Ixmyelocel-T Treatment of Patients With Osteonecrosis of the Femoral Head

The purpose of this study is to determine if Ixmyelocel-T grafting with demineralized bone matrix bound in autologous plasma after core decompression surgery is superior to core decompression with demineralized bone matrix bound in autologous plasma in preventing progression of osteonecrosis to a more severe disease stage (Stage II to III or higher) from the time of surgery until 24 months later, in patients with University of Pennsylvania (UPenn) Stage IIB or C disease at diagnosis.

This study is an event-driven, multi-center, prospective, independent observer-blinded, controlled, randomized Phase III clinical trial enrolling patients diagnosed with University of Pennsylvania (Steinberg) Classification Stage IIB or C osteonecrosis of the femoral head.

* The first patient group is the Treatment Group and will receive core decompression and treatment with BRCs and demineralized bone matrix bound in autologous plasma, and
* The second patient group is the Control Group and will receive core decompression and demineralized bone matrix bound in autologous plasma, without any BRCs.
* Enrollment: With an anticipated drop-out rate of 10% in up to twenty (20) sites, a total of approximately 135 patients will be enrolled and randomized to obtain 120 evaluable patients (75 for the TRC treatment group and 45 for the Control treatment group).
* Primary endpoint: The percentage of patients progressing to a more severe UPenn disease stage (Stage II to III or higher) between 0 and 24 months will be the primary efficacy variable to demonstrate TRC therapy is superior to Control therapy.

Eligibility Criteria:
Inclusion Criteria:

* UPenn (Steinberg) classification of osteonecrosis, inclusive of Stages IIB and IIC. Diagnosis will be based on magnetic resonance imaging (MRI).
* Modified index of necrotic extent \< 40
* Idiopathic and non-idiopathic osteonecrosis.
* No infection in affected bones at the time of surgery.
* Patient competent to give informed consent.
* Normal organ and marrow function defined as:

  * Leukocytes ≥ 3000/µL;
  * Absolute neutrophil count ≥ 1500/µL;
  * Platelets ≥ 140,000/µL;
  * Serum AST (SGOT)/ALT (SGPT) \< 2.5 X institutional standard range;
  * Serum creatinine within normal limits, based on clinical laboratory normal range.
* Female patients not pregnant or lactating.
* Patients with a history of corticosteroids or on active therapy, will only be eligible for enrollment if corticosteroid use is suspended for 1 month prior and 6 months after cell therapy and surgery.
* Patients who have been treated with oral bisphosphonates are eligible for the trial if treatment was stopped at least 6 months prior to enrollment.

Exclusion Criteria:

* Stages IA, IB, IC, IIA, IIIA or more severe femoral head osteonecrosis, primarily based on diagnosis by MRI.
* Flattening of the femur head (UPenn Stage IV) or articular cartilage collapse at the time of core decompression surgery.
* Septic arthritis; stress fracture, or non-osteonecrosis metabolic bone diseases (e.g., Paget's disease of bone, osteogenesis imperfecta, primary hyperparathyroidism, fibrous dysplasia \[monostotic, polyostotic McCune-Albright syndrome\] and osteopetrosis).
* Any active bisphosphonate treatment or any history of intravenous (IV) treatment
* HIV, syphilis, positive at time of screening.
* Active hepatitis B or hepatitis C infection at the time of screening
* Known allergies to protein products (horse or bovine serum, or porcine trypsin).
* Patients who will require continuous, systemic, high dose corticosteroid therapy (more than 7.5 mg/day) within 6 months after surgery.
* Patients in active treatment for cancer or blood dyscrasia, or have received chemotherapy, radiotherapy or immunotherapy in the past 2 years.
* Immunodeficiency diseases.
* Participation in another clinical study in the past 30 days or concurrent participation in another clinical trial.
* History of regular alcohol consumption exceeding 2 drinks/day (1 drink = 5 oz \[150 mL\] of wine or 12 oz \[360 mL\] of beer or 1.5 oz \[45 mL\] of hard liquor) within 6 months of screening and/or history of illicit drug use.
* MRI-incompatible internal devices (pacemakers, aneurysm clips, etc)
* Body mass index (BMI) of 40 Kg/m2 or greater
* Patients unable to tolerate general anesthesia defined as an American Society of Anesthesiologists (ASA) criteria of \> 2
* Patients with poorly controlled diabetes mellitus (HbA1C \> 8%), or with peripheral neuropathy, or known concomitant vascular problems.
* Patients receiving treatment with hematopoietic growth factors or anti-vasculogenesis or anti-angiogenesis treatment
* Traumatic osteonecrosis