Influence of Genetic and Physiological in Weight Loss

The study focuses on the influence of polymorphism in the FTO genes rs9939609 and PPARᵧ Pro12Ala, oxidative stress and systemic inflammation on changes in body composition and rest metabolism induced by HIIT and continuous aerobic programs in obese or overweight individuals.

Although the positive effects of continuous aerobic training are already well documented in the literature on health aspects. The weight loss induced by physical training is still very discrete when compared to other antiobesity means. Studies demonstrate an important variability in responses to physical exercise, indicating that individuals respond more or less to weight loss. Among these aspects, the factors that may influence exercise-induced weight loss have not yet been elucidated. Thus, the objective of the study is to analyze the influence of polymorphism in the genes FTO rs9939609 and PPARᵧ Pro12Ala, oxidative stress and systemic inflammation on changes in body composition and rest metabolism induced by continuous and continuous aerobic programs. Healthy individuals with overweight and / or obesity, aged 20-45 years, were randomly assigned to three groups: Continuous Aerobic (AC), Intermittent High Intensity Training (HIIT) and Control Group (CG). Groups (AC) and (HIIT) engaged in a 12-week program. Before the start of the program, at the sixth week and 48 hours after the intervention, they performed blood collections, ergospirometry, dual X-ray densitometry (DEXA), nutritional assessments. In addition, collection of buccal mucosa was performed for genotyping of polymorphisms studied. Initial differences between the groups, as well as between the dependent variables studied will be assessed using the two-way ANOVA test or its non-parametric equivalent. Correlation and regression tests will be performed to verify the influence of the independent variables and the magnitude of the exercise-induced weight loss, according to the normality of the data or differences between the standard deviations. The hypothesis tested is that genetic and physiological factors influence the weight loss independent of the training modality.

Eligibility Criteria:
Inclusion Criteria:

Men and women aged 20-45 years;

Were insufficiently active for at least six months prior to recruitment as determined by the International Physical Activity Questionnaire (IPAQ) (\<150 minutes of moderate to intense physical activity / week) (MATSUDO, 2001);

Were overweight or obese (BMI between 25kg / m2 and 39.9kg / m2) (WHO, 2015);

Did not suffer any change in weight (more than 5kg in the last three months);

Do not be smokers or acololistas (maximum of 2 doses / day);

They may not be users of supplements and / or medications that influence weight loss or weight gain;

No history of coronary, respiratory, metabolic, or musculoskeletal disease affecting ability to participate in the training program;

Have been considered fit in an exercise test performed immediately prior to physical intervention;

Women are not menopausal (menstrual cycle stopped more than one year) or have symptoms related to the climacteric phase.

Exclusion Criteria:

Individuals who lack at least 25% of the total training program or two consecutive weeks;

Initiate medication treatment, supplementation, diet and / or other physical training program during the intervention period;

During training, osseous and / or myoarticular lesions that affect the ability to continue participating in the intervention.