cssi/SciSciGPT-SciSciNet huggingface dataset:
FIZ1 is part of the regulatory protein complex on active photoreceptor-specific gene promoters in vivo.

FIZ1 (Flt-3 Interacting Zinc-finger) is a broadly expressed protein of unknown function. We reported previously that in the mammalian retina, FIZ1 interacts with NRL (Neural-Retina Leucine-zipper), an essential transcriptional activator of rod photoreceptor-specific genes. The concentration of FIZ1 in the retina increases during photoreceptor terminal maturation, when two key transcription factors NRL and CRX (Cone-Rod Homeobox) become detectable on the promoters of photoreceptor-specific genes (i.e. Rhodopsin, Pde6b). To determine if FIZ1 is involved in regulating CRX-mediated transcriptional activation, we examined FIZ1 subcellular location in mouse neural retina, its ability to interact with CRX, and its association with CRX/NRL target genes. FIZ1 is present in the nucleus of adult photoreceptors as well as other retinal neurons as shown by transmission electron microscopy with nano-gold labeling. FIZ1 and CRX were co-precipitated from retinal nuclear extracts with antibodies to either protein. Chromatin immunoprecipitation (ChIP) assays revealed that FIZ1 is part of the protein complex on several rod and cone gene promoters, within photoreceptor cells of the mouse retina. FIZ1 complexes with CRX or NRL on known NRL- and CRX-responsive elements, as shown by electrophoretic mobility shift assays with FIZ1 antibody. FIZ1 can directly bind to CRX, as demonstrated using yeast two-hybrid and GST pull-down assays. Co-transfection assays demonstrated that FIZ1 increases CRX-mediated activation of Opsin test promoters. Quantitative ChIP analysis revealed an increased association of FIZ1 with the Rhodopsin promoter in adult (P-25) neural retina versus immature (P-3) neural retina. The quantity of transcriptionally active RNA Polymerase-II within the Rhodopsin gene (Rho) was significantly increased in the adult neural retina, compared to the immature retina. FIZ1 directly interacts with CRX to enhance CRX's transactivation activity for target genes. Developmentally, in neural retina tissue, the increased association of FIZ1 with CRX target genes corresponds to an increased association of transcriptionally active Pol-II within the Rho gene. Together with previous findings, our results suggest that FIZ1 may act as a transcriptional co-regulator of photoreceptor-specific genes, recruited by at least two photoreceptor-specific transcription factors, CRX and NRL. Further studies are underway to elucidate the exact role of FIZ1 in photoreceptor gene expression, development and maintenance.
The Relationship Between Information System Planning Sophistication and Information System Success: An Empirical Assessment*

Literature on information systems (IS) planning implicitly assumes that a sophisticated IS planning process leads to greater IS success. This paper questions the exclusive reliance on this traditional belief. Instead, because IS planning requires significant organizational resources, prior IS success is essential to convince top management of the importance of IS planning sophistication. Therefore, IS success may influence IS planning sophistication. Several theoretical arguments are advanced in support of this explanation. Data from a survey of 236 academic institutions are used to empirically assess the two alternative directions of the relationship between IS planning sophistication and IS success. Four structural models including the alternative causal directions are evaluated. Two of these models are supported. Together, they imply that for a high level of IS planning sophistication, either the previous ISs should have been successful or the organization should possess advanced information technology capabilities. Thus, empirical results suggest that the explanation presented here (i.e., IS success facilitates IS planning sophistication) provides an equally good alternative to the more traditional explanation (i.e., IS planning sophistication facilitates IS success).
Effect of complex flow kinematics on the molecular orientation distribution in injection molding of liquid crystalline copolyesters

Abstract Wide-angle X-ray scattering (WAXS) is used to probe the molecular orientation in steady isothermal complex channel flows (in situ) and in injection molded plaques (ex situ) of a new, low-cost aromatic copolyester based on the mesogen 4,4′-dihydroxy-α-methylstilbene (DHαMS). Complex orientation states arise from the competition of inhomogeneous mixed shear and extension in isothermal flows. Slit-contraction flows lead to a significant but temporary increase in the average degree of molecular orientation, suggesting that this polymer is of the ‘shear-tumbling’ type. Conversely, bimodal orientation states are observed in slit-expansion flows, where transverse extension leads to a strong reduction in the average degree of molecular orientation along the flow direction. Similar bimodal orientation states are observed in injection molded plaques, suggesting that these kinematic concepts translate rather directly to the more complex transient non-isothermal case of injection molding. Variations in orientation state induced by changes in plaque thickness may be rationalized by systematic changes in the relative importance of shear and extension. These results suggest a complementary perspective on ‘skin-core’ morphologies in liquid crystalline polymer moldings, and provide a clear conceptual link between more fundamental studies in isothermal flows and structure development during processing.
Modulation by S-Adenosyl-L-Methionine of Hepatic Na+, K+-ATPase, Membrane Fluidity, and Bile Flow in Rats with Ethinyl Estradiol-Induced Cholestasis

Structural and functional changes in the surface membranes of hepatocytes play a pivotal role in the induction and reversion of some forms of drug-induced cholestasis. To elucidate the mechanism by which S-adenosyl-L-methionine (SAMe) leads to a partial reversion of bile flow impairment caused by ethinyl estradiol (EE), female Sprague-Dawley rats were given oral doses of EE (5 mg per kg per day, for 3 days) with and without simultaneous administration of SAMe (25 mg per kg, 3 times per day, for 3 days). Na+, K+-ATPase activity and membrane microviscosity as measured by fluorescent polarization were assayed in isolated liver plasma membranes (LPMs). SAMe administration to normal and EE-treated rats resulted in a marked increase in Na+, K+-ATPase activity and LPM fluidity. EE alone did not cause any change in the physicochemical properties of the LPMs. Hepatic Mg2+-ATPase and y-glutamyl transpeptidase activities were not affected by SAMe alone but increased when SAMe was given together with EE. These data indicate that the interaction of in vivo administered SAMe with hepatocyte plasmalemma and its effect on lipid fluidity and enzymes of the LPMs showed a high specificity and an inverse relationship between Na+, K+-ATPase activity and fluorescence polarization values. Furthermore, modulation of hepatic Na+, K+-ATPase was associated with SAMe-induced protection against bile flow impairment due to EE; however, it was not the causative factor for EE-induced cholestasis under the experimental conditions. These findings suggest that changes in surface membrane structure and function might account in part for the reversal by SAMe of EE-induced impairment of bile secretory function.
Level of Emotional Arousal in Laboratory Training.

In order to study the level of emotional arousal produced by laboratory training, data from the highest level of a four-T-Group laboratory were tested against the following stress conditions from perceptual isolation investigations: 6 hours, 8 hours, and 24 hours. Data were collected on a self-administering adjective check list which provided scores on anxiety, depression, and hostility. Anialyses of covariance were conducted, using prestress scores as covariates to provide statistical control in the absence of experimental matching of subjects.Laboratory training mean scores on anxiety, depression, and hostility for the four T Groups at the highest level (stress condition) were significantly lower than the mean scores from the stress condition of the three perceptual isolation studies. Comparison of the poststress frequency distributions for the four samples indicates that no scores occur for laboratory training at or beyond the point traditionally accepted as deviant (T score of 70 on the standardizatio...
Correlation of Arteriographic and Pathologic Findings in the Coronary Arteries in Man

The accuracy or clinical coronary arteriography was studied by comparing pathologic and radiologic findings in 130 major coronary arterial segments obtained from 13 individuals at autopsy. Abnormal roentgenographic findings were verified by pathologic examination; however, half of the segments that appeared normal or minimally involved radiologically demonstrated more severe changes at autopsy.
Raman spectrometry study of phonon anharmonicity of hafnia at elevated temperatures

Raman spectra of monoclinic hafnium oxide $({\text{HfO}}_{2})$ were measured at temperatures up to 1100 K. Raman peak shifts and broadenings are reported. Phonon dynamics calculations were performed with the shell model to obtain the total and partial phonon density of states, and to identify the individual motions of Hf and O atoms in the Raman modes. Correlating these motions to the thermal peak shifts and broadenings, it was found that modes involving changes in oxygen-oxygen bond length were the most anharmonic. The hafnium-dominated modes were more quasiharmonic and showed less broadening with temperature. Comparatively, the oxygen-dominated modes were more influenced by the cubic term in the interatomic potential than the hafnium-dominated modes. An approximately quadratic correlation was found between phonon-line broadening and softening.
Equilibrium analysis of volatility clustering

Abstract Volatility clustering is a pervasive feature of equity markets. This article studies volatility clustering in an equilibrium setting by generalizing the CRRA and CARA representative agent models of finance. In equilibrium, the market portfolio follows a volatility regime-switching process in which the volatility level is determined by the agent's local risk aversion. Using monthly data, the empirical tests reveal that at least four volatility regimes are necessary to fit the data. While one of the models explains the GARCH effects in the data, an analysis of the Euler equation pricing errors suggests that both models are likely misspecified. Since the models can be used to closely approximate any state-independent utility function, it is doubtful that there exists any representative agent equilibrium (with state-independent utility) that is consistent with the data. An equivalent interpretation is that the market portfolio price process is not a diffusion process of the type studied by Bick [Bick, A., On viable diffusion price processes of the market portfolio, J. Finance 45 (1990) 673–689] and He and Leland [He, H., Leland, H., On equilibrium asset price processes, Rev. Financ. Stud. 6 (1993) 593–617].
Critical behavior of dilute NaCl in H2O

Abstract The compositions of the saturated vapor and liquid phases are measured for the system NaCl-H2O at 380°C, which is close to the critical point of pure water. The shape of the phase equilibrium curve is classical, which confirms a conclusion reached earlier on the basis of less accurate data. This implies that the long-range forces introduced by the NaCl suppress the non-classical effects present in pure H2O. An empirical equation of a classical type fits these data.
Kinematics of level terrestrial and underwater walking in the California newt, Taricha torosa

Salamanders are acknowledged to be the closest postural model of early tetrapods and are capable of walking both in a terrestrial environment and while submerged under water. Nonetheless, locomotion in this group is poorly understood, as is underwater pedestrian locomotion in general. We, therefore, quantified the movements of the body axis and limbs of the California newt, Taricha torosa, during steady-speed walking in two environments, both of which presented a level surface: a treadmill and a trackway that was submerged in an aquarium. For treadmill walking at a relative speed of 0.63 snout-vent lengths (SVL)/sec, newts used a diagonal couplets lateral sequence walk with a duty factor of 77%. In contrast, submerged speeds were nearly twice as fast, with a mean of 1.19 SVL/sec. The submerged gait pattern was closer to a trot, with a duty factor of only 41%, including periods of suspension. Environment appears to play a critical role in determining gait differences, with reduction of drag being one of the most important determinants in increasing duration of the swing phase. Quantitative analysis of limb kinematics showed that underwater strides were more variable than terrestrial ones, but overall were strikingly similar between the two environments, with joint movement reversals occurring at similar points in the step cycle. It is suggested that the fundamental walking pattern appears to function well under multiple conditions, with only minor changes in motor control necessary.
Morris, Michael. (2007). An Introduction to the Philosophy of Language. Cambridge UK, Cambridge University Press. v + 326 pp. $89.00, hardcover, ISBN 0-521-60311-0

An Introduction to the Philosophy of Language by Michael Morris is a valuable resource for students studying linguistics and for readers who are interested in the nature of language. The book intro...
Paratesticular rhabdomyosarcoma in a neonate

Abstract A 13-day-old boy presented with left scrotal tumor and coronary hypospadias. Left radical orchiectomy was performed. Histological diagnosis was embryonal-type paratesticular rhabdomyosarcoma. To the authors' knowledge, this is the first reported case of paratesticular rhabdomyosarcoma in a neonate in English-language literature. Also, theassociation of a testicular tumor with hypospadias has not been noted. J Pediatr Surg 35:605-606. Copyright © 2000 by W.B. Saunders Company.
Incidence of anatomic variants in Guyon's canal

This study was undertaken to determine the frequency of variations involving the anatomy of Guyon's canal. A series of 58 wrist dissections revealed a 22.4% incidence of anomalous muscles and a 1.7% incidence of anomalous nerve paths. The relationship of the deep branch of the ulnar artery to the superficial and deep branches of the ulnar nerve was found to vary. Accessory muscles passing through Guyon's canal are common, and are often found bilaterally. Aberrant branching of the ulnar nerve in this area is rare.
Defending Modernity? High Politics, Feminist Anti-Modernism, and the Place of Gender

In the wake of 9/11 and the subsequent struggles in Afghanistan and Iraq, gender has become even more obviously important for understanding contemporary world politics, including armed conflict and war. Gender is an explicit structuring principle of contemporary conflicts between Western powers and an Islamist fundamentalism energized by opposition to the freedoms enjoyed by Western women and committed to the imposition of a version of sharia that explicitly denies to women rights equal to men's.
The impact of dermatology consultation on diagnostic accuracy and antibiotic use among patients with suspected cellulitis seen at outpatient internal medicine offices: a randomized clinical trial.

Importance Cellulitis is a common and costly problem, often diagnosed in the outpatient setting. Many cutaneous conditions may clinically mimic cellulitis, but little research has been done to assess the magnitude of the problem. Objective To determine if obtaining dermatology consultations in the outpatient primary care setting could assist in the diagnosis of pseudocellulitic conditions and reduce the rate of unnecessary antibiotic use. Design, Setting, and Participants Nonblinded randomized clinical trial of competent adults who were diagnosed as having cellulitis by their primary care physicians (PCPs), conducted at outpatient internal medical primary care offices affiliated with a large academic medical center. Interventions Outpatient dermatology consultation. Main Outcomes and Measures Primary outcomes were final diagnosis, antibiotic use, and need for hospitalization. Results A total of 29 patients (12 male and 17 female) were enrolled for participation in this trial. Nine patients were randomized to continue with PCP management (control group), and 20 patients were randomized to receive a dermatology consultation (treatment group). Of the 20 patients in the dermatology consultation group, 2 (10%) were diagnosed as having cellulitis. In the control group, all 9 patients were diagnosed as having cellulitis by PCPs, but dermatologist evaluation determined that 6 (67%) of these patients had a psuedocellulitis rather than true infection. All 9 patients (100%) in the control group were treated for cellulitis with antibiotics vs 2 patients (10%) in the treatment group ( P Conclusions and Relevance Dermatology consultation in the primary care setting improves the diagnostic accuracy of suspected cellulitis and decreases unnecessary antibiotic use in patients with pseudocellulitic conditions. Obtaining an outpatient dermatology consultation may be a cost-effective strategy that improves quality of care. Trial Registration clinicaltrials.gov Identifier:NCT01795092
Collective Expansion in High-Energy Nuclear Collisions - The search for the partonic EOS at RHIC

We discuss recent results from RHIC. Issues of energy loss and partonic collectivity from A u + A u collisions at S NN = 200 GeV are the focus of this paper. We propose a path toward the understanding of the partonic Equation of State in high energy nuclear collisions.
Distant Learning Students: A Comparison with Traditional Studies

This study was undertaken to determine if distant learning students are different from traditional students as concerns their motivation, wants, and what they receive from their educational experience. The differences between these two groups was found to be small. The major difference uncovered was that the distant learning students are less dissatisfied with what they receive from the educational experience. This could imply that the distant learning student is learning more of the course material due to less frustration. The major differences in types of students is between those who are motivated by a desire to learn the material versus those who are taking the course to satisfy some higher requirement, such as getting a degree from the institution. However, the motivation for taking the course is nearly identical for distant learning students and traditional students. Educational emphasis and presentation was not shown to demand modification to accommodate distant learning students. The distant learn...
Hydrogen Bond Assisted Assembly of Well-Ordered Polyhedral Oligomeric Silsesquioxane–Block Copolymer Composites

Well-ordered block copolymer nanocomposites with d-spacings as small as 15 nm and additive loadings greater than 70 mass % were formed upon blending functionalized polyhedral oligomeric silsesquioxanes (POSS) additives into disordered block copolymers containing poly(ethylene oxide) (PEO) as one of the blocks. The POSS additives were functionalized with maleamic acid or aminophenyl groups as ligands to enable selective hydrogen bonding with the PEO chains. The selective interaction of the additives with the PEO chains caused microphase separation of the block copolymer leading to formation of well-ordered morphologies as evidenced by small-angle X-ray scattering. Further addition of the additive induced transitions between cylindrical and spherical morphologies. While both ligands induced order, the maleamic acid ligand enabled higher levels of incorporation of POSS cages into the PEO phases of the composite. Differential scanning calorimetry and wide-angle X-ray diffraction were performed to confirm comp...
Study of ultrafine displacements by microdifferential holography

STUDY OF ULTRAFINE DISPLACEMENTS BY MICRODIFFERENTIAL HOLOGRAPHYMark Sharnoff and Hungyi LinUniversity of Delaware, Department of Physics and AstronomySharp Laboratory, Newark, DE 19716-2570ABSTRACTWe describe a multiplexed form of phase—step interferometrysuitable for measuring and mapping deformations at the nanometric andsubnanometric level. Differential holograms of an object under stressare obtained by twin exposures sandwiched about a phase shift of1(1+26) or 1(1-26) radians in the reference wave. Two reference wavesare employed, and a conjugate pair of such holograms is recordedsimultaneously, with ISlc<1, on high-resolution medium. Thedifferential holograms of the pair are read individually. Theinterferograms reconstructed therefrom, once placed into accurateregistration, are electronically reconstituted into an image in whichsmall displacements are mapped linearly into intensity. An improvedregistration method used in the present work permits us to mapdisplacements.smaller than 0.4 nanometer within the optically sparsespecimens that we use for calibration.1. INTRODUCTIONHolographic interferometry maps the displacement or strain of anobject under test by means of the fringes that appear in its images.The fringes are contours of constant displacement, typically moduloone half optical wavelength. Once the fringes have been properlyindexed, interpolation permits calculation of the total displacementof any object point with precision that may attain a hundredth of awave[1). Interpolation is awkward for objects or strain fields richin fine detail, and it fails entirely when the maximum displacement issmaller than half a wavelength. A number of strain fields may fallinto this class, including those of VLSI circuits and nanostructuraldevices produced microlithographically, and those associated with manyprocesses of interest in biology. To handle such situations we havedeveloped a holographic system having both high resolution in theimagingdomain and ultrahigh sensitivity in the interferometric
Cellular manufacturing systems modeling: The Petri net approach

Abstract The two requirements for automating a cellular manufacturing system are making the manufacturing cell control system efficient and decreasing its response time. Cell control algorithms can be generated from a system model that can truly represent the system. In this research, Petri nets are used to model and to represent the dynamic behavior of an unmanned cell's operations. These nets fully describe both the operations of a specific cell and the detailed operations within the cell. Petri nets show the pull system control, the decision-making points in the system (represented by the transitions that have inhibitor arcs attached), and the flexibility of part-type changes (allowed by mutual exclusion).
The type III TGFβ receptor regulates filopodia formation via a Cdc42-mediated IRSp53-N-WASP interaction in epithelial cells

Cell adhesion and migration are tightly controlled by regulated changes in the actin cytoskeleton. Previously we reported that the TGFβ (transforming growth factor β) superfamily co-receptor, TβRIII (type III TGFβ receptor; also known as βglycan), regulates cell adhesion, migration and invasion, and suppresses cancer progression, in part, through activation of the small GTPase Cdc42 (cell division cycle 42), and Cdc42-dependent alterations to the actin cytoskeleton. In the present study we demonstrate that TβRIII specifically promotes filopodial formation and extension in MCF10A and HMEC (human mammary epithelial cell) mammary epithelial cells. Mechanistically, cell-surface TβRIII and Cdc42 co-localize to filopodial structures and co-complex in a β-arrestin2-dependent, and a TβRI/TβRII-independent manner. The β-arrestin2-mediated interaction between TβRIII and Cdc42 increases complex formation between the Cdc42 effectors IRSp53 with N-WASP (neuronal Wiskott-Aldrich syndrome protein) to increase filopodial formation. We demonstrate a function link between filopodial structures and epithelial cell adhesion as regulated by the TβRIII-Cdc42 interaction. The present studies identify TβRIII as a novel regulator of IRSp53/N-WASP via Cdc42 to regulate filopodial formation and cell adhesion.
Mentoring New Teachers: Implications for Leadership Practice in an Urban School:

This article focuses on leadership practices and teacher mentoring in an urban school context. A central perspective of this discussion is the role of the urban school principal in implementing and...
Transport Limitations of Nitric Oxide Inhibition of Platelet Aggregation under Flow

Nitric oxide (NO) inhibits platelet aggregation at and near the site of a vascular injury by upregulation of cyclic guanosine monophosphate, which reduces the dimerization of the integrin αIIbβ3. The magnitude of NO flux from the vessel wall and the NO concentration that is necessary to inhibit platelet aggregation under physiological flow conditions is unknown. In this study, a NO releasing polymer, diazeniumdiolated dibutylhexanediamine, was integrated into a microfluidic flow assay to determine the relationship between NO wall flux and collagen mediated platelet adhesion, activation and aggregation. A NO flux equal to or greater than 2.5 × 10−10 mol cm−2 min−1 was found to abrogate aggregation, but not initial platelet adhesion, on collagen at 200 and 500 s−1 as effectively as the αIIbβ3 antagonist abciximab. The dynamic range of NO fluxes found to induce measurable inhibition of platelet aggregation spanned from 0.33 × 10−10 to 2.5 × 10−10 mol cm−2 min−1 at 200–500 s−1. These fluxes correspond to near-wall NO concentrations of 3–90 nM based on a computational model of NO transport. The model predicts that NO concentration in the platelet rich layer near the wall is kinetically limited, while NO penetration into the lumen is mass transfer limited.
Central stimulating properties of some terpenones

Dicyclic and monocyclic terpenones were studied for their central stimulating effects. Differences in the convulsive actions were noted for the two types of terpenones and a structure-activity relationship is postulated to explain variations in toxicity. Tests for arousal of hexobarbitalized mice, respiratory stimulation, running movement, and total movement did not indicate any consistent differences between the two groups. The possible advantage of some terpenones as convulsants for the screening of petit mal anticonvulsants is studied and discussed.
Mechanical loss in fibers for low noise pendulums

We describe measurements of the quality factors of resonances in fibers made of tungsten, silicon, sapphire, and fused quartz. The silicon and sapphire samples exhibited losses consistent with thermoelastic damping. Our fused quartz samples showed excess loss at some frequencies. The tungsten fibers had quality factors much lower than the thermoelastic prediction at all frequencies that we measured. We calculate the expected Brownian motion of pendulums made with similar fibers.
Na+ and K+ fluxes stimulated by Na+-coupled glucose transport: Evidence for a Ba2+-insensitive K+ efflux pathway in rabbit proximal tubules

Addition of glucose or the nonmetabolizable analogue alpha-methyl-D-glucoside to rabbit proximal tubules suspended in a glucose- and alanine-free buffer caused a sustained increase in intracellular Na+ content (+43 +/- 7 nmol.(mg protein)-1) and a concomitant but larger decrease in K+ content (-72 +/- 11 nmol.(mg protein)-1). A component of the net K+ efflux was Ba2+ insensitive, and was inhibited by high (1 mM) but not low (10 microM) concentrations of the diuretics furosemide and bumetanide. The increase in intracellular Na+ content is consistent with the view that the increased rates of Na+ and water transport seen in the proximal tubule in the presence of glucose can be attributed (at least in part) to a stimulation of basolateral pump activity by an increased [Na+]i.
Effect of Molecular Weight, Acid, and Plasticizer on the Physicochemical and Antibacterial Properties of β‐Chitosan Based Films

Effects of chitosan molecular weight (1815 and 366 kDa), type of acid (1% acetic, formic, and propionic acid, or 0.5% lactic acid) and plasticizer (0, 25% glycerol or sorbital w/w chitosan) on the mechanical, water barrier, and antibacterial properties of β-chitosan films were investigated. Tensile strength (TS) of high molecular weight (Hw) films was 53% higher than that of low molecular weight (Lw) ones, acetate, and propionate films had the highest TS (43 and 40 MPa) among tested acids, and plasticizer-reduced film TS 34%. Film elongation at break (EL) was higher in Hw films than in Lw ones, in which formate and acetate films were the highest (9% and 8%, respectively), and plasticizer increased the film EL 128%. Molecular weight of chitosan did not influence water vapor permeability (WVP) of the films. Acetate and propionate films had lower WVP than other acid types of films, and plasticizer increased film WVP about 35%. No difference was found between glycerol and sorbitol films in terms of film mechanical and water barrier properties. Lw β-chitosan films showed significant antibacterial activity against E. coli and L. innocua. This study demonstrated that β-chitosan films are compatible to α-chitosan films in physicochemical properties and antibacterial activity, yet with simple sample preparation.
Molecular cloning, encoding sequence, and expression of vaccinia virus nucleic acid-dependent nucleoside triphosphatase gene.

Abstract A rabbit poxvirus genomic library contained within the expression vector lambda gt11 was screened with polyclonal antiserum prepared against vaccinia virus nucleic acid-dependent nucleoside triphosphatase (NTPase)-I enzyme. Five positive phage clones containing from 0.72- to 2.5-kilobase-pair (kbp) inserts expressed a beta-galactosidase fusion protein that was reactive by immunoblotting with the NTPase-I antibody. Hybridization analysis allowed the location of this gene within the vaccinia HindIIID restriction fragment. From the known nucleotide sequence of the 16-kbp vaccinia HindIIID fragment, we identified a region that contains a 1896-base open reading frame coding for a 631-amino acid protein. Analysis of the complete sequence revealed a highly basic protein, with hydrophilic COOH and NH2 termini, various hydrophobic domains, and no significant homology to other known proteins. Translational studies demonstrate that NTPase-I belongs to a late class of viral genes. This protein is highly conserved among Orthopoxviruses.
Managing dairy cattle for fertility.

Abstract High reproductive efficiency in dairy herds is dependent upon good management. Accurate records on the reproductive status of each cow are essential for efficient management and for diagnoses by veterinarians. No single nutrient is required specifically for reproduction alone. The nutritive requirements for reproduction should be adequately supplied. Current evidence fails to show any relationship between infertility and feeding urea at recommended levels. Following parturition, rebreeding should be delayed until the reproductive tract has fully recovered from pregnancy. Without postpartum examinations to confirm that cows are ready to breed, higher fertility may be expected by waiting 60 days or more after calving before rebreeding. Cows and heifers should be observed carefully at least twice daily for standing estrus, and should be inseminated near the end of standing estrus. Faulty estrus detection and breeding at the wrong time during estrus constitute prime management errors that limit reproductive efficiency. Current progress in hormone assays should provide clinical tests for future use in managing cattle reproduction and in diagnosis and treatment of infertility. It is possible that estrus and ovulation control may enable mass handling of cows at breeding time.
Laser-Induced Fluorescence Studies of Oxygen Transfer Across Unsheared Flat and Wavy Air−Water Interfaces

Oxygen transfer across unsheared flat and wavy air−water interfaces is studied using a laser-induced fluorescence (LIF) technique. The LIF technique measures the dissolved oxygen (DO) concentration through oxygen quenching of the fluorescence from dissolved pyrene butyric acid (PBA). Fluorescence intensities in a two-dimensional plane in the water near the interface are imaged (instantaneously) with a high-resolution CCD camera. A series of image-processing and image-analysis steps corrects the intensities for variations in the fluorescence attributed to factors other than oxygen quenching and transforms the corrected fluorescence intensities into spatial measurements of the DO concentration. Near-interface vertical oxygen concentration profiles and two-dimensional concentration fields result. Estimates were obtained for the concentration boundary layer thickness, δc, and the mass transfer coefficient, kl, along flat and wavy interfaces. A severalfold increase in mass transfer rates is observed for an uns...
Environmental correlates of underage alcohol use and related problems of college students.

Abstract Background: Underage alcohol use is a major contributor to morbidity and mortality in adolescents and young adults. This study examined drinking levels and ensuing problems among college students and factors associated with binge drinking. Method: The Harvard School of Public Health College Alcohol Study conducted a self-administered survey. The participants include a random sample of 7061 students aged Results: Underage students drink less often but have more drinks per occasion, are more likely to drink in private settings (off-campus, dormitory, and fraternity parties), and pay less per drink than do of-age students. Correlates of underage binge drinking include residence in a fraternity or sorority (odds ratio [OR]=6.2), very easy access to alcohol (OR=3.3), obtaining drinks at lower prices (OR=2.1, for under $1 each or a set fee for unlimited drinks), and drinking beer (OR=9.5). Conclusions: Effective controls on price, access, and fraternity and off-campus parties, and reinforcing minimum drinking age laws are needed to reduce the high levels of binge drinking and related health and behavioral problems of underage students.
Are We Doing Our Homework

[Clin Psychol Sci Prac 17: 162–165, 2010] Kazantzis, Whittington, and Dattilio (2010) provide convincing evidence for the role of homework in effective psychotherapy. Homework is a common element of many empirically supported treatments for a variety of clinical problems, but we cannot assume that homework is used commonly in routine, community-based psychotherapy. This commentary reviews emerging data on the use of homework in routine care for childhood behavioral disorders, and identifies potential barriers of use as well as research priorities to address these barriers.
Serotonin syndrome as a consequence of drug-resistant infections: an interaction between linezolid and citalopram.

This patient is an 85-year-old woman with a history of hypertension, depression, essential tremor, and pulmonary embolus. She was admitted to our nursing home after a prolonged hospitalization. She was treated there for a systemic infection with oxacillin-resistant Staphylococcus aureus originating from a tibial bursitis. The patient refused intravenous vancomycin and was started on oral linezolid. She was also continued on citalopram because of continued evidence of depression. Shortly after starting linezolid, the patient was transferred to our skilled nursing facility for rehabilitation. After transfer, the patient showed a significant decline in her overall status compared with baseline. Her benign tremor became significantly worse to the point of interfering with ambulation and feeding. She also became very confused and restless. No obvious source of infection was identified for these changes. Thyroid-stimulating hormone was checked and was found to be normal. Psychiatry was involved as a result of worsening tremor, confusion, agitation, and restlessness. A drug–drug interaction was high on the differential diagnosis. On examination, the patient was found to have dysarthric speech. On neurologic examination, she exhibited resting tremor of the head, neck, and upper extremities along with some hyperreflexia and paratonia. Gait was impaired secondary to compensation for her tremors. She appeared confused and restless. She was observed weeping because of discouragement but expressed no suicidal ideation. Her Mini Mental Status Examination score was 27/30 with points off for recall.
Effect of Gamma Radiation on the Insecticidal Efficiency of Malathion Deposits on Wheat and Kraft Paper

Malathion deposits on wheat and Kraft paper were irradiated with several dosage levels of gamma radiation. After various periods of aging, samples were bioassay with adults of the red flour beetle, Tribolium castaneum (Herbst). Chemical analyses were done concurrently with the bioassays. Neither the toxicity nor the chemical degradation of the malathion deposits was significantly affected by irradiation with gamma dosages ranging from intermediate (25 krad) to massive (4.3 mrad).
Force and pressure study of thick cambered/twisted 58 deg delta wings

This paper summarizes the results of a force, moment, and pressure experiment involving six thick, cambered and twisted, delta wings with 58 deg leading-edge sweep. The goal of this study was to determine a configuration that was self-trimming at a design lift coefficient of 0.25 and Mach number of 0.80. Analysis of the forces and moments data did not indicate a configuration that satisfied the design constraints; however, the configuration that came closest and had the best overall performance was selected for detailed pressure study. Wing pressure data and limited surface oil-flow photographs for this configuration showed attached flow at the design point. The associated inviscid forces and moments solutions from the Vortex Lattice Method-Suction Analogy, PAN AIR, FLO-28, and FLO-57 were compared with experimental data and showed varying levels of success. The computed pressures obtained from the PAN AIR, FLO-28, and FLO-57 for an angle of attack of 6.08 deg underpredicted the measured upper-surface pressure peak. At higher angles of attack, the lower-surface theoretical and measured pressure data compared well, whereas the upper-surface pressure predictions only approximated the trends but not the pressure level. Of the three codes, the PAN AIR is shown to be the best overall predictor.
A characterization of the multivariate normal distribution

Abstract Let X j ( j = 1,…, n ) be i.i.d. random variables, and let Y ′ = ( Y 1 ,…, Y m ) and X ′ = ( X 1 ,…, X n ) be independently distributed, and A = ( a jk ) be an n × n random coefficient matrix with a jk = a jk ( Y ) for j , k = 1,…, n . Consider the equation U = AX , Kingman and Graybill [ Ann. Math. Statist. 41 (1970)] have shown U ∼ N ( O,I ) if and only if X ∼ N ( O,I ). provided that certain conditions defined in terms of the a jk are satisfied. The task of this paper is to delete the identical assumption on X 1 ,…, X n and then generalize the results to the vector case. Furthermore, the condition of independence on the random components within each vector is relaxed, and also the question raised by the above authors is answered.
CONSISTENT SPECIFICATION TESTING FOR CONDITIONAL SYMMETRY

This paper presents a consistent specification test of conditional symmetry using a kernel method. The test statistic is shown to be asymptotically distributed as standard normal under the null hypothesis of conditional symmetry and consistent against any conditional asymmetric distribution. Power against local alternatives is also investigated. A Monte Carlo simulation is provided to evaluate the finite-sample performance of the test.
Monte carlo simulations of protein folding. II. Application to protein A, ROP, and crambin

The hierarchy of lattice Monte Carlo models described in the accompanying paper (Kolinski, A., Skolnick, J. Monte Carlo simulations of protein folding. I. Lattice model and interaction scheme. Proteins 18:338-352, 1994) is applied to the simulation of protein folding and the prediction of 3-dimensional structure. Using sequence information alone, three proteins have been successfully folded: the B domain of staphylococcal protein A, a 120 residue, monomeric version of ROP dimer, and crambin. Starting from a random expanded conformation, the model proteins fold along relatively well-defined folding pathways. These involve a collection of early intermediates, which are followed by the final (and rate-determining) transition from compact intermediates closely resembling the molten globule state to the native-like state. The predicted structures are rather unique, with native-like packing of the side chains. The accuracy of the predicted native conformations is better than those obtained in previous folding simulations. The best (but by no means atypical) folds of protein A have a coordinate rms of 2.25 A from the native C alpha trace, and the best coordinate rms from crambin is 3.18 A. For ROP monomer, the lowest coordinate rms from equivalent C alpha s of ROP dimer is 3.65 A. Thus, for two simple helical proteins and a small alpha/beta protein, the ability to predict protein structure from sequence has been demonstrated.
Mouse myelomas and lymphomas in culture

Abstract The establishment and growth characteristics of nine different long-term continuous in vitro cell lines originating from five myeloma anad two lymphoma tumor lines of independent origin in BALB/c or C3H mice, is described. Different culture lines were initiated by somewhat different methods but they now all grow in fortified Eagle's medium supplemented with horse serum as stationary suspension cultures with doubling times between 16 and 26 h. Some of the lines are near tetraploid and contain marker chromosomes while others are diploid or near-diploid. Several of the myeloma lines synthesize and secrete immunoglobulin while others, probably as a consequence of mutation, do not. The two lymphoma lines may synthesize Ig but do not appear to secrete it. Assays of the growth inhibiting activities of thymidine and hydrocortisone show that the lymphoma cells are much more sensitive than the myeloma cells. There is evidence suggesting that in these respects, as well as others, the cultured cell lines possess some of the differentiated characteristics of normal lymphocytes and plasma cells. Possible uses of the cell lines to analyze at the biochemical level the modes of genetic control of such characteristics are discussed.
On the Algorithmic Aspects of Discrete and Lexicographic Helly-Type Theorems and the Discrete LP-Type Model

Helly's theorem says that, if every $d+1$ elements of a given finite set of convex objects in $\mathbb{R}^d$ have a common point, there is a point common to all of the objects in the set. In discrete Helly theorems the common point should belong to an a priori given set. In lexicographic Helly theorems the common point should not be lexicographically greater than a given point. Using discrete and lexicographic Helly theorems we get linear time solutions for various optimization problems. For this, we introduce the DLP-type (discrete linear programming-type) model, and provide new algorithms that solve in randomized linear time fixed-dimensional DLP-type problems. For variable-dimensional DLP-type problems, our algorithms run in time subexponential in the combinatorial dimension. Finally, we use our results in order to solve in randomized linear time problems such as the discrete $p$-center on the real line, the discrete weighted 1-center problem in $\mathbb{R}^d$ with either $l_1$ or $l_\infty$ norm, the standard (continuous) problem of finding a line transversal for a totally separable set of planar convex objects, a discrete version of the problem of finding a line transversal for a set of axis-parallel planar rectangles, and the (planar) lexicographic rectilinear $p$-center problem for $p=1,2,3$. These are the first known linear time algorithms for these problems. Moreover, we use our algorithms to solve in randomized subexponential time various problems in game theory, improving upon the best known algorithms for these problems.
Morphology of the introvert and associated structures of the priapulid Tubiluchus corallicola from Bermuda

A scanning electron microscopical (SEM) and light micro-anatomical study of the anterior abdomen, tubuli, introvert, scalids, buccal tube, peribuccal nerve ring and pharynx of adult Tubiluchus corallicola Land, 1968 (phylum Priapulida) is presented. Where possible, these findings are compared with those of other priapulids and their potential functional properties are discussed. The presence of 25 longitudinal rows of introvert scalids is proposed as one of the few stable external characters of priapulids. The musculature of the bilaterally symmetrical introvert is shown to be of the basic priapulid type. The pectinate teeth of the anterior pharynx are considered to function as detrital rakes or sieves, and the unique pharyngeal musculature of T. corallicola is shown to be a specialized type which could be derived from the more general priapulid form.
Su1463 Endoscopic Therapy With Transpapillary Stenting Is Effective in Patients With Grade B Pancreatic Distal Occlusion Failure After Distal Pancreatectomy or Splenectomy

Endoscopic Therapy With Transpapillary Stenting Is Effective in Patients With Grade B Pancreatic Distal Occlusion Failure After Distal Pancreatectomy or Splenectomy Kavous Pakseresht, Savio Reddymasu, Brian Moloney, Daniel C. Buckles, Melissa M. Oropeza-Vail, Scott Stanley, Tuba Esfandyari, Scott Grisolano, Mojtaba S. Olyaee Kansas university Medical Center, Kansas city, KS Purpose: To study the efficacy of endoscopic retrograde cholangiopancreatography (ERCP) with transpapillary pancreatic duct (PD) stent placement in a series of patients with grade B (International Study Group For Pancreatic Fistula classification) pancreatic distal occlusion failure (DOF) occurring as a complication of distal pancreatectomy (DP) or splenectomy. Methods: Eight (5 female) patients with a mean age of 55 years (range: 22-69) underwent endotherapy for grade B pancreatic DOF between January 1999 and May 2010. Base line demographics, duration between surgery and initial ERCP, pancreatogram findings, and details regarding endotherapy during the ERCP were reviewed. Results: The pancreatic DOF was a complication of DP in 6, DP with splenectomy in 1, and splenectomy alone in 1 patient. The mean amylase and lipase level in the peritoneal fluid at the time of initial ERCP was 15648 u/L and 2897 u/L respectively. The mean duration between surgery and the initial ERCP was 47 days (range: 13-85 days). The PD was cannulated and a transpapillary PD stent was deployed successfully in all patients. Seven patients (87%) had complete resolution of the pancreatic DOF after a mean number of 4 ERCP’s (range: 2-10). The mean duration between the initial PD stent placement and resolution of the pancreatic DOF was 121 days (range: 44-389 days). One patient was lost to follow-up. No serious complications were reported during ERCP. Conclusion: 1) A majority of grade B pancreatic DOF’s occurring as a complication of DP or splenectomy resolve after transpapillary PD stent placement. However, patients might require several endoscopic procedures prior to complete resolution of the pancreatic DOF. 2) ERCP with PD stent placement should be offered to patients with pancreatic DOF as a safe and effective alternative to surgery.
Case report 790

We have presented a case of mesenchymal chondrosarcoma of the sacrum in a 20-year-old woman. We have emphasized that CT and MR are the best imaging modalities for the assessment of the neoplasm and the surgical decision-making process. Histologic study is mandatory for diagnosis of the tumor, with its characteristic dual pattern of chondroid tissue and undifferentiated mesenchymal cells. The rarity of the neoplasm, its deep pelvic location and nonspecific symptomatology led to a late diagnosis and an unfortunate but necessary sacral amputation resulting in bladder and bowel functional sequelae.