Материалы:NC 005957

```
LOCUS NC_005957 5237682 bp DNA circular BCT 22-JUN-2007
DEFINITION Bacillus thuringiensis serovar konkukian str. 97-27, complete
genome.
ACCESSION NC_005957
VERSION NC_005957.1 GI:49476684
KEYWORDS .
SOURCE Bacillus thuringiensis serovar konkukian str. 97-27
ORGANISM Bacillus thuringiensis serovar konkukian str. 97-27
Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus
cereus group.
REFERENCE 1 (bases 1 to 5237682)
AUTHORS Han,C.S., Xie,G., Challacombe,J.F., Altherr,M.R., Bhotika,S.S.,
Brown,N., Bruce,D., Campbell,C.S., Campbell,M.L., Chen,J.,
Chertkov,O., Cleland,C., Dimitrijevic,M., Doggett,N.A.,
Fawcett,J.J., Glavina,T., Goodwin,L.A., Green,L.D., Hill,K.K.,
Hitchcock,P., Jackson,P.J., Keim,P., Kewalramani,A.R., Longmire,J.,
Lucas,S., Malfatti,S., McMurry,K., Meincke,L.J., Misra,M.,
Moseman,B.L., Mundt,M., Munk,A.C., Okinaka,R.T.,
Parson-Quintana,B., Reilly,L.P., Richardson,P., Robinson,D.L.,
Rubin,E., Saunders,E., Tapia,R., Tesmer,J.G., Thayer,N.,
Thompson,L.S., Tice,H., Ticknor,L.O., Wills,P.L., Brettin,T.S. and
Gilna,P.
TITLE Pathogenomic sequence analysis of Bacillus cereus and Bacillus
thuringiensis isolates closely related to Bacillus anthracis
JOURNAL J. Bacteriol. 188 (9), 3382-3390 (2006)
PUBMED 16621833
REMARK Erratum:[J Bacteriol. 2006 Nov;188(21):7711. Brown, Nancy [added];
Green, Lance D [added]]
REFERENCE 2 (bases 1 to 5237682)
AUTHORS Brettin,T.S., Bruce,D., Challacombe,J.F., Gilna,P., Han,C.,
Hill,K., Hitchcock,P., Jackson,P., Keim,P., Longmire,J., Lucas,S.,
Okinaka,R., Richardson,P., Rubin,E. and Tice,H.
TITLE Complete genome sequence of Bacillus thuringiensis 97-27
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 5237682)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (11-SEP-2004) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 4 (bases 1 to 5237682)
AUTHORS Brettin,T.S., Bruce,D., Challacombe,J.F., Gilna,P., Han,C.,
Hill,K., Hitchcock,P., Jackson,P., Keim,P., Longmire,J., Lucas,S.,
Okinaka,R., Richardson,P., Rubin,E. and Tice,H.
TITLE Direct Submission
JOURNAL Submitted (07-JUN-2004) Joint Genome Institute, Department of
Energy, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from AE017355.
Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was
originally isolated from a case of severe human tissue necrosis
(Bacillus thuringiensis subsp. konkukian (serotype H34)
superinfection: Case report and experimental evidence of
pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F,
Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998
36(7):2138-2139). B. thuringiensis is indigenous to many habitats
worldwide; these include soil, insects, deciduous and coniferous
leaves (Prediction of insecticidal activity of Bacillus
thuringiensis strains by polymerase chain reaction product
profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and
Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B.
thuringiensis is an insect pathogen that is widely used as a
biopesticide in commercial agriculture. Infection of humans is
unusual. The apparent pathogenic properties of B. thuringiensis
97-27 are very unusual for B. thuringiensis; unlike most B.
thuringiensis isolates, this isolate is very closely related to B.
anthracis based on phylogenetic analysis (Fluorescent amplified
fragment length polymorphism analysis of Bacillus anthracis,
Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK,
Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M,
Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD,
Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ
Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were
prepared at the Joint Genome Institute in Los Alamos (JGI-LANL),
NM. Shotgun sequencing was performed at the JGI Production Genomics
Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x.
Finishing was performed at JGI-LANL starting with 83 contigs and 16
scaffolds. Repetitive regions were identified, assembled and
finished by manually checking paired reads close to each repeat in
the assembly with consed and then making a subassembly for each
repetitive region. Fifty five gaps were closed with primer walks
and 16 by PCR.Gene predictions were obtained using Glimmer and
tRNAs were identified using tRNAScan-SE. Basic analysis of the gene
predictions was performed by comparing coding sequences against the
PFam, BLOCKS and Prodom databases. Gene definitions and functional
classes were added manually by a team of annotators at JGI-LANL,
using BLAST results in addition to information from the basic
analysis. A total of 5540 features have been annotated on the
sequence record.
COMPLETENESS: full length.
```



## Расположение РНК

Начало | Конец | Направление | Длина | PID | Ген | COG | Комментарий |
---|---|---|---|---|---|---|---|

9337 | 10846 | + | 1510 | 49476684 | rRNA-16SrRNA_1 | - | 16S ribosomal RNA |

10994 | 11073 | + | 80 | 49476684 | tRNA-Ile-2 | - | 14 Ile tRNA
|

11079 | 11157 | + | 79 | 49476684 | tRNA-Ala-5 | - | 02 Ala tRNA
|

11249 | 14160 | + | 2912 | 49476684 | rRNA-23SrRNA_1 | - | 23S ribosomal RNA |

14209 | 14326 | + | 118 | 49476684 | rRNA-5SrRNA_1 | - | 5S ribosomal RNA |

21776 | 21871 | + | 96 | 49476684 | tRNA-Ser-5 | - | 08 Ser tRNA
|

29138 | 30647 | + | 1510 | 49476684 | rRNA-16SrRNA_2 | - | 16S ribosomal RNA |

30795 | 30874 | + | 80 | 49476684 | tRNA-Ile-4 | - | 14 Ile tRNA
|

30880 | 30958 | + | 79 | 49476684 | tRNA-Ala-1 | - | 02 Ala tRNA
|

31050 | 33960 | + | 2911 | 49476684 | rRNA-23SrRNA_2 | - | 23S ribosomal RNA |

34009 | 34126 | + | 118 | 49476684 | rRNA-5SrRNA_2 | - | 5S ribosomal RNA |

64440 | 64516 | + | 77 | 49476684 | tRNA-Met-1 | - | 16 Met tRNA
|

64527 | 64601 | + | 75 | 49476684 | tRNA-Glu-7 | - | 04 Glu tRNA
|

82466 | 83975 | + | 1510 | 49476684 | rRNA-16SrRNA_3 | - | 16S ribosomal RNA |

84151 | 87061 | + | 2911 | 49476684 | rRNA-23SrRNA_3 | - | 23S ribosomal RNA |

87162 | 87275 | + | 114 | 49476684 | rRNA-5SrRNA_3 | - | 5S ribosomal RNA |

87281 | 87359 | + | 79 | 49476684 | tRNA-Val-5 | - | 13 Val tRNA
|

87361 | 87439 | + | 79 | 49476684 | tRNA-Thr-1 | - | 12 Thr tRNA
|

87450 | 87528 | + | 79 | 49476684 | tRNA-Lys-4 | - | 10 Lys tRNA
|

87541 | 87624 | + | 84 | 49476684 | tRNA-Leu-8 | - | 15 Leu tRNA
|

87651 | 87728 | + | 78 | 49476684 | tRNA-Gly-2 | - | 01 Gly tRNA
|

87742 | 87833 | + | 92 | 49476684 | tRNA-Leu-6 | - | 15 Leu tRNA
|

87834 | 87910 | + | 77 | 49476684 | tRNA-Arg-5 | - | 11 Arg tRNA
|

87916 | 87992 | + | 77 | 49476684 | tRNA-Pro-2 | - | 03 Pro tRNA
|

87999 | 88077 | + | 79 | 49476684 | tRNA-Ala-3 | - | 02 Ala tRNA
|

88179 | 89688 | + | 1510 | 49476684 | rRNA-16SrRNA_4 | - | 16S ribosomal RNA |

89864 | 92774 | + | 2911 | 49476684 | rRNA-23SrRNA_4 | - | 23S ribosomal RNA |

92822 | 92939 | + | 118 | 49476684 | rRNA-5SrRNA_4 | - | 5S ribosomal RNA |

151247 | 152755 | + | 1509 | 49476684 | rRNA-16SrRNA_5 | - | 16S ribosomal RNA |

152931 | 155841 | + | 2911 | 49476684 | rRNA-23SrRNA_5 | - | 23S ribosomal RNA |

155927 | 156040 | + | 114 | 49476684 | rRNA-5SrRNA_5 | - | 5S ribosomal RNA |

156050 | 156127 | + | 78 | 49476684 | tRNA-Asn-3 | - | 07 Asn tRNA
|

156129 | 156204 | + | 76 | 49476684 | tRNA-Thr-3 | - | 12 Thr tRNA
|

156226 | 156303 | + | 78 | 49476684 | tRNA-Glu-4 | - | 04 Glu tRNA
|

156306 | 156384 | + | 79 | 49476684 | tRNA-Val-6 | - | 13 Val tRNA
|

156399 | 156485 | + | 87 | 49476684 | tRNA-Tyr-2 | - | 18 Tyr tRNA
|

156548 | 156625 | + | 78 | 49476684 | tRNA-Gln-4 | - | 05 Gln tRNA
|

156628 | 156706 | + | 79 | 49476684 | tRNA-Lys-1 | - | 10 Lys tRNA
|

156709 | 156783 | + | 75 | 49476684 | tRNA-Gly-6 | - | 01 Gly tRNA
|

156791 | 156866 | + | 76 | 49476684 | tRNA-Ala-2 | - | 02 Ala tRNA
|

251006 | 251083 | + | 78 | 49476684 | tRNA-Asn-1 | - | 07 Asn tRNA
|

251084 | 251177 | + | 94 | 49476684 | tRNA-Ser-2 | - | 08 Ser tRNA
|

251183 | 251260 | + | 78 | 49476684 | tRNA-Glu-1 | - | 04 Glu tRNA
|

251262 | 251340 | + | 79 | 49476684 | tRNA-Val-3 | - | 13 Val tRNA
|

251384 | 251462 | + | 79 | 49476684 | tRNA-Asp-4 | - | 06 Asp tRNA
|

251547 | 251624 | + | 78 | 49476684 | tRNA-Gln-3 | - | 05 Gln tRNA
|

251627 | 251702 | + | 76 | 49476684 | tRNA-Lys-5 | - | 10 Lys tRNA
|

251716 | 251804 | + | 89 | 49476684 | tRNA-Leu-5 | - | 15 Leu tRNA
|

251897 | 251976 | + | 80 | 49476684 | tRNA-Arg-3 | - | 11 Arg tRNA
|

251978 | 252057 | + | 80 | 49476684 | tRNA-Pro-4 | - | 03 Pro tRNA
|

252056 | 252129 | + | 74 | 49476684 | tRNA-Gly-3 | - | 01 Gly tRNA
|

252230 | 253739 | + | 1510 | 49476684 | rRNA-16SrRNA_6 | - | 16S ribosomal RNA |

253915 | 256825 | + | 2911 | 49476684 | rRNA-23SrRNA_6 | - | 23S ribosomal RNA |

256874 | 256991 | + | 118 | 49476684 | rRNA-5SrRNA_6 | - | 5S ribosomal RNA |

257001 | 257080 | + | 80 | 49476684 | tRNA-Met-4 | - | 16 Met tRNA
|

257081 | 257159 | + | 79 | 49476684 | tRNA-Asp-1 | - | 06 Asp tRNA
|

272749 | 274258 | + | 1510 | 49476684 | rRNA-16SrRNA_7 | - | 16S ribosomal RNA |

274434 | 277344 | + | 2911 | 49476684 | rRNA-23SrRNA_7 | - | 23S ribosomal RNA |

277392 | 277509 | + | 118 | 49476684 | rRNA-5SrRNA_7 | - | 5S ribosomal RNA |

279760 | 281269 | + | 1510 | 49476684 | rRNA-16SrRNA_8 | - | 16S ribosomal RNA |

281445 | 284355 | + | 2911 | 49476684 | rRNA-23SrRNA_8 | - | 23S ribosomal RNA |

284402 | 284519 | + | 118 | 49476684 | rRNA-5SrRNA_8 | - | 5S ribosomal RNA |

287106 | 288614 | + | 1509 | 49476684 | rRNA-16SrRNA_9 | - | 16S ribosomal RNA |

291748 | 291865 | + | 118 | 49476684 | rRNA-5SrRNA_9 | - | 5S ribosomal RNA |

297075 | 298584 | + | 1510 | 49476684 | rRNA-16SrRNA_10 | - | 16S ribosomal RNA |

298760 | 301671 | + | 2912 | 49476684 | rRNA-23SrRNA_9 | - | 23S ribosomal RNA |

301718 | 301836 | + | 119 | 49476684 | BT9727_r02 | - | 5S ribosomal RNA |

304222 | 305776 | + | 1555 | 49476684 | BT9727_r01 | - | 16S ribosomal RNA |

305935 | 308846 | + | 2912 | 49476684 | rRNA-23SrRNA_10 | - | 23S ribosomal RNA |

308895 | 309012 | + | 118 | 49476684 | rRNA-5SrRNA_10 | - | 5S ribosomal RNA |

521476 | 522985 | + | 1510 | 49476684 | rRNA-16SrRNA_11 | - | 16S ribosomal RNA |

523160 | 526070 | + | 2911 | 49476684 | rRNA-23SrRNA_11 | - | 23S ribosomal RNA |

526171 | 526284 | + | 114 | 49476684 | rRNA-5SrRNA_11 | - | 5S ribosomal RNA |

526293 | 526370 | + | 78 | 49476684 | tRNA-Asn-4 | - | 07 Asn tRNA
|

526370 | 526464 | + | 95 | 49476684 | tRNA-Ser-3 | - | 08 Ser tRNA
|

526479 | 526556 | + | 78 | 49476684 | tRNA-Glu-3 | - | 04 Glu tRNA
|

526559 | 526637 | + | 79 | 49476684 | tRNA-Val-4 | - | 13 Val tRNA
|

526659 | 526738 | + | 80 | 49476684 | tRNA-Met-3 | - | 16 Met tRNA
|

526739 | 526817 | + | 79 | 49476684 | tRNA-Asp-5 | - | 06 Asp tRNA
|

526824 | 526902 | + | 79 | 49476684 | tRNA-Phe-2 | - | 17 Phe tRNA
|

526918 | 526996 | + | 79 | 49476684 | tRNA-Thr-5 | - | 12 Thr tRNA
|

527004 | 527090 | + | 87 | 49476684 | tRNA-Tyr-1 | - | 18 Tyr tRNA
|

527095 | 527171 | + | 77 | 49476684 | tRNA-Trp-2 | - | 20 Trp tRNA
|

527188 | 527266 | + | 79 | 49476684 | tRNA-His-1 | - | 09 His tRNA
|

527327 | 527404 | + | 78 | 49476684 | tRNA-Gln-2 | - | 05 Gln tRNA
|

527407 | 527484 | + | 78 | 49476684 | tRNA-Gly-9 | - | 01 Gly tRNA
|

527496 | 527569 | + | 74 | 49476684 | tRNA-Cys-1 | - | 19 Cys tRNA
|

527577 | 527664 | + | 88 | 49476684 | tRNA-Leu-2 | - | 15 Leu tRNA
|

532092 | 532168 | + | 77 | 49476684 | tRNA-Gly-8 | - | 01 Gly tRNA
|

732426 | 733935 | + | 1510 | 49476684 | rRNA-16SrRNA_12 | - | 16S ribosomal RNA |

734110 | 737020 | + | 2911 | 49476684 | rRNA-23SrRNA_12 | - | 23S ribosomal RNA |

737121 | 737234 | + | 114 | 49476684 | rRNA-5SrRNA_12 | - | 5S ribosomal RNA |

737240 | 737318 | + | 79 | 49476684 | tRNA-Val-7 | - | 13 Val tRNA
|

737324 | 737410 | + | 87 | 49476684 | tRNA-Tyr-3 | - | 18 Tyr tRNA
|

737419 | 737496 | + | 78 | 49476684 | tRNA-Gln-1 | - | 05 Gln tRNA
|

737498 | 737576 | + | 79 | 49476684 | tRNA-Lys-6 | - | 10 Lys tRNA
|

737588 | 737671 | + | 84 | 49476684 | tRNA-Leu-1 | - | 15 Leu tRNA
|

737698 | 737775 | + | 78 | 49476684 | tRNA-Gly-4 | - | 01 Gly tRNA
|

737789 | 737880 | + | 92 | 49476684 | tRNA-Leu-7 | - | 15 Leu tRNA
|

737881 | 737957 | + | 77 | 49476684 | tRNA-Arg-1 | - | 11 Arg tRNA
|

737965 | 738041 | + | 77 | 49476684 | tRNA-Pro-3 | - | 03 Pro tRNA
|

738055 | 738130 | + | 76 | 49476684 | tRNA-Ala-6 | - | 02 Ala tRNA
|

738148 | 738243 | + | 96 | 49476684 | tRNA-Ser-6 | - | 08 Ser tRNA
|

738296 | 738391 | + | 96 | 49476684 | tRNA-Ser-1 | - | 08 Ser tRNA
|

738409 | 738488 | + | 80 | 49476684 | tRNA-Met-6 | - | 16 Met tRNA
|

738490 | 738568 | + | 79 | 49476684 | tRNA-Asp-3 | - | 06 Asp tRNA
|

738574 | 738652 | + | 79 | 49476684 | tRNA-Phe-1 | - | 17 Phe tRNA
|

738659 | 738734 | + | 76 | 49476684 | tRNA-Thr-6 | - | 12 Thr tRNA
|

738738 | 738811 | + | 74 | 49476684 | tRNA-Trp-1 | - | 20 Trp tRNA
|

738822 | 738901 | + | 80 | 49476684 | tRNA-Ile-3 | - | 14 Ile tRNA
|

738907 | 738984 | + | 78 | 49476684 | tRNA-Asn-5 | - | 07 Asn tRNA
|

738983 | 739057 | + | 75 | 49476684 | tRNA-Glu-5 | - | 04 Glu tRNA
|

969811 | 969884 | + | 74 | 49476684 | tRNA-Cys-2 | - | 19 Cys tRNA
|

1255195 | 1255270 | - | 76 | 49476684 | tRNA-Val-2 | - | 13 Val tRNA
|

4082558 | 4082634 | - | 77 | 49476684 | tRNA-Met-7 | - | 16 Met tRNA
|

4280195 | 4280271 | - | 77 | 49476684 | tRNA-Gly-7 | - | 01 Gly tRNA
|

4655315 | 4655389 | - | 75 | 49476684 | tRNA-Glu-2 | - | 04 Glu tRNA
|

4655393 | 4655486 | - | 94 | 49476684 | tRNA-Ser-7 | - | 08 Ser tRNA
|

4655491 | 4655568 | - | 78 | 49476684 | tRNA-Asn-2 | - | 07 Asn tRNA
|

4655573 | 4655652 | - | 80 | 49476684 | tRNA-Ile-1 | - | 14 Ile tRNA
|

4655660 | 4655733 | - | 74 | 49476684 | tRNA-Gly-1 | - | 01 Gly tRNA
|

4655744 | 4655822 | - | 79 | 49476684 | tRNA-Lys-3 | - | 10 Lys tRNA
|

4655830 | 4655908 | - | 79 | 49476684 | tRNA-Thr-2 | - | 12 Thr tRNA
|

4655920 | 4655998 | - | 79 | 49476684 | tRNA-Phe-3 | - | 17 Phe tRNA
|

4656008 | 4656086 | - | 79 | 49476684 | tRNA-Asp-2 | - | 06 Asp tRNA
|

4656085 | 4656164 | - | 80 | 49476684 | tRNA-Met-5 | - | 16 Met tRNA
|

4656189 | 4656284 | - | 96 | 49476684 | tRNA-Ser-4 | - | 08 Ser tRNA
|

4656299 | 4656378 | - | 80 | 49476684 | tRNA-Met-8 | - | 16 Met tRNA
|

4656380 | 4656459 | - | 80 | 49476684 | tRNA-Met-2 | - | 16 Met tRNA
|

4656477 | 4656552 | - | 76 | 49476684 | tRNA-Ala-4 | - | 02 Ala tRNA
|

4656565 | 4656641 | - | 77 | 49476684 | tRNA-Pro-1 | - | 03 Pro tRNA
|

4656649 | 4656725 | - | 77 | 49476684 | tRNA-Arg-4 | - | 11 Arg tRNA
|

4656726 | 4656817 | - | 92 | 49476684 | tRNA-Leu-4 | - | 15 Leu tRNA
|

4656831 | 4656908 | - | 78 | 49476684 | tRNA-Gly-5 | - | 01 Gly tRNA
|

4656935 | 4657018 | - | 84 | 49476684 | tRNA-Leu-3 | - | 15 Leu tRNA
|

4657038 | 4657116 | - | 79 | 49476684 | tRNA-His-2 | - | 09 His tRNA
|

4657123 | 4657201 | - | 79 | 49476684 | tRNA-Thr-4 | - | 12 Thr tRNA
|

4657203 | 4657281 | - | 79 | 49476684 | tRNA-Val-1 | - | 13 Val tRNA
|

4657286 | 4657399 | - | 114 | 49476684 | rRNA-5SrRNA_13 | - | 5S ribosomal RNA |

4657500 | 4660410 | - | 2911 | 49476684 | rRNA-23SrRNA_13 | - | 23S ribosomal RNA |

4660586 | 4662095 | - | 1510 | 49476684 | rRNA-16SrRNA_13 | - | 16S ribosomal RNA |

4873207 | 4873285 | - | 79 | 49476684 | tRNA-Arg-2 | - | 11 Arg tRNA
|

5217059 | 5217134 | - | 76 | 49476684 | tRNA-Phe-4 | - | 17 Phe tRNA
|

5217173 | 5217251 | - | 79 | 49476684 | tRNA-Asp-6 | - | 06 Asp tRNA
|

5217271 | 5217345 | - | 75 | 49476684 | tRNA-Glu-6 | - | 04 Glu tRNA
|

5217356 | 5217434 | - | 79 | 49476684 | tRNA-Lys-2 | - | 10 Lys tRNA
|