Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 diated by ConA was studied. Mechanisms involved in the cell recognition ,such as cell mem- brane-bound glycoprotein and receptors were also investigated. It was found that the emitted gi treatment reduced the extent of agglutination, and this effect became more obvious with increasing times of treatment. Meanwhile ,the forms of gly- coprotein on cell membranes were also changed ,displayed on a SDS-PAGE developed by the specific Schiff’ s staining method ,among which some bands disappeared and others emerged. This variation may play some role in the process of cell recognition. When the FITC labeled ConA was used as a fluorescent probe to detect ConA “receptor” change in situ with the FCM technique,it was further observed that the amount of ConA “receptor” decreased and its distribution was also changed . These results may partially account for the reversion effect of gigong on SPC-AI cell. 1—42 INHIBITION OF HUMAN NASOPHARYNGEAL CARCI- NOMA CELLS IN VITRO BY THE EMITTED QI AND Y- RAY Cao Qiyuan” ,Li Yongqiong’” ,Cheng Chenging™ ,Liang Jianxiong”” (1) Cancer Institute, Sun Yat-Sen University of Medical Sciences , Guangzhou 510060 (2)Dept. of Radiotherapy , Tumor Hospital,Guangzhou 510060 Using human nasopharyngeal carcinoma cell line (CNE-2) as the target cell, the cells were planted into 96 well plastic microcultured plates ,50 cells per well,one plate as a group. There were 4 groups:the control(c) irradiated by 2Gy Y-ray (R), treated by the emitted qi (Q),and treated by both of them (R+Q). After two days culture,the cell clone(228 cells) under an inverted microscope was observed and counted. The mean value of cell clone (X+S. D. ) in the R+Q group was 9. 2+2. 5,markedly lower than the R group (15. 8+2. 4). There was a marked statistical difference (P<0. 001). The kinetic study showed that the Q group at 48 h. the level of mean value of cell clone(16. 52:2. 2) approached to the R group. After- wards it went up again apparently. However,the R group continuously decreased through 48- 96 h. The emitted qi alone was able to kill or damage the CNE-2 cell in vitro, but this action seemed to be higher reversible than arising by irradiation of Y-ray. 1—43 A PRELIMINARY STUDY OF THE EFFECT OF THE EMITTED QI ON EXPERIMENTAL ANIMALS INFECT- ED BY PNEUMOCYSTIS CARINII Zhang Jinmei, Zhang Ciling ,Chen Fakai Sun Yat-Sen University of Medical Sciences ,Guangzhou 510060 Pneumocystis carinii (P. Carinii) has emerged as the leading opportunistic pathogen and * 103 + Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 a major cause of mortality in AIDS. P. carinii and AIDS have been attracting the interests and attention of many researchers all over the world. Studies of P. carinii by many methods and different ways have important significance. The paper reports for the first time the effect of the emitted qi on P. carinii infected Wistar rats. I, Adult female Wistar rats weighing 150-200g were divided into A(26),B(28),C(26) groups randomly. Group C was the control one. | Two qigong masters emitted their qi to Groups A and B for 15 minutes each time once every other day,7 times altogether. Then P. carinii Pneumonia animal model was built up by a regular method. The rats received a standard treatment regimen of cortison acetate. 25 mg; injected subcutaneously twice a week ,low protein(7%) diet, and tetracycling in rat's drink- ing. water until examination. After drug treatment started, the rats were treated by the emit- ted’ qi for one week . They were examined 4-8 weeks after the drug treatment. | The experimental results suggested that the body weight of the 3 groups decreased , but this was more obvious in the control group. Table 1 shows the difference of the infected rats among the three groups. About the carinii infection rate see Table 1. | For differentiation of infected degree, see Table 2. It shows the difference of the infected degree of P. carinii among the three groups. Table 1. Infected rate of P. carinii group counts PC(+) rate of infection X? test A 26 17 65.4 AtBP>o. 05 B 28 14 50. 0 B:C P<0.01 Cc 26 24 92,3 A+tc P<0. 01 Table 2. Difference of infected degree infection degree group counts of PC(+) heavy moderate light A 17/26 1 9 7 B 14/28 3 1 10 C 24/26 12 10 2 The lung smears from the three groups were stained by Giemsa. It was found that the parasites were the typical P. carinii. Table 3 shows the differences of the sizes of P. carinii cyst, among the three groups. Table 3. Differences of sizes of P. carinii cyst PC from counts diameter (X, ) SE t test A 100 4.78 0. 6518 A+B P>0. 05 B 100 4.98 0. 4980 Bt+C P>0. 05 Cc 100 4.74 0. 5326 AtC P>0.05 “104+ Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 The experimental results suggest that the emitted qi reduces the infection rate and de- gree of Wistar rats by P. carinii. The emitted qi can inhibit the infection of P. carinii. I. Nude mice were divided into two groups . Group A(10) was injected of 5-8 X 10° par- asites by lung innocubation. Group B were injected of parasites which had been treated by the ernitted gi . The parasites were put into a small glass. A qigong master emitted his qi to the parasites 20 minutes and altogether 4 times. Each interval lasted for 48 hrs. The experimental results showed that 10 mice of Group A were infected by PC,in which 4 mice were of moderate infection degree, but in Group B only 6 mice were infected by PC, and all were of light infection degree. Two groups had big difference. 1—44 THE DOUBLE-BLIND TEST OF THE EMITTED QI ON TUMOR FORMATION OF A NASOPHARYNGEAL CAR- CINOMA CELL LINE IN NUDE MICE Chen Xiaojun“” , Yi Qing’ ,Liu Kela™ , Zhang Jinmei® ,Chen Yu Sheng (1)Cancer Center ,Sun Yat-sen University of Medical Sciences (2)Dept. of Physiology ,Sun Yat-sen University of Medical Sciences (3)Qigong Association of Guangdong ,China Following the double-blind method ,we carried out two experiments by using cell culture and nude mice. In the first experiment ,a qigong master treated the poorly differentiated na- sopharyngeal squamous cell carcinoma cell line (CNE-2) with his emitted qi ,followed by the ‘noculation of the treated cell (qigong group) and the untreated cells (control group) into 16 female nude mice (NC-Z strain mice). The results showed that the tumor formation of the treated cells was inhibited by an impressive rate of 100%(3/3). The same procedures -were followed in the second experiment. The results demonstrated that the inhibiting rate was still 100% (4/4). A pathological examination further confirmed that there were no tumor cells in the inoculating site of the gigong group ,whereas poorly differentiated squamous cell carcinomas were observed in tumor of the control group. No tumors were found in the livers and lungs of the mice in the qigong group. But in the control group of the first experiment; tumors metastasized to the lung in mouse 2. No tumors were found in the liver of mice in the first experiment or in the livers and lungs of the other mice . Although the mechanism of the effect of the emitted gi still remains unknown, the results of the present research reveals the necessity as well as the possibility of further investigating the inhibition of tumors and its mechanism. - 105° Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 1-45 AN EXPERIMENTAL RESEARCH OF THE INFLUENCE OF THE EMITTED QI ON CANCER GROWTH, METAS- TASIS AND SURVIVAL TIME OF THE HOST Qian Shusen ,Sun Wei, Liu Qing , WanYi,Shi Xiaodong China Rehabilitaion Research Center » Beijing 100077 ,China In researches of the influence of the emitted qi on cancer growth, metastasis and survival time of host, 114 animal cancer models were used in three batches. Tumor models were formed in mice by transplantation of U27 or MO, cancer cells into subcutaneous tissues of the right armpit respectively. These mice with cancer cells were divided randomly into the qigong group and control group and all mice of the two groups were fed with same food. From the second day or seventh day after transplantation of cancer cells ,the mice of the qigong group were treated for 10~30 minutes with the emitted qi every day, but the mice of the control group were not given any treatment. In experiment 1,on the 20th day after transplantation of , cancer cells ,all mice of the two groups were killed and all tumor volumes were measured j in- dividually. In experiment 2,0n the 23rd and 33rd day after transplantation of cancer cells yall mice of the two groups were killed and all armpit lymph nodes and lungs were taken out indi- vidually and examined histopathologically. In experiment 3,when the mice of the two groups died stheir survival time after transplantation of cancer cells was calculated respectively. The following results were found; 1.In experiment 1,the average tumor volume of the qigong group (31 mice, 2. 25+ 5. 35cm*)was obvioulsy lower than the control group (32mice,6. 32-10. 02cm®) ,p<O. 001. | 2.In experiment 2,the metastatic rate (1/16) of lymph nodes in the qigong group was: significantly lower than the control group(6/15),P<0. 05. 3. In experiment 3, the average survival time(35. 4 d)of mice with cancer in the qigong group (10 mice) was obviously longer than the control group (10 mice , 30. 5d) ,P<0. 002. | These results indicate that the emitted qi can delay cancer growth reduce metastatic rate and prolong the survival time of mice with cancer. 1—46. THE EMITTED QI ON MICE TUMOR PREVENTION AND TREATMENT Feng Lida,Peng Liaomin Chinese Immunology Research Center , Bei jing Through random division, we had 30 mice divided into three groups; the tumor control group( 1 ) qigong protective group( I )and qigong protective and treating group( I )to look into the use of the emitted qi in tumor prevention and treatment. Before transferring tumor, * 106« Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 the emitted qi had been used for two weeks to protect tumor from growth in the second and third groups. After transferring tumor ,the emitted qi was given for another two weeks to the third group to treat tumor. Mice of the three groups were killed at the same time. The change of the body weight, spleen ,thymus,tumor net weight were measured. The result showed the emitted qi decreased the incidence of tumor. Even though the tumor has developed , persistent emitted qi could inhibit tumor growth. Short duration of emitted qi treatment had little effect on body weight,spleen and thymus, etc. It indicates that the emitted qi can inhibit tumor growth directly. 1—47 THE CURATIVVE EFFECT OF THE EMITTED QI ON MICE WITH MO, TUMORS Qian Shusen ,Shen Hongxun Tumor Lab of Radiation and Nuclear Medicine ,Medical School of Chent Univer- sity , Beligium EXPERIMENTS Experiment 1 Seventeen male mice were injected intraperitoneally with 1X 10° MOQ, cells in 0. 2 ml serumfree culture medium. The mice were divided randomly into two groups (the treating group and control group). Since the second day after implantation of MO4 cells,the mice of the treating group were treated every day for 30 minutes by the emitted qi given out through the hands of qigong doctor Shen Hongxun. The distance between the mice and the hand was 15 to 20 cm. The control group was not given any treatment. The mice of the two groups were kept on the same standard pellet diet and tap water ad libitum. The time of death of the mice was noted down and all the dead mice were autopsied and the volume of the tumor was measured. Experiment 2 Seventeen female mice were injected intraperitoneally with 1 10° MO, cells in 0. 2 ml serum culture medium. The mice were divided randomly into the treating group and control group. On the seventh day after implantation of MO, cells the treatment was started and the curative method and examination were the same as in the lst experiment. Experiment 3 Twenty female mice were injected subcutaneously into the right axilla with 1X 10° MO, cells in 0. 2 ml serum-free culture medium and then the mice were divided randomly into the treating group and control group. On the seventh day after implantation of MO, cells,the tu- mor could be touched and the treatment was started. The curative method and the examina- tion were the same as in the lst experiment. RESULTS * 107° Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 In experiment 1 and 2 all mice died within 28 days and all dead mice showed intra-ab- dominal tumor and haemorrhagic ascites. Most of the tumor nodes and the haemorrhagic as- cites were collected and their volume was measured individually. ‘In experiment 3 the mice of the control group died within 34 days, but the mice of the treating group lived longer. The tumor localized under the skin and some tumor had invaded into' the pleural cavity. The tumor volume was measured individually according to the formu- la:v=aXb’X0. 4°. V is the tumor,a is the long diameter of the tumor,b is the perpendicular short diameter of the tumor. ‘Through statistical analysis we found that in experiment 1 and 2 the volume of the intra- abdominal tumors had a significant difference between the treating group and the control group Cin experiment 1,p=0. 0024,in experiment 2,p<(0. 000)but the survival period and the volumes of the bloody ascites did not have a statistical difference between the treating group and the control group. . . In experiment 3 by statistical comparison, we found that the survival period and the av-. erage tumor volume per day had significant difference between the treating group and the control group(P survival time=0. 02,p tumor volume=0. 012), but the end volume of the tu+ mor:did not have any significant difference 0. 105. 1— 48 A RESEARCH ON THE EMITTED QI’ S EFFECT ON THE IMMUNE STICKING FUNCTION OF RED BLOOD CELLS TO TUMOR CELLS Feng Lida,Qian Juging ,Kang Xiaoling Chinese Immunology Research center , Beijing Using tumor red blood cell garlands as an observing index, this paper reports the emitted qi’s effect on the immune sticking function of mice’ s red blood cells to tumor cells. We have found that, firstly ,mice’s red blood cells and moxa ascites cancer cells can form tumor red blood cell garlands ;secondly ,the average rate of tumor red blood cell garlands of the mice in- fluenced by the emitted qi was 31. 6846. 19,while that of the control mice was 26. 44+1. 43. The ‘difference was 19. 82%. After we handled the reserch result with the statistic method, we got P<20. 01,which proved that the emitted qi helped to promote and increase the immune sticking function of mice red blood cells to tumor cells. This might imply the emitted qi can help|red blood cell membrance complement and receptor form garlands after its reaction with tumor cells. This is a new field of tumor immunology which indicates a new direction to the treatment of tumor. The examination of red blood cell immune activity index has great refer- ential value to the observation of patients’ immune state,judgement of qigong treatment ef- fects and indication of disease prognosis. «108 + Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 1—49 THE EFFECT OF THE EMITTED QI ON THE CHANGE OF ANTIBODY DEPENDENCE CELL-MEDIATED CYTO- TOXICITY (ADCC)OF K CELL OF MICE CAUSED BY IN- JURY OF THE LEFT AND RIGHT BRAIN CORTEX Zhou Yong, Yan Xuanzuo,Zhang Li Qigong Institute, Beijing College of Traditional Chinese Medicine, Beijing 100029 This paper reports after the injury of left and right brain cortex on the ADCC function of the K cell of mice, our observation of the effect of the emitted qi on the change of the ADCC of the K cell of mice. It was a mechnical injury. The tissue-section results showed broken brain cortex of mice,the nerve cell liquifaction necrosis ,phagocyte infiltration ,a light hyper- plasia of nerve glia cell. It proved that the models were really injured in the brain cortex. The cortex injury of the left and right brain caused the change of the ADCC activity ;the left brain cortex injury caused the obvious reduction of the ADCC activity (P<0. 001) ;the right brain cortex injury caused the increase of the ADCC activity (P<0. 001). It showed that the injury of the brain cortex really caused the change of the ADCC activity. On this basis ,we observed the adjusting effect of the emitted qi on the change of the ADCC activity. We have found the emitted gi has the recovering function on the reduction of the ADCC activity caused by the left brain cortex injury (P<0. 001) ,and it has the reduction action on the increase of the AD- CC activity caused by the right brain cortex injury (p<0. 001). These show that the emitted qi can adjust the imbalanced state of the ADCC activity caused by the left and right brain cor- tex injury to the balanced state in two ways. 1—50 THE ADJUSTING EFFECT OF THE EMITTED QI ON THE IMMUNE FUNCTION OF COLD-STRESS MICE Zhang Li, Wang Li, Yan Yuanzuo,Ge Dongyu Zhou Yong Institute of Qigong Science , Beijing Collge of Traditional Chinese Medicine , Bei- jing 100029 In this experiment ,NIH mice were divided into three groups randomly—— (1)the nor- mal control group, (2)the stressed control group (Mice underwent 5 min of 14°C +1°C/day cold stress exposure for 8 days), (3) the qigong groups (besides cold-stress, mice received emitted qi 30 min/day for 8 days). Then, the index of thymus,spleen and brain were detect- ed,T cell,B cells proliferation and the RDCC activity of K cell were used to investigate the immune adjusting effect of the emitted qi on cold-stressed mice. Our study demonstrated (1) The T.B cell proliferation rate of the stressed mice (10084-+549;5074+499) was obviously «109» Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 lower than that of the non-stressed mice (18270+ 1069; 13704+855)p<O. 01, but the T,B cell proliferation of the qigong group (23582+1959;14601-+ 900) was obviously higher than that of the stressed mice group (p<0. 01). (2) The activity of K cell RDCC of the stressed micé (0. 128-+0. 004) was obviously lower than that of the non-stressed mice (0. 459-40. 007,p <0. 001) ,,but the emitted gi can enhance the RDCC activity of the stressed mice (0. 505-0, 001,p<0. 001). (3)In cold stress environment, the immune organs (thymus and spleen) of mice were atrophed more significantly (3. 57 +0. 64;4. 760. 75)than the non-stressed mice (4, 28-0. 6335. 6741. 06,p<0. 01), but the emitted qi can alleviate the i injury of the thymus andispleen (4. 10+0. 49,p<0. 0135. 22+1. 06, p> 0. 05). (4) The weight of brain of the stressed mice were lighter (20. 6+1. 5)than the non-stressed mice(22. O+1. 4,p<0. 05), but the emitted qi can increase the brain weight of the stressed mice(22. 2+1.5,p<0. 01). 1—51 AN OBSERVATION OF THE EFFECT OF THE EMITTED | QI ON THE GROWTH OF MICE ! Feng Lida,Chen Shuying, Zhu Lina, Zhao Xiuzhen, Cui Sigi Chinese Immunology Research Center , Beijing , China In this experiment ,we have observed the effect of the emitted qi on the growth of mice. As a comprehensive factor of physical,chemical and biological functions, the emitted qi can) both cure diseases and strengthen health, but can it promote the growth of creatures?In order to find out the answer to the question, we are among the first people who do research on this) subject. We have observed the growth of over 60 mice and got satisfactory results. The body: length of the test mice is averagely 10. 26cm, while that of the control mice is 9. 86. The dif- ference i is 0. 4cm. The length of the test mice’s hind legs is 3. 33cm,while that of the control mice is 3. 23cm. The two results are statistically significant (p<0. 05). Meanwhile, we have determined the amount of growth hormone in mice’s serum with the isotope method. In the four | experiments »the amounts of the test mice were all higher than those of the control ones. by 0.81,2.3 1.55 and 1. 43ng/ml respectively. The difference between these two groups was very distinct (p<0. 01). The result shows that the emitted qi can promote the growth of. mice's bodies and hind legs and help increase the amount of growth hormone in mice’ s' serum , which implies that the emitted qi can promote and help the growth of creatures; rand | that offers biological proof to the actual existence of the emitted qi and provides important in- formation and methods for application and research. - 110+ Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 1—52 THE EFFECT OF THE EMITTED QI TO ACUPOINTS ON SOMATOSENSORY EVOKED POTENTIAL RECORDED FROM THE CORTEX FOLLOWING ZUSANLI (ST36 ) STIMULATION IN CATS Cui Rongqing” , Zhao Xiuquan™ , Yang Jiafeng ,Li Hoengmin™ (1)Department of Physiology , Beijing College of Traditional Chinese Medicine (2)China-Japan Friendship Hospital , Beijing , 100029 ,China The paper has observed the effect of the emitted qi to Zusanli(St36) on the somatosen- sory evoked potential (SEP)recorded from the cerebral cortex following Zusanli (St 36) stim- ulation in cats. The experiments were performed on 21 adult cats,anesthetized with a single dose of 1% chloralose(70mg/kg). Cats were fixed on a stereotaxic table in a shield room. A general cran- iotomy were performed over the frontal pole to expose the cortex. Zusanli (St 36) on both feet were stimulated by the concentric circles electrodes in order to prevent the diffusion of the stimulation. A monopolar, silver ball (0. 5mm diameter)acted as the recording electrode, was set on the surface of the cortex to elicit SEP. The normal control group was measured before the emitted gi,then asked the qigong master to emit qi to Zusanli (St 36) for about 30 min. and recorded the SEP at the same time. The control group was recorded again 30 min af- ter cessation of the emission of qi. Comparison between the emitted qi group and the control group showed that the ampli- tudes of the SEP increased in 7 cases (33. 33%) decreased in 12 cases (57. 14%) and un- changed in 2 cases(9. 53%) ;the latencies were prolonged in 5 cases(23. 81%), shortened in 9 cases (42. 86% and unchanged in 7 cases (33. 33%). The average value of all experimental da- ta were listed in the table expressing that the amplitudes of SEP were inhibited by the emit- ted qi. There were significant statistical difference in P16 & P40 waves of them,and the laten- cies were shortened but no significant change. Table; Effect of the emitted qi to acupoint on somatosensory evoked potential in cats (mean +SE) Waves Pi6 N27 P40 before (ms). 16. 90-0. 27 27. 63-£0. 55 41.0440. 65 Cuv) 9.5040. 37 32. 30+1. 36 60. 2042. 73 emitted (ms) 16. 7640. 34 26. 70-0. 60 39. 7140. 77 (uv) 8.0520. 29 * * 29. 44-1. 26 51. 7542, 63 * after (ms) 16. 96-0. 31 27,18+0. 59 41. 000. 98 (uv) 8.7840. 52 30. 38-b 1. 32 57.1643. 76 ms. :latencics;pv. :amplitudes; x P<0.05, * * P<.0. 01 compared with before ‘lll: Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
‘Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001 -6 _It is often used one of the methods that activity of evoked potential was recorded from a special place on the cerebral cortex following one point stimulation on the body to study the mechanism of the medicine. According to each wave origin of the SEP, primary responses in Z20ms are generated in the thalamocortical axons and the primary somatosensory areas of the ipsilateral cerebral cortex,the second responses in 20—38ms generated the second smatosen- sory areas and the callosal areas, the late responses 38ms generated in the controlateral hemi- sphere and wide-ranging cerebral cortex. Accordingly the amplitude of the SEP reflects the area size of the depolarization and the quantity of excitational neuron and the latency reflects the velocity and the distance of the signal to be conducted. The experimental results suggest that the quantity of the nervous activity reduce in the cerebral cortex and the somatosensory pathway, but the quality and the conduction of their function that take part in the excitation rise after the animals received the emitted qi. The phenomena are so much similar to qigong meditation. 1—533 BASIC EXPERIMENTS OF ACTIVATION OF SEED GER- ! MINATION AND GROWTH WITH THE EMITTED QI AND ANALOGOUS INSTALMENT OF THE EMITTED QI Nakasato Seiki, Yoshio Machi Japanese Qigong Institute Tokyo Denki University , Japan The basic experiments of activation of seed germination and growth were conducted by’ three Japanese qigong masters (all of the chief instructors of the Japanese Qigong Associa- tion). In addition,an analogous instalment of the emitted gi was applied to this purpose. The message of the emitted qi had been recorded on the data recorder and the qi was emitted by Japanese gigong masters who cured chronic diseases with it. The invited qigong masters emitted their qi towards a certain volume of running water 5 turning it into the “qi water”. Five kinds of seeds were irrigated by the “ qi water” running water to observe their germination and growth. Another kind of “qi water” was prepared! through the analogous instalment of the emitted qi,named the “analogous qi water”. The! analogous instalment of the emitted qi worked directly on the seeds too. | The qigong masters emitted their qi to the seeds of cabbage, etc. Except one all seeds showed good result in budding and growth in comparision with the control group. The bud- ding and growth increased by 15% compared with the control group Cirrigated by the running water radiated by infrared ray). Up to now, qigong has contributed a lot to keeping fit and treating diseases. However». our experiments indicate qi is a source of life and it is closely related to the nonrandom of life *112- Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 process. Further more,qi as a life message activates the life process of creatures (human be- ing,animal,plant and microorganism). 1—54 THE IR STUDY OF HEAVY WATER TREATED BY THE EMITTED QI Ye Fangyang,Chen Tianyou, Zhang Wengong Qigong Clinic,Fuzhou Hospital of Traditional Chinese Medicine ,Fuzhou,China We applied the infrared spectroscope in the research of the effect of the emitted qi on heavy water with the running water as the control. The results showed that under the effect of the emitted gi,the frequency of the stretch absorption band of O-D of ordinary water did not change, while the absorption intensity decreased;in the meantime, the frequency of the stretech absorption band O-H of ordinary water did not change either ,but its absorption in- tensity increased. The research not only discovers the effect of the emitted gi on the IR of heavy water and running water are definitely the opposite, but also proves that the changes of IR are related to the action way of the emitted qi. The rigorious experiments have proved the effect of the emitted qi on heavy water and running water is objectively existing. According to the above results,a preliminary explanation is provided. It is about the effect of the emitted qi on the nonrandom structure of molecules of heavy water and running water. 1—55 THE MATERIAL EFFECT OF MIND AND QI—— A RE- SEARCH WITH THE HELP OF THE LASER LAMAN SPECTRUM ANALYSER Chen Guoguang Cardre Training College, Zhaotong Area, Yunnan Province In order to research the effect of gigong I emitted my qi to water and tested it with an SPEx 1403 Laser Laman Spectrum Analyser,to analyse the structure of water molecule and its vibration. To exclude the influence possibly resulted from distance difference ,impurties in water and other elements, distilled water was chosen to be the sample. The background spec- taum was obtained in every test. In addition to this,the following experiments were conduct- ed. (1)Heating the sample water to 48°C. (2) Applying strong magnetics to the sample water for 20 minutes. (3) Analogous emission of qi. It was found that the result provided by the Laman Spectrum to the sample water remained unchanged. It can thus conclude that the hu- man body temperature,the magnetic field generated by electronic appliances and the analo- gous emission of qi have no effect when it is analysed by the Laman spectrum. However ,I emitted my qi at a distance of 20cem,over 4m,4km(or kkm)to the sample wa- ter changes on the Laman specturm were seen. It proved that a kind of special energy is re- * 113° Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 leased when qigong was being exercised and that the energy existing in human bodies, be- sides ,the peak of the spectrum varies in different qigong patterns, levels and distance. At the same distance ,different reults reflected by the spectrum showed that different amount of en- ergy could be generated with different forms of qi emission. The energy produced from the acupoints on meridians is greater. Qi emitted by mind and at a long distance was known as a specific qigong pattern. The change of intensity of the peak of the Laman was greater in com- parision of the qigong state. It reveals that only 3—7% cerebral cells are used in normal con- dition but under the specific state, the potentials are brought about. Hypothalamus is one of the key nerve centres of the body. The research of the nervous center of systerma nervorum automaticum in cerebrum depends on the prolonging or shortening of the vestibule time val- ue. The brain will be equipped with high energy derived from the retrograding effect pro- duced by mind. The key of my emisson of qi at a long distance or by mind, lies in the master- ing of the knowledge of eight diagrams ,integration of man and universe. Water takes up 65% of the human body. Molecules of water take part in the physiologi- cal ,biochemical activities. The variation on the Laman spectrum shows the water’s response | to qigong. Treating patients with the emitted qi and let them drink the “qi” water is to pro- duce the radiating effect of the qigong master. Psychology is the feedback of mind. It will sprial up from physiology to R. Spreeg’s psychology. The feedback energy can be used to di- rect further treatment of patients and be applied to agricultural production and scientific ex- periments. 1— 56 THE EFFECT OF THE EMITTED QI ON THE CHEMICAL SHIFT OF ACTIVE PROTON Zhao Jing, Zhan Diankun, Zhao Xiaomei, Yang Guisheng Nankai University, Tianjin, Beijing College of Acupuncture ,Moxibustion Traumo- tology, Tianjin Society of Somatic Science In the paper, we observed the effect of the emitted qi on the spectrum of the alcohol and - ethanoic acid by HNMR in order to discover the chemical basis of the emitted gi effect. Thirty experiments showed the gi emitted by strong qigong masters could shift the spec- trum peak of active protons but did not effect other peaks. The distance of the peak shift and the continued time depended on the power of the qigong master and the time of the accepting qi. The direction of the peak shift could be changed by the will of the qigong master. In some examples »there were swings in the variation of the peak shift. When a qigong master forms a field, there were also the same variations. Common men and weaker power qigong master could not effect the examples even if the emitted qi lasted for a long time. The peak shift of the active protons could be considered the variation of the H-bond number in the example solution. The peak shift towards higher field range (right shift) field ‘lids Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 (left shift)showed the decrease of the H-bond,otherwise the increase of the H-bonds. If the decrease of the H-bonds was considered the bonds being destroyed by qi ,then the increase of the H-bonds should be considered the emitted qi having absorbed partial energy from the so- lution. This is impossible. So,the abovementioned result cannot be explained by present theo- ries. However, it is sure that the emitted qi effect is objective reality. The variance of the physiology and biochemistry of microorganism, plants and animals and the variances of the structure in biological molecules of all kinds depend on the H-bonds being destroyed and re- aligned intimately. The reason of the swing in the peak position of the active H waits to be explored further. 1—57 THE EFFECT OF THE EMITTED QI ON THE HIGHER- TEMPERATURE-SUPERCONDUCTIVE MATERIALS Huo Yuhua, Zhao Jing , Zhao Shouzhen, Zhao Xiaomei, Liu Shuifa Nankai University , Beijing College of Acupuncture, Moxibustion, Traumotology Tianjin Society of Somatic Science Some experiments have showed that the emitted qi could produce biological and chemical ‘effects, change the property of different kinds of solution. In HNMR experiments, we have showed that the emitted qi effect on the variation of biological molecular structure is due to the H-bonds. Can the emitted gi effect the solid materials with very steady structure? For this, we have conducted many experiments. In this experiment ,a few qigong masters emitted qi to the Y-, Te-and Bi-system’s polycrystal-superconductive materials. Then, we measured Te and Ic of these materials. We found that some qigong masters could make Tc and Ic down,others could make them up,and the changes were quite steady. After changing from room-temperature to liquid-nitrogen temperature for many times,the“un-accepting” qi “ma- terials went bad in their property. When a qigong master emitted his qi to materials, the Ic and Tc of these materials could not only return to the original values, but be made to exceed their original values. That is,the emitted gi can return and improve the property of the high- er-temperature-superconductive materials. In this experiment,it has also been found that when a qigong master formed a field, the Te and Ic of the higher-temperature-materials being positioned in the field should change in two-directions. This is in agreement with our other experiments about other biological and chemical materials. At present ,we are researching the influence of the emitted qi on the property of higher- temperature-superconductive devices. In view of the devices having been put in use, it is ex- pected that this research will have a fairly economical value. The physical mechanism that the emitted qi can return or improve the property of super- conductive materials (and devices)are being under investigation. * 115° Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6
Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6 1— 58 A STUDY OF THE HYPERCHROMIC EFFECT AND HYPOCHROMIC EFFECT OF THE DNA UNDER THE EFFECT OF THE EMITTED QI AND THE WILL Li Luying ,Xu Hongwei,Li Xiaohui, Wen Qinfen, Li Jiamei, Li Shenzhuang Research Society of Human Body ,Qigong Medical College Measurements of 260nm absorbances for. bovine thymus DNA and yeast RNA under the action of the emitted qi and will of qigong masters were conducted. The result showed as fol- lows. DNA samples which were treated by different qigong masters had different hyper- chromic effects (P<0. 01) which could be further followed and observed in a refrigerator for 4 ~~A8 hours. There was no change for RNA. It appears to show that the emitted qi and the will of qigong masters may have effect on DNA conformation. 1—59 THE EFFECT OF THE EMITTED QI ON THE REACTION OF MALATE DEHYDROGENASE Zhang Fengde, Zhao Jing , Yue Huigqin, Liu Guiqin, Zhao Xiaomei Department of Biology, Nankai University, Tianjin Beijing College of Acupunc- ture Moxibustion , Traumotology Malate Dehydrogenase (MDH) ,an important oxidoreductase in various tissues ,is one of the indepensable enzymes in the normal metabolism of organisms. A study has been made to show the change of MDH reaction in a few kinds of tissues after the effect of the emitted qi on rabbits, 18 rabbits were divided into 3 groups. One out of six was the control one. The other 5 received the emitted qi. 7 kinds of tissues from the liver, skeletal muscle, spleen, heart ,adrenal gland,kidney ,lungs of the samples were analyzed after they were killed. Ultra- violet spectrophotometry was used to measure the protein concentration of the tissue extract, and then the MDH activity. It was found the emitted gi worked on skeletal, muscle, lungs, heart and spleen obviously in two-directions. However for the liver, kidney and adrenal gland, the emitted gi had somewhat inhibitory effect on MDH. The result reveals that the emitted qi is not capable of having effect on every individual,its effective level is 52%. The emitted qi is most effective on the heart and skeletal muscle among the tissues tested. ° 116° Approved For Release 2000/08/15 : CIA-RDP96-00792R000400430001-6