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a major cause of mortality in AIDS.
P. carinii and AIDS have been attracting the interestjs
and attention of many researchers
all over the world. Studies of P. carinii by many methods
and different ways have important significance. The paper reports for the first time the effect
of the emitted qi on P. carinii infected Wistar rats.
j
I. Adult female Wistar rats weighing 150~200g were divided into A (26) ,B(28)
, C(26j)
groups randomly. Group C was the control one.
Two qigong masters emitted their qi to Groups A and B for 15 minutes each time oncle
every other day, 7 times altogether. Then P. carinii Pneumonia animal model was built up by
a regular method. The rats received a standard treatment regimen of cortison acetate.
25 mg ,
injected subcutaneously twice a week, low protein(7%) diet, and tetracycling in rat/s drink-
ing water until examination.
After drug treatment started, the rats were treated by the emit-
ted qi for one week
. They were examined 4-8 weeks after the drug treatment.
The experimental results suggested that the body weight of the
3 groups decreased
, but
this; was more obvious in the control group. Table
1 shows the difference of the infected rats
among the three groups. About the carinii infection rate see Table
1.
For differentiation of infected degree, see Table 2.
It shows the difference of the infected
degree of P.
carinii among the three groups.
Table
1.
Infected rate of P. carinii
group
counts
PCC+)
rate of infection
X 2 test
A
65.4
A
* B P>0. 05
B
50.0
B
* C P<0. 01
c
92. 3
A
* C P<0. 01
;
Table 2.
Difference of infected degree
counts of PC(+ )
infection degree
heavy
moderate
light
A
17/26
•
B
14/28
l
C
24/26
;
The lung smears from the three groups were stained by Giemsa. It was found that thk
parasites were the typical P. carinii. Table
3 shows the differences of the sizes
of P. cariniii
cyst among the three groups.
Table 3.
Differences of sizes of P. carinii cyst
PC from
counts
diameter(X. ft)
SE
t test
A
100
4. 78
0.6518
A
5 B P>0. 05
B
100
4. 98
0.4980
B
» C P>0. 05
C
100
4. 74
0. 5326
A
* C P>0. 05
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The experimental results suggest that the emitted qi reduces the infection rate and de-
gree of Wistar rats by P. carinii. The emitted qi can inhibit the infection of P. caiinii.
I
. Nude mice were divided into two groups
. Group A(10) was injected of 5-8 X 10
par-
asites by lung innocubation. Group B were injected of parasites which had been treated by the
emitted qi
. The parasites were put into a small glass. A qigong master emitted his
qi to the
parasites 20 minutes and altogether 4 times. Each interval lasted for 48 hrs.
The experimental results showed that 10 mice of Group A were infected by PC, in which
4 mice were of moderate infection degree, but in Group B only
6 mice were infected by PC,
and all were of light infection degree. Two groups had big difference.
1-44
THE DOUBLE-BLIND TEST OF THE EMITTED QI ON
TUMOR FORMATION OF A NASOPHARYNGEAL CAR-
CINOMA CELL LINE IN NUDE MICE
Chen Xiaojun
(,\Yi Qing
(1) ,Liu Kela a>
, Zhang Jinmei
(2) ,Chen Yu Sheng
(3)
(1)
Cancer Center ,Sun Yat-sen University of Medical Sciences
(2)
Dept. of Physiology ,Sun Yat-sen University of Medical Sciences
(3) Qigong Association of Guangdong , China
Following the double-blind method,we carried out two experiments by using cell culture
and nude mice.
In the first experiment
,a qigong master treated the poorly differentiated na-
sopharyngeal squamous cell carcinoma cell line (CNE-2) with his emitted qi
, followed by the
inoculation of the treated cell (qigong group) and the untreated cells (control group) into 16
female nude mice (NC-Z strain mice). The results showed that the tumor formation of the
treated cells was inhibited by an impressive rate of 100% (3/3). The same procedures were
followed
in the second experiment. The results demonstrated that the inhibiting
rate was
still 100% (4/4). A pathological examination further confirmed that there were no tumor
cells in the inoculating site of the qigong group
, whereas poorly differentiated squamous cell
carcinomas were observed in tumor of the control group. No tumors were found in the livers
and lungs of the mice in the qigong group. But in the control group of the first experiment,
tumors metastasized to the lung in mouse 2. No tumors were found in the liver of mice in the
first experiment or in the livers and lungs of the other mice
. Although the mechanism of the
effect of the emitted qi still remains unknown, the results of the present research reveals the
necessity as well as the possibility of further investigating the inhibition of tumors and
its
mechanism.
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1^45
AN EXPERIMENTAL RESEARCH OF THE INFLUENCE
OF THE EMITTED QI ON CANCER GROWTH
, METAS-
TASIS AND SURVIVAL TIME OF THE HOST
Qian Shusen,Sun Wei, Liu Qing,WanYi,Shi Xiaodong
China Rehabilitaion Research Center, Beijing 100077, China
In researches of the influence of the emitted qi on cancer growth , metastasis and survival
time
of host, 114 animal cancer models were used
in
three
batches. Tumor models were
formed in mice by transplantation of U27 or MO, cancer cells into subcutaneous tissues of the
right armpit respectively. These mice with cancer cells were divided randomly into the qigong
group and control group and all mice of the two groups were fed with same food. From the
second day or seventh day after transplantation of cancer cells, the mice of the qigong group
were treated for 10-30 minutes with the emitted qi every day, but the mice of the control
grodp were not given any treatment. In experiment
1 ,on the 20th day after transplantation of
cancpr cells, all mice of the two groups were killed and all tumor volumes were measured
inJ
dividually. In experiment 2, on the 23rd and 33rd day after transplantation of'cancer cells, all
mice; of the two groups were killed and all armpit lymph nodes and lungs were taken out indi-
vidually and examined histopathologically. In experiment 3, when the mice of the two groups!
died, their survival time after transplantation of cancer cells was calculated respectively.
The following results were found-
I* In experiment l,the average tumor volume of the qigong group (31 mice, 2. 25 ± 5 .
i
35cm
3 )was obvioulsy lower than the control group(32mice,6. 32±10. 02cm
) ,p<0. 001.
2. In experiment 2, the metastatic rate(l/16)of lymph nodes
in the qigong group was:
significantly lower than the control group(6/15) ,P<0. 05.
?. In experiment 3. the average survival time (35. 4 d)of mice with cancer in the qigong
group (10 mice)was obviously longer than the control group(10 mice, 30. 5d),P<C0. 002.
These results indicate that the emitted qi can delay cancer growth .reduce metastatic rate
and prolong the survival time of mice with cancer.
THE EMITTED QI ON MICE TUMOR PREVENTION AND
TREATMENT
Feng Lida, Peng Liaomin
Chinese Immunology Research Center , Beijing
Through random division, we had 30 mice divided into three groups -the tumor control
group (
I ), qigong protective group( I )and qigong protective and treating group( I )to look
into the use of the emitted qi in tumor prevention and treatment. Before transferring tumor,
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the emitted qi had been used for two weeks to protect tumor from growth in the second and
third groups. After transferring tumor, the emitted qi was given for another two weeks to the
third group to treat tumor. Mice of the three groups were killed at the same time. The change
of the body weight , spleen, thymus, tumor net weight were measured. The result showed the
emitted qi decreased the incidence of tumor. Even though the tumor has developed , persistent
emitted qi could inhibit tumor growth. Short duration of emitted qi treatment had little effect
on body weight
, spleen and thymus , etc. It indicates that the emitted
qi can inhibit tumor
growth directly.
1—47
THE CURATIVVE EFFECT OF THE EMITTED QI ON
MICE WITH M0 4 TUMORS
Qian Shusen,Shen Hongxun
Tumor Lab of Radiation and Nuclear Medicine
, Medical School of Client Univer-
sity ,Beligium
EXPERIMENTS
Experiment
Seventeen male mice were injected intraperitoneally with
1 X 10
5 M0 4 cells
in
0. 2 ml
serumfree culture medium. The mice were divided randomly into two groups
(the treating
group and control group). Since the second day after implantation of M04 cells, the mice of
the treating group were treated every day for 30 minutes by the emitted qi given out through
the hands of qigong doctor Shen Hongxun. The distance between the mice and the hand was
15 to 20 cm.
The control group was not given any treatment. The mice of the two groups were kept
on the same standard pellet diet and tap water ad libitum. The time of death of the mice was
noted down and all the dead mice were autopsied and the volume of the tumor was measured.
Experiment 2
Seventeen female mice were injected intraperitoneally with
1 X 10
5 M0 4 cells in
0. 2 ml
serum culture medium. The mice were divided randomly into the treating group and control
group. On the seventh day after implantation of M0 4 cells the treatment was started and the
curative method and examination were the same as in the 1st experiment.
Experiment 3
Twenty female mice were injected subcutaneously into the right axilla with 1X10
5 M0 4
cells in 0. 2 ml serum-free culture medium and then the mice were divided randomly into the
treating group and control group. On the seventh day after implantation of M0 4 cells, the tu-
mor could be touched and the treatment was started. The curative method and the examina-
tion were the same as in the 1st experiment.
RESULTS
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In experiment
1 and 2
all mice died within 28 days and
all dead mice showed intra-ab-
dominal tumor and haemorrhagic ascites. Most of the tumor nodes and the haemorrhagic as-
cites were collected and their volume was measured individually.
In experiment 3 the mice of the control group died within 34 days, but the mice of the
treating group lived longer. The tumor localized under the skin and some tumor had invaded
into! the pleural cavity. The tumor volume was measured individually according to the formu-
la jv!=a X
2 X 0. 4
. V
is the tumor, a
is the long diameter of the tumor, b
is the perpendicular
shoitt diameter of the tumor.
Through statistical analysis we found that in experiment
1 and 2 the volume of the intra-
abdbminal tumors had
a
significant difference between the treating group and the control
grodp
(in experiment l,p = 0. 0024, in experiment 2,p<C0. 000) but the survival period and
the volumes of the bloody ascites did not have a statistical difference between the treating
group and the control group.
In experiment 3 by statistical comparison, we found that the survival period and the av-^
erage tumor volume per day had
significant difference between the treating group and the
control group (P survival time = 0. 02, p tumor volume = 0. 012), but the end volume of the tui
mor did not have any significant difference
0. 105.
1—48
A RESEARCH ON THE EMITTED QI
; S EFFECT ON THE
IMMUNE STICKING FUNCTION OF RED BLOOD CELLS
TO TUMOR CELLS
Feng Lida, Qian Juqing,Kang Xiaoling
Chinese Immunology Research center
, Beijing
Using tumor red blood cell garlands as an observing index, this paper reports the emittecf
qi's effect on the immune sticking function of mice's red blood cells to tumor cells. We have
found that
, firstly
, mice' s red blood
cells and moxa ascites cancer cells can form tumor
redf
blood cell garlands
j secondly
, the average rate of tumor red blood cell garlands of the mice in-
fluenced by the emitted qi was 31. 68±6. 19, while that of the control mice was 26. 44±1. 43.
The difference was
19. 82%. After we handled the reserch result with the statistic method,
we ^ot P<0. 01
, which proved that the emitted qi helped to promote and increase the immune
sticking function of mice red blood cells to tumor cells. This might imply the emitted qi can|
help red blood cell membrance complement and receptor form garlands after its reaction withl
tumor cells. This
is a new field of tumor immunology which indicates a new direction to the
treatment of tumor. The examination of red blood cell immune activity index has great refer^
ential value to the observation of patients' immune state judgement of qigong treatment ef-
fects; and indication of disease prognosis.
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l
XHE EFFECT OF THE EMITTED QI ON THE CHANGE
OF ANTIBODY DEPENDENCE CELL-MEDIATED CYTO-
TOXICITY (ADCC)OF K CELL OF MICE CAUSED BY IN-
JURY OF THE LEFT AND RIGHT BRAIN CORTEX
Zhou Yong,Yan Xuanzuo
, Zhang Li
Qigong Institute, Beijing College of Traditional Chinese Medicine, Beijing 100029
This paper reports after the injury of left and right brain cortex on the ADCC function of
the K cell of mice
, our observation of the effect of the emitted qi on the change of the ADCC
of the K cell of mice. It was a mechnical
injury. The tissue-section results showed broken
brain cortex of mice, the nerve cell liquifaction necrosis
, phagocyte infiltration
, a light hyper-
plasia of nerve glia cell. It proved that the models were really injured in the brain cortex. The
cortex injury of the left and right brain caused the change of the ADCC activity
; the left brain
cortex injury caused the obvious reduction of the ADCC activity (P<0. 001) 'the right brain
cortex injury caused the increase of the ADCC activity(P<0. 001). It showed that the injury
of the brain cortex really caused the change of the ADCC activity. On this basis,we observed
the adjusting effect of the emitted qi on the change of the ADCC activity. We have found the
emitted
qi has the recovering function on the reduction of the ADCC activity caused by the
left brain cortex injury (P<0. 001) ,and it has the reduction action on the increase of the AD-
CC activity caused by the right brain cortex injury (p<0. 001). These show that the emitted
qi can adjust the imbalanced state of the ADCC activity caused by the left and right brain cor-
tex injury to the balanced state in two ways.
1—50
THE ADJUSTING EFFECT OF THE EMITTED QI ON
THE IMMUNE FUNCTION OF COLD-STRESS MICE
Zhang Li, Wang Li,Yan Yuanzuo,Ge Dongyu,Zhou Yong
Institute of Qigong Science
, Beijing Collge of Traditional Chinese Medicine, Bei-
jing 100029
In this experiment , NIH mice were divided into three groups randomly
(l)the nor
mal control group, (2)the stressed control groupCMice underwent
5 min of HVil’C/day
cold stress exposure
for
8 days), (3) the qigong groups (besides cold-stress
, mice received
emitted qi 30 min/day for 8 days). Then, the index of thymus , spleen and brain were detect-
ed, T cell ,B cells proliferation and the RDCC activity of K cell were used to investigate the
immune adjusting effect of the emitted qi on cold-stressed mice. Our study demonstrated (1)
The T,B cell proliferation rate of the stressed mice (10084±549j5074±499)was obviously
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lower than that of the non-stressed mice (18270 + 1069
; 13704±855)p<0. 01, but the T,B
cell proliferation of the qigong group (23582 ± 1959
; 14601 ± 900) was obviously higher thah
tha^
of the stressed mice group (p<0. 01). (2)The activity of K
cell RDCC of the stressed
mic& (0. 128+ 0. 004)was obviously lower than that of the non-stressed mice(0. 459+0. 007,
p
<0j 001), but the emitted qi can enhance the RDCC activity of the stressed mice(0. 505+ 0.
001>p<0. 001). (3) In cold stress environment
, the immune organs (thymus and spleen)of
mic® were atrophed more significantly (3. 57 ±0. 64,4. 76 ±0. 75) than the non-stressed mice
(4. 28+0. 63;5. 67+ 1. 06,p<0. 01)
, but the emitted qi can alleviate the injury of the thymus
and spleen
(4. 10 + 0. 49,p<0. 01,5. 22 + 1. 06,p>0. 05). (4)The weight of brain of the
stressed mice were lighter (20. 6 + 1. 5)than the non-stressed mice(22. 0+1. 4,p<0. 05), but
the qmitted qi can increase the brain weight of the stressed mice(22. 2 + 1. 5,p<0. 01).
i
1~51
AN OBSERVATION OF THE EFFECT OF THE EMITTED
QI ON THE GROWTH OF MICE
j
Feng Lida
, Chen Shuying
, Zhu Lina
, Zhao Xiuzhen
, Cui Siqi
Chinese Immunology Research Center
, Beijing
, China
.In this experiment
, we have observed the effect of the emitted qi on the growth of mice.
As a comprehensive factor of physical
, chemical and biological functions
, the emitted qi cap
b°th| cure diseases and strengthen health, but can it promote the growth of creatures?In order]
to find out the answer to the question, we are among the first people who do research on this!
subject.
We have observed the growth of over
60 mice and got satisfactory
results. The body
length of the test mice
is averagely 10. 26cm, while that of the control mice
is
9. 86. The dif-
ferepce
is
0. 4cm. The length of the test mice's hind legs is
3. 33cm, while that of the control!
mice: is
3. 23cm. The two results are statistically significant (p<0. 05). Meanwhile, we have!
determined the amount of growth hormone in mice's serum with the isotope method. In the
four experiments, the amounts of the test mice were all higher than those of the control ones]
by 0. 81,2. 3
1. 55 and
1. 43ng/ml respectively. The difference between these two groups was]
very jdistinct (p<0. 01). The result shows
that the emitted
qi can promote the growth of
mice
s bodies and hind legs and help increase the amount
of growth hormone
in mice's:
serurp, which implies that the emitted qi can promote and help the growth of creatures
: and
that offers biological proof to the actual existence of the emitted qi and provides important in-
formation and methods for application and research.
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1—52
THE EFFECT OF THE EMITTED QI TO ACUPOINTS ON
SOMATOSENSORY EVOKED POTENTIAL RECORDED
FROM THE CORTEX FOLLOWING ZUSANLI
( ST36
)
STIMULATION IN CATS
Cui Rongqing
(1) ,Zhao Xiuquan
(1) ,Yang Jiafeng°\Li Hoengmin
C2)
(1)
Department of Physiology
, Beijing College of Traditional Chinese Medicine
(2)
China-Japan Friendship Hospital , Beijing , 100029
, China
The paper has observed the effect of the emitted qi to Zusanli(St36) on the somatosen-
sory evoked potential (SEP)recorded from the cerebral cortex following Zusanli (St 36) stim-
ulation in cats.
The experiments were performed on 21 adult cats
, anesthetized with a single dose of
1 %
chloralose(70mg/kg). Cats were fixed on a stereotaxic table in a shield,room. A general cran-
iotomy were performed over the frontal pole to expose the cortex. Zusanli
(St 36) on both
feet were stimulated by the concentric circles electrodes in order to prevent the diffusion of
the stimulation. A monopolar
, silver ball
(0. 5mm diameter)acted as the recording electrode,
was set on the surface of the cortex to
elicit SEP. The normal control group was measured
before the emitted qi,then asked the qigong master to emit qi to Zusanli (St 36) for about 30
min. and recorded the SEP at the same time. The control group was recorded again 30 min af-
ter cessation of the emission of qi.
Comparison between the emitted qi group and the control group showed that the ampli-
tudes of the SEP increased
in
7 cases (33. 33%)
? decreased
in
12 cases(57. 14%)and un-
changed in 2 cases (9. 53%) ;the latencies were prolonged in 5 cases (23. 81%)
, shortened in 9
cases (42. 86%)and unchanged in 7 cases (33. 33%). The average value of all experimental da-
ta were listed in the table , expressing that the amplitudes of SEP were inhibited by the emit-
ted
qi. There were significant statistical difference in P16 &• P40 waves of them, and the laten-
cies were shortened but no significant change.
Table
:
Effect of the emitted qi to acupoint on somatosensory evoked potential in cats
(meaniSE)
Waves
PI 6
N27
P40
before
(ms).
(/iv)
16. 90±0. 27
9. 50±0. 37
27. 63±0. 55
32. 30±1. 36
41. 04±0. 65
60. 20±2. 73
emitted
(ms)
(juv)
16. 76±0. 34
8. 05±0. 29*
*
26. 70±0. 60
29. 44± 1. 26
39. 71±0, 77
51. 75±2. 63 *
after
(ms)
(Mv)
16. 96±0. 31
8. 78±0. 52
27. 18±0. 59
30. 38 ±1.32
41.00±0. 98
57. 16±3. 76
ms. {latencies
{amplitudes; * P<C0. 05? *
* P<C0. 01 compared with before
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It
is often used one of the methods that activity of evoked potential was recorded from a
special place on the cerebral cortex following one point stimulation on the body to study the
mechanism of the medicine. According to each wave origin of the SEP , primary responses in
20ms are generated in the thalamocortical axons and the primary somatosensory areas of the
ipsilateral cerebral cortex, the second responses in 20— 38ms generated the second smatosen-
sory| areas and the callosal areas, the late responses 38ms generated in the controlateral hemi“
sphere and wide-ranging cerebral cortex. Accordingly the amplitude of the SEP reflects the
area; size of the depolarization and the quantity of excitational neuron and the latency reflects
the yelocity and the distance of the signal to be conducted. The experimental results suggest
thatj the quantity of the nervous activity reduce in the cerebral cortex and the somatosensory
pathway, but the quality and the conduction of their function that take part in the excitation
rise after the animals received the emitted
qi. The phenomena are so much similar to qigong
meditation.
(53
BASIC EXPERIMENTS OF ACTIVATION OF SEED GER-
MINATION AND GROWTH WITH THE EMITTED
QI
AND ANALOGOUS INSTALMENT OF THE EMITTED QI
Nakasato Seiki,Yoshio Machi
Japanese Qigong Institute
Tokyo Denki University , Japan
The basic experiments of activation of seed germination and growth were conducted by
three Japanese qigong masters
(all of the chief instructors of the Japanese Qigong Associa-
tion). In addition, an analogous instalment of the emitted qi was applied to this purpose. The
message of the emitted qi had been recorded on the data recorder and the qi was emitted by!
Japanese qigong masters who cured chronic diseases with
it.
The invited qigong masters emitted their qi towards a certain volume of running water,
turning
it into the
"qi water". Five kinds of seeds were irrigated by the
"qi water"
, running
water
to observe
their germination and growth. Another kind of
"qi water" was prepared
through the analogous instalment of the emitted
qi
, named
the "analogous
qi water". The
analogous instalment of the emitted qi worked directly on the seeds too.
The qigong masters emitted their
qi to the seeds of cabbage, etc. Except one
all seeds
showed good result in budding and growth in comparision with the control group. The bud-
ding and growth increased by 15% compared with the control group (irrigated by the running
watejr radiated by infrared ray).
Up to now, qigong has contributed a lot to keeping
fit and treating diseases. However,
our experiments indicate qi is a source of life and
it is closely related to the nonrandom of life
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