page_title: Total internal reflection fluorescence microscope
hierarchical_section_title: Total internal reflection fluorescence microscope
context_page_description: A total internal reflection fluorescence microscope is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed.
context_section_description: A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed.
caption: (Trans-)total internal reflection fluorescence microscope (TIRFM) diagram ObjectiveEmission beam (signal)Immersion oilCover slipSpecimenEvanescent wave rangeExcitation beamQuartz prism