cssi/SciSciGPT-SciSciNet huggingface dataset:
Galectin-3 is an important mediator of VEGF- and bFGF-mediated angiogenic response

Recent studies have shown that a carbohydrate-binding protein, galectin-3, is a novel pro-angiogenic molecule. The mechanism by which galectin-3 promotes angiogenesis remains unknown. We demonstrate here that galectin-3 is a mediator of vascular endothelial growth factor (VEGF)- and basic fibroblast growth factor (bFGF)-mediated angiogenic response. Angiogenesis assays revealed that galectin-3 inhibitors, beta-lactose and dominant-negative galectin-3, reduce VEGF- and bFGF-mediated angiogenesis in vitro and that VEGF- and bFGF-mediated angiogenic response is reduced in galectin-3 knockdown cells and Gal3(-/-) animals. Integrin alphavbeta3 was identified as the major galectin-3-binding protein and anti-alphav, -beta3, and -alphavbeta3 integrin function-blocking antibodies significantly inhibited the galectin-3-induced angiogenesis. Furthermore, galectin-3 promoted the clustering of integrin alphavbeta3 and activated focal adhesion kinase. Knockdown of GnTV, an enzyme that synthesizes high-affinity glycan ligands for galectin-3, substantially reduced: (a) complex N-glycans on alphavbeta3 integrins and (b) VEGF- and bFGF-mediated angiogenesis. Collectively, these data suggest that galectin-3 modulates VEGF- and bFGF-mediated angiogenesis by binding via its carbohydrate recognition domain, to the GnTV synthesized N-glycans of integrin alphavbeta3, and subsequently activating the signaling pathways that promote the growth of new blood vessels. These findings have broad implications for developing novel, carbohydrate-based therapeutic agents for inhibition of angiogenesis.
Technical and Radiotherapeutic Considerations of Intra-Oral Cone Electron Beam Radiation Therapy for Head and Neck Cancer.

I ntra-oral cone (IOC) electron beam radiation therapy for selected carcinomas of the head and neck is a highly effective radiotherapeutic modality and is primarily employed to boost the primary tumors in the oral cavity to a high-dose level while sparing the mandible, teeth, gums, and salivary glands, with consequent reduction in radiation sequelae yet with good local tumor control. The conventional treatment setup at the Massachusetts General Hospital about 25 years ago used a 280-kVp machine (Fig I). In 1978, the installation of a Varian Clinac 18 linear accelerator (Varian Assoc., Palo Alto, CA) made it possible to use electron beams with energies between 6 and 18 MeV. For IOC irradiation, electron beams have certain advantages over 250-kVp photon radiation. These include a relatively well-defined depth penetration related to the electron energy and a reasonable dose homogeneity of about 20%. Dose inhomogeneity resulting from angulation of the cone relative to the surface under treatment is not as serious as with photon beams, since the isodose contours generally follow the slope of the surface. The IOC system’ using electrons has been in operation for about 13 years and has been used mostly for treatment of selected carcinomas of the oral cavity.
Body size and the neural control of movement

Summary Nervous systems, muscles, and motor organs (biomechanics) generate movement cooperatively and should therefore change coordinately as body size changes. Proving this hypothesis requires a description of the nervous system, muscle, and biomechanics of individual species and comparison of these properties across a collection of species spanning a large size range. Obtaining these data in even one species is daunting, and we are far from reaching a general theory of how nervous system, muscle, and biomechanics depend on body size. Sufficient cross-species data nonetheless exist to identify some consequences of body size on the neural control of movement, which form the focus of this Primer: for example, locomotor cycle period depends on leg length; large animals should devote more neural resources to hazard avoidance and stride correction; the importance of gravity and momentum increases, and of passive muscle properties decreases, with limb size; and the medium in which movements occur affects these size dependencies. These considerations suggest that, in applying biological data to robotic design, proper ‘size' should be maintained across all levels (i.e., if small-animal neural mechanisms are used, so should small-animal muscle and biomechanical properties).
INTERNATIONAL LIQUIDITY AND AMERICAN POLICY

SUMMARY Two important themes in recent discussions of the evolution of international monetary institutions are the problems of cooperation among central banks and the role of exchange guarantees. These themes are illustrated by a discussion of United States Federal Reserve and Treasury foreign exchange operations, proposals for a multiple key-currency system, and suggestions for making currency swap agreements permanent and access to overdrafts automatic. It is argued that, while cooperation is required in any system, the more it is arranged in advance and formalized the less will be the unjustified deflationary bias in policy. Further, a multiple key-currency system is appreciably more unstable than one based on automatic extensions of liquidity, even if both are supported by exchange guarantees. These considerations suggest that adopting a multiple key-currency system, as advocated by F. A. Lutz among others, would be inferior to automatic prearranged currency swaps and overdrafts as suggested by P. B. Kenen and others.
Attachment theory as a working model for the therapist transitioning from early to later recovery substance abuse treatment

Psychotherapy with addicted individuals often involves a shift in therapeutic techniques during the transition from early to later recovery treatment. A conceptual model based in attachment theory can provide a rationale and framework for this shift in the treatment of some addicted persons. Techniques and case material are presented to illustrate how a therapist first “establishes a secure base” and then transitions to “facilitating exploration.” An active, directive, supportive, and educative therapeutic approach that deemphasizes exploratory work in early recovery helps the patient achieve abstinence and develop a sense of security and attachment with the therapist. This sense of therapeutic security combined with ongoing abstinence permits a shift to a more nondirective, expressive therapeutic stance that facilitates the exploratory work of later recovery. Working through these issues in the therapeutic transition provides an excellent opportunity to examine the interpersonal and emotional difficultie...
A low-overhead coherence solution for multiprocessors with private cache memories

This paper presents a cache coherence solution for multiprocessors organized around a single time-shared bus. The solution aims at reducing bus traffic and hence bus wait time. This in turn increases the overall processor utilization. Unlike most traditional high-performance coherence solutions, this solution does not use any global tables. Furthermore, this coherence scheme is modular and easily extensible, requiring no modification of cache modules to add more processors to a system. The performance of this scheme is evaluated by using an approximate analysis method. It is shown that the performance of this scheme is closely tied with the miss ratio and the amount of sharing between processors.
Count Models in Criminology

Crime can be measured in many metrics, including occurrence (yes/no), seriousness (e.g., felony/misdemeanor), and frequency or rate. Frequency can be considered in two analogous ways – how soon until an occurrence (the rate) or how many occurrences per unit such as weeks, months, or years (counts). We can also look at frequency with respect to other units such as population groups or areal units like neighborhoods or counties.
Effects of peroxidized corn oil on performance, AMEn, and abdominal fat pad weight in broiler chicks

Abstract There is a trend to use more alternative lipids in poultry diets, either through animal-vegetable blends, distillers corn oil, or yellow grease. This has resulted in the use of lipids in poultry diets with a higher concentration of unsaturated fatty acids, which have a greater potential for peroxidation. The objective of this experiment was to determine the effects of peroxidized corn oil on broiler performance, dietary AMEn, and abdominal fat pad weight. The same refined corn oil sample was divided into 3 subsamples, 2 of which were exposed to different peroxidative processes. The 3 diets contained the unperoxidized corn oil (UO), a slowly peroxidized corn oil (SO; heated for 72 h at 95°C with compressed air flow rate of 12 L/min), or a rapidly peroxidized corn oil (RO; heated for 12 h at 185°C with compressed air flow rate of 12 L/min). Diets were fed from 0 to 14 d of age with each lipid fed at a 5% inclusion rate, continuing on from 15 to 27 d of age with each lipid fed at a 10% inclusion rate. There were 6 Ross 708 broiler chicks per cage with 10 replicates for each of the 3 dietary treatments. Abdominal fat pad and excreta collection was performed on d 27. Body weight gain, feed intake and feed efficiency were measured for the 0 to 14 and 0 to 27 d periods. The increased level of peroxidation reduced AMEn in broiler diets (UO = 3,490 kcal/kg; SO = 3,402 kcal/kg; RO = 3,344 kcal/kg on an as-is basis; SEM = 12.9, P ≤ 0.01). No significant treatment differences were observed among oil supplemented birds for BW gain, feed intake, feed efficiency, or abdominal fat pad weight. In conclusion, corn oil peroxidation status resulted in a decrease in dietary AMEn, but had minimal effects on broiler performance or fat pad weights.
Structure Formation in Electrorheological Fluids

The structure is fundamental in understanding the physical mechanism and properties of ER fluids. Many experiments indicate that upon application of electric field, dielectric particles in ER fluids first rapidly form chains between the two electrodes which then aggregate into thick columns.1–4 A theoretical prediction5 of a body-centered tetragonal (bct) lattice as the ground state of the thick columns has recently been verified by experiments.1
Examples of deformation-dependent flow simulations of conjunctive use with MF-OWHM

Abstract. The dependency of surface- and groundwater flows and aquifer hydraulic properties on deformation induced by changes in aquifer head is not accounted for in the standard version of MODFLOW. A new USGS integrated hydrologic model, MODFLOW-OWHM, incorporates this dependency by linking subsidence and mesh deformation with changes in aquifer transmissivity and storage coefficient, and with flows that also depend on aquifer characteristics and land-surface geometry. This new deformation-dependent approach is being used for the further development of the integrated Central Valley hydrologic model (CVHM) in California. Preliminary results from this application and from hypothetical test cases of similar systems show that changes in canal flows, stream seepage, and evapotranspiration from groundwater (ETgw) are sensitive to deformation. Deformation feedback has been shown to also have an indirect effect on conjunctive surface- and groundwater use components with increased stream seepage and streamflows influencing surface-water deliveries and return flows. In the Central Valley model, land subsidence may significantly degrade the ability of the major canals to deliver surface water from the Delta to the San Joaquin and Tulare basins. Subsidence can also affect irrigation demand and ETgw, which, along with altered surface-water supplies, causes a feedback response resulting in changed estimates of groundwater pumping for irrigation. This modeling feature also may improve the impact assessment of dewatering-induced land subsidence/uplift (following irrigation pumping or coal-seam gas extraction) on surface receptors, inter-basin transfers, and surface infrastructure integrity.
Human Sexuality Education: Are Special Educators Prepared to Meet the Educational Needs of Disabled Youth?

An apparent critical educational need for students with mild disabilities is comprehensive sex education. However, few data are available to describe the competencies of special educators to deliver sex education to these youth. The purpose of this study was to replicate a previous study examining the extent that preservice and inservice training activities prepared special educators to deliver sex education and their perceived competence levels. The results indicated that special education personnel receive minimal instruction in the delivery of sex education, yet perceive themselves to have average competence for the delivery of the content. Implications for the results are discussed relative to personnel preparation and research issues.
Simulation Analysis of Power Quality Characteristics of Wind Farm and Control Measures

Based on the project of Hexi large-scale wind power connected to grid,the paper established typical wind turbine simulation model,analyzed power quality characteristics such as voltage deviation,voltage fluctuations,and harmonics caused by wind power connected to grid.It simulated effect of using STATCOM to control power quality of wind farm based on analysis of power quality characteristics of wind farm and consideration of compensation effect.The simulation results showed that the STATCOM has good control effect on a variety of power quality problems of wind farm,which can provide a reference for analysis and control of power quality characteristics of wind farm.
Zirconium phosphate nanoparticles as a remarkable solid acid catalyst for selective solvent-free alkylation of phenol

A facile synthesis of α-zirconium phosphate(ZP) nanoparticles as an effective, eco-friendly, and recyclable solid acid catalyst is reported. Polyvinylpyrrolidone(PVP) and polyvinyl alcohol(PVA) were used as organic matrix as dispersing agents and served as a template for the nanoparticles. Hydrogen bonds between ZP and PVA or PVP, along the polymer chains, appear to play an important role for improving the dispersion of in situ formed ZP. Following calcination of PVA/ZP or PVP/ZP, pure hexagonal ZP nanoparticles were obtained. The catalysts were characterized by nitrogen sorption, inductively coupled plasma optical emission spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy(FTIR), scanning electron microscopy, and transmission electron microscopy. Pyridine-FTIR and temperature-programmed desorption of NH3 suggest the presence of Brnsted acid sites. The acidic properties of the catalyst were studied in Friedel-Crafts alkylation of phenol by tert-butanol, producing 2-tert-butylphenol, 4-tert-butylphenol, and 2,4-ditert-butylphenol. The alkylation reaction was performed in the presence of catalysts P2O5/Al2O3, P2O5/SiO2, α-ZrP(prepared in the absence of the polymers), and various ionic liquids. The use of the hexagonal ZP nanoparticle catalyst afforded an excellent phenol conversion(86%) and selectivity towards 4-tert-butylphenol(83%) under optimized reaction conditions. The catalyst was easily recovered from the reaction mixture, regenerated, and reused at least four times without significant loss in the catalytic activity.
Case 52: What Not to Do If You Are a Mentor

You have been appointed as a Departmental Mentor and Facilitator of Resident’s and Fellow’s Research in a medical department. You are excited about the job.
Prolonged cardiac arrest complicating a massive ST-segment elevation myocardial infarction associated with marijuana consumption

Recreational substance use and misuse constitute a major public health issue. The annual rate of recreational drug overdose-related deaths is increasing exponentially, making unintentional overdose as the leading cause of injury-related deaths in the United States. Marijuana is the most widely used recreational illicit drug, with approximately 200 million users worldwide. Although it is generally regarded as having low acute toxicity, heavy marijuana usage has been associated with life-threatening consequences. Marijuana is increasingly becoming legal in the United States for both medical and recreational use. Although the most commonly seen adverse effects resulting from its consumption are typically associated with neurobehavioral and gastrointestinal symptoms, cases of severe toxicity involving the cardiovascular system have been reported. In this report, the authors describe a case of cannabis-associated ST-segment elevation myocardial infarction leading to a prolonged cardiac arrest.
Public Service in "Greeneland"

The essay examines four of Graham Greene's "administrative" novels. The focus of the article is on three basic perspectives that characterize Greene's treatment of bureaucratic systems, in general, and public sector careerists, in particular. The central theme that Greene seems to pursue in all of his novels-the persistent human struggle in the attempt to attain a sense of authentic being-is developed with particular effectiveness when he focuses on career civil servants and their organizational environments. His treatment of the public sector serves to inform us of the ever-present danger of pursuing an inauthentic career in the service of democracy.
Expressivism and the Reliability Challenge

Suppose that there are objective normative facts and our beliefs about such facts are by-and-large true. How did this come to happen? This is the reliability challenge to normative realism. As has been recently noted, the challenge also applies to expressivist “quasi-realism”. I argue that expressivism is useful in the face of this challenge, in a way that has not been yet properly articulated. In dealing with epistemological issues, quasi-realists typically invoke the desire-like nature of normative judgments. However, this is not enough to prevent the reliability challenge from arising, given that quasi-realists also hold that normative judgments are truth-apt beliefs. To defuse this challenge, we need to isolate a deeper sense in which normative thought is not representational. I propose that we rely on the negative functional thesis of expressivism: normative thought does not have the function of tracking normative facts, or any other kind of facts. This thesis supports an argument to the effect that it is misguided to expect an explanation of our access to normative facts akin to the explanations available in regions of thought that have a tracking function. We should be content with explanations of our reliability that take for granted certain connections between our psychology and the normative truths.
Inhibition ofGeotrichum candidum by bicarbonate

In a Sabouraud liquid medium system with NaHCO3 or KHCO3 at as low as 0.1% (w/v), the mycelial growth ofGeotrichum candidum was inhibited by greater than 90%. Bicarbonate may have a potential use as a microbial inhibitor in a variety of food systems.
Outreach Teacher Training Programs: Rural Delivery of Services on Site:

The need of rural areas to provide service to students with visual handicaps and other low incidence conditions is extensively documented. Various service delivery models have been developed for me...
A picture is worth a thousand words: Electronic cigarette content on Instagram and Pinterest.

INTRODUCTION This study examined electronic cigarette (e-cig) content in visual materials posted on the social-media platforms Instagram and Pinterest. Both platforms allow users to upload pictures to the internet and share them globally. Users can search for pictures tagged with specific keywords and phrases. METHODS Using content analysis, this study identified themes in image postings of e-cigs on social media. During five weeks of data collection, keywords were used to identify pictures related to e-cigs. These pictures were then coded into one or more categories. RESULTS The three most popular categories for Instagram posts were marketing, customization and juices/flavors. The three most popular categories for Pinterest posts were customization, marketing and memes. CONCLUSIONS Because of the persuasive power of visuals, it is important to examine communication on Instagram and Pinterest as well as the specific visual messages communicated. Stores and manufacturers use these and similar platforms to communicate with users and potential users; thus it seems that marketers are capitalizing on opportunities for persuasive appeal. The results highlight the popularity of e-cig content on these two social media platforms and reveal an emphasis on marketing and customization.
Asynchronous space-time domain decomposition method with localized uncertainty quantification

Abstract The computational cost associated with uncertainty quantification of engineering problems featuring localized phenomenon can be reduced by confining the random variability of the model parameters within a region of interest. In this case, a localized treatment of mesh and time resolutions is required to capture the effect of the confined material uncertainty on the global response. We present a computational approach for localized uncertainty quantification with the capability of asynchronous treatment of mesh and time resolutions. In particular, we allow each subdomain to have its local uncertainty representation and the corresponding mesh and time resolutions. As a result, computing resources can be directed toward a small region of interest where a model with high spatial and temporal resolutions is required. To verify the numerical implementation, we consider elastic wave propagation in an axially loaded beam. Moreover, we perform convergence studies with respect to the spatial and temporal discretizations as well as the size of an uncertain subdomain. A projectile impacting a composite sandwich plate is considered as an engineering application for the proposed method.
Language of ADHD in Adults on Social Media

Objective: We computationally analyze the language of social media users diagnosed with ADHD to understand what they talk about, and how their language is correlated with users’ characteristics such as personality and temporal orientation. Method: We analyzed approximately 1.3 million tweets written by 1,399 Twitter users with self-reported diagnoses of ADHD, comparing their posts with those used by a control set matched by age, gender, and period of activity. Results: Users with ADHD are found to be less agreeable, more open, to post more often, and to use more negations, hedging, and swear words. Posts are suggestive of themes of emotional dysregulation, self-criticism, substance abuse, and exhaustion. A machine learning model can predict which of these Twitter users has ADHD with an out-of-sample AUC of .836. Conclusion: Based on this emerging technology, conjectures of future uses of social media by researchers and clinicians to better understand the naturalistic manifestations and sequelae of ADHD.
Unipolar magnetoelectric magnetic tunnel junction devices and circuits

Many magneto-electric (ME) and similar advanced devices with inherent memory require switching between a positive and negative voltage (or current) to switch between the ME spin states. Use of such a “bipolar” switching regime requires additional components as well as complex circuitry. We here introduce variants to the magneto-electric magnetic tunnel junction device (ME-MTJ) which permits direct driving of ME-MTJ devices by a prior ME-MTJ device. This is the unipolar magneto-electric magnetic tunnel junction (UMMTJ) device. It enables full logic circuitry to be implemented without level shifting between each logic element.
Genetic Population Structure and Accuracy of Morphological Assessment in Alosa aestivalis (Blueback Herring) and A. pseudoharengus (Alewife)

Abstract Alosa aestivalis and A. pseudoharengus are herring congeners that are important forage species for piscivorous fish and birds. We measured population structure metrics for these species using microsatellite markers. The Southern Gulf of Maine study area allowed the assessment of these species at an inter- and intra-watershed level. We found no detectable population structure within or among watershed for either species which agrees with other recent research. Our results support regional-scale (e.g., Gulf of Maine) plans for management for A. aestivalis and A. pseudoharengus. We found that 5.4% of our samples were hybrids. Our study adds to a growing body of evidence that hybridization and introgression should be management concerns for these species, and precautions should be taken to preserve species barriers. An error rate of morphological identification was calculated by comparing morphological identifications against genetic classifications. We found an overall identification error rate of 1...
Moral Drivers of Interpersonal Relationships at Work

The purpose of this symposium is to examine how issues related to individual values, morality, and ethics influence the way in which people build relationships with leaders and co-workers, and ulti...
Gene Polymorphisms for PAI-1 Are Associated with the Angiographic Extent of Coronary Artery Disease.

Localized regulation of fibrinolytic protein gene expression is associated with the histologic extent of atherosclerosis. This regulation may be dependent on the presence of certain fibrinolytic protein gene polymorphisms. The relationship between the plasminogen activator inhibitor (PAI)-1 HindIII and the tissue plasminogen activator (t-PA) EcoR1 gene polymorphisms and the extent of coronary artery disease (CAD) were investigated in 49 Caucasian patients with symptomatic CAD. There was a strong association between PAI-1, but not t-PA, gene polymorphisms and the extent of CAD detected by coronary angiography. Patients homozygous for the presence or absence of the PAI-1 HindIII (1/1, 2/2 PAI-1) gene polymorphisms had a significantly greater extent of CAD (number of diseased vessels) than patients with the respective heterozygous forms (vs. 1/2 PAI-1, P = 0.05). Stepwise ordinal multiple regression analysis of classic CAD risk factors and fibrinolytic protein genotypes indicated that only the PAI-1 genotypes were predictive of the extent of angiographic CAD (P = 0.019). Analysis of variance between classic risk factors and fibrinolytic protein genotypes identified an association between t-PA genotypes and a history of prior infarction or stroke. Fibrinolytic gene polymorphisms for PAI-1 are associated with the extent of CAD in symptomatic patients and with certain risk factors for coronary atherosclerosis.
Interaction of Insulin-Like Growth Factor-Binding Protein 3 With Hyaluronan and Its Regulation by Humanin and CD44.

Insulin-like growth factor-binding protein-3 (IGFBP-3) belongs to a family of IGF-binding proteins. Humanin is a peptide known to bind residues 215–232 of mature IGFBP-3 in the C-terminal region of...
Boarding the Transhumanist Train: How Far Should the Christian Ride?

The transhumanist (H+) train has pulled out of the station and is now racing toward its destination: technoutopia. Via GNR--Genetics, Nanotechnology, and Robotics--the H+ engineer is guiding us toward posthumanity where our descendents will enjoy superintelligence in digital, disembodied, and immortal form. How far will the Christian want to ride this train? I recommend that the Christian board the H+ train and ride the rails of technological progress as far as improved medical therapies, increased longevity, advanced robotics, and other enhancements in human well-being and flourishing. But, I further recommend disembarking before the unrealistic and even undesirable posthuman utopia which would amount to the end of the line for humanity.
Reovirus: Friend and Foe.

Mammalian orthoreovirus (reovirus) is a powerful tool for studying viral replication and pathogenesis. Most reovirus infections are subclinical; however, recent work has catapulted reovirus into the clinical spotlight. Owing to its capacity to kill cancer cells more efficiently than normal cells, reovirus is under development as a therapeutic for a variety of cancers. New efforts have focused on genetically engineering of reovirus to increase its oncolytic capacity, and determining how reovirus potentiates immunotherapy. Other recent studies highlight a potential role for reovirus in celiac disease (CeD). Using mouse models of CeD, reovirus caused loss of oral tolerance to dietary antigens, opening the possibility that reovirus could trigger CeD in humans. We will focus on new developments in reovirus oncolysis and studies suggesting a role for reovirus as a trigger for celiac disease (CeD) that makes reovirus a potential friend and foe to human health.
An integrated flow reactor-membrane filtration system for heterogeneous photocatalysis. Part II: Experiments on the ultrafiltration unit and combined operation

A batch-recirculated photoreactor was combined with a hollow-fibre membrane ultrafiltration (UF) unit for heterogeneous photocatalysis applications. This paper focuses on the operation and modelling of the UF process component and on the performance of the integrated photoreactor–UF process assembly. Methylene blue and titanium dioxide (Degussa P-25) were used as the test pollutant and photocatalyst, respectively. The influence of cross-flow velocity, transmembrane pressure, and TiO2 dose on the permeate flux through the hollow fibre membrane is described. These data are modelled on the basis of concentration polarization and gel layer formation at the membrane surface/feed slurry boundary. The operation of the integrated photoreactor–UF assembly over ten repeat cycles is described. Photocatalyst separation was complete as gauged by nephelometric turbidity measurements. However, a systematic degradation in the photocatalyst performance was noted with each repeat cycle. Dynamic laser light scattering data are consistent with agglomeration of the TiO2 particles as a result of the UF process, and suggest a possible factor in the degraded photocatalytic activity. Possible solutions to this problem are finally presented.
Characterization of the FeV=O Complex in the Pathway of Water Oxidation

Hypervalent FeV =O species are implicated in a multitude of oxidative reactions of organic substrates, as well as in catalytic water oxidation, a reaction crucial for artificial photosynthesis. Spectroscopically characterized FeV species are exceedingly rare and, so far, were produced by the oxidation of Fe complexes with peroxy acids or H2 O2 : reactions that entail breaking of the O-O bond to form a FeV =O fragment. The key FeV =O species proposed to initiate the O-O bond formation in water oxidation reactions remained undetected, presumably due to their high reactivity. Here, we achieved freeze quench trapping of six coordinated [FeV =O,(OH)(Pytacn)]2+ (Pytacn=1-(2'-pyridylmethyl)-4,7-dimethyl-1,4,7-triazacyclononane) (2) generated during catalytic water oxidation. X-ray absorption spectroscopy (XAS) confirmed the FeV oxidation state and the presence of a FeV =O bond at ≈1.60 A. Combined EPR and DFT methods indicate that 2 contains a S=3/2 FeV center. 2 is the first spectroscopically characterized high spin oxo-FeV complex and constitutes a paradigmatic example of the FeV =O(OH) species proposed to be responsible for catalytic water oxidation reactions.
Mutant and Recombinant Phages Selected from In Vitro Coevolution Conditions Overcome Phage-Resistant Listeria monocytogenes

ABSTRACT Bacteriophages (phages) are currently available for use by the food industry to control the foodborne pathogen Listeria monocytogenes. Although phage biocontrols are effective under specific conditions, their use can select for phage-resistant bacteria that repopulate phage-treated environments. Here, we performed short-term coevolution experiments to investigate the impact of single phages and a two-phage cocktail on the regrowth of phage-resistant L. monocytogenes and the adaptation of the phages to overcome this resistance. We used whole-genome sequencing to identify mutations in the target host that confer phage resistance and in the phages that alter host range. We found that infections with Listeria phages LP-048, LP-125, or a combination of both select for different populations of phage-resistant L. monocytogenes bacteria with different regrowth times. Phages isolated from the end of the coevolution experiments were found to have gained the ability to infect phage-resistant mutants of L. monocytogenes and L. monocytogenes strains previously found to be broadly resistant to phage infection. Phages isolated from coinfected cultures were identified as recombinants of LP-048 and LP-125. Interestingly, recombination events occurred twice independently in a locus encoding two proteins putatively involved in DNA binding. We show that short-term coevolution of phages and their hosts can be utilized to obtain mutant and recombinant phages with adapted host ranges. These laboratory-evolved phages may be useful for limiting the emergence of phage resistance and for targeting strains that show general resistance to wild-type (WT) phages. IMPORTANCEListeria monocytogenes is a life-threatening bacterial foodborne pathogen that can persist in food processing facilities for years. Phages can be used to control L. monocytogenes in food production, but phage-resistant bacterial subpopulations can regrow in phage-treated environments. Coevolution experiments were conducted on a Listeria phage-host system to provide insight into the genetic variation that emerges in both the phage and bacterial host under reciprocal selective pressure. As expected, mutations were identified in both phage and host, but additionally, recombination events were shown to have repeatedly occurred between closely related phages that coinfected L. monocytogenes. This study demonstrates that in vitro evolution of phages can be utilized to expand the host range and improve the long-term efficacy of phage-based control of L. monocytogenes. This approach may also be applied to other phage-host systems for applications in biocontrol, detection, and phage therapy.
Executive and Social Functioning Across Development in Children and Adolescents With Prenatal Alcohol Exposure.

Background Prenatal alcohol exposure (PAE) is linked to a variety of neurodevelopmental challenges, including social functioning (SF) and executive functioning (EF) deficits. These deficits present differently across developmental stages from preschool age to adolescence. Methods The post hoc analyses described here were conducted on data from 83 preschool-age children with PAE (early childhood group; ages 2.5 to 5.0) and 95 adolescents (49 with PAE, 46 controls; ages 8 to 16). Each child completed EF tasks as part of several prior studies. Parents completed social and communication inventories about their child's abilities. Thirty-three participants from the early childhood group returned for a 4-year follow-up and completed both SF and EF measures. Results Both the early childhood and adolescent groups with PAE showed deficits in SF and EF. There was a relationship between SF and EF within the adolescent PAE group that was not present in the adolescent control group or the early childhood PAE group. However, at the 4-year follow-up (Mage = 8.45), participants originally in the early childhood PAE group also demonstrated this relationship. Conclusions These findings support previous research on EF/SF deficits in adolescents with PAE while also addressing a gap in the literature concerning early childhood research on this topic. Additionally, these findings suggest that the relationship between EF and SF deficits may strengthen throughout development. This line of research highlights potential sensitive periods for SF and EF training in children with PAE and suggests that fetal alcohol spectrum disorders programs consider targeting EF training as a component of social skill interventions.
The Free-Market Family : Liberalism, Families, and Government’s Responsibility to Regulate the Market

Despite the harmful effects of contemporary market forces on families, government’s responsibility to regulate the market to support families remains significantly undertheorized in liberal theory. This chapter begins to fill that gap. The first part argues that a fundamental responsibility of the liberal state must be to regulate societal institutions in order to support the circumstances that families need to thrive. The second part outlines the many ways that market forces in the United States are undermining the health of families. The third part argues that, insofar as the market isn’t delivering families the circumstances they need, it is government’s role to regulate markets and to structure the larger economy in order to support families. This part also lays out the tenets that should govern economic regulation to support families. These tenets would embed both markets and families into a larger economy whose purpose is to ensure that individuals get the resources they need – through families, market, and the government – to thrive.
Long-term survival after descending thoracic and thoracoabdominal aortic aneurysm repair.

Abstract Objective Patients with descending thoracic aortic aneurysms (dTAA) or thoracoabdominal aortic aneurysms (TAAA) often have a variety of medical comorbidities. Those that are deemed acceptable for intervention undergo complicated repairs with good early outcomes. The purpose of this study was to identify variables that were associated with mortality over time. Methods This was a retrospective review of a prospectively maintained database at our institution from 1983 to 2015. Patients were included if they underwent open or endovascular repair for dTAA or TAAA. Patients were excluded if they were intervened on for traumatic transections. The primary outcome for the study was long-term survival. Secondary outcomes included aortic-related mortality. We had mortality and survival data on all patients. Results A total of 946 patients met our study criteria with a median follow-up of 102.8 months (interquartile range [IQR], 58.9-148.2 months). The median age of the cohort was 71 years (IQR, 63-77 years) with the majority of patients being male (58.1%). The extent of TAAA pathology was as follows: type I (14.2%), type II (21.2%), type III (17.1%), type IV (26.2%), and dTAA (21.2%). A total of 147 patients (15.5%) had a prior dissection. The median diameter of aneurysm was 6.4 cm (IQR, 6.0-7.0 cm). A total of 158 patients (16.7%) underwent endovascular repair over the study period. Variables associated with mortality over time were age, surgical era, acute pathology, dissection, preoperative creatinine, and type IV TAAAs. In addition, experiencing the following complications in the postoperative period was associated with mortality over time: neurological, cardiac, and pulmonary. Aortic-related mortality was 2.1% (n = 20) over the study period. Patients who underwent endovascular repair for acute conditions had better long-term survival when compared with open repair. However, there were no differences in long-term survival between open and endovascular repair for nonacute cases. In addition, repair in the more modern era was associated with improved survival. Conclusions TAAAs can be repaired with reasonable perioperative mortality rates. Once patients undergo repair of their aneurysm, aortic-related mortality remains low. The addition of endovascular options has dramatically changed management of patients with dTAA and TAAA. Further, endovascular repair was associated with decreased perioperative mortality and significantly increased long-term survival in acute patients. Patients undergoing TAAA repair are generally considered high risk and therefore require extensive long-term follow-up for management of their comorbidities and complications, because these are the main contributors to mortality over time.
A systematic review of sutureless vascular anastomosis technologies

ABSTRACT Vascular anastomoses typically involve a handsewn technique requiring significant surgical training, expertise, and time. The aim of our systematic review was to identify and describe sutureless vascular anastomosis techniques. We performed a systematic review of all sutureless vascular anastomosis technologies published in MEDLINE, PubMed, Embase, CINAHL, Cochrane, Web of Science, and Scopus Library databases and a patent review using US Patent and Trade Office Application, US Patent and Trademark Office Patent, Google Patents, Lens, Patent Quality Through Artificial Intelligence, SureChEMBL, and E-Space Net. Data from inclusion studies and patents published between January 1, 1980 and July 15, 2021 were abstracted to describe their category, anastomosis type and configuration, study types, and advantages and disadvantages encountered with each technology. Two hundred eleven original studies and 475 patents describing sutureless vascular anastomosis technologies were identified. In the literature, stents/stent–grafts/grafts (n = 61), lasers (n = 53), and couplers (n = 27) were the predominant device categories. In the patent review, adhesive technologies (n = 103), stents/stent–grafts/grafts (n = 68), and mechanical connectors (n = 61) predominated. The majority of studies involved in vivo animal studies (n = 193); 32.2% (n = 68) of investigations involved human trials; and 17.9% (n = 85) of patent technologies were approved by the US Food and Drug Administration. The main advantages described for sutureless anastomosis technologies included faster procedure time and greater patency rates compared with handsewn anastomoses. The main disadvantages included reduced vessel compliance, stenosis, leakage, and device costs. The appeal of sutureless technology is substantiated by numerous animal trials, but their use in humans remains limited. This may be a reflection of strict regulatory criteria and/or vascular complications associated with currently available technologies.
Hydroburst Test Methodology for Evaluation of Composite Structures

The University of Texas at Austin Center for Electromechanics (UT-CEM) is under contract to develop high-speed composite flywheel systems for a number of energy and power averaging applications in the transportation and space industries. Safe and reliable design of composite flywheels requires well-characterized material properties. Efforts have been focused on design optimization of a hydroburst test technique that demonstrates a useful method for characterizing the hoop (circumferential) properties of filament wound composite rings, and those results are then available to predict the performance of full-scale flywheels. To demonstrate the usefulness of this method, this paper discusses typical hydroburst techniques to determine material properties for filament-wound composite rings. Test results are also presented. Seal design is a key element of this fixture, and a design has evolved that provides nearly uniform radial loading on the inside surface of the composite ring, while minimizing axial bending. Correlations of tow strand data with hydroburst lamina data are presented. Also discussed are hydroburst test applications for flaw assessment and fatigue property evaluation.
Extracting and Characterizing Hydra RNA: Modifications to Allow Extraction of Undegraded Material in the Presence of High Levels of Degradative Enzymes

Because hydra is rich in degradative enzymes (i e., nucleases and proteases), the application of conventional techniques for the isolation of macromolecules invariably results in the isolation of degraded material (Voland, 1975). We describe herein a modified phenol—chloroform procedure for the extraction of total RNA from Hydra oligactis which overcomes these difficulties.
Discriminating Nature of the Aspartyl-tRNA Synthetases from Three Human Pathogens

Protein synthesis is essential for all known life. Accurate translation during protein synthesis is primarily achieved by pairing an amino acid with its cognate tRNA. For the amino acid asparagine,...
The potential role of vitamin D in the progression of benign and malignant melanocytic neoplasms.

Hormonally active vitamin D 3 , 1,25-(OH) 2 D 3 , is believed to have a role in the prevention of cancer formation and in limiting the aggressiveness of cancers that do arise. Therefore, much interest is presently being focused on 1,25-(OH) 2 D 3 and its analogues as potential treatments for various cancers including melanoma. This article discusses the evidence in favour of a role for 1,25-(OH) 2 D 3 in protection against the progression of melanocytic lesions and also summarizes the mechanisms by which 1,25-(OH) 2 D 3 may act to protect against melanoma development and progression.
Tumor Cell-selective Cytotoxicity of Matrix Metalloproteinase-activated Anthrax Toxin

Matrix metalloproteinases (MMPs) are overexpressed in a variety of tumor tissues and cell lines, and their expression is highly correlated to tumor invasion and metastasis. To exploit these characteristics in the design of tumor cell-selective cytotoxins, we constructed two mutated anthrax toxin protective antigen (PA) proteins in which the furin protease cleavage site is replaced by sequences selectively cleaved by MMPs. These MMP-targeted PA proteins were activated rapidly and selectively on the surface of MMP-overexpressing tumor cells. The activated PA proteins caused internalization of a recombinant cytotoxin, FP59, consisting of anthrax toxin lethal factor residues 1-254 fused to the ADP-ribosylation domain of Pseudomonas exotoxin A. The toxicity of the mutated PA proteins for MMP-overexpressing cells was blocked by hydroxamate inhibitors of MMPs, including BB94, and by a tissue inhibitor of matrix metalloproteinases (TIMP-2). The mutated PA proteins killed MMP-overexpressing tumor cells while sparing nontumorigenic normal cells when these were grown together in a coculture model, indicating that PA activation occurred on the tumor cell surface and not in the supernatant. This method of achieving cell-type specificity is conceptually distinct from, and potentially synergistic with, the more common strategy of retargeting a protein toxin by fusion to a growth factor, cytokine, or antibody.
Rivaroxaban-induced epidural hematoma and cauda equina syndrome after total knee arthroplasty: a case report.

Deep venous thrombosis and pulmonary embolism are a major cause of morbidity and mortality after arthroplasty. Chemoprophylaxis has been effective in reducing the incidence of symptomatic deep venous thrombosis, but not of pulmonary embolism, after total hip arthroplasty (THA) and total knee arthroplasty (TKA)1. Rivaroxaban, an orally administered factor Xa inhibitor, has recently been introduced for reducing the incidence of deep vein thrombosis (DVT) and pulmonary embolism (PE) after hip and knee arthroplasty2, 3, 4, 5. Factor Xa catalyzes the conversion of prothrombin to thrombin. Because a single molecule of Xa can generate thousands of thrombin molecules, factor Xa is an excellent drug target6. In many ways, rivaroxaban is an ideal drug, being orally administered, usually requiring no monitoring and having a faster onset of action than warfarin. However, like other effective thromboprophylactic agents, rivaroxaban has been associated with an increased incidence of bleeding from surgical wounds or other sites. Here we present a case of a patient who developed a symptomatic epidural hematoma after postoperative administration of rivaroxaban. The patient was informed that we wanted to submit this case for publication and gave written permission. The purpose of this report was to describe an unanticipated, early complication of a new type of DVT chemoprophylaxis after total knee arthroplasty, thus educating patients and surgeons about the possible risks of chemoprophylaxis. This patient appeared to develop the complication while bridging from a different anticoagulant to rivaroxaban. We now recommend extreme caution re‐using rivaroxaban in the setting of recent spinal anesthesia after arthroplasty.