cyrilzakka/clinical-trials huggingface dataset:
HCV Virions Bound Proteins

The emergence of hepatocellular carcinoma (HCC) has prompted a search for a thorough understanding of the biology of one of its major causative agents, the hepatitis C virus (HCV). HCV particles acquire via budding and encapsidation cellular proteins. There is mounting evidence on several viral species that virion-bound proteins are prone to be involved either at the replication, budding/egress or entry/release steps of the viral cycle.

Identifying such targets may yield ideal candidates for gaining insight on the dependence of HCV upon a restricted subset of host proteins, therefore providing refined sets of genetically stable targets for therapy. This project's goals are to set up adequate conditions for robust and reproducible purification of HCV virions in clinical samples, followed by the identification of their HCV-bound host proteins and the characterization of their functions. Proteomics profiling of HCV particles purified from clinical samples will be overlaid with proteins identified and characterized in cell culture grown HCV particles during my post-doctoral training, using clinical biomarker discovery grade criteria. Targets identified in both samples sets will be subjected to in vitro investigations using HCV-replicating cells. Conventional biochemical and imaging methods will be used in order to: (i) ascertain their physical association with HCV virions; (ii) define the modalities of their interaction with HCV proteins; (iii) decipher the topology and subcellular localization of their association with HCV proteins and virions; (iv) quantitatively assess their functional involvement in particle budding, egress or secretion and infectivity. A candidate that yielded satisfactory results in these experiments will be disclosed and further investigated at the level of structural biology, in collaborative research programs.


Eligibility Criteria:
Inclusion Criteria:

* Adult\> 18 and \<60 years
* Infected with HCV genotype 1 HCV antibody positive.
* positive viremia for more than 6 months
* Viremia\> 106 IU / ml.
* nonresponders to previous treatment and without antiviral treatment for 2 months.
* For control samples: Patients responders to previous treatment and without antiviral treatment for 2 months.

Exclusion Criteria:

* Patient receiving or having received antiviral treatment within two months.
* patient with against-indication for a blood sample of 150 ml
* immunosuppressive therapy patient
* Patient with liver disease other than hepatitis C.
* Patients with cirrhosis.
* patient with hepatocellular carcinoma.
* Patients with one or more severe co-morbidities defined as:
* Co-infection with HIV or HBV.
* hematological malignancies changing or aplasia
* Insulin-dependent diabetes
* dialyzed chronic renal failure
* Heart failure
* Persons subject to legal protection or the subject of a safeguard measure of justice not affiliated with a social security scheme or not beneficiaries of such a scheme
* Pregnant women
Effectiveness of Internet-based Injury Prevention Program in Enhancing Mother's Knowledge on Child Safety

Background: Provision of anticipatory guidance to parents is recommended as an effective strategy to prevent injuries among young children. Internet-based anticipatory guidance is suggested to reinforce the effectiveness of injury prevention, and improve parents' knowledge on child safety. Parents receiving the guidance can reduce children's exposure to injury risk by adopting better childcare practices and using appropriate child safety devices at home.

Objective: This study will examine the effectiveness of Internet-based injury prevention program with parental anticipatory guidance in enhancing mothers' knowledge on child safety. It aims at increasing mothers' knowledge and motivation of learning about domestic injury prevention through a new Internet-based intervention model. It also targets to improve mothers' attitude and perceived behavioral control of domestic safety practice.

Methods: The study would adopt a randomized controlled trial design and recruit 934 mothers from the antenatal clinics and postnatal wards of two major public hospitals in Hong Kong. Participating mothers will be randomized into the intervention or control group with equal likelihood. Mothers in intervention group will be provided with free access to an Internet-based injury prevention program with anticipatory guideline whereas those in the control group will receive relevant parenting booklet.

Results: It is hypothesized that mothers' general and age-appropriate knowledge on child safety and motivation of learning about domestic injury prevention as the primary outcome measures will be enhanced.

Conclusions: The Internet is increasingly recognized as a practical and cost-effective platform for health education and safety information delivery. The goals of this study are to examine the effectiveness of a new Internet-based intervention program for improving mothers' knowledge, and raising mothers' awareness about the importance and consequences caused by domestic injuries.


Eligibility Criteria:
Inclusion Criteria:

* Mothers attending the antenatal clinics/staying in the postnatal wards at Kwong Wah Hospital or Queen Mary Hospital.

Exclusion Criteria:

* Mothers unable to read Chinese and those without access to Internet.
Safety of the Etonogestrel-releasing Implant During the Puerperium of Healthy Women

The purpose of this study to assess the safety of the etonogestrel-releasing subdermal implant (Implanon) inserted during the immediate puerperium of healthy women.

Many contraceptive methods are currently available. However, about 50% of all pregnancies in the world are not planned, most of them occurring in developing countries. Long-lasting reversible contraceptives such as the etonogestrel implant represent an option for the reduction of unwanted pregnancies, especially among patients at risk for a short intergestational period. In addition to preventing an undesired pregnancy, these methods have an impact on the reduction of the maternal-fetal morbidity-mortality known to be associated with these short intervals, also minimizing the malnutrition and the cycle of poverty caused by multiparity.

On the basis of inclusion and exclusion criteria, we will selected 40 puerperae aged 18 to 35 years at the Low Risk Prenatal Care Program of the University Hospital of Ribeirão Preto, University of São Paulo (HC-FMRP). The subjects will be randomized to two types of treatment (etonogestrel-releasing implant to be inserted 24 to 48 hours after delivery or 150 mg medroxyprogesterone administered every three months starting 6 weeks after delivery). Blood samples (40 mL) will be collected in a single procedure from these patients and stored for later determination of multiple hemostatic and metabolic variables at 24-48 hours and at 6 and 12 weeks after delivery. Data on maternal and neonatal clinical parameter will be also collected.

Eligibility Criteria:
Inclusion Criteria:

* age between 18 and 35 years
* Postpartum contraception desire

Exclusion Criteria:

* smoking, alcoholism or drug addiction
* presence of systemic diseases (diabetis melittus, cardiovascular disease, autoimmune diseases, liver disease, thyroid disease, or congenital renal hyperplasia)
* having a body mass index ≥ 30 kg/m2
* personal history of arterial or venous thrombosis
* using any medication that might interfere with blood coagulation or with the assessment of haemostatic and inflammatory variables
* presenting alterations in hepatic enzymes
* being allergic to local anaesthetics (xylocaine)
Effects of tDCS on Motor Cortex During ACL Recovery

Anterior Cruciate Ligament (ACL) is a prevalent injury, particularly among young and physically active individuals. The efficacy of transcranial direct current stimulation (tDCS) and exercise-based rehabilitation on neuromuscular control in post-ACL reconstruction patients is evaluated, and these techniques are combined.

Anterior cruciate ligament (ACL) rupture is a common injury, particularly among young and physically active individuals, with an incidence of 0.4 to 0.8 injuries per 1,000 person-years. While the majority of ruptures occur during sports activities (65-75%), a significant proportion (25-35%) happen in non-sport settings. Rehabilitation is crucial for recovery, but only 65% of patients return to their pre-injury activity level, and only 55% resume competitive activities. Neuromuscular structures such as the hamstrings and hip abductors play a vital role in reducing the risk of re-injury and aiding post-surgical rehabilitation.

Arthrogenic muscle inhibition (AMI) is a common phenomenon following ACL surgery, affecting quadriceps activation and force generation. While spinal mechanisms of AMI have been extensively studied, the influence of supraspinal centers, such as the motor cortex, in modulating AMI is also recognized. Traditional treatments, such as electrostimulation, are largely ineffective, and non-invasive brain stimulation techniques, such as transcranial direct current stimulation (tDCS), combined with exercise rehabilitation, are being explored to improve neuromuscular control.

This study aims to evaluate the effects of combined tDCS and exercise-based rehabilitation, comparing it to sham tDCS treatment. Outcomes will include cortical reorganization, corticospinal activation, pain perception, and psychosocial and functional variables. The central hypothesis is that reducing cortical hyperexcitability will enhance neuromuscular control, leading to improved outcomes and a reduced risk of re-injury.

Eligibility Criteria:
Inclusion Criteria:

* Diagnosis complete ACL tear through clinical evaluation and MRI imaging.
* Patients who have received surgical intervention.
* Aged between 16 and 40 years.
* Tegner activity level of 4 or higher.

Exclusion Criteria:

* Rupture, such as tendons, cartilage, bones, or ligaments.
* Absence of any pre-existing or current lower limb pathologies, such as open surgeries, knee arthroscopies, or femur/tibia fractures.
* Neuromuscular or metabolic diseases.
* Concussion within the past six months are not eligible.
* Cranial surgery or have intracranial metal clips are ineligible.
* Taking medications that affect neuronal activity.
* Neurological diseases or disorders are not eligible for participation.
Effect of Aging on Bariatric Surgery-induced Changes in Metabolism and Cognition

The investigators hypothesize that the improvements in insulin sensitivity, beta (β)-cell function, and inflammation will be greater, and the improvement in sarcopenic obesity will be less, in younger versus older individuals after substantial weight loss induced by sleeve gastrectomy bariatric surgery.

With an aging population that is increasingly susceptible to obesity and obesity-related comorbidities including sarcopenia and diabetes, effective and safe treatment options tailored to the needs of older adults are imperative. While medication and lifestyle interventions generally fail to achieve sustained large-scale weight loss, bariatric surgery is the most effective long-term weight loss treatment for obese patients and improves many of the medical complications associated with obesity. Sleeve gastrectomy (SG) results in substantial weight loss and may be more appropriate for older adults due to its low rate of complications and mortality. Therefore, SG has been proposed as a reasonable primary treatment modality in older obese individuals, with a substantial recent increase in the number of interventions performed. Despite the potential benefits, very little is known of the physiologic and metabolic effects of bariatric surgery, including SG, on glucose homeostasis and muscle physiology in older adults. A fundamental understanding of the effects of excess adiposity and weight loss interventions is of profound importance, especially with emerging evidence that earlier treatment of obesity may delay/prevent many comorbidities. An underlying inflammatory state has been implicated in the pathogenesis of sarcopenia and insulin resistance and may provide an important connection to the age-related declines seen in older obese patients. Therefore, this study aims to evaluate the modifying effect of age and inflammation on the SG-induced changes in body composition, muscle physiology, and insulin sensitivity. This protocol will be conducted in two age cohorts (greater than 60 and less than 50 years old).

Eligibility Criteria:
Inclusion Criteria:

* Age 21-80 yrs old
* BMI ≥ 35 kg/m2
* Undergoing laparoscopic sleeve gastrectomy (SG)

Exclusion Criteria:

* Smokers
* Presence of diabetes mellitus
* Taking any medication that might affect metabolism
* Severe organ dysfunction
* Pregnant or breastfeeding
* Prior gastrointestinal surgery that might affect study results
* Unable or unwilling to follow the study protocol or any reason the research team believes the subject is not an appropriate candidate for this study
* Weight \>450 pounds (Will be unable to fit on the DXA scanner)
Effect of Propofol on Internal Anal Sphincter Pressure During ARM

Background and Aim: An anorectal manometry (ARM) is a common test in the evaluation of outlet obstruction in constipation. In children under 6 yo, anesthesia is often used for patient comfort and compliance. The choice of anesthesia for ARM varies from center to center and includes the use of ketamine, inhalation agents, and propofol. With the increased use of propofol, our aim is to determine the effects of propofol on resting anal sphincter pressure while performing an ARM under general anesthesia.

Hypothesis: Propofol administration leads to a decrease in internal anal sphincter pressure.

Methods: This is a prospective, single center, observational study of the effect of propofol on the internal anal sphincter pressure. Pediatric patients between the ages of 2-6 years who require an anorectal manometry for chronic constipation will be offered enrollment. Anesthesia was required for patient compliance and comfort and conducted by an anesthesiologist. Sevoflurane was the inhalational agent used for induction. At Massachusetts General Hospital (MGH), 1 mg/kg of propofol bolus is routinely given at completion of the ARM test to reduce emergence delirium. The manometric probe was kept in place with continuous measurement of baseline internal anal sphincter pressure while propofol was administered. We measured changes to internal anal sphincter pressure and time to return to baseline.

Results: The primary outcomes will be change of internal anal sphincter pressure from baseline and time to return to baseline.

Discussion: The proposed investigation may demonstrate that propofol has an effect on the internal anal sphincter pressure which may affect its utility as an anesthetic for anorectal manometry.


Eligibility Criteria:
Inclusion Criteria:

* Ages 2 - 6 years
* Chronic constipation
* Requires anorectal manometry

Exclusion Criteria:

* Ages 0-2 or \> 6 years
* Abnormal anorectal anatomy
* Known history of Hirschsprung's Disease
Use of Dynamic Elastometric Body in Preterm Newborns

The goal of this prospective pilot study is to test the use of dynamic elastomeric body in a population of preterm newborns with hyperexcitability syndrome.

The main questions it aims to answer are:

* To evaluate the effectiveness of the body in elasto-compressive material (FLEXA) in addition to standard care;
* To evaluate how the use of dynamic elastomeric body promote postural containment, reduce hyperexcitability (tremor and crying), improve the organization of movement and reduce respiratory distress.

Participants will be given to routine clinical evaluations that are part of the standard of care of the premature infant admitted to Neonatology.

The clinical evaluation shall consist of:

* Hammersmith neonatal neurological examination;
* Neonatal Intensive Care Unit Network Neurobehavioural Scale (NNNS);
* General Movement's (GM's)
* Goal Attainment Scaling (GAS) Researchers will compare a study group that will be subjected to treatment with the body in elasto-compressive material and a control group without using the body in elasto-compressive material.


Eligibility Criteria:
Inclusion Criteria:

* Signs of hyperexcitability such as tremors, sudden and chaotic movements, prevalent extensor tone, postural instability and/or neurovegetative phenomena;
* Low neurological risk, i.e., according to the protocols implemented in the standard clinical practice of the Neonatology unit, a cerebral ultrasound is performed for those born between 28-32 weeks of GA, and for those born before 28 weeks of GA, an MRI of the brain) from which it may derive;
* Cerebral ultrasound normal or with minor ultrasound findings such as grade 1 intraventricular hemorrhage (germinal matrix hemorrhage with intraventricular hemorrhage covering less than 10% of the ventricular area in the parasagittal window), or evidence of transient focal white matter hyperechogenicity.
* Issue of informed consent by the parent or legal guardian.

Exclusion Criteria:

* Neuroimaging findings of major brain lesions, such as evidence of grade 2 or greater intraventricular hemorrhage on cerebral ultrasonography (germinal matrix hemorrhage with intraventricular hemorrhage over 10% of the ventricular area in the parasagittal window/with periventricular hyperechogenicity ) or persistent hyperechogenicity for more than a week;
* Presence of: ongoing infectious states and/or sepsis, ostomy wearers, severe cardio-respiratory disorders at the time of enrolment; diagnosis of genetic or metabolic diseases; carriers of cerebral malformations; invasive or non-invasive respiratory assistance;
* Failure to issue informed consent by the parent or legal guardian.
Intrathecal Ketorolac in Patients With Intrathecal Morphine Dose Escalation

The purpose of this study is to determine how the drug ketorolac, when given with the spinal morphine, affects pain.

This study is part of a pain center grant that focuses on how pain, especially chronic neuropathic pain, alters the response to traditional and non-traditional analgesics (pain medications).

To treat persons with chronic pain, doctors sometimes implant a permanent tube in the spinal space in the back to give pain medicines. This tube, also called a spinal pump, is used to administer pain medicine directly to the spinal space. The most commonly used medicine given through the spinal pump is morphine which works well for most people, but sometimes loses tolerance, and larger and larger doses are needed, causing many side effects.

The purpose of this study is to find out if another medicine, ketorolac, when given with morphine in the spinal space, can stop or reverse the need for larger and larger doses of morphine. In animals, ketorolac has been shown to slow the need for an increase in morphine dosage and to reduce the morphine dose, while giving good pain control.

This study will enroll 30 individuals with chronic pain below the chest, caused by nerve injury, who are receiving morphine through a spinal pump. After learning to estimate pain accurately using thermal heat testing, participants will be randomly assigned to one of two study groups. Group one will receive the active study medication, ketorolac, while group two receives an inactive control (placebo). All participants will receive morphine (administered through the spinal pump). Afterwards, participants will rate their levels of pain, and provide samples of their spinal fluid (taken from the spinal tube/pump) for study. The above procedure will be repeated over a 4-week period.

Duration of the study for participants is 4 weeks, and includes ten visits to the research center, each lasting less than one hour.

Eligibility Criteria:
Inclusion Criteria:

* Implanted spinal pump for at last 9 months
* Must be receiving and the dose must have been doubled in the past 6 months
* Currently taking 60-200 mg morphine equivalent as oral rescue per day
* Men and women, ages 18-70
* Weigh no more that 250 pounds
* Neuropathic pain

Exclusion Criteria:

* Pregnancy
* Unstable medical problems (heart lung, liver, kidney, or nervous system)
* Allergy to morphine, ketorolac, or drugs which may be used to treat side effects
Oral Cavity Squamous Cell Carcinoma and Readmission: Rates, Causes, and Risk Factors.

We sought to comprehend the rates and causes of unplanned hospital readmission within 60 days following oral cancer surgery


Eligibility Criteria:
Inclusion Criteria:

* all patient underwent oral cancer surgery in king Abdulaziz University hospital

Exclusion Criteria:

* significant missing data
[68Ga]Ga-NOTA-RW102 PET Imaging in the Diagnosis of PD-L1-expressing Lung Cancer

The aim of this study was to establish and optimize the imaging method of \[68Ga\]Ga-NOTA-RW102, as well as its physiological and pathological distribution characteristics, on the basis of which the diagnostic efficacy of the above imaging agent was evaluated in patients with non-small cell lung cancer


Eligibility Criteria:
Inclusion Criteria:

* Patients with PD-L1-positive non-small cell lung cancer

  1. Age between 18 and 65 years old, gender is not limited.
  2. Patients with CT findings that can be occupied on the lung with a diameter greater than 1cm or more who have not undergone surgery.
  3. Lung cancer patients with a clinical diagnosis of positive PD-L1 expression;
  4. Written informed consent must be signed by the subject or his/her legal guardian or caregiver.
  5. Willingness and ability to cooperate with all programs of this study.

Exclusion Criteria:

* Subjects meeting any of the following criteria will be excluded from the study:

  1. Severe hepatic and renal insufficiency;
  2. Targeted therapy prior to radiotherapy or PET/CT scan. PD-L1 expression in existing lesions was assessed by immunohistochemistry using antibody clone 22C3. Renal function: serum creatinine less than or equal to the upper limit of the normal range;
  3. Liver function: bilirubin, AST(SGOT)/ALT(SGPT) less than or equal to the upper limit of the normal range.
  4. History of serious surgery in the last month.
  5. Those who have participated in other clinical trials during the same period.
Follow-up Study of Transcranial Direct Current Stimulation to Treat Major Depressive Disorder

The investigators purpose is to follow remitted patients from their previous study using transcranial direct current stimulation (tDCS); as to verify whether this treatment prevents relapse.

Our first ongoing study (Efficacy Study of Transcranial Direct Current Stimulation (tDCS) to Treat Major Depressive Disorder) addresses whether tDCS is an effective treatment for depression treatment. The purpose of the present study is to determine whether tDCS is also an effective strategy for sustaining remission.

We will enroll patients who presented remission using tDCS from our previous study and offer them to tDCS every other week for three months, than once a month for another three months. We will address whether tDCS prevents relapse by assessing depression scores once a month, using a time-to-event analysis.

The importance of the present study is to determine whether tDCS is able to sustain remission during long-term follow-up of depressed patients.

Eligibility Criteria:
Inclusion criteria:

Patients from previous studies (ID:USP-HU-001 and ID:USP-HU-002) who:

* received tDCS and responded.
* received tDCS + sertraline and responded.
* received sertraline or placebo and did not respond, and thereafter received tDCS during the cross-over phase and responded.

Exclusion criteria:

Patients from previous studies who:

* received tDCS and did not respond.
* received tDCS + sertraline and did not respond.
* received sertraline or placebo and responded.
* did not respond during the crossover phase.
* do not wish to participate in the present study.