Title: An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein
Authors: last_name: Dewari
first_name: Pooran Singh
name: Pooran Singh Dewari
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Southgate
first_name: Benjamin
name: Benjamin Southgate
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Mccarten
first_name: Katrina
name: Katrina Mccarten
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Monogarov
first_name: German
name: German Monogarov
affiliations: label: 2
institution: German Cancer Research Center (DKFZ)
country: Germany
name: German Cancer Research Center (DKFZ) , Im Neueheimer Feld 581, 69120, Heidelberg, Germany .
last_name: O’Duibhir
first_name: Eoghan
name: Eoghan O’Duibhir
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Quinn
first_name: Niall
name: Niall Quinn
affiliations: label: 4
institution: Edinburgh Cancer Research UK Centre Institute of Genetics and Molecular Medicine (ECRC-IGMM), University of Edinburgh
country: UK
name: Edinburgh Cancer Research UK Centre Institute of Genetics and Molecular Medicine (ECRC-IGMM), University of Edinburgh , Crewe Road South, Edinburgh, EH4 2XR, UK
last_name: Tyrer
first_name: Ashley
name: Ashley Tyrer
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Plumb
first_name: Colin
name: Colin Plumb
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Blin
first_name: Carla
name: Carla Blin
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Finch
first_name: Rebecca
name: Rebecca Finch
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
orcid: http://orcid.org/0000-0002-5673-9563
last_name: Bressan
first_name: Raul Bardini
name: Raul Bardini Bressan
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Morrison
first_name: Gillian
name: Gillian Morrison
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Jacobi
first_name: Ashley M.
name: Ashley M. Jacobi
affiliations: label: 3
institution: Integrated DNA Technologies, Inc.
country: USA
name: Integrated DNA Technologies, Inc. , 1710 Commercial Park, Coralville, IA, 52241, USA
last_name: Behlke
first_name: Mark A.
name: Mark A. Behlke
affiliations: label: 3
institution: Integrated DNA Technologies, Inc.
country: USA
name: Integrated DNA Technologies, Inc. , 1710 Commercial Park, Coralville, IA, 52241, USA
last_name: Kriegsheim
first_name: Alex von
name: Alex von Kriegsheim
affiliations: label: 4
institution: Edinburgh Cancer Research UK Centre Institute of Genetics and Molecular Medicine (ECRC-IGMM), University of Edinburgh
country: UK
name: Edinburgh Cancer Research UK Centre Institute of Genetics and Molecular Medicine (ECRC-IGMM), University of Edinburgh , Crewe Road South, Edinburgh, EH4 2XR, UK
last_name: Tomlinson
first_name: Simon
name: Simon Tomlinson
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
last_name: Krijgsveld
first_name: Jeroen
name: Jeroen Krijgsveld
affiliations: label: 2
institution: German Cancer Research Center (DKFZ)
country: Germany
name: German Cancer Research Center (DKFZ) , Im Neueheimer Feld 581, 69120, Heidelberg, Germany .
orcid: http://orcid.org/0000-0001-6428-0492
last_name: Pollard
first_name: Steven M.
name: Steven M. Pollard
corresponding: yes
affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
Affiliations: label: 1
institution: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh
country: UK
name: MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, University of Edinburgh , 5 Little France Drive, Edinburgh EH16 4UU, UK .
label: 2
institution: German Cancer Research Center (DKFZ)
country: Germany
name: German Cancer Research Center (DKFZ) , Im Neueheimer Feld 581, 69120, Heidelberg, Germany .
label: 3
institution: Integrated DNA Technologies, Inc.
country: USA
name: Integrated DNA Technologies, Inc. , 1710 Commercial Park, Coralville, IA, 52241, USA
label: 4
institution: Edinburgh Cancer Research UK Centre Institute of Genetics and Molecular Medicine (ECRC-IGMM), University of Edinburgh
country: UK
name: Edinburgh Cancer Research UK Centre Institute of Genetics and Molecular Medicine (ECRC-IGMM), University of Edinburgh , Crewe Road South, Edinburgh, EH4 2XR, UK
Field: Biochemistry, Genetics and Molecular Biology
Document type: preprint
Year: 2018