Further studies are needed to provide a fuller understanding of how RP mRNA translation in STAR+METHODS Continued REAGENT or RESOURCE KEY RESOURCES TABLE Rnase T1 SOURCE Ambion IDENTIFIER Cat#EN0541 n-Octylglucoside Sigma-Aldrich Cat#10634425001 Critical Commercial Assays REAGENT or RESOURCE NeuroPORTER transfection reagent SOURCE Sigma-Aldrich IDENTIFIER Cat#NPT01 Antibodies Rneasy mini kit QIAGEN Cat#74104 Rabbit polyclonal anti-Rps4x SuperScript II First Strand Synthesis kit Proteintech Thermo Fisher Scientific Cat#14799-1-AP; RRID: AB_2238567 Cat#18080051 Rabbit polyclonal anti-Rpl17 Quantitect SYBR Green PCR kit Proteintech QIAGEN Cat#14121-1-AP; RRID: AB_2253985 Cat#204141 Rabbit polyconal anti-Rps14 KAPA HyperPrep kit Abcam Roche Cat#ab174661 Cat#KK8503 Mouse monoclonal anti-Rpl19 NextSeq 500/550 high output v2 kit (150 cycles) Abcam Illumina Cat#ab58328; RRID: AB_945305 Cat#FC-404-2002 Mouse monoclonal anti-Rps23 Duolink In Situ Detection Reagents Green Abcam Sigma-Aldrich Cat#ab57644; RRID: AB_945314 Cat#DUO92014 Rabbit polyclonal anti-Rps3a Duolink In Situ PLA probe Anti-Rabbit PLUS Proteintech Sigma-Aldrich Cat#14123-1-AP; RRID: AB_2253921 Cat#DUO92002 Mouse monoclonal anti-Neurofilament A Duolink In Situ PLA probe Anti-Mouse MINUS DSHB Sigma-Aldrich Cat#3A10; RRID: AB_531874 Cat#DUO92004 Rabbit polyclonal anti-Rpl39 Click-iT Cell Reaction Buffer Kit Abcam Thermo Fisher Scientific Cat#ab74740; RRID: AB_1524345) Cat#10269 Rabbit polyclonal anti-Rps12 Deposited Data Proteintech Cat#16490-1-AP; RRID: AB_2146233 Rabbit monoclonal anti-Gtpbp4 Proteomics data Abcam This study Cat#ab92342; RRID: AB_2049721 PRIDE: PXD011032 Mouse monoclonal anti-Npm1 Origene Cat#BM5524; RRID: AB_1008764 PRIDE: PXD015574 Rabbit polyclonal anti-Abce1 RNA-sequencing data Abcam This study Cat#ab32270; RRID: AB_722514 GSE135502 Rabbit polyclonal anti-b-actin Experimental Models: Organisms/Strains Abcam Cat#ab8227; RRID: AB_2305186 Rabbit polyclonal anti-b-catenin Xenopus laevis Sigma-Aldrich Nasco Cat#C2206; RRID: AB_476831 Cat#LM00715, Cat#LM00535 Mouse monoclonal anti-puromycin, clone 12D10 Oligonucleotides Sigma-Aldrich Cat#MABE343; RRID: AB_2566826 Mouse monoclonal anti-ribosomal RNA (Y10B) Morpholino: Control MO 5 0 -CCTCTTACCTCAGTTA Abcam Cat#ab171119 Rabbit monoclonal anti-biotin CAATTTATA-3 0 Cell Signaling Cat#5597; RRID: AB_10828011 Goat anti-rabbit Alexa Fluor 568 Abcam Cat#ab150117; RRID: AB_2688012 Goat anti-mouse Alexa Fluor 594 Abcam Cat#ab150080; RRID: AB_2650602 Mouse monoclonal anti-puromycin, clone 12D10, Sigma-Aldrich Cat#MABE343-AF647 Alexa Fluor 647 Conjugate Goat anti-mouse HRP Abcam Cat#ab6789; RRID: AB_955439 Goat anti-rabbit HRP Abcam Cat#ab97080; RRID: AB_10679808 Bacterial and Virus Strains BioBlue Chemically Competent Cells Bioline Cat#BIO-85037 Chemicals, Peptides, and Recombinant Proteins AHA (L-Azidohomoalanine) ThermoFisher Cat#C10102 Leibovitz's L-15 Medium ThermoFisher Cat#11415064 Leibovitz's L-15 medium -Lys -Arg GIBCO Life technologies N/A (customized) Stable isotope-coded amino acids Lys8 Silantes GmbH Cat#211603902 Stable isotope-coded amino acids Arg10 Silantes GmbH Cat#20160390 Antibiotic-Antimycotic (100X) Thermo Fisher Scientific Cat#15240062 Poly-L-lysine Sigma-Aldrich Cat#P1274 Laminin Sigma-Aldrich Cat#L2020 Puromycin Sigma-Aldrich Cat#P8833 Cycloheximide Sigma-Aldrich Cat#C4859 Anisomycin Sigma-Aldrich Cat#A9789 Cy5-UTP PerkinElmer Cat#NEL582001EA SUPERase In RNase Inhibitor Ambion Cat#AM2696 FluorSave Merck Millipore (Calbiochem) Cat#345789-20 Duolink In Situ Mounting Medium with DAPI Sigma-Aldrich Cat#DUO82040-5ML Recombinant mouse Netrin-1 R&D systems Cat#1109-N1 RNase A Ambion Cat#EN0531 (Continued on next page) e1 Cell Reports 29, 3605-3619.