cssi/SciSciGPT-SciSciNet huggingface dataset:
Gait and neuromuscular adaptations after using a feedback-based gait monitoring knee brace.

Purpose The purpose of this study was to evaluate the gait and neuromuscular effects of a feedback-based gait monitoring knee brace. The aim of this paper was to explore how training with this knee brace affected the gait pattern utilized, rate of loading (ROL), and proprioceptive acuity. Methods Fifteen healthy women wore this knee brace that provided audible feedback on gait kinematics for a 30-min training period. We performed pre- and post-gait analyses and proprioceptive acuity assessments to determine gait learning and adaptation with this training. Results Post-training significant changes were seen in the knee angle prior to and at initial contact and peak knee extensor, flexor, and adductor moments, which ultimately led to a reduced ROL experienced. Subjects also had improved proprioceptive acuity post-training. Interpretations Our results indicate that using a feedback-based gait monitoring knee brace can change the gait pattern by increasing the knee flexion angle during the swing to stance transition. Though there was an approximate 25% decrease in the ROL experienced, there was also an increase in the knee adductor moment. Future long-term studies are needed to further explore the positive and negative effects of feedback-based gait monitoring knee brace on individuals with a compromised knee joint, such as those post-anterior cruciate ligament injury.
Functional Properties of an Isolated αβ Heterodimeric Human Placenta Insulin-like Growth Factor 1 Receptor Complex

Treatment of human placenta membranes at pH 8.5 in the presence of 2.0 mM dithiothreitol (DTT) for 5 min, followed by the simultaneous removal of the DTT and pH adjustment of pH 7.6, resulted in the formation of a functional ..cap alpha beta.. heterodimeric insulin-like growth factor 1 (IGF-1) receptor complex from the native ..cap alpha../sub 2/..beta../sub 2/ heterotetrameric disulfide-linked state. The membrane-bound ..cap alpha beta.. heterodimeric complex displayed similar curvilinear /sup 125/I-IGF-1 equilibrium binding compared to the ..cap alpha../sub 2/..beta../sub 2/ heterotetrameric complex. /sup 125/I-IGF-1 binding to both the isolated ..cap alpha../sub 2/..beta../sub 2/ heterotetrameric and ..cap alpha beta.. heterodimeric complexes demonstrated a marked straightening of the Scatchard plots, compared to the placenta membrane-bound IGF-1 receptors, with a 2-fold increase in the high-affinity binding component. IGF-1 stimulation of IGF-1 receptor autophosphorylation indicated that the ligand-dependent activation of ..cap alpha beta.. heterodimeric protein kinase activity occurred concomitant with the reassociation into a covalent ..cap alpha../sub 2/..beta../sub 2/ heterotetrameric state. These data demonstrate that (i) a combination of alkaline pH and DTT treatment of human placenta membranes results in the formation of an ..cap alpha beta.. heterodimeric IGF-1 receptor complex, (ii) unlike the insulin receptor, high-affinity homogeneous IGF-1 binding occurs inmore » both the ..cap alpha../sub 2/..beta../sub 2/ heterotetrameric and ..cap alpha beta.. heterodimeric complexes, and (iii) IGF-1-dependent autophosphorylation of the ..cap alpha beta.. heterodimeric IGF-1 receptor complex correlates wit an IGF-1 dependent covalent reassociation into an ..cap alpha../sub 2/..beta../sub 2/ heterotetrameric disulfide-linked state.« less
Suspected pediatric ingestions: Effectiveness of immunoassay screens vs. gas chromatography/mass spectroscopy in the detection of drugs and chemicals

Rapid and accurate analytical testing can be of great value when determining treatment for pediatric patients suspected of ingesting an unknown chemical. Though often overlooked, gas chromatography/mass spectroscopy (GC/MS) can be a valuable resource in emergency toxicology testing. In a recent 24-month period (July 1999-June 2001), the Analytical Toxicology Laboratory at the University of Mississippi Medical Center, Jackson, MS, compared the results of GC/MS analysis to results obtained by immunoassay testing. The laboratory tested 139 urine samples referred for STAT toxicology testing from the hospital's Pediatric Emergency Department. All samples were tested in parallel using an immunoassay technique (EMIT) and GC/MS. With analysis by immunoassay, 17.3% of the samples were positive for a drug of abuse. The number of positive drug classes ranged from 0 to 2 per sample (mean 0.17 +/- 0.43) using immunoassay. With analysis by GC/MS, drugs were detected in 88.5% of the samples. The number of drugs detected ranged from 0 to 11 per sample (mean 2.2 +/- 1.8) with GC/MS. A total of 64 different pharmaceuticals were identified by GC/MS. This study shows that analysis by GC/MS offers the clinician a more comprehensive view into the exposure of the pediatric patient presenting with an unknown chemical ingestion.
Drug release from electric-field-responsive nanoparticles

We describe a new temperature and electric field dual-stimulus responsive nanoparticle system for programmed drug delivery. Nanoparticles of a conducting polymer (polypyrrole) are loaded with therapeutic pharmaceuticals and are subcutaneously localized in vivo with the assistance of a temperature-sensitive hydrogel (PLGA-PEG-PLGA). We have shown that drug release from the conductive nanoparticles is controlled by the application of a weak, external DC electric field. This approach represents a novel interactive drug delivery system that can show an externally tailored release profile with an excellent spatial, temporal, and dosage control.
Slime molds, ascidians, and the utility of evolutionary theory

This game is about discovering generality in biology. How so? Let’s give the face values on the cards biological interpretations. An ace represents genes and molecules underlying biosynthetic pathways of great antiquity, the kings mechanisms of transcription and translation, the queens regulatory or signaling molecules. The cards comprising the stacked deck correspond to events that arose early in the history of life. The conventional deck, with its deuces and treys, represent taxon-specific biological features, say, the morphologies of antelope horns, the variety of social insect castes, or the polyp polymorphisms of siphonophores. A winning hand is a general finding, one germane to all or a large fraction of extant organisms.
Chinese Conceptualization of Learning

Despite much research on human learning, little is known about cultural conceptions of learning. This study examined indigenous conceptions of learning in Chinese. Prototypical methods were employed to collect Chinese learning-related terms. Cluster analysis yielded a hierarchical structure of this lexicon. Most items centered around the term meaning "heart and mind for wanting to learn," which emphasizes seeking knowledge as a lifelong orientation, diligence, endurance of hardship, steadfastness, and concentration. Such learning aims at achieving breadth and depth of knowledge, its application, the unity of knowing and morality, and contributions to society. Implications for theories and empirical research on learning are discussed.
Rural surgical workforce and care of colorectal disease

Abstract Over the past several years, increasing attention has been focused on the challenges associated with surgical care delivery in rural settings. These challenges include the relative scarcity of surgeons in rural America, professional isolation, rural case mix, regionalization, and financial issues surrounding rural surgery. This article will summarize these issues and describe the implications for the care of patients with colorectal diseases in rural settings.
Response of plant species to coal-mine soil materials

A two-year experiment was conducted on the Black Mesa Coal Mine near Kayenta, Arizona to investigate the growth and establishment of seven plant species in unmined soil (undisturbed soil) and coal-mine soil (spoils). Natural rainfall (20 cm/yr) and natural rainfull plus sprinkler irrigation (50 cm/yr) were the irrigation treatments applied to each soil material.
Roles of steroid receptor coactivator (SRC)-1 and transcriptional intermediary factor (TIF) 2 in androgen receptor activity in mice.

Genetic disruption of the steroid receptor coactivator (SRC)-1 and transcriptional intermediary factor (TIF)2/SRC-2 in mouse resulted in distinctive mutant phenotypes. To quantify their roles in the function of androgen receptor (AR) transcriptional activity in vivo, we generated a unique transgenic AR-reporter mouse and analyzed the cell-specific contributions of SRC-1 and TIF2 to the activity of AR in mouse testis. Transgenic AR-luciferase and transgenic AR-lacZ mice harbor a recombinant mouse AR gene, ARGAL4DBD, which is functionally coupled with a upstream activation sequence-mediated reporter gene (AR activity indicator). After characterization of these mice in terms of AR function, we further derived bigenic mice by crossing AR activity indicator mice with the SRC-1-/- or TIF2+/- mutant mice. Analyses of the resultant bigenic mice by in vivo imaging and luciferase assays showed that testicular AR activity was decreased significantly in those with the TIF2+/- mutation but not in the SRC-1+/- background, suggesting that TIF2 serves as the preferential coactivator for AR in testis. Immunohistological analysis confirmed that AR and TIF2 coexist in mouse testicular Sertoli cell nuclei under normal conditions. Although SRC-1 concentrates in Sertoli cell nuclei in the absence of TIF2, nuclear SRC-1 is not able to rescue AR activity in the TIF2 mutant background. Interestingly, SRC-1 appears to negatively influence AR activity, thereby counterbalancing the TIF2-stimulated AR activity. Our results provide unique in vivo insights to the multidimensional cell-type-specific interactions between AR and coregulators.
Gemfibrozil and fenofibrate, Food and Drug Administration-approved lipid-lowering drugs, up-regulate tripeptidyl-peptidase 1 in brain cells via peroxisome proliferator-activated receptor α: implications for late infantile Batten disease therapy.

The classical late infantile neuronal ceroid lipofuscinosis (LINCLs) is an autosomal recessive disease, where the defective gene is Cln2, encoding tripeptidyl-peptidase I (TPP1). At the molecular level, LINCL is caused by accumulation of autofluorescent storage materials in neurons and other cell types. Currently, there is no established treatment for this fatal disease. This study reveals a novel use of gemfibrozil and fenofibrate, Food and Drug Administration-approved lipid-lowering drugs, in up-regulating TPP1 in brain cells. Both gemfibrozil and fenofibrate up-regulated mRNA, protein, and enzymatic activity of TPP1 in primary mouse neurons and astrocytes as well as human astrocytes and neuronal cells. Because gemfibrozil and fenofibrate are known to activate peroxisome proliferator-activated receptor-α (PPARα), the role of PPARα in gemfibrozil- and fenofibrate-mediated up-regulation of TPP1 was investigated revealing that both drugs up-regulated TPP1 mRNA, protein, and enzymatic activity both in vitro and in vivo in wild type (WT) and PPARβ−/−, but not PPARα−/−, mice. In an attempt to delineate the mechanism of TPP1 up-regulation, it was found that the effects of the fibrate drugs were abrogated in the absence of retinoid X receptor-α (RXRα), a molecule known to form a heterodimer with PPARα. Accordingly, all-trans-retinoic acid, alone or together with gemfibrozil, up-regulated TPP1. Co-immunoprecipitation and ChIP studies revealed the formation of a PPARα/RXRα heterodimer and binding of the heterodimer to an RXR-binding site on the Cln2 promoter. Together, this study demonstrates a unique mechanism for the up-regulation of TPP1 by fibrate drugs via PPARα/RXRα pathway.
An application of the two-stage bayesian method to estimate the outrage rate of oil-fired boilers

Abstract An application of the two-stage Bayesian method has been made to estimate the outage rate of oil-fired boiler electric power plants. The two-stage approach developed and applied here represents an improved numerical analysis procedure based on the LOTUS 123 software package available for the IBM-PC. Further, it is shown that plant-specific estimates of boiler outage rates can be calculated based on generic outage data for all US oil-fired boilers, in addition to a subpopulaton of ‘sister’ plants. It is recommended that the two-stage approach be applied to analyze other such plant-specific outage data, especially in view of the interest among electric utilities in the life extension of such units.
Changes in Hepatocyte NADH Fluorescence during Prolonged Hypoxia

Deprivation of oxygen reduces oxidative phosphorylation and rapidly causes an increase in cellular NADH which can be monitored by fluorimetry. Previous studies have established that increases in NADH fluorescence accurately reflect the impairment in oxidative phosphorylation which occurs during brief periods of acute hypoxia. However, the potential usefulness of fluorimetry for following longer, clinically relevant periods of ischemia has not been explored. We studied changes in NADH fluorescence in rat hepatocyte suspensions and in isolated-buffer-perfused rat livers during hypoxia (pO2 < 50 mm Hg) for periods as long as 180 min. NADH fluorescence of hepatocyte suspensions consistently increased by about 15% after 13 to 15 min of hypoxia and coincided with a marked decrease in tissue ATP levels. Reoxygenation after 15 or 30 min of hypoxia resulted in recovery of ATP and NADH with minimal loss of viability, as measured by trypan blue exclusion. After 60 to 180 min hypoxia, the initial increase in NADH fluorescence was followed by a progressive, irreversible decline which correlated with decreased cell viability. Similar changes in NADH fluorescence were observed in isolated-perfused rat livers in which NADH fluorescence was monitored at the liver surface with a fiberoptic probe. Hypoxia for 30 min had no effect on NADH fluorescence, but hypoxia for longer periods caused a steady increase in fluorescence after 45-60 min. When hypoxia was prolonged (120 or 180 min), fluorescence peaked after 60-75 min and then declined progressively to levels below baseline. The greatest decrease in fluorescence was seen after 180 min of hypoxia. These studies suggest that, during hypoxia, the initial increase in NADH fluorescence indicates early, potentially reversible hypoxic injury, while subsequent decreases in NADH fluorescence are indicative of irreversible injury. It may be possible to exploit this phenomenon to develop a noninvasive technique for determining irreversible injury due to ischemia.
Evaluating the strength of salt bridges: a comparison of current biomolecular force fields.

Recent advances in computer hardware and software have made rigorous evaluation of current biomolecular force fields using microsecond-scale simulations possible. Force fields differ in their treatment of electrostatic interactions, including the formation of salt bridges in proteins. Here we conducted an extensive evaluation of salt bridge interactions in the latest AMBER, CHARMM, and OPLS force fields, using microsecond-scale molecular dynamics simulations of amino acid analogues in explicit solvent. We focused on salt bridges between three different pairs of oppositely charged amino acids: Arg/Asp, Lys/Asp, and His(+)/Asp. Our results reveal considerable variability in the predicted KA values of the salt bridges for these force fields, as well as differences from experimental data: almost all of the force fields overestimate the strengths of the salt bridges. When amino acids are represented by side-chain analogues, the AMBER ff03 force field overestimates the KA values the least, while for complete am...
DIAGNOSIS AND MANAGEMENT OF SUPRA-ESOPHAGEAL COMPLICATIONS OF REFLUX DISEASE

Supra-esophageal reflux disease may be manifested in numerous ways, including reflux laryngitis, chronic cough, chronic sinusitis, and dental enamel loss. The mechanisms of pharyngeal and laryngeal reflux are not clearly defined, and standard reflux testing does not consistently demonstrate supra-esophageal reflux. The diagnosis is usually based on clinical suspicion when other causes of symptoms are not found and on the patient’s response to empiric acid suppression. With the development of triple-probe pH monitoring, through which pharyngeal pH can be assessed along with esophageal pH, the physician may now be able to demonstrate pharyngeal reflux in relation to patient symptoms. Therapy consists primarily of behavioral modification and aggressive acid suppression, although some alternative therapies exist.
The biologic basis for neuroblastoma heterogeneity and risk stratification.

Purpose of reviewNeuroblastoma serves as the paradigm for the clinical utility of tumor-specific biologic data for prognostication. This review will describe the genetic and biologic basis for the diverse clinical phenotypes observed in neuroblastoma patients. It will also discuss the current approa
Influence of co-matrix proton affinity on oligonucleotide ion stability in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

In this article we investigated the role organic base co-matrices play in reducing oligonucleotide fragmentation during analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The organic base co-matrix plays an important role in influencing the gas-phase behavior of desorbed oligonucleotides. No correlation was found between the solution pH values and the molecular ion stability of two model oligonucleotides. Instead, a direct correlation between the co-matrix proton affinity and the oligonucleotide molecular ion stability is seen. A co-matrix whose proton affinity is close to or greater than the proton affinity of the nucleobases can serve as a “proton sink. ” We propose that upon laser desorption/ionization, the co-matrix competes with the nucleobases of the oligonucleotide for additional protons from the matrix. When a co-matrix such as triethylamine is added, the co-matrix, rather than the oligonucleotide nucleobases, is the preferred site of proton transfer from the matrix. Titration of standard oligonucleotide matrices with several co-matrices of differing proton affinity demonstrates that the co-matrix mole fraction is an important factor in oligonucleotide molecular ion stability. When the mole fraction of the co-matrix approaches that of the matrix, nearly complete elimination of oligonucleotide fragmentation is seen. Control experiments utilizing pyridine, a co-matrix whose proton affinity is less than that of thymine or the phosphodiester backbone, demonstrate that the co-matrix plays an active role in oligonucleotide stabilization. Information on matrix:co-matrix interactions with these analytes should facilitate improvements in MALDI-MS of oligonucleotides.
Refractive Error and Visual Function Difficulty in a Latino Population

Objective To assess the association of correction of myopia, hyperopia, and astigmatism with self-reported near and distance visual function. Design Population based, cross-sectional study. Participants A random sample of 4272 Latinos older than 40 years of age from Arizona with both ophthalmic examination and questionnaire data, including answers to the National Eye Institute 25-item Visual Function Questionnaire (NEI VFQ-25). Methods Cases of refractive error (RE) were defined as subjects who met at least 1 of the following criteria in both eyes: myopia, sphere −0.5 diopters (D) or less; hyperopia, sphere 1.0 D or more; or astigmatism, cylinder 0.75 D or more. Uncorrected refractive error (URE) was defined as having RE and achieving 2 lines or more of improvement in visual acuity in both eyes after refraction. The association between RE category and near and distance vision difficulty (as measured by the NEI VFQ-25 near and distance vision subscores) was modeled with ordinal logistic regression, adjusting for age, gender, diabetes, hypertension, income, education, and acculturation. Main Outcome Measures Association between RE correction and near and distance vision difficulty by type of RE (myopia, hyperopia, and astigmatism). Results Of 4272 participants, 54% had RE. Forty-eight percent of these had myopia, 41% had hyperopia, and 11% had astigmatism. Fourteen percent of myopes, 21% of hyperopes, and 22% of astigmatics had URE. Subjects with myopia, astigmatism, and hyperopia were significantly more likely to report more distance vision difficulty than subjects without RE, regardless of whether they had correction (odds ratios [ORs], 1.7–3.7; P Conclusions In this population, correction of any type of RE is not entirely sufficient to restore distance visual function to the level of those without RE. More research is necessary to determine the reasons for this shortcoming. Financial Disclosure(s) The author(s) have no proprietary or commercial interest in any materials discussed in this article.
Genetic changes in breast cancer -consequences for therapy? ☆

Abstract The knowledge of specific genetic changes and their biological consequences is critical to an understanding of the natural history of breast tumors and the development of rational means to prevent and treat them. A number of genetic changes have been identified in breast tumors. Some of these involve specific genetic loci that directly contribute to one or more attributes of transformation, i.e., dysregulated proliferation and invasion, while other changes confer genetic instability that increases the possibility of acquiring subsequent, specific genetic lesions relevant to tumorigenesis. Many of these changes have been correlated with an adverse prognosis and attempts to integrate these in comprehensive multifactorial prognostic models are in progress. Initial clinical trials have also demonstrated the “proof of concept” of targeting specific genetic lesions for therapeutic purposes. In the not too distant future, we can envision the use of specific therapies for individual (or subgroups of) tumors based on the specific genetic changes that lead to their development, rather than the global approach of multiagent cytotoxic therapy that is in widespread use today.
INTERNATIONAL STUDY ON Artemia:1 XXI. INVESTIGATIONS INTO WHY SOME STRAINS OF Artemia ARE BETTER FOOD SOURCES THAN OTHERS. FURTHER NUTRITIONAL WORK WITH LARVAE OF THE MUD CRAB, Rhithropanopeus harrisii

In a series of studies conducted by members of the International Study on Artemia, differences in nutritional quality were documented between geographical strains of the brine shrimp Artemia. Concomitant with, and possibly related to nutritional effectiveness are differences in biometric traits and biochemical composition. Since these initial experiments could only hint at causal relationships, a series of experiments were carried out to further our understanding of cause and effect. In one experiment we tried to ascertain whether the factors responsible for poor survival in larvae fed some brine shrimp strains are cumulative throughout larval development or whether there are critical periods when larvae are more sensitive. Our results indicate that the factors contributing to poor growth and survival are cumulative. In a second experiment, we contaminated Brazil Artemia with diel-drin, chlordane and combinations of the two and fed them to mud crab (Rhithropanopeus harrisii) larvae. The levels of contamination were chosen to approximate those suspected of contributing to the poor food value of some brine shrimp strains. In this experiment, however, diel-drin and chlordane contamination appeared to have no effect on growth and survival of R. harrisii larvae. In a third experiment, we fed Utah strain Artemia on Isochrysis galbana for 24 hours and then fed these brine shrimp to mud crab zoeae. Previous work had shown that this regime made Utah Artemia more suitable as a food source for a prawn. However, in our experiments, it did not improve survival of R. harrisii.
International migration, non-traded goods and economic welfare in the source country

Abstract This paper examines the economic effects of emigration in a source country producing both traded and non-traded commodities. It is shown that, even if the economy faces fixed terms of trade, emigration can still affect the welfare of the non-migrants, and the direction of the effect in this context will always be negative.
Caveats when considering ketogenic diets for the treatment of pyruvate dehydrogenase complex deficiency

Abstract Objectives: We conducted a critical assessment of the use of diets high in fat and low in carbohydrate ("ketogenic") in the treatment of children with congenital lactic acidosis caused by mutations in the mitochondrial pyruvate dehydrogenase complex (PDC). Study design: The dietary composition of 18 subjects (11 from literature sources and 7 previously unpublished cases) was analyzed for nutrient composition. The biochemical and clinical responses to a long-term ketogenic regimen were also evaluated. Results: There was lack of uniformity in the proportion of fat calories administered and in the fatty acid composition of the diets. Ketogenic diets are also generally high in protein, compared with the recommended dietary allowance for age. Patient response to these regimens also varied considerably. Conclusions: Although ketogenic diets have become the standard of care for the treatment of PDC deficiency, data to support their use are based on a few uncontrolled case reports in which dietary composition varied widely. Furthermore, there are several theoretical reasons for concern about the long-term safety of high-fat, low-carbohydrate diets. A controlled, prospective evaluation of the risks and benefits of these regimens for patients with PDC deficiency is required to establish rational nutritional guidelines.(J Pediatr 2001;138:390-5)
Efficacy of Brief Quantitative Measures of Play for Screening for Autism Spectrum Disorders

Quick and effective screening measures are needed for detecting Autism Spectrum Disorder (ASD). Thirty typically developing children and 30 children with ASD aged 24–68 months were used. This study explored if the ASD group would exhibit less object exploration, diversity of play, and turn-taking than the typically developing group. Older children with ASD performed less turn-taking. On all other measures, IQ accounted for more of the difference between groups than diagnosis. Implications of these results for future research are discussed.
Explaining and Controlling Regression to the Mean in Longitudinal Research Designs.

This tutorial is concerned with examining how regression to the mean influences research findings in longitudinal studies of clinical populations. In such studies participants are often obtained because of performance that deviates systematically from the population mean and are then subsequently studied with respect to change in the trait used for this selection. It is shown that in such research there is a potential for the estimates of change to be erroneous due to the effect of regression to the mean. The source of the regression effect is shown to arise from measurement error and a sampling bias of this measurement error in the process of selecting on extreme scores. It is also shown that regression effects are greater with measures that are less reliable and with samples that are selected with more extreme scores. Furthermore, it is shown that regression effects are particularly prominent when measures of change are based on changes in dichotomous states formed from quantitative, normally distributed traits. In addition to a formal analysis of the regression to the mean, the features of regression to the mean are demonstrated via a simulation.
Inhibition of hypoglycemia-induced cortisol secretion by the serotonin antagonist cyproheptadine.

ABSTRACT Cortisol secretion is provoked by hypoglycemia. Since insulin-induced hypoglycemia is associated with an increase in hypothalamic serotonin, and since animal studies have related serotoninergic mechanisms to control of cortisol secretion, the role of hypothalamic serotonin in cortisol secretion was investigated in man. In normal volunteers, hypoglycemia-induced cortisol secretion was studied during a control period and after 2 days of administration of serotonin antagonists methysergide and cyproheptadine. Plasma cortisol was estimated by the competitive binding assay, and total cortisol secretion was expressed as the area under the cortisol curve from 0 to 120 min. Cyproheptadine administration resulted in a 31% reduction in the area under the cortisol curve, but methysergide administration did not result in a significant change. The plasma glucose levels in control insulin tolerance tests and the posttreatment tests indicated a comparable stimulus for cortisol secretion. If cyproheptadine acts ...
Belief, motivational, and ideological correlates of human rights attitudes.

Many people believe that an informed and thoughtful citizenry is essential to the maintenance of democratic ideals within the United States and the spread of those ideals abroad. Since the September 11, 2001, terrorist attacks, the evidence that Americans consider issues of human dignity and rights when making judgments about the U.S. government's war on terror has been mixed. In our study, we assessed the relative contributions of ideological, belief, and cognitive—motivational factors to the prediction of human rights and civil liberties attitudes. Individuals scoring high on measures of right-wing authoritarianism (RWA) and the belief that the structure of knowledge is simple were the most likely to support restrictions on human rights and civil liberties as part of the war on terror. In a subsequent regression analysis, individuals scoring higher on personal need for structure or exhibiting lower levels of epistemological belief complexity tended to score higher on RWA. Additionally, men were generall...
Control of surface mobility for conformal deposition of Mo–Si multilayers on saw-tooth substrates

Abstract Multilayer-coated blazed gratings (MBG) are the most promising solution for ultra-high resolution soft X-ray spectroscopy, since they can have very high groove density and provide high-order operation and very high diffraction efficiency. The performance of MBGs however depends critically on the conformal deposition of the multilayer (ML) stack on a saw-tooth substrate and the minimization of roughness. We present an analysis of the roughening and smoothing processes during growth of Mo/Si multilayers deposited over a range of pressures of Ar sputtering gas on flat and saw-tooth substrates. A Linear Continuum Model (LCM) of the film growth was used to understand the interplay between smoothing and roughening of the ML films and to predict the optimum conditions for deposition. The MBG coated under the optimal deposition conditions demonstrated high diffraction efficiency in the EUV and soft X-ray wavelength ranges
TRANSFORMING DNA INTEGRATES AT MULTIPLE SITES IN THE DIMORPHIC FUNGAL PATHOGEN BLASTOMYCES DERMATITIDIS

Abstract Blastomyces dermatitidis is a primary fungal pathogen of man and other mammals, but like many other human fungal pathogens, relatively little is known about the factors that account for its virulence and pathogenicity. We developed a transformation system to facilitate molecular genetic studies of putative virulence factors from B. dermatitidis. Transformation of the multinucleate yeasts was achieved by electroporation of DNAs containing a dominant selectable marker, hygromycin B (HygB) resistance. Southern analysis showed that transforming DNA invariably integrated ectopically into the chromosome. No evidence was found for extrachromosomal DNA. The HygB resistance could be expressed by either a 375-bp promoter fragment of the B. dermatitidis WI-1 gene encoding adhesin or an Aspergillus gpdA promoter placed 5′ of the E. coli hph gene. Primer extension analysis showed that for plasmids containing the WI-1 promoter, transcription of the hph gene initiated within the 375-bp WI-1 promoter fragment. The combination of gene transfer and two promoters capable of independent transcription will allow us to restore or augment gene expression in appropriate strains and test an influence on virulence. Molecular genetic manipulation of B. dermatitidis represents a major advance in our ability to investigate the pathogenesis of blastomycosis and other similar fungal diseases.
Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a degenerative disorder of motor neurons in the cortex, brainstem and spinal cord. Its cause is unknown and it is uniformly fatal, typically within five years. About 10% of cases are inherited as an autosomal dominant trait, with high penetrance after the sixth decade. In most instances, sporadic and autosomal dominant familial ALS (FALS) are clinically similar. We have previously shown that in some but not all FALS pedigrees the disease is linked to a genetic defect on chromosome 21q (refs 8, 9). Here we report tight genetic linkage between FALS and a gene that encodes a cytosolic, Cu/Zn-binding superoxide dismutase (SOD1), a homodimeric metalloenzyme that catalyzes the dismutation of the toxic superoxide anion O2.- to O2 and H2O2 (ref. 10). Given this linkage and the potential role of free radical toxicity in other neurodenegerative disorders, we investigated SOD1 as a candidate gene in FALS. We identified 11 different SOD1 missense mutations in 13 different FALS families.
Considering a multi-level approach to understanding maintenance of global coherence in adults with aphasia

Background: Discourse is a naturally occurring, dynamic form of communication. Coherence is one aspect of discourse and is a reflection of the listener's ability to interpret the overall meaning conveyed by the speaker. Adults with aphasia may present with impaired maintenance of global coherence, which in turn may contribute to their difficulties in overall communicative competence. Aims: The aim of the study was to determine if microlinguistic processes contribute to maintenance of global coherence in adults with and without aphasia. Method & Procedures: Participants included 15 adults with aphasia (PWA) and 15 healthy controls (HC). Study participants told stories conveyed in wordless picture books. The discourse samples were transcribed and then analysed for percent of information units produced, lexical diversity, syntactic complexity, and maintenance of global coherence. Outcomes & Results: Several linear regression models were carried out to investigate the relationship among the microlinguistic an...
Rethinking Civil Society in the Age of NAFTA: The Case of Mexico

This article offers an analysis and critique of the concept of civil society and its relationship to neoliberalism as an economic and political project. The author argues that the high level of imprecision in the usage of civil society has enabled both opponents and advocates of neoliberal policies to claim it. We forget that civil society must be understood as a stratified body whose members occupy specific class positions and, therefore, contend for control of vital resources. The author’s critique is largely based on the ignoring of class among those who study the intersection of neoliberalism and civil society. In the second part of this article, the author offers an illustration by focusing on Mexico’s recent history of democratization in tandem with the application of neoliberal policies.
Electronic and Magnetic Properties of Small Co-O Quantum Wires

Electronic and magnetic properties of small Co-O atomic clusters (“quantum wires”) have been studied in the framework of the Hartree-Fock (HF) method. The obtained results indicate that non-stoichiometric Co - O molecules with more than two O atoms possess at least one remarkably stretchable O-O bond that may facilitate significant re-construction of such molecules to larger structures. This re-construction may result in energetically favorable spin re-alignment in “antiferromagnetic” HF singlet Co-O molecules converting the singlets to larger “ferromagnetic” HF triplets and pentets. Such a spin re-alignment is energetically favorable, and may happen at the antiferromagnet-ferromagnet interface in “critical” core (Co)–shell (CoO) exchange-biased nanoclusters, providing for minimization of the surface energy, and leading to a loss of exchange bias. The obtained results are in agreement with available experimental and computational data.
Identification and Characterization of Small Molecule Inhibitors of a Class I Histone Deacetylase from Plasmodium falciparum

A library of approximately 2000 small molecules biased toward inhibition of histone deacetylases was assayed for antimalarial activity in a high-throughput P. falciparum viability assay. Active compounds were cross-analyzed for induction of histone hyperacetylation in a human myeloma cell line to identify HDAC inhibitors with selectivity for P. falciparum over the human host. To verify on-target selectivity, pfHDAC-1 was expressed and purified and a biochemical assay for pfHDAC-1 activity was established.
Global Attractor of Solutions of a Rational System in the Plane

We consider a two-dimensional autonomous system of rational difference equations with three positive parameters. It was conjectured that every positive solution of this system converges to a finite limit. Here we confirm this conjecture, subject to an additional assumption.
RESOURCE ALLOCATION: TOWARD A PRACTICAL SOLUTION

Abstract This paper presents a resource allocation algorithm for construction planning and control. The algorithm integrates the use of experience, decision-making criteria, a utility/cost matrix, an optimization procedure and a resource-smoothing heuristic. First, a planner uses his/her experience and the optimization procedure to develop several scheduling alternatives for reallocating a resource that is limited for a given interval (period-1) during the execution of a project. Next, the planner selects the best scheduling alternative by using the utility/cost matrix, where he/she can set performance goals and their relative importance according to his/her experience and company policy. Finally, the planner uses the optimization procedure and the resource-smoothing heuristic to reschedule the available resource efficiently within the remaining part of the project (period-2). The suggested method provides the user with a feasible solution that meets preset scheduling standards and planning criteria and a...
Impact of electronic surveillance on isolation practices.

Objective.To assess the impact of an electronic surveillance system on isolation practices and rates of methicillin-resistant Staphylococcus aureus (MRSA).Design.A pre-post test intervention.Setting.Inpatient units (except psychiatry and labor and delivery) in 4 New York City hospitals.Patients.All patients for whom isolation precautions were indicated, May 2009–December 2011.Methods.Trained observers assessed isolation sign postings, availability of isolation carts, and staff use of personal protective equipment (PPE). Infection rates were obtained from the infection control department. Regression analyses were used to examine the association between the surveillance system, infection prevention practices, and MRSA infection rates.Results.A total of 54,159 isolation days and 7,628 staff opportunities for donning PPE were observed over a 31-month period. Odds of having an appropriate sign posted were significantly higher after intervention than before intervention (odds ratio [OR], 1.10 [95% confidence interval {CI}, 1.01–1.20]). Relative to baseline, postintervention sign posting improved significantly for airborne and droplet precautions but not for contact precautions. Sign posting improved for vancomycin-resistant enterococci (OR, 1.51 [95% CI, 1.23–1.86]; P = .0001), Clostridium difficile (OR, 1.59 [95% CI, 1.27–2.02]; P = .00005), and Acinetobacter baumannii (OR, 1.41 [95% CI, 1.21–1.64]; P = .00001) precautions but not for MRSA precautions (OR, 1.11 [95% CI, 0.89–1.39]; P = .36). Staff and visitor adherence to PPE remained low throughout the study but improved from 29.1% to 37.0% after the intervention (OR, 1.14 [95% CI, 1.01–1.29]). MRSA infection rates were not significantly different after the intervention.Conclusions.An electronic surveillance system resulted in small but statistically significant improvements in isolation practices but no reductions in infection rates over the short term. Such innovations likely require considerable uptake time.
17O NMR spectra of 2‐substituted and 2,5‐di‐substituted furans. The inapplicability of the hammett LFER to correlate chemical shifts

The 17O NMR spectra of 27 2-substituted and 2,5-disubstituted furans are reported. The additivity of the 17O chemical shifts but the inapplicability of Hammett substituent constants to predict the observed shifts is demonstrated.
Age-associated changes in cardiovagal baroreflex sensitivity are related to central arterial compliance.

Cardiovagal baroreflex sensitivity (BRS) declines with advancing age in humans, but the underlying mechanism has not been established. Using two different approaches, we determined the relation bet...
The nature of the toxic reaction of influenza virus towards lung tissue.

Abstract The inoculation of large doses of unadapted influenza virus intranasally into mice results in the production of severe lung lesions. This toxic effect is a result of the entry of virus particles into the lung cells followed by uncoating of the virus ribonucleic acid. The toxic property of the virus is destroyed by procedures which destroy or modify the nucleic acid such as exposure to monochromatic UV light of wavelength 2537 A, or treatment with hydroxylamine or Bayer A139. Reagents acting on amino groups are particularly effective as they react with the nucleic acid and probably also interfere with penetration of virus into the cell. Toxicity is also destroyed by mercurials which probably prevent uncoating of the nucleic acid by union with disulphide bonds, and by oxidizing agents such as iodine, permanganate, osmic acid and hydrogen peroxide under condition which suggest possible action on some constituent of the virus containing methionine. The toxic effect produced by the inoculation of large doses of unadapted virus intranasally in mice is associated with the occurrence of an incomplete growth cycle in which there is full production of RNP antigen but no production of haemagglutinin or infective virus.
The fine structure of synapses in the ciliary ganglion of the chick.

Ciliary ganglia of chick embryos and newly hatched chicks were examined in the light and electron microscopes. Particular attention was given to the fine structure of calyciform synapses, which are characteristically found in ciliary ganglia of birds. The calyciform endings are characterized by large expansions of the presynaptic axons upon ganglion cells, and the terminal processes extend over a considerable area of the cell surface. Often, indeed they appear to envelop the cell. In the electron microscope image, the appositional membranes are separated by a space about 300 to 400 A wide; i.e., the synaptic cleft. At irregularly spaced regions, the appositional membranes show areas of increased density. The presynaptic processes contain clusters of synaptic vesicles, localized at these dense regions. Thus the fine structure complex typical of other synapses is evident. The unique structural features of this synapse are as follows: (a) The calyx or presynaptic terminal derives from a single axon, does not arborize, and terminates upon a single ganglion cell. Thus, unlike the classical bouton terminal, this represents an anatomical device for firing single cells by single axons. (b) The surface area in contiguity, i.e., the area of appositional membranes, is far more extensive than the bouton terminal. The fine structure of this synapse is compared with others, for example, the classical boutons terminaux and purely electrical synapses, in an attempt to correlate fine structure with function.
Characterizing the performance of an optical slip sensor for grip control in a prosthesis

Few commercially available prostheses have integrated force sensors for grip control. This paper introduces an optical-based sensor to detect a grasped object's displacement. The proposed sensor is commonly used as the sensing component in optical computer mice. The performance of the sensor was tested against objects with various optical properties to get a measure of slip detection accuracy for objects that an amputee may encounter on a daily basis. The sensor was found to accurately detect motion for the majority of the tested surfaces, with the exception of transparent surfaces or those with very high reflectivity. This research may help the development of a controlled prostheses that could assist an amputee in grasping an object for long periods of time without dropping it.
Intrauterine growth restriction and postnatal steroid treatment effects on insulin sensitivity in preterm neonates

OBJECTIVES: To study whether intrauterine growth restriction (IUGR) is associated with decreased sensitivity to the main fetal growth factor, insulin, and the effect of glucocorticoid therapy on insulin sensitivity in preterm infants. STUDY DESIGN: Newborn infants with a birth weight (BW) of< 1500 g were classified as appropriate for gestational age ([AGA], BW within +/- 1 SD, n = 10), or small for gestational age ([SGA], BW <-2 SD, n = 13); 5 AGA infants and 8 SGA infants received systemic steroids. An abbreviated modified minimal model test was performed, consisting of sequential blood samples for glucose and insulin assays, and intravenous infusions of 0.3 g/kg glucose and 0.02 U/kg regular human insulin. The insulin sensitivity index (S(I)) was calculated using a computer program. RESULTS: The basal insulin/glucose ratio (I/G) and S(I) did not differ between the AGA and SGA groups. Steroids did not influence the I/G nor the S(I) of AGA infants (10.2 +/- 6.7 vs 8.2 +/- 2.3), but decreased the S(I) in the SGA group (12.2 +/- 5.1 vs 5.3 +/- 2.7, P <.05). CONCLUSIONS: Insulin sensitivity of neonates can be measured by the modified minimal model. IUGR is not associated with impaired fetal glucose tolerance. Early neonatal steroid treatment decreases insulin sensitivity in SGA infants, which may contribute to their risk of having hyperglycemia. (Less)