such a. thermal heating can hardly bring “about stich manif est physioldgi- ~—~catl_and morphotogicat- changes “the -regenerating—-area:--At-the-same~-ti- ~—-— one -shouid-not—totally—refute—that—thermal-phenomena-do.-not—manifest. a a—certain influence on capillary - trophic. surfaces.._However ,-such._... activity is not.so.manifest.as.to influence the speed. of regenerating processes so that one. could be in a state to observe tiem in our experiments. ~~ it ts "knownthat-warm-baths;-and-suchtike;-heat+tissue-by—5—lo-degrees r o-~-€-and—-mere,—By—in ereasing—the—temperature—of_radiation,-acting on —-. ~-—the_contrary_an inhibition of the ‘regenerative processes. take S place. --__At_the same time, ‘during. the. action of. laser radiation. of. low intensit: _.__4nd_ exposure, the increase: in temperature. is several-times. lower. On'the other hand, during. the sane intensities of the “acting factor; ___nO-distinet stimutating-effects-in-the-dark-red-part-of-—the—spectrum- ” ~~ 8re—observable,-which-confirms—the—frequency=dependant. ~——~Saaracter_of the activity of the radiating_agent, in particular the _ - He-Ne laser of wavelength. 6328-A. This. demonstates- the resonance - =—-SeSt Of wavelength O3<8°A, This. c mechanism of. action of the. given factor. __We suggest’ that the basic matrix resonting-on-the-actton—of————_- _ Nenochromatic;-coherentradtation;-is-the bioplasma; Hewever,—before : so=ng-on-te—consideration—of—energy—changes- Jasma_subjected ~——to_ 43 : ty of radtation, it would be: expedient to'review a whole Series of other clinical and. experimental. data, demonstrating the mon~thermal. character and at the same time the- highly stimulating Ne- laser radiation. action of He-Ne-laser radiation. —