dataesr/scientific-paragraphs-categorization huggingface dataset:
Culture characteristics -Colonies erumpent, spreading, with moderate aerial mycelium and smooth, lobate margin, reaching 7 mm diam after 2 wk at 25 °C. On MEA, PDA and OA surface olivaceous grey, reverse iron-grey. Notes -Paramycosphaerella syzygii forms a passalora-like asexual morph in culture. On the host tissue, however, it formed synnemata, which were absent in culture. Videira et al. (2017)  commented that Paramycosphaerella was heterogeneous, and although most species were known by their sexual morphs, some had zasmidium-or pseudocercospora-like asexual morphs. Phylogenetically and morphologically, P. syzygii is distinct from other species described in the genus. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Paramycosphaerella dicranopteridis (strain TNM 3953, GenBank NR_155639.
1; Identities = 478/507 (94 %), five gaps (0 %)), Paramycosphaerella blechni (strain CPC 24698, GenBank NR_155663. 1; Identities = 478/513 (93 %), ten gaps (1 %)) and Mycosphaerella rosigena (strain CBS 330. 51, GenBank GU214632. 1; Identities = 476/511 (93 %), nine gaps (1 %)). Closest hits using the LSU sequence are Paramycosphaerella brachystegiae (strain CBS 136436, Gen-Bank NG_058048. 1; Identities = 859/866 (99 %), no gaps), Hyalozasmidium aerohyalinosporum (strain CPC 14636, Gen-Bank NG_059440. 1; Identities = 838/846 (99 %), no gaps), Virosphaerella irregularis (strain CBS 123242, GenBank MH874810. 1; Identities = 843/853 (99 %), no gaps) and Piricauda paraguayensis (strain VIC 31785. 52, GenBank KJ459712. 1; Identities = 790/795 (99 %), no gaps). Closest hits using the actA sequence had highest similarity to Mycosphaerella musae (strain CIRAD-AUS, GenBank MW070780.
1; Identities = 536/601 (89 %), 15 gaps (2 %)), Paramycosphaerella watsoniae (strain CBS 146064, GenBank MN556790. 1; Identities = 544/614 (89 %), 23 gaps (3 %)) and Paramycosphaerella marksii (strain CBS 110750, GenBank KF903404. 1; Identities = 486/544 (89 %), seven gaps (1 %)). Closest hits using the cmdA sequence had highest similarity to Hyalozasmidium aerohyalinosporum (strain CBS 125011, GenBank KF902788. 1; Identities = 328/367 (89 %), ten gaps (2 %)), Septoria steviae (strain NCSep12, GenBank MH532378. 1; Identities = 278/304 (91 %), no gaps) and Nothopassalora personata (strain IRAN 3479C, GenBank MN422408. 1; Identities = 283/312 (91 %), no gaps). No significant hits were obtained when the tef1 and tub2 sequences were used in blastn and megablast searches.
On MEA surface and reverse greenish olivaceous with patches of pale mouse grey; on PDA surface and reverse luteous with patches of greenish olivaceous; on OA surface pale mouse grey. Notes -Paramycosphaerella pterocarpi is related to Paramycosphaerella watsoniae (conidia aseptate, fusoid-ellipsoid, (3. 5 -)4 -5(-6) × 2 μm; Crous et al. 2019d), but can be distinguished based on its morphology, and phylogeny. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Paramycosphaerella watsoniae (strain CPC 37392, GenBank NR_166341. 1; Identities = 520/528 (98 %), one gap (0 %)), Paramycosphaerella sticheri (strain CPC 24720, GenBank NR_155660. 1; Identities = 485/511 (95 %), two gaps (0 %)) and Paramycosphaerella wachendorfiae (strain CPC 18338, GenBank NR_156547.
1; Identities = 481/516 (93 %), eight gaps (1 %)). Closest hits using the LSU sequence are Paramycosphaerella brachystegiae (strain CBS 136436, Gen-Bank NG_058048. 1; Identities = 864/866 (99 %), no gaps), Paramycosphaerella watsoniae (strain CPC 37392, GenBank NG_068339. 1; Identities = 846/848 (99 %), no gaps) and Paramycosphaerella dicranopteridis-flexuosae (strain CPC 24743, GenBank NG_059577. 1; Identities = 805/808 (99 %), one gap (0 %)). Closest hits using the actA sequence had highest similarity to Paramycosphaerella watsoniae (strain CBS 146064, GenBank MN556790. 1; Identities = 552/584 (95 %), five gaps (0 %)), Mycosphaerella musae (strain CIRADCOK 003, GenBank MW070786. 1; Identities = 524/580 (90 %), five gaps (0 %)) and Paramycosphaerella intermedia (strain CBS 114356, GenBank KF903466.
1; Identities = 491/538 (91 %), four gaps (0 %)). Closest hits using the cmdA sequence had highest similarity to Paramycosphaerella watsoniae (strain CBS 146064, GenBank MN556795. 1; Identities = 405/428 (95 %), no gaps), Hyalozasmidium aerohyalinosporum (strain CBS 125011, GenBank KF902788. 1; Identities = 269/294 (91 %), no gaps) and Nothopassalora personata (strain IRAN 3479C, GenBank MN422408. 1; Identities = 278/309 (90 %), no gaps). Closest hits using the rpb2 sequence had highest similarity to Paramycosphaerella watsoniae (strain CBS 146064, GenBank MN556814. 1; Identities = 733/793 (92 %), no gaps), Virosphaerella pseudomarksii (strain CBS 123241, GenBank MF951686. 1; Identities = 679/864 (79 %), 26 gaps (3 %)) and Mycosphaerelloides madeirae (strain CBS 115936, GenBank KX288443. 1; Identities = 635/824 (77 %), ten gaps (1 %)).
Distant hits obtained using the tef1 sequence had highest similarity to Mycosphaerella musae (strain CIRAD-MTQ 1241, GenBank MW071103. 1; Identities = 315/381 (83 %), 24 gaps (6 %)), Brunneosphaerella nitidae (strain CPC 15231, GenBank JN712581. 1; Identities = 313/380 (82 %), 30 gaps (7 %)) and Brunneosphaerella protearum (strain CPC 18328, GenBank JN712585. 1; Identities = 307/378 (81 %), 27 gaps (7 %)). Closest hits using the tub2 sequence had highest similarity to Paramycosphaerella intermedia (strain CBS 114356, GenBank KF902845. 1; Identities = 275/329 (84 %), 12 gaps (3 %)), Paramycosphaerella marksii (strain CBS 110920, GenBank KF902848. 1; Identities = 270/328 (82 %), ten gaps (3 %)) and Zasmidium podocarpi (strain CBS 142529, GenBank KY979930. 1; Identities = 307/383 (80 %), 21 gaps (5 %)).
eucalypti (on Eucalyptus cyanophylla, Mildura, Mungo National Park, primary conidia 5 -12 × 3 -5 μm, becoming brown, thick-walled and swollen with age; Crous et al. 2018a), but is morphologically and phylogenetically distinct from the latter species. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Neocelosporium eucalypti (strain CBS 145086, GenBank NR_164293. 1; Identities = 559/578 (97 %), two gaps (0 %)), Celosporium larixicola (strain L3-1, GenBank FJ997287. 1; Identities = 495/544 (91 %), 11 gaps (2 %)) and Perusta inaequalis (strain CBS 118271, GenBank NR_144958. 1; Identities = 467/514 (91 %), 12 gaps (2 %)). Closest hits using the LSU sequence are Neocelosporium eucalypti (strain CBS 145086, GenBank NG_066297.
5 µm, smooth, straight to curved with conspicuous collarettes and periclinal thickening. Conidia aseptate, hyaline to olivaceous, subcylindrical, (8 -)10 -12(-13) × 2 -2. 5 µm, with obtuse ends. Culture characteristics -Colonies flat, spreading, surface with concentric zone lines, with moderate aerial mycelium and smooth, even margin, reaching 38 mm diam after 2 wk at 25 °C. On MEA, PDA and OA surface dirty white, reverse buff. Notes -Paramyrothecium salvadorae is related to P. roridum (conidia subcylindrical to ellipsoidal, (5 -)6. 5 -7. 5(-8) × 2 µm; Lombard et al. 2016), from which it is morphologically and phylogenetically distinct. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Paramyrothecium roridum (as Myrothecium roridum; strain MA-20, GenBank JF724152.
1; Identities = 579/ 584 (99 %), three gaps (0 %)), Paramyrothecium terrestris (strain CBS 564. 86, GenBank NR_145078. 1; Identities = 574/ 581 (99 %), one gap (0 %)) and Myrothecium lachastrae (strain IMI 273160, GenBank AY254159. 1; Identities = 570/578 (99 %), two gaps (0 %)). Closest hits using the LSU sequence are Paramyrothecium nigrum (strain CBS 116537, GenBank NG_069341. 1; Identities = 811/812 (99 %), no gaps), Paramyrothecium foliicola (strain CBS 419. 93, GenBank KU846323. 1; Identities = 811/812 (99 %), no gaps) and Paramyrothecium viridisporum (strain CBS 873. 85, GenBank NG_069344. 1; Identities = 810/812 (99 %), no gaps). Closest hits using the rpb2 sequence had highest similarity to Paramyrothecium sp. (strain MU6, GenBank MN397961. Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: don.
Supplementary material FP1221 Consensus phylogram (50 % majority rule) obtained from the maximum likelihood analysis with IQ-TREE v. 1. 6. 12 (Nguyen et al. 2015,  Kalyaanamoorthy et al. 2017, Hoang et al. 2018) of the Paramyrothecium and related genera multigene (ITS / tub2 / cmdA / rpb2) nucleotide alignment derived from the datasets of Lombard et al. (2016). GenBank accession numbers for the sequences used can also be obtained from Lombard et al. (2016). Bootstrap support values (> 69 % shown; only values > 94 % are significant) from 5 000 ultrafast bootstrap replicates are shown at the nodes. Culture collection numbers are indicated for all species and numbers in bold represent those cultures with a type status. The tree was rooted to Fusarium sambucinum (culture CBS 146. 95) and the species described here is highlighted with a coloured block and bold face.
5-8. 5(-10) × (5-)5. 5-6. 5(-7) μm; Lombard et al. 2016), from which it is distinct in having larger conidia. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Memnoniella oenanthes (strain IBT 9473, GenBank KX690115. 1; Identities = 489/498 (98 %), no gaps), Cymostachys coffeicola (strain CPC 25009, GenBank KU846053. 1; Identities = 503/513 (98 %), three gaps (0 %)) and Stachybotrys nephrospora (strain Stanep1907, GenBank KF626486. 1; Identities = 478/488 (98 %), one gap (0 %)). Closest hits using the LSU sequence are Cymostachys coffeicola (strain CBS 252. 76, GenBank MH872746. 1; Identities = 871/876 (99 %), one gap (0 %)), Stachybotrys nephrospora (strain LAMIC0040/07, GenBank KP893312. 1; Identities = 871/876 (99 %), two gaps (0 %)) and Cymostachys thailandica (strain CBS 145079, GenBank NG_066294.
Parateichospora phoenicicola Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Teichospora quercus (strain CBS 143396, GenBank NR_159069. 1; Identities = 432/490 (88 %), ten gaps (2 %)), Teichospora mariae (strain C136, GenBank KU601581. 1; Identities = 487/571 (85 %), 26 gaps (4 %)) and Teichospora kingiae (strain CPC 29104, GenBank NR_154656. 1; Identities = 485 / 569 (85 %, 24 gaps (4 %)). Closest hits using the LSU sequence are Misturatosphaeria minima (strain ANM 933, Gen-Bank GU385195. 1; Identities = 855/879 (97 %), two gaps (0 %)), Teichospora quercus (strain CBS 143396, Gen-Bank NG_067335. 1; Identities = 831/856 (97 %), two gaps (0 %)) and Teichospora proteae (strain CBS 122675, Gen-Bank EU552117. 1; Identities = 837/863 (97 %), three gaps (0 %)).
Closest hits using the rpb2 sequence had highest similarity to Teichospora rubriostiolata (strain C158x, Gen-Bank KU601597. 1; Identities = 628 /743 (85 %), two gaps (0 %)), Flabellascoma fusiforme (strain MFLUCC 18-1019, GenBank MT878452. 1; Identities = 496 /647 (77 %), 22 gaps (3 %)) and Flabellascoma minimum (strain KT 2040, GenBank LC312591. 1; Identities = 499/657 (76 %), 22 gaps (3 %)). Closest hits using the tef1 sequence had highest similarity to Teichospora melanommoides (strain MP5, GenBank KU601610. 1; Identities = 361/440 (82 %), 22 gaps (5 %)), Teichospora rubriostiolata (strain C158x, GenBank KU601608. 1; Identities = 363/443 (82 %), 23 gaps (5 %)) and Teichospora pusilla (strain C140, GenBank KU601605. 1; Identities = 360 /444 (81 %), 26 gaps (5 %)). Conidiomata pycnidial, black, globose, 80 -150 µm diam, with central ostiole; wall of 6 -8 layers of brown textura angularis; occurring as separate conidiomata on SNA and OA, but aggregated, immersed in a continuous black stroma on MEA.
Parateichospora phoenicicola Culture characteristics -Colonies flat, spreading, surface folded, with sparse aerial mycelium, and even, lobate margins, reaching 10 mm diam. On MEA surface and reverse olivaceous grey, with buff margin; on PDA surface dull green with margin buff, reverse buff; on OA surface dull green with margin buff. Notes -Nothophaeomoniella is related to 'Phaeomoniella' pinifoliorum, which also represents a genus distinct from Phaeomoniella, typified by P. chlamydospora (Crous & Gams  2000, Crous et al. 2015a). A new genus is thus introduced to accommodate this species, isolated from leaves of Ekebergia pterophylla, and forming a coelomycetous morph in culture. The Phaeomoniellales was recently treated by Kraus et al. (2020). Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Vredendaliella oleae (as Phaeomoniellales sp.
CS-2020a; strain CBS 146757, GenBank MT791073. 1; Identities = 471/498 (95 %), ten gaps (2 %)), Aequabiliella effusa (strain P6, GenBank MK801313. 1; Identities = 450/482 (93 %), ten gaps (2 %)) and Aequabiliella palatina (strain JKI-Ap36, GenBank MH999506. 1; Identities = 445/482 (92 %), 11 gaps (2 %)). Closest hits using the LSU sequence are Phaeomoniella pinifoliorum (strain CBS 114903, GenBank NG_064185. 1; Identities = 838/882 (95 %), seven gaps (0 %)), Xenocylindrosporium kirstenboschense (strain CBS 125545, GenBank NG_057857. 1; Identities = 813 /862 (94 %), eight gaps (0 %)) and Paraphaeoisaria alabamensis (strain CBS 101. 77A, GenBank MH872801. 1; Identities = 792 /845 (94 %), 13 gaps (1 %)). No significant hits were obtained when the tef1 sequence was used in blastn and megablast searches.
Distant hits obtained using the tub2 sequence had highest similarity to Vredendaliella oleae (strain CBS 146757, GenBank MW017334. 1; Identities = 275/323 (85 %), two gaps (0 %)), Aequabiliella palatina (strain JKI-Ap36, GenBank MK070469. 1; Identities = 247/308 (80 %), 13 gaps (4 %)) and Moristroma palatinum (strain JKI-Au02, GenBank MK070475. 1; Identities = 254/319 (80 %), 16 gaps (5 %)). Crous, sp. nov. Fungal Planet 1225 -13 July 2021 Crous & Roon. -Lath. , sp. nov. Etymology. Name refers to the state of California, USA, where this fungus was collected.
Supplementary material FP1226 Maximum likelihood (ML) tree obtained from the ITS sequence of A. montepascoalis and sequences retrieved from GenBank. Bayesian inference (BI) and Maximum likelihood analyses were performed using MrBayes v. 3. 2 (Ronquist et al. 2012) and PhyML v. 3. 0 (Guindon & Gascuel 2003), respectively. Bayesian posterior probabilities (BYPP) ≥ 0. 9 and bootstrap values for maximum likelihood (MLBS) ≥ 70 % are placed above the branches (MLBS/BYPP). Support values lower than 0. 9 and 70 % are marked with '*', and absent are marked with '-'. The bar indicates the expected number of substitutions per position. Ex-type, ex-isotype, and ex-neotype strains are marked with superscript ' T ', ' IT ' and ' NT ', respectively. Cunninghamella phaeospora CBS 692. 68 and C. vesiculosa CBS 989.
Colonies on malt extract agar (MEA) 35-38 mm diam, with pinkish white aerial mycelium, wine red exudates diffusing into the medium, reverse dark ruby; ascomata not formed after 7 d. Colonies on PCA at 37 °C after 7 d 18 -20 mm diam. Typus. cZech republic, Brno, tea bag with fruit tea (lemons, rose hips and apple pulp), Nov. 2001, V. Ostrý (holotype PRM 954081, isotype PRC 295, culture ex˗type CCF 3252 = CBS 147158, ITS, LSU, rpb2 and tub2 sequences GenBank MW798185, MW798181, MW816124 and MW816125, MycoBank MB 839209). Notes -Arcopilus navicularis shares an identical ITS and LSU region with A. aureus, A. amazonicus and A. globulus. BLAST analysis with the rpb2 sequences showed 96 -96. 5 % similarity with the latter three mentioned species, while the tub2 gene showed only 87-87. 5 % similarity.
ITS + LSU + rpb2 + tub2 A best scoring maximum likelihood tree based on the ITS, LSU, rpb2 and tub2 genes shows the relationships of A. navicularis with other Arcopilus species. The dataset contained 15 taxa and a total of 2 054 characters of which 421 were variable and 297 parsimony-informative. Partitioning scheme and substitution models for analyses were as follows: the HKY+I model was proposed for the ITS region; TrNef model for the LSU region; TrN+G model for the RPB2 gene; and K80+I for the tub2 gene. The tree was constructed with IQ-TREE v. 1. 4. 4 (Nguyen et al. 2015). Support values at branches were obtained from 1 000 bootstrap replicates; only bootstrap support values ≥ 70 % are shown; ex-type strains are indicated by the superscript  T. The tree is rooted with Achaetomium macrosporum.
coSta rica, from an unidentified fern (Pteridophyta), unknown collection date and collector (deposited in CBS collection Feb. 1999 by P. Lafeber), (holotype CBS H-20817, culture ex-type CBS 101611, LSU sequence GenBank MW724319, other sequences from Damm et al. 2012a,  MycoBank MB 839338). Notes -Based on blastn searches on NCBI GenBank, the ITS sequence of strain CBS 101611 is identical with that of C. scovillei, including its ex-type strain and with strain CBS 129820 from Passiflora in Colombia (Damm et al. 2012a), but also with strains from fronds of Rumohra adiantiformis (leather leaf fern) with anthracnose from Costa Rica (Schiller  et al. 2006) and Florida, USA (MacKenzie et al. 2009). Phylo-genies inferred from concatenated ITS, chs-1, tub2, act, his3 and gapdh sequences placed the fungus in clade 1 of the C.
abscissum, including its ex-type (Crous  et al. 2015a)  Notes -The genus Comoclathris was described by Clements (1909). In 2015 the genus was placed in the Pleosporales by Ariyawansa et al. (2015). Currently, the genus consists of 41 registered names in MycoBank. However, data on only 10 species are represented in GenBank. The type species of the genus is C. lanata, which lacks ITS nrDNA sequence data. Culture characteristics -Colonies on potato dextrose agar flat, slow growing; surface glistering without aerial hyphae; margins irregular; raw umber and orange-citrine at the origin with white spots and lemon chrome margin indicating growing zone; 1. 9 cm diam after 2 wk dark incubation at 23 °C. Typus. auStralia, Queensland, Baxter's Hill, Gowrie Junction, remnant dry rainforest, S27°29'7" E151°52'41", altitude 570 m, isolated as an endophyte from healthy leaves of Geijera salicifolia (Rutaceae), 20 Aug.
2019, A. B. Sharma (holotype BRIP 71282a, ITS and LSU sequences GenBank MT573531 and MT525302, MycoBank MB 836228). Notes -Members of Coniochaeta are characterised by darkbrown to black, globose or subglobose perithecia, longitudinal germ slits in dark ascospores and a phialidic asexual morph (Nasr et al. 2018, Harrington et al. 2019). Coniochaeta spp. occur as endophytes and human and plant pathogens (Damm  et al. 2010, Khan et al. 2013, Xie et al. 2015). A number of bioactive compounds have been isolated from members of the genus including coniochaetone and coniosetin (Wang et al. 1995, Segeth et al. 2003). Etymology. Named after J. M. Bonache, a dear friend, and after a lagoon of the same name, where this species was collected. Classification -Cortinariaceae, Cortinariales, Agaricomycetes.
Hymenophoral trama consisting of 3 -10 µm wide cylindrical hyphae, with greyish parietal pigment and sometimes with vacuolar pigmentation. Basidia claviform, tetrasporic, 25 -35 × 8-10 µm, with stigmata 2-4 µm long. Pileipellis consisting of a duplex cutis (Type 1 according to Bidaud et al. 2004). Epicutis formed by a thin layer of radial hyphae of (3 -)4 -8(-14) µm, with few free and hardly differentiated tips, and greyish brown parietal incrusted pigment. Subcutis well differentiated from the epicutis, consisting of a layer of subcellular elements of 17-23 µm diam, with brownish parietal pigmentation. Clamp connections present in all tissues. Habitat & Distribution -Gregarius and caespitose, in large groups among leaf litter of Quercus ilex subsp. Phylogeny -The final alignment included 663 bp of which 153 were variable and 108 were parsimoniously informative among 31 sequences (three newly generated, 28 retrieved from public databases).
s. l. , in seminatural grassland pasture, 3 Sept. 2019, J. B. Jordal, JBJ19-013 (holotype OF-258322, isotype GB-0207610, ITS-LSU sequence GenBank MW714630, MycoBank MB 839261). Notes -Cuphophyllus flavipesoides belongs in a complex of closely related and morphologically similar species. In macroand micromorphology it is very similar to C. flavipes. On average we find that the spores in C. flavipes are more subglobose with the average measurements 7. 1 × 5. 6 µm, and Q = 1. 22-1. 27, compared to the average in C. flavipesoides, 7. 2 × 5. 3 µm, Q = 1. 34-1. 39. In Voitk et al. (2020) an average value for the spores of the selected epitype of C. flavipes was measured to Q = 1. 2. The two species differ in ITS1 sequence data by four substitutions and four single bp insertion/deletion events, in the ITS2 by six substitutions and three single bp insertion/deletion events.
org, Kurtzman  & Robnett 2003). One of these, E. coryli, has been associated with fruit dry rot in cultivated and indigenous Citrus in Australia (Shivas et al. 2005). During that study, Eremothecium peggii was isolated from fruit with dry rot of Citrus australis (Dooja, round lime), which is native to subtropical rainforest in New South Wales and Queensland, Australia. All species of Eremothecium are considered as plant pathogens, although the pathogenicity of E. peggii as the cause of fruit dry rot has not been demonstrated. Based on a BLASTn search of NCBI GenBank nucleotide database, the closest hit against LSU sequences ex-type specimens were E. cymbalariae (strain NRRL Y-17582, GenBank NR_042628. 1, Identities 854/884 (97 %), Gaps = 4 (0 %)); E. sinecaudum (strain ATCC 58844, GenBank XR_001930265.
1, Identities 867/898 (97 %), Gaps = 5/898 (0 %)); and E. gossypii (strain CBS 109. 26, GenBank NG_063967. 1, Identities 863/899 (96 %), Gaps = 5/899 (0 %)). The closest hit against ITS sequences ex-type specimens was Kluyveromyces aestuarii (ex-type strain CBS 4438; GenBank NR_165976. 1; Identities 208/218 (95 %), Gaps = 1 (0 %)). Culture characteristics -Colonies on malt extract agar and potato dextrose agar umbonate on surface, black, dry to moist, margin undulate, reaching 8 -15 mm diam after 10 d at 20 °C, reverse purplish black without diffusible pigment. Habitat & Distribution -Isolated from surface sterilised roots of Spartina alterniflora from saltwater marsh. Known only from Virginia, USA. Additional material examined. uSa, Virginia, Chesapeake County, from sterilized S. alterniflora root tissue in saltwater marsh, 36.
7928, -76. 2898, c. 1 m a. s. l. , 13 Nov. 2019, D. Raudabaugh, B4-7-NF (ILLS00121430, ITS sequence GenBank MW465990). Notes -Exophiala spartinae was isolated using nitrogen free medium (Kurtzman et al. 2011) from two separate S. alterniflora plants. Phylogenetic analyses of ITS nrDNA sequences employing Maximum Likelihood and Bayesian criteria and implementing Gblocks suggest that the closest taxa to E. spartinae are E. oligosperma, E. jenselmei, E. spinifera, E. exophialae, and Rhinocladiella basitona. This species is distinguished by its subglobose to broadly ellipsoidal conidiophore cells, which consist of one to three cells with a terminal conidiogenous cell. Exophiala spinifera (NR111131) was the closest nrITS BLASTn match (90 %) against the entire E. spartinae ITS sequence. Mycelium composed of hyaline, subhyaline to pale brown, branched, septate hyphae, 2 -3.
, they group distant from Devriesia s. str. in Teratosphaeriaceae represented by D. staurophora, the generic type (Meng et al. 2017). Interestingly, two other strains also named 'Devriesia' sp. , NG_p52, isolated during a study of gene expression of fungal nitrate reductases in agricultural soils from Austria (Gorfer et al. 2011); and MI63, isolated from soil in Germany, grouped within the Extremopsis clade and seem conspecific with E. radicicola, expanding the known distribution of the genus from southern Spain to other localities across Europe. Based on a megablast search of NCBIs GenBank nucleotide data base, the closest hits of Extremopsis radicicola (CBS 147117) using the ITS sequence are 'Devriesia' sp. NG_p52 (GenBank HQ115717. 1; Identities = 232/232 (100 %), no gaps), 'Devriesia' sp.