page_title: Fluorescence
section_title: Biochemistry and medicine
hierarchical_section_title: Fluorescence / Applications / Biochemistry and medicine
caption_attribution_description: English: a This image is made from a Molecular Probes demo slide: Cells: bovine pulmonary arthery endothelial cells Blue: nucleus stained with DAPI Green: Tubulin (microtubles) stained with antibody Bodipy FL goat anti-mouse IgG (Indirect fluorescent antibody stain) Red: F-Actin stained with Texas Red X-Phalloidin Deutsch: Endothelzellen aus der Inneren Wand (Endothel) von Lungenarterien des Rindes unter dem Mikroskop. Die Zellkerne sind mit DAPI blau markiert. Die Mikrotubuli wurden über einen Antikörper grün markiert. Mit rot fluoreszierendem Phalloidin wurden die Aktinfilamente markiert. Français&#160;: Cellulles endothéliales vues au microscope. En bleu, noyaux marqués au DAPI. En vert, microtubules marqués par un anticorps. En rouge, actine marquée à la phalloïdine. Magyar: Fluoreszcenciamikroszkópos felvétel marha tüdőartéria endotélsejtjeiről (Molecular Probes FluoCells prepared slide #2 (F14781)). A sejtmagok DAPI-val vannak festve (kék), a mikrotubulusokhoz anti-α-tubulin egéranitest, ahhoz pedig BODIPY FL-el jelölt anti-egér kecske-IgG van kapcsolva (zöld), míg az aktin filamentumok Texas Red-X-el kapcsolt falloidinnal vannak jelölve (vörös). A kép három felvétel szuperpozíciójával készült. Hamis színek. Lietuvių: Citoskeletas. Aktino filamentai – raudona, mikrovamzdeliai – žalia, branduolys – mėlyna spalva. Română: Sub microscop Celule endoteliale . microtubulii sunt de culoare verde, iar filamentele de actină sunt roşii, pe când nucleul celulei este colorat albastru Русский: Цитоскелет эукариот. Актиновые микрофиламенты окрашены в красный (фаллоидином, связанным с TRITC), микротрубочки — в зеленый (антителами, связанными с FITC), ядра клеток — в голубой цвет (DAPI). Клетки эндотелия лёгочной артерии быка. Українська: Цитоскелет еукаріот. Актинові мікрофіламенти забарвлені в червоний колір, мікротрубочки — в зелений, ядра кліток — в блакитний
context_page_description: Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, while the emitted light is in the visible region, which gives the fluorescent substance a distinct color that can be seen only when exposed to UV light. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescent materials, which continue to emit light for some time after.
  Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors, fluorescent labelling, dyes, biological detectors, and cosmic-ray detection.
context_section_description: Fluorescence in the life sciences is used generally as a non-destructive way of tracking or analysis of biological molecules by means of the fluorescent emission at a specific frequency where there is no background from the excitation light, as relatively few cellular components are naturally fluorescent (called intrinsic or autofluorescence).
  In fact, a protein or other component can be "labelled" with an extrinsic fluorophore, a fluorescent dye that can be a small molecule, protein, or quantum dot, finding a large use in many biological applications.
  The quantification of a dye is done with a spectrofluorometer and finds additional applications in:
caption: Endothelial cells under the microscope with three separate channels marking specific cellular components
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