dataesr/scientific-paragraphs-categorization huggingface dataset:
A. bohemica A 50 % majority consensus rule maximum likelihood tree based on ITS and LSU rDNA sequences showing the relationships of taxa within the genus Acidiella. Partitioning scheme and substitution models for analyses were selected using Parti-tionFinder v. 1. 1. 1 (Lanfear et al. 2012 ): the HKY+I model was proposed for the ITS1 + ITS2 region, and a K80 model for the 5. 8S + LSU region. The tree was constructed with IQ-TREE v. 1. 4. 0  (Nguyen et al. 2015). The dataset contained 13 taxa and a total of 813 characters of which 102 were variable and 23 parsimony-informative. Support values at branches were obtained from 500 bootstrap replicates. Classification -Psathyrellaceae, Agaricales, Agaricomycetes. Cap 12 -50 mm broad, 10 -30 mm high, convex to conical convex, with prominent umbo, glabrous, sometimes somewhat wrinkled, orange brown when young, then dark beige brown or date colour, hygrophanous, after drying it becomes first pale greyish beige to ochraceous beige, then greyish white.
2-0. 4 mm between the striae. Perceptible bright contrasting with the exoperidium. Stipe 1 mm long, greyish brown (7F3). Epiphragm not observed. Peridioles brownish grey (7F2) to black, 1. 5-1. 75 × 1. 2-1. 5 mm, in number of 6 (average) in each basidiomata, circular to irregular in shape, smooth surface, tunic indistinct and provided with double layered cortex. Basidiospores smooth, hyaline, 32. 5 -47 × 22. 5 -28. 5 µm (L = 38. 9 µm; W = 25. 9 µm; n = 30 spores), slightly elliptical to elongated (Q = 1. 25-1. 79), elliptical in average (Q m = 1. 51), apicule absent and spore wall 2. 3-4. 9 µm in thickness. Typus. BraZil, Rio Grande do Norte, Natal, Pitimbu, on decaying wood, 12 Feb. 2013, A. S. Medeiros (holotype UFRN-Fungos 2798, ITS and LSU sequences GenBank KX966026 and KX966027, MycoBank MB818580).
Branches corresponding to partitions reproduced in less than 50 % bootstrap replicates are collapsed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There was a total of 432 positions in the final dataset. Phylogenetic analyses were conducted in MEGA v. 4. Ganoderma mizoramense Zothanzama, Blanchette, Held, C.
cyanescens, G. lacteus, G. pseudolacteus, G. ammophilus and G. castaneus. The closest species to G. pseudocyanescens is G. cyanescens, which should be considered a complex of cryptic species  (Vizzini et al. 2015). These authors typified G. cyanescens by selecting Bulliard's plate 369  (Bulliard 1788)  as a lectotype (iconotype) and a collection from Italy under Pinus sylvestris as an epitype. Sequences of G. cyanescens have been deposited in GenBank. Gyroporus pseudocyanescens and G. cyanescens seem to be sibling species which are difficult to separate only based on morphology. Gyroporus lacteus differs from G. pseudocyanescens by its whitish pileus covered by large and irregular scales, and by fruiting in Mediterranean woods with Pinus pinea and Quercus ilex. Gyroporus pseudolacteus differs from G.
Clamp connections not observed on any hyphae. Habit, Habitat & Distribution -In small groups, on humusrich soil. Known only from the type locality in Kerala State, India. Typus. india, Kerala State, Wayanad District, Tirunelli, Brahmagiri Hill, from a shola forest of rolling shola grasslands of Western Ghats, 17 Nov. 2010, K. N. Anil Raj (holotype CAL 1526, ITS and LSU sequences GenBank KY807130 and GenBank KY815097, MycoBank MB820656). Notes -The combination of characters such as the hymeniderm-type pileipellis composed of clavate or inflated-clavate terminal elements and the absence of clamp connections indicates that this species belongs to the genus Hodophilus  (Adamčík et al. 2016 , Birkebak et al. 2016). Hodophilus hymenocephalus, a species originally described from USA by  Smith & Hesler (1942, as Hygrophorus hymenocephalus) , shows similarity with H.
indicus in having a similar-looking pileus with somewhat similar surface features, almost similar number and attachment of lamellae, similar-sized basidiospores (4-5 µm), an irregular lamellar trama and a similar pileipellis. Hodophilus hymenocephalus, however, is distinguished by its pale pinkish cinnamon to brown pileus, hair-brown lamellae, longer stipe (3-4 cm), a hymenium devoid of marginal cells and the geographical location. Additionally, a pairwise comparison of the ITS sequences (GenBank KY807130/DQ484066) of these two species showed only 87 % sequence similarity (with a high e-value). Hodophilus micacea shares a few features with H. indicus such as a hygrophanous pileus with somewhat similar surface features, rather similarly-attached lamellae, somewhat similar-sized basidiospores ((3.
A BLASTn search using the ITS (593 bp) sequence of H. indicus showed H. micaceus (GenBank KU882873; 91 % identity) as the closest hit. While using the LSU (706 bp) sequence, Hodophilus micaceus (GenBank KP257222; 93 % identity), a collection from Slovakia resulted as the closest hit. ML and BI analyses of the combined ITS and LSU dataset recovered two large clades designated as Hodophilus micaceus and Hodophilus foetens superclades following  Adamčík et al. (2016). Hodophilus indicus was found nested inside the Hodophilus micaceus superclade with strong posterior probability (0. 98 PP) and weak bootstrap support (58 % BS). Within this Hodophilus micaceus superclade, H. indicus resolved as an independent lineage well-differentiated from other species of the clade with significant support values (0.
Typus. ecuador, Morona Santiago province, Sangay National Park, alt. 1 510 m, Jan. 2015, C. Vivanco (holotype QCAM4254, ITS-LSU sequence GenBank KY582489, MycoBank MB819814). Notes -According to the description of  Pegler & Fiard (1978) , H. sangayensis belongs to the section Firmae, with H. occidentalis as the closest species based on morphological characters. However, it differs by having a scaly pilial surface, non-glabrous, non-translucent, non-striated, with the lamellae margin non-heterogeneous. The complete ITS sequence of the H. sangayensis holotype was used for the BLASTn search. The results gave the highest score to a Hygrocybe sp. (GMB-2014, GenBank KP012900) from Australia, but with only 44 % coverage and 87 % identity. The top seven BLASTn hit species with full ITS sequences were downloaded for the phylogenetic analysis.
2015, A. Salazar (holotype QCAM4359, ITS-LSU sequence GenBank KY582490, MycoBank MB819896). Notes -According to the description of  Pegler & Fiard (1978) , H. macrosiparia belongs to the section Firmae. The closest species based on morphological characters is H. siparia. However, it differs by having a flat and non-umbilicate pileus which exceeds in 5 mm the maximum size reported, and an orange yellowish colour instead of crimson. The complete 578 bases of ITS sequence of the H. macrosiparia holotype was used for the BLASTn search. The results gave the highest score to a H. occidentalis (PR-6493, GenBank EU435151) from Puerto Rico, but with only 63 % coverage and 87 % identity. The top seven BLASTn hit species were downloaded for phylogenetic analysis. There were significant indels among the aligned sequences.
Growth rates at different temperatures on CMA/PDA/YM (in mm): 10 °C 12-14/  14-18/10-17; 15 °C 31-34/29 -35/23 -29; 20 °C 45 -48/40 -45/25 -36; 25 °C 39 -48/40 -46/35 -55; 30 °C 10 -16/10 -16 /8 -18; 37 °C no growth. Typus. cZech repuBlic, Bohemian Karst, Malá Amerika mine, on bat droppings of  Myotis myotis, 21 Feb. 2009, coll. P. Špryňar, isol. A. Kubátová (holotype PRM 935803, isotypes PRM 935804, PRM 935805, PRC 3709 , culture ex-type CCF 5407 = CBS 141864; ITS, LSU, and SSU sequences GenBank LT627243, LT627240, and LT671443, MycoBank MB819230). Additional material examined. cZech repuBlic, Bohemian Karst, Malá Amerika mine, sediment, 2 Mar. 2013, coll. A. Kubátová, isol. A. Kubátová using hair baiting technique (culture CCF 5406 = CBS 141863, herbarium specimen PRC 3708, ITS sequence GenBank LT627244).
Notes -CCF 5407 has an identical ITS sequence to that of CCF 5406. Based on ITS sequences, M. cremea is 99 % (486/492) similar to strain UAMH 3124 (GenBank KF477240) isolated from a reptile during the study of  Sigler et al. (2013) ; the similarity of the other sequences deposited in GenBank did not exceed 87 %. The LSU rDNA sequence exhibited the highest similarity (95 %) to various species of Arthroderma, Microsporum and Onygena. Sigler et al. (2013)  investigated taxonomic position of the strain UAMH 3124 and classified it as an undetermined fungus at generic as well as species level, which belonged to the phylogenetic lineage of Arachnotheca glomerata. We used the LSU sequence dataset of onygenalean fungi published by  Hirooka et al. (2016)  to assess the phylogenetic position of M. cremea (data not shown).
Conidiogenous cells integrated, terminal or intercalary, polyblastic, sympodial, with several denticle-like loci. Conidia solitary, 1-celled or septate, obpyriform or rostrate, often constricted at the septum, smooth-walled or echinulate, brownish, subhyaline towards the apex, often with a protuberant hilum. Sexual morph unknown. Type species. Neodactylaria obpyriformis Guevara-Suarez, Deanna A. On PCA and OA colonies flat, floccose at the centre, cottony towards the periphery, olive grey (3F2), margin smooth and entire; reverse grey (3E1); sporulation abundant. On PDA flat, white to cream-coloured, margin entire; sporulation sparse. The fungus does not grow at 37 °C. Typus. USA, Arizona, Phoenix, from human bronchoalveolar lavage, D. A. Sutton, 2015 (holotype CBS H-23131 , cultures ex-type UTHSCSA DI 15-121 = FMR 14604; ITS and LSU sequences GenBank LT839090 and LT839091, MycoBank MB820858).
2004 ). Physiological profiles further support the separation of the new species as distinct from C. tanzawaensis and C. panamericana. The new species can be distinguished from C. tanzawaensis by its ability to grow in 50 % glucose. The new species can be distinguished from C. panamericana by its ability to assimilate arbutin and its inability to ferment either D-xylose or galactose. The new species can be distinguished both from C. tanzawaensis and C. panamericana by its inability to grow at 30 °C. All supplementary data including assimilation tests and sequence alignments are available at doi:10. 7931/J2XW4GQT, specimen and strain data is available at https://scd. landcareresearch. co. nz. Etymology. The specific epithet 'vespimorsuum', referring to 'wasp stings'. Five students from the class of 32 at Karamu High School, Hastings, New Zealand who discovered this new species and chose the name were stung by invasive wasps during the fungal collecting trip.
Hence 'the Candida of the wasp-stings'. Classification -Incertae sedis, Saccharomycetales, Saccharomycetes, Saccharomycotina. On Yeast extract Malt agar (YM), after 9 d at 22 °C, colony is white, moist and glistening, with a somewhat raised, lobed, membranous margin. After 5 d growth at 22 °C in YM broth, cells are subglobose to globose, ellipsoidal and cylindrical, (3-)4. 5 -7. 5(-8) × (2. 5 -)3. 5 -6(-7. 5) µm (av. 5. 6 × 4. 8 µm), occurring singly, in clusters or chains, as pseudohyphae, and proliferating by budding. Dalmau plate culture after 10 d was white with an undulating to entire margin. Fermentation and assimilation of carbon compounds -see MycoBank MB819395. Typus. new Zealand, Hawke's Bay Region, White Pine Bush Scenic Reserve, on cup fungus surface, 2 Mar. 2016 (holotype PDD 105304, culture ex-type = ICMP 22109, ITS and LSU sequences GenBank KY285004 and KY285007, MycoBank MB819395).
7931/J2XW4GQT, specimen and strain data are available at https://scd. landcareresearch. co. nz. Fungal Planet 610 -20 June 2017  Guevara-Suarez, Cano-Canals, Cano & Stchigel, sp. nov. Etymology. From Latin parvum-, small, and -fructum, fruit, in reference to the small size of the conidiophores.
ITS. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence are Penicillium dimorphosporum (GenBank NR 121271; Identities = 534/553 (97 %), Gaps = 4/553 (0 %)), Penicillium erubescens (GenBank NR 121245; Identities = 532/551 (97 %), Gaps = 6/551 (1 %)), and Penicillium rubidurum (GenBank NR 121243; Identities = 531/551 (96 %), Gaps = 5/551 (0 %)). BenA. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the BenA sequence are Penicillium rubidurum (GenBank HQ646574; Identities = 408/466 (88 %), Gaps = 4/466 (0 %)), Penicillium dimorphosporum (GenBank KF900165; Identities = 383/429 (89 %), Gaps = 5/429 (1 %)), and Penicillium pimiteouiense (GenBank KC344994; Identities = 406/467 (87 %), Gaps = 7/467 (1 %)).
CaM. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the CaM sequence are Penicillium dimorphosporum (GenBank KF900176; Identities = 472/544 (87 %), Gaps = 13 /544 (2 %)), Penicillium vinaceum (GenBank AY678543; Identities = 452/544 (83 %), Gaps = 18/544 (3 %)), and Penicillium pimiteouiense (GenBank HQ646580|; Identities = 454/548 (83 %), Gaps = 25/548 (4 %)). Penicillium parvofructum differs from P. dimorphosporum (the species phylogenetically more closely related) in the size of the stipes of the conidiophores (13-18 µm long in P. parvofructum vs 15-30 µm long in P. dimorphosporum), in the morphology of the conidia (P. parvofructum produces hyaline to subhyaline, smooth-walled, broadly ovoid to bacilliform conidia, which turn brown, ornamented and globose with age in P.
Hyphal system dimitic, generative hyphae thin-walled, smooth and with clamps, 2-4 μm wide, skeletal hyphae weakly dextrinoid, 2-3 μm. Cystidia or other sterile elements absent. Basidia 14 -20 × 4-6 μm, clavate with four sterigmata. Basidiospores 3-4 × 2-4 μm, globose to subglobose, hyaline, thick-walled and dextrinoid. Notes -Based on a BLASTn search of NCBIs GenBank database, the closest hits using the ITS sequence are Perenniporia sp. (GenBank KT156689; Identities = 588/598 (98 %), Gaps = 1/598 (0 %)), Dichomitus squalens (GenBank KM411455; Identities = 631/666 (95 %), Gaps = 4/666 (0 %)), and P. tenuis (GenBank JQ001859; Identities = 631/667 (95 %), Gaps = 4/ 667 (0 %)). Using the LSU sequence, the highest similarity was to P. aridula (GenBank JQ001847; Identities = 801/817 (98 %), Gaps = 7/817 (0 %)), P.
aridula (GenBank JQ001846; Identities = 801/817 (98 %), Gaps = 7/817 (0 %)), and P. tibetica (Gen-Bank JF706332; Identities = 801/817 (98 %), Gaps = 7/817 (0 %)). Although genetically close to P. aridula, P. tenuis and P. tibetica, P. brasiliensis is morphologically different (Table  1  -see FP 612). Perenniporia brasiliensis is similar to P. albo-incarnata, P. centrali-africana, and P. guyanensis, sharing the same whitish colour. However, they are micro-morphologically different (Table  1  -see FP 612). Carla R. S. Lira, Adriene M. Soares, Tatiana B. Gibertoni, Departamento de Micologia Prof. Chaves Batista, Universidade Federal de Pernambuco, Recife, Brazil; e-mail: carla-rejane@hotmail. com, adrienemssoares@gmail. com & tbgibertoni@hotmail. com Leif Ryvarden, University of Oslo, Institute of Biological Sciences, P.
Typus. chile, Santiago, isolated from human toe nail, Nov. 2016, C. Tapia (holotype SGO 167659, ex-type culture CCCT 17. 04, ITS and LSU sequences GenBank KY798313 and KY798314, MycoBank MB820647). Notes -This fungus was isolated from toe nail lesions of an immunocompromised patient. The clinical case is currently under study and will be reported elsewhere. The isolate remained sterile or sporulated poorly on routine mycological media, such as malt extract agar or Sabouraud dextrose agar. Therefore, it was grown on PNA in order to stimulate sporulation. On this medium, undifferentiated fertile hyphae produced cladosporioid, coherent chains of aseptate, subglobose to elongate dematiaceous conidia without dark scars. These morphological features closely resembled those of Cladophialophora (Chaetothyriales), a genus which includes important clinically-relevant species with a broad clinical spectrum, including chromoblastomycosis, phaeohyphomycosis, mycetoma and onychomycosis  (Badali et al.
2008 , Brasch et al. 2011). BLAST searches with the ITS sequence of isolate CCCT 17. 04, however, revealed affinities with species of Phialocephala (Helotiales), and the closest match was the type species, P. dimorphospora (ex-type strain, CBS 300. 62, ITS sequence GenBank AF486121, and other strains, 97-98 % identical). Considering that the ITS region provides little resolution for closely related taxa in Phialocephala  (Tanney et al. 2016) , isolate CCCT 17. 04 was considered to represent a species different from P. dimorphospora. The genus Phialocephala traditionally has been characterised by macronematous conidiophores bearing penicillately arranged, phialidic conidiogenous cells with deep collarettes and aseptate conidia in slimy masses  (Kendrick 1961 , Seifert et al. 2011). These structures are produced by P.
ru & Anna. Kiyashko@binran. ru Colour illustrations. Russia, South Siberia, Sayano-Shushenskiy State Biospheric Nature Reserve, site in taiga where fungus was found; basidiocarps, basidiospores, cheilocystidia, caulocystidia (all from holotype). Scale bars = 1 cm (basidiocarps), 10 µm (microscopic structures). Best tree from a ML analysis of the ITS dataset of some Pholiotina species from sect. Piliferae. Support values (BS ≥ 50 % / PP ≥ 0. 95) are given above the branches. Maximum likelihood analysis was run in the PhyML server v. 3. 0 (http://www. atgc-montpellier. fr/phyml/) under a GTR model, with 100 rapid bootstrap replicates. Bayesian analysis was performed with MrBayes v. 3. 1 software  (Ronquist & Huelsenbeck 2003)  for two independent runs, each with 5 million generations and under the same model.
Taxa names are followed by the GenBank accession numbers. The novel species is indicated in bold. The tree was rooted to Conocybe apala (GenBank JX968209). The scale bar indicates the expected changes per site. Fungal Planet 615 -20 June 2017 Phytophthora mekongensis  Cacciola & N. V. Hoa, sp. nov. Etymology. Name refers to the area from where the species was isolated, Mekong River Delta in Vietnam. Classification -Peronosporaceae, Peronosporales, Peronosporomycetes. Sporangia produced on V8-agar (V8A) flooded with both distilled water and non-sterile soil extract  (Jung et al. 2017) , formed in dense sympodia and were limoniform, ovoid-obpyriform, ellipsoid to fusiform, papillate, frequently bi-papillate and bior tri-lobed, often caducous (pedicel length 5 -15 μm) with a conspicuous basal plug at the point where the pedicel attaches to the sporangium; average size of sporangia was 35 × 24 μm (overall range 25-50 × 20-36 μm) with a mean length/breadth ratio of 1.
Among these species in Melastomataceae, Pseudocercospora melastomobia is morphologically similar but distinguishable from P. leandrae-fragilis by having longer and wider conidiophores (10-50 × 3. 5-5. 5 µm) and smaller conidia (50-150 µm). Additionally, P. leandrae-fragilis does not correspond to any sequences available in GenBank at present. Hence, it is described here as a new species.
ITS. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence are Pseudocercospora basitruncata (GenBank KF901632; Identities = 441/446 (99 %), Gaps = 3/446 (0 %)), Pseudocercospora sp. (GenBank DQ303084; Identities = 440/445 (99 %), Gaps = 2/445 (0 %)), and Pseudocercospora paranaensis (GenBank KT037523; Identities = 438/445 (98 %), Gaps = 2/445 (0 %)).
LSU. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the LSU sequence are Pseudocercospora rhabdothamni (GenBank JQ324964; Identities = 849/849 (100 %), no gaps), Pseudocercospora cyathicola (GenBank JF951159; Identities = 849/849 (100 %), no gaps), and Pseudocercospora humuli (GenBank GU214676; Identities = 849 /849 (100 %), no gaps). Picillo & Vizzini, sp. nov. Etymology. The epithet refers to the locality, Monti del Matese, where this species was found.
Based on a megablast search of NCBIs GenBank nucleotide database, the closest hit with the ex-type strain using the ITS sequence is Saksenaea vasiformis PWQ2338 (GenBank KP132601; Identities = 694/739 (94 %), Gaps 27/739 (3 %)); using the LSU sequence it is Saksenaea erythrospora strain UTHSC 06-576 (GenBank HM776683; Identities = 714/735 (97 %), Gaps 3/735 (0 %)); and using the EF1-α sequence it is Saksenaea vasiformis strain FMR 10131 (HM776689; Identities = 465/477 (97 %), no gaps). Our phylogenetic tree, built from the ITS, LSU, and EF1-α nucleotide sequences, corroborated that our isolates represent a new species, the closest species being S. vasiformis, with 93. 6 % similarity with respect to the ex-type strain (NRRL 2443). The sporangiospores of S. loutrophoriformis are similar in size to the S.
2012, M. Staniaszek-Kik (holotype WA 18945, isotype CBS H-22968, ITS and LSU sequences GenBank KU925171 and KU985053, MycoBank MB816126). Notes -The genus Tolypocladium (= Elaphocordyceps) was established in 1971 for three species of soil-isolated fungi and currently is defined mainly on a molecular basis  (Sung et al. 2007). Quandt et al. (2014)  accepted 27 species in the genus and  Gazis et al. (2014)  described three species isolated from Hevea (rubber tree). Diverse ecologies of Tolypocladium taxa (parasites of fungi, insects and rotifers, soil saprobes, plant endophytes) are explained by the 'host habitat hypothesis'  (Nikoh & Fukatsu 2000 , Gazis et al. 2014 ). Only T. inflatum has a known sexual morph. The asexual morph has been also reported for T. japonicum (cultural studies by  Ke & Ju 2015).
ITS. Based on a megablast search against the INSDC (GenBank) nucleotide database, the closest hits using the ITS sequence of type material are several sequences originating from Tuber excavatum groups: GQ217540: Identities 294/344 (85 %), Gaps 8/344 (2 %); FM205567: 306/361 (85 %), Gaps 13/361 (3 %), with less than 70 % query cover. LSU. The closest hits using the LSU sequence of type material are Tuber species from different species groups: KT067698: Identities 512/566 (90 %), Gaps 6/566 (1 %); KT067703: 511/566 (90 %), Gaps 6/566 (1 %).
Typus. new Zealand, Ohau Wines, 2 Bishops Road, RD 20, Ohau 5570, Levin, on Vitis vinifera (Vitaceae), 2014, D. Davis (holotype CBS H-23080, culture ex-type CPC 28701 = CBS 142517 = T14_2612P = ICMP 22045, ITS, LSU, tef1, and tub2 sequences GenBank KY979751, KY979806, KY979891, and KY979918, MycoBank MB820944).
Typus. origin uncertain, intercepted during post entry quarantine in New Zealand, on Rhaphiolepsis indica (Rosaceae), June 2015, M. Toome-Heller (holotype CBS H-23087, culture ex-type CPC 28707 = CBS 142524 = T15_03251A = ICMP 21068, ITS, LSU, rpb2, tef1, and tub2 sequences GenBank KY979758, KY979813, KY979851, KY979896, and KY979924, MycoBank MB820951).
Typus. australia, Western Australia, Denmark, Mount Lindesay Walk Trail, on leaves of Xanthorrhoea sp. (Xanthorrhoeaceae), 19 Sept. 2015, P. W. Crous (holotype CBS H-23093, cultures CPC 29725 = CBS 142530, ITS, LSU, and actA sequences GenBank KY979765, KY979820, and KY979860, MycoBank MB820955); CPC 29547, CPC 29723, ITS, LSU, actA, and tub2 sequences GenBank KY979763 -KY979764, KY979818 -KY979819, KY979858 -KY979859, and KY979929 (CPC 29547).