cssi/SciSciGPT-SciSciNet huggingface dataset:
Acute Toxicity of TFM and a TFM/Niclosamide Mixture to Selected Species of Fish, Including Lake Sturgeon (Acipenser fulvescens) and Mudpuppies (Necturus maculosus), in Laboratory and Field Exposures

The toxicity of the lampricides 3-trifluoromethyl-4-nitrophenol (TFM) and 2′,5-dichloro-4′-nitrosalicylanilide (niclosamide) to non-target fishes has been a major point of concern since their use to control larval sea lamprey (Petromyzon marinus) populations began in the early 1960s. The toxicity of TFM to several non-target fish species has been demonstrated in previous studies. However, little information is available on the toxicity of the TFM/1% niclosamide mixture. One species of particular concern is the lake sturgeon (Acipenser fulvescens). Juvenile lake sturgeon of several size ranges were exposed to determine potential effects of the lampricides to individuals present in treatment streams. Sac fry were most resistant to the lampricides followed by fingerlings in the 200 to 225 mm size range. Swim-up fry and fingerlings less than 100 mm were the most sensitive. Concentrations that produced 50% mortality (LC50 s) in juvenile lake sturgeon of these smaller size ranges were at or near the minimum lethal concentrations (MLCs) required for effective control of larval sea lampreys. The mudpuppy (Necturus maculosus), an amphibian native to several tributaries of the Great Lakes, have also become a species of interest in recent years. Laboratory tests conducted with TFM and a TFM/1% niclosamide mixture on adult mudpuppies indicate that although the amphibian is sensitive to the lampricides, an adequate margin of safety exists for adult mudpuppies to survive when exposed during stream treatments. Fifteen other fish species native to streams treated with lampricides were investigated in the laboratory to determine their sensitivity to the lampricides. Centrarchids, bluegill (Lepomis macrochirus) and green sunfish (Lepomis cyanellus) were the least sensitive to TFM, while ictalurids, black bullhead (Ictalurus melas), channel catfish (Ictalurus punctatus), and tadpole madtom (Notorus gyrinus) were the most sensitive. On-site bioassays conducted before lampricide treatments also revealed that lake sturgeon, channel catfish, and whitefish (Coregonus clupeaformis) were sensitive to the lampricides although considerably less sensitive compared to sea lamprey.
Intramolecular chiral communication in peptide–dendron hybrids

The conformational properties of a series of peptide–dendron hybrids progressively incorporating 1–4 dendritic side chains were investigated by circular dichroism. Although the presence of multiple adjacent dendrons along the peptide backbone precluded the formation of α-helical or β-sheet secondary structure, intramolecular packing of the dendrons mediated efficient peptide → dendron chirality transfer in both organic and aqueous media.
Genetic mouse models for understanding LRRK2 biology, pathology and pre-clinical application

Missense mutations in Leucine-Rich Repeat kinase 2 (LRRK2) are the most common cause of inherited Parkinson's disease (PD). Elucidation of LRRK2 biology and pathophysiology is central to the development of therapeutic intervention. Our group and others have developed a number of genetic mouse models of LRRK2 utilizing different genetic approaches. These models exhibit certain PD-related pathologies (e.g. impaired dopamine transmission and tauopathies) and abnormal motor functions, providing valuable insight into potential LRRK2-mediated pathogenesis of PD. However, not surprisingly they lack of substantial neuropathology and clinical syndromes of PD. Ongoing investigation of these models has begun to shed light on LRRK2 cellular functions and pathogenic pathways and is expected to assist the identification and validation of PD drug targets. This report summarizes the recent findings in our genetic LRRK2 models and discusses their utility in understanding much needed knowledge regarding early stage (pre-symptomatic) disease progression, drug target identification, and potential application in chemical screening focused on inhibitors of kinase activity of LRRK2.
Cofactor Regeneration of both NAD+ from NADH and NADP+ from NADPH:NADH Oxidase from Lactobacillus sanfranciscensis

A possible solution for the regeneration of NAD+ from NADH is the oxidation of NADH with concomitant reduction of oxygen catalyzed by NADH oxidase (E. C. 1.6.-.-). We employ NADH oxidase from Lactobacillus sanfranciscensis, which reduces O2 to innocuous H2O, and (R)-alcohol dehydrogenase [(R)-ADH] from Lactobacillus brevis to perform enantioselective oxidation of racemic phenylethanol to acetophenone and (S)-phenylethanol with regeneration of either NADH or NADPH to their respective oxidized precursors. NADH oxidase from L. sanfranciscensis accepts both NADH and NADPH; in contrast, the wild-type (R)-ADH only accepts NADP(+)(H) whereas its G37D mutant strongly prefers NAD(+)(H). Highly purified. NADH oxidase (221 U/mg, two-step protocol) was coupled with wild-type ADH from L. brevis on NADP(H) and mutant ADH from L. brevis on NAD(H) to achieve 50% conversion of racemic phenylethanol to (S)-phenylethanol and acetophenone. Depending on the relative concentration of alcohol to cofactor, up to more than 100 turnovers were observed. We believe that this is the first demonstration of a regeneration scheme for both NAD+ from NADH and NADP+ from NADPH with the same enzyme.
The role of computed tomography in the evaluation of postmastectomy locally recurrent breast cancer

Abstract The rate of post mastectomy local-regional recurrence of breast cancer has remained in the range of 10–30% for decades. The traditional treatment, external beam radiation therapy, is successful in eradicating local disease in most cases, but re-recurrences are seen in about 50% of patients. Since 1982, 33 patients with such recurrences have undergone evaluation with computed tomography (CT) at our institution as part of their diagnostic work-up. In 2233 (67%), CT revealed unsuspected disease, and in 10 of these patients the radiation treatment plan had to be altered. These results, similar to three other published series, strongly suggest that CT is a necessary part of the work-up of patients with post-mastectomy local-regional recurrences. The significance of these findings with respect to the cause of post mastectomy local-regional failures is further discussed.
Defining the transcriptome of accelerated and replicatively senescent keratinocytes reveals links to differentiation, interferon signaling, and Notch related pathways

Epidermal keratinocytes (KCs) undergo highly orchestrated morphological and molecular changes during transition from proliferative compartment into growth arrested early and late differentiation layers, prior to dying in outermost cornified layers of normal skin. Creation of stratum corneum is vital to barrier function protecting against infection. Transcriptional events in KCs regulating complex processes of differentiation and host defense required to maintain constant epidermal thickness and resistance to infection in either young or aged skin are largely unknown. Furthermore, as terminal differentiation is characterized by irreversible loss of replicative potential culminating in dead layers at the skin surface, this process may be viewed as a form of senescence. However, a complete transcriptional profile of senescent (SN) human KCs has not been previously defined to permit delineation of molecular boundaries involving differentiation and senescence. To fill this void, we utilized global transcriptional analysis of KCs maintained in vitro as either cultures of proliferating (PR) cells, early and late confluent (LC) (accelerated senescence) cultures, or KCs undergoing replicative senescence. Global gene expression profiling revealed early confluent (EC) KCs were somewhat similar to PR KCs, while prominent differences were evident when compared to LC KCs; which were also distinct from replicatively SN KCs. While confluent KCs have in common several genes regulating differentiation with replicatively SN KCs, the latter cells expressed elevated levels of genes involved in interferon signaling and inflammatory pathways. These results provide new insights into cell autonomous transcriptional-based programs operative within KCs contributing to replicative senescence, with partial sharing of genes involved in differentiation. In addition, regulation of KC senescence may involve participation of interferon signaling pathways derived from the important role of KCs in protecting skin from infection. Integrating all of the transcriptional data revealed a key role for Notch receptor mediated signaling in the confluency induced differentiation phenotype using this model system.
Asymptotic solutions for laminar dispersion in circular tubes

Abstract A short-time asymptotic solution is developed for the dispersion of a passive solute in the fully developed laminar flow of a Newtonian fluid through a straight circular tube. The new solution describes the axial dependence of the average concentration both near the leading and trailing edges of the concentration distribution, and it predicts the existence of a pronounced maximum near the trailing edge.
System identification-based lead-acid battery online monitoring system for electric vehicles

A system identification-based model for the online monitoring of batteries for electric vehicles (EVs) is presented. This algorithm uses a combination of battery voltage and current measurements plus battery data sheet information to implement model-based estimation of the stored energy, also referred to as state-of-charge (SOC), and power capability, also referred to as state-of-function (SOF), for deep-cycle batteries. This online monitoring scheme has been implemented for a bank of deep-cycle lead-acid batteries and experimental laboratory tests using simulated driving cycles have yielded promising results. In addition, actual road data from an EV powered by these same batteries has been analyzed with the proposed model to demonstrate the system's usefulness in determining the battery state-of-health (SOH). Finally, the limitation of the use of a linear model for battery terminal voltage behavior is discussed.
Diagnostic analysis of inventory systems: An optimization approach

The objective of a diagnostic analysis is to provide a measure of performance of an existing system and estimate the benefits of implementing a new one, if necessary. Firms expect diagnostic studies to be done promptly and inexpensively. Consequently, collection and manipulation of large quantities of data are prohibitive. In this paper we explore aggregate optimization models as tools for diagnostic analysis of inventory systems. We concentrate on the dynamic lot size problem with a family of items sharing the same setup, and on the management of perishable items. We provide upper and lower bounds on the total cost to be expected from the implementation of appropriate systems. However, the major thrust of the paper is to illustrate an approach to analyze inventory systems that could be expanded to cover a wide variety of applications. A fundamental by-product of the proposed diagnostic methodology is to identify the characteristics that items should share to be aggregated into a single family.
Circumferential variations of mechanical behavior of the porcine thoracic aorta during the inflation test

We developed an extension-inflation experimental apparatus with a stereo vision system and a stress– strain analysis method to determine the regional mechanical properties of a blood vessel. Seven proximal descending thoracic aortas were investigated during the inflation test at a fixed longitudinal stretch ratio of 1.35 over a transmural pressure range from 1.33 to 21.33 kPa. Four circumferential regions of each aorta were designated as the anterior (A), left lateral (L), posterior (P), and right lateral (R) regions, and the inflation test was repeated for each region of the aortas. We used continuous functions to approximate the surfaces of the regional aortic wall in the reference configuration and the deformed configuration. Circumferential stretch and stress at the four circumferential regions of the aorta were computed. Circumferential stiffness, defined as the tangent of the stress–stretch curve, and physiological aortic stiffness, named pressure–strain elastic modulus, were also computed for each region. In the low pressure range, the stress increased linearly with increased stretch, but the mechanical response became progressively stiffer in the high-pressure range above a transition point. At a transmural pressure of 12.00 kPa, mean values of stiffness were 4167 104 kPa (A), 523799 kPa (L), 6347 91 kPa (P), and 4897 82 kPa (R). The stiffness of the posterior region was significantly higher than that of the anterior region, but no significant difference was found in pressure–strain elastic modulus.
Edge states and the bulk-boundary correspondence in Dirac Hamiltonians

We present an analytic prescription for computing the edge dispersion $E(k)$ of a tight-binding Dirac Hamiltonian terminated at an abrupt crystalline edge. Specifically, we consider translationally invariant Dirac Hamiltonians with nearest-layer interaction. We present and prove a geometric formula that relates the existence of surface states as well as their energy dispersion to properties of the bulk Hamiltonian. We further prove the bulk-boundary correspondence between the Chern number and the chiral edge modes for quantum Hall systems within the class of Hamiltonians studied in the paper. Our results can be extended to the case of continuum theories that are quadratic in the momentum as well as other symmetry classes.
The effects of human leukemia inhibitory factor (hLIF) and culture medium on in vitro differentiation of cultured porcine inner cell mass (pICM).

Isolation and maintenance of porcine embryonic stem (pES) cells have been hindered by the inability to inhibit differentiation of the porcine inner cell mass (pICM) in vitro. Culture conditions currently in use have been developed from mouse ES cell culture and are not effective for maintaining the pICM. Optimizing culture conditions for the pICM is essential. We have developed a grading system to detect changes in the differentiation status of in vitro cultured pICM. Porcine ICMs (Day 7) were isolated by immunosurgery and cultured for 4 d in either Dulbecco’s modified Eagle’s medium (DMEM)-based medium (D medium) or DMEM/Ham’s F-10 (1:1)-based medium (D/H medium) with or without human Leukemia Inhibitory Factor (hLIF, 1000 iu/ml). Colonies were photographed daily for morphological analysis. pICMs were categorized into one of two types based on their morphological profile: type A, nonepithelial or type B, epithelial-like. Eight investigators evaluated pICM differentiation using standardized differentiation profiles. Each pICM series was graded on a scale of 1 (fully undifferentiated) to 5 (fully differentiated) for each time point. Differentiation was verified by alkaline phosphatase activity, cytokeratin staining, and scanning electron microscopy. Neither hLIF nor culture medium delayed differentiation of pICMs (P=0.08 and P=0.25, respectively). The grading system employed was an effective tool for detecting treatment effects on differentiation of the developing pICM. These results demonstrate that hLIF cannot significantly inhibit differentiation of the pICM, and is unlikely to assist in porcine ES cell isolation. Future experiments utilizing homologous cytokines may prove more beneficial.
Recruitment of Diverse Students in School Psychology Programs: Direction for Future Research and Practice

Lack of racially, ethnically, and linguistically (REL) diverse school psychologists has been a concern for decades. Recent and rapid increases in student diversity within America’s public schools require that school psychology address the longstanding lack of diversity within the field. This article details the demographics of school psychologists and school psychology graduate students. The article also examines the research related to recruitment of REL diverse individuals into the field. Based on an examination of the strengths and limitations of the existing body of knowledge, recommendations for future research and practice related to recruiting REL diverse individuals in school psychology graduate education are presented.
ASSIST-V: A tool for studying the implementation of operating systems

This paper describes a program called ASSIST-V which is designed to provide students and other researchers with an environment in which they may write and analyze operating systems for the IBM 360/370 series of machines. ASSIST-V is an extended version of a student-oriented assembler/interpreter called ASSIST. A user of ASSIST-V is presented with a machine which simulates the full set of S/360 machine instructions (including privileged instructions): the standard 360/370 interrupt structure, I/0 channels, and I/0 devices. Since the machine environment is simulated, ASSIST-V can provide debugging and statistics-gathering features not available on an actual “bare” machine.
Frequency-dependent polarizabilities of alkali-metal atoms from ultraviolet through infrared spectral regions

We present results of first-principles calculations of the frequency-dependent polarizabilities of all alkali-metal atoms for light in the wavelength range 300--1600 nm, with particular attention to wavelengths of common infrared lasers. We parametrize our results so that they can be extended accurately to arbitrary wavelengths above 800 nm. This work is motivated by recent experiments involving simultaneous optical trapping of two different alkali-metal species. Our data can be used to predict the oscillation frequencies of optically trapped atoms, and particularly the ratios of frequencies of different species held in the same trap. We identify wavelengths at which two different alkali-metal atoms have the same oscillation frequency.
The Impact of Germline BHD Mutation on Histological Concordance and Clinical Treatment of Patients With Bilateral Renal Masses and Known Unilateral Oncocytoma

Purpose: Managing oncocytoma in the setting of bilateral renal masses is a challenging scenario. Nevertheless, to our knowledge the pathological concordance of an oncocytic neoplasm in 1 kidney with tumors in the contralateral kidney is not known. We evaluated the influence of germline Birt-Hogg-Dube mutation on concordance rates to assist in managing these cases.Materials and Methods: We reviewed the records of patients at the National Institutes of Health between 1983 and 2009 who had bilateral renal masses, known pathology bilaterally and oncocytoma or an oncocytic neoplasm in at least 1 kidney. Oncocytoma or an oncocytic neoplasm in 2 renal units was considered concordant. Demographic, pathological and clinical data were collected.Results: The population consisted of 40 patients, including 23 with and 17 without a diagnosis of Birt-Hogg-Dube syndrome. Patients with the syndrome were younger (p <0.01) but there were no other differences between the 2 groups. However, patients with the syndrome had stat...
Osteonecrosis secondary to antiphospholipid syndrome: a case report, review of the literature, and treatment strategy

Osteonecrosis is commonly present in patients with antiphospholipid syndrome (APS) and systemic lupus erythematosus (SLE). Treatment of this condition remains extremely controversial. We present a treatment strategy of avascular necrosis of the knee in a patient with catastrophic antiphospholipid syndrome with a history of SLE and APS. Aggressive treatment with 12 rounds of plasmapheresis, intravenous immunoglobulin, rituximab, and cylophosphamide led to the patient’s recovery with no recurrence of symptoms during 16 months of follow up. In this report, we further discuss the pathogenesis of osteonecrosis and current understanding of the treatment of this disease.
Runtime repair of software faults using event-driven monitoring

In software with emergent properties, despite the best efforts to remove faults before execution, there is a high likelihood that faults will occur during runtime. These faults can lead to unacceptable program behavior during execution, even leading to the program terminating unexpectedly. Using a distributed event-driven runtime software-fault monitor to repair faulty states creates an enforceable runtime specification. Using such an architecture can help ensure that emergent systems operate within specification, increasing the reliability of such software.
A Corona-Charged Self-Biased Radiation Dosimeter

In this letter, we report on a new solid-state structure for an integrating microdosimeter. The device consists of a silicon bar enclosed by a compound dielectric bilayer of silicon dioxide/parylene acting as an ionization getter/electret. The dielectric bilayer is corona charged to establish a self-biasing structure that achieves high sensitivity without the need for a large applied voltage. For a 0.5 × 0.7 × 0.3 mm3 sensor with a dynamic range of 15 Gy, a sensitivity of 50 kΩ/Gy was measured.
A Cross-Cultural Study of Story Memory

This study examines some of the effects of culturally based knowledge on memory for stories about people performing common activities. Monocultural college students in (a) the United States and (b) Mexico read three brief stories about people going on a date, going home from the office for lunch, and starting a new semester of school. There were two versions of each story, consistent with either a U.S. or a Mexican cultural script. A modified recognition memory test for information in the stories occurred at one of three different delay intervals (immediately after reading the stories, 1/24 hour later, or 1 week later). Results showed that, after 1 week, both groups of subjects misremembered the stories from the other culture as being more like their own culture than they in fact were. The findings were consistent with previous monocultural research and argue that any theory of the effects of script-based knowledge on memory must consider the cultural origin of that knowledge. Problems associated with use...
A paradigmatic object-oriented programming language: Design, static typing and semantics†

To illuminate the fundamental concepts involved in object-oriented programming languages, we describe the design of TOOPL, a paradigmatic, statically-typed, functional, object-oriented programming language which supports classes, objects, methods, hidden instance variables, subtypes and inheritance.It has proven to be quite difficult to design such a language which has a secure type system. A particular problem with statically type checking object-oriented languages is designing typechecking rules which ensure that methods provided in a superclass will continue to be type correct when inherited in a subclass. The type-checking rules for TOOPL have this feature, enabling library suppliers to provide only the interfaces of classes with actual executable code, while still allowing users to safely create subclasses. To achieve greater expressibility while retaining type-safety, we choose to separate the inheritance and subtyping hierarchy in the language.The design of TOOPL has been guided by an analysis of the semantics of the language, which is given in terms of a model of the F-bounded second-order lambda calculus with fixed points at both the element and type level. This semantics supports the language design by providing a means to prove that the type-checking rules are sound, thus guaranteeing that the language is type-safe.While the semantics of our language is rather complex, involving fixed points at both the element and type level, we believe that this reflects the inherent complexity of the basic features of object-oriented programming languages. Particularly complex features include the implicit recursion inherent in the use of the keyword, self, to refer to the current object, and its corresponding type, MyType. The notions of subclass and inheritance introduce the greatest semantic complexities, whereas the notion of subtype is more straightforward to deal with. Our semantic investigations lead us to recommend caution in the use of inheritance, since small changes to method definitions in subclasses can result in major changes to the meanings of the other methods of the class.
Efficient fluoride-selective fluorescent host: experiment and theory.

A new naphthalene derivative containing a urea group at the 1,8-position of naphthalene was synthesized and showed a unique absorption and fluorescence peak with fluoride ion. Calculations suggested that a new peak was attributed to the increased anionic character of urea nitrogen due to the strongly charged hydrogen bonding between fluoride and amide protons of the urea. The fluoride selectivity among halides (F-, Cl-, Br-) comes from the fact that the fluoride approaches much closer to the amide protons than other halides and resides in the cavity with fast dynamics. The nature of electronic transitions that were analyzed from the calculations by the collective electronic oscillator method also supports the anionic nature of the complex between host and fluoride.
Piecewise linear cylinder models for 3-dimensional axon segmentation in Brainbow imagery

Generalized cylinder shapes are ubiquitous in biological systems and image processing techniques to identifies these tubular objects in 3D from biomedical imagery in various modalities is a general problem of interest. One such structure that exhibits branching tubular forms are neuronal networks; specifically, recent developments in microscopy imaging technology allow researchers to acquire massive amounts of 3D color images of neural structures that need to be tracked in 3D to extract structure for the purpose of studying function. In this paper, we propose a piecewise linear generalized cylinder tracing algorithm that exploits both edge and color information in order to automatically trace axons of neurons in Brainbow imagery. Results indicate that the proposed method can successfully trace multiple axons in dense neighborhoods.
Prediction of hygro—thermal stress distribution in proton exchange membranes using a three-dimensional multi-phase computational fluid dynamics model

AbstractA three-dimensional, multi-phase, non-isothermal computational fluid dynamics model of a proton exchange membrane fuel cell has been developed to simulate the hygro and thermal stresses in polymer membrane, which developed during the cell operation. The behaviour of the membrane during the operation of a unit cell has been studied and investigated. The model accounts for both gas and liquid phase in the same computational domain, and thus allows for the implementation of phase change inside the gas diffusion layers. The model includes the transport of gaseous species, liquid water, protons, energy, and water dissolved in the ion-conducting polymer. The new feature of the present model is to incorporate the effect of hygro and thermal stresses into actual three-dimensional, multi-phase, non-isothermal fuel cell model. In addition to hygro—thermal stresses, the model features an algorithm that allows for a more realistic representation of the local activation overpotentials, which leads to improved ...
What Did Unions Do in Nineteenth-Century Britain?

The article examines the development of the insurance function of trade unions. It analyzes how such policies worked, and why union benefit packages differed across occupations. It also addresses the impact of insurance policies on union organization. Insurance benefits increased the ability of unions to attract and retain members. They did not, however, significantly increase the power of union leaders relative to employers or union rank and file.
Computation of EM effects on large biological bodies by an iterative moment method

An iterative moment method algorithm using a conjugate gradient method is developed for a three-dimensional arbitrarily shaped dielectric or biological body. The algorithm has a restart feature which allows the operator to pause at a preset stage and then resume the iteration in a continuous way, thus making the computation of large bodies a controlled and measured process with minimum cost and time for a desired accuracy. It was observed that the effect of initial guess has only a small effect, while the direction vectors g/sup (0)/ and correction magnitude A/sup (0)/ are much more important in achieving a faster rate of convergence. >
Clinical review: Drug metabolism and nonrenal clearance in acute kidney injury

Decreased renal drug clearance is an obvious consequence of acute kidney injury (AKI). However, there is growing evidence to suggest that nonrenal drug clearance is also affected. Data derived from human and animal studies suggest that hepatic drug metabolism and transporter function are components of nonrenal clearance affected by AKI. Acute kidney injury may also impair the clearance of formed metabolites. The fact that AKI does not solely influence kidney function may have important implications for drug dosing, not only of renally eliminated drugs but also of those that are hepatically cleared. A review of the literature addressing the topic of drug metabolism and clearance alterations in AKI reveals that changes in nonrenal clearance are highly complicated and poorly studied, but they may be quite common. At present, our understanding of how AKI affects drug metabolism and nonrenal clearance is limited. However, based on the available evidence, clinicians should be cognizant that even hepatically eliminated drugs and formed drug metabolites may accumulate during AKI, and renal replacement therapy may affect nonrenal clearance as well as drug metabolite clearance.
Multi-drug resistance protein 4 (MRP4)-mediated regulation of fibroblast cell migration reflects a dichotomous role of intracellular cyclic nucleotides.

It has long been known that cyclic nucleotides and cyclic nucleotide-dependent signaling molecules control cell migration. However, the concept that it is not just the absence or presence of cyclic nucleotides, but a highly coordinated balance between these molecules that regulates cell migration, is new and revolutionary. In this study, we used multidrug resistance protein 4 (MRP4)-expressing cell lines and MRP4 knock-out mice as model systems and wound healing assays as the experimental system to explore this unique and emerging concept. MRP4, a member of a large family of ATP binding cassette transporter proteins, localizes to the plasma membrane and functions as a nucleotide efflux transporter and thus plays a role in the regulation of intracellular cyclic nucleotide levels. Here, we demonstrate that mouse embryonic fibroblasts (MEFs) isolated from Mrp4−/− mice have higher intracellular cyclic nucleotide levels and migrate faster compared with MEFs from Mrp4+/+ mice. Using FRET-based cAMP and cGMP sensors, we show that inhibition of MRP4 with MK571 increases both cAMP and cGMP levels, which results in increased migration. In contrast to these moderate increases in cAMP and cGMP levels seen in the absence of MRP4, a robust increase in cAMP levels was observed following treatment with forskolin and isobutylmethylxanthine, which decreases fibroblast migration. In response to externally added cell-permeant cyclic nucleotides (cpt-cAMP and cpt-cGMP), MEF migration appears to be biphasic. Altogether, our studies provide the first experimental evidence supporting the novel concept that balance between cyclic nucleotides is critical for cell migration. Background: MRP4 is an endogenous transporter of cyclic nucleotides that can regulate cell migration. The role of MRP4 in fibroblast migration is unknown. Results: MRP4-deficient fibroblasts migrate faster and have a moderately higher level of intracellular cyclic nucleotides. Conclusion: Inhibition of MRP4 increases fibroblast migration via alteration of intracellular cyclic nucleotide levels. Significance: Inhibition of MRP4 facilitates wound repair.
An Updated Classification of the Class Magnoliopsida (“Angiospermae”)

The present classification of extant flowering plants (Magnoliopsida) updates and revises those presented previously by bringing together the vast majority of new information published since 1999. The extant members of Magnoliopsida are subdivided into 12 subclasses, 35 superorders, 87 orders, 40 suborders, 472 families, and 400 subfamilies. The number of genera and species for each accepted taxon is indicated, along with a statement of the taxonomic confidence that the taxon is worthy of recognition as outlined. Authorships and year of publication are provided for each accepted name and cited synonym. A rule change adopted in 2005 at the Vienna Botanical Congress has fundamentally altered authorships and valid places of publication for hundreds of names, and the full extent of the changes is unknown at present. Newly proposed names include subclass Malvidae; superorder Berberidopsidanae and Huerteanae; suborders Aralidiineae, Asphodelineae, Chloranthineae, Eriocaulineae, Hamamelidineae, Juglandineae, Myricineae, Papaverineae, and Xyridineae; and subfamilies Circaeasteroideae, Dampieroideae, Dasypogonoideae, Davidsonioideae, Ebenoideae, Goetzeoideae, Hesperocallidoideae, Hortonioideae, Isophysidoideae, Kingdonioideae, Laxmannioideae, Ledocarpoideae, Lilaeoideae, Lomandroideae, Morkillioideae, Octoknemoideae, Petunioideae, Phormioideae, Rhynchothecoideae, Sargentodoxoideae, Sclerophylacoideae, Siparunoideae, Sparattanthelioideae, Styloceratoideae, Tribeloideae, Tricyrtidoideae, and Xanthoceroideae.
Local Scales of Turbulence in the Stable Boundary Layer

Local, gradient-based scales, which contain the vertical velocity and temperature variances, as well as the potential temperature gradient, but do not include fluxes, are tested using data collected during the CASES-99 experiment. The observations show that the scaling based on the temperature variance produces relatively smaller scatter of empirical points. The resulting dimensionless statistical moments approach constant values for sufficiently large values of the Richardson number Ri. This allows one to derive predictions for the Monin–Obukhov similarity functions φm and φh, the Prandtl number Pr and the flux Richardson number Rf in weak turbulence regime.
A note on the non‐geostrophic baroclinic stability problem

The baroclinic stability properties of non-geostrophic atmospheric flow in a channel are investigated. The model is formulated so as to include the effects of latitudinal variations of the perturbations and the basic state parameters, which constitute an essential aspect of the non-geostrophic baroclinic stability problem. Spectral and finite-difference techniques are applied to obtain numerical solutions and the results are found in agreement. In order to allow for high horizontal resolution the present calculations are restricted to a particular two-layer model which appears to incorporate the major non-geostrophic aspects of the baroclinic instability problem. A purely non-geostrophic type of baroclinic instability is found which bears some resemblance to barotropic instability in spite of the absence of latitudinal shear in the zonal flow. This instability is introduced by the twisting term in the vorticity equation and the non-geostrophic advection of temperature in the thermodynamic equation. For low Richardson numbers and short cyclonic waves this instability attains the same order of magnitude as the quasi-geostrophic baroclinic instability. The major instability found in the present model however is not different from the major quasi-geostrophic instability. DOI: 10.1111/j.2153-3490.1971.tb00550.x
Attention Alters Perceived Attractiveness

Can attention alter the impression of a face? Previous studies showed that attention modulates the appearance of lower-level visual features. For instance, attention can make a simple stimulus appear to have higher contrast than it actually does. We tested whether attention can also alter the perception of a higher-order property-namely, facial attractiveness. We asked participants to judge the relative attractiveness of two faces after summoning their attention to one of the faces using a briefly presented visual cue. Across trials, participants judged the attended face to be more attractive than the same face when it was unattended. This effect was not due to decision or response biases, but rather was due to changes in perceptual processing of the faces. These results show that attention alters perceived facial attractiveness, and broadly demonstrate that attention can influence higher-level perception and may affect people's initial impressions of one another.
Sustainable Building Design: A Challenge at the Intersection of Machine Learning and Design Optimization

Residential and commercial buildings are responsible for about 40% of primary energy consumption in the United States, hence improving their energy efficiency could have important sustainability benefits. The design of a building has tremendous effect on its energy profile, and recently there has been an increased interest in developing optimization methods that support the design of high performance buildings. Previous approaches are either based on simulation optimization or on training an accurate predictive model that is queried during the optimization. We propose a method that more tightly integrates the machine learning and optimization components, by employing active learning during optimization. In particular, we use a Gaussian Process (GP) model for the prediction and active learning and multi-objective genetic algorithm NSGA-II for the optimization. We develop a comprehensive and publicly available benchmark for building design optimization. We evaluate 5 machine learning approaches on our dataset, and show that the GP model is competitive, in addition to being well-suited for the active learning setting. We compare our optimization approach against the 2-stage approach and simulation optimization. Our results show that our approach produces solutions at the Pareto frontier compared to the other two approaches, while using only a fraction of the simulations and time.
Climate change-related temperature impacts on warm season heat mortality: a proof-of-concept methodology using BenMAP.

Climate change is anticipated to raise overall temperatures and is likely to increase heat-related human health morbidity and mortality risks. The objective of this work was to develop a proof-of-concept approach for estimating excess heat-related premature deaths in the continental United States resulting from potential changes in future temperature using the BenMAP model. In this approach we adapt the methods and tools that the US Environmental Protection Agency uses to assess air pollution health impacts by incorporating temperature modeling and heat mortality health impact functions. This new method demonstrates the ability to apply the existing temperature−health literature to quantify prospective changes in climate-sensitive heat-related mortality. We compared estimates of future temperature with and without climate change and applied heat−mortality health functions to estimate relative changes in heat-related premature mortality. Using the A1B emissions scenario, we applied the GISS-II global circu...
Seasonal thermogenesis and changes in body mass of masked shrews, Sorex cinereus

Masked shrews (Sorex cinereus) are the most widely distributed shrews in North America, occurring from Alaska and Canada south into the northern one-half of the United States. Favorable winter survivorship of S. cinereus is reported from the Appalachian Mountains of Pennsylvania. In the present study, I examined the role of seasonal changes in resting metabolic rate, nonshivering thermogenesis, thermal conductance, and body mass of live-trapped shrews as mechanisms that enhance overwinter survivorship. Shrews were captured monthly from April 1992 to April 1993 in southwestern Pennsylvania. Temperatures at the ground surface ranged from -7 to 22°C, in February and April, respectively, and snow occurred from November to March. Mean resting metabolic rate for shrews was highest in autumn (8.30 ml O 2 g -1 h -1 ) and lowest in spring (5.75 ml O 2 g -1 h -1 ). Body temperature of shrews averaged 38.7°C. Thermal conductance averaged 0.79, ranging from a low in spring to a high in autumn. Mean nonshivering thermogenesis was lowest in summer (8.02 ml O 2 g -1 h -1 ), and peaked in winter (14.60 ml O 2 g -1 h -1 ). Capacity for nonshivering heat production in winter was almost twice that of summer. Nonshivering thermogenesis showed an inverse relationship to minimum ambient, ground surface, and subsurface temperatures. Body mass averaged 4.15 g and declined 53% from summer to winter. The favorable overwinter survivorship of S. cinereus is due in part to the ability of this species to increase thermogenic capacity by means of nonshivering thermogenesis coupled with energy conservation in the form of a decline in mass during winter.
Who Says “It’s the Economy”? Cross-National and Cross-Individual Variation in the Salience of Economic Performance:

Theories of government approval usually assume that voters care about economic outcomes. This assumption frequently does not hold. Data from the Comparative Study of Electoral Systems demonstrate that although the economy is often the most important issue in an election, its place on the issue agenda varies across individuals and electoral contexts. The economy is more likely to dominate other issue concerns under conditions of economic recession, volatility, and economic underdevelopment. Moreover, at the individual level the salience of economic performance rises with unemployment and economic vulnerability. Governance crises related to corruption and human rights reduce attention to the economy, as do large-scale terrorist attacks. If the economy is not perceived as important, its effect on government approval is strongly mitigated. Thus, variations in the economy’s salience need to be further incorporated into studies linking economic and political outcomes.
Influences of vacancy defects on buckling behaviors of open-tip carbon nanocones

This study investigated influences of vacancy defects on buckling behaviors of open-tip carbon nanocones (CNCs) by molecular dynamics simulations. Effects of vacancy location and temperature on the buckling behaviors were examined in the study. Some interesting findings were attained from the investigations. It was noticed that the CNC with an upper vacancy has comparable degradation in the critical strain and in the critical load with the CNC with a middle vacancy, whereas the CNC with a lower vacancy has lower degradation in the antibuckling ability than the above two CNCs. The antibuckling ability of the CNCs reduces with the growth of the temperature. This temperature effect is more apparent in the perfect CNC than in the vacancy-defect CNCs. It was also observed that the degradation in the antibuckling ability is obvious at a lower temperature, but it decreases as the temperature grows. Besides, all the CNCs (including the perfect and the vacancy-defect CNCs) exhibited a shrinking/swelling buckling mode shape at the studied temperatures. Existence of the vacancies did not alter the buckling mode shape of the CNCs.
The State of the (European) Union: From the Single Market to Maastricht, from Singular Events to General Theories

This review article examines four recent volumes on the European Union, each of which takes as its substantive and theoretical starting point the relaunching of the European Community in the mid-1980s around the single market initiative. Taken together, they provide a comprehensive account of the momentous events leading up to the Maastricht summit. They also present an accurate reflection of the current state of the subfield. Their basic research agenda, a continuation of traditional approaches in Community studies, revolves around the “big bangs” of integration and the conventional models of neofunctionalism and intergovemmentalism. This scholarly continuity generates unwelcome consequences for the selection of research puzzles and for the robustness and reach of the findings. As a remedy, several strategies—some methodological, others theoretical—are outlined for generating new insights into the growing complexity of the European Union.
Emerging Genetic Counselor Roles within the Biotechnology and Pharmaceutical Industries: as Industry Interest Grows in Rare Genetic Disorders, How are Genetic Counselors Joining the Discussion?

Traditionally, the biotechnology and pharmaceutical industry (BPI) has focused drug development at the mass-market level targeting common medical issues. However, a recent trend is the development of therapies for orphan or rare disorders, including many genetic disorders. Developing treatments for genetic disorders requires an understanding of the needs of the community and translating genomic information to clinical and non-clinical audiences. The core skills of genetic counselors (GCs) include a deep knowledge of genetics and ability to communicate complex information to a broad audience, making GCs a choice fit for this shift in drug development. To date there is limited data defining the roles GCs hold within this industry. This exploratory study aimed to define the roles and motivation of GCs working in BPI, assess job satisfaction, and identify translatable skills and current gaps in GC training programs. The authors surveyed 26 GCs working in BPI in the United States; 79 % work for companies focused on rare disorders. GC positions in BPI are growing, with 57 % of respondents being the first GC in their role. GCs in BPI continue to utilize core genetic counseling competencies, though 72 % felt their training did not fully prepare them for BPI. These data suggest opportunities for exposure to BPI in GC training to better prepare future generations of GCs for these career opportunities. GC satisfaction was high in BPI, notably in areas traditionally reported as less satisfying on the National Society for Genetic Counselors Professional Status Survey: salary and advancement opportunities. BPI’s growing interest in rare disorders represents a career opportunity for GCs, addressing both historic areas of dissatisfaction for GCs and BPI’s genomic communication needs.
Patterning by Photochemically Directing the Marangoni Effect

Polystyrene (PS) that has been exposed to ultraviolet light (UV) undergoes partial dehydrogenation of the alkane polymer backbone which increases its surface energy. Exploiting this photochemistry, we exposed polystyrene films to UV light using a photomask to induce a patterned photochemical reaction producing regions in the film with differing surface energy. Upon heating the solid polymer film with the preprogrammed surface energy pattern to a liquid state, the polymer flows from the low surface energy unexposed regions to high surface energy exposed regions. This flow creates three-dimensional topography by the Marangoni Effect, which describes convective mass transfer due to surface energy gradients. The topographical features can be permanently preserved by quenching the film below its glass to liquid transition temperature. Their shape and organization are only limited by the pattern on the photomask.
Comparative effects of 4-phenyl-3-butenoic acid and vorinostat on cell growth and signaling.

Background/Aim 4-phenyl-3-butenoic acid (PBA) is a small molecule anti-inflammatory agent which has been shown to inhibit growth, increase gap junction intercellular communication, and modulate activation of p38 mitogen-activated protein kinase (p38 MAPK) and c-jun n-terminal kinase (JNK) in tumorigenic cells at concentrations that do not similarly affect non-tumorigenic cells. Vorinostat is an anticancer agent structurally similar to PBA. The purpose of this study was to compare the effects of these two agents on JNK and p38 activation, cell growth and gap junction intercellular communication (GJIC).
Comment: The Interaction Between Metaphor and Emotion Processing in the Brain

It has been argued that metaphor and emotion processing are tightly linked together. Here we explore whether neuroscientific evidence supports this claim.