Cognitive Dysfunction Following Cardiac Surgery

Postoperative cognitive dysfunction (POCD) is the most common complication after cardiac surgery. This prospective study was conducted to investigate the mechanisms of development of POCD following cardiac surgery taking into account surgical technique (with use or no use of extracorporeal circulation). The investigators focused on the role of inflammatory and stress response to surgical procedure as potential factors involved in the pathogenesis of cognitive dysfunction. Systematic inflammatory response in patients undergoing on-pump or off-pump surgery was analyzed by measuring serum levels of C reactive protein (CRP) and occurrence of systemic inflammatory response syndrome (SIRS). Stress response to surgery was evaluated following cortisol levels and its daily variations. The degree of cognitive dysfunction was assessed based on serum levels of S100β. The effect of dexamethasone on the levels of stress and inflammatory response biomarkers, serum levels of S100β, as well as on the development of POCD was compared with control group that received normal saline.

This is a prospective, randomized, double blind, controlled trial. Following detailed past medical history assessment, two days before the surgical procedure neurocognitive function tests, depression and anxiety tests were carried out to assess the participants. Neurocognitive and anxiety tests were repeated on the 6th day after surgical procedure. A battery of tests lasting approximately 40 minutes were administered by a trained neuropsychologist from the Clinical Hospital Center, Split in a standardized fashion, at the same time of the day (10:00 AM) and in the same room at the Cardiac surgery ward. The investigators used a validated battery of tests which enabled the assessment of psychomotor speed, visual-motor coordination, concentration, attention, short-term memory and aspects of executive functioning. The evaluation was based on the following tests: Mini Mental State Examination (MMSE), Wechsler Memory Scale (WMS I \& II), Trail Making Test (TMT A \& B), Symbol Digit Modalities Test (SDMT), Controlled Oral Word Association Test (COWAT) and PsychE computer test. All of the tests except TMT A \& B have alternate forms. Croatian versions of Beck Depression Inventory-Second Edition (BDI II) and State-Trait Anxiety Inventory (STAI-M) were used for preoperative depression and trait and state anxiety assessment, respectively.

One day before the surgery (08:00 AM) blood samples were collected for determination of levels of S100β protein, cortisol and other routine laboratory parameters (white blood cell count (WBC), hematocrit, creatinine, glucose, albumin, sodium, potassium, C reactive protein (CRP), Troponin I hs).

Participants were randomized into two groups to receive either intravenous dexamethasone 0.1 mg/kg or the same volume of normal saline i.v. 10 hours before the surgery. Random sequence generator was used to determine participant allocation.

Anesthesia in all patients was based on fentanyl, midazolam, vecuronium and sevoflurane. The depth of anesthesia was monitored using bispectral index (BIS). During cardiopulmonary bypass alpha-stat technique was used with the maintenance of normothermia (35.5-36.5°C) or spontaneous hypothermia (up to 32°C). With the off-pump technique, the core temperature was maintained between 36 and 37°C. Surgical technique was standardized. Routine hemodynamic monitoring was performed throughout the surgery and continued on the first postoperative day. Plasma levels of S100β protein were determined 6 h and 30 h following the end of on-pump surgery, and 3 h following the end of off-pump surgery. C reactive protein levels and other routine laboratory parameters were measured from blood samples collected 1 h, 4 h and 12 h postoperatively. On the first postoperative day blood samples were collected at 08:00 AM to determine cortisol levels, C reactive protein and other routine laboratory parameters, and cortisol was also measured on the same day at 04:00 PM, and 12:00 AM. C reactive protein and other routine laboratory parameters were repeatedly measured at 08:00 AM on the following postoperative days, except for cortisol levels which were measured on postoperative days 3 and 5. Registered nurse carried out the standardized test (Delirium Observation Screening Scale (DOS) scale) to assess the patients for the development of postoperative delirium from second to fifth postoperative day (at 07:00 AM, 02:00 PM and 10:00 PM).

Eligibility Criteria:
Inclusion Criteria:

* Participants undergoing cardiac surgery: coronary artery bypass grafting, heart valve surgery or a combined procedure,
* Procedure must be elective

Exclusion Criteria:

* Symptomatic cerebrovascular disease,
* Cerebrovascular incident in last 3 years,
* Neurodegenerative disease,
* Psychiatric disease,
* Visual, hearing or motor impairment interfering with cognitive testing,
* Carotid artery stenosis ( ≥50%),
* Myocardial infarction within last 3 months,
* Left ventricular ejection fraction of ≤35%,
* Previous cardiac or carotid surgery,
* Uncontrolled systemic hypertension (blood pressure≥180/110 mmHg),
* Corticosteroid-dependent asthma,
* Chronic obstructive pulmonary disease (COPD),
* Malignancy, liver disease (aspartate transaminase (AST), alanine transaminase (ALT) or bilirubin ˃1.5x above reference range),
* Kidney disease (serum creatinine ˃1.7 mg/dl or blood urea nitrogen ˃50 mg/dL),
* Uncontrolled diabetes mellitus (postprandial glucose ˃200 mg/dL or hemoglobin A1c ˃9%),
* Endocrine and metabolic diseases, requiring steroid treatment longer than 7 days in the past year,
* Alcohol or controlled substance abuse,
* Individuals without primary school education,
* Preoperative C reactive protein ˃0.5 mg/dL,
* Preoperative white blood cell count \<3 x109/L or ˃11 x109/L,
* Preoperative Mini mental score \<26 points,
* Preoperative Beck's depression inventory score ˃ 19 points,
* Intraoperative plan change (conversion from "off-pump" to "on-pump" and vice versa),
* Need for deep hypothermic circulatory arrest,
* Additional corticosteroid treatment throughout the study period.