cssi/SciSciGPT-SciSciNet huggingface dataset:
Stability of a mixture of technetium Tc 99m sulfur colloid and lidocaine hydrochloride.

Purpose. The stability of a mixture of technetium Tc 99m sulfur colloid and lidocaine hydrochloride for up to eight hours was studied. Methods. Three vials of technetium Tc 99m sulfur colloid were compounded according to the manufacturer’s instructions. From each of the three vials, five samples were withdrawn into syringes with needles: 0.4, 0.45, 0.5, 0.63, and 1 mCi for testing after storage for zero, one, two, four, and eight hours, respectively. Each syringe contained the customary patient dose of 0.4 mCi at the time of testing. Fresh 0.9% sodium chloride injection was used to bring the volume of each syringe to 0.2 mL, and 0.2 mL of lidocaine hydrochloride 1% was added to bring the final volume to 0.4 mL. Measurements of pH, radiochemical purity, and particle size were conducted after the indicated storage times for each group of samples. Results. The pH of samples showed no substantial change over the eight-hour storage period, with individual values in the range of 4.5–5.5. Radiochemical purity did not change substantially, with values ranging from 98.9% to 99.9%. There was no meaningful change in the amount of radioactivity retained by filtration and no increase in particle size. Conclusion. Adding lidocaine hydrochloride 1% to syringes containing technetium Tc 99m sulfur colloid and storing the syringes for up to eight hours had no effect on the pH or radiochemical purity of the mixture or on the radioactivity retained by a filter.
Clinical outcome of overlapping stents in hemodialysis access

PURPOSE Endovascular stents have recently been shown to extend access patency in thrombosed and stenotic arteriovenous grafts. Given this improved patency, stent placement has outpaced balloon angioplasty in hemodialysis (HD) access interventions. However, concern remains over localized corrosion and increased neointimal hyperplasia of overlapping stents in the access circuit and whether this promotes premature stent failure. METHODS This is a retrospective analysis of HD patients referred for access dysfunction during a 2-yr period. Using a prospectively collected, vascular access database, we identified 76 patients seen for follow-up angiography due to access dysfunction after stent placement. We compared the outcomes of overlapping vs. non-overlapping stents in measured primary assisted patency and mean percent luminal diameter as a marker of lesion severity. RESULTS The two groups did not differ significantly in demographics or comorbid conditions. Only gender had a significant discrepancy between the two groups, with 65.5% vs.42.9% male (p=0.01) in the overlapping vs. non-overlapping stent groups, respectively. The mean percent luminal stenosis was found to be 83.7 ± 17.3 and 85.5 ± 12.6 (p=0.55) for the overlapping vs. non-overlapping stent groups, respectively. For overlapping and non-overlapping stents, 30-day primary patency was 94% and 89%, respectively, 60-day primary patency was 77% and 63%, respectively, and 90-day primary patency was 68% and 50%, respectively. Using multiple regression analysis, no risk factors were identified to be associated with the severity of luminal stenosis. No identifiable risk factors were found to be associated with improved primary patency. In particular, overlapping vs. non-overlapping stents were not identified as a statistically significant factor influencing primary (assisted) patency (hazards ratio 0.60; 95% CI 0.34 to 1.06; p>0.05). CONCLUSIONS This study provides evidence that the theoretical concern of metal on metal corrosion and increased neointimal hyperplasia that can be seen with overlapping stents does not play a significant clinical role.
Abstract B60: Interleukin-1 expression and inflammasome activation in human malignant glioma

Introduction: Interleukin 1 (IL-1) is a major proinflammatory cytokine produced by myeloid cells and is implicated in neurodegeneration. In human brain, IL-1 is expressed by microglia and contributes to neuroinflammation and neuronal toxicity. IL-1 is also the major activator of human astrocyte and glioma cells. In this study, we investigated the mechanism of IL-1 α and IL-1β expression and activation in human glioma cells. Methods: Human glioblastoma cell lines (U87, U251, and SN19) as well as patient-derived glioma cell lines were stimulated with cytokines (IL-1/IFNγ) or TLR ligands (LPS or poly IC) and the expression of IL-1α and IL-1β was studied by real-time PCR, western blot analysis, and ELISA. The glioma secretome was investigated using quantitative mass spectrometry. Results: We found that while primary human astrocytes show IL-1 translational block (no protein made despite high level of mRNA), but GBM cells responded strongly to IL-1 itself to produce large amounts of IL-1α and IL-1β mRNA and proteins. Furthermore, GBM cells processed pro-IL-1β (31kDa) to mature IL-1β (17kDa) by activating the NALP3 inflammasome. Both ATP and nigericin induced IL-1β processing by upregulating NALP3. Proteomics analysis of IL-1 stimulated GBM cell secretome revealed several highly upregulated proteins (IL-8, MCP-1, tenascin-C, pentraxin 3, galectin 1, and MMP2), all of which are implicated in glioma progression. Furthermore, many extracellular matrix proteins (ECM) and ECM-related proteins were downregulated by IL-1 in the glioma secretome. Discussion: We propose that the ability of malignant glioma cells to produce IL-1 protein can set off chronic smoldering inflammation and induce epithelial-mesenchymal transition (EMT) resulting in increased migratory capacity, a unique gene signature, and increased immune signaling (NF-κB, pStat3). There is potential for IL-1 to be a biomarker/surrogate marker for a subset of GBM and that IL-1 blockade could benefit GBM patients. Citation Format: Leonid Tarassishin, Jihyeon Lim, Sunhee C. Lee. Interleukin-1 expression and inflammasome activation in human malignant glioma. [abstract]. In: Proceedings of the Third AACR International Conference on Frontiers in Basic Cancer Research; Sep 18-22, 2013; National Harbor, MD. Philadelphia (PA): AACR; Cancer Res 2013;73(19 Suppl):Abstract nr B60.
The Distribution of Stars near the Supermassive Black Hole in the Galactic Center

We analyze three sets of infrared star counts in the inner ~0.5 pc of the Galactic center. We perform statistical tests on the star counts and model in detail the extinction field and the effects of dwarf-giant collisions on the luminosity function. We find that both the star counts and the depletion of the brightest stars in the inner ~0.05 pc can be explained by an n ∝ r-α stellar cusp with α in the range 3/2-7/4, in which the envelopes of the brightest giants are destroyed by stellar collisions. Such a cusp is consistent with the Bahcall-Wolf solution for the distribution of stars that have undergone two-body relaxation around a black hole. We show that systematic uncertainties due to variable extinction and unrelaxed stars are probably small, but deeper star counts are required to confirm these results. We estimate that the tidal disruption rate of cusp stars by the black hole is a few × 10-5 yr-1.
Comparative phylogeography and population genetic structure of three widespread mollusc species in the Mediterranean and near Atlantic

Although several studies have evaluated the genetic structure and phylogeographic patterns in many species of marine invertebrates, a general model that applies to all of them remains elusive. For example, some species present an admixture of populations with high gene flow, whereas others exhibit more complex patterns characterized by small-scale unstructured genetic heterogeneity, even at a local scale. These differences are thought to be due to clear biological aspects such as direct versus indirect development, or the presence of lecithotrophic versus planktotrophic larvae, but few studies compare animals with similar distributions and life modes. Here, we explore the phylogeographic and genetic structure patterns in two chiton (Chiton olivaceus and Lepidopleurus cajetanus) and one abalone (Haliotis tuberculata) species co-occurring in the same habitat. Samples were obtained from shallow rocky bottoms along the Iberian Peninsula (Atlantic and Mediterranean coasts), Italy, Croatia and Greece, and the mitochondrial markers COI and 16S rRNA gene were sequenced. Our data show evidence of admixture and population expansion in C. olivaceus and H. tuberculata, whereas L. cajetanus exhibited a ‘chaotic patchiness’ pattern defined by a high genetic variability with locality-exclusive haplotypes, high genetic divergence, and a lack of geographic structure. Shared haplotypes were sampled in both coasts of Iberia (for H. tuberculata) and in the Western and Eastern Mediterranean (for C. olivaceus), potentially indicating high dispersal ability and a recent expansion. The processes underlying the fine-scale structuring in L. cajetanus remain a mystery. These results are especially interesting because the reproductive mode of the two chitons is similar but differs from that of the abalone, with a veliger larva, while instead the genetic structure of C. olivaceus and H. tuberculata are similar, thus contrasting with predictions based on the life history of the three molluscs and showing that the genetic patterns of marine species may be shaped by many factors, including historical ones.
The test performance of 16 to 21-month-olds in home and laboratory settings

Young children were tested with the Bayley Mental Scale in a familiar home setting and unfamiliar research room. Thirty-six children, 18 boys and 18 girls, between 16 and 21 months were tested both at home and in the research laboratory, in two groups having different orders. Those children tested first in the laboratory and then in the home had a statistically significant increase of 9.8 points in the mean Mental Development Index; there was no increase for those tested first in the home and then the laboratory. Examination of item groupings suggested that the effect was largely upon verbal performance.
THREE‐DIMENSIONAL CRACK‐SHAPE EFFECTS DURING THE GROWTH OF SMALL SURFACE FATIGUE CRACKS IN A TITANIUM‐BASE ALLOY

— A basic study was performed on the evolution of three-dimensional shapes of small surface fatigue cracks during fatigue, and the effect of this evolution on small-crack growth behavior of a titanium-base alloy. Specifically, the nature and the magnitude of variations in crack aspect ratio, a/c (a is the crack depth and c is the half-surface crack length), during cyclic crack growth and its impact on growth rates have been studied. Experiments were performed on naturally initiated micro-cracks in a microstructure consisting of equiaxed primary-α2 phase in a Widmanstatten (transformed β) matrix. Several cracks under stress ratio (R) levels of 0.1 and −1, were studied. A specialized experimental system, consisting of a laser interferometer (to measure precisely the small-crack surface displacements), and a photo microscope (to automatically and continuously photograph the fatigue micro-cracks) was employed in the study. Apparent aspect ratios of surface cracks were calculated from the compliance response and the surface crack length data as a function of fatigue cycles. These data enabled accurate calculations of growth rates at the surface crack tip as well as the tip at depth in the bulk over the entire crack growth period, thus giving an insight into the crack growth process. Measurements of closure levels of small cracks were also performed and were used to partly account for the differences in growth rates. In the comparisons of small-crack growth data with the large-crack data, surface growth rates correlated relatively well with the large-crack data. Growth rates at depth exhibited large variations due to the irregularity of crack fronts at this location, and these rates deviated significantly from the large-crack behavior. Additionally, these growth rates varied between different cracks. An attempt was made to rationalize these observations in terms of the effects of inhomogeneities present in the microstructure.
Mutant Huntingtin in Glial Cells Exacerbates Neurological Symptoms of Huntington Disease Mice

Huntington disease (HD) is caused by an expansion of the polyglutamine (polyQ) repeat (>37Q) in huntingtin (htt), and age of onset is inversely correlated with the length of the polyQ repeat. Mutant htt with expanded polyQ is ubiquitously expressed in various types of cells, including glia, but causes selective neurodegeneration. Our recent study demonstrated that expression of the N-terminal mutant htt with a large polyQ repeat (160Q) in astrocytes is sufficient to induce neurological symptoms in mice (Bradford, J., Shin, J. Y., Roberts, M., Wang, C. E., Li, X.-J., and Li, S. H. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 22480-22485). Because glia-neuron interactions are critical for maintaining the normal function and survival of neurons in the brain and because mutant htt is more abundant in neurons than in glial cells, it is important to investigate whether glial htt can still contribute to HD pathology when mutant htt is abundantly expressed in neuronal cells. We generated transgenic mice that express mutant htt with 98Q in astrocytes. Unlike our recently generated htt-160Q transgenic mice, htt-98Q mice do not show obvious neurological phenotypes, suggesting that the length of the polyQ repeat determines the severity of glial dysfunction. However, htt-98Q mice show increased susceptibility to glutamate-induced seizure. Mice expressing mutant htt in astrocytes were mated with N171-82Q mice that express mutant htt primarily in neuronal cells. Double transgenic mice expressing mutant htt in both neuronal and glial cells display more severe neurological symptoms and earlier death than N171-82Q mice. These findings indicate a role of glial mutant htt in exacerbating HD neuropathology and underscore the importance of improving glial function in treating HD.
The isolation and amino acid composition of the α chains of chicken-bone collagen

Abstract 1. 1.|A fraction of undegraded chicken-bone gelatin has been obtained by gel filtration which contained only the α components. 2. 2.|Three distinct α components, corresponding to the α1, α2, and α3 chains were isolated by free flow electrophoresis. 3. 3.|Higher molecular weight components, similar to the β and γ fractions of soft-tissue collagens were also observed. 4. 4.|The data indicates that the structure of the macromolecule of bone collagen is similar to the collagen macromolecules of unmineralized connective tissues.
Urban development and death: Bombay City, 1870-1914.

Historians, statesmen, administrators, nationalists and others have disagreed sharply about the impact of modernization in the era of Western domination. Did Western rule provide the tools for Indian progress but did economically medieval, ‘other-worldly’ Indians fail to maximize the benefits of modernization and even thwart advances? Conversely, did Western imperialism systematically impoverish India by making it a ‘satellite,’ freezing the subcontinent into a neo-feudal social pattern while sucking up its wealth? Finally, is a ‘new revisionist’ interpretation correct that India experienced real if undramatic economic growth during the Western era and that notions of exploitation or Indian suffering induced by development were myths? Interpretations expressing either the great success and benign innovations of Western rule, or its exploitiveness both appear flawed, according to Bombay's modernizing experience. Bombay underwent a great expansion of wealth and became the source of India's new factory textile production, the hub of a great newwork of trasport and trade, and the cosmopolitan abode of wealth Indian merchants, industrialist and professionals, whose affluence, modernity, industrializing activies and eventual nationalist orientation distinguished them from a supine or neo-feudal comprador class, cooperating with Western masters in exploiting ‘natives’ for a myrmidon's share of the profits. Alternatively, Bombay's prosperity did not flow down to the masses; its modernization was complex, dynamically helping to produce progress and wealth, but for some decades impoverishing and destroying many lives. In the half-century of rapid development preceding the first world war, the great majority of Bombay's populace, its ordinary working classes, experienced significant declines in living standards, worsening environmental conditions and escalating death-rates. Diminished real income and increased mortality among Bombay's ordinary inhabitants warn against extrapolating from rising indices of material production an optimistic conclusion about the general human condition in the city or in British India.
Formation of free fatty acid and ceramide during brain handling: lability of sphingomyelin.

: Intact brain and brain homogenates readily form free fatty acids and ceramides, even in the cold during subcellular isolation procedures. The fatty acid formation is slightly stimulated by chelators and might be due to phospholipid hydrolysis by lysosomal phospholipases. The ceramide formation is accompanied by loss of sphingomyelin and is apparently due to the action of neutral, metal ion-activated sphingomyelinase. The latter reaction is inhibited by EDTA whereas both degradative processes are inhibited by mercuriphenylsulfonate, the thiol-reacting inhibitor. Cerebroside does not seem to be a source of accumulated ceramide.
Effect of organic solvents on in vitro human skin water barrier function

Skin barrier disruption caused by organic solvents to human cadaver dermatomed skin was evaluated using an in vitro model system. Resultant changes in transepidermal water loss (TEWL), as measured with an evaporimeter, were recorded after topical application of either acetone, chloroform:methanol 2:1, hexane, hexane:methanol 2:3, or the control, water, for exposure times of 1, 3, 6, and 12 min. The resultant lipid/solvent mixture was removed and analyzed for its lipid content. The ability of the different solvents to induce changes in the skin's barrier function was assessed by comparing pre- to post-solvent exposure TEWL (delta TEWL). When compared to the controls, water and unexposed skin, chloroform:methanol 2:1 caused the greatest significant increase in TEWL, followed by hexane:methanol 2:3. Acetone and hexane showed no difference in TEWL from the controls. Besides solvent, exposure time was a significant independent variable for predicting delta TEWL, and the interaction of the two (exposure time and solvent type together) was the strongest predictor. Lipid analysis of the extracts revealed that all the solvents removed comparable quantities of the surface lipids (triglycerides, wax esters, squalene, cholesterol esters). Stratum lipids--ceramides, free fatty acids, and cholesterol--extracted by chloroform:methanol 2:1 and hexane:methanol 2:3 were comparable and significantly greater than those extracted by acetone and hexane. These two solvents failed, however, to induce comparable changes in TEWL, as chloroform:methanol 2:1 induced a significantly greater delta TEWL than hexane:methanol 2:3. Additionally, no individual lipid class extracted by either chloroform:methanol 2:1 or hexane:methanol 2:3 proved to be a significant or accurate variable for predicting delta TEWL. This suggests that the mechanism by which topical chloroform:methanol 2:1 and hexane:methanol 2:3 exposure induce a delta TEWL involves more than pure lipid extraction.
Does basic science knowledge correlate with clinical reasoning in assessments of first-year medical students?

Abstract Previous research has investigated the outcomes of Problem-based Learning (PBL), but little research has compared competencies in PBL and associated clinical reasoning skills with other competencies in medical education. We used results from formative and summative exams during the first block of medical education to investigate how the performance of beginning, undergraduate medical students on online clinical cases and additional clinical-reasoning questions related to their basic-science knowledge. We found a moderate correlation between clinical-reasoning and basic-science performance. However, the level of correlation suggests that distinct knowledge and skills are involved in clinical reasoning beyond those associated with basic-science knowledge.
Control with Limited Information

A long-standing open conceptual problem has been the following: How does “information” interact with control of a system, in particular feedback control, and what is the value of “information” in achieving performance objectives for the system through the exercise of control? In answering this question we have to remember that in contrast to a variety of communication settings, the issue of time-delay is of primary importance for control problems, especially control of systems which are unstable. We discuss various issues arising from these fundamental questions.
Stokes polarimetry imaging of dog prostate tissue

Prostate cancer is the second leading cause of death in the United States in 2009. Radical prostatectomy (complete removal of the prostate) is the most common treatment for prostate cancer, however, differentiating prostate tissue from adjacent bladder, nerves, and muscle is difficult. Improved visualization could improve oncologic outcomes and decrease damage to adjacent nerves and muscle important for preservation of potency and continence. A novel Stokes polarimetry imaging (SPI) system was developed and evaluated using a dog prostate specimen in order to examine the feasibility of the system to differentiate prostate from bladder. The degree of linear polarization (DOLP) image maps from linearly polarized light illumination at different visible wavelengths (475, 510, and 650 nm) were constructed. The SPI system used the polarization property of the prostate tissue. The DOLP images allowed advanced differentiation by distinguishing glandular tissue of prostate from the muscular-stromal tissue in the bladder. The DOLP image at 650 nm effectively differentiated prostate and bladder by strong DOLP in bladder. SPI system has the potential to improve surgical outcomes in open or robotic-assisted laparoscopic removal of the prostate. Further in vivo testing is warranted.
Deformation and Failure Modes of I-Core Sandwich Structures Subjected to Underwater Impulsive Loads

This article reports an experimental study carried out with the aim of quantifying performance and failure modes of sandwich structures when subjected to impulsive blast loading. In particular, performance enhancement with respect to solid panels of equal mass per unit area is assessed. Likewise, the optimal distribution of the mass per unit area in the design of sandwich structures is investigated by comparing the behavior of sandwich structures with various distributions of face sheets thickness. By employing a previously developed FSI experiment, the study confirmed that usage of sandwich structures is beneficial and that performance enhancements, in terms of maximum panel deflection, as high as 68% are possible. The study also confirms theoretical and computational analyses suggesting that use of soft cores maximizes the benefits. Another interesting aspect revealed by this work is that the level of enhancement is highly related to the applied normalized impulse. The same distribution of mass per unit area between face sheets resulted in different normalized maximum deflection. A better performance enhancement was achieved at lower impulses. Here again, failure modes and their sequence seem to be the directly related to this finding. The work here reported clearly reveals a number of important features in the study of lightweight structures and points out to the synergies between structure geometry, materials, manufacturing methods, and threat levels as manifested by the strength of the impulse. Further theoretical and computational studies accounting for experimentally observed failure modes and its interdependence with the fabrication methods is needed to achieve additional predictive capabilities.
Optimal mean-absolute-error filtering of gray-scale signals by the morphological hit-or-miss transform

The binary hit-or-miss transform is applied to filter digital gray-scale signals. This is accomplished by applying a union of hit-or-miss transforms to an observed signal's umbra and then taking the surface of the filtered umbra as the estimate of the ideal signal. The hit-or-miss union is constructed to provide the optimal mean-absolute-error filter for both the ideal signal and its umbra. The method is developed in detail for thinning hit-or-miss filters and applies at once to the dual thickening filters. It requires the output of the umbra filter to be an umbra, which in general is not true. A key aspect of the paper is the complete characterization of umbra-preserving union-of-hit-or-miss thinning and thickening filters. Taken together, the mean-absolute-error theory and the umbra-preservation characterization provide a full characterization of binary hit-or-miss filtering as applied to digital gray-scale signals. The theory is at once applicable to hit-or-miss filtering of digital gray-scale images through the three-dimensional (3-D) binary hit-or-miss transform.
Synthesis of Telechelic Polyisoprene via Ring-Opening Metathesis Polymerization in the Presence of Chain Transfer Agent

Telechelic polyisoprene was synthesized via the ring-opening metathesis polymerization (ROMP) of 1,5-dimethyl-1,5-cyclooctadiene (DMCOD) in the presence of cis-1,4-diacetoxy-2-butene as a chain transfer agent (CTA). This method afforded telechelic polymer in excellent yield, and the acetoxy groups were successfully removed to yield α,ω-hydroxy end-functionalized polyisoprene with potential for subsequent reactions. Efficient, quantitative incorporation of CTA was achieved, and NMR spectroscopy was utilized to confirm the chemical identity of the polymer end groups. Polymerization of discrete DMCOD monomer generated polyisoprene with excellent regioregularity in the polymer backbone. Successful ROMP of sterically challenging DMCOD in the presence of a CTA for chain end-functionalization was borne out through screening of a variety of Ru-based olefin metathesis catalysts.
Feasibility of gas-liquid chromatography for quantitative determination of aluminiumIII, GalliumIII, IndiumIII and BerylliumII Trifluoracetylacetonates

Abstract The gas Chromatographic behaviour of the trifluoracetyl-aeetonates (TFA's) of aluminiumIII, galliumIII, indiumIII, berylliumII and thallium has been studied and conditions for their complete separation found. The thallium derivative has not been characterised, but produces a chromatographic peak well separated from the others. Elution products have been collected and examined for comparison with the injected material. An over-all relative mean error of 2%, based on calibration data, demonstrates the feasibility of gas chromatography with a thermal conductivity detector for the quantitative determination of aluminium, gallium, indium and beryllium. Using conditions established for complete separations, it is possible to obtain the gas chromatographic analysis of any combination of up to 5 metals by a maximum of two runs which involve a change in column temperature only. The TFA's of scandiumII, chromiumIII, copperII, manganeseIII, zirconiumIV, hafniumIV, zincII and thoriumIV are studied, enabling a prediction of interference in this method.
State-based Medicaid costs for pediatric asthma emergency department visits.

Introduction The prevalence of childhood asthma in the United States increased from 8.7% in 2001 to 9.5% in 2011. This increased prevalence adds to the costs incurred by state Medicaid programs. We provide state-based cost estimates of pediatric asthma emergency department (ED) visits and highlight an opportunity for states to reduce these costs through a recently changed Centers for Medicare and Medicaid Services (CMS) regulation.
Dynamic interactions between transposable elements and their hosts

Genome sequencing is providing a wealth of information about where and when transposons can integrate into host genomes. This Review integrates findings from eukaryotes and bacteria about patterns of transposon activity, host defence mechanisms and the developmental timing of transposition. Transposable elements (TEs) have a unique ability to mobilize to new genomic locations, and the major advance of second-generation DNA sequencing has provided insights into the dynamic relationship between TEs and their hosts. It now is clear that TEs have adopted diverse strategies — such as specific integration sites or patterns of activity — to thrive in host environments that are replete with mechanisms, such as small RNAs or epigenetic marks, that combat TE amplification. Emerging evidence suggests that TE mobilization might sometimes benefit host genomes by enhancing genetic diversity, although TEs are also implicated in diseases such as cancer. Here, we discuss recent findings about how, where and when TEs insert in diverse organisms.
Effect of short-term exposure to polychlorinated biphenyls on first-pass metabolism of pentobarbital in rats.

The enhancement of the first-pass metabolism of orally administered pentobarbital in rats was examined after a 10-day exposure to food contaminated with polychlorinated biphenyls at 25 and 125 ppm. The degree of the first-pass effect and the influence of the polychlorinated biphenyl exposure were quantitated by comparing the areas under the Plasma concentration-time curves after oral and intravenous dosing in control and treated animals. By using the clearance model and assuming that pentobarbital was eliminated totally by liver metabolism, the experimentally determined oral availability was predicted adequately from both the oral and intravenous data. The enhanced first-pass effect was principally in the intrinsic clearance term, although liver blood flow rates also appeared to be enhanced in animals treated with polychlorinated biphenyls at 125 ppm.
Effect of Whole Cottonseeds and Calcium Salts of Long-Chain Fatty Acids on Performance of Lactating Dairy Cows

Holstein cows were assigned to diets containing no supplemental fat, supplemental fat from whole cottonseed, or supplemental fat from whole cottonseed plus Ca salts of fatty acids (Megalac). The TMR contained 46% forage and 54% concentrate or mixtures of concentrate and whole cottonseed on a DM basis and were fed from wk 3 through 44 of lactation. The mean fat content of the three diets was 3.0, 4.7, and 6.4% of DM for control, whole cottonseed, and whole cottonseed plus Ca salts of fatty acids, respectively. Supplemental fat increased NEL intake, percentage of milk fat, milk fat production, and rate of recovery of BW and body condition. Supplemental fat decreased milk protein production in early lactation, but not in late lactation. Addition of supplemental fat had no significant effect on ruminal concentration of VFA, NH3 N, or in situ digestibility of fiber. The proportion of unsaturated fatty acids in milk fat was increased with supplemental whole cottonseed or whole cottonseed plus Ca salts of fatty acids. During the first 3 mo of lactation, the proportion increased of fatty acids C14 or less, C16, and C18:2. Proportion of fatty acids C16:1 and C18:1 correspondingly decreased. The change in composition of milk fatty acids during early lactation is consistent with the use of body fat for milk synthesis.
The Pressure Broadening of HDO by O2, N2, H2, and He Between 100 and 600 K

Abstract Microwave transitions of HDO pressure-broadened by H 2 , O 2 , N 2 , and He were studied in the temperature region between 100 and 600 K. Measurements below 250 K were made in a collisionally cooled cell. Above this temperature a conventional equilibrium cell was used in the region where the HDO has nonnegligible vapor pressure. Significant variations in both the pressure-broadening parameter and its temperature variation were observed among the studied transitions. Below 150 K deviations from the power law ordinarily used to describe the temperature variations were observed.
Molecular Species on Nanoparticulate Anatase TiO2 Film Detected by Sum Frequency Generation: Trace Hydrocarbons and Hydroxyl Groups

Visible-infrared sum frequency generation (SFG) has been applied to probe molecular species on the surface of nanoparticulate anatase TiO2 films. A trace hydrocarbon film on the surface, not detected by Fourier transform infrared spectroscopy (FTIR), is easily sensed with SFG. The first direct observation of hydroxyl groups (-OH) on the TiO2 film surface by SFG is reported. A broad vibrational band with multiple peaks in the region of 3500−3800 cm-1 ascribed to OH groups reflects the surface heterogeneity of the material.
Rotor Bar Fault Monitoring Method Based on Analysis of Air-Gap Torques of Induction Motors

A robust method to monitor the operating conditions of induction motors is presented. This method utilizes the data analysis of the air-gap torque profile in conjunction with a Bayesian classifier to determine the operating condition of an induction motor as either healthy or faulty. This method is trained offline with datasets generated either from an induction motor modeled by a time-stepping finite-element (TSFE) method or experimental data. This method can effectively monitor the operating conditions of induction motors that are different in frame/class, ratings, or design from the motor used in the training stage. Such differences can include the level of load torque and operating frequency. This is due to a novel air-gap torque normalization method introduced here, which leads to a motor fault classification process independent of these parameters and with no need for prior information about the motor being monitored. The experimental results given in this paper validate the robustness and efficacy of this method. Additionally, this method relies exclusively on data analysis of motor terminal operating voltages and currents, without relying on complex motor modeling or internal performance parameters not readily available.
Fluorescence lifetime imaging of calcium using Quin-2

We describe the use of a new imaging technology, fluorescence lifetime imaging (FLIM), for the imaging of the calcium concentrations based on the fluorescence lifetime of a calcium indicator. The fluorescence lifetime of Quin-2 is shown to be highly sensitive to [Ca2+]. We create two-dimensional lifetime images using the phase shift and modulation of the Quin-2 in response to intensity-modulated light. The two-dimensional phase and modulation values are obtained using a gain-modulated image intensifier and a slow-scan CCD camera. The lifetime values in the 2D image were verified using standard frequency-domain measurements. Importantly, the FLIM method does not require the probe to display shifts in the excitation or emission spectra, which may allow Ca2+ imaging using other Ca2+ probes not in current widespread use due to the lack of spectral shifts. Fluorescence lifetime imaging can be superior to stationary (steady-state) imaging because lifetimes are independent of the local probe concentration and/or intensity, and should thus be widely applicable to chemical imaging using fluorescence microscopy.
The Role of “Antenna” Structure on Thin Oxide Damage from Plasma Induced Wafer Charging

A new, physically-based model has been developed to successfully explain the roles of device structure and plasma nonuniformity on charge damage. The model includes an equivalent circuit for the charging of MOS antenna structures exposed to a nonuniform plasma, and the use of SPICE, a circuit simulator, to correlate plasma measurement to breakdown measurements. The model is applied to analyze thin oxide damage in an O 2 magnetron plasma asher. The simulation results show good agreement with experimental damage data of “antenna” capacitors using ramp voltage breakdown measurements.
The case of the exploding perception

My brother, whom we may call Smith, was touring the London Wax Museum. In need of information, he approached a nearby bobby. But no sooner had he started "Excuse me, sir" than he realized, to his own chagrin and his companion's amusement, that he was speaking to a figure of wax. In such a case, we may say, one experiences an 'explosion' of perception: at first Smith sees (is perceptually presen ted with) a bobby, but then that perception 'explodes' and he sees a wax figure. (The imagery stems from Husserl.1) What are we to make of such perceptual 'explosions'? From considerations of the case above, together with some related cases, we shall draw a basic account of the intentionality of perception, an account of the basic intentional or phenomenological structure of seeing an individual. Our account permits an illuminating analysis of these cases of perceptual explosion and so finds motivation and support therein. So goes part I. In part II we pursue a more precise formulation of our characterization of these cases, finding motivation and support for a central part of Hintikka's logic of perception. And in part III we consolidate the results of parts I and II by drawing on Husserl's basic approach to intentionality. An appendix pursues technical aspects in a possible-worlds framework.
Heavily Doped p-Type PbSe with High Thermoelectric Performance: An Alternative for PbTe

PbSe was expected to have a smaller bandgap and higher thermalconductivity than PbTe. Instead, these values are about the same at high temperature leading to comparable thermoelectric figure of merit, with zT> 1 achieved in heavily doped p-type PbSe.
Diode-pumped gigahertz repetition rate femtosecond Cr:LiSAF laser

We report a low-cost, 1 GHz repetition-rate, diode-pumped, saturable Bragg reflectors mode-locked Cr:LiSAF laser, which generates nearly transform-limited 103-fs long pulses around 866 nm, with a record high peak power of 1.45 kW.
The yeast mitochondrial citrate transport protein : identification of the Lysine residues responsible for inhibition mediated by Pyridoxal 5'-phosphate

The present investigation identifies the molecular basis for the well-documented inhibition of the mitochondrial inner membrane citrate transport protein (CTP) function by the lysine-selective reagent pyridoxal 5′-phosphate. Kinetic analysis indicates that PLP is a linear mixed inhibitor of the Cys-less CTP, with a predominantly competitive component. We have previously concluded that the CTP contains at least two substrate binding sites which are located at increasing depths within the substrate translocation pathway and which contain key lysine residues. In the present investigation, the roles of Lys-83 in substrate binding site one, Lys-37 and Lys-239 in substrate binding site two, and four other off-pathway lysines in conferring PLP-inhibition of transport was determined by functional characterization of seven lysine to cysteine substitution mutants. We observed that replacement of Lys-83 with cysteine resulted in a 78% loss of the PLP-mediated inhibition of CTP function. In contrast, replacement of either Lys-37 or Lys-239 with cysteine caused a modest reduction in the inhibition caused by PLP (i.e., 31% and 20% loss of inhibition, respectively). Interestingly, these losses of PLP-mediated inhibition could be rescued by covalent modification of each cysteine with MTSEA, a reagent that adds a lysine-like moiety (i.e. SCH2CH2NH3+) to the cysteine sulfhydryl group. Importantly, the replacement of non-binding site lysines (i.e., Lys-45, Lys-48, Lys-134, Lys-141) with cysteine resulted in little change in the PLP inhibition. Based upon these results, we conducted docking calculations with the CTP structural model leading to the development of a physical binding model for PLP. In combination, our data support the conclusion that PLP exerts its main inhibitory effect by binding to residues located within the two substrate binding sites of the CTP, with Lys-83 being the primary determinant of the total PLP effect since the replacement of this single lysine abolishes nearly all of the observed inhibition by PLP.
CHARGE-COUPLED DEVICE SPECKLE OBSERVATIONS OF BINARY STARS WITH THE WIYN* TELESCOPE. V. MEASURES DURING 2001-2006

A total of 1067 speckle observations of 345 binary stars are presented. Of these, 161 are double stars first resolved by Hipparcos, 17 are resolved for the first time in the observations presented here, and 21 are stars previously discovered by our program and reported in earlier papers in the series. In 947 cases, a magnitude difference is reported along with the relative astrometry. When comparing to systems with very well-known orbits, we find that the root mean square (rms) deviation in separation residuals is 2.81 ± 0.28 mas, and the rms deviation in position angle residuals is 0.88 ± 0.07°. The magnitude difference measures show no significant deviation from Hipparcos photometry, and have average standard deviation of approximately 0.10 mag as judged from repeat observations. Five important systems discovered by Hipparcos are discussed.
Microprocessor-controlled Data Acquisition System for Pulsed Laser Spectroscopy

Pulsed laser systems produce spectroscopic signals with large shot-to-shot variations, short durations, and low duty cycles. The most satisfactory signal processing system is gated integration, with pulse averaging and ratioing to a reference signal. We describe a system based on commercially available modular integrators and a hobbyist microcomputer which performs the averaging and ratioing digitally. System performance is equal to or better than that of fully analog systems, while the cost is low and construction is straightforward.
Impaired in vivo insulin clearance in patients with severe target-cell resistance to insulin

The concentration of insulin in plasma is determined by both its rate of secretion and its rate of clearance from the plasma compartment. The effect of marked insulin resistance on insulin clearance in vivo has not been determined in man. We have employed the euglycemic insulin clamp technique to measure insulin sensitivity and insulin clearance in 16 control subjects and in 4 subjects with marked target-cell resistance to insulin. Two insulin-resistant patients had reduced receptor concentration on peripheral mononuclear cells, and two patients had normal receptor number and affinity. During 80-mU/m2/min insulin clamp studies, the clearance rate in each insulin-resistant patient was lower than that in any controls; the mean insulin clearance rate was 511 +/- 74 ml/m2/mon in control subjects and 205 ml/m2/min (P less than 0.001) in insulin-resistant patients. These findings demonstrate an association between marked target-cell resistance to insulin and impaired in vivo insulin clearance, and suggest an important role for receptor-mediated pathways in insulin clearance in vivo.
Evolutionary diversification of the multimeric states of proteins

One of the most striking features of proteins is their common assembly into multimeric structures, usually homomers with even numbers of subunits all derived from the same genetic locus. However, although substantial structural variation for orthologous proteins exists within and among major phylogenetic lineages, in striking contrast to patterns of gene structure and genome organization, there appears to be no correlation between the level of protein structural complexity and organismal complexity. In addition, there is no evidence that protein architectural differences are driven by lineage-specific differences in selective pressures. Here, it is suggested that variation in the multimeric states of proteins can readily arise from stochastic transitions resulting from the joint processes of mutation and random genetic drift, even in the face of constant directional selection for one particular protein architecture across all lineages. Under the proposed hypothesis, on a long evolutionary timescale, the numbers of transitions from monomers to dimers should approximate the numbers in the opposite direction and similarly for transitions between higher-order structures.
Laura Ingalls Wilder's Little Town: Where History and Literature Meet

As Laura Ingalls Wilder anticipated, her widely loved stories of her prairie childhood have become much more than a nostalgic blend of myth, memories, and autobiography. As John Miller reveals, they have much to tell us about the historical realities of day-to-day living and attitudes in the nineteenth century. History and literature are closely intertwined, Miller contends. Here he illustrates how Wilder's novels enhance our understanding of history and how, simultaneously, a historical perspective framed Wilder's fiction. He shows how Wilder interwove content and form to produce a sentimental and compelling yet nuanced and believable picture of family life on the agricultural frontier.
A preliminary study of the kalina power cycle in connection with a combined cycle system

Combined cycle systems have been recognized as efficient power systems in which exhaust gas from the topping cycle provides the available energy to the bottoming cycle. Since most heat sources available to the bottoming cycle are sensible-heat sources, there may be a better thermal match, and an increase thermodynamic efficiency, on reducing the entropy generation of the simple combined cycle. To increase the efficiency of the Rankine cycle working with sensible heat, two conventional methods have been proposed: one is to incorporate a multipressure boiler; the other is to implement a supercritical cycle. An alternative method is to use a multicomponent working fluid boiling at a variable temperature with a change in the liquid composition of the components, and yielding a better thermal match with the sensible-heat source than the constant temperature boiling process. The Kalina cycle is an implementation of this concept, where ammonia/water mixtures are used as the working fluid. The purpose of this study is to conduct a preliminary study of the Kalina power cycle system in connection with a combined cycle system, comparing the Kalina cycle and the Rankine cycle. This study is performed using new thermodynamic properties of ammonia/water mixtures developed by the authors.
The Influence of Metallic Cations on the Penetration of the Egg Shell Membranes by Pseudomonas Fluorescens

Abstract BACTERIAL spoilage of shell eggs is a serious economic problem as well as a public health hazard. As evident by extensive literature reviews by Hadley and Caldwell (1916) and Lorenz et al. (1952), the problem of microbial infection of shell eggs has been recognized and investigated over a period of years going back into the 1700’s. However, a basic understanding of factors which permit bacterial entry into the shell egg still needs elucidation. The major source of bacteria in shell eggs has been found to originate from external contamination rather than from an internal infection of the egg contents (Stuart and McNally, 1943). They reported that the shell and shell membranes were the major barriers to bacterial penetration into the egg. The shell membranes were reported to form a mechanical restraint against microorganisms by Walden et al. (1956). Kraft et al. (1958) reported that the shell without its membranes did …
The interaction of 1-anilino-8-naphthalenesulfonate with lipids: a nuclear magnetic resonance study.

Abstract The proton magnetic resonance (PMR) and phosphorus magnetic resonance (PhMR) spectra of egg phosphatidylcholine in the presence of 1-anilino-8-naphthalenesulfonate (ANS) have been studied. At low ratios of ANS to phospholipid, the spectra indicate that ANS molecules are in the lipid interface region where they interact with the head-group protons. ANS also penetrates into the hydrocarbon region to some extent. As the ANS/phospholipid ratio approaches one, a significant splitting of the head-group signal occurs. This splitting is associated with head-group signals from inner and outer molecules of the phospholipid vesicles. As the ANS/phospholipid ratio is further increased, a gel phase often occurs. The spectra for this gel phase suggest a highly mobile head-group. Further ANS addition results in a PMR spectrum suggestive of ANS—phospholipid micelle formation. The results for a phospholipid—cholesterol complex and for the total lipid extract from a cell membrane show that the ANS effect is more complicated in these cases.