page_title: Cytochrome P450 aromatic O-demethylase
hierarchical_section_title: Cytochrome P450 aromatic O-demethylase
caption_attribution_description: English: Crystal structure of Amycolatopsis cytochrome P450 (green spheres) GcoA (cartoon diagram) in complex with guaiacol (magenta spheres) based on PDB accession number 5NCB
context_page_description: Cytochrome P450 aromatic O-demethylase is a bacterial enzyme that catalyzes the demethylation of lignin and various lignols. The net reaction follows the following stoichiometry, illustrated with a generic methoxy arene:
  ArOCH₃ + O₂ + 2 e⁻ + 2 H⁺ → ArOH + CH₂O + H₂O
  The enzyme is notable for its promiscuity, it effects the O-demethylation of a range of substrates, including lignin.
  It is a heterodimeric protein derived from the products of two genes. The component proteins are a cytochrome P450 enzyme and a three-domain reductase complexed with three cofactors.
context_section_description: Cytochrome P450 aromatic O-demethylase is a bacterial enzyme that catalyzes the demethylation of lignin and various lignols.  The net reaction follows the following stoichiometry, illustrated with a generic methoxy arene:
  ArOCH₃  +  O₂  + 2 e⁻  +  2 H⁺  →   ArOH  +  CH₂O  +  H₂O
  The enzyme is notable for its promiscuity, it effects the O-demethylation of a range of substrates, including lignin.
  It is a heterodimeric protein derived from the products of two genes.  The component proteins are a cytochrome P450 enzyme (encoded by the gcoA gene from the family CYP255A) and a three-domain reductase (encoded by the gcoB gene) complexed with three cofactors (2Fe-2S, FAD, and NADH).
caption: Crystal structure of gcoA (cartoon diagram) in complex with heme (green spheres) and guaiacol (magenta) based on PDB: 5NCB​.[1]
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