cssi/SciSciGPT-SciSciNet huggingface dataset:
The anti-motility signaling mechanism of TGFβ3 that controls cell traffic during skin wound healing.

When skin is wounded, migration of epidermal keratinocytes at the wound edge initiates within hours, whereas migration of dermal fibroblasts toward the wounded area remains undetectable until several days later. This “cell type traffic” regulation ensures proper healing of the wound, as disruptions of the regulation could either cause delay of wound healing or result in hypertrophic scars. TGFβ3 is the critical traffic controller that selectively halts migration of the dermal, but not epidermal, cells to ensure completion of wound re-epithelialization prior to wound remodeling. However, the mechanism of TGFβ3's anti-motility signaling has never been investigated. We report here that activated TβRII transmits the anti-motility signal of TGFβ3 in full to TβRI, since expression of the constitutively activated TβRI-TD mutant was sufficient to replace TGFβ3 to block PDGF-bb-induced dermal fibroblast migration. Second, the three components of R-Smad complex are all required. Individual downregulation of Smad2, Smad3 or Smad4 prevented TGFβ3 from inhibiting dermal fibroblast migration. Third, Protein Kinase Array allowed us to identify the protein kinase A (PKA) as a specific downstream effector of R-Smads in dermal fibroblasts. Activation of PKA alone blocked PDGF-bb-induced dermal fibroblast migration, just like TGFβ3. Downregulation of PKA's catalytic subunit nullified the anti-motility signaling of TGFβ3. This is the first report on anti-motility signaling mechanism by TGFβ family cytokines. Significance of this finding is not only limited to wound healing but also to other human disorders, such as heart attack and cancer, where the diseased cells have often managed to avoid the anti-motility effect of TGFβ.
Development Administration and American Foreign Assistance Policy: An Assessment of Theory and Practice in Aid

ABSTRACT A variety of interventions to improve development management have been tried by the U.S. Agency for International Development over the past 30 years. These have included technology transfer and management control approaches as well as ‘“learning process”’ and local capacity building methods. All of these interventions have been found to have advantages and limitations under different conditions. This article reviews experience with development administration theories and practices in U.S. foreign aid programs and examines the results. The lessons of experience indicate that no one approach to development administration is universally applicable in foreign assistance programs in developing countries but that some approaches are more applicable to capital development than to human resources development projects.
Hydrotreated Renewable Jet Fuel Ignition Delay Performance in a Military Diesel Engine: An Experimental and Modeling Study

In an effort towards predicting the combustion behavior of a new fuel in a conventional diesel engine, Hydrotreated Renewable Jet (HRJ) fuel was first run in a military diesel engine across the entire speed-load operating range. Ignition delay was characterized for this fuel at each operating condition. Next, a HRJ surrogate fuel was developed in order to predict the combustion performance of this new renewable fuel. A chemical ignition delay was then predicted across the speed-load range using a detailed chemical kinetic mechanism model based on an 8-component surrogate representative of HRJ. The modeling suggests that rich fuel-air parcels developed from the diesel spray are the first to ignite. The chemical ignition delay results also show decreasing ignition delays with increasing engine load and speed just as shown by the empirical data. A moderate difference between the total and chemical ignition delays was then characterized as a physical delay period which scales inversely with engine speed. The approach used in this study suggests that the ignition delay and thus start of combustion may be predicted with reasonable accuracy allowing for the analytical assessment of the acceptability of a new fuel in a conventional engine.Copyright © 2012 by ASME
Effect of sodium arsenite on heme metabolism in cultured chick embryo hepatocytes.

We had previously reported that low concentrations of sodium arsenite (1-5 microM) decreased the induction of cytochrome P450 CYP1A and CYP2H in cultured chick embryo hepatocytes in parallel with increases in heme oxygenase. However, in those studies exogenous heme did not prevent the decrease in CYPs. In this study, we investigated the effect of arsenite on the synthesis and degradation of heme. Arsenite had no effect on induction of 5-aminolevulinic acid synthase mRNA or activity. Arsenite, at concentrations from 1 to 25 microM, had no effect on protoporphyrin synthesis from 5-aminolevulinic acid and did not increase the accumulation of other porphyrins, indicating that the enzymes in the pathway between 5-aminolevulinic acid synthase and ferrochelatase were unaffected by arsenite. Synthesis of heme from radioactive 5-aminolevulinic acid was slightly decreased (less than 20%) by 2.5 microM arsenite, a concentration that decreased induction of CYP1A and CYP2H by greater than 50%. Rates of biliverdin formation and degradation of exogenous heme were not different in cultures treated simultaneously with arsenite and heme or with heme alone. However, arsenite treatment increased biliverdin formation from heme synthesized from added 5-aminolevulinic acid by 60% and decreased the endogenous heme content of the cells by 30%. Our results suggest that although 2.5 microM arsenite induced heme oxygenase four- to sixfold, this had no effect on degradation of exogenous heme. Degradation of heme synthesized from 5-aminolevulinic acid was increased but this did not affect the regulatory heme pool.
Schedules for a two-stage hybrid flowshop with parallel machines at the second stage

Approximate solution algorithms are developed to find a minimum makespan schedule in a two-stage hybrid flowshop when the second stage consists of multiple identical machines. Computational experience comparing the ‘approximate’ makespans with their respective global lower bounds for large problems indicates that proposed polynomially bounded approximate algorithms are quite effective. It is shown that the proposed heuristic algorithms can be used to improve the efficiency of an existing branch and bound algorithm.
Growth of Red Drum Larvae in the Laboratory

Abstract Larval red drums Sciaenops ocellatus were raised at 24 C and 28 C in the laboratory. Length of newly hatched larvae increased steadily but their mean dry weights first decreased over the first 5 days (until yolk was fully resorbed), then increased rapidly. At day 15, there were significant differences in mean standard lengths between larvae grown at the two temperatures (4.8 mm at 24 C; 5.1 mm at 28 C; P < 0.05); larvae grown at the higher temperature were also markedly heavier (363.7 μg versus 186.3 μg; P < 0.01). Transition from the yolk-sac stage to active feeding occurred at day 5–6 when larvae were about 2.7 mm long.
Postictal serum levels of antiepileptic drugs for detection of noncompliance

Medication noncompliance (NC) is thought to be a major cause of insufficient seizure control. In an explorative study we investigated whether postictal serum levels (PISLs) of antiepileptic drugs (AEDs) are a reliable indicator of NC. Young adults with epilepsy on a stable AED regimen were asked to contact medical service as soon as possible when a seizure occurred to obtain serum levels of their AEDs. PISLs were compared with the mean value of two routine serum levels of the same medication. PISLs lower than 50% of the individual reference value were regarded as an indicator of NC. PISL samples in 61 seizures of 52 patients treated mainly with carbamazepine, valproic acid, or lamotrigine were evaluated. A drop in serum levels >50% indicating NC was noted in 44.3% of the seizures. Determination of PISLs seems to be a simple and useful method for detecting or ruling out irregular intake patterns as a cause of "breakthrough" seizures.
Two distinct processes are evident in rat cone flicker ERG responses at low and high temporal frequencies

The electroretinogram (ERG) provides a noninvasive, objective measure of retinal function, and is one of the most widely used diagnostic tools in the study of visual disorders. Although rodents are often used in the study of retinal disease, the properties of the flicker ERG of the rodent retina have not been fully characterized. Here, we show that the fundamental response of the rat ERG to sine-wave flicker exhibited a low-pass pattern in the frequency range from 2 to 30 Hz, whereas the second harmonic (F2) showed a more complex frequency-response relation. The F2 component represented only a small fraction of the ERG response at low temporal frequencies (below 12 Hz), but it made a substantial contribution to responses at high frequencies. The contrast-response relation was linear when tested with a low-frequency (6 Hz) stimulus, but saturated in response to a high-frequency (20 Hz) stimulus. After intravitreal injection of L-AP4, a specific blocker of the retinal ON pathway, the flicker responses elicited by either 6- or 20-Hz stimuli were greatly reduced in amplitude, whereas only a very slight enhancement was seen after the application of PDA, a drug that blocks retinal OFF-pathway activity. Based on the observed differences in the degree of nonlinearity, and contrast-response properties of the rat flicker ERG at low and high frequencies, as well as the pharmacological results, we postulate that sustained and transient ON bipolar cells generate the flicker ERG responses elicited at low and high temporal frequencies, respectively.
The JK Method: A Procedure for Finding the Eigenvectors and Eigenvalues of a Real Symmetric Matrix

A procedure for finding the eigenvectors and eigenvalues of a real symmetric matrix, dubbed the 'JK method,' is presented. It is similar to Jacobi's classic procedure, but involves only a postmultiplying orthonormal transformation. When both eigenvectors and eigenvalues are wanted, the JK method has advantages, both in computational time and in storage requirements, over Jacobi's procedure. (Received September 1971)
Statistical analysis of survival data from radiation countermeasure experiments.

We present an introduction to, and examples of, Cox proportional hazards regression in the context of animal lethality studies of potential radioprotective agents. This established method is seldom used to analyze survival data collected in such studies, but is appropriate in many instances. Presenting a hypothetical radiation study that examines the efficacy of a potential radioprotectant both in the absence and presence of a potential modifier, we detail how to implement and interpret results from a Cox proportional hazards regression analysis used to analyze the survival data, and we provide relevant SAS® code. Cox proportional hazards regression analysis of survival data from lethal radiation experiments (1) considers the whole distribution of survival times rather than simply the commonly used proportions of animals that survived, (2) provides a unified analysis when multiple factors are present, and (3) can increase statistical power by combining information across different levels of a factor. Cox ...
Detection of frequency modulation (FM) in the presence of a second FM tone.

A series of three experiments was undertaken to investigate detection of sinusoidal frequency modulation (FM) in the presence of FM at a separate frequency. The first experiment measured detection of modulation for an FM tone with a modulation frequency ( f m) of 6 Hz as a function of carrier frequency ( f c) under three conditions: (1) in quiet, (2) in the presence of a 2500‐Hz pure tone, and (3) in the presence of a 2500‐Hz FM tone with f m=6 Hz, modulating in phase with the signal. Detection of FM in the presence of the second FM tone was worse than for either the signal presented in quiet or in the presence of the unmodulated tone. Threshold varied as an inverse function of frequency separation between the signal and the masker. In the second experiment, FM detection for a signal with f c=1900 Hz and f m=6 Hz was measured as a function of the modulation frequency ( f m=2–18 Hz) of the 2500‐Hz masker tone. FM detection improved significantly with increasing difference between the modulation frequencies...
Children's Beliefs About Drinking

This paper reports the results of a study in which age (grade level), racial/ethnic, and gender differences in beliefs and perceived norms about drinking were examined in a multi-ethnic urban sample of 4th through 7th grade children. Results showed that older children held beliefs and perceived norms that were more favorable toward drinking than younger children. The major difference between older and younger children lay in their differential estimates of the likelihood of certain consequences occurring and not in their evaluation of these consequences of drinking. Further, older children not only displayed less motivation to comply with their parents and greater motivation to comply with their peers, but they also perceived their parents, as well as their peers, as less disapproving of drinking than did younger children. There were few gender or race/ethnicity differences at these ages in children's beliefs and perceived norms about drinking.
The role of misconceptions on Latino women's acceptance of emergency contraceptive pills

The goal of this study was to assess factors associated with hormonal emergency contraception (EC) awareness and acceptability among a sample of low-income Latino women receiving care in two university reproductive health clinics. A total of 297 Latino women, 18-43 years of age, completed a survey about EC awareness during a clinic visit, between January and May 2003. Those women with some degree of awareness (n = 73) also completed questions related to their acceptance of EC. Factors examined included language preference, demographic characteristics, pregnancy history, contraceptive use history and knowledge and concerns about EC usage. We found that only 41% of English-speaking and 17% of Spanish-speaking women had ever heard of EC [adjusted odds ratio (OR) = 2.9, confidence interval (CI): 1.3, 6.4]. Among those aware of EC, unwillingness to use this method was associated with low levels of knowledge about the EC mechanism of action, but not about the EC regimen (adjusted OR = 0.5; CI: 0.3, 0.9). Specific misconceptions underlying their objections included the belief that women are more likely to get pregnant in the beginning of their cycle compared to the middle (unadjusted OR = 6.3; CI: 1.8, 22.6), and a belief that EC prevents implantation rather than ovulation (unadjusted OR = 5.7; CI: 1.2, 28.1). The extent to which the women considered EC to be morally wrong depended on their misconceptions about the EC mechanism, not on their religious background. The link between expressed moral concerns and incorrect knowledge coupled with its lack of association to religiosity suggests that enhanced education can help to alleviate moral objections, thereby increasing potential usage of EC to prevent unintended pregnancy.
Motif analysis of amphioxus, lamprey and invertebrate estrogen receptors: Toward a better understanding of estrogen receptor evolution

The origins of steroid-dependent regulation of the vertebrate estrogen receptor (ER) are poorly understood. We used artificial-intelligence-based software to construct 12 motifs specific to the estrogen-binding domain of ERα and ERβ in land vertebrates and teleosts. We mapped these ER-specific motifs onto the sequences of lamprey, amphioxus, and invertebrate and selected vertebrate ERs and estrogen-related receptors (ERR). Lamprey ER contains 11 motifs common to ERs in the training set. In contrast, amphioxus ER contains only six motifs. Unexpectedly, human and amphioxus ERRs contain nine motifs. We mapped the 12 motifs onto an alignment of human, lamprey and amphioxus ERs, which depicted residues in human ERα that interact with estradiol, which revealed significant differences between amphioxus ER and vertebrate ERs in the steroid-binding domain. This suggests unusual ligand recognition in amphioxus ER.
Coupling of Energy Metabolism and Synaptic Transmission at the Transcriptional Level: Role of Nuclear Respiratory Factor 1 in Regulating both Cytochrome c Oxidase and NMDA Glutamate Receptor Subunit Genes

Neuronal activity and energy metabolism are tightly coupled processes. Regions high in neuronal activity, especially of the glutamatergic type, have high levels of cytochrome c oxidase (COX). Perturbations in neuronal activity affect the expressions of COX and glutamatergic NMDA receptor subunit 1 (NR1). The present study sought to test our hypothesis that the coupling extends to the transcriptional level, whereby NR1 and possibly other NR subunits and COX are coregulated by the same transcription factor, nuclear respiratory factor 1 (NRF-1), which regulates all COX subunit genes. By means of multiple approaches, including in silico analysis, electrophoretic mobility shift and supershift assays, in vivo chromatin immunoprecipitation, promoter mutations, and real-time quantitative PCR, NRF-1 was found to functionally bind to the promoters of Grin 1 (NR1), Grin 2b (NR2b) and COX subunit genes, but not of Grin2a and Grin3a genes. These transcripts were upregulated by KCl and downregulated by tetrodotoxin (TTX) in cultured primary neurons. However, silencing of NRF-1 with small interference RNA blocked the upregulation of Grin1, Grin2b, and COX induced by KCl, and overexpression of NRF-1 rescued these transcripts that were suppressed by TTX. NRF-1 binding sites on Grin1 and Grin2b genes are also highly conserved among mice, rats, and humans. Thus, NRF-1 is an essential transcription factor critical in the coregulation of NR1, NR2b, and COX, and coupling exists at the transcriptional level to ensure coordinated expressions of proteins important for synaptic transmission and energy metabolism.
Stabilization of Uncertain Systems using State Detection and Application to a Maglev Vehicle

We present a deterministic treatment for the control of a class of systems with time-varying uncertainties. For the controller design we assume only that the uncertainties lie within some prescribed sets. The controller, based on the knowledge of the present state, guarantees desired performance of the system, including uniform boundedness and uniform ultimate boundedness. If, however, only output instead of state is available for the controller, the construction of a suitable observer is introduced. The resulting plant-observer-controller system is shown to preserve the above-mentioned desired performance. The theoretical result is illustrated by an application to the suspension control of a Maglev (magnetically levitated) Vehicle.
Lewis-acid catalysis of the asymmetric Diels-Alder reaction of dimenthyl fumarate and cyclopentadiene

Abstract The effects of Lewis acid catalysts, solvent, catalyst loading, and temperature on the diastereomeric excess in the Diels–Alder reaction of (+)-dimenthyl fumarate and cyclopentadiene were investigated.
Polychlorinated Biphenyls (Aroclor 1254) Do Not Uniformly Produce Agonist Actions on Thyroid Hormone Responses in the Developing Rat Brain

Thyroid hormone (TH) is essential for normal brain development, and polychlorinated biphenyls (PCBs) are known to interfere with TH action in the developing brain. Thus, it is possible that the observed neurotoxic effects of PCB exposure in experimental animals and humans are mediated in part by their ability to interfere with TH signaling. PCBs may interfere with TH signaling by reducing circulating levels of TH, acting as TH receptor analogs, or both. If PCBs act primarily by reducing serum TH levels, then their effects should mimic those of low TH. In contrast, if PCBs act primarily as TH agonists in the developing brain, then they should mimic the effect of T4 in hypothyroid animals. We used a two-factor design to test these predictions. Both hypothyroidism (Htx) and/or PCB treatment reduced serum free and total T4 on postnatal d 15. However, only Htx increased pituitary TSHβ expression. RC3/neurogranin expression was decreased by Htx and increased by PCB treatment. In contrast, Purkinje cell protein-2 expression was reduced in hypothyroid animals and restored by PCB treatment. Finally, PCB treatment partially ameliorated the effect of Htx on the thickness of the external granule layer of the cerebellum. These studies demonstrate clearly that PCB exposure does not mimic the effect of low TH on several important TH-sensitive measures in the developing brain. However, neither did PCBs mimic T4 in hypothyroid animals on all end points measured. Thus, PCBs exert a complex action on TH signaling in the developing brain.
A novel superfamily containing the β-grasp fold involved in binding diverse soluble ligands

Background: Domains containing the β-grasp fold are utilized in a great diversity of physiological functions but their role, if any, in soluble or small molecule ligand recognition is poorly studied. Results: Using sensitive sequence and structure similarity searches we identify a novel superfamily containing the β-grasp fold. They are found in a diverse set of proteins that include the animal vitamin B12 uptake proteins transcobalamin and intrinsic factor, the bacterial polysaccharide export proteins, the competence DNA receptor ComEA, the cob(I)alamin generating enzyme PduS and the Nqo1 subunit of the respiratory electron transport chain. We present evidence that members of this superfamily are likely to bind a range of soluble ligands, including B12. There are two major clades within this superfamily, namely the transcobalamin-like clade and the Nqo1-like clade. The former clade is typified by an insert of a β-hairpin after the helix of the β-grasp fold, whereas the latter clade is characterized by an insert between strands 4 and 5 of the core fold. Conclusion: Members of both clades within this superfamily are predicted to interact with ligands in a similar spatial location, with their specific inserts playing a role in the process. Both clades are widely represented in bacteria suggesting that this superfamily was derived early in bacterial evolution. The animal lineage appears to have acquired the transcobalamin-like proteins from low GC Gram-positive bacteria, and this might be correlated with the emergence of the ability to utilize B12 produced by gut bacteria. Reviewers: This article was reviewed by Andrei Osterman, Igor Zhulin, and Arcady Mushegian.
Analysis of Gene Targeting and Intrachromosomal Homologous Recombination Stimulated by Genomic Double-Strand Breaks in Mouse Embryonic Stem Cells

To investigate the effects of in vivo genomic DNA double-strand breaks on the efficiency and mechanisms of gene targeting in mouse embryonic stem cells, we have used a series of insertion and replacement vectors carrying two, one, or no genomic sites for the rare-cutting endonuclease I-SceI. These vectors were introduced into the hypoxanthine phosphoribosyltransferase (hprt) gene to produce substrates for gene-targeting (plasmid-to-chromosome) or intrachromosomal (direct repeat) homologous recombination. Recombination at the hprt locus is markedly increased following transfection with an I-SceI expression plasmid and a homologous donor plasmid (if needed). The frequency of gene targeting in clones with an I-SceI site attains a value of 1%, 5,000-fold higher than that in clones with no I-SceI site. The use of silent restriction site polymorphisms indicates that the frequencies with which donor plasmid sequences replace the target chromosomal sequences decrease with distance from the genomic break site. The frequency of intrachromosomal recombination reaches a value of 3.1%, 120-fold higher than background spontaneous recombination. Because palindromic insertions were used as polymorphic markers, a significant number of recombinants exhibit distinct genotypic sectoring among daughter cells from a single clone, suggesting the existence of heteroduplex DNA in the original recombination product.
Dehydroepiandrosterone (DHEA) increases territorial song and the size of an associated brain region in a male songbird.

Abstract In many species, male territorial aggression is tightly coupled with gonadal secretion of testosterone (T). In contrast, in song sparrows (Melospiza melodia morphna), males are highly aggressive during the breeding (spring) and nonbreeding (autumn and early winter) seasons, but not during molt (late summer). In aggressive nonbreeding song sparrows, plasma T levels are basal (≤0.10 ng/ml), and castration has no effect on aggression. However, aromatase inhibitors reduce nonbreeding aggression, indicating a role for estrogen in wintering males. In the nonbreeding season, the substrate for brain aromatase is unclear, because plasma T and androstenedione levels are basal. Aromatizable androgen may be derived from plasma dehydroepiandrosterone (DHEA), an androgen precursor. DHEA circulates at elevated levels in wintering males (∼0.8 ng/ml) and might be locally converted to T in the brain. Moreover, plasma DHEA is reduced during molt, as is aggression. Here, we experimentally increased DHEA in wild nonbreeding male song sparrows and examined territorial behaviors (e.g., singing) and discrete neural regions controlling the production of song. A physiological dose of DHEA for 15 days increased singing in response to simulated territorial intrusions. In addition, DHEA treatment increased the volume of a telencephalic brain region (the HVc) controlling song, indicating that DHEA can have large-scale neuroanatomical effects in adult animals. The DHEA treatment also caused a slight increase in plasma T. Exogenous DHEA may have been metabolized to sex steroids within the brain to exert these behavioral and neural effects, and it is also possible that peripheral metabolism contributed to these effects. These are the first results to suggest that exogenous DHEA increases male–male aggression and the size of an entire brain region in adults. The data are consistent with the hypothesis that DHEA regulates territorial behavior, especially in the nonbreeding season, when plasma T is basal.
Identification of genes whose expression is altered during mitosuppression in livers of ethinyl estradiol-treated female rats

In this study, our goal was to identify genes whose expression in liver is altered in female F-344 rats during mitosuppression induced by 42 days of ethinyl estradiol (EE) treatment (Yager et al., Carcinogenesis, 15, 2117-2123, 1994). Northern analysis demonstrated that the mRNA levels for transforming growth factor-beta1 (TGF-beta1) and the mannose 6-phosphate/insulin-like growth factor II receptor were significantly increased by EE treatment. Ten cDNA clones representing mRNAs whose expression was increased two- to four-fold in the mitosuppressed livers were identified by differential display. Sequence analysis revealed that one was homologous to the S-24 ribosomal protein and another to mitochondrial ATPase subunit e. The remaining clones showed no homology to known genes in GenBank. However, the expression of clones 15, 16 and 17 was increased in HepG2 cells following treatment with doxorubicin suggesting their induction by oxidative DNA damage. These results suggest that two independent but interrelated signalling pathways, one mediated through transforming growth factor-beta and the other through oxidative DNA damage, may contribute to hepatic mitosuppression caused by EE, perhaps through activation of cyclin-dependent kinase inhibitors.
Defects in CdTe single crystals grown by very fast vapor growth technique

Single crystals of CdTe were obtained by the very fast vapor growth technique. The four major defects, namely, multi-grains, lamellar, lateral, and micro-twins, which are fatal to the single crystallinity, were eliminated or limited by increasing the growth stability. This investigation indicates that the latent heat under high growth rate conditions should not be neglected. A model is developed to explain the effects of latent heat on the growth stability at the interface. A relationship between crystal morphologies and growth conditions was established. It strongly suggests that the above defects are growth stability related. The origin of twinning, dominated by growth stability, is discussed in this paper.
BRAIN CHOLINESTERASE ACTIVITY OF APPARENTLY NORMAL WILD BIRDS

Organophosphorous and carbamate pesticides are potent anticholinesterase substances that have killed large numbers of wild birds of various species. Cause of death is diagnosed by demonstration of depressed brain cholinesterase (ChE) activity in combination with chemical detection of anticholinesterase residue in the affected specimen. ChE depression is determined by comparison of the affected specimen to normal ChE activity for a sample of control specimens of the same species, but timely procurement of controls is not always possible. Therefore, a reference file of normal whole brain ChE activity is provided for 48 species of wild birds from North America representing 11 orders and 23 families for use as emergency substitutes in diagnosis of anticholinesterase poisoning. The ChE values, based on 83 sets of wild control specimens from across the United States, are reproducible provided the described procedures are duplicated. Overall, whole brain ChE activity varied nearly three-fold among the 48 species...
A case of emphysematous hepatitis with spontaneous pneumoperitoneum in a patient with hilar cholangiocarcinoma

An 80-year-old woman with hilar cholangiocarcinoma was hospitalized due to sudden-onset abdominal pain. Computed tomography revealed hepatic necrosis accompanied with emphysematous change in the superior segment of the right liver (S7/S8), implying spontaneous rupture, based on the presence of perihepatic free air. Although urgent percutaneous drainage was performed, neither pus nor fluids were drained. These findings suggest emphysematous hepatitis with a hepatic mass. Despite the application of intensive care, the patient's condition deteriorated rapidly, and she died 3 days after admission to hospital. Liver gas has been reported in some clinical diseases (e.g., liver abscess) to be caused by gas-forming organisms; however, emphysematous hepatitis simulating emphysematous pyelonephritis is very rare. The case reported here was of fatal emphysematous hepatitis in a patient with hilar cholangiocarcinoma.
Endoscopic craniofacial resection for pediatric esthesioneuroblastoma

Abstract Pediatric esthesioneuroblastomas are rare tumors with only about 100 reported cases. Standard surgical treatment is the open craniofacial resection. Pre-treatment imaging is critical for planning, and surgery remains the primary treatment modality followed by adjuvant therapy. We report a 12-year-old female who underwent endoscopic-assisted resection of an extensively calcified Kadish C and Dulguerov–Calcaterra T3N0M0 esthesioneuroblastoma. Following radiation therapy, she is disease-free 18 months post-treatment. Although there are few reported cases in pediatric patients, our case supports the use of endoscopic endonasal approaches for select pediatric skull base malignancies.
Performance evaluation of a second generation small animal PET

We have completed construction of a second-generation, single-plane small-animal PET instrument based on LSO detectors. The second-generation design addresses some of the limitations in the first-generation. The purpose of the work reported here was to characterize the physical performance of this instrument. Physical measurements presented include measurements of spatial resolution, sensitivity, linearity, scatter fraction and count-rate performance as well as the imaging of extended objects.
Photoperiodic control of the timing of testicular regression in white-throated sparrows

Abstract Photosensitive white-throated sparrows were transferred from a nonstimulatory LD 8:16 light cycle to either a stimulatory LD 13:11 or LD 20:4 light cycle on Day O. While some birds remained on these stimulatory photoperiods throughout the study, other birds were transferred from LD 13:11 to LD 20:4 on Day 50, or from LD 20:4 to LD 13:11 on Day 30 after the testes had reached a width of about 4.0 mm. The testes of birds continuously exposed to LD 13:11 grew more slowly, reached a larger maximum size, and spontaneously regressed later than the testes of birds continuously maintained on LD 20:4. Spontaneous testicular regression occurred at the same time (between Days 90 and 120) in sparrows that were transferred from LD 13:11 to LD 20:4 on Day 50 as in sparrows that were maintained continuously on LD 13:11. Following testicular growth in sparrows that were exposed initially to LD 20:4, the transfer to a presumably photostimulatory LD 13:11 cycle induced rapid testicular regression suggesting that LD 13:11 was no longer being interpreted as a long day. These results suggest that in nature, the timing of spontaneous testicular regression which occurs after a prolonged exposure to photostimulatory long days may be (1) programmed by the LD cycle the bird is exposed to during gonadal growth, and/or (2) due to a decrease in day length after the summer solstice even though the length of the day is still longer than that which previously initiated testicular growth.
Enhancing Ultrasonic Bond Development

To enhance ultrasonic bond development, an improved understanding is needed of the processes taking place at the bond interface. This work is designed to determine the role that bonding parameters play in contributing to bond development. Of particular concern are the effects created by the machine variables, i.e., load, power, and time. Silicon was selected as a bond surface for aluminum wire bonds. The brittle nature of silicon provides a permanent record of the bonding history. With both the crystal orientation and defects of the silicon well characterized prior to bonding, features such as the location of residual bonding strains in the silicon were determined. The pattern of partially bonded material exposed by peeling underdeveloped bonds simulates a torus (or doughnut) with an unbonded central region. Features of aluminum wire bonds to aluminum, glass, beryllium, and silicon were compared to show that a common mechanism exists independent of the bond surface material. The ability to bond to silicon varies with wire composition. For example, both Al-0.5% Mg and pure Al wires bond readily, while Al-l% Si wire does not. Two modes of material flow characterize interfacial behavior. Ultrasonic energy promotes a material softening which, in conjunction with the applied load, results in a gross flow to expose fresh material for bonding. In the second stage of material flow, a wave form is propagated through the wire to the periphery of the wire-silicon interface. This wave form is observed as a periodic cutting action into the silicon perpendicular to the pulsing direction. A fine ball-like formation in the grooves of the wave region at the bond zone was a feature common to the different bonding surfaces. The wavelength and wave amplitude vary linearly with the applied power as does the tip-to-tip displacement of the wedge. The groove spacings are of the same magnitude as the wedge displacement. For constant power and time, increased load increases the size of the central bond region that does not experience the wave action. For constant load and power, the width of the wave affected periphery increases toward the center of the bond with time. The method of thermally induced stacking faults by steam oxidation was used to characterize the residual bond strains in the silicon. Faulting was found in the peripheral region of the bond where the wave action was operable. This faulting correlated to stresses generated in the pulsing direction and not to the directions of gross material flow. Reliable bonding depends upon a proper control of the gross and wave flow processes by optimizing material properties as well as machine variables. A model has been developed to qualitatively relate the influence of these variables and the manner in which a change in one parameter affects the response of the remaining variables.
InGaAs/GaAs planar doped barrier electron emitters

Summary form only given. Emission from planar doped barrier (PDB) hot electron generators is demonstrated. The PDB structure consists of a sequence of n/sup +/-i- delta (p/sup +/)-i-n/sup + /layers. The delta (p/sup +/) layer is fully depleted, giving rise to a triangular barrier. An applied bias V forward biases the n/sup +/-i- delta (p/sup +/) junction while reverse biasing the delta (p/sup +/)-i-n/sup + /junction, much like the distribution of emitter-collector bias applied to a bipolar transistor with a fully depleted base. The reverse bias potential accelerates the electrons injected over the semiconductor barrier to a maximum kinetic energy determined by the applied potential. The final kinetic energy is lowered by scattering processes. The intrinsic efficiency of emission is therefore determined by quasi-ballistic transport across the acceleration region of the diode. >
Distinct mutational behaviors differentiate short tandem repeats from microsatellites in the human genome.

A tandem repeat’s (TR) propensity to mutate increases with repeat number, and can become very pronounced beyond a critical boundary, transforming it into a microsatellite (MS). However, a clear understanding of the mutational behavior of different TR classes and motifs and related mechanisms is lacking, as is a consensus on the existence of a boundary separating short TRs (STRs) from MSs. This hinders our understanding of MSs’ mutational properties and their effective use as genetic markers. Using indel calls for 179 individuals from 1000 Genomes Pilot-1 Project, we determined polymorphism incidence for four major TR classes, and formalized its varying relationship with repeat number using segmented regression. We observed a biphasic regime with a transition from a faster to a slower exponential growth at 9, 5, 4, and 4 repeats for mono-, di-, tri-, and tetranucleotide TRs, respectively. We used an in vitro mutagenesis assay to evaluate the contribution of strand slippage errors to mutability. STRs and MSs differ in their absolute polymorphism levels, but more importantly in their rates of mutability growth. Although strand slippage is a major factor driving mononucleotide polymorphism incidence, dinucleotide polymorphism incidence is greater than that expected due to strand slippage alone, indicating that additional cellular factors might be driving dinucleotide mutability in the human genome. Leveraging on hundreds of human genomes, we present the first comprehensive, genome-wide analysis of TR mutational behavior, encompassing several motif sizes and compositions.
Both information and social cohesion determine collective decisions in animal groups

During consensus decision making, individuals in groups balance personal information (based on their own past experiences) with social information (based on the behavior of other individuals), allowing the group to reach a single collective choice. Previous studies of consensus decision making processes have focused on the informational aspects of behavioral choice, assuming that individuals make choices based solely on their likelihood of being beneficial (e.g., rewarded). However, decisions by both humans and nonhuman animals systematically violate such expectations. Furthermore, the typical experimental paradigm of assessing binary decisions, those between two mutually exclusive options, confounds two aspects common to most group decisions: minimizing uncertainty (through the use of personal and social information) and maintaining group cohesion (for example, to reduce predation risk). Here we experimentally disassociate cohesion-based decisions from information-based decisions using a three-choice paradigm and demonstrate that both factors are crucial to understanding the collective decision making of schooling fish. In addition, we demonstrate how multiple informational dimensions (here color and stripe orientation) are integrated within groups to achieve consensus, even though no individual is explicitly aware of, or has a unique preference for, the consensus option. Balancing of personal information and social cues by individuals in key frontal positions in the group is shown to be essential for such group-level capabilities. Our results demonstrate the importance of integrating informational with other social considerations when explaining the collective capabilities of group-living animals.
A Cross-National Comparison of Some Social Patterns of Urban Pedestrians

An observational study of urban pedestrians in six national groupings was undertaken to provide quantitative cross-cultural data on various social behaviors in a naturalistic setting. At 155 sampling points in shopping, park, and entertainment areas within major cities in 3 Moslem countries (Turkey, Iran, and Afghanistan), England, West Germany, Sweden, Italy, and the United States, E recorded grouping, interaction, and physical contact patterns of 21, 316 pedestrians by sex and five estimated age categories. In representative major findings, national tendency to form groups was in the order given above, there were few national differences in interaction frequency, and Italians, West Germans, and Englishmen contacted more than Americans, Swedes, and Moslems.
Understanding self‐sufficiency of welfare leavers in Illinois: Elaborating models with psychosocial factors

With an interest in supporting the existing social capacities of leavers of Temporary Assistance to Needy Families (TANF), this chapter incorporates psychosocial factors into a structural equation model that includes the outcomes of post-exit employment and TANF recidivism.
Dynamics in prestressed media with moving phase boundaries: a continuum theory of failure in solids

Spontaneous failure in a solid medium is described as a localized transition of the material from one physical state to another, characterized in part by contrasting rheological properties and density. Such a process is viewed as a local disordering of the relatively ordered structure of the solid due to any variety of causes, such as massive microfracturing or shear melting, and can be confined to a very thin zone, but nevertheless of finite volume such that a volumetric transition energy can be defined. This leads to the description of failure as a generalized phase transition in a prestressed continuum, with instability and transition zone growth being driven by the energy contributions from the relaxation of stress in the surrounding medium. Direct application of mass, momentum and energy conservation to such a generalized phase transition leads to ‘jump’ conditions specified on the growing boundary surface of the transition zone, that relate the rupture growth to discontinuous changes in the dynamic field variables across the failure zone boundary. These field discontinuities are, in turn, related to the localized changes in physical properties induced by failure. Dynamical conditions for rapid spontaneous failure growth in a stressed medium are investigated in some detail, and we find that the failure boundary growth can be simply expressed in terms of energy ‘failure condition’ and a dynamic growth condition specifying the rupture velocity. These results imply that the integral energy change associated with earthquakes is in the range 10^4−10^6 erg/g. Further the failure growth rate is shown to be expressible in terms of the rheological properties of the material before and after failure. For shear melting resulting in a low viscosity fluid, for example, the rupture velocity will be near the shear velocity of the original material. A general Green's function solution for the radiation due to stress relaxation in the medium surrounding the growing failure zone is given and provides the basis for detailed computations of the strain or displacement field changes due to spontaneous failure processes. In particular, it is shown that the jump conditions for the growing transition zone boundary appear naturally as surface integral terms over the boundary. Since these boundary conditions contain the failure rate explicitly, then these terms include effects that have not been represented in previous integral representations of the radiation field resulting from failure. Further, we show that the formal Green's integral representation for the dynamical wave field can be used with known, simple Green's functions to generate approximate solutions for complex failure processes occurring in media with inhomogeneous material properties and prestress.
ANALYSIS AND FUNCTION OF TRANSCRIPTIONAL REGULATORY ELEMENTS: Insights from Drosophila

Analysis of gene expression is assuming an increasingly important role in elucidating the molecular basis of insect biology. Transcriptional regulation of gene expression is directed by a variety of cis-acting DNA elements that control spatial and temporal patterns of expression. This review summarizes current knowledge about properties of transcriptional regulatory elements, based largely on research in Drosophila melanogaster, and outlines ways that new technologies are providing tools to facilitate the study of transcriptional regulatory elements in other insects.
Efficient computation of the time-domain TE plane-wave reflection coefficient

An efficient method for the calculation of the transient reflection coefficient for TM plane wave reflection from a conducting half space is given. The corrected series form, which is valid for angles of incidence less than the Brewster angle, is shown to converge within a few terms for typical values of material parameters.
Repurposing Itraconazole as a Treatment for Advanced Prostate Cancer: A Noncomparative Randomized Phase II Trial in Men With Metastatic Castration-Resistant Prostate Cancer

BACKGROUND The antifungal drug itraconazole inhibits angiogenesis and Hedgehog signaling and delays tumor growth in murine prostate cancer xenograft models. We conducted a noncomparative, randomized, phase II study evaluating the antitumor efficacy of two doses of oral itraconazole in men with metastatic prostate cancer. PATIENTS AND METHODS We randomly assigned 46 men with chemotherapy-naive metastatic castration-resistant prostate cancer (CRPC) to receive low-dose (200 mg/day) or high-dose (600 mg/day) itraconazole until disease progression or unacceptable toxicity. The primary endpoint was the prostate-specific antigen (PSA) progression-free survival (PPFS) rate at 24 weeks; a 45% success rate in either arm was prespecified as constituting clinical significance. Secondary endpoints included the progression-free survival (PFS) rate and PSA response rate (Prostate Cancer Working Group criteria). Exploratory outcomes included circulating tumor cell (CTC) enumeration, serum androgen measurements, as well as pharmacokinetic and pharmacodynamic analyses. RESULTS The high-dose arm enrolled to completion (n = 29), but the low-dose arm closed early (n = 17) because of a prespecified futility rule. The PPFS rates at 24 weeks were 11.8% in the low-dose arm and 48.0% in the high-dose arm. The median PFS times were 11.9 weeks and 35.9 weeks, respectively. PSA response rates were 0% and 14.3%, respectively. In addition, itraconazole had favorable effects on CTC counts, and it suppressed Hedgehog signaling in skin biopsy samples. Itraconazole did not reduce serum testosterone or dehydroepiandrostenedione sulfate levels. Common toxicities included fatigue, nausea, anorexia, rash, and a syndrome of hypokalemia, hypertension, and edema. CONCLUSION High-dose itraconazole (600 mg/day) has modest antitumor activity in men with metastatic CRPC that is not mediated by testosterone suppression.
Surgical Management of Dry Eyes

There are numerous approaches for surgical management of dry eyes, and the appropriate intervention should be guided by the primary etiology of the dry eye symptoms. Oftentimes, the various etiologies can result in overlapping problems, which present as similar symptoms. These etiologies should be grouped into categories that address the primary problem: tear volume underproduction, tear film instability, mechanical disturbance, and ocular surface damage.