Comparative neuronal uptake and cytotoxicity of anti-Hu and anti-Ri antibodies in rat cerebellar and hippocampal slice cultures

WCN 2013 No: 1897 Topic: 36 — Other Topic Comparative neuronal uptake and cytotoxicity of anti-Hu and anti-Ri antibodies in rat cerebellar and hippocampal slice cultures J.E. Greenlee, S.A. Clawson, B. Wood, K.E. Hill, N.G. Carlson. Neurology Service, Veterans Affairs Medical Center, Salt Lake City, UT, USA; Neurology, University of Utah Health Sciences Center, Salt Lake City, UT, USA; Research Service, Veterans Affairs Medical Center, Salt Lake City, UT, USA; Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT, USA; Center on Aging, University of Utah School of Medicine, Salt Lake City, UT, USA Background: Anti-Hu and anti-Ri are paraneoplastic autoantibodies recognizing intracellular antigens present in essentially all neurons. At autopsy, brains of patients with anti-Hu antibody show neuronal destruction. In contrast, anti-Ri antibody is less clearly associated with neuronal death, and patients with anti-Ri antibodies may respond to treatment. We have demonstrated that anti-Yo antibodies, associated with paraneoplastic cerebellar degeneration, accumulate intracellularly in cerebellar Purkinje cells in slice cultures of rat cerebellum and that antibody accumulation is followed by cell death. The present study was conducted to determine whether anti-Hu and anti-Ri antibodies are also taken up by neurons and whether uptake of either antibody is cytotoxic. Objective: To evaluate neuronal uptake and cytotoxicity of anti-Hu and anti-Ri antibodies in slice cultures of rat cerebellum and hippocampus. Materials and methods: Rat cerebellar and hippocampal slice cultures were incubated with anti-Hu or anti-Ri antibodies and evaluated over time for antibody uptake and for cell death. Specificity of anti-Hu cytotoxicity was confirmed by adsorbing anti-Hu IgGwith recombinant HuD protein. Results: Anti-Hu and anti-Ri antibodies accumulated in cerebellar and hippocampal neurons. Anti-Hu antibodies produced cell death which was significantly reduced by adsorption of anti-Hu IgG with recombinant HuD protein. In contrast, neurons accumulating anti-Ri antibodies showed no evidence of cell death as compared to controls. Conclusions: Anti-Hu and anti-Ri antibodies entered and accumulated in cerebellar and hippocampal neurons. Anti-Hu antibody associated neuronal death involved reactivity with HuD protein. Anti-Ri antibody did not affect neuronal viability and may cause neuronal dysfunction rather than cell death. doi:10.1016/j.jns.2013.07.2239 Abstract — WCN 2013 No: 1902 Topic: 36 — Other Topic An evaluation of the risks involved in ischemic encephalopathy among Mongolian children T. Sosorburam, B. Batbayar. Anesthesiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Children Hospital, Ulan-Bator, Mongolia Background: Ischemic encephalopathy is one of the emerging issues among third world countries. Hypoxia seems to be the main cause of this problem, but many risk factors are associated in causation of hypoxic ischemic encephalopathy (HIE). The aim of our study was to evaluate these risk factors among Mongolian children. Methods: This study was carried out at 3 children's hospitals, 85 neonates diagnosed with HIE over a year. Efforts were made to evaluate the questionnaire including details on parity, pre and postnatal histories, and specially the events of birth from labour till delivery of placenta. Results: There was a remarkable decreased antenatal hospital visits and almost 52% reported that they visited just once or twice during the whole pregnancy period. In 21%, there was history of increased mother age above 40 years while 62% reported that some incidents of hypoxia occurred at the time of birth. 20% used drugs or medication during the course of pregnancy without the consultation of the physician.31 % had a history of prolonged 2nd stage of labour. 49% were delivered by unskilled birth attendants. Conclusion: The data clearly showed that there are risks that can be avoided easily and a leading cause of mental retardation can be prevented. Lack of health awareness and decreased consultation from physicians on time seems to be the bulk of the problem in addition to lack of trained birth staff and health facilities. Efforts should be made to educate the mothers of child bearing ages, and counsel them to make the antenatal hospital visits more frequent as possible. doi:10.1016/j.jns.2013.07.2240 Abstract — WCN 2013 No: 1907 Topic: 36 — Other Topic Different emotions and strong stimuli: How do we make choices? WCN 2013 No: 1907 Topic: 36 — Other Topic Different emotions and strong stimuli: How do we make choices? C.V.D.S. Vilharba, A.J. Godoy. Marketing, University Sao Judas, Sao Paulo, Brazil; Neurology, University City of Sao Paulo, Sao Paulo, Brazil Our understanding of the way the brain makes a decision is still incomplete. We decided to analyze the relationships between color and different emotions and sound and different emotions and their influences to make a choice. Forty-eight undergraduate students of marketing courses were interviewed. We presented 7 objects with different colors while they were listening to a “sad” song and again while listening to a “happy” song. In each case they had to choose one of them. Then we presented six identical objects associated with different sounds while listening to those songs. Forty-six percent of the students selected the red color during the sad moment (SM) and 40% during the happy moment (HM). None of them chose white during SM (however 10% selected white during HM). During HM the “forgotten” color was orange (2% of the students). Twenty-nine percent of the volunteers chose the object associated with the sound of a bomb during SM (the same sound was selected only by 12% during HM). Only 2% selected the sound of the wind (during SM or HM). Our results suggest that strong stimuli (visual or auditory) activate many neural circuits, making someone choose a red object or one associated with the sound of a bomb. Nevertheless a so called Abstracts / Journal of the Neurological Sciences e629 (2013) e629–e678 e645