cssi/SciSciGPT-SciSciNet huggingface dataset:
Gastric lymphoid follicles in Helicobacter pylori infection: frequency, distribution, and response to triple therapy.

To determine the prevalence and distribution of gastric lymphoid follicles in patients with Helicobacter pylori infection, to evaluate their relationship with gastroduodenal pathology, and to assess their evolution after eradication of H pylori, mapped gastric biopsy specimens were obtained from 20 H pylori-negative normal volunteers, 25 asymptomatic volunteers with H pylori infection and no ulcer disease, 21 duodenal ulcer patients, and 16 patients with gastric ulcer. Nine infected subjects were treated by triple therapy, and biopsy specimens were obtained at 1, 4, and 12 months posttreatment. Lymphoid follicles were counted and other histologic features were scored semiquantitatively. None of the noninfected subjects had lymphoid follicles. All subjects with H pylori had follicles, which were more numerous in the antrum than in the corpus (P < .001) and on the lesser rather than on the greater curvature (P = .003). Ulcer patients had greater numbers of follicles than asymptomatic infected volunteers (P < .05). There was no relationship between number or distribution of follicles and patients' age, sex, or other mucosal inflammatory responses (except for numbers of lymphocytes: r = .785, P < .001), intensity of H pylori infection, or intestinal metaplasia. Eradication of H pylori resulted in a slow decrease (but not in the disappearance) of lymphoid follicles in all patients. Our results indicate that the careful examination of multiple specimens will reveal lymphoid follicles in the gastric mucosa of all patients with H pylori infection. The normal stomach does not contain mucosa-associated lymphoid tissue, and this study supports the concept that H pylori may be a precursor in the development of primary gastric lymphomas.
Sonographic characteristics of linear nevus sebaceous sequence

Linear nevus sebaceous is a hamartomatous lesion most commonly seen on the face and head of infants and diagnosed after birth. We describe a patient with sonographic findings of linear nevus sebaceous seen initially at 30 weeks' gestation. Other associated sonographic findings include macrocephaly and polyhydramnios. When associated with a midline soft tissue mass, these findings are highly suggestive of the presence of congenital linear nevus sebaceous sequence.
Ultrathin silicon oxide films deposited by synchrotron irradiation of condensed layers of silanes and water

Silicon oxide and carbide ultrathin films (less than 50 A thick) were grown at rates of up to 1 A s−1 using a previously developed technique. To form silicon oxide, a mixture of silane or tetramethylsilane and water was condensed onto a metal surface, which was then exposed to either broad-band or monochromatized synchrotron radiation. Characterization by soft X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure showed that clean, near-stoichiometric films of self-limited thickness were grown. The results also suggested that the reactions leading to film growth were predominantly excited by electrons produced by photon absorption in the substrate.
“Race records” and “hillbilly music”: institutional origins of racial categories in the American commercial recording industry

Abstract The relationship between artistic boundaries and social boundaries is examined through the construction of the musical categories of “race records” and “hillbilly music” in commercial music. Evidence shows that these categories were less a part of a larger racial project than the institutional and business practices of the early recording industry, though once established they became self-perpetuating.
Electroless Deposition of NiB on 15 Inch Glass Substrates for the Fabrication of Transistor Gates for Liquid Crystal Displays

The thin-film transistor (TFT) array of liquid-crystal displays (LCDs) comprises a number of metallic, semiconducting, and insulating layers, which need to be deposited and patterned accurately with very high yields on a (large) glass substrate. We are exploring how to fabricate the gate metal lines of the TFT array in an entirely new and potentially cost-effective wayby depositing the metal layer of the TFT array using electroless deposition (ELD) and by patterning the gates using microcontact printing (μCP). To achieve this goal, we separately explore first the plating conditions to deposit a gate metal on 15 in. glass substrates, and second the printing process to finally combine them later in the work. Here, we review in depth the metallization of the glass by ELD of NiB as gate material, and we demonstrate the patterning of the gate layer using a conventional photoengraving process (PEP, i.e., photolithography and wet etching). We selected NiB because this material can fulfill the conductivity requir...
Diffusion tensor imaging at low SNR: nonmonotonic behaviors of tensor contrasts

Diffusion tensor imaging (DTI) provides measurements of directional diffusivities and has been widely used to characterize changes in the tissue microarchitecture of the brain. DTI is gaining prominence in applications outside of the brain, where resolution, motion and short T2 values often limit the achievable signal-to-noise ratio (SNR). Consequently, it is important to revisit the topic of tensor estimation in low-SNR regimes. A theoretical framework is developed to model noise in DTI, and by using simulations based on this theory, the degree to which the noise, tensor estimation method and acquisition protocol affect tensor-derived quantities, such as fractional anisotropy and apparent diffusion coefficient, is clarified. These results are then validated against clinical data. It is shown that reliability of tensor contrasts depends on the noise level, estimation method, diffusion-weighting scheme and underlying anatomy. The propensity for bias and errors does not monotonically increase with noise. Comparative results are shown in both graphical and tabular forms, so that decisions about suitable acquisition protocols and processing methods can be made on a case-by-case basis without exhaustive experimentation.
The effects of macroinvertebrate taxonomic resolution in large landscape bioassessments: an example from the Mid-Atlantic Highlands, U.S.A.

Summary 1. During late spring 1993–1995, the U.S. Environmental Protection Agency's Environmental Monitoring and Assessment Program (EMAP) sampled 490 wadeable streams in the mid-Atlantic Highlands (MAH) of the U.S. for a variety of physical, chemical and biological indicators of environmental condition. We used the resulting data set to evaluate the importance of differing levels of macroinvertebrate taxonomic resolution in bioassessments by comparing the ability of family versus genus to detect differences among sites classified by type and magnitude of human impact and by stream size. We divided the MAH into two physiographic regions: the Appalachian Plateau where mine drainage (MD) and acidic deposition are major stressors, and the Ridge and Valley where nutrient enrichment is a major stressor. Stream sites were classified into three or four impact classes based on water chemistry and habitat. We used stream order (first to third Strahler order) in each region as a measure of stream size. Ordination, 2 × 2 chi-square and biotic metrics were used to compare the ability of family and genus to detect differences among both stressor and size classes. 2. With one notable exception, there were only a small number of different genera per family (interquartile range = 1–4). Family Chironomidae, however, contained 123 different genera. As a result, significant information loss occurred when this group was only classified to family. The family Chironomidae did not discriminate among the predefined classes but many chironomid genera did: by chi-square analysis, 10 and 28 chironomid genera were significant in discriminating MD and nutrient impacts, respectively. 3. Family and genus data were similar in their ability to distinguish among the coarse impacts (e.g. most severe versus least severe impact classes) for all cases. Though genus data in many cases distinguished the subtler differences (e.g. mixed/moderate impacts versus high or low impacts) better than family, differences in significance levels between family and genus analyses were relatively minor. However, genus data detected differences among stream orders in ordination analyses that were not revealed at the family level. In the ordinations, both family and genus levels of analysis responded to similar suites of environmental variables. 4. Our results suggest that identification to the family level is sufficient for many bioassessment purposes. However, identifications to genus do provide more information in genera-rich families like Chironomidae. Genus or finer levels of identification are important for investigating natural history, stream ecology, biodiversity and indicator species. Decisions about the taxonomic level of identification need to be study specific and depend on available resources (cost) and study objectives.
TOLERANCE TO NITROGENOUS EXPLOSIVES AND METABOLISM OF TNT BY CELL SUSPENSIONS OF DATURA INNOXIA

Cell suspension cultures of Datura innoxia were incubated in the presence of the nitro-substituted explosives 2,4,6-trinitrotoluene (TNT), 1,3,5-trinitro-1,3,5-triazine (RDX), and 1,3,5,7-tetranitro-1,3,5,7-tetraazocyclooctane (HMX). Cellular tolerance levels and TNT biotransformation kinetics were examined. Tolerance to TNT varied as cell suspensions aged. Concentrations of RDX or HMX in excess of reported solubility limits produced no observable changes in cell viability. GC/MS analysis of TNT-treated cell media and cell lysates revealed rapid removal of TNT. Within 12 h, less than 1% of the initial TNT remained in the growth medium. Aminodinitrotoluenes (ADNTs), known metabolites of TNT, accumulated transiently in cell lysates, and to a lesser extent in cell media. ADNT concentrations started to decrease after 3 h. After 12 h, less than 5% of the initial TNT could be detected as ADNT. Total ADNTs never exceeded 26% of initial TNT, suggesting that additional biotransformation steps also occurred. No other nitroaromatics were detected. A pseudo-first order rate constant for TNT clearance was calculated, k=0.40 h−1. D. innoxia cell suspension cultures demonstrated virtually complete clearance of TNT and of subsequent ADNT metabolites in less than 12 h. This rapid metabolism of nitroaromatics by the Datura cell suspension system indicates the utility of this system for further molecular and biochemical studies.
Evaluation of genetic mutations associated with Mycobacterium tuberculosis resistance to amikacin, kanamycin and capreomycin: a systematic review.

Background Rapid molecular diagnostics for detecting multidrug-resistant and extensively drug-resistant tuberculosis (M/XDR-TB) primarily identify mutations in Mycobacterium tuberculosis (Mtb) genes associated with drug resistance. Their accuracy, however, is dependent largely on the strength of the association between a specific mutation and the phenotypic resistance of the isolate with that mutation, which is not always 100%. While this relationship is well established and reliable for first-line anti-TB drugs, rifampin and isoniazid, it is less well-studied and understood for second-line, injectable drugs, amikacin (AMK), kanamycin (KAN) and capreomycin (CAP). Methodology/Principal Findings We conducted a systematic review of all published studies evaluating Mtb mutations associated with resistance to AMK, KAN, CAP in order to characterize the diversity and frequency of mutations as well as describe the strength of the association between specific mutations and phenotypic resistance in global populations. Our objective was to determine the potential utility and reliability of these mutations as diagnostic markers for detecting AMK, KAN and CAP resistance. Mutation data was reviewed for 1,585 unique clinical isolates from four continents and over 18 countries. Mutations in the rrs, tlyA, eis promoter and gidB genes were associated with AMK, KAN and/or CAP resistance. Conclusions/Significance The rrs A1401G mutation was present in the majority of AMK, KAN and CAP resistant Mtb strains reviewed, but was also found in 7% of CAP susceptible strains. The 1401 mutation alone, however, was not found with sufficient frequency to detect more than 70–80% of global Mtb strains resistant to AMK and CAP, and 60% of strains resistant to KAN. Additional mutations in the rrs, eis promoter, tlyA and gidB genes appear to be associated with resistance and could improve sensitivity and specificity of future diagnostics.
The effects of hedges and hesitations on impression formation in a simulated courtroom context

The purpose of this research was to refine the concepts of powerful and powerless speech styles by investigating evaluative reactions to hedges and hesitations in a simulated trial context. We asked whether hedges, hesitations, and respondent sex would interact to affect listeners’ evaluations of a speaker. We also asked whether these language variables affected perceptions of guilt. We found that a low level of hedges and hesitations produced the most positive evaluations of authoritativeness and attractiveness. The study also revealed that these variables affected perceptions of guilt.
Nonlinear Antiplane Response of Rigid Inclusion to Incident Stress Wave

The response of a rigid cylinder embedded in a homogeneous, isotropic half space to a step function incident stress wave is investigated. The boundary integral equation method is used directly in the time domain where two types of interfacial stresses are considered: In the first case, the interfacial stresses are not permitted to exceed a prescribed constant maximum value (elastic‐perfectly plastic interface behaviour); in the second case, a friction interface is considered where the limiting stresses vary along the interface according to the normal interfacial pressure. The angular distribution of shear stress at different times are examined to illustrate the redistribution of interfacial stresses. The relative displacements between the cylinder and half space are also presented.
Choice of prosthetic heart valve for adult patients

Abstract This review summarizes the major long-term (≥10 to 15 years) patient outcomes after insertion of many Food and Drug Administration approved prosthetic heart valves (PHV). Mechanical PHV was associated with a better survival (p AVR) and, therefore, for replacement of the PHV. Thromboembolism and bleeding rate were higher with mechanical PHV. Mortality after AVR and MVR is high at 10 to 15 years because of the associated comorbid conditions and older age of patients. Outcomes with “new” good valves are similar to that with “older” good valves. Complication rates of thromboembolism, bleeding, endocarditis, and leak vary widely; the rates of these complications are not different among different mechanical PHV and among different bioprosthetic PHV. Structural valve deterioration is rare with mechanical PHV. Structural valve deterioration of bioprosthesis after MVR is higher than after AVR; after AVR, homografts and bioprosthesis have similar rates of SVD. The exact rate of SVD of the pulmonary autograft is uncertain. Valve prosthesis-patient mismatch is clinically important when it is severe and in selected patients when it is moderate. Bioprosthesis have a low rate of SVD in the older patient and, thus, are the PHV of choice for AVR in patients ≥60 to 65 years of age and for MVR in patients ≥65 to 70 years of age; in younger patients mechanical valves are the PHV of choice. In individual patients there may be exceptions to these general rules.
On Being Insecure about the Assessment of Attachment Styles

The psychometric properties of subscores from Collins and Read's (1990) Adult Attachment Scale (AAS, Ns = 61 gay, 42 lesbian, and 155 heterosexual couples) and Griffin and Bartholomew's (1994b) Relationship Scales Questionnaire (RSQ, N = 33 gay, 52 lesbian, and 79 heterosexual couples) were examined. Type of couple did not moderate any findings. Dependency and Closeness emerged as reliable factors from the AAS, but, with controls for the Big Five personality factors, neither attachment style accounted for unique variability in relationship satisfaction, and only Closeness accounted for unique variability in relationship commitment. Closeness exerted an indirect effect on commitment through both expressiveness and dysfunctional beliefs regarding relationship standards. Avoidance and Anxiety emerged as reliable factors from the RSQ, but, with controls for the six facets of Neuroticism, only Anxiety accounted for variability in satisfaction and commitment. Anxiety exerted an indirect effect on satisfaction a...
Identification of Potential Therapeutic Drugs for Huntington's Disease using Caenorhabditis elegans

Background The prolonged time course of Huntington's disease (HD) neurodegeneration increases both the time and cost of testing potential therapeutic compounds in mammalian models. An alternative is to initially assess the efficacy of compounds in invertebrate models, reducing time of testing from months to days. Methodology/Principal Findings We screened candidate therapeutic compounds that were identified previously in cell culture/animal studies in a C. elegans HD model and found that two FDA approved drugs, lithium chloride and mithramycin, independently and in combination suppressed HD neurotoxicity. Aging is a critical contributor to late onset neurodegenerative diseases. Using a genetic strategy and a novel assay, we demonstrate that lithium chloride and mithramycin remain neuroprotective independent of activity of the forkhead transcription factor DAF-16, which mediates the effects of the insulin-like signaling pathway on aging. Conclusions/Significance These results suggest that pathways involved in polyglutamine-induced degeneration are distinct from specific aging pathways. The assays presented here will be useful for rapid and inexpensive testing of other potential HD drugs and elucidating pathways of drug action. Additionally, the neuroprotection conferred by lithium chloride and mithramycin suggests that these drugs may be useful for polyglutamine disease therapy.
Towards the development of an intimate partner violence screening tool for gay and bisexual men.

Introduction: Recent research suggests that gay and bisexual men experience intimate partner violence (IPV) at rates comparable to heterosexual women. However, current screening tools used to identify persons experiencing IPV were largely created for use with heterosexual women. Given the high prevalence of IPV among gay and bisexual men in the United States, the lack of IPV screening tools that reflect the lived realities of gay and bisexual men is problematic. This paper describes the development of a short-form IPV screening tool intended to be used with gay and bisexual men. Methods: A novel definition of IPV, informed by formative Focus Group Discussions, was derived from a quantitative survey of approximately 1,100 venue-recruited gay and bisexual men. From this new definition, a draft IPV screening tool was created. After expert review (n=13) and cognitive interviews with gay and bisexual men (n=47), a screening tool of six questions was finalized. A national, online-recruited sample (n=822) was used to compare rates of IPV identified by the novel tool and current standard tools. Results: The six-item, short-form tool created through the six-stage research process captured a significantly higher prevalence of recent experience of IPV compared to a current and commonly used screening tool (30.7% versus 7.5%, p<0.05). The novel short-form tool described additional domains of IPV not currently found in screening tools, including monitoring behaviors, controlling behaviors, and HIV-related IPV. The screener takes less than five minutes to complete and is 6th grade reading level. Conclusion: Gay and bisexual men experiencing IPV must first be identified before services can reach them. Given emergent literature that demonstrates the high prevalence of IPV among gay and bisexual men and the known adverse health sequela of experiencing IPV, this novel screening tool may allow for the quick identification of men experiencing IPV and the opportunity for referrals for the synergistic management of IPV. Future work should focus on implementing this tool in primary or acute care settings in order to determine its acceptability and its feasibility of use more broadly. [West J Emerg Med. 2013;14(4):391–401.]
A spatial analysis of mental retardation of unknown cause and maternal residence during pregnancy

Spatial analysis is useful for the identification of areas of elevated risk of adverse health outcomes and generation of hypotheses. Identification of clusters based on maternal residence during pregnancy provides an important tool to investigate risk exposures. However, even though mental retardation (MR) is a substantial public health problem, there are no previous analyses of spatial clustering of childhood MR using individual case data. In this paper, we examine the use of the Bayesian hierarchical modeling approach in the analysis of MR clustering. We used data from South Carolina Medicaid and birth certificates, in which address codes for each month of pregnancy are available. MR cases with unknown cause were identified in the study population. A Bayesian local likelihood cluster modeling technique was applied to compute the relative risk of MR and its corresponding P-value for each geo-coded location, and the P-value surface was contoured as a heat image to identify the MR clusters. The characteristics of the study population were analyzed using chi-square tests and the results confirm that clustering does occur for MR. The shapes of the identified MR clusters were found to be irregular and the observed MR rate in the identified MR cluster area was found to be double the rate for the larger South Carolina region. The descriptive analysis of study population characteristics showed that the children with MR were more likely to be male and had mothers who were older than 34 years at the time of birth as well as being African American, preterm and of low birth weight compared to children without MR.
SPIDER FAUNA OF SUGAR MAPLE AND WHITE ASH IN NORTHERN AND CENTRAL NEW YORK STATE

Abstract The purpose of this study was to identify spiders associated with foliage of sugar maple, Acer saccharum Marsh. and white ash, Fraxinus americana L., and to investigate their distribution and relative abundance within the crowns of these two northern hardwoods. Spiders were collected during June through August 1995 from the lower and mid-crowns of ten dominant/codominant sugar maples, five white ash and understory sapling and herbaceous foliage ≤2 m from the ground in a northern hardwood stand in Cortland County New York. These samples were compared for differences in species composition and density (number/25 leaf-clusters; number/100 g dry leaf weight). The spider fauna obtained from this intensive sample was compared to that of an extensive, 20-year survey from the mid-crown of sugar maple in 15 northern hardwood stands in northern New York (St. Lawrence and Lewis Counties). The intensive overstory collection (1995) from maple provided 694 specimens (7 families, 11 genera, 13 species). The dom...
Abandoning Informed Consent: An Idea Whose Time Has Not Yet Come

In a recent critique of informed consent, Robert Veatch argues that the practice is in principle unable to attain the goals for which it was developed. We argue that Veatch's focus on the theoretical impossibility of determining patients' best interests is misapplied to the practical discipline of medicine, and that he wrongly assumes that the patient-physician communication fails to provide the knowledge needed to insure the patient's best interests. We further argue that Veatch's suggested alternative, value-based patient-professional pairing, is, on his own terms, impossible to implement. Finally, we reexamine the philosophical and practical justifications for informed consent and conclude that the practice should be retained.
Relation of tissue displacement and strain to invasively determined right ventricular stroke volume.

The role of echocardiography in the clinical assessment of right ventricular (RV) systolic function remains limited. Limited data exist on the potential use of newer techniques for RV function assessment. Conventional echocardiography and tissue Doppler echocardiography (TDE) were performed during right-sided cardiac catheterization in 46 patients. Thermodilution or the Fick-derived RV stroke volume indexed (RVSVI) indexed to body surface area was used as the reference standard. Univariate and multivariate regression analyses were used to test correlations between RVSVI and various echocardiographic and TDE-derived parameters. In a subset of 12 subjects, changes in echocardiographic and TDE variables to reduced afterload from intravenous epoprostenol were measured. TDE-derived RV tissue displacement and systolic strain best predicted the RVSVI (r = 0.63, p = 0.001; r = 0.48, p = 0.002, respectively). The prediction improved after adjustment for tricuspid regurgitation jet vena contracta width (r = 0.74, p or =30 ml/m(2) as normal, a RV displacement cutoff of 15 mm yielded a sensitivity of 100% and a specificity of 41% for RV dysfunction, and an RV systolic strain cutoff of 20% yielded a sensitivity of 91% and a specificity of 63%. The percentage change of RV systolic displacement correlated well with the percentage change of RVSVI after epoprostenol infusion (r = 0.75, p < 0.001). In conclusion, TDE-derived RV displacement and strain closely correlate with RVSVI and appropriately track load-related changes in RV function. These new parameters may help provide the noninvasive, quantitative assessment of RV function.
The Veil of Esteem: On Seeing Oneself Being Seen (Part Three: A Loan)

This essay is the third and final of a three-part series titled “The Veil of Esteem: On Seeing Oneself Being Seen.” Inspired by Walter Benjamin’s “reflection through vignette” method, I inquire into the notions and interconnections between memory and esteem. Esteem is the truth of oneself through the eyes of the other, and any truth of esteem must be told from the perspective of that other, through the spectating other. Thus, I find that any story of esteem is veiled. This final part, A Loan, posits that our unified recollection is a fiction culled from fragmented truths. This isn’t all bad. It’s the only way we can shape the heterogeneous multiplicity of happy accidents, shaping it in a poetic way. We need to look back from the future to cultivate things that may turn out to be desirable that are happening now. In other words, we can’t leave accident to chance. Otherwise, we’re left with a nothingness that’s but a lack of vision. The story is narrated not as a representation of a person or of people, but...
Adaptive significance of evergreen vs. deciduous leaves : solving the triple paradox

Patterns in the dominance of evergreen vs. deciduous plants have long interested ecologists, biogeographers, and global modellers. But previous models to account for these patterns have signifi cant weaknesses. Bottom-up, mechanistic models – based on physiology, competition, and natural selection – have often been non-quantitative or restricted to a small range of habitats, and almost all have ignored belowground costs and whole-plant integration. Top-down, ecosystem-based models have succeeded in quantitatively reproducing several patterns, but rely partly on empirically derived constants and thresholds that lack a mechanistic explanation. It is generally recognized that seasonal drought can favor deciduous leaves, and that infertile soils can favor long-lived evergreen leaves. But no model has yet explained three great paradoxes, involving dominance by 1) evergreens in highly seasonal, boreal forests, 2) deciduous larch in many nutrient-poor peatlands, and 3) evergreen leaf-exchangers in nutrient-poor subtropical forests, even though they shed their leaves just as frequently as deciduous species. This paper outlines a generalized optimality model to account for these and other patterns in leaf longevity and phenology, based on maximizing whole-plant carbon gain or height growth, and building on recent advances in our understanding of the quantitative relationships of leaf photosynthesis, nitrogen content, and mass per unit area to leaf life-span. Only a whole-plant approach can explain evergreen dominance under realistic ecological conditions, or account for the boreal paradox, the larch paradox, the leaf-exchanger paradox, and expected shifts in shade tolerance associated with leaf phenology. Poor soils favor evergreens not merely by increasing the costs of nutrient acquisition, but also by depressing the maximum rate of photosynthesis and thus the seasonal contrast in photosynthetic return between leaves adapted to favorable vs. unfavorable conditions. The dominance of evergreens in western North America beyond the coastal zone of mild winters and winter rainfall appears related to the unusually long photosynthetic season for evergreen vs. deciduous plants there. Future models for optimal leaf phenology must incorporate differences between evergreen and deciduous plants in allocation to photosynthetic vs. non-photosynthetic tissue, rooting depth, stem allometry, xylem anatomy, and exposure to herbivores and leaching, and analyze how these differences interact with the photosynthetic rate, transpiration, and nutrient demands of leaves with different life-spans to affect rates of height growth in specifi c microsites.
An Evaluation of Low-Energy X-Ray and Cobalt-60 Irradiations of MOS Transistors

An evaluation of methodologies for irradiating MOS transistors with low-energy x-ray and Co-60 sources has been performed. We find that comparisons of voltage shifts produced by bulk trapped charge and interface states in MOS transistors irradiated using two different low energy x-ray sources (an ARACOR 10 keV W source and an 8 keV Cu source) agree to within better than 30 percent. This quality of agreement is similar in magnitude to that between MOS devices irradiated by different Co-60 sources. In contrast, the measurements indicate that interlaboratory comparisons of ratios of shifts produced by x-ray and Co-60 sources can lead to differences in ratios as large as a factor of ~1.7. Improved electron-hole recombination data for oxides is presented. This recombination correction, in conjunction with a correction for interface dose enhancement, is used to predict the ratios of shifts produced by x-ray and Co-60 sources. However, the results show that corrections for electron-hole recombination and interface dose enhancement do not, by themselves, adequately predict the field dependent behavior of these transistors.
L-Selectin ligands that are O-glycoprotease resistant and distinct from MECA-79 antigen are sufficient for tethering and rolling of lymphocytes on human high endothelial venules.

During the process of lymphocyte recirculation, lymphocytes bind via L-selectin to sulfated sialyl-Lewisx (sLex)–containing carbohydrate ligands expressed on the surface of high endothelial venules (HEV). We have examined the expression of sLex on HEV using a panel of mAbs specific for sLex and sLex-related structures, and have examined the function of different sLex-bearing structures using an in vitro assay of lymphocyte rolling on HEV. We report that three sLex mAbs, 2F3, 2H5, and CSLEX-1, previously noted to bind with high affinity to glycolipid-linked sLex, vary in their ability to stain HEV in different lymphoid tissues and bind differentially to O-linked versus N-linked sLex on glycoproteins. Treatment of tissue sections with neuraminidase abolished staining with all three mAbs but slightly increased staining with MECA-79, a mAb to a sulfation-dependent HEV-associated carbohydrate determinant. Treatment of tissue sections with O -sialoglycoprotease under conditions that removed the vast majority of MECA-79 staining, only partially reduced staining with the 2F3 and 2H5 mAbs. Using a novel rolling assay in which cells bind under flow to HEV of frozen tissue sections, we demonstrate that a pool of O -sialoglycoprotease–resistant molecules is present on HEV that is sufficient for attachment and rolling of lymphocytes via L-selectin. This interaction is not inhibited by the mAb MECA-79. Furthermore, MECA-79 mAb blocks binding to untreated sections by only 30%, whereas the sLex mAb 2H5 blocks binding by ∼60% and a combination of MECA-79 and 2H5 mAb blocks binding by 75%. We conclude that a pool of O -glycoprotease-resistant sLex-like L-selectin ligands exist on human HEV that is distinct from the mucin-associated moieties recognized by MECA-79 mAb. We postulate that these ligands may participate in lymphocyte binding to HEV.
Identification of 2-hydroxy fatty acids in complex mixtures of fatty acid methyl esters by Mass chromatography.

Repetitive mass spectrometric scanning of gas-liquid chromatographic effuents at short (6 to 15 s) intervals, with a digital computer for data acquisition, reduction and display, allows plots (mass chromatograms) to be made of the intensities of specific ion fragments. Plotting of m/e values characteristic for certain classes of compounds and/or individual components reveals the location of constituents in the gas chromatogram. This technique was applied to the identification of trimethylsilyl derivatives of unsubstituted and 2-hydroxy fatty acid methyl esters in mixtures obtained from acid catalyzed methanolysis of cat brain galactocerebrosides. The plot of total ionization intensity vs scan number generated the gas-liquid chromatogram. Mass chromatograms of m/e 73 and [M – 59]+ gave the locations in the gas-liquid chromatogram of the hydroxy fatty acid derivatives, and mass chromatograms of m/e 74 and [M]+ located the unsubstituted fatty acid derivatives. Integrated ion intensities were used to determine the composition of the mixture of fatty acids.
Starch Measurement in Plant Tissue Using Enzymatic Hydrolysis

This work explored completeness of starch hydrolysis in situ in relation to degree of gelatinization, starch content of tissue, evailable enzyme activity, and time allowed for hydrolysis. Maximum hydrolysis of starch in lyophilized red oak (Quercus rubra L.) root tissue with purified Diazyme (amyloglucosidase) or Clarase (Takadiastase) required high enzyme activity (2.4 U Diazyme or 48 U Clarase per mg starch). Results suggested that at least 70 U Clarase or 5 U Diazyme should be used per mg starch in routine analyses. Neither prolonging gelatinization (more than 15 min) nor hydrolysis (more than 24 to 48 lh) offset inadequate starch hydrolysis caused by insufficient enzyme activity. Starch was completely hydrolyzed in situ after 48 h without gelatinization by 5 U of Diazyme per mg starch. Tissue weight (5 to 100 mg) had no effect on starch hydrolysis by sufficient enzyme. Methanol: chloroform: water (12:5:3 by volume) freed tissues of solubles before starch hydrolysis. No interference with the glucose oxidase analysis of hydrolysates was encountered. In addition, the pigment free methanol–water fractions (soluble sugars, amino acids, organic acids) and chloroform fractions (lipids and pigments) were available or further analysis. Results obtained with red oak were verified with issue from other species such as jack pine (Pinus banksiana lamb.) and white spruce (Picea glauca (Moench) Voss). The resulting technique simply and reliably measured less than 5% starch in 5 mg lyophilized tissue, with a minimum of sample manipulation.
P01.24. Central processing by electroacupuncture of vasodepression and bradycardia reflex responses

Purpose Electroacupuncture (EA) at P5-6 reduces sympathoexcitatory blood pressure (BP) reflex responses. Gammaaminobutyric acid (GABA) receptors in the brainstem region rostral ventral lateral medulla (rVLM) contribute to modulation of sympathoexcitatory visceral reflexes during EA. Gastric distension in hypercapnic acidotic rats, by activating both sympathetic and vagal afferents, decreases BP and heart rate (HR) through a GABAA mechanism in the rVLM. This study investigated the hypothesis that EA modulates gastric induced hemodynamic depressor responses through actions in nuclei that process both sympathetic and parasympathetic outflow. Methods Anesthetized and hypercapnic acidotic induced rats were used to examine the central processing of the actions of EA. An unstressed 2-cm diameter latex balloon attached to a polyurethane tube was inserted into the stomach through the mouth and esophagus to induce gastric distention. Acupuncture needles were placed near the wrist at P5-6 acupoints for 30-min EA (2 Hz, 0.2-0.4 mA, 0.5 ms). Results We observed repeatable decreases in BP and HR with gastric distention every 10 min. Bilateral EA at P5-6 for 30 min reversed the hypotensive response from -26±3 to -6±1 mmHg and the bradycardia from -35±11 to -10 ±3 beats/min for over 70 min. EA’s action on decreased BP and HR, respectively, was inhibited by microinjection of gabazine, a GABAA receptor antagonist, into the caudal–VLM (cVLM) or the nucleus ambiguus (NAmb). Gabazine microinjected into the rVLM reversed EA action on both depressor and bradycardia responses. Conclusion Thus, EA through GABAA receptor mechanisms modulates reflex sympathoinhibition and vagal excitation leading to cardiovascular depression through actions in the rVLM, cVLM and NAmb. These data indicate that EA can normalize elevated and depressed blood pressure.
Development of parallel dip pen nanolithography probe arrays for high throughput nanolithography

Dip Pen Nanolithography (DPN) is a lithographic technique that allows direct deposition of chemicals, metals, biological macromolecules, and other molecular “inks” with nanometer dimensions and precision. This paper addresses recent developments in the design and demonstration of high-density multiprobe DPN arrays. High-density arrays increase the process throughput over individual atomic force microscope (AFM) probes and are easier to use than arrays of undiced commercial probes. We have demonstrated passive arrays made of silicon (8 probes, 310 μm tip-to-tip spacing) and silicon nitride (32 probes, 100 μm tip-to-tip spacing). We have also demonstrated silicon nitride “active” arrays (10 probes, 100 μm tip-to-tip spacing) that have embedded thermal actuators for individual probe control. An optimization model for these devices, based on a generalized multilayer thermal actuator, is also described.
Diophantine Frequency Synthesis A Number Theory Approach to Fine Frequency Synthesis

Diophantine Frequency Synthesis1 is a new approach to fine-step2, fast-hopping, low-spurs frequency synthesis that is based on mathematical properties of integer numbers and linear Diophantine equations. Diophantine Frequency Synthesis overcomes the constraining relation between frequency step and phase-comparator frequency inherent in conventional phase-locked loops. It leads to fine-step, fast-hopping, modular-structured frequency synthesizers with potentially very low spurs, especially in the vicinity of the carrier.
Copepod grazing impact on the trophic structure of the microbial assemblage of the San Pedro Channel, California

In August 2002 and March 2003 the trophic structure of the microbial assemblage from the San Pedro Channel, California was studied following the experimental alteration of the number of copepods. Changes in the abundance/biomass of microorganisms 80 μm, (ii) the presence of natural abundances of metazoa and (iii) the presence of an elevated number of copepods. Prokaryotes and small-sized eukaryotes (<4 μm) dominated plankton biomass during both experimental months. Diatoms numerically dominated the 10-80 μm plankton in August 2002, but ciliate and heterotrophic dinoflagellate biomass generally exceeded diatom biomass on both dates. Ingestion of protozooplankton (predominantly ciliates) contributed substantially to copepod daily carbon rations. The adult copepod assemblage removed 4.6 and 36% per day of the microzooplankton standing stocks (10-80 μm size fraction) in August and March, respectively. Elevated copepod grazing pressure on protozooplankton resulted in increased biomass of nanoplankton (<5 μm) presumably via a trophic cascade. Accordingly, the copepod-protozoan trophic link appears to be a key factor structuring the planktonic microbial assemblage in the San Pedro Channel.
Faith organizations and neighbourhood councils in Los Angeles

Following reforms to the city charter in 1999, the City of Los Angeles is currently implementing a city-wide system of neighbourhood councils that is intended to increase citizen participation in local governance and make city agencies more responsive to local needs. As neighbourhood council formation has gotten underway, faith organizations (FOs) have been identified as a promising resource for mobilizing involvement among groups that are traditionally underrepresented in the governance process. This article discusses the potential value of FOs' involvement in neighbourhood councils, as well as the likely obstacles to their participation, and reports preliminary findings from ongoing field research on neighbourhood council formation in Los Angeles. Given the diversity of religious missions and attitudes toward political involvement among American faith institutions we find it difficult to generalize about the likely role of the faith community in neighbourhood councils. However, the historical focus of Christian churches on issues of social justice as opposed to local community action, as well as the relative lack of participation by FOs in neighbourhood council formation to date, suggests that widespread involvement of FOs in the neighbourhood council process may be difficult to achieve. A targeted strategy of outreach and organizing directed toward those FOs most likely to participate seems required. Copyright © 2002 John Wiley & Sons, Ltd.
Interaction of Arabidopsis Trihelix-Domain Transcription Factors VFP3 and VFP5 with Agrobacterium Virulence Protein VirF

Agrobacterium is a natural genetic engineer of plants that exports several virulence proteins into host cells in order to take advantage of the cell machinery to facilitate transformation and support bacterial growth. One of these effectors is the F-box protein VirF, which presumably uses the host ubiquitin/proteasome system (UPS) to uncoat the packaging proteins from the invading bacterial T-DNA. By analogy to several other bacterial effectors, VirF most likely has several functions in the host cell and, therefore, several interacting partners among host proteins. Here we identify one such interactor, an Arabidopsis trihelix-domain transcription factor VFP3, and further show that its very close homolog VFP5 also interacted with VirF. Interestingly, interactions of VirF with either VFP3 or VFP5 did not activate the host UPS, suggesting that VirF might play other UPS-independent roles in bacterial infection. To better understand the potential scope of VFP3 function, we used RNAi to reduce expression of the VFP3 gene. Transcriptome profiling of these VFP3-silenced plants using high-throughput cDNA sequencing (RNA-seq) revealed that VFP3 substantially affected plant gene expression; specifically, 1,118 genes representing approximately 5% of all expressed genes were significantly either up- or down-regulated in the VFP3 RNAi line compared to wild-type Col-0 plants. Among the 507 up-regulated genes were genes implicated in the regulation of transcription, protein degradation, calcium signaling, and hormone metabolism, whereas the 611 down-regulated genes included those involved in redox regulation, light reactions of photosynthesis, and metabolism of lipids, amino acids, and cell wall. Overall, this pattern of changes in gene expression is characteristic of plants under stress. Thus, VFP3 likely plays an important role in controlling plant homeostasis.
Programmable Hybrid Integrated Circuit/Microfluidic Chips

The miniaturization of laboratory functions onto microfluidic chips is leading a paradigm shift in biotechnology, analogous to the transformation of electronics by the integrated circuit (IC) 50 years ago. The microfabricated pipes, pumps, valves, and mixers of microfluidics enable small volumes of reagents, samples, and individual living cells to be controlled on low-cost, portable chips. However, a microfluidic chip that can be programmed to perform the wide range of chemical and biological tasks required for medical and scientific analysis, akin to a microprocessor in electronics, remains a challenge. Here, we review work done by our group to develop hybrid IC/microfluidic chips that can simultaneously control thousands of living cells and picoliter volumes of fluid, enabling a wide variety of chemical and biological tasks.
The Dose-Response Relationship of bacillus Calmette-Guerin and Urothelial Carcinoma Cell Biology

Purpose: Attenuated mycobacterium bacillus Calmette-Guerin is widely used as intravesical immunotherapy of nonmuscle invasive urothelial carcinoma. Currently there are limited data on the relationship between bacillus Calmette-Guerin dose intensity and tumor response. We evaluated the dose-response relationship of bacillus Calmette-Guerin to nonmuscle invasive bladder cancer in vitro using urothelial carcinoma cell lines and in vivo using an orthotopic mouse model.Materials and Methods: Two human urothelial carcinoma cell lines were used to study the effect of bacillus Calmette-Guerin dose on the tumor cell response. Internalization, activation of signaling pathways, gene transactivation, cell viability, lactate dehydrogenase and HMGB1 release were study end points. An orthotopic tumor model was used to compare the effect of different doses on the antitumor efficacy of bacillus Calmette-Guerin.Results: Bacillus Calmette-Guerin internalization by urothelial carcinoma cells increased as a function of time a...
The effects of direct-to-consumer advertising of pharmaceuticals on adherence

We evaluate the effect of direct-to-consumer (DTC) advertising on adherence to the standard drug regimen for treating high cholesterol (statins). We use a rich panel data set from an insurance firm that includes all prescription drug purchases and insurance claims from 1997 to 2002.The data include the relevant demographic variables, including income. We match this data with unique data from Nielsen Monitor Plus on monthly DTC advertising expenditures by therapeutic category for all media types.The research strategy identifies individuals who have been diagnosed with specific chronic conditions and tracks whether adherence, as measured by purchases of specific prescription drugs within designated therapeutic classes, can be explained in part by the level and type of DTC advertising to which the individual was potentially exposed. Income has a positive effect on adherence. We find that advertising has a small negative effect on the average level of adherence. We find some suggestive evidence that this is b...
Expenditure and confidence: using daily data to identify shocks to consumer confidence

The importance of consumer confidence in stimulating economic activity is a disputed issue in macroeconomics. Do changes in confidence represent autonomous fluctuations in optimism, independent of information on economic fundamentals, or are they a reflection of economic news? This article uses novel daily data to understand what can be learned about the dynamics of consumer confidence and spending. In contrast to the existing literature that uses data collected at lower frequencies, I find that the estimated relationship between daily consumer confidence and daily spending is weak. I interpret this finding as an indication that on a day-to-day basis, consumers are rationally inattentive and do not react to small and temporary fluctuations in consumer confidence.