dataesr/scientific-paragraphs-categorization huggingface dataset:
Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: don. cowan@up. ac. za Gillian Maggs-Kölling, Gobabeb-Namib Research Institute, Walvis Bay, Namibia; e-mail: gillian@gobabeb. org 1; Identities = 448 /471 (95 %), six gaps (1 %)), Phaeothecoidea melaleuca (strain CPC 17223, GenBank HQ599594. 1; Identities = 509/543 (94 %), nine gaps (1 %)) and Camarosporula persooniae (strain CBS 116258, GenBank JF770449. 1; Identities = 513/549 (93 %), 13 gaps (2 %)). Closest hits using the LSU sequence are Lapidomyces hispanicus (strain CBS 118764, GenBank KF310016. 1; Identi-ties = 759 /764 (99 %), no gaps), Phaeothecoidea melaleuca (strain CPC 17223, GenBank HQ599595. 1; Identities = 827/843 (98 %), two gaps (0 %)), Xenoconiothyrium catenata (strain CMW 22113, GenBank JN712570.
1; Identities = 831/849 (98 %), two gaps (0 %)) and Camarosporula persooniae (strain CBS 116258, GenBank JF770461. 1; Identities = 829 /849 (98 %), two gaps (0 %)). Distant hits obtained using the rpb2 sequence had highest similarity to Capnodium salicinum (strain CBS 131. 34, GenBank KT216553. 1; Identities = 196/255 (77 %), 12 gaps (4 %)), Lecanosticta jani (strain Guat. 267. 44. N1, GenBank MK015342. 1; Identities = 211/280 (75 %), 11 gaps (3 %)) and Lecanosticta brevispora (strain CMW46503, Gen-Bank MK015309. 1; Identities = 189/248 (76 %), nine gaps (3 %)). Closest hits using the tub2 sequence had highest similarity to Lapidomyces hispanicus (strain TRN500, GenBank KF546778. 1; Identities = 315/383 (82 %), five gaps (1 %)), Petrophila incerta (strain TRN139b, GenBank KF546769. 1; Identities = 305/372 (82 %), 16 gaps (4 %)) and Cladosporium sphaerospermum (strain 18ESMA010, GenBank MT881938.
Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: don. cowan@up. ac. za Gillian Maggs-Kölling, Gobabeb-Namib Research Institute, Walvis Bay, Namibia; e-mail: gillian@gobabeb. org 1; Identities = 449 /480 (94 %), 11 gaps (2 %)), Rhytidhysteron rufulum (strain CFE-147, GenBank MN653261. 1; Identities = 441/471 (94 %), 12 gaps (2 %)) and Gloniopsis leucaenae (strain MFLUCC 17-2425, GenBank NR_163334. 1; Identities = 426/456 (93 %), 11 gaps (2 %)). Closest hits using the LSU sequence are Hysterodifractum partisporum (voucher HUEFS 42865, GenBank NG_060652. 1; Identities = 884/907 (97 %), two gaps (0 %)), Hysterobrevium mori (strain GKM 1214, GenBank GQ221895. 1; Identities = 880/906 (97 %), one gap (0 %)) and Gloniopsis subrugosa (strain SMH557, GenBank GQ221896.
1; Identities = 879/906 (97 %), one gap (0 %)). Distant hits obtained using the tef1 sequence had highest similarity to Gloniopsis subrugosa (strain GKM 1214, GenBank GU397336. 1; Identities = 401/499 (80 %), 22 gaps (4 %)) and Hysterobrevium mori (strain GKM 1013, GenBank GU397338. 1; Identities = 402/500 (80 %), 31 gaps (6 %)). Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P. O. Box 85167, Colour illustrations. Flamingos at Walvis Bay lagoon. Conidiomata on OA; conidiogenous cells giving rise to conidia; conidia. Scale bars = 10 µm. 3508 AD Utrecht, The Netherlands; e-mail: p. crous@wi. knaw. nl & e. groenewald@wi. knaw. nl Neriman Yilmaz, Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: neriman.
yilmazvisagie@fabi. up. ac. za Don Cowan, Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Notes -Deniquelata was established for a genus of Dothi-deomycetes with pseudothecia giving rise to bitunicate asci with pseudoparaphyses and brown, muriform ascospores (Ari-yawansa et al. 2013). Deniquelata hypolithi is the first asexual morph linked to a species of Deniquelata. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had high-est similarity to Deniquelata barringtoniae (strain MFLUCC 11-0422, GenBank NR_111779. 1; Identities = 669/691 (97 %), 13 gaps (1 %)), Deniquelata quercina (strain 1050-SAB SA5 1, GenBank MT820404. 1; Identities = 649/681 (95 %), 14 gaps (2 %)) and Didymocrea leucaenae (strain MFLUCC 17-0896, GenBank NR_164298.
1; Identities = 477/523 (91 %), 14 gaps (2 %)). Closest hits using the LSU sequence are Deniquelata quercina (strain 1050-SAB SA5 1, GenBank MT808605. 1; Identities = 789 /799 (99 %), one gap (0 %)), Deniquelata bar-ringtoniae (strain MFLUCC 16-0271, GenBank MH260291. 1; Identities = 805/816 (99 %), four gaps (0 %)) and Neokalmusia thailandica (strain MFLUCC 16-0399, GenBank KY706131. 1; Identities = 778 /814 (96 %), three gaps (0 %)). Distant hits obtained using the actA sequence had highest similarity to Parastagonospora nodorum (strain LDN03-Sn4, GenBank CP022803. 1; Identities = 457/505 (90 %), five gaps (0 %)), Se-tosphaeria rostrata (strain BRIP 11426, GenBank LT837589. 1; Identities = 474/534 (89 %), 15 gaps (2 %)) and Setosphaeria minor (strain BRIP 14612, GenBank LT837609. 1; Identities = 471/531 (89 %), nine gaps (1 %)).
Closest hits using the rpb2 sequence had highest similarity to Deniquelata vittalii (strain PUFD39, GenBank MF168942. 1; Identities = 744 /823 (90 %), no gaps), Didymocrea leucaenae (strain MFLUCC 17-0896, GenBank MK434905. 1; Identities = 733 /823 (89 %), no gaps) and Pseudopithomyces karoo (strain MUCL 9365, Gen-Bank LK936424. 1; Identities = 766/905 (85 %), four gaps (0 %)). Closest hits using the tef1 sequence had highest similarity to Pseudopithomyces entadae (strain MFLUCC 17-0917, GenBank MK360083. 1; Identities = 889/920 (97 %), no gaps), Tremateia murispora (strain GZCC 18-2787, GenBank MK986482. 1; Identities = 902 /934 (97 %), no gaps) and Tre-mateia chromolaenae (strain MFLUCC 17-1425, GenBank MT235778. 1; Identities = 882/915 (96 %), two gaps (0 %)). The tef1 sequences of CPC 38988 and 38984 differ by a single substitution (935 /936 (99 %)).
Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: don. cowan@up. ac. za Gillian Maggs-Kölling, Gobabeb-Namib Research Institute, Walvis Bay, Namibia; e-mail: gillian@gobabeb. org Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: don. cowan@up. ac. za Gillian Maggs-Kölling, Gobabeb-Namib Research Institute, Walvis Bay, Namibia; e-mail: gillian@gobabeb. org Etymology. Name refers to Walvis Bay, Namibia, where it was collected. 1; Identities = 544/551 (99 %), five gaps (0 %)), Phialocephala fluminis (strain CBS 351. 85, GenBank MH861889.
1; Identities = 594/619 (96 %), nine gaps (1 %)) and Knufia perfecta (strain IRAN 2553C, GenBank MF062036. 1; Identities = 519/562 (92 %), 21 gaps (3 %)). The ITS sequences of CPC 38983, 38985 and 38987 are identical (616/616 nucleo-tides). Closest hits using the LSU sequence are Phialocephala fluminis (strain CBS 351. 85, GenBank MH873578. 1; Identities = 845/855 (99 %), two gaps (0 %)), Knufia perforans (strain CBS 885. 95, GenBank NG_042586. 1; Identities = 827/ 845 (98 %), two gaps (0 %)) and Knufia sp. MV-2018 (strain MLT-8, GenBank MT636937. 1; Identities = 817/ 836 (98 %), two gaps (0 %)). The LSU sequences of CPC 38983, 38985 and 38987 are identical (814/814 nucleotides). Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P. O. Box 85167, Colour illustrations.
Culture characteristics -Colonies erumpent, spreading, with sparse aerial mycelium and smooth, lobate margin, reach-ing 8 mm diam after 2 wk at 25 °C. On MEA surface and reverse umber; on PDA surface and reverse olivaceous grey; on OA surface umber. Typus. Namibia, Walvis Bay, from hypolith under a quartz stone, 19 Nov. 2019, P. W. Crous, HPC 3101 (holotype CBS H-24501, culture ex-type CPC 38988 = CBS 146989, ITS, LSU, rpb1, tef1 (second part) and tub2 sequences GenBank MZ064433. 1, MZ064490. 1, MZ078188. 1, MZ078251. 1 and MZ078265. 1, MycoBank MB 839520). Additional material examined. Namibia, Walvis Bay, from hypolith under a quartz stone, 19 Nov. 2019, P. W. Crous, HPC 3101, culture CPC 38984 = CBS 146990, ITS, LSU, rpb1, tef1 (second part) and tub2 sequences GenBank MZ064434. 1, MZ064491.
1, MZ078189. 1, MZ078252. 1 and MZ078266. 1. (from marble stone, Vatican City; endoconidia 4. 4-5. 0 μm diam; Isola et al. 2016), from which it is phylogenetically and mor-phologically distinct. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence of CPC 38988 had highest similarity to Knufia marmoricola (strain PiN-A2, GenBank KY887804. 1; Identities = 483/505 (96 %), nine gaps (1 %)), Knufia petricola (strain 2Y43B, GenBank MT126632. 1; Identities = 464/486 (95 %), ten gaps (2 %)) and Knufia per-forans (strain CBS 885. 95, GenBank NR_121502. 1; Identities = 569/596 (95 %), nine gaps (1 %)). The ITS sequences of CPC 38988 and 38984 differ by an extra thymine nucleotide at two alignment positions (624/626 (99 %)). Closest hits using the LSU sequence of CPC 38988 are Knufia sp.
MV-2018 (strain MLT-8, GenBank MT636937. 1; Identities = 850/852 (99 %), no gaps), Knufia marmoricola (strain CCFEE 5716, GenBank KR781074. 1; Identities = 855/858 (99 %), no gaps) and Knufia perforans (strain CBS 885. 95, GenBank NG_042586. 1; Identi-ties = 855/858 (99 %), no gaps). The LSU sequences of CPC 38988 and 38984 are identical (796/796 (100 %)). Closest hits using the rpb1 sequence of CPC 38988 had highest similarity to Knufia sp. LS-2015b (strain CGMCC 3. 17226, GenBank KP226509. 1; Identities = 565/593 (95 %), no gaps), Knufia petricola (strain CBS 726. 95, GenBank KC978743. 1; Identities = 575/631 (91 %), no gaps) and Glyphium elatum (strain CBS 268. 34, GenBank FJ941902. 1; Identities = 479/518 (92 %), no gaps). The rpb1 sequences of CPC 38988 and 38984 are identi-cal (682 /682 (100 %)).
Closest hits using the tef1 sequence of CPC 38988 had highest similarity to Knufia sp. LS-2015b (strain CGMCC 3. 17301, GenBank KP175012. 1; Identities = 881/917 (96 %), no gaps), Knufia petricola (strain A95, GenBank MT859426. 1; Identities = 895 /953 (94 %), no gaps) and Knufia epidermidis (strain CGMCC 3. 17300, GenBank KP175014. 1; Identities = 837/918 (91 %), two gaps (0 %)). Dis-tant hits obtained using the tub2 sequence of CPC 38988 had highest similarity to Phialophora americana (strain CBS 102234, GenBank KU306351. 1; Identities = 207/ 253 (82 %), nine gaps (3 %)), Knufia peltigerae (strain CGMCC 3. 17283, GenBank KP226562. 1; Identities = 173/204 (85 %), two gaps (0 %)) and 'Anthracinomyces ramosus' (strain CGMCC 3. 16367, GenBank KP226556. 1; Identities = 175/207 (85 %), three gaps (1 %)).
Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: don. cowan@up. ac. za Gillian Maggs-Kölling, Gobabeb-Namib Research Institute, Walvis Bay, Namibia; e-mail: gillian@gobabeb. org 1; Identities = 569/573 (99 %), one gap (0 %)), Asordaria humana (strain CBS 416. 82, GenBank MH861509. 1; Identities = 569/573 (99 %), one gap (0 %)) and Sordaria macro-spora (strain CBS 346. 62, GenBank MH858175. 1; Identities = 569 /573 (99 %), one gap (0 %)). Closest hits using the LSU sequence are Sordaria lappae (strain CBS 154. 97, GenBank MH874251. 1; Identities = 807/808 (99 %), no gaps), Asordaria humana (strain CBS 416. 82, GenBank MH873255. 1; Identities = 807/808 (99 %), no gaps) and Sordaria islandica (strain CBS 512.
77, GenBank MH872859. 1; Identities = 807/808 (99 %), no gaps). Closest hits using the actA sequence had highest similarity to Sordaria macrospora (strain k-hell, GenBank XM_024655553. 1; Identities = 398/405 (98 %), no gaps), Neurospora crassa (strain OR74A, GenBank XM_011396324. 1; Identities = 394/405 (97 %), no gaps) and Neurospora tetra-sperma (strain FGSC 2508, GenBank XM_009849372. 1; Identities = 393/405 (97 %), no gaps). Closest hits using the rpb2 sequence had highest similarity to Sordaria fimicola (strain CBS 723. 96, GenBank DQ368647. 1; Identities = 844/882 (96 %), no gaps), Neurospora pannonica (strain TRTC51327, GenBank AY780185. 1; Identities = 863/902 (96 %), no gaps) and Sordaria clematidis (voucher MFLU 16-2138, GenBank MT394717. 1; Identities = 803/842 (95 %), no gaps).
Closest hits using the tef1 sequence had highest similarity to Pseudo-neurospora sp. (strain FMR 12156, GenBank HG326875. 1; Identities = 447/494 (90 %), 15 gaps (3 %)), Pseudoneurospora amorphoporcata (strain CBS 626. 80, GenBank FR774344. 1; Identities = 440/487 (90 %), 16 gaps (3 %)) and Neurospora pannonica (strain FGSC 7221, GenBank FR774377. 1; Identi-ties = 425/483 (88 %), 17 gaps (3 %)). Closest hits using the tub2 sequence had highest similarity to Asordaria tenerifae (strain CBS 264. 86, GenBank AY681206. 1; Identities = 447/480 (93 %), three gaps (0 %)), Pseudoneurospora amorphopor-cata (strain CBS 626. 80, GenBank FR774294. 1; Identities = 436 / 469 (93 %), seven gaps (1 %)) and Sordaria lappae (strain CBS 154. 97, GenBank AY681205. 1; Identities = 448/482 (93 %), eight gaps (1 %)).
Phaeosphaeriopsis sansevieriae & Lasionectria sansevieriae Fungal Planet 1209 & 1210 -13 July 2021 Phaeosphaeriopsis sansevieriae Crous, sp. nov. Etymology. Name refers to the host genus Sansevieria from which it was Typus. South africa, Mpumalanga, Mbombela, Buffelskloof Nature isolated. Reserve, on leaves of Sansevieria hyacinthoides (Ruscaceae), 23 Nov. Classification -Phaeosphaeriaceae, Pleosporales, Dothi-deomycetes. 2018, P. W. Crous, HPC 3143 (holotype CBS H-24496, culture ex-type CPC 38956 = CBS 146984, ITS, LSU and rpb2 sequences GenBank MZ064438. 1, MZ064495. 1 and MZ078204. 1, MycoBank MB 839524). Conidiomata red-brown, globose, 80 -300 µm diam, with cen-tral ostiole (40 -60 µm diam), frequently densely aggregated; wall of 6 -8 layers of brown textura angularis. Conidiophores reduced to conidiogenous cells lining inner cavity, hyaline, Additional material examined.
South africa, Mpumalanga, Mbombela, Buffelskloof Nature Reserve, on leaves of Sansevieria hyacinthoides (Rusca-ceae), 23 Nov. 2018, P. W. Crous, HPC 3143 (CBS H-24540, culture CPC 39087 = CBS 147076, ITS, LSU and rpb2 sequences GenBank MZ064439. 1, MZ064496. 1 and MZ078205. 1). smooth, ampulliform, phialidic, 5-7 × 5-6 µm. Conidia solitary, aseptate, golden brown, verruculose, ellipsoid to subcylindrical with obtuse ends, (5 -)6(-7) × 3 -4 µm. Culture characteristics -Colonies erumpent, spreading, with moderate aerial mycelium and smooth, lobate margin, reaching 50 mm diam after 2 wk at 25 °C. On MEA, PDA and OA surface and reverse buff. 1; Identi-ties = 508/536 (95 %), six gaps (1 %)). Closest hits using the LSU sequence are Phaeothecoidea melaleuca (strain CPC 17223, GenBank HQ599595. 1; Identities = 831/844 (98 %), four gaps (0 %)), Xenoconiothyrium catenata (strain CMW 22113, GenBank JN712570.
1; Identities = 833 /849 (98 %), two gaps Notes -Phaeosphaeriopsis was introduced by Câmara et al. (2003) to accommodate several paraphaeosphaeria-like taxa. Phaeosphaeriopsis sansevieriae formed only an asexual morph in culture. It is closely related to P. obtusispora (ascospores 17-22 × 5 -6 µm, on leaves Yucca gloriosa, Argentina; Câmara et al. 2003), but is phylogenetically distinct from that species. (0 %)) and Neophaeothecoidea proteae (strain CBS 114129, GenBank MH874518. 1; Identities = 852/869 (98 %), no gaps). Distant hits obtained using the rpb2 sequence had highest similarity to Zasmidium eucalyptorum (strain CBS 118500, GenBank MF951702. 1; Identities = 377/492 (77 %), eight gaps (1 %)), Zymoseptoria tritici (strain ST99CH_3D1, GenBank LT854276. 1; Identities = 349/455 (77 %), ten gaps (2 %)) and Constantinomyces oldenburgensis (strain T2.
4, GenBank LT976528. 1; Identities = 618/855 (72 %), 34 gaps (3 %)). Clos- est hits using the tub2 sequence had highest similarity to Lapi- domyces hispanicus (strain TRN500, GenBank KF546778. 1; Identities = 338 /407 (83 %), seven gaps (1 %)), Cladosporium sphaerospermum (strain UBOCC-A-101110, GenBank KJ596619. 1; Identities = 344/415 (83 %), 20 gaps (4 %)) and Cladosporium salinae (strain EXF-322, GenBank EF101403. 1; Identities = 396/496 (80 %), 32 gaps (6 %)). Colour illustrations. Sansevieria hyacinthoides. Left column: Phaeo- sphaeropsis sansevieriae. Conidiomata on OA; conidiogenous cells giving rise to conidia; conidia. Right column: Lasionectria sansevieriae. Conidio- Colour illustrations. Aloidendron dichotomum. Colony on SNA; conidio-genous cells giving rise to conidia; conidia.
Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: don. cowan@up. ac. za Gillian Maggs-Kölling, Gobabeb-Namib Research Institute, Walvis Bay, Namibia; e-mail: gillian@gobabeb. org Typus. Namibia, Gobabeb Namib Research Institute, Mirabib, on persis- tent inflorescence remains of Blepharis obmitrata (Acanthaceae), 19 Nov. 2019, P. W. Crous, HPC 3109 (holotype CBS H-24492, culture ex-type CPC 38944 = CBS 146980, ITS, LSU, actA, tef1 (first part) and tef1 (second part) sequences GenBank MZ064440. 1, MZ064497. 1, MZ078152. 1, MZ078228. 1 and MZ078253. 1, MycoBank MB 839527). Colour illustrations. Inflorescence remains of Blepharis obmitrata. Colony on SNA; hyphae giving rise to endoconidia.
On MEA and PDA surface smoke grey, reverse olivaceous grey; on OA surface olivaceous grey. Typus. Namibia, Gobabeb Namib Research Institute, Mirabib, on dung of Procavia capensis, 19 Nov. 2019, P. W. Crous, HPC 3110 (holotype CBS H-24493, culture ex-type CPC 38946 = CBS 146981, ITS, LSU, tef1 (first part) and tub2 sequences GenBank MZ064441. 1, MZ064498. 1, MZ078229. 1 and MZ078269. 1, MycoBank MB 839528).
1; Identities = 800/835 (96 %), five gaps (0 %)) and Muellerites juniperi (strain CBS 339. 73, GenBank MH877745. 1; Identities = 825/864 (95 %), six gaps (0 %)). Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P. O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p. crous@wi. knaw. nl & e. groenewald@wi. knaw. nl Angus J. Carnegie, Forest Health, NSW Department of Primary Industries, Level 30, 12 Darcy St, Parramatta NSW 2150, Australia; e-mail: angus. carnegie@dpi. nsw. gov. au Colour illustrations. Eucalypt forest. Colonies on SNA; hyphae with conidiogenous loci; conidia. Scale bars = 10 µm.
1; Identities = 278/293 (95 %), no gaps) and Paramyrothecium terrestris (strain CBS 564. 86, GenBank KU846420. 1; Identities = 277/294 (94 %), one gap (0 %)). Also see the phylogenetic tree provided with the supplementary material FP1194. Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P. O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p. crous@wi. knaw. nl & e. groenewald@wi. knaw. nl Neriman Yilmaz, Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: neriman. yilmazvisagie@fabi. up. ac. za Don Cowan, Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria,.
2015, J. Roux, HPC 3247 (holotype CBS H-24552, culture ex-type CPC 39351 = CBS 147084, ITS, LSU, rpb2 and tef1 (first part) sequences GenBank MZ064455. 1, MZ064512. 1, MZ078212. 1 and MZ078236. 1, MycoBank MB 839542). Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P. O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p. crous@wi. knaw. nl & e. groenewald@wi. knaw. nl Jolanda Roux, Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: jolanda. roux@gmail. com Colour illustrations. Forest at Gundari, Miombo. Conidiomata on OA; conidiogenous cells giving rise to conidia; conidia.
Laurus nobilis on the Isles of Scilly, and later on Arbutus andrachnoides and Gaultheria shallon in West Devon, UK. Lechat et al. (2010)linked this disease to a new species, Calonectria lauri (validated byLiu et al. 2020), which was reported from leaves of Ilex aquifolium in France and the Netherlands, and roots of Buxus sempervirens in Belgium. Calonectria lauri is sister to C. californiensis described in the present study. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence of CPC 29619 had highest similarity to Calonectria lauri (strain CMW 23682 (R), GenBank MT359736. 1; Identities = 534/534 (100 %), no gaps), Calonectria citri (strain CMW 23675 (R), GenBank MT359683. 1; Identities = 533/534 (99 %), no gaps) and Calonectria tunisiana (now synonym of Calonectria pseu- domexicana; strain CAL-EU2, GenBank MT345681.
1; Identities = 531/534 (99 %), no gaps). The ITS sequences of CPC 29619, 29620 and 29622 are identical (533/533 (100 %)). Closest hits using the LSU sequence are Calonectria lauri (strain CMW 23682 (R), GenBank MT359496. 1; Identities = 821/821 (100 %), no gaps), Calonectria penicilloides (strain CMW 23696 (R), GenBank MT359559. 1; Identities = 819/821 (99 %), no gaps) and Calonectria leucothoes (strain CMW 30977 (R), GenBank MT359498. 1; Identities = 819/821 (99 %), no gaps). The LSU sequences of CPC 29619, 29620, 29621 and 29622 are identical (781/781 (100 %)). Closest hits using the actA sequence of CPC 29619 had highest similarity to Calonectria lauri (strain CMW 23682 (R), GenBank MT335043. 1; Identities = 221/221 (100 %), no gaps), Calonectria citri (strain CMW 23675 (R), GenBank MT334992.
1; Identities = 213/222 (96 %), one gap (0 %)) and Calonectria leucothoes (strain CMW 30977 (R), GenBank MT335045. 1; Identities = 214/222 (96 %), one gap (0 %)). The actA sequences of CPC 29619, 29620, 29621 and 29622 are identical (559/559 (100 %)). Closest hits us- Typus. uSa, California, Sonoma County, Guerneville, on leaves of Umbel-lularia californica (Lauraceae), 5 May 2015, S. Rooney-Latham, CDFA267 ing the cmdA sequence of CPC 29619 are Calonectria lauri (strain CMW 23682 (R), GenBank MT335275. 1; Identities = (holotype CBS H-24751, culture ex-type CPC 29621 = CBS 147604, LSU, 654/662 (99 %), no gaps), Calonectria citri (strain CMW 23675 actA, cmdA, his3, tef1 (first part) and tub2 sequences GenBank MZ064514. 1, (R), GenBank MT335222. 1; Identities = 627/663 (95 %), two MZ078156. 1, MZ078169.
1, MZ078182. 1, MZ078238. 1 and MZ078279. 1, MycoBank MB 839545). gaps (0 %)) and Calonectria leucothoes (strain CMW 30977 (R), GenBank MT335277. 1; Identities = 618/662 (93 %), no Additional material examined. uSa, California, Sonoma County, Guerne-ville, on leaves of U. californica, 5 May 2015, S. Rooney-Latham, CPC 29619, ITS, LSU, actA, cmdA, his3, rpb2, tef1 (first part) and tub2 sequences Gen-Bank MZ064457. 1, MZ064515. 1, MZ078157. 1, MZ078170. 1, MZ078183. 1, MZ078213. 1, MZ078239. 1 and MZ078280. 1; ibid. , CPC 29620, ITS, LSU, actA, cmdA, his3, tef1 (first part) and tub2 sequences GenBank MZ064458. 1, gaps). The cmdA sequences of CPC 29619, 29620 and 29622 are identical (662/662 (100 %)). Closest hits using the his3 sequence of CPC 29619 had highest similarity to Calonectria lauri (strain CMW 23682 (R), GenBank MT335515.