, (2020) Cat #Ab 259894 N/A Rabbit monoclonal anti-SFPQ ATA GTT AAT AGC GT Abcam Cat #Ab 177149 Bacterial and virus strains E_Sarbeco Reverse: ATA TTG CAG CAG Corman et al. , (2020) N/A SARS-CoV-2 isolate 220_95 TAC GCA CAC A Isolated from patient EPI_ISL_469284 EWSR1 HA Forward: TAA ACT ATG AAT TCA (Onodi et al. , 2021) This paper N/A Chemicals, peptides, and recombinant proteins GGA TGG CGT CCA CGG ATT ACA GTA CCT 32% Paraformaldehyde (formaldehyde) aqueous solution ATA GCC AAG CTG CAG CGC AGC AGG GC Electron Microscopy Sciences Cat #15714 DMSO Triton X-100 GGT ATC CTC CAG CGG CCG AGT AGG GCC CTC AAG CGT AAT CTG GAA CAT CGT ATG EWSR1 HA Reverse: ATA GTT TAG CGG CCG Sigma-Aldrich Sigma-Aldrich This paper Cat #T9284 Cat #D5879 N/A Artemisinin GAT CTC TGC GC Sigma-Aldrich Cat #361593 (S)-(+)-Campthotecin ACE2 Forward: CCG CTC GAG CGG GCC Sigma-Aldrich This paper Cat #C9911 N/A Cerulenin ATG TCA AGC TCT TCC TGG CTC Sigma-Aldrich Cat #C2389 Dabigatran ACE2 Reverse: ATA AGA ATG CGG CCG CTT Sigma-Aldrich This paper Cat #SML2370 N/A Geldanamycin from Streptomyces hygroscopicus AAA AGG AGG TCT GAA CAT CAT CAG TG Sigma-Aldrich Cat #G3381 Phenethyl isothiocyanate ON-TARGETplus Human ILF3 siRNA -SMARTpool Sigma-Aldrich Horizon Discovery Cat #253731 Cat #L-012442-00 Remdesivir ON-TARGETplus Human HNRNPA2B1 siRNA -SMARTpool Selleckchem Horizon Discovery Cat #SE-S8932 Cat #L-011690-01 Sunitinib malate ON-TARGETplus Human QKI siRNA -SMARTpool Sigma-Aldrich Horizon Discovery Cat #PZ0012 Cat #L-024905-01 MyOne C1 beads ON-TARGETplus Human SFPQ siRNA -SMARTpool Thermo Fisher Scientific Horizon Discovery Cat #65001 Cat #L-006455-00 50 mM D-biotin ON-TARGETplus Human CTSL siRNA SMART Pool Thermo Fisher Scientific Horizon Discovery Cat #B20656 Cat #L-005841-00 SUPERase-in ON-TARGETplus Non-targeting Pool Thermo Fisher Scientific Horizon Discovery Cat #AM2696 Cat #D-001810-10-05 N-Laurosylsarcosine sodium salt GGATCTTCAACCACCTCGAG Flow cytometry analysis of ACE2 cell surface expres-Sigma Merck NA-Deoxycholate Sigma Merck QKI sgRNA targeting sequence: This paper Cat #L7414 Cat #30970 N/A sion EDTA ULTROL grade Recombinant DNA B Comprehensive identification of RNA binding proteins Sigma Merck Trichloroacetic acid Sigma Merck pLVX-EF1alpha-IRES-Puro Takara Bio by mass spectrometry B High-confidence RNA binding protein scoring Proteinase K Qiagen pLVX-ACE2 This manuscript Cat #324504 Cat #T0699 Cat #631988 Cat #19133 N/A B Identification of known interactors of coronaviruses in TRizol LS Reagent Thermo Fisher Scientific MGC Human EWSR1 Sequence-Verified cDNA Horizon Discovery Cat #10296028 Cat #MHS6278-202759357 the ChIRP dataset Dynabeads Protein G 3xFlag SFPQ_pUC57-mini Plasmid Thermo Fisher Scientific Genscript Cat #10004D N/A B siRNA screen assay 3XFLAG peptide 3xFlag QKI_pUC57 Plasmid Sigma Merck Genscript Cat #F4799 N/A HA peptide 3xFlag ILF3_pUC57-Mini Plasmid Sigma Merck Genscript Cat #11666975001 N/A GlycoBlue Coprecipitant 3xFlag HNRNPA2B1_pUC57 Plasmid Thermo Fisher Scientific Genscript Cat #AM9515 N/A Lipofectamine RNAiMax Transfection Reagent 3xFlag HSPB1_pUC57 Plasmid Thermo Fisher Scientific Genscript Cat #13778150 N/A Lipofectamine 3000 Transfection Reagent 3xFlag GFP_pUC57 Plasmid Thermo Fisher Scientific Genscript Cat #L3000015 N/A Bolt 4-12% Bis-Tris plus 3xFlag TRIM28_pUC57-mini Plasmid Thermo Fisher Scientific Genscript Cat #NW04122BOX N/A d RESOURCE AVAILABILITY B Lead contact B Materials availability B Data and code availability d EXPERIMENTAL MODEL AND SUBJECT DETAILS B Cell lines d METHOD DETAILS B Virus preparation and titration B Generation of cell lines stably expressing ACE2 B (Continued on next page) e1 Cell Reports 39, 110744, April 26, 2022 (Continued on next page) Cell Reports 39, 110744, April 26, 2022 e2.