cssi/SciSciGPT-SciSciNet huggingface dataset:
Adverse events during reoperative cardiac surgery: frequency, characterization, and rescue.

Objectives To (1) determine frequency of occurrence and risk factors for intraoperative adverse events (IAE) during reoperative cardiac surgery, (2) characterize them with respect to structure injured, timing, and use of preventive strategies, and (3) identify the impact on outcome in terms of successful and unsuccessful rescue and cost. Methods Operative notes of 1847 patients undergoing reoperative cardiac surgery were reviewed to identify and characterize documented intraoperative adverse events. Logistic regression modeling was used to identify risk factors for intraoperative adverse events and outcomes. Expected versus observed poor outcomes (stroke, myocardial infarction, death) was used to measure rescue. Results Among 127 patients, 145 (7%) intraoperative adverse events occurred. These included injuries to bypass grafts (n = 47), heart (n = 38), and great vessels (n = 28) and ischemia without graft injury (n = 22). Most occurred on opening (n = 34, 23%) and during prebypass dissection (n = 57, 39%). Risk incremented as reoperations increased. Seventy-seven patients experienced 1 or more lapses in preventive strategies. Patients with intraoperative adverse events had a greater number of poor outcomes (n = 24 [19%] vs n=107 [6.2%]; P P .0001), indicating 12 "failures to rescue." Conclusions Adverse events still occur regularly during cardiac reoperation, are related to complexity of the procedure, and occur particularly during dissection and often when preventive strategies have not been used. Compensatory rescue measures are not always successful. Adverse events lead to poor patient outcome and higher cost.
Helpful, Supportive and Sensitive: Measuring the Evaluation of Enacted Social Support in Personal Relationships

Several recent studies have sought to identify characteristics of better and worse attempts at support provision; however, there has been little explicit theoretical attention to the ways in which recipients evaluate enacted support. We developed a multidimensional scale to measure these evaluations. Study 1 asked 122 adults to interpret the meaning of three adjectives (helpful, supportive, sensitive) used in previous research. Although the meanings of these terms overlap, respondents associated helpful with problem-solving utility, supportive with relational assurance, and sensitivity with emotional awareness. Study 2 asked 396 students to rate a recalled conversation on 30 semantic-differential-type scales derived from Study 1. These ratings provided a basis for selecting 12 items to form valid and reliable scales of problem-solving utility, relational assurance, and emotional awareness. Study 3 provides independent validation of factor structure, scale reliability and validity, and conceptual distincti...
A Randomized Open Trial Assessing the Feasibility of Behavioral Activation for Pathological Grief Responding

This study investigated the feasibility of using behavioral activation to treat enduring postbereavement mental health difficulties using a two-arm, multiple baseline design comparing an immediate start group to a delayed start group at baseline, 12-, 24-, and 36-weeks postrandomization. Participants received 12-14 sessions of behavioral activation within a 12-week intervention period starting immediately after the first assessment or after 12weeks for the delayed start group. Prolonged grief, posttraumatic stress, and depression symptoms were assessed as outcomes. Compared with no treatment, behavioral activation was associated with large reductions in prolonged, complicated, or traumatic grief; posttraumatic stress disorder; and depression symptoms in the intent-to-treat analyses. Seventy percent of the completer sample at posttreatment and 75 percent at follow-up responded to treatment with 45 percent at posttreatment and 40 percent at follow-up being classified as evidencing high-end state functioning at 12-week follow-up.
A time course view of sentence priming effects.

Meaning activation was estimated during (standard naming) and after (delayed naming) target presentation to chart the time course of priming effects during reading comprehension. Using sentences biasing homographs toward their dominant and subordinate meanings, two experiments evaluated context effects across three naming-cue delays: immediate, baseline, baseline+600 ms all at a 0-ms interstimulus interval. When participants named a target immediately as it was presented, results converged with previous findings demonstrating initial context-sensitive meaning activation. The delayed naming conditions revealed little post-access influences for dominant contexts. Subordinate contexts, however, provided the strongest evidence of continued (or sustained) processing. It was concluded that context has immediate and automatic effects on initial meaning activation, after which, strategies are invoked for fine-tuning an interpretation of a sentence and integrating it with new information.
A general synthesis of long chain ω- and (ω-1)-hydroxy fatty acids

Abstract A method for the synthesis of long chain fatty acids substituted at the ω and ω-1 positions has been developed. The key step is the isomerization of the triple bond of an alkyn-1-ol from an internal position in the chain to the free terminus with a new, convenient reagent, sodium aminopropylamide (NaAPA). Standard functional group manipulations i.e., Jones oxidation, esterification and hydroboration of the triple bond are used to prepare ω-hydroxy fatty esters. The generality of the method is illustrated with syntheses of ω-hydroxy fatty esters with 24, 26, 28 and 30 carbon chains. In the 24 carbon series, hydration of the terminal triple bond of alkynoic ester 4a followed by reduction gave the (ω-1)-hydroxy ester.
T cells and cytokines in atherogenesis.

Recent findings suggest that inflammation plays a key role in atherosclerosis from the earliest stage of lesion initiation, to the ultimate complication of thrombosis. In patients who died because of acute coronary syndromes (ACS), coronary atherosclerotic plaques are characterized by the presence of macrophages, and to a lesser extent T-lymphocytes, at the immediate site of either plaque rupture or superficial erosion. The rupture-related inflammatory cells are activated, indicating ongoing inflammation. ACS patients are also characterized by activated circulating lymphocytes, monocytes and neutrophils, and by increased concentrations of proinflammatory cytokines and of the highly sensitive acute phase reactant C-reactive protein. Interestingly, an unusual subset of T cells, CD4+CD28null T cells, involved in vascular complication of rheumatoid arthritis because of their functional profile predisposing for vascular injury, are expanded in the peripheral blood and infiltrate the coronary lesions of ACS pat...
Comparison of diffusion approximation and Monte Carlo based finite element models for simulating thermal responses to laser irradiation in discrete vessels

Both diffusion approximation (DA) and Monte Carlo (MC) models have been used to simulate light distribution in multilayered human skin with or without discrete blood vessels. However, no detailed comparison of the light distribution, heat generation and induced thermal damage between these two models has been done for discrete vessels. Three models were constructed: (1) MC-based finite element method (FEM) model, referred to as MC-FEM; (2) DA-based FEM with simple scaling factors according to chromophore concentrations (SFCC) in the epidermis and vessels, referred to as DA-FEM-SFCC; and (3) DA-FEM with improved scaling factors (ISF) obtained by equalizing the total light energy depositions that are solved from the DA and MC models in the epidermis and vessels, respectively, referred to as DA-FEM-ISF. The results show that DA-FEM-SFCC underestimates the light energy deposition in the epidermis and vessels when compared to MC-FEM. The difference is nonlinearly dependent on wavelength, dermal blood volume fraction, vessel size and depth, etc. Thus, the temperature and damage profiles are also dramatically different. DA-FEM-ISF achieves much better results in calculating heat generation and induced thermal damage when compared to MC-FEM, and has the advantages of both calculation speed and accuracy. The disadvantage is that a multidimensional ISF table is needed for DA-FEM-ISF to be a practical modelling tool.
Electrostatic charge acceptance and decay of powder coating particles

Techniques have been developed to measure the acceptance and decay of electrostatic charge on a wide variety of powder coatings. The measurement of the initial charge carried by the powder was affected by the amount of powder deposited. The decay of charge occurred within four time scales: (1) 1 sec, electron loss to air and to the metallic substrate; (2) 1 to 5 sec, electron flow to neutralize induced positive charge; (3) 5 to 100 sec, rapid decay from powder; and (4) 1,000 to 10,000 sec, very long term decay. Since the decay of charge was markedly affected by moisture and composition, the decay properties can be controlled by these parameters. A mathematical model was constructed describing the quantitative charge decay during the time period of 100 to 10,000 sec. There was considerable heterogeneity within the powders with regard to charge acceptance and interaction with moisture. These measurement techniques give information concerning electrostatic effects on particle transport, adhesion, and polymer electrostatic behavior which are very important variables in a powder coating process.
Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives

Bacteriophage T4 initiates DNA replication from specialized structures that form in its genome. Immediately after infection, RNA-DNA hybrids (R-loops) occur on (at least some) replication origins, with the annealed RNA serving as a primer for leading-strand synthesis in one direction. As the infection progresses, replication initiation becomes dependent on recombination proteins in a process called recombination-dependent replication (RDR). RDR occurs when the replication machinery is assembled onto D-loop recombination intermediates, and in this case, the invading 3' DNA end is used as a primer for leading strand synthesis. Over the last 15 years, these two modes of T4 DNA replication initiation have been studied in vivo using a variety of approaches, including replication of plasmids with segments of the T4 genome, analysis of replication intermediates by two-dimensional gel electrophoresis, and genomic approaches that measure DNA copy number as the infection progresses. In addition, biochemical approaches have reconstituted replication from origin R-loop structures and have clarified some detailed roles of both replication and recombination proteins in the process of RDR and related pathways. We will also discuss the parallels between T4 DNA replication modes and similar events in cellular and eukaryotic organelle DNA replication, and close with some current questions of interest concerning the mechanisms of replication, recombination and repair in phage T4.
A Climatology of the Tropospheric Thermal Stratification Using Saturation Potential Vorticity

The condition of convective neutrality is assessed in the troposphere by calculating the saturation potential vorticity P* from reanalysis data. Regions of the atmosphere in which saturation entropy is constant along isosurfaces of absolute angular momentum, a state indicative of slantwise-convective neutrality, have values of P* equal to zero. In a global reanalysis dataset spanning the years 1970–2004, tropospheric regions are identified in which P* is near zero, implying that vertical convection or slantwise convection may be important in determining the local thermal stratification. Convectively neutral air masses are common not only in the Tropics but also in higher latitudes, for example, over midlatitude continents in summer and in storm tracks over oceans in winter. Large-scale eddies appear to stabilize parts of the lower troposphere, particularly in winter.
A petrographic history of martian meteorite ALH84001: Two shocks and an ancient age

ALH84001 is an igneous meteorite, an orthopyroxenite of martian origin. It contains petrographic evidence of two shock metamorphic events, separated by thermal and chemical events. The evidence for two shock events suggests that ALH84001 is ancient and perhaps a sample of the martian highlands. From petrography and mineral chemistry, the history of ALH84001 must include: crystallization from magma, a first shock (impact) metamorphism, thermal metamorphism, low-temperature chemical alteration, and a second shock (impact) metamorphism. Originally, ALH84001 was igneous, an orthopyroxene-chromite cumulate. In the first shock event, the igneous rock was cut by melt-breccia or cataclastic veinlets, now bands of equigranular fine-grained pyroxene and other minerals (crush zones). Intact fragments of the cumulate were fractured and strained (now converted to polygonized zones). The subsequent thermal metamorphism (possibly related to the first shock) annealed the melt-breccia or cataclastic veinlets to their present granoblastic texture and permitted chemical homogenization of all mineral species present. The temperature of metamorphism was at least 875 C, based on mineral thermometers. Next, Mg-Fe-Ca carbonates and pyrite replaced plagioclase in both clasts and granular bands, producing ellipsoidal carbonate globules with sub-micron scale compositional stratigraphy, repeated identically in all globules, The second shock event produced microfault offsets of carbonate stratigraphy and other mineral contacts, radial fractures around chromite and maskelynite, and strain birefringence in pyroxene. Maskelynite could not have been preserved from the first shock event, because it would have crystallized back to plagioclase. The martian source area for ALH84001 must permit this complex, multiple impact history. Very few craters on young igneous surfaces are on or near earlier impact features. It is more likely that ALH84001 was ejected from an old igneous unit (Hesperian or Noachian age), pocked by numerous impact craters over its long exposure at the martian surface.
Strategies to Develop Warm Solutions for Real-Time Pump Scheduling for Water Distribution Systems

An optimal pump operation schedule that maintains satisfactory hydraulics conditions can generally reduce energy consumptions compared to the traditional trial and error based pump operation schedule. Linking an evolutionary based optimization algorithm with a hydraulic simulation model has gained attention for obtaining the optimal schedule. However, this technique requires significant computation time and thus has difficulty in real-time implementation. This paper presents several tactics to generate warm solutions that can be used in the initial population of the evolutionary algorithms to reduce the computation times. Strategies to generate warm solutions include the use of linear programming, surrogate model known as machine learning or meta-model, and historical pump schedule for similar demand pattern. Providing warm solutions from approximate methods or previous day’s results to stochastic search methods can improve solution convergence and offers significant computation time benefits. Results obtained from different strategies are compared.
Service utilization and help seeking in a national sample of female rape victims.

Objective: Many women with mental health problems do not seek help. Despite substantial research on predictors of help seeking, little is known about factors associated with help seeking in at-risk populations (for example, rape victims). This study examined various forms of help seeking for emotional problems in relation to key variables in a national sample of female rape victims. Methods: Telephone interviews were conducted with a representative sample of 3,001 women (aged 18 to 76 years). All interviews were conducted between January 23, 2006, and June 26, 2006. Those endorsing a lifetime history of rape were included in the analyses presented here (N=556). Demographic characteristics, rape history, rape characteristics, psychopathology, and substance abuse were assessed. Help seeking was assessed by asking whether participants ever sought help for emotional problems, and if so, what types of services were sought (services provided through a medical professional, religious figure, or mental health professional). Results: Help seeking was endorsed by 60% of the sample. The final multivariable model showed that ever seeking help was associated with being white (OR=2.61), being married (OR=.42), and having posttraumatic stress disorder (OR=3.45). Specific forms of help seeking revealed unique predictor sets. Conclusions: Although lifetime history of help seeking among rape victims was high, 40% of respondents had never sought help for emotional problems. Odds of help seeking were generally increased by the presence of a mental disorder. The findings suggest that some respondents may have sought help from general medical professionals or religious figures for emotional problems related to being raped. Education and training regarding rape and associated disorders should be available to other professionals to support provision of care. Public policy should be strengthened regarding professionals’ service provision and should include reimbursement for mental health services so victims do not have to pay for services. (Psychiatric Services 59:1450–1457, 2008)
A statistical assessment of tropical cyclone activity in atmospheric general circulation models

The properties of tropical cyclones in three low-resolution atmospheric general circulation models (AGCMs) in seven ocean basins are discussed. The models are forced by prescribed, observed sea surface temperatures over a period of 40 yr, and their simulations of tropical cyclone activity are compared with observations. The model cyclone characteristics considered include genesis position, number of cyclones per year, seasonality, accumulated cyclone energy, track locations, and number of storm days. Correlations between model and observed interannual variations of these characteristics are evaluated. The models are found able to reproduce the basic features of observed tropical cyclone behavior such as seasonality, general location and interannual variability, but with identifiable biases. A bias correction is applied to the tropical cyclone variables of the three models. The three AGCMs have different levels of realism in simulating different aspects of tropical cyclone activity in different ocean basins. Some strengths and weaknesses in simulating certain tropical cyclone activity variables are common to the three models, while others are unique to each model and/or basin. Although the overall skill of the models in reproducing observed interannual variability of tropical cyclone variables has not surpassed or often even equalled that of statistical models, there exists potential for higher future skills using improved versions of dynamical approaches.
CULTURAL POLICY AS DEVELOPMENT POLICY: EVIDENCE FROM THE UNITED STATES

Cultural policies and cultural projects in the United States have been reframed to emphasize their economic benefits to cities. New alliances between arts advocates and place promoters are apparent at all levels, but are most prominent locally. These new alliances are facilitated by the changing interests of local officials and business people, who have come to believe there is economic value in the arts and of arts administrators, for whom attracting broader public support has become imperative. In some cities, entirely new organizational structures have sprung up to plan and implement projects that serve cultural and economic development advocates simultaneously. Such new institutions are most prominent in more economically disadvantaged cities.
Failure of nerve growth factor to enhance wound healing in the hamster.

We have investigated the effect of topically applied high molecular weight nerve growth factor (HMW-NGF) and 7S nerve growth factor (7S NGF) on healing of full-thickness skin wounds in the Syrian hamster. Our results indicate that HMW-NGF and 7S NGF have no significant influence on the rate of wound contraction or on the time to complete healing in this animal model.
Biological Warfare and Infection Control

Though many agents have been proposed as potential biological weapons, the feasibility of biological warfare is largely a matter of conjecture. The unpredictable and indiscriminate devastation caused by natural epidemics during wartime should warn us of the dangers of employing microbes as weapons.
Adolescent deviant peer clustering as an amplifying mechanism underlying the progression from early substance use to late adolescent dependence

BACKGROUND: Early substance use co-occurs with youths' self-organization into deviant peer groups in which substance use is central to social interaction. We hypothesized that the social dynamics of deviant peer groups amplify the risk of progressing from early use to later dependence, and that this influence occurs over and above escalations in use that typically accompany early substance use and membership in deviant groups. METHODS: Our study used a longitudinal, multimethod dataset consisting of 998 adolescents and their families. Participants were recruited from middle schools in a large metropolitan area in the Pacific Northwest. The sample was 47.3% female and ethnically diverse (42.3% European American, 29.1% African American, and 28.6% other, including biracial). We examined deviant peer clustering as a mediator between early substance use and later dependence, controlling for proximal levels of use, SES, early antisocial behavior, and parental monitoring. Tobacco, alcohol, and marijuana use were assessed at ages 12, 13, and 16-17. Past-year nicotine, alcohol, and marijuana dependence (DSM-IV) was assessed at age 19. Youth and parent reports and observational data were used to assess deviant peer clustering at age 16-17, and youth reported on antisocial behavior and parental monitoring at ages 12 and 13. RESULTS: Early substance use predicted increased likelihood of dependence on tobacco, alcohol, and marijuana by late adolescence. Deviant peer affiliation mediated these links, even when accounting for proximal levels of substance use. CONCLUSIONS: Early substance use not only promotes escalations in use across adolescence but also provides entry into a deviant social context that contributes to increased risk of dependence. Our results emphasize the importance of identifying and intervening in early substance use before it becomes an organizing factor in friendship selection and interaction. Deviant peer clusters are clearly an important avenue for intervention when seeking to interrupt the progression to substance dependence. Language: en
Transsphincteric pH profile at the gastroesophageal junction.

Background & Aims: Esophageal acid exposure conventionally is measured 5 cm above the lower esophageal sphincter (LES). The aim of this study was to compare pH profiles at sites within the LES, the distal esophagus, and the proximal stomach. Methods: Ten normal subjects underwent esophageal manometry followed by 24-hour esophagogastric pH monitoring using an 8-channel pH probe recording at 5 and 1.5 cm above and at 0, 1.5, 3.0, 4.5, 6.0, and 9.5 cm below the proximal LES border. During pH recording, a 4-hour gastric emptying test with an egg sandwich meal was performed. Results: The LES was 3.2 ± 0.4 cm in length. There was a progressive increase in acid exposure from the esophageal to the gastric pH sensors. pH was less than 4 for 3.4% ± 1.6%, 12.7% ± 8.5%, 26.5% ± 10.2%, 48.1% ± 11.3%, 66.5% ± 9.9%, 80.8% ± 5.6%, 89.2% ± 3.0%, and 96.7% ± 1.1% of the total time for pH probes at 5 and 1.5 cm above and 0, 1.5, 3, 4.5, 6.0, and 9.5 cm below the proximal LES border, respectively. Percentage acid exposures correlated significantly with the position of the probe (r = −0.95; P Conclusions: The percentage of recording time that pH was less than 4 was significantly higher in the intrasphincteric area and 1.5 cm above the proximal LES compared with the traditional site 5 cm above the proximal manometric LES border. High acid exposure in the intrasphincteric region might explain the susceptibility of the distal esophagus to erosions, strictures, and Barrett's esophagus.
Policy Based Data Placement in High Performance Scientific Computing

The recent increased use of workflow management systems by large scientific collaborations presents the challenge of highly dynamic data placement in distributed systems. Such data placement may be constrained by the enforcement of data placement policies. We present a Policy based Data Placement Service that enforces data placement policies, interfaces with workflow managers and improves workflow efficiency by offloading data staging jobs from workflows.
Stiffness of photocrosslinked RGD-alginate gels regulates adipose progenitor cell behavior

Adipose progenitor cells (APCs) are widely investigated for soft tissue reconstruction following tumor resection; however, the long-term success of current approaches is still limited. In order to develop clinically relevant therapies, a better understanding of the role of cell-microenvironment interactions in adipose tissue regeneration is essential. In particular, the effect of extracellular matrix (ECM) mechanics on the regenerative capability of APCs remains to be clarified. We have used artificial ECMs based on photocrosslinkable RGD-alginate to investigate the adipogenic and pro-angiogenic potential of 3T3-L1 preadipocytes as a function of matrix stiffness. These hydrogels allowed us to decouple matrix stiffness from changes in adhesion peptide density or extracellular Ca(2+) concentration and provided a physiologically relevant 3D culture context. Our findings suggest that increased matrix rigidity promotes APC self-renewal and angiogenic capacity, whereas, it inhibits adipose differentiation. Collectively, this study advances our understanding of the role of ECM mechanics in adipose tissue formation and vascularization and will aid in the design of efficacious biomaterial scaffolds for adipose tissue engineering applications.
Clinical outcomes in patients on preinjury ibuprofen with traumatic brain injury.

BACKGROUND: The aim of our study was to evaluate the clinical outcomes in patients on preinjury Ibuprofen with traumatic brain injury. METHODS: We performed a 2-year analysis of all patients on prehospital Ibuprofen with traumatic brain injury and intracranial hemorrhage. Patients on preinjury Ibuprofen were matched using propensity score matching to patients not on Ibuprofen in a 1:2 ratio for age, Glasgow Coma Scale, headabbreviated injury scale, injury severity score, International Normalized Ratio, and neurologic examination. Outcome measures were progression on repeat head computed tomography (RHCT) and neurosurgical intervention. RESULTS: A total of 195 matched (Ibuprofen 65, no-Ibuprofen 130) patients were included. There was no difference in the progression on RHCT (Ibuprofen 18% vs no-Ibuprofen 24%; P 5 .50). The neurosurgical intervention rate was 18.9% (n 5 37). There was no difference for need for neurosurgical intervention (26% vs 16%; P 5 .10) between the 2 groups. CONCLUSIONS: In a matched cohort of trauma patients, preinjury Ibuprofen use was not associated with progression of initial intracranial hemorrhage and the need for neurosurgical intervention. Preinjury use of Ibuprofen as an independent variable should not warrant the need for a routine RHCT scan. Published by Elsevier Inc.
Sex Differences in Self-Reported Beliefs About Foreign-Language Learning and Authentic Oral and Written Input

This study examined differences in the self-reports of men and women regarding their attitudes, beliefs, strategies, and experience in language learning. Nine hundred and thirtyeight students of Spanish at two large institutions responded to a questionnaire that had previously yielded 11 stable factors (Bacon & Finnemann, 1990). Through discriminant analysis, we could distinguish between the responses of men and women on two language-learning factors (LL), two dealing with authentic input (AI), and two exposure variables. The discussion includes a justification for using discriminant analysis as a statistical tool in second-language attitude and belief research.
Proangiogenic Cell Colonies Grown In Vitro from Human Peripheral Blood Mononuclear Cells

Although multiple culture assays have been designed to identify endothelial progenitor cells (EPCs), the phenotype of cells grown in culture often remains undefined. We sought to define and characterize the proangiogenic cell population within human peripheral blood mononuclear cells. Mononuclear cells were isolated from peripheral blood and grown under angiogenic conditions for 7 days. Formed colonies (CFU-As) were identified and analyzed for proliferation, mRNA and surface antigen expression, tube-forming ability, and chromosomal content. Colonies were composed of a heterogeneous group of cells expressing the leukocyte antigens CD45, CD14, and CD3, as well as the endothelial proteins vascular endothelial (VE) cadherin, von Willebrand’s factor (vWF), CD31, and endothelial nitric oxide synthase (eNOS). Colony cells expressed increased levels of proangiogenic growth factors, and they formed tubes in Matrigel. In comparison with colonies from the CFU-Hill assay, our assay resulted in a greater number of colonies (19 ± 9 vs. 13 ± 7; p < 0.0001) with a substantial number of cells expressing an endothelial phenotype (20.2% ± 7.4% vs. 2.2% ± 1.2% expressing eNOS, p = 0.0006). Chromosomal analysis indicated the colony cells were bone marrow derived. We, therefore, describe a colony-forming unit assay that measures bone marrow–derived circulating mononuclear cells with the capacity to proliferate and mature into proangiogenic leukocytic and endothelial-like cells. This assay, therefore, reflects circulating, bone marrow–derived proangiogenic activity.
In vivo effects of naproxen on composition, proteoglycan metabolism, and matrix metalloproteinase activities in canine articular cartilage

Naproxen is a nonsteroidal anti-inflammatory drug commonly used in the clinical treatment of joint disease. In this study, its effect in vivo on the biochemical composition, metabolic activities, and metalloproteinase activities of normal canine articular cartilage was analyzed. The articular cartilage from the knee joints of dogs who had been given naproxen for 4 weeks to maintain a serum level of 40-50 micrograms/ml was examined. Control animals were given a placebo. Treatment with naproxen was not found to change the composition (water, collagen, and proteoglycan) of the articular cartilage. The culture studies of cartilage explants indicated that proteoglycan synthesis rates were unaffected by the treatment with naproxen but that proteoglycan release from the tissue was suppressed. Analysis of the cartilage for matrix metalloproteinase activities showed reduced activity of neutral matrix metalloproteinase by 80%, of collagenase by 40%, and of gelatinase by 87%, with no change in activity of acid metalloproteinase or of tissue inhibitor for metalloproteinase. These findings indicate that in vivo treatment with naproxen has the capacity to modulate catabolic activities in articular cartilage.
In vitro antibacterial properties of magnesium metal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.

Abstract Bacterial infections are a costly sequela in any wound. The corrosion properties of 0.15, 0.30, 0.45 and 0.60 g of Mg metal were determined in Mueller–Hinton broth by serially measuring the Mg2+ concentrations and pH over 72 h. In addition, the effect of Mg metal, increased Mg2+ concentration and alkaline pH on the in vitro growth of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus were evaluated in three separate experiments. The primary outcome measure for culture studies was colony-forming units/ml compared to appropriate positive and/or negative controls. Regardless of the mass of Mg added, there was a predictable increase in pH and Mg2+ concentration. The addition of Mg and an increase of pH resulted in antibacterial effects similar to the fluoroquinolone antibiotic; however, a simple increase in Mg2+ concentration alone had no effect. The results demonstrate an antibacterial effect of Mg on three common aerobic bacterial organisms, the mechanism of which appears to be an alkaline pH.
Trusts, Taxes and Business: Dealing with 'Check-the-Box' Regulations

Is that trust an ordinary trust or a business trust? The tax man says it makes a difference. Business lawyers commonly assume that trusts formed for their clients will be taxed as ordinary trusts under federal income tax rules. This generally means trust income is taxed to the beneficiaries when trust income is actually distributed. When trust income is accumulated for later distribution, it is temporarily taxed to the trust itself and then later to beneficiaries who receive distributions and a form of tax credit for the tax paid earlier by the trust. These trust taxation norms do not apply when a trust is considered a business trust and is therefore taxed like other similar business entities. The applicable legal standard distinguishing an ordinary trust from a business trust has remained relatively static (albeit vague) since the ancient origins of our federal tax system. However, the consequence of business trust status was radically altered in 1997. In that year, the blockbuster check-the-box federal tax regulations mercifully mitigated the stakes of a trust being considered a business trust (the regulations were so designated because they allowed lawyers to choose tax classification simply by in effect checking the box relating to the most desired tax classification. See Treas. Reg. 301.7701-1 to -4).
Access Prediction for Knowledge Workers in Enterprise Data Repositories

The data which knowledge workers need to conduct their work is stored across an increasing number of repositories and grows annually at a significant rate. It is therefore unreasonable to expect that knowledge workers can efficiently search and identify what they need across a myriad of locations where upwards of hundreds of thousands of items can be created daily. This paper describes a system which can observe user activity and train models to predict which items a user will access in order to help knowledge workers discover content. We specifically investigate network file systems and determine how well we can predict future access to newly created or modified content. Utilizing file metadata to construct access prediction models, we show how the performance of these models can be improved for shares demonstrating high collaboration among its users. Experiments on eight enterprise shares reveal that models based on file metadata can achieve F scores upwards of 99%. Furthermore, on an average, collaboration aware models can correctly predict nearly half of new file accesses by users while ensuring a precision of 75%, thus validating that the proposed system can be utilized to help knowledge workers discover new or modified content.
Plasticity of Nanoporous Ni/YSZ Anode: A Numerical Analysis

Thin electrolytes (YSZ) and anode-supported (nanoporous Ni-YSZ ) cells operating in the temperature range of 650–850°C are considered as promising solid oxide fuel cell systems. Understanding the thermal-mechanical deformation behavior of the Ni/YSZ interface is critical for the design and durability assessment of the YSZ high temperature fuel cells. One of the problems still remains to be solved is the microstructure instability of the nanoporous Ni at the elevated temperatures. In this work, modeling the thermal-mechanical deformation in the nanoporous Ni/YSZ interface region was performed. Nanoporous Ni thin film bounded to YSZ was considered to establish a simplified 2-D model. On the Ni/YSZ interface, the nanopores are modeled as spherical or cylindrical pores. Crystal lattice rotation due to dislocation motion was simulated and the numerical solutions to the in-plane lattice rotation for the nanoporous Ni was used to predict the microstructure evolution in the interface area of the Ni/YSZ anode.© 2010 ASME
Evaluation of Column Performance in Liquid Chromatography

Afin de classer et selectionner une colonne chromatographique et la phase a employer, les proprietes chromatographiques sont etudiees: l'activite du silanol, l'hydrophobicite, l'activite du metal, celui-ci est incorpore sous forme de traces lors de la preparation de la silice. Le type de phase et la dimension des pores sont egalement analyses. Utilisation d'une colonne avec une phase stationnaire C18
Weight and Glucose Reduction Observed with a Combination of Nutritional Agents in Rodent Models Does Not Translate to Humans in a Randomized Clinical Trial with Healthy Volunteers and Subjects with Type 2 Diabetes.

Background Nutritional agents have modest efficacy in reducing weight and blood glucose in animal models and humans, but combinations are less well characterized. GSK2890457 (GSK457) is a combination of 4 nutritional agents, discovered by the systematic assessment of 16 potential components using the diet-induced obese mouse model, which was subsequently evaluated in a human study. Nonclinical Results In the diet-induced obese mouse model, GSK457 (15% w/w in chow) given with a long-acting glucagon-like peptide -1 receptor agonist, exendin-4 AlbudAb, produced weight loss of 30.8% after 28 days of treatment. In db/db mice, a model of diabetes, GSK457 (10% w/w) combined with the exendin-4 AlbudAb reduced glucose by 217 mg/dL and HbA1c by 1.2% after 14 days. Clinical Results GSK457 was evaluated in a 6 week randomized, placebo-controlled study that enrolled healthy subjects and subjects with type 2 diabetes to investigate changes in weight and glucose. In healthy subjects, GSK457 well tolerated when titrated up to 40 g/day, and it reduced systemic exposure of metformin by ~ 30%. In subjects with diabetes taking liraglutide 1.8 mg/day, GSK457 did not reduce weight, but it slightly decreased mean glucose by 0.356 mmol/L (95% CI: -1.409, 0.698) and HbAlc by 0.065% (95% CI: -0.495, 0.365), compared to placebo. In subjects with diabetes taking metformin, weight increased in the GSK457-treated group [adjusted mean % increase from baseline: 1.26% (95% CI: -0.24, 2.75)], and mean glucose and HbA1c were decreased slightly compared to placebo [adjusted mean glucose change from baseline: -1.22 mmol/L (95% CI: -2.45, 0.01); adjusted mean HbA1c change from baseline: -0.219% (95% CI: -0.910, 0.472)]. Conclusions Our data demonstrate remarkable effects of GSK457 in rodent models of obesity and diabetes, but a marked lack of translation to humans. Caution should be exercised with nutritional agents when predicting human efficacy from rodent models of obesity and diabetes. Trial Registration ClinicalTrials.gov NCT01725126
Immunochemical Characterization of Fetal Antigen Isolated From Spent Medium of a Human Melanoma Cell Line

A fetal antigen (FA) was isolated from spent culture medium of a melanoma (M14) cell line. Allogeneic serum samples from melanoma patients, previously characterized with respect to anti-FA activity, were used as the source of anti-FA antibody. The FA activity was partially purified by membrane ultrafiltration, gel filtration, and chloroform:methanol extraction. The partially purified FA was then used to develop an enzyme-linked immunosorbent assay (ELISA). By indirect ELISA both the IgG and IgM classes of anti-FA antibodies were detected in the sera of cancer patients and normal volunteers. The incidences of anti-FA antibodies in the sera of cancer patients and normal volunteers were not significantly different. As detected by competitive inhibition in ELISA, FA activity was widely distributed among melanoma, sarcoma, and carcinoma tumor tissues and cultured tumor cells, as well as among fetal brain, skin, and muscle tissues. FA activity was destroyed by treatment with beta-galactosidase and hyaluronidase, but it was not destroyed by proteolytic and lipolytic enzymes. The antigen bound to immobilized ricin, peanut, and soybean lectins. FA activity in material purified by ricin-affinity chromatography was associated with molecules in the 60,000- to 70,000-dalton region as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results suggest a glycoprotein nature for the FA isolated from the spent culture medium of melanoma (M14) cells; this FA apparently elicits formation of natural antibodies in the cancer patients and normal donors.
Physicians as role models.

The basic questions asked in the scenario are whether physicians should always “practice what they preach,” and whether a physician's personal life should be accessible and relevant to patients. Professional role models are important.
Phylogeography of the Antarctic planktotrophic brittle star Ophionotus victoriae reveals genetic structure inconsistent with early life history

In the marine environment, connectivity is influenced by physical oceanography as well as life history and behavioral traits, which in combination with historical events such as geology, physical oceanography, and climate, determine population structure. The Antarctic brittle star Ophionotus victoriae develops via a feeding planktonic larval stage, and therefore has potential for long-distance dispersal throughout its Antarctic/subantarctic range. To evaluate this hypothesis, phylogeography of this ecologically dominant species was elucidated by sequence analysis of two mtDNA genes from individuals collected throughout the Antarctic Peninsula and from two subantarctic islands. Counter to expectations of genetic homogeneity, mtDNA data revealed substantial levels of genetic differentiation as well as diversity. Although there were some genetically homogeneous populations, such as those throughout Bransfield Strait, we found O. victoriae to have significant population structure throughout much of the Antarctic Peninsula, with evidence of potential cryptic speciation between the western and eastern Antarctic Peninsula. Furthermore, Antarctic Peninsula populations were genetically distinct from subantarctic island populations. The low levels of connectivity implied for O. victoriae contrast with those found for many other Antarctic benthic taxa, and suggest a complex interplay between oceanography, recent climate history, and larval ecology.
Knowledge Conversion and Utilization

The purpose of this paper is to explore the conceptual and empirical literature on utilization with a view toward identifying and describing developments of theoretical significance.
Monitoring alveolar epithelial function in acute lung injury.

Objective.Identification of humoral markers of acute lung injury may lead to insights into pathologic mechanisms. In addition, specific markers may be useful for predicting development of acute respiratory distress syndrome (ARDS) or for assessing prognosis. Ultimately, studies of lung injury markers may help define interventions that prevent or moderate ARDS. The alveolar epithelium is important both for the integrity of the blood-gas barrier and for repair of the barrier after lung injury. This article reviews markers that derive from or relate to the alveolar epithelium and that might be used for monitoring alveolar epithelial function in acute lung injury. Surfactant apoproteins may be important markers of injury or for prognosis. Levels of surfactant apoprotein A (SP-A) fall 50–75% in patients with severe lung injury compared to normal patients. Serum levels of SP-A in patients dying of acute respiratory distress syndrome are double serum levels of survivors.
Rare Lesions of the Internal Auditory Canal

Background Approximately 95% of tumors occurring within the internal auditory canal (IAC) are vestibular schwannomas. Many undergo stereotactic radiation without definitive tissue diagnosis. Rare IAC tumors are not all radiosensitive and are poorly described. Methods Between 1992 and 2015, 289 consecutive patients with IAC lesions operated on were reviewed retrospectively. Results Fifteen patients (5.2%) (16 operations) had unusual histologic findings, including nonvestibular schwannomas (2 facial schwannomas, 2 cochlear schwannomas, 2 intermedius schwannomas), 3 meningiomas, 3 cavernous hemangiomas, a mucosa-associated lymphoid tissue lymphoma, an arachnoid cyst, and a lipochoristoma. None of these rare tumors could be identified before surgery. Three operative approaches were used: the retrosigmoid approach, middle fossa subtemporal approach, or translabyrinthine approach. Few complications occurred, including facial nerve palsy, loss of hearing, and vestibular function. Five-year average follow-up revealed one patient with recurrence. Conclusions Clinical examination and imaging alone were insufficient to correctly identify these tumors. Definitive pathologicdiagnosis should be strongly considered to help tailor treatment.
1.21 Experimental Mechanics of Composite Materials

Motivated by frequent inability of purely analytical or numerical tools to analyze complex problems in composites, this chapter discusses the applicability of experimental mechanic techniques to these materials. Thermoelastic, gray-field photoelastic, strain gage, moire, holographic, speckle, velocimetry, and high-speed photographic methods are emphasized. Most of these approaches enjoy the advantage of being full-field. Stress/strain/displacement analyses, vibrations, material characterization, fracture, fatigue, joints, and NDT are discussed, with applications to high-strength/-stiffness aerospace-type structural, cellulosic, carbon nano-tube, fiber-reinforced cement, asphalt and green (manure) composites.
Next-generation sequencing of non-small cell lung cancer using a customized, targeted sequencing panel: Emphasis on small biopsy and cytology.

Background: Next-generation sequencing (NGS) with a multi-gene panel is now available for patients with lung adenocarcinoma, but the performance characteristics and clinical utility of this testing are not well-described. We present the results of an extended 467 gene panel in a series of advanced, highly selected nonsmall cell lung cancer (NSCLC) patients using a range of specimens, including predominantly small biopsy and cytology specimens. Materials and Methods: A retrospective review of 22 NSCLC biopsies sent for NGS using an extended gene panel from January 2014 to July 2015. The customized NGS panel sequences 467 cancer-associated genes with exonic and intronic sequences obtained from purified tumor DNA. Genomic alterations, patient characteristics, and success of testing were determined. Results: The majority of samples tested were metastatic lung adenocarcinoma on final pathology. Of the 22 specimens tested, 5 (22.7%) were surgical resections and 17 (77.3%) were small biopsy and cytology specimens. Twenty-one (95%) of the specimens were adequate for full sequencing and yielded a total of 204 genomic alterations (average 8.9 per tumor), of which 17 (average 0.81 per tumor) were actionable and/or clinically relevant. Genomic alterations were found most commonly in the TP53, EGFR, EPHB1, MLL3, APC, SETD2, KRAS, DNMT3A, RB1, CDKN2A, ARID1A, EP300, KDM6B, RAD50, STK11, and BRCA2 genes. Conclusions: NGS using a comprehensive gene panel was performed successfully in 95% of all NSCLC cases in this series, including 94% small biopsy and cytology specimens and 100% surgical resections. This custom assay was performed on a range of tumor specimens and demonstrates that small specimens are able to provide a similar depth of information as larger ones. As many patients present at an advanced stage and only small specimens are obtained, the information these provide has the potential for guiding treatment in highly selected patients with advanced lung adenocarcinoma.