dataesr/scientific-paragraphs-categorization huggingface dataset:
A. 2 -Environmental variables selected for Species Distribution Models (SDM) of Aves and Papilionidae species. Variables used for SDM are the variables with 835 collinearity removed (Pearson >0. 7). 836 Type of variables Variables Database Year Raw precision Source Variables used for SDM Climatic 19 bioclimatic variables Chelsa 1981-2010 1 km Karger et al. , 2021, 2017 Bio 2 (temp. range), Bio 5 (temp. warmest month), Bio 6 (min. temp. coldest month), Bio 9 (temp. driest quarter), Bio 13 (prec. wettest month), Bio 14 (prec. driest month), Bio 15(prec. seasonality) Elevation, mean in a buffer of fifty meters Elevation Biogeography Elevation Slope, mean in a buffer of 50 meters BD ALTI, IGN 2014 25 m IGN, 2022 Slope Topographic Position Index (TPI), mean in a buffer of 50 meters TPI Wetland Area of wetland potential: very high in a buffer of 50, 500, 2000 meters INRAE Wetland potential 2014 50 m Berthier et al.
1. A -Local scale (10km buffer zone). B. 1. C -France scale. France Taxa Statistic T1 GBIF T2 T3 T1 Vigie Nature T2 T3 All databases combined T1 T2 T3 nSp 14 2 11 14 2 11 Amphibia pSp<15obs pSp15. 50obs 7% 0% 0% 0% 0% 9% No program 0% 7% 0% 0% 0% 9% pSp. >50obs 93% 100% 91% 93% 100% 91% nSp 204 170 131 201 170 132 205 171 132 Aves pSp<15obs pSp15. 50obs 2% 3% 1% 1% 1% 1% 8% 11% 9% 9% 4% 7% 1% 1% 0% 1% 0% 0% pSp. >50obs 95% 98% 98% 81% 82% 89% 98% 99% 100% nSp 19 19 21 25 22 25 26 22 26 Chiroptera pSp<15obs pSp15. 50obs 90% 10% 89% 11% 90% 10% 0% 0% 0% 0% 0% 0% 0% 4% 0% 0% 4% 0% Taxa Amphibia Aves Chiroptera Flora Mammalia Odonata Statistc nSp Flora pSp<15obs pSp15. 50obs pSp. >50obs nSp Mammalia pSp<15obs pSp15. 50obs pSp. >50obs nSp Odonata pSp<15obs pSp15. 50obs pSp. >50obs nSp Orthoptera pSp<15obs pSp15.
50obs pSp. >50obs nSp Papilionidae pSp<15obs pSp15. 50obs pSp. >50obs pSp15. 50obs nSp Reptilia pSp<15obs pSp. >50obs GBIF nSp T1 T2 11 1 pSp<15obs pSp15. 50obs 82% 100% pSp. >50obs 18% 0% nSp 0% 0% 100 73 pSp<15obs pSp15. 50obs 87% 93% pSp. >50obs 10% 6% nSp 3% 1% 4 0 pSp<15obs pSp15. 50obs 100% -pSp. >50obs 0% -nSp 0% -786 476 pSp<15obs pSp15. 50obs 81% 85% pSp. >50obs 16% 14% nSp 3% 1% 10 1 pSp<15obs pSp15. 50obs 100% 100% pSp. >50obs 0% 0% nSp 0% 0% 26% 0% 38 5 pSp<15obs pSp15. 50obs 74% 100% pSp. >50obs 0% 1573 T3 11 25% 18% 100% 57% 0% 23 0% 77 13% 17% 95% 70% 4% 57 1% 5 2% 10% 100% 88% 0% 95 0% 639 22% 28% 81% 50% 17% 147 3% 14 4% 8% 100% 88% 0% 21 0% 0% 23 0% 14% 100% 86% 0% 1261 T1 13 20% 18% 23% 62% 15% 6 62% 197 16% 17% 37% 67% 23% 7 40% 21 0% 14% 10% 86% 38% 25 52% 1703 12% 20% 52% 68% 30% 18 18% 23 0% 0% 78% 100% 17% 4 4% 37% 57 0% 0% 28% 100% 0% SINP 995 T2 2 17% 16% 50% 67% 50% 28 0% 94 18% 28% 96% 54% 3% 30 1% 6 0% 0% 83% 100% 0% 34 17% 1335 6% 21% 48% 73% 25% 52 27% 7 0% 0% 86% 100% 14% 7 0% 0% 1 0% 0% 100% 100% 100% 1336 T3 0 68% 19% -13% --82 No program 100% Local 1105 T1 61% 23% 16% 114 85% 56% 15% 32% 57 7 0% 12% 18 25 11% 0% 26% 0% 50% 12% 63% 100% 44% 16% 25 19 6% 72% 843 286 4% 0% 0% 0% 74% 100% 100% Vigie Nature 96% 848 1844 T2 No program 53% 19% 27% 21% 20% 60% 23 155 9% 13% 50% 78% 17% 30 57 33% 22 0% 0% 13% 3% 41% 87% 97% 36% 19 98 23% 177 0% 15% 0% 25% 100% 96% 100% 100% 60% 14% 0% 0% 149 18 52 154 12% 0% 0% 30 No program 13% 5% 0% 1% 18% 6% 0% 7% 60% 69% 89% 100% 92% 20% 22 20% --0% 0 0 1 4% No program 5% --100% 91% 100% 1460 T3 14% 17% 69% 7 101 14% 29% 54% 57% 20% 7 27% 24 0% 0% 21% 100% 46% 25 33% 136 0% 4% 100% 96% 0% 18 0% 0% 0% 100% 4 0% 24 0% 0% 100% 100% 96% 1093 All databases combined T1 T2 T3 14 2 16% 16% 11 29% 50% 68% 100% 7% 50% 30 0% 64% 0% 0% 205 171 23% 17% 132 37% 47% 60% 59% 22% 22% 30 13% 41% 31% 28% 26 22 0% 0% 26 12% 41% 100% 27% 19% 36% 34 34% 69% 23% 39% 1844 1460 0% 9% 1093 52% 50% 91% 72% 28% 24% 52 17% 20% 25% 11% 23 7 0% 0% 30 74% 86% 100% 57% 22% 14% 7 20% 4% 0% 23% 30% 0% 3% 57 7 0% 0% 30 28% 100% 97% 100% pSp.
>50obs 0% 0% 0% 35% 0% - - 0% 0% 42% 0% 0% nSp 53 3 24 91 8 0 25 19 19 98 25 34 Orthoptera pSp<15obs pSp15. 50obs 92% 8% 100% 0% 100% 0% 71% 23% 100% 0% -- 24% 24% 58% 32% 16% 52% 56% 18% 68% 24% 53% 29% pSp. >50obs 0% 0% 0% 6% 0% - 52% 10% 32% 26% 8% 18% nSp 115 15 33 154 11 0 0 0 50 154 18 52 Papilionidae pSp<15obs pSp15. 50obs 60% 30% 100% 0% 94% 6% 21% 19% 100% 0% -- -- -- 88 12 21% 18% 100% 0% 73% 27% pSp. >50obs 10% 0% 0% 60% 0% - - - 0 60% 0% 0% nSp 18 2 7 21 4 0 22 4 7 Reptilia pSp<15obs pSp15. 50obs 67% 28% 100% 0% 100% 0% 24% 33% 100% 0% -- No program 27% 32% 75% 25% 100% 0% pSp. >50obs 6% 0% 0% 43% 0% - 41% 0% 0% B. 1. A -Regional scale. Regional Taxa Statistic T1 GBIF T2 T3 T1 Vigie Nature T2 T3 All databases combined T1 T2 T3 nSp 13 2 11 14 2 11 Amphibia pSp<15obs pSp15. 50obs 8% 23% 0% 50% 73% 18% No program 7% 14% 0% 50% 73% 18% pSp.
Patient and microbiological characteristics Demographic data, predisposing conditions, concomitant infections, initial symptoms, intra-hospital complications, use for corticosteroid and cerebral imaging were prospectively collected in an electronic CRF. Immunosuppressed patients with congenital or acquired immunodeficiency, asplenic patients, patients under immunosuppressive drugs and patients with HIV infection were considered immunocompromised. Data including CSF culture, blood culture, and pathogen identification were collected. Microbiological analyzes were carried out locally in hospital microbiology laboratories and bacterial strains were also sent to the national reference center for each corresponding microorganism.
Multivariate analysis was performed by a logistic regression model. Variables which degree of bivariate significance was below 0. 20 were initially introduced into the model. The final model retained only variables which significance level was less than or equal to 0. 05. We assessed the linearity of the association between continuous variables and outcome with the Lemeshow goodness of fit and by visual inspection. In order to better analyze the role of corticosteroid initiation, a sensitivity analysis was performed excluding patients with symptomatic CVC already present at hospital admission or occurring during the 48 first hours. Statistical analyzes were performed using the STATA Version 13 software and variables with a p statistic less than 0. 05 were considered statistically significant.
Second, the radiological data did not make possible the distinction between venous and arterial infarctions because of the non-systematically contrast-injected nature of the images. Third, there was no independent reading of brain imaging by a neuro-radiologist. Fourth, the size of the different subgroups (definite symptomatic/definite asymptomatic/ probable) was too small to allow stratified multivariate analyses. Finally, we did not have data on cardiovascular risk factors and the precise date of onset of cerebrovascular complications, which that were collected only by period (before 48 h, after 48 h). In conclusion, we found that CVC in adult patients with community-acquired bacterial meningitis were frequent. Advanced age, alteration of consciousness on admission and seizures during hospitalization were independent factors associated with the occurrence of CVC whether dexamethasone use was not.
Preparatory phase The surgical preparation, anesthesia, data monitoring, and recording procedures used in the studies performed during the inclusion period have been previously described. 19, 20  Under aseptic surgical conditions, we initially sedated the pigs with intramuscular ketamine (10 mL of 100 mg/mL) followed by inhaled isoflurane (0. 8-1. 2%). Pigs were intubated with a size 7. 0 endotracheal tube then ventilated with room air, using a ventilator (Narkomed, Telford, Pennsylvania) with a tidal volume of 10 ml/kg and a respiratory rate adjusted to continually maintain a PaCO2 of 40 mmHg and PaO2 around 80 mmHg (blood oxygen saturation >95%). Central aortic blood pressure was recorded continuously with a catheter (Mikro-Tip Transducer, Millar Instruments, Houston, Texas) placed in the descending thoracic aorta.
A second Millar catheter was inserted in the right atrium via the right internal jugular vein to measure right atrial (RA) pressure. Animals received up to 1000 ml of normal saline solution after surgical preparation in order to maintain a mean right atrial pressure between 3-5 mmHg. Animals received an intravenous heparin bolus (100 units/kg). Hemodynamic data and telemetry were continuously monitored and recorded (BIOPAC MP 150, BIOPAC Systems, Inc. , CA, USA). Coronary perfusion pressure (CPP) was calculated as the difference between aortic and right atrial diastolic pressures during the decompression phase of CPR. End-tidal carbon dioxide, tidal volume, and blood oxygen saturation were continuously measured (COSMO Plus, Novametrix Medical Systems, Wallingford, Connecticut). ROSC was defined using the Utstein guidelines for uniform reporting in animal research as maintenance of systolic pressure of 60 mm Hg for 10 consecutive minutes.
is recommended for recording. This information will help research scientists to verify the sampling procedure and data interpretation. The citizen science approach can undoubtedly provide valuable insights into TBD ecology and epidemiology. This study utilised a national citizen science-based volunteer network to investigate the B. burgdorferi s. l. and B. miyamotoi prevalence in tick pools across Great Britain. We describe a project here that combines citizen science with PCR methods for detecting bacteria in ticks. Citizen scientists sent ticks they found from various geographic locations using our tick kit. Ticks from the same location, collected on the same date and from the same origin were pooled together. The tick pools were analysed using our newly developed Bmer-qPCR to detect B. miyamotoi and our previously established Ter-qPCR for B.
Construction of the positive control plasmid DNA A plasmid carrying the phage terL gene fragment from B. miyamotoi (named as Bmer-plasmid) was constructed by the The Bmer-qPCR Detailed information regarding the primers and probe used in this study can be found in the approved patent (Ref. P184103. EP. 01/T. ) (Shan et al. , 2018). Briefly, a pair of PCR primers and a probe (the Bmer-qPCR) were designed using the PrimerQuest ® Tool to amplify a 120 bp region of the Borrelia miyamotoi FR64b terL gene (GenBank accession CP004220. 1), The primers were F terBm :AGCCTACCTAGATCCTGCTTAT and R terBm :GGGTCACTT GCTGGTAGTTT. The probe was P terBm :AGTGCACTTTGTGTGCT TGAAATGGT. The fluorogenic probe was labelled with 6-carboxyfluorescein (FAM) fluorescent reporter dye at the 5′-end, an internal ZEN™ Quencher and an Iowa Black Fluorescent Quencher (IBFQ) to the 3′ (5'FAM/ZEN/3'IBFQ).
PCR setup The Bmer-qPCR and Ter-qPCR were conducted according to our established protocol  (Shan et al. , 2021). Briefly, each primer and probe at a final concentration of 0. 5 and 0. 25 μM, respectively, were added in a 20 μl final reaction volume containing 10 μl 2X PrimeTime Master Mix, with 4 μl template DNA, and nuclease-free water. The qPCR result was analysed using FAST7500 software v2. 3. For a qPCR result to be valid, the non-template control (NTC) and the extraction control should be negative, whilst positive controls of 100 copies of Bmer-plasmid and Ter-plasmid  (Shan et al. , 2021) , respectively should produce typical qPCR amplification curve with Cq values below 30. When analysing tick pools, positive qPCR results were defined as those with typical PCR curves and Cq ≤ 38. For samples with typical qPCR curves but displaying Cq between 38 and 40, PCR products were gel purified and sequenced (Source Bioscience).
The terL gene within Borrelia miyamotoi species The terL gene (CP004220. 1:11820-13172) was discovered from B. miyamotoi FR64b. Blastn search confirmed that its homologues (with E value of 0, 100% Query Cover and > 90% Percent Identity) were widespread within B. miyamotoi strains, such as American B. miyamotoi strain LB2001, CT13-2396, European B. miyamotoi strain NL-1R-1 and Russian B. miyamotoi strain Izh-5 (CP024217. 1:5875-7227) and B. miyamotoi strain Yekat-18 (CP037516. 1:247-1598). The terL gene encodes for the terminase large subunit, which is an essential component of the phage packaging system. Phages are viruses that infect bacteria, and they use the host bacterium's machinery to replicate and package their own genetic material. The terminase is responsible for recognising and cutting the viral DNA into small segments, which are then packaged into new phage particles  (Sun et al.
Probit analysis via the SPSS package with 95% probability was used to estimate the LOD and was found to be 10 copies per PCR  (Pavšič et al. , 2016). This means that the assay can detect as low as 10 copies of B. miyamotoi DNA per PCR reaction with a 95% confidence interval. Overall, the validation results of the Bmer-qPCR assay for the detection of B. miyamotoi DNA suggest that the Bmer-qPCR assay is a reliable and sensitive tool for the detection of B. miyamotoi DNA.
The campaign for a citizen scientist-based approach launched in April 2016. Since July 2016, we started receiving requests for our tick kits. We sent out 235 tick kits by post. Each kit has space for 1-10 tick collection tubes depending on the request. The data presented in this study represents tick collection from March to August 2017 (the tick season). As of 30 August 2017, 211 tick kits had been returned. Amongst these, 153 tick kits from 66 geographical locations (Supplementary Table  1 ) recorded accurate locations with 838 correctly identified adult ticks (multiple tick kits from the same location in different date and/or tick origins were received). Ticks from the same tick kit (representing ticks collected from the same location, on the same date and from the same origin as illustrated in Supplementary Table  1 ) were pooled together to form 153 tick pools, representing 66 geographical locations (6 regions in England and 5 regions in Scotland).
S3 ). Therefore, conversely to the Denisovan individual from Cobra Cave, the TPL H. sapiens consumed food from a more forested area. Despite the scarcity of archaeological material and poor organic matter preservation in tropical latitudes, we now have evidence of rainforests occupation by H. sapiens in Asia by ~ 70 ka  45, 50, 58, 59  and increasing evidence underlying the reliance on diverse settings ~ 45 ka  46, [60] [61] [62]. δ 13 C values of H. sapiens from all these sites highlight the capacity of our species to adopt various behaviours in similar environments where both C 3 forest and C 4 open biomes are present in the vicinity. The data suggest specialization, such as hunting of arboreal species  63  , use of coastal resources  64  , opportunistic use of resources from mosaic and/or open forest edge environments  65.
The shift in vegetation cover documented by the expansion of the canopy forest biome at Coc Muoi (148-117 ka), seems to coincide with the weaker monsoon interval 135. 5-129 ka (MIS 6. 2 in the Hulu/Sanbao records  75  ). This shift can be associated with the abrupt reappearance of forest montane elements in lowland zones with cool and relatively wet conditions  53. Considering the subsistence strategy of the Denisovan individual from Cobra Cave, could this cold event have been a driving factor in archaic hominin evolution? If data from a single individual cannot fully reflect the diversity of the subsistence of the entire group locally, the fact that it relied on mixed to open landscapes for food resources supports the idea that these biomes might have played a significant role in the mobility and settlement of this archaic hominin in a tropical ecosystem  77.
Statistical analyses Kruskal-Wallis one-way analysis of variance and post-hoc Games-Howell pairwise comparisons (Supplementary Tables S6, S7) were performed across the dataset to identify statistical differences in δ  13  C carbon source and δ  18  O apatite values between sites (and thus also periods). Preliminary tests and visual inspection were carried out to check for normally distributed data and equal variance, which revealed that non-parametric testing (i. e. , Kruskal-Wallis) was preferred over parametric methods (i. e. , ANOVA). A total of 6 different sites and 390 samples were used for the analyses: Cobra Cave (n = 55), Coc Muoi (n = 84)  29  , Tam Hang South (n = 62)  29  , Duoi U'Oi (n = 60)  29  , Nam Lot (n = 57)  49  and Tam Hay Marklot (n = 72)  48. All statistical analyses were conducted using the free program R software (version 4.
Acknowledgements We thank the  Ministry of Information, Culture and Tourism of Laos PDR  for encouraging and supporting our work. We thank also the authorities of  Xon district, Huà Pan Province , and the villagers of  Long Gua Pa village  for their continuous support of our fieldworks since 2003. Funding for isotopic analyses of the Cobra Cave specimens came from the research laboratory  BABEL UMR 8045 CNRS/Université Paris Cité, France , to A. M. B. and the  Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany , to N. B.
Methods This retrospective study was approved by the Ethics Committee of Angers University Hospital (2022/174) and was conducted in compliance with the Helsinki Agreement. Data collection was approved by the French Data Protection Authority (CNIL). We retrospectively reviewed all medical records of patients diagnosed with PCS who attended the outpatient clinic of the Internal Medicine Department of Angers University Hospital, France between June 2020 and June 2022, and were followed up until December 2022. We included all patients who fulfilled the WHO definition of PCS  [1]. We excluded patients who had medical records with missing or incomplete data, especially about pacing adherence and patients who were lost to follow-up. The initial and different follow-up assessments of all patients were standardized and conducted by the same physician.
Results Among the 106 patients who fulfilled the inclusion criteria, 20 patients were excluded (12 with missing medical record data and 8 lost to follow-up). In total, 86 patients were included with a male to female ratio of 1:4. 4. The median age at disease onset was 41  [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] [43] [44] [45] [46] [47] [48]  years, and the median time of follow-up was 10 [6-13] months. Fatigue was the most frequent symptom, reported by 84/86 (97. 7%) patients, followed by myalgia (53/86, 61. 6%) and cognitive impairment (50/86, 58. 1%). PEM was found in 32/86 (37. 2%) patients (Additional file 3). The followup of patients showed that 29/86 (33. 7%) experienced recovery and returned to work (R group), 20/86 (23. 3%) showed improvement in their health status (I group), and 37/86 (43%) did not recover or improved (no-R/I group) (Fig.
Atmospheric forcing data The FSM2 was forced with the SAFRAN meteorological system reanalysis dataset for flat slopes  (Vernay et al. , 2022). The SAFRAN meteorological system integrates meteorological simulations, remote-sensing cloud cover data, and instrumental records through data-assimilation. SAFRAN is forced with a combination of homogenized  ERA-40 reanalysis (1958 ERA-40 reanalysis ( to 2002) )  and the numerical weather prediction model ARPEGE (  2002 to 2020). SAFRAN system was firstly designed for avalanche monitoring  (Durand et al. , 1999 (Durand et al. , , 2009)) , but the accurate results obtained enhanced the diffusion of the meteorological system and its integration in the French hydrometeorological modelling system by the local weather service, Metéo-France  (Habets et al.
Spatial areas SAFRAN system provides data at hourly resolution from 0 to 3600 m, by steps of 300 m, grouped by massifs. The SAFRAN massifs (polygons of Figure  1 ) were chosen for their relative topographical and climatological similarities  (Durand et al. , 1999). We selected the 1500 m (low), 1800 m (mid), and 2400 m (high) specific elevation bands of the Pyrenees. In order to retain the main spatial differences across the mountain range, reduce data dimensionality and include the maximum variance, massifs with similar interannual snow characteristics were grouped into sectors by performing a Principal Component Analysis (PCA). PCA is an extensively applied statistical method for climatological and snow spatial regionalization (i. et al. , 2022a). A PCA was applied over HS data for all months and years of the baseline climate.
Acknowledgements This work frames within the research topics examined by the research group " Antarctic, Artic, Alpine Environments-ANTALP " ( 2017-SGR-1102 ) funded by the  Government of Catalonia, HIDROIBERNIEVE  ( CGL2017-82216-R ) and  MARGISNOW  ( PID2021-124220OB-100 ), from the  Spanish Ministry of Science, Innovation and Universities. JB is supported by a  pre-doctoral University Professor FPI grant  ( PRE2021-097046 ) funded by the  Spanish Ministry of Science, Innovation and Universities. Verfaillie,  D. ,  Lafaysse ,  M. ,  Déqué ,  M. ,  Eckert , N. , Lejeune, Y. , and  Morin , S. : Multi-component ensembles of future meteorological and natural snow conditions for 1500 m altitude in the Chartreuse mountain range, Northern French Alps, Cryosphere, 12, 1249-1271, https://doi. org/10.
Data availability FSM2 is an open access snow model (Essery, 2015)  provided at https://github. com/RichardEssery/FSM2 (last access 15 January 2023). SAFRAN climate dataset (Vernay et al. , 2022)  is available by AERIS at https://www. aeris-data. fr/landing-page/?uuid=865730e8-edeb-4c6b-ae58-80f95166509b#v2020. 2 (last access 16 December 2022). Data of this work is available upon request by the first author (josepbonsoms5@ub. edu).
Ethics Consecutive control plasma apheresis (FFP) was provided by non-convalescent donors eligible for plasma donation and who similarly underwent pathogen reduction. All CCP and control plasma donors provided written informed consent, and enrolled in a multiple site trial with similar preparation and collection procedures between May and November 2020. The COVID-19 CP donor collection was approved by the French National Ethics Committee. Written informed consent was obtained from all the patients or their trusted persons. Data collection from the PLASMACOV cohort was approved by the French National Ethics Committee (2020-A00728-31). 15, 16 No study protocol is available.
Functional evaluation of anti-cytokine autoantibodies Luciferase reporter assays: The blocking activity of anti-IFN-α2 and anti-IFN-ω auto-Abs was determined with a reporter luciferase activity. 12 Briefly, HEK293T cells (RRID:CVCL_0063) were transfected with a plasmid containing the firefly luciferase gene under the control of the human ISRE promoter in the pGL4. 45 backbone, and a plasmid constitutively expressing Renilla luciferase for normalisation (pRL-SV40P -RRID:Addgene_27163). Cells were transfected in the presence of the X-tremeGene 9 transfection reagent (Sigma Aldrich, St. Quentin Fallavier, France) for 36 h. Cells in Dulbecco's modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% foetal calf serum (FCS) and 10% healthy control or patient serum/plasma were stimulated with IFN-α2 (Milteny Biotec, Paris, France), IFN-ω (Merck, St.
Endothelial cell culture EA. hy926 (RRID:CVCL_3901) is a permanent cell line derived by fusing human umbilical vein endothelial cells (HUVEC) with cell line A549. 17 EA. hy926 was cultured as described. 18 Cells were cultured in 6-well and 96-well plates in order to obtain approximately 10 6 cells/ mL. The cell count was quantified with a TC10 Automated Cell Counter (Bio-Rad, Marnes-la-Coquette, France). Cells were grown until confluent then passaged with 0. 25% trypsin (Sigma-Aldrich, Saint-Quentin-Fallavier, France). Using cultured human pulmonary microvascular endothelial cells (HPMVEC), we assessed whether CCP and FFP could trigger endothelial damage in vitro as described previously. 19.
Cells were centrifuged at 300 g for 5 min at 22 • C and then washed in PBS. Data acquisition was performed using a Guava easyCyte HT Flow Cytometer (Merck Millipore, Molsheim, France) and the data were analysed using the Incyte programme. At least 10,000 events were collected for each sample. Mycoplasma testing was performed to confirm the absence of contamination according to the recommended protocol (MycoStrip™-Mycoplasma Detection Kit # rep-mys-50 InvivoGen).