*,1 , Hyun-Dong Chang *,2 , Andreas Radbruch *,2 , Andreas Acs 3 , Dieter Adam 4 , Sabine Adam-Klages 13 , William W. Agace5,6 , Nima Aghaeepour 323 , Mübeccel Akdis 7 , Matthieu Allez 8 , Larissa Nogueira Almeida9 , Giorgia Alvisi 10 , GrahamAnderson 11 , Immanuel Andrä 12 , Francesco Annunziato70 , Achille Anselmo65 , Petra Bacher 4,14 , Cosima T. Baldari 15 , Sudipto Bari 319,320 , Vincenzo AO CFSE DNR GATA3 ITIM MIFloCyt PBS SI t-SNE acridine orange carboxyfluorescein succinimidyl ester dynamic detection range GATA binding protein-3 immune-receptor tyrosine-based inhibition motif minimum information about a flow cytometry experiment phosphate buffered saline small intestinal t-distributed stochastic neighbor embedding APC CFU-F DPBS GFP KIRs mitoPY PD-1 SIP TUNEL allophycocyanin colony-forming units-fibroblasts Dulbecco's phosphate buffered saline green fluorescent protein killer Ig-like receptors mitochondria peroxy yellow-1 programmed cell death protein 1 sample injection port TdT-mediated dUTP nick end labelling APD CIMT DPEC GUIs KLH MitoSOX PDGFR SLE VA avalanche photodiodes cancer immunotherapy consortium double positive effector cells graphical user interfaces keyhole limpet hemocyanin mitochondrial superoxide indicator platelet-derived growth factor receptor systemic lupus erythematosus voltage APS CIP E:T GZMB KLRG1 MMM PEA SLEC ULK1 ammonium peroxodisulfate CIMT immunoguiding program effector-to-target cell granzyme B killer cell lectin-like receptor G1 metal-minusmany phosphatidylethanolamine short-lived effector cells Serine/threonine-protein kinase ULK1 ASCs CK ECI 2015 HBSS K-S MN PE SLOs UV antibody-secreting cells cytokeratin 18 European Congress of Immunology ECI 2015 Hank's balanced saline solution Kolmogorov-Smirnov statistic monensin phycoerythrin secondary lymphoid organs ultraviolet ATCC CM EdU HCC L/D MoDC PerCP SMO Var American type tissue collection central memory 5-ethynyl-2-deoxyuridine hepatocellular carcinoma live dead monocyte-derived DC peridinin-chlorophyll signal-minus-one variance ATGs cMOP EGFR HCS LCA MPEC PI SNRs VLR autophagy related genes common monocyte precursors epidermal growth factor receptor hematopoietic progenitor cells leukocyte common antigen memoryprecursor effector cells propidium iodide signal-tonoise ratios variable lymphocyte receptor AxA5 CMV EM HHV8 Lin mRNA PMA SOCE Vps34 fluorophore-conjugated annexin A5 cytomegalovirus effector memory cells human herpesvirus 8 lineage markers messenger RNA phorbol 12-myristate 13-acetate store-operated Ca2+ entry vacuolar protein sorting34 BafA CS&T ENOBs HIPAA Lin-MSPCs PMT SOV WDM bafilomycin A1 cytometer setup and tracking effective number of bits Health Insurance Portability and Accountability Act lineage-negative mesenchymal stem and progenitor cells photomultiplier tube spillover value wavelength division multiplexer BAFF CSF ERK pathway HIPC lncRNA mt PP SP Bcell activating factor cerebrospinal fluid extracellular-signal regulated kinase pathway Human Immunology Project Consortium long non-coding RNA mitochondrial phagocytosis product short pass BCL6 CT ESCCA HIV LmP mtmP PS SPADE B-cell lymphoma 6 cancer-testis European Society for Clinical Cell Analysis Human immunodeficiency virus lamina propria mitochondrial membrane potential phosphatidylserine Spanning-tree progression analysis of densitynormalized BD CTCs FACS HLA LP mTOR PVP Becton Dickinson circulating tumor cells fluorescence activated cell sorting human leukocyte antigen long pass mammalian target of rapamycin complex 1 polyvinylpyrrolidone data bDNA CTL FBS HSC LPS NAO PY(G) β2m branched DNA technology cytolytic cells fetal bovine serum hematopoietic stem cell lipopolysaccharide nonyl acridine orange pyronin Y(G) beta-2-microglobulin BDS CV Fc HTS LRR NIAID RAR SSC bright detail similarity coefficient of variation fragment of immunoglobulins high throughput system leucine-rich repeat National Institute of Allergy and Infectious Diseases retinoic acid receptor side scatter BF CW FCCP IBD LRR-CT NIH RCC SSM brightfield continuous wave carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone inflammatory bowel disease LRR C-terminal capping National Institutes of Health renal cell carcinoma spillover-spreading matrix BFA Cy FCM ICCS LRR-NT NIR Rho123 TAA brefeldin A cyanine flow cytometry International Clinical Cytometry Society LRR N-terminal near infrared rhodamine-123 tumor-associates antigens BISC CyTOF FCS ICPC TOF-MS LTi NK RNase Tcon Bioinformatics Integration Support Contract Cytometry by Time-Of-Flight fetal calf serum inductively-coupled plasma time of flight mass lymphoid tissue-inducer cells natural killer ribonuclease conventional T cells BM DAIT FCS mAb NLOs ROR-γt TCR bone marrow Division of Allergy, Immunology, and Transplantation flow cytometry standard spectrometry monoclonal antibody non-lymphoid organs retinoic acid receptor-related orphan receptor gamma T cell receptor BP DAMP FDA IdU MAP kinase NSCLC RORγtfm TCRtg band pass dangerassociated molecular patterns fluorescein-diacetate Iodo-2-deoxyuridine membrane activated protein kinase non-small cell lung cancer RORγt fate mapping TCR-transgenic BrdU DAPI FGFR IFN MARS-seq NYSTEM Program ROS TdT 5-bromo-2-deoxyuridine 4,6-diamidine-2-phenylindole fibroblast growth factor receptor interferon massive parallel single-cell RNA-seq New York State Department of Health Program reactive oxygen species terminal deoxynucleotidyltransferase BSA DCF FISH Ig mBCs OH RPMI 1640 Th cells bovine serum albumin 2,7-dichlorofluorescein fluorescent in situ hybridization immunoglobulin memory B cells hydroxyl radicals Roswell Park Memorial Institute 1640 T helper cells BV DCFDA FITC IL MCs OMIP RT TIA brilliant violet 2-7-dichlorodihydrofluorescein diacetate fluorescein isothiocyanate interleukin mononuclear cells optimized multicolor immunofluorescence panels room temperature transimpedance CCCP DCs FL1-10 ILCs MdFI OVA SAPK/JNK pathway TM carbonyl cyanide 3-chloro phenyl hydrazine dendritic cells • DCV: dyecycle violet fluorescence channels innate lymphoid cells median fluorescence intensity ovalbumin stress-activated protein kinase/c-jun n-terminal kinase memory T lymphocytes CCDs DHR FLIMs ImmPort MDP OxPhos SAW TMRE charge-coupled devices dihydrorhodamine fluorescent life time measurements immunology database and analysis portal monocyte/macrophage-DC precursors oxidative phosphorylation surface acoustic waves tetramethylrhodamine CLL DIOC6 FMK iono MFI PI3K SB TNF chronic lymphocytic leukemia 3,3-dihexyloxacarbocyanine iodide fluoromethyl ketone ionomycin mean fluorescence intensity Phosphatidylinositol-4,5-bisphosphate 3-kinase staining buffer tumor necrosis factor cDCs DLD FMO IRF4 MHC PAMPs SD TOF classical DCs deterministic lateral displacement fluorescence minus one interferon regulatory factor 4 major histocompatibility complex pathogen-associated molecular patterns standard deviation time-of-flight CDR DLP FOCIS ISAC MIBBI PB SE Treg complementarity determining regions dichroic longpass Federation of Clinical Immunology Societies International Society for Analytical Cytology minimum information for biological and biomedical peripheral blood standard error regulatory T cells CEA DMSO FOXP3 ISR PBMC SEB TRAIL carcinoembryonal dimethyl sulfoxide forkhead box P3 internal store release investigations peripheral blood mononuclear cells Staphylococcus enterotoxin B TNF-related apoptosis-inducing ligand CECs DNase FSC ITAM MIBI PBPC SERS TSLP circulating endothelial cells deoxyribonuclease forward scatter immune-receptor tyrosinebased activating motifs multiplexed ion beam imaging peripheral blood progenitor cell surface enhanced Raman scattering thymic stromal lymphopoietin.