cssi/SciSciGPT-SciSciNet huggingface dataset:
Effects of Three‐Dimensional Culturing in a Fibrous Matrix on Cell Cycle, Apoptosis, and MAb Production by Hybridoma Cells

The effects of culturing hybridoma cells in a three-dimensional (3-D) poly(ethylene terephthalate) (PET) fibrous matrix on cell cycle, apoptosis, metabolism, and monoclonal antibody (MAb) production were evaluated by comparing with two-dimensional (2-D) culturing on microcarrier and multiwell plate surfaces. The percentage of cells in the G1/G0 phase increased during the long-term culturing period of ∼4 weeks. Compared to the 2-D culture systems, cells grown in 3-D matrices had higher MAb productivity for long-term culture. Decreasing serum content in the culture medium increased both MAb productivity and apoptosis. However, the 3-D culture had a greater increase in MAb productivity and a much lower apoptotic rate than the 2-D culture, especially at 0% serum. Most cells in the 3-D fibrous matrix formed large aggregates and were smaller than cells grown on a 2-D surface or in suspension. The smaller cell size allowed cells to survive better in the high-cell-density environment. The fibrous matrix also selectively retained healthy, nonapoptotic cells. These results suggested that the 3-D fibrous matrix contributed to growth arrest, protected cells to better resist low-serum environments, and reduced apoptosis, all of which contributed to the high viable cell density and volumetric MAb productivity in the long-term 3-D culture.
Finding the Office Funny

Alan Dundes and Carl R. Pagter. Sometimes the Dragon Wins: Yet More Urban Folklore from the Paperwork Empire. Syracuse, NY: Syracuse University Press, 1996. xv. 364 pp.
Induction of Polarity in Fucoid Zygotes.

Early events 'in the vegetative development of higher land plants are difficult to investigate because the young embryo is encased in the ovarian tissue of the previous gametophytic and sporophytic generations. Recently, genetic approaches have succeeded in identifying mutations that alter early development (Jurgens, 1995; see also Laux and Jurgens, 1997, in this issue), but cellular and physiological analyses of higher plant embryogenesis remain tedious. Brown algae of the family Fucales (comprising the genera Fucus and Pelvetia) are, however, very tractable for investigating early plant embryogenesis, especially the establishment of developmental polarity. These marine organisms grow attached to rocks in the intertidal zone and are typically 0.25 to 0.5 m in height at maturity. Zygotes, eggs, and sperm are shed from fronds of mature plants in the laboratory and can easily be harvested in gram quantities, free of other tissues. Young zygotes attach firmly to the substratum, and populations of adhering zygotes transit synchronously through early development. In the Fucales, mature eggs are radially symmetric, and cell polarization does not occur until after fertilization. The primary developmental axis is organized early in the first cell cycle and defines the growth axis of the young embryo. Unlike higher plants, in which positional cues are often conveyed by neighboring cells, fucoid zygotes develop autonomously and therefore rely on environmental signals for spatial information to orient their nascent axes. In the laboratory, an entire population can be induced to establish axes in parallel by the application of externa1 vectors such as unidirectional light (Jaffe, 1968). A few hours after polarization, localized growth occurs at one pole of the nascent axis, as shown in Figure 1. The first zygotic division is an invariant, asymmetric division, with the cell plate oriented transverse to the growth axis. The smaller rhizoid cell contains the growing apex and is the precursor to the holdfast of the alga; the larger thallus cell gives rise to the stipe and fronds of the mature plant (Figure 1). After the initial division of the zygote, the rhizoid cell elongates and repeatedly divides transverse to the growth axis, whereas the thallus cell undergoes proliferative divisions, each of which is transverse to the previous division.
ON THE “USE” OF NEOPRAGMATISM

. The present article continues an earlier critique of Robbins's and Rorty's neopragmatism. Their skepticism about the traditional concept of correspondence and about the criteria for truth are both unjustified, and their own assertion of meaning as usefulness either presupposes a prior notion of linguistic reference or fails to qualify as a sufficient criterion for knowledge. The difficulties with neopragmatism have implications for two other areas of the religion/science discussion, postmodernism and empirical Theology. Postmodernism shares neopragmatism's mistakes regarding the philosophy of language and can be rejected without endangering one's empiricism, humanism, or naturalism. By contrast, the strengths of empirical Theology, and of religious empiricism in general, can be preserved without Robbins's proposed ban on metaphysics.
Introduction of software engineering to freshman: Challenges and rewards

It is likely that the first programming course that a Software Engineering (SE) or a Computer Science (CS) freshman student takes at a university is Object- Oriented (OO) Programming with Java. The emphasis in the typical course is on development/language basics. The importance of process, design, testing, etc., considered core elements of Software Engineering, are typically ignored in such a class. It is believed that these concepts can be taught in later courses. This paper describes our experience of incorporating the core elements of the software engineering process in the first programming course for freshman at the Milwaukee School of Engineering (MSOE). The evolution of the introductory programming sequence at MSOE along with our initial assessment is also presented.
Photoinduced intercalation and coordination of a dirhodium complex to DNA: dual DNA binding.

Two new complexes, cis-H,H-[Rh2 (OCCH3 NH)2 (LL)(CH3 CN)2 ](2+) , where LL=bpy (2, bpy=2,2'-bipyridine) and dppz (3, dppz=dipyrido[3,2-a:2',3'-c]phenazine), were prepared from the reaction of cis-H,H-[Rh2 (OCCH3 NH)2 (CH3 CN)6 ](2+) (1) with the corresponding bidentate ligand. The bpy and dppz ligands chelate to the same rhodium atom and are positioned trans to the amidato N atoms, as determined by the single crystal X-ray structure of 2. Irradiation of 2 and 3 with visible light in water results in the exchange of one CH3 CNeq ligand for an H2 O molecule with quantum yields, Φ400 , of 0.040 and 0.044, respectively (λirr =400 nm). The identities of the photoproducts of 2 and 3 were determined to be cis-H,H-[Rh2 (OCCH3 NH)2 (L)(H2 O)(CH3 CN)](2+) , where L is bpy (4) and dppz (5), respectively. Mobility shift assays show that 4 crosslinks double-stranded DNA, and ESI-MS experiments indicate that both 4 and 5 form covalent adducts with single-stranded DNA. In addition, relative viscosity and 2D NMR experiments show that the dppz ligand of 5 also intercalates into DNA upon irradiation, making 3 a dual-binding agent that both intercalates and covalently binds to DNA upon the absorption of visible light.
An Historical Perspective on the Kissimmee River Restoration Project

This paper reviews the events leading to the channelization of the Kissimmee River, the physical, hydrologic, and biological effects of channelization, and the restoration movement. Between 1962 and 1971, in order to provide flood control for central and southern Florida, the 166 km-long meandering Kissimmee River was transformed into a 90 km-long, 10 meter-deep, 100 meter-wide canal. Channelization and transformation of the Kissimmee River system into a series of impoundments resulted in the loss of 12,000-14,000 ha of wetland habitat, eliminated historic water level fluctuations, and greatly modified flow characteristics. As a result, the biological communities of the river and floodplain system (vegetation, invertebrate, fish, wading bird, and waterfowl) were severely damaged. Following completion of the canal, the U.S. Geological Survey released a report documenting the environmental concerns associated with channelization of the river. This action led to the 1971 Governor's Conference on Water Management in South Florida that produced a consensus to request that steps be taken to restore the fish and wildlife resources and habitat of the Kissimmee basin. In 1976, the Florida Legislature passed the Kissimmee River Restoration Act. As a result, three major restoration and planning studies (first federal feasibility study [1978-1985], the Pool B Demonstration Project [1984-1990], and the second federal feasibility study [1990-present] were initiated (1) to evaluate measures and provide recommendations for restoring floodplain wetlands and improving water quality within the Kissimmee basin, (2) to assess the feasibility of the recommended dechannelization plan, and (3) to evaluate implementation of the dechannelization plan. The recommended plan calls for the backfilling of over 35 km of C-38, recarving of 14 km of river channel, and removal of two water-control structures and associated levees. Restoration of the Kissimmee River ecosystem will result in the reestablishment of 104 km 2 of river-floodplain ecosystem, including 70 km of river channel and 11,000 ha of wetland habitat, which is expected to benefit over 320 species of fish and wildlife.
Feasibility and safety of cCD20 RNA CAR-bearing T cell therapy for the treatment of canine B cell malignancies

CD19 chimeric antigen receptor (CAR) T cell therapy has shown great promise in treating human hematological malignancies. However, the full potential of CAR technology has not been reached in solid malignancies and limitations exist in utilizing genetically identical murine models to interrogate the safety and efficacy of anti-tumor immune therapies. Canines are an outbred population that naturally develop spontaneous disease. Here we have established a treatment model for CAR T cell therapy in outbred dogs with spontaneous B cell lymphoma. We developed a protocol to isolate, activate, and express CAR in canine T cells. We characterized a methodology utilizing anti-cCD3 antibody with a K562 antigen-presenting cell (APC) line that expresses human CD32 and canine CD86 to activate canine lymphocytes. This method yields robust (50-120x) expansion of cCD4+ or cCD8+ T cells from normal or lymphoma-diseased dogs. Canine T cells were then engineered utilizing mRNA electroporation to express a CAR targeting the B cell antigen CD20. An anti-canine CD20 (cCD20) CAR was selected for proof of principle studies to establish feasibility of efficient CAR expression and redirected effector function in canine T cells from a healthy and lymphoma-diseased dogs. The cCD20-Z CAR construct was efficiently, but transiently, expressed in canine T cells after mRNA electroporation. cCD20 CAR T cells exhibited specific lysis and IFN-γ secretion in response to cCD20+ target cells. These data indicate successful establishment of a method to isolate, activate, and express a fully functional CAR in canine T cells for use in adoptive immunotherapy. These results rationalized testing of this CAR approach in canines with advanced CD20+ malignancies. In an IACUC approved protocol, we administered three doses of autologous cCD20-Z RNA CAR+ T cells to a companion canine with advanced B cell lymphoma. Treatment was well-tolerated and no evidence of serious adverse events was observed. The anti-tumor effect, however, was transient, suggesting a need for product optimization. Future study will establish a lenti- or retroviral-based method to modify canine T cells to promote T cell persistence and durable anti-tumor effects in vivo. In conclusion, our study establishes the methodologies and feasibility of anti-cCD20 CAR therapy in canines with spontaneous B cell lymphoma that may represent a future clinical option for such companion pets. This work also lays the foundation for study of a large, outbred animal model that better resembles spontaneous, human malignancies to allow for improved assessment for potential toxicity risks prior to translation into human studies.
An Occupation in Harmony: The Roles of Markets and Government in Career Information and Guidance

Interest in career information and guidance (CIG) has grown as part of many countries’ efforts to strengthen education to improve their workforce. While CIG may facilitate markets in education and training, the provision of CIG itself is subject to market failures. This paper examines the demand for CIG, the structure of supply, and the nature of markets. Based on the OECD Career Guidance Policy Review, it then examines ways that countries have tried to correct such market failures in CIG – clarifying the responsibilities of governments to create the foundations necessary for markets.
Effect of Fuel Nozzle Configuration on Premix Combustion Dynamics

Combustion dynamics (or combustion oscillations) have emerged as a significant consideration in the development of low-emission gas turbines. To date, the effect of premix fuel nozzle geometry on combustion dynamics has not been well-documented. This paper presents experimental stability data from several different fuel nozzle geometries (i.e., changing the axial position of fuel injection in the premixer, and considering simultaneous injection from two axial positions). Tests are conducted in a can-style combustor designed specifically to study combustion dynamics. The operating pressure is fixed at 7.5 atmospheres and the inlet air temperature is fixed at 588K (600F). Tests are conducted with a nominal heat input of 1MWth (3MBTUH). Equivalence ratio and nozzle reference velocity are varied over the ranges typical of premix combustor design. The fuel is natural gas. Results show that observed dynamics can be understood from a time-lag model for oscillations, but the presence of multiple acoustic modes in this combustor makes it difficult to achieve stable combustion by simply re-locating the point of fuel injection. In contrast, reduced oscillating pressure amplitude was observed at most test conditions using simultaneous fuel injection from two axial positions.Copyright © 1998 by ASME
Comparison of driver models for the computer-aided analysis of electronic packages

A study of the different models available for the simulation of a driver/receiver circuit is discussed. The four models discussed are based on (1) devices, (2) look-up tables, (3) behavioral equations, and (4) simple RC equivalents. These models are tested for their suitability for analysis of driver/receiver circuits of electronic packages. The speed of computation, output accuracy, and delay calculations are tested and compared. >
Polyunsaturated fatty acids modulate the effect of TCF7L2 gene variants on postprandial lipemia

The transcription factor 7-like 2 (TCF7L2) has been recently associated with diabetes risk, and it may exert its effect through metabolic syndrome (MetS)-related traits and be subjected to modification by environmental factors. We investigated the effect of single nucleotide polymorphisms (SNP), rs7903146 and rs12255372, within the TCF7L2 locus on postprandial lipemia and other MetS-related traits and their modulation by dietary fat. Data were collected from 1083 European Americans participating in the Genetics of Lipid Lowering Drugs and Diet Network Study. Carriers of the minor T allele at the C/T rs7903146 SNP had higher fasting plasma glucose (P = 0.012), lower homeostasis model assessment of beta cell function (P = 0.041), higher plasma VLDL (P = 0.035), and lower large LDL particle (P = 0.007) concentrations and higher risk of MetS (P = 0.011) than CC individuals. Moreover, we identified significant interactions between this SNP and PUFA intake modulating fasting VLDL particle concentrations (P = 0.016) and postprandial triglycerides (TG) (P = 0.028), chylomicrons (P = 0.025), total VLDL (P = 0.026), and large VLDL (P = 0.018) concentrations. Thus, only T allele carriers with a PUFA intake > or = 7.36% of energy had elevated fasting plasma VLDL concentrations and postprandial TG-rich lipoproteins. These variables did not differ in T allele carriers and noncarriers in the low-PUFA intake group. Moreover, these significant interactions were due exclusively to (n-6) PUFA intake. In summary, high (n-6) PUFA intakes (> or = 6.62% of energy intake) were associated with atherogenic dyslipidemia in carriers of the minor T allele at the TCF7L2 rs7903146 SNP and may predispose them to MetS, diabetes, and cardiovascular disease.
Kinetics and microbial ecology of batch sulfidogenic bioreactors for co-treatment of municipal wastewater and acid mine drainage.

Abstract The kinetics and microbial ecology in sulfidogenic bioreactors used in a novel two-stage process for co-treatment of acid mine drainage (AMD) and municipal wastewater (MWW) were investigated. Michaelis–Menten modeling of COD oxidation by sulfate reducing bacteria (SRB) (Vmax = 0.33 mg L−1 min−1, Km = 4.3 mg L−1) suggested that the Vmax can be reasonably achieved given the typical COD values in MWW and anticipated mixing with AMD. Non-competitive inhibition modeling (Ki = 6.55 mg L−1) indicated that excessive iron level should be avoided to limit its effects on SRB. The COD oxidation rate was positively correlated to COD/sulfate ratio and SRB population, as evidenced by dsrA gene copies. Phylogenetic analysis revealed diverse microbial communities dominated by sulfate reducing delta-proteobacteria. Microbial community and relative quantities of SRB showed significant differences under different COD/sulfate ratios (0.2, 1 and 2), and the highest dsrA gene concentration and most complex microbial diversity were observed under COD/sulfate ratio 2. Major species were associated with Desulfovirga, Desulfobulbus, Desulfovibrio, and Syntrophus sp. The reported COD kinetics, SRB abundances and the phylogenetic profile provide insights into the co-treatment process and help identify the parameters of concerns for such technology development.
Local behavior of solutions of the stationary Schr\" odinger equation with singular potentials and bounds on the density of states of Schrödinger operators

ABSTRACTWe study the local behavior of solutions of the stationary Schrodinger equation with singular potentials, establishing a local decomposition into a homogeneous harmonic polynomial and a lower order term. Combining a corollary to this result with a quantitative unique continuation principle for singular potentials, we obtain log-Holder continuity for the density of states outer-measure in one, two, and three dimensions for Schrodinger operators with singular potentials, results that hold for the density of states measure when it exists.
Radichemical Purity and Stability of Generic Tc-99m DTPA

2740 Objectives Tc-99m diethylene triamine pentaacetic acid (DTPA) is utilized to evaluate Glomerular Filtration Rates (GFR). When using the modified Russell two-point method for GFR studies, the radiochemical purity (RCP) of Tc99m DTPA preparation must be greater than 98%. Methods Our laboratory evaluated the RCP and stability of low activity (101.3 mCi) and high activity (215.0 mCi) generic Tc-99m DTPA (Anazao Health) preparations at various time intervals ranging from 0.5 to 8.0 hours post formulation, using miniaturized chromatography consisting of Whatman 31ET strips with acetone and Agilant ITLC-SG strips with distilled water. At each specific time interval, three replicate chromatography strips were spotted, developed in the respective solution, cut at the specific cut line, and counted using a NaI(Tl) detector interfaced to a multichannel analyzer. The RCP data was statistically analyzed by calculating the mean and range. Results The initial radiochemical purity of the low activity Tc-99m DTPA preparation (101.3 mCi) was 99.1% and remained greater than 98.0% up to 8 hours post formulation. For the higher activity Tc-99m DTPA preparation (215.0 mCi), the initial radiochemical purity was 98.6%. The radiochemical purity remained greater than 98.0% for up to 4.5 hours after formulation. At 8 hour post formulation, the radiochemical purity of the high activity preparation was 97.2%, below the recommended purity necessary to perform the modified Russell two-point GFR study. Conclusions The radiochemical purity and stability of generic Tc-99m DTPA preparations were evaluated to comply to Russell two-point GFR studies. The RCP of Tc-99m DTPA preparations was greater than 98.0% up to 8.0 hours for low activity and up to 4.5 hours post formulation for high activity.
Television Content and Food Intake Among Restrained versus Unrestrained Eaters

There is a great deal of research suggesting that television (TV) viewing is associated with increased food intake. However, research on the relationship between TV content and food intake is limited. First, this study examined whether people ate more when TV content is related to food. Second, this study examined whether a TV program’s effect on food intake was moderated by people’s dieting status. Participants were asked to watch 30-minute TV programs that contained either food-related content or non-food-related content. While watching the TV programs, participants were allowed to eat two types of candy. The results indicated that there was no overall difference in candy intake based on the TV content; however, the association was moderated by their restrained eating status. Restrained eaters ate more candy while watching a food-related TV program while unrestrained eaters were not influenced by the content of the TV program.
The Perfect Firestorm: Bringing Forest Service Wildfire Costs Under Control

Prodded by a centralized planning and budgeting process, the U.S. Forest Service's expensive, one-size-fits-all approach to wildfire does not fit the extremely diverse 193 million acres of national forests. The agency's program - which consists of spending close to $300 million per year treating hazardous fuels and as much as $2 billion a year preparing for and suppressing fires - will not restore the national forests to health or end catastrophic fire in most of those forests. In many forests it may do more harm than good. Forest Service plans are based on the notion that western national forests suffer from an unnatural accumulation of hazardous fuels. In fact, that is probably true for no more than about 15 percent of those forests. The appropriate treatments on the remaining 85 percent may be as diverse as the forests themselves. Significant structural changes in the Forest Service are essential to control fire costs. They should decentralize decisionmaking so local decisions will respond to local economic and ecological conditions. This paper suggests several possible structural changes.
Control of leukemia driven by T315I escape mutations in BCR-ABL with yeast-based Tarmogen immunotherapy

C272 The emergence of targeted therapy-resistance mutations in BCR-ABL constitutes a significant vulnerability in the clinical treatment of chronic myelogenous leukemia (CML). The most prevalent escape mutation to CML treatment with imatinib mesylate (IM) or second generation tyrosine kinase inhibitor drugs (dasatinib and nilotinib) is a threonine-to-isoleucine mutation at codon 315 of BCR-ABL (T315I mutation). The goal of this study was to test whether yeast-based immunotherapy (TarmogenTM) targeting the T315I epitope triggers the specific elimination and control of leukemic cells driven by BCR-ABL harboring the T315I mutation. Since the BCR-ABLT315I epitope is a true tumor-specific antigen that is either not present or present at low levels prior to IM therapy, tolerance to this epitope should be low. Moreover, the immediate goal is not to eradicate the full leukemic burden, but rather to prevent the outgrowth of drug-resistant leukemic cells, thereby maintaining the effectiveness of current small molecule tyrosine kinase inhibitor therapies targeting the BCR-ABL kinase domain. Prior to testing the immunotherapeutic approach in a mouse leukemia challenge model, preliminary in silico and in vitro analyses of the feasibility of generating MHC class I T-cell epitopes encompassing the T315I mutation were performed. Overlapping 8-10 amino acid peptides spanning BCR-ABLT315I were analyzed using MHC-binding peptide prediction algorithms, synthesized and evaluated in an in vitro MHC-binding assay. Six candidate T315I H2-Kb binding peptides were identified. Recombinant S. cerevisiae baker’s yeast were then engineered to express ~300 amino acids of the mouse-specific version (2 amino acids different than human ABL sequence) of T315-mutated BCR-ABL (GI-10000 yeast). Mice challenged with 105 mouse leukemia cells whose proliferation is driven by BCR-ABL succumb ~10 days after challenge. Administration of GI-10000 yeast significantly extended survival in two different mouse strains upon challenge with leukemias harboring BCR-ABLT315I but not wild-type BCR-ABLWT. These results indicate that immune protection was targeted against the single amino acid alteration in the mutated BCR-ABL protein expressed by the leukemia cells. In confirmation of this result, the peripheral circulation of BCR-ABLT315I cells was reduced or eliminated in immunized, but not control, animals. Furthermore, after challenge with a mixed population of wild-type BCR-ABLWT and mutated BCR-ABLT315I leukemias, a more physiologically relevant model, the number of leukemic cells harboring BCR-ABLT315I was significantly reduced relative to cells expressing wild-type BCR-ABLWT in mice vaccinated with the GI-10000 yeast, but not control yeast. In summary, yeast-based immunotherapy targeting drug resistance or escape mutations represents a powerful approach to extend the clinical effectiveness of targeted cancer therapies.
The cervical and thoracic musculature of Lepidoptera: Part II. Comparison of published reports

Summary On the basis of all the published data, the 90 cervicothoracic muscle types found in the Lepidoptera are classified and their distribution among the various species is indicated. The muscles are grouped into 19 topographical categories under each of the four body regions: cervix, prothorax, mesothorax, and metathorax. A brief morphological discussion is given for each muscle type, and some information on its representation in other neuropteroid insects is included.
Assessing the Impact of Hand Motion on Virtual Character Personality

Designing virtual characters that are capable of conveying a sense of personality is important for generating realistic experiences, and thus a key goal in computer animation research. Though the influence of gesture and body motion on personality perception has been studied, little is known about which attributes of hand pose and motion convey particular personality traits. Using the “Big Five” model as a framework for evaluating personality traits, this work examines how variations in hand pose and motion impact the perception of a character's personality. As has been done with facial motion, we first study hand motion in isolation as a requirement for running controlled experiments that avoid the combinatorial explosion of multimodal communication (all combinations of facial expressions, arm movements, body movements, and hands) and allow us to understand the communicative content of hands. We determined a set of features likely to reflect personality, based on research in psychology and previous human motion perception work: shape, direction, amplitude, speed, and manipulation. Then we captured realistic hand motion varying these attributes and conducted three perceptual experiments to determine the contribution of these attributes to the character's personalities. Both hand poses and the amplitude of hand motion affected the perception of all five personality traits. Speed impacted all traits except openness. Direction impacted extraversion and openness. Manipulation was perceived as an indicator of introversion, disagreeableness, neuroticism, and less openness to experience. From these results, we generalize guidelines for designing detailed hand motion that can add to the expressiveness and personality of characters. We performed an evaluation study that combined hand motion with gesture and body motion. Even in the presence of body motion, hand motion still significantly impacted the perception of a character's personality and could even be the dominant factor in certain situations.
Time-Accurate Computations of Free-Flight Aerodynamics of a Spinning Projectile with Flow Control

This paper describes a new multidisciplinary computational study undertaken to compute the flight trajectories and simultaneously predict the unsteady free flight aerodynamics of a spinning projectile configuration both with and without aerodynamic flow control. Actual flight trajectories are computed using an advanced coupled computational fluid dynamics (CFD)/rigid body dynamics (RBD) technique. An advanced time-accurate Navier-Stokes computational technique has been used in CFD to compute the unsteady aerodynamics associated with the free flight of the spinning projectile at subsonic speeds. Computed positions and orientations of the projectile have been compared with actual data measured from free flight tests and are found to be generally in good agreement. The same advanced coupled procedure has been used to compute the aerodynamics of the spinning projectile with flow control using a synthetic microjet. Unsteady numerical results obtained from the coupled method show the flow field, the aerodynamic forces and moments, and the flight trajectories of the projectile.
Blood serum progesterone union β-HCG examination in ectopic pregnancy early time diagnosis and in treatment way choice value

Objective To discuss blood serum progesterone union β-HCG examination in ectopic pregnancy early time diagnosis and in treatment way choice value. Methods 45 example clinical doubtful ectopic pregnancy patient's blood serum progesterone, beta - the HCG level carries on the monitor, divided into 3 groups according to the pregnancy. Results Tubal pregnancy peritoneoscope surgery group 30 examples, MTX conservative treatment group 9 examples, anticipation treatment group 6 examples. Finally all 3 group of patient menopause time less than 49 days 12 examples, the blood serum progesterone mean value was 4.18±2.41ng/ml, β-HCG the mean value was 2690.35±1025.46mIU/L; The menopause time 49-70 day 27 examples, the blood serum progesterone mean value was 8.26±4.50ng/ml, β-HCG the mean value was 4660.90±1580.25mIU/L; The menopause time 71-91 day 6 examples, the blood serum progesterone mean value was 7.88±3.56ng/ml, β-HCG the mean value was 4126.44±1250.65mIU/L. The prompt blood serum progesterone and β-HCG the horizontal rush hours were located about the menopause 7-10 week; The tubal pregnancy laparoscope surgery group blood serum progesterone mean value was 8.98±3.58ng/ml, β-HCG the mean value was 5899.10±1988.00mlU/L; MTX conservative treatment group blood serum progesterone mean value for 2.45±1.08ng/ml, β-HCG mean value for 1285.20±554.24mlU/L; Anticipation treatment group blood serum progesterone mean value for 2.25±0.95ng/ml, β-HCG the mean value for 203.15±110.20mlU/L. The prompt laparoscope surgery group blood serum progesterone and β-HCG the value obviously was higher than the latter two groups (p0.05), medicine conservative treatment group and anticipation treatment group blood serum progesterone non-significance difference (p0.05), but β-HCG the value during three groups quite was even has the significance difference (p0.05). The blood serum progesterone didn't have the relevance with β-HCG the level and the ectopic pregnancy package of block size. Conclusions Blood serum progesterone examination simple, quickly, may take one kind of early diagnosis ectopic pregnancy the method, the union β-HCG examination has certain reference value to the ectopic pregnancy choice being suitable treatment way.
Influence of oxygenation in cyclic hydrocarbons on chain-termination reactions from R + O2: tetrahydropyran and cyclohexane

Abstract Multiplexed photoionization mass spectrometry (MPIMS) is used to determine branching fractions of conjugate alkenes from R + O2 oxidation reactions for cyclohexane (c-C6H12) and tetrahydropyran (c-C5H10O), a lignocellulosic-derived oxygenated biofuel. Because conjugate alkene formation is coincident with the formation of HO2, the results reveal the temperature- and pressure-dependent influence of the oxygen heteroatom on chain-termination propensity. The experiments were conducted using Cl-initiated oxidation at 10 and 1520 Torr and from 500 to 700 K, and ab initio calculations of bond energies, saddle point energies, and rate coefficients of unimolecular decomposition of α-tetrahydropyranyl were conducted to complement the experiments. Relative to the initial radical concentration [R]0 the trend in conjugate alkene branching fraction exhibits monotonic positive temperature dependence in both cyclohexane and tetrahydropyran, except for the latter species at 10 Torr where increasing the temperature to 700 K caused an appreciable decrease. The decrease in the branching fraction with increasing temperature in tetrahydropyran oxidation occurs because ring-opening rates of α-tetrahydropyranyl radicals, enabled by weak C–O bond dissociation energies, exceed rates of O2-addition. Ring-opening rate coefficients for α-tetrahydropyranyl, computed from stationary points at the CCSD(T)-F12a/cc-PVDZ//M06-2X/6-311++G** level of theory, confirm that ring-opening at 700 K is favorable and higher oxygen concentrations are required for O2-addition rates to be significant. With increased temperature and lower [O2], α-tetrahydropyranyl radicals preferentially undergo unimolecular decomposition into the linear radical C ˙ H 2 ( C H 2 ) 3 CHO . Conjugate alkene branching fractions measured at 1520 Torr for both cyclohexane and tetrahydropyran followed monotonic positive temperature dependence. The change in the tetrahydropyran trend at 1520 Torr relative to the 10 Torr measurements is ascribed to the increase in oxygen concentration mitigating ring-opening reactions of the initial R radicals. In contrast to the results at higher temperature, where ring-opening of tetrahydropyranyl radicals interrupts R + O2 chemistry and reduces the formation of conjugate alkenes, branching fractions measured below 700 K were higher in tetrahydropyran compared to cyclohexane at 10 Torr. The difference suggests that the ether group renders chain-termination pathways more-facile. Saddle point energy calculations on the surfaces of equatorial ROO conformers reveal that the barrier height to direct HO2 formation from α-tetrahydropyranylperoxy is lower by ca. 5 kcal/mol compared to cyclohexylperoxy at the CBS-QB3 level of theory, which facilitates low-temperature chain-termination.
Measurements of Kyy′(0°) Over A Resonance Region in 12C(d,n)13N

In the energy range from threshold to 12 MeV, the 12C(d,n)13N reaction and the mirror reaction 12C(d,p)13C exhibit considerable structure in the forward angle cross section excitation function. This structure must be due to compound nucleus effects in the interaction, so it is not surprising that DWBA calculations have never been able to describe suitably the angular dependence of the differential cross section, the polarization, and the asymmetry. In addition, the 12C(d,n)13N reaction has the interesting feature that although the magnitude of the differential cross section changes appreciably with energy in the region of the peaks in the excitation function, its angular dependence, or pattern, is not grossly altered. This observation, along with the fact that the reaction cross section exhibits an lp = 1 stripping shape, suggests that the effect of the resonances is primarily to enhance the probability that the stripping reaction should take place.
New strategies in removing non-coherency from signals with large distortion to noise ratios

Signal spectral analysis is a dominating way of characterizing the dynamic performance of analog signals. To perform accurate spectral testing, the IEEE Standard 1241, 1658 and 1057 suggest coherent sampling. Obtaining coherent sampling, especially when signals have large distortion to noise ratios, has been a challenge for many years. With the growing need for Built-In-Self-Test (BIST) circuits for lower test cost, achieving coherent sampling is becoming more and more challenging. For signal analysis, the signals under test don't usually have sufficient purity. On-chip oscillators, such as Wien Bridge oscillators and Ring oscillator don't possess high signal purity. For high speed, low resolution DACs, the output signal purity is also low. In that case, not only fundamental bin will have spectral leakage, harmonic bins will also have spectral leakages, which makes non-coherency harder to remove.
Pattern search in hierarchical high-level designs

The paper focuses on using algorithms for design pattern matching to address the challenges of designs at RT and higher abstraction levels. The crux of our approach is modeling designs and patterns as graphs, which lets us express design pattern matching as subgraph isomorphism. We apply a constraint-satisfaction approach and address the problem of both exact and generalized matching. Our experimental results confirm the applicability of our approach on industrial test cases.
Persistent Persister Misperceptions

ABSTRACT Persister cells survive antibiotic treatment due to their lack of metabolism, rather than through genetic change, as shown via four seminal experiments conducted by the discoverers of the phenotype (Hobby et al., 1942; Bigger, 1944). Unfortunately, over seven decades of persister cell research, the literature has been populated by misperceptions that do not withstand scrutiny. This opinion piece examines some of those misunderstandings in the literature with the hope that by shining some light on these inaccuracies, the field may be advanced and subsequent manuscripts may be reviewed more critically.
Concurrent panniculectomy in the obese ventral hernia patient: Assessment of short-term complications, hernia recurrence, and healthcare utilization.

Summary Background Panniculectomy (PAN) is often performed concurrently with ventral hernia repair (VHR) in the obese patient. However, the effectiveness and safety profile of this common practice are not fully established in part because of paucity of comparative effectiveness studies. In this study, a comparative analysis of early complications, long-term hernia recurrence, and healthcare expenditures between VHR-PAN and VHR-only patients is presented. Methods From the Healthcare Cost and Utilization Project database, obese patients who underwent VHR with and without concurrent PAN were identified. Multivariate cox proportional-hazards regression modeling was performed to compare outcomes between the two groups. Results The final cohort included 1013 VHR-PAN and 18,328 VHR-only patients. The VHR-PAN patients experienced a longer adjusted length of hospital stay (6.8 days vs. 5.2 days; p Conclusion Performing a concurrent PAN in the obese hernia patient is associated with a higher rate of early complications and greater healthcare expenditures, but overall a substantially lower incidence of 2-year hernia recurrence. The literature review presented here also highlights a substantial need for further comparative effectiveness studies to create the needed framework for evidence-based guidelines.
Tongue and Taste Organ Biology and Function: Homeostasis Maintained by Hedgehog Signaling.

The tongue is an elaborate complex of heterogeneous tissues with taste organs of diverse embryonic origins. The lingual taste organs are papillae, composed of an epithelium that includes specialized taste buds, the basal lamina, and a lamina propria core with matrix molecules, fibroblasts, nerves, and vessels. Because taste organs are dynamic in cell biology and sensory function, homeostasis requires tight regulation in specific compartments or niches. Recently, the Hedgehog (Hh) pathway has emerged as an essential regulator that maintains lingual taste papillae, taste bud and progenitor cell proliferation and differentiation, and neurophysiological function. Activating or suppressing Hh signaling, with genetic models or pharmacological agents used in cancer treatments, disrupts taste papilla and taste bud integrity and can eliminate responses from taste nerves to chemical stimuli but not to touch or temperature. Understanding Hh regulation of taste organ homeostasis contributes knowledge about the basic ...
Reproducing Cells Is Nothing New—A Historical Prospective

Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established.
Recovery of the bulk-like electronic structure of manganese phthalocyanine beyond the first monolayer on Bi2Te3

Abstract The evolution of electronic structure of manganese phthalocyanine on Bi2Te3 shows a transition to a bulk-like aspect abruptly after completion of the first layer. This allows the inference that, in the first layer, there is charge transfer and electronic hybridization involving the occupied Mn-derived d orbitals of the molecule into the conduction band of the substrate. The charge transfer coupling is seen using angle-resolved ultraviolet photoelectron spectroscopy by monitoring the evolution of work function and band structure with increasing molecular film thickness. The electronic structure in the second layer is more bulk-like as indicated by the reappearance of well-known low energy d orbitals that were depopulated in the first layer. Scanning tunneling microscopy shows that the transition to bulk like behavior is also reflected in film structure as a transition from a unique disordered monolayer to a locally ordered and dense second layer. These observations are relevant to ongoing efforts to control topological insulator interfaces especially for spintronics applications.
Development of hardwood seed zones for Tennessee using a geographic information system.

For species that have no or limited information on genetic variation and adaptability to nonnative sites, there is a need for seed collection guidelines based on biological, climatological, and/or geographical criteria. Twenty-eight hardwood species are currently grown for reforestation purposes at the East Tennessee State Nursery. The majority of these species have had no genetic testing to define guidelines for seed collection location and can be distributed to sites that have a very dzferent environment than that of seed origin(s). Poor survival and/orgrowth may result ifseedlings are not adapted to environmental conditions at the planting location. To address this problem, 30 yr of Tennessee county precipitation and minimum temperature data were analyzed andgrouped using a centroid hierarchical cluster analysis. The weather data and elevational data were entered into a Geographic Information System (GIs) and separately layered over Bailey's Ecoregions to develop a seed zone system for Tennessee. The seed zones can be used as a practical guideline for collecting seeds to ensure that the resulting seedlings will be adapted to planting environments. South. J. Appl. fit-. 27(3):172-175.
“An East, east of the East” Eça de Queirós’ A Relíquia, Álvaro de Campos’ “Opiary” and the Postimperial Scope of Portuguese Literary Orientalism

Coming to terms with the increasing peripherality of Portugal at the height of Europe’s “Scramble for Africa” and in its immediate wake, both Eca de Queiros and Fernando Pessoa’s Alvaro de Campos engage with orientalism reactively, setting the stage for a prescient critique of European representations of the Orient. Through the parody of nineteenth-century religious and scientific discourses (Eca), and of symbolist poetics (Alvaro de Campos), as well as the recontextualization of early-modern Portuguese travel writing tropes, these two writers propose two alternative understandings of Portugal’s specific position in the modern geopolitics of empire. This article argues that the prescience of Eca’s and Pessoa’s critiques of orientalism forecloses, rather than authorizes, future essentialist views of Portugal’s historical specificity as evidence of exceptionalism.
The Plk1 Piece of the Nuclear Envelope Disassembly Puzzle

Reporting in this issue of Developmental Cell, Linder et al. (2017) and Martino et al. (2017) reveal in highly complementary studies that Plk1 is recruited to the nuclear pore complex upon mitotic entry, where it acts with Cdk1 to hyperphosphorylate nucleoporin interfaces to promote NPC disassembly and nuclear envelope breakdown.
Abusive Supervision and Subordinate Self-Control: Implications for Social Exchange

We draw from social exchange and self-control theories to hypothesize that subordinates’ perceptions of abusive supervision are negatively associated with social exchange relationship quality with ...
Mouse RT6 Locus 1 and RAT RT6.2 are NAD

We report that rat RT6.2 and recombinant mouse Rt6 locus 1 proteins possess autoADP-ribosyltransferase activity and that Rt6, but not RT6, catalyzes the ADP-ribosylation of exogenous histones. Based on NH2OH sensitivity, it appeared that the ADP-ribose was attached to arginine residues on proteins. We also observed that the NAD+ concentration in culture medium correlates inversely with the proliferation of rat RT6+ T cells. The data suggest that lymphocyte surface ADP-ribosyltransferases could be involved in signaling and immunoregulatory processes.
Numerical analysis of the freeze-thaw performance of cementitious composites that contain phase change material (PCM)

Abstract We employ a numerical methodology to predict the freeze-thaw performance of cementitious composites containing lightweight aggregates (LWAs) impregnated with phase change material (PCM) as thermal energy storage (TES) agent. The governing equation for heat transfer is combined with homogenization techniques to predict the temperature and associated heat flow for cementitious materials during the phase transition of the PCM. The material properties of the cementitious composite and heat dissipation behavior during thermal cycling were characterized using a small-scale longitudinal guarded comparative calorimeter (LGCC) test. Phase transitions associated with freezing/melting of PCM occurs gradually over a narrow temperature range. Pore size effect of LWA on freezing and melting behavior of PCM was found to be relatively small. This approach is extended to the prediction of the thermal energy storage capacity of PCM on improving the freeze-thaw performance of concrete specimens exposed to realistic thermal conditions at various locations in the United States.
Structure Versus Stochasticity—The Role of Molecular Crowding and Intrinsic Disorder in Membrane Fission

Abstract Cellular membranes must undergo remodeling to facilitate critical functions including membrane trafficking, organelle biogenesis, and cell division. An essential step in membrane remodeling is membrane fission, in which an initially continuous membrane surface is divided into multiple, separate compartments. The established view has been that membrane fission requires proteins with conserved structural features such as helical scaffolds, hydrophobic insertions, and polymerized assemblies. In this review, we discuss these structure-based fission mechanisms and highlight recent findings from several groups that support an alternative, structure-independent mechanism of membrane fission. This mechanism relies on lateral collisions among crowded, membrane-bound proteins to generate sufficient steric pressure to drive membrane vesiculation. As a stochastic process, this mechanism contrasts with the paradigm that deterministic protein structures are required to drive fission, raising the prospect that many more proteins may participate in fission than previously thought. Paradoxically, our recent work suggests that intrinsically disordered domains may be among the most potent drivers of membrane fission, owing to their large hydrodynamic radii and substantial chain entropy. This stochastic view of fission also suggests new roles for the structure-based fission proteins. Specifically, we hypothesize that in addition to driving fission directly, the canonical fission machines may facilitate the enrichment and organization of bulky disordered protein domains in order to promote membrane fission by locally amplifying protein crowding.