cssi/SciSciGPT-SciSciNet huggingface dataset:
Pulmonary Vein Antral Isolation and Nonpulmonary Vein Trigger Ablation Are Sufficient to Achieve Favorable Long-Term Outcomes Including Transformation to Paroxysmal Arrhythmias in Patients With Persistent and Long-Standing Persistent Atrial Fibrillation.

Background— Transformation from persistent to paroxysmal atrial fibrillation (AF) after ablation suggests modification of the underlying substrate. We examined the nature of initial arrhythmia recurrence in patients with nonparoxysmal AF undergoing antral pulmonary vein isolation and nonpulmonary vein trigger ablation and correlated recurrence type with long-term ablation efficacy after the last procedure. Methods and Results— Three hundred and seventeen consecutive patients with persistent (n=200) and long-standing persistent (n=117) AF undergoing first ablation were included. AF recurrence was defined as early (≤6 weeks) or late (>6 weeks after ablation) and paroxysmal (either spontaneous conversion or treated with cardioversion ≤7 days) or persistent (lasting >7 days). During median follow-up of 29.8 (interquartile range: 14.8–49.9) months, 221 patients had ≥1 recurrence. Initial recurrence was paroxysmal in 169 patients (76%) and persistent in 52 patients (24%). Patients experiencing paroxysmal (versus persistent) initial recurrence were more likely to achieve long-term freedom off antiarrhythmic drugs (hazard ratio, 2.2; 95% confidence interval, 1.5–3.2; P <0.0001), freedom on/off antiarrhythmic drugs (hazard ratio, 2.5; 95% confidence interval, 1.6–3.8; P <0.0001), and arrhythmia control (hazard ratio, 5.2; 95% confidence interval, 2.9–9.2; P <0.0001) after last ablation. Conclusions— In patients with persistent and long-standing persistent AF, limited ablation targeting pulmonary veins and documented nonpulmonary vein triggers improves the maintenance of sinus rhythm and reverses disease progression. Transformation to paroxysmal AF after initial ablation may be a step toward long-term freedom from recurrent arrhythmia.
Immunosenescence-Related Transcriptomic and Immunologic Changes in Older Individuals Following Influenza Vaccination.

The goal of annual influenza vaccination is to reduce mortality and morbidity associated with this disease through the generation of protective immune responses. The objective of the current study was to examine markers of immunosenescence and identify immunosenescence-related differences in gene expression, gene regulation, cytokine secretion, and immunologic changes in an older study population receiving seasonal influenza A/H1N1 vaccination. Surprisingly, prior studies in this cohort revealed weak correlations between immunosenescence markers and humoral immune response to vaccination. In this report we further examined the relationship of each immunosenescence marker (age, T cell receptor excision circle frequency, telomerase expression, percentage of CD28- CD4+ T cells, percentage of CD28- CD8+ T cells, and the CD4/CD8 T cell ratio) with additional markers of immune response (serum cytokine and chemokine expression) and measures of gene expression and/or regulation. Many of the immunosenescence markers indeed correlated with distinct sets of individual DNA methylation sites, miRNA expression levels, mRNA expression levels, serum cytokines, and leukocyte subsets. However, when the individual immunosenescence markers were grouped by pathways or functional terms, several shared biological functions were identified: antigen processing and presentation pathways, MAPK, mTOR, TCR, BCR, and calcium signaling pathways, as well as key cellular metabolic, proliferation and survival activities. Furthermore, the percent of CD4+ and/or CD8+ T cells lacking CD28 expression also correlated with miRNAs regulating clusters of genes known to be involved in viral infection. Integrated (DNA methylation, mRNA, miRNA, and protein levels) network biology analysis of immunosenescence-related pathways and genesets identified both known pathways (e.g., chemokine signaling, CTL and NK cell activity), as well as a gene expression module not previously annotated with a known function. These results may improve our ability to predict immune responses to influenza and aid in new vaccine development, and highlight the need for additional studies to better define and characterize immunosenescence.
Virtual Teach-To-Goal™ Adaptive Learning of Inhaler Technique for Inpatients with Asthma or COPD

Background Asthma and chronic obstructive pulmonary disease (COPD) result in more than 1 million hospitalizations annually. Most hospitalized patients misuse respiratory inhalers. This misuse can be corrected with in-person education; however, this strategy is resource intensive and skills wane quickly after discharge. Objective The objective of this study was to develop and pilot a virtual teach-to-goal™ (V-TTG™) inhaler skill training module, using innovative adaptive learning technology. Methods Eligible adults with asthma or COPD completed a V-TTG™ metered-dose inhaler session with tailored rounds of narrated demonstration and self-assessments. The primary outcome was the proportion of participants with inhaler misuse post- versus pre-V-TTG™; secondary analyses tested mastery, self-efficacy, and perceived versus actual inhaler skills. Analyses were tested with McNemar's χ 2 test ( P Results Among 90 enrolled participants, the majority were African American (94%), female (62%), and had asthma (68%), with a mean age of 48 years. Among those completing both pre- and post-V-TTG™ (n = 83), misuse was significantly lower post- versus pre-V-TTG™ (24% vs 83%, P P P P Conclusions This study is the first to demonstrate the efficacy of adaptive V-TTG™ learning to teach the inhaler technique. V-TTG™ improved most participants' technique to an acceptable level, reached mastery for half, and also increased self-efficacy and actualized skill. V-TTG™ has potential to improve health care across care transitions.
Wind Energy Conversion by Plant-Inspired Designs.

In 2008 the U.S. Department of Energy set a target of 20% wind energy by 2030. To date, induction-based turbines form the mainstay of this effort, but turbines are noisy, perceived as unattractive, a potential hazard to bats and birds, and their height hampers deployment in residential settings. Several groups have proposed that artificial plants containing piezoelectric elements may harvest wind energy sufficient to contribute to a carbon-neutral energy economy. Here we measured energy conversion by cottonwood-inspired piezoelectric leaves, and by a “vertical flapping stalk”—the most efficient piezo-leaf previously reported. We emulated cottonwood for its unusually ordered, periodic flutter, properties conducive to piezo excitation. Integrated over 0°–90° (azimuthal) of incident airflow, cottonwood mimics outperformed the vertical flapping stalk, but they produced << daW per conceptualized tree. In contrast, a modest-sized cottonwood tree may dissipate ~ 80 W via leaf motion alone. A major limitation of piezo-transduction is charge generation, which scales with capacitance (area). We thus tested a rudimentary, cattail-inspired leaf with stacked elements wired in parallel. Power increased systematically with capacitance as expected, but extrapolation to acre-sized assemblages predicts << daW. Although our results suggest that present piezoelectric materials will not harvest mid-range power from botanic mimics of convenient size, recent developments in electrostriction and triboelectric systems may offer more fertile ground to further explore this concept.
Relationships between self-determination theory and theory of planned behavior applied to physical activity and exercise behavior in chronic pain

AbstractThis study examined the relationships between self-determination theory (SDT) and theory of planned behavior (TpB) applied to physical activity and exercise behavior (PA&E) in people with chronic pain. Two hundred and eleven adults with chronic musculoskeletal pain (28 males and 183 females, age range 18 to 82 years, mean age 43 years) were recruited from online support groups and clinic networks in the United States. Participants completed SDT measures relevant to PA&E on perceived autonomy support, autonomy, competence, and relatedness, as well as TpB measures relevant to PA&E on intention, attitudes, subjective norms, and perceived behavioral control. Correlational techniques and canonical correlation analysis were performed to examine the relationships and variance within and between theoretical dimensions. Overall, the SDT set accounted for 37% of the TpB variance and the TpB set accounted for 32% of the SDT set variance. The results indicate there are statistical similarities and differences...
Comparison of first trimester transvaginal anatomy scan to the second trimester structural survey in normal weight and obese populations: a pilot study

AbstractPurpose: To compare visualization of first trimester fetal anatomic transvaginal ultrasound (TVUS) to the second trimester transabdominal anatomic ultrasound (TAU) in normal weight and obese patients.Materials and methods: In a prospective cross-sectional study design, 25 women underwent a TVUS between 12 and 14 weeks, and a TAU at 18–22 weeks. For each anatomic structure, the percentage of patients achieving optimal visualization was recorded. Risk ratios for visualizing a structure in the second trimester versus the first were calculated.Results: Twenty-five patients underwent a TVUS and 24 completed the TAU. The average BMI in the obese and nonobese groups was 34 kg/m2 and 23 kg/m2, respectively. All structures were more consistently visualized during the TAU for the both groups. The cardiac views, hands and feet were more difficult to visualize in all the patients at both scan times but were more frequently visualized in the TAU. For the obese patients, hands, feet, cardiac and spine views wer...
Chronic kidney disease: KDIGO CKD-MBD guideline update: evolution in the face of uncertainty.

Management of mineral and bone disorders in patients with chronic kidney disease (CKD–MBD) requires an understanding of the complex interactions among ions, hormones and their target organs. Since publication of the KDIGO CKD–MBD guideline in 2009, our understanding of disease pathophysiology has improved; however, a paucity of high-quality clinical evidence to support specific interventions remains. Using available data, KDIGO has now updated diagnostic and therapeutic recommendations for patients with CKD–MBD.
The ratio of circulating regulatory cluster of differentiation 4 T cells to endothelial progenitor cells predicts clinically significant acute rejection after heart transplantation.

Background The aim of this study was to determine the value of the ratio of the percentage of circulating regulatory cluster of differentiation 4 T cells (%Tregs) to the percentage of endothelial progenitor cells (%EPCs; Treg/EPC ratio) for predicting clinically significant acute rejection. Methods Peripheral blood %Tregs and %EPCs were quantified in 91 cardiac transplant recipients using flow cytometry at a mean of 42 ± 13 days after transplant. The primary end point was clinically significant acute rejection, defined as an event that led to an acute augmentation of immunosuppression in conjunction with an International Society for Heart and Lung Transplantation grade ≥ 2R in a right ventricular endomyocardial biopsy specimen or non-cellular rejection (specimen-negative rejection) with hemodynamic compromise (decrease in left ventricular ejection fraction by > 25%). Results Significant rejection occurred in 27 recipients (29.7%) during a median of 49.4 months (interquartile range, 37.0–62.0 months). The mean %Tregs and %EPCs were not significantly different between those with and without an episode of significant rejection, but the mean Treg/EPC ratio was significantly lower in recipients with significant rejection (44.9 vs 106.7, p = 0.001). Receiver operating characteristic curve analysis showed an area under the curve value for significant rejection for a Treg/EPC ratio of 0.712. The best cutoff value of the Treg/EPC ratio that distinguished between those with or without significant rejection was ≤ 18 by receiver operating characteristic curve analysis. Kaplan-Meier analysis revealed that patients with a Treg/EPC ratio of ≤ 18 had a significantly higher rate of rejection than those with a Treg/EPC ratio > 18 (61.5% vs 16.9%, log-rank p Conclusions A low Treg/EPC ratio measured soon after heart transplantation is an independent predictor of acute rejection. The Treg/EPC ratio has potential as an early biomarker after heart transplantation for predicting acute rejection.
Mycobacterium abscessus Smooth and Rough Morphotypes Form Antimicrobial-Tolerant Biofilm Phenotypes but Are Killed by Acetic Acid.

Mycobacterium abscessus has emerged as an important pathogen in people with chronic inflammatory lung diseases such as cystic fibrosis, and recent reports suggest that it may be transmissible by fomites. M. abscessus exhibits two major colony morphology variants: a smooth morphotype (MaSm ) and a rough morphotype (MaRg ). Biofilm formation, prolonged intracellular survival, and colony variant diversity can each contribute to the persistence of M. abscessus and other bacterial pathogens in chronic pulmonary diseases. A prevailing paradigm of chronic M. abscessus infection is that MaSm is a noninvasive, biofilm-forming, persistent phenotype and MaRg an invasive phenotype that is unable to form biofilms. We show that MaRg is hyperaggregative and forms biofilm-like aggregates, which, like MaSm biofilm aggregates, are significantly more tolerant than planktonic variants to acidic pHs, hydrogen peroxide (H2O2), and treatment with amikacin or azithromycin. We further show that both variants are recalcitrant to antibiotic treatment inside human macrophage-like cells and that MaRg is more refractory than MaSm to azithromycin. Our results indicate that biofilm-like aggregation and protracted intracellular survival may each contribute to the persistence of this problematic pathogen in the face of antimicrobial agents regardless of morphotype. Biofilms of each M. abscessus variant are rapidly killed, however, by acetic acid, which may help to prevent local fomite transmission.
Monitoring of Dung Beetle (Scarabaeidae and Geotrupidae) Activity Along Maryland's Coastal Plain

Our understanding of how human activities impact insect communities is limited. Dung beetles, well known for the ecosystem services they provide, are faced with many conservation threats, particularly from deforestation and agriculture. Here we used 200-m transects and human-dung—baited pitfall traps to examine dung beetle populations in 7 forests of Maryland's Coastal Plain. We set traps once a month, from May 2014 to April 2015, to determine species presence, abundance, range, and seasonality. We collected 6463 individuals representing 22 species; Janes Island State Park (JISP) had the highest abundance (2705 individuals) and Martinak State Park (MSP) had the highest species richness (19 species). During summer 2015, we examined the succession of dung beetles attracted to bait in JISP and MSP. We set 10 traps once a month (May–August) in each site and collected beetles on days 1, 3, 5, 7, 14, and 21 without dung replacement. In JISP, Onthophagus hecate (Scooped Scarab) was abundant throughout each 21-d period, and accounted for 68% of all beetles collected. In MSP, most specimens were collected by day 5. Here we provide information for conservation of locally rare or uncommon species.
High-Risk Prescription Opioid Use Among People Living With HIV.

Background Prescription opioid use is greater among people living with HIV (PLWH), yet little is known about the prevalence of specific types of high-risk use among these individuals. Setting We analyzed clinical and demographic data from the HIV Research Network and prescribing data from Medicaid for noncancer patients seeking HIV treatment at 4 urban clinics between 2006 and 2010. Methods HIV Research Network patients were included in the analytic sample if they received at least one incident opioid prescription. We examined 4 measures of high-risk opioid use: (1) high daily dosage; (2) early refills; (3) overlapping prescriptions; and (4) multiple prescribers. Results Of 4605 eligible PLWH, 1814 (39.4%) received at least one incident opioid prescription during follow-up. The sample was 61% men and 62% African American with a median age of 44.5 years. High-risk opioid use occurred among 30% of incident opioid users (high daily dosage: 7.9%; early refills: 15.9%; overlapping prescriptions: 16.4%; and multiple prescribers: 19.7%). About half of the cumulative incidence of high-risk use occurred within 1 year of receiving an opioid prescription. After adjusting for study site, high-risk opioid use was greater among patients with injection drug use as an HIV risk factor [adjusted hazard ratio (aHR) = 1.39, 95% confidence interval: 1.11 to 1.74], non-Hispanic whites [aHR = 1.61, (1.21 to 2.14)], patients age 35-45 [aHR = 1.94, (1.33 to 2.80)] and 45-55 [aHR = 1.84, (1.27 to 2.67)], and patients with a diagnosis of chronic pain [aHR = 1.32, (1.03 to 1.70)]. Conclusions A large proportion of PLWH received opioid prescriptions, and among these opioid recipients, high-risk opioid use was common. High-risk use patterns often occurred within the first year, suggesting this is a critical time for intervention.
Occipital neuralgia associates with high cervical spinal cord lesions in idiopathic inflammatory demyelinating disease.

BackgroundThe association of trigeminal neuralgia with pontine lesions has been well documented in multiple sclerosis, and we tested the hypothesis that occipital neuralgia in multiple sclerosis is...
Post-traumatic stress symptoms are associated with better performance on a delayed match-to-position task

Many individuals with posttraumatic stress disorder (PTSD) report experiencing frequent intrusive memories of the original traumatic event (e.g., flashbacks). These memories can be triggered by situations or stimuli that reflect aspects of the trauma and may reflect basic processes in learning and memory, such as generalization. It is possible that, through increased generalization, non-threatening stimuli that once evoked normal memories become associated with traumatic memories. Previous research has reported increased generalization in PTSD, but the role of visual discrimination processes has not been examined. To investigate visual discrimination in PTSD, 143 participants (Veterans and civilians) self-assessed for symptom severity were grouped according to the presence of severe PTSD symptoms (PTSS) vs. few/no symptoms (noPTSS). Participants were given a visual match-to-sample pattern separation task that varied trials by spatial separation (Low, Medium, High) and temporal delays (5, 10, 20, 30 s). Unexpectedly, the PTSS group demonstrated better discrimination performance than the noPTSS group at the most difficult spatial trials (Low spatial separation). Further assessment of accuracy and reaction time using diffusion drift modeling indicated that the better performance by the PTSS group on the hardest trials was not explained by slower reaction times, but rather a faster accumulation of evidence during decision making in conjunction with a reduced threshold, indicating a tendency in the PTSS group to decide quickly rather than waiting for additional evidence to support the decision. This result supports the need for future studies examining the precise role of discrimination and generalization in PTSD, and how these cognitive processes might contribute to expression and maintenance of PTSD symptoms.
Learning by Blind Students through Active and Passive Listening.

Studies were conducted comparing learning achieved by blind students at different grade levels for 3 types of material presented at normal and compressed rates under conditions of active and passiv...
Chapter 6 – Thin-Layer Chromatography

Thin-layer chromatography (TLC) traces its origins to the introduction of drop chromatography in the late 1930s. A microscope slide is covered with a layer of aluminum oxide, on which one drop of extract is spotted, followed by drop-wise addition of solvent onto this spot. Separated substances are visualized as circular zones radiating from the original spot center. For analytical applications, drop chromatography offers a faster, more convenient, and more powerful separation tool than conventional column chromatography. Devised standardized procedures are used to improve the separation performance and reproducibility of thin-layer chromatography, paving the way for its commercialization, as well as contributing many new applications. The fine particle layers and associated instrumentation were introduced in 1970s for their correct use. In this form, thin-layer chromatography became known as high-performance TLC, instrumental TLC, or modem TLC to distinguish it from its parent, now generally referred to as conventional TLC. Modern TLC did not displace conventional TLC from laboratory studies and the two approaches coexist today because of their complementary features. Conventional thin-layer chromatography provides a quick, inexpensive, flexible, and portable method for monitoring synthetic reactions and similar applications.
Interoperability via Enterprise Architecture.

This paper summarizes the latest research in interoperability and enterprise architecture. It evaluates their features with respect to their applicability in a wide range of domains. It also recommends a seven layer Reference Model for a distributive, object oriented, domain specific, data-centric, bottom-up design. It favors a spiral model for life cycle in heterogeneous environment enterprise architecture in order to enhance interoperability. Simple interoperability model by software engineering institute, joint technical architecture by Department of Defense, federal Reference Model by EA shared interest group and datadriven architect techniques are modified and used as the basis for this model.
Air combat situation assessment for UAV based on improved decision tree

Air combat situation assessment is a multi-parameter, nonlinear and real-time problem so that approach based on a novel structure of decision tree is proposed. Unlike traditional assessment method, information of both unmanned aerial vehicles (UAV) and enemy is focused as input to raise degree of assessment accuracy. Firstly, four attributes are established, which are foundations to evaluate situation from different aspect. Then, the principle of inference in SADT is explained in detail and air combat situation assessment decision tree (SADT) is structured. Lastly, extensive simulations, including one-to- one and two-to-one, are implemented and the results show that the proposed optimal method has better performance than traditional bayesian network.
Racial disparities and temporal trends in dementia misdiagnosis risk in the United States.

Abstract Introduction Systematic disparities in misdiagnosis of dementia across racial/ethnic groups have implications for health disparities. We compared the risk of dementia under- and overdiagnosis in clinical settings across racial/ethnic groups from 2000 to 2010. Methods We linked fee-for-service Medicare claims to participants aged ≥70 from the nationally representative Health and Retirement Study. We classified dementia status using an algorithm with similar sensitivity and specificity across racial/ethnic groups and assigned clinical dementia diagnosis status using ICD-9-CM codes from Medicare claims. Multinomial logit models were used to estimate relative risks of clinical under- and overdiagnosis between groups and over time. Results Non-Hispanic blacks had roughly double the risk of underdiagnosis as non-Hispanic whites. While primary analyses suggested a shrinking disparity over time, this was not robust to sensitivity analyses or adjustment for covariates. Risk of overdiagnosis increased over time in both groups. Discussion Our results suggest that efforts to reduce racial disparities in underdiagnosis are warranted.
Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform

Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic alphacoronavirus PED virus (PEDV). In the absence of effective vaccines, infection prevention and control through diagnostic testing and quarantine are critical. Early detection and differential diagnosis of PEDV infections increase the chance of successful control of the disease. Therefore, there is a continuous need for development of reduced assay-step protocols, no-wash, high-throughput immunoassays. This study described the characterization of the humoral immune response against PEDV under experimental and field conditions using a rapid, sensitive, luminescent proximity homogenous assay (AlphaLISA). PEDV IgG and IgA antibodies were developed toward the beginning of the second week of infection. PEDV IgG antibodies were detected for at least 16 weeks post-exposure. Remarkably, the serum IgA levels remained high and relatively stable throughout the study, lasting longer than the serum IgG response. Overall, AlphaLISA allows the detection and characterization of pathogen-specific antibodies with new speed, sensitivity, and simplicity of use. Particularly, the bridge assay constitutes a rapid diagnostic that substantially improves upon the "time to result" metric of currently available immunoassays.
Leadership in Surgery

Strong surgical leadership is required for Surgery to remain relevant to the future practice of medicine. Failing that, Surgery may be reduced to a technical specialty like interventional radiology, and vulnerable to loss of identity and to displacement from decision making regarding delivery of care. Surgical leadership must be future-oriented, applying lessons from the past to circumstances yet to come. At its best, leadership involves creation of a positive future by communicating the idea that cooperative behavior always achieves more than self-motivated behavior. This chapter serves to discuss the concept of leadership in surgery, values that are important, the leadership phenotype, and how to prepare for leadership in surgery.
Machine Learning for Structural Materials

The development of structural materials with outstanding mechanical response has long been sought for innumerable industrial, technological, and even biomedical applications. However, these compoun...
Kèlání and the Question of Sovereign Cinema

One of the great philosophical questions at the heart of Tunde Kelani's oeuvre concerns the sovereignty of cinematic practice. Can cinema, particularly in postcolonial Nigeria, be free from undue o...
Shaping urban futures in Mongolia: Ulaanbaatar, dynamic ownership and economic flux: by Rebekah Plueckhahn, London, UCL Press, 2020, 171 pp., £ 40.00 (hardback), ISBN 9781787351547; £ 20.000 (paperback), ISBN 9781787351530; free (Open Access PDF), ISBN 9781787351523

The burgeoning literature on urbanizing post-Socialist Asia vividly shows that navigating the terrain of urban land and home ownership is the substance of politics at every scale (Hsing 2010; Roy a...
Ten‐year survival after pathologic complete response in rectal adenocarcinoma

Background Multimodal treatment is the standard of care for rectal adenocarcinoma, with a subset of patients achieving a pathologic complete response (pCR). While pCR is associated with improved overall survival (OS), long-term data on patients with pCR is limited. Methods This is a single institution retrospective cohort study of all patients with clinical stages II/III rectal adenocarcinoma who underwent neoadjuvant chemoradiation therapy and operative resection (January 1, 2004-December 31, 2017). PCR was defined as no tumor identified in the rectum or associated lymph nodes by final pathology. Results Of 370 patients in this cohort, 50 had a pCR (13.5%). For pCR patients, 5-year disease-free survival (DFS) was 92%, 5-year OS was 95%. Twenty-six patients had surgery > 10 years before the study end date, of which 20 had an OS > 10 years (77%) with median OS 12.1 years and 95% alive to date (19/20). Of the 50 pCR patients, there was a single recurrence in the lung at 44.3 months after proctectomy which was surgically resected. Conclusion For patients with rectal adenocarcinoma that undergo neoadjuvant chemoradiation and surgical resection, pCR is associated with excellent long-term DFS and OS. Many patients live greater than 10 years with no evidence of disease recurrence.
Stay-at-Home Orders during the COVID-19 Pandemic Reduced Urban Water Use

In response to the outbreak of the COVID-19 pandemic, many governments instituted “stay-at-home” orders to prevent the spread of the coronavirus. The resulting changes in work and life routines had the potential to substantially perturb typical patterns of urban water use. We present here an analysis of how these pandemic responses affected California’s urban water consumption. Using water demand modeling that fuses an integrated water use database, we first simulated the water use in a business-as-usual (non-pandemic) scenario for essentially all urban areas in California. We then subtracted the business-as-usual water use from the actual use to isolate the changes caused solely by the pandemic response. We found that the pandemic response decreased California’s urban water use by 7.9%, which can be largely attributed to an 11.2% decrease in the commercial, industrial, and institutional sector that more than offset a 1.4% increase in the residential sector. The influence of the stay-at-home practices on urban water use is slightly stronger than the combined influences of all non-pandemic factors. This study covers both metropolitans and suburbs; therefore, the results could also be useful for analysis of the impacts of COVID-19 on water use in other urban areas.
Posterior Polyethylene Tethers Reduce Occurrence of Proximal Junctional Kyphosis After Multilevel Spinal Instrumentation for Adult Spinal Deformity: A Retrospective Analysis

Background Proximal junctional kyphosis (PJK) is a common postoperative complication after adult spinal deformity (ASD) surgery and may manifest with neurological decline, worsening spinal deformity, and spinal instability, which warrant reoperation. Rates of PJK may be as high as 69.4% after ASD surgery. Objective To evaluate the efficacy of junctional tethers for PJK prophylaxis after multilevel instrumented surgery for ASD with minimum 2-yr follow-up. Methods Single-center retrospective analysis of adult patients (age ≥18 yr) who underwent ASD surgery with index operations performed between November 2010 and June 2016 and achieved minimum 2-yr follow-up. Patients with ASD were subdivided into 3 treatment cohorts based on institutional protocol: no tether (NT), polyethylene tether-only (TO), and tether with crosslink (TC). PJK was defined as a proximal junctional angle (PJA) >10° and 10° greater than the corresponding preoperative measurement. Patient demographics, operative details, standard radiographic scoliosis measurements (including PJA and assessment of PJK), and complications were analyzed. Results Of 184 patients, 146 (79.3%) achieved minimum 2-yr follow-up (mean = 45 mo; mean age = 67 yr; 67.8% women). PJK rates reported for the NT, TO, and TC cohorts were 60.7% (37/61), 35.7% (15/42), and 23.3% (10/43), respectively. PJK rates among TC patients were significantly lower than NT (P = .01601). Conclusion Junctional tethers with crosslink significantly reduced the incidence of PJK and revisions for PJK among ASD patients treated with long-segment posterior instrumented fusions who achieved minimum 2-yr follow-up.
Syntonic Refuge: performing LabSynthE

LabSynthE is a collective of faculty and graduate students at The University of Texas at Dallas in the School of Arts, Technology, and Emerging Communication. The lab is a framework for situated le...
TRANSIT COST ALLOCATION MODEL DEVELOPMENT

The analysis indicates that a one-variable model based on vehicle miles does not account for the route operating efficiencies in terms of operating speed as well as vehicle utilization. A two-variable model consisting of unit cost factors for vehicle miles and vehicle hours more accurately reflects the route efficiency in terms of operatig speed, but does not account for economy relative to vehicle utilization. The three-variable cost allocation model consisting of unit cost factors for vehicle miles, vehicle hours, and peak vehicles more accurately reflects both efficiency measures. The analysis of route costs suggest that the greater the difference in the speed and peak vehicle utilization of a route from the system-wide average, the larger the disparity in individual route cost results from the three formulas. The paper recommends utilization of a three-variable cost allocation model for operational planning purposes.
11 – Energy from Alcohol

This chapter discusses the aspects of ethanol metabolism, relevant to the energy production and effects of ethanol on body weight and composition. Ethanol provides a substantial caloric contribution to the typical western diet. However, its metabolism does not provide the expected amount of energy in physiologically predictable or useful forms. Because the energy-related effects of ethanol are dependent on an individual's health with regard to the presence of absence of alcoholism. The effects of acetate and acetaldehyde, primary metabolites of ethanol metabolism are discussed with regard to energy dis-position. Ethanol serves not only as a substrate for metabolic oxidation, but also regulates endocrine pathways that control: metabolism, substrate mobilization and transport, feeding behavior, and satiety, ethanol's effects on endocrine function. The rate of alcohol consumption provides a considerable amount of energy to the typical western diet, especially in social drinkers where calories derived from ethanol are in addition to that obtained in their food. However, the actual amount of energy derived from the metabolism of ethanol, both thermogenic and metabolic forms, has proven difficult to determine. The basis for these discrepancies may include racial, gender, endocrine, metabolic factors, and alcohol-related toxicity.
Root-shoot interaction in the greening of wheat seedlings grown under red light

Wheat seedlings grown with roots exposed to constant red light (300-500 micromoles m-2 s-1) did not accumulate chlorophyll in the leaves. In contrast, seedlings grown with their roots shielded from light accumulated chlorophylls. Chlorophyll biosynthesis could be induced in red-light-grown chlorophyll-deficient yellow plants by either reducing the red-light intensity at the root surface to 100 micromoles m-1 s-1 or supplementing with 6% blue light. The inhibition of chlorophyll biosynthesis was due to impairment of the Mg-chelatase enzyme working at the origin of the Mg-tetrapyrrole pathway. The root-perceived photomorphogenic inhibition of shoot greening demonstrates root-shoot interaction in the greening process.
The effect of mass loading on the sensitivity of shock accelerometers

The mass loading effect on the sensitivity of shock accelerometers was studied. A method for conversion of a velocity signal to an acceleration signal was presented. The sensitivities of an Endevco 2270 accelerometer for two different mass loads at a shock level of 10,000 m/s 2 were measured. The acceleration signal converted from the velocity output of the vibrometer was compared with the output of the accelerometer affected by mass loading.
Molecular mechanisms of temperature adaptation

Thermal perception is a fundamental physiological process pertaining to the vast majority of organisms. In vertebrates, environmental temperature is detected by the primary afferents of the somatosensory neurons in the skin, which express a 'choir' of ion channels tuned to detect particular temperatures. Nearly two decades of research have revealed a number of receptor ion channels that mediate the perception of several temperature ranges, but most still remain molecularly orphaned. Yet even within this well-researched realm, most of our knowledge largely pertains to two closely related species of rodents, mice and rats. While these are standard biomedical research models, mice and rats provide a limited perspective to elucidate the general principles that drive somatosensory evolution. In recent years, significant advances have been made in understanding the molecular mechanism of temperature adaptation in evolutionarily distant vertebrates and in organisms with acute thermal sensitivity. These studies have revealed the remarkable versatility of the somatosensory system and highlighted adaptations at the molecular level, which often include changes in biophysical properties of ion channels from the transient receptor potential family. Exploiting non-standard animal models has the potential to provide unexpected insights into general principles of thermosensation and thermoregulation, unachievable using the rodent model alone.
Zinc-free and reduced T4 gene 32 protein binds single-stranded DNA weakly and fails to stimulate UvsX-catalyzed homologous pairing.

The functional role of Zn(II) binding by T4 gene 32 protein (gp32), a single-stranded DNA-binding protein, has been investigated by assessing the capacity of a well-characterized metal-free gp32 derivative to function in vitro as an accessory protein of T4 uvsX-catalyzed homologous pairing. Metal-free gp32 was prepared upon reaction of cysteine thiolates with methylmethanethiol-sulfonate to form the mixed disulfide Cys-SSCH3 or S-methylated species. Far and near ultraviolet circular dichroism spectroscopy suggest a moderate but easily detected change in the far UV region, accompanied by only a minor alteration in the near UV region, relative to the Zn(II)-containing protein. Restoration of the wild-type spectral features is accomplished upon the addition of 2 mM dithiothreitol and excess Zn(II) but not dithiothreitol alone. Unlike wild-type gp32, apo S-methylated gp32 shows weak binding to the recombination substrate, single-stranded M13mp19, and fails to stimulate homologous pairing with a linear M13mp19 duplex substrate by uvsX protein. Complete reactivation of the apo S-methylated protein as a recombination-accessory protein is achievable in situ in the presence of reducing agent and sufficient exogenous Zn(II), but not one or the other alone. Analogous results are obtained with S-methylated C166S (Cys166–>Ser) gp32, revealing that only the metal-liganding cysteines participate in the reconstitution. These findings suggest that formation of the Zn(II) chelate is directly linked to single-stranded DNA binding and functional efficacy of gp32 in DNA metabolism.
Alterations of Protein Synthesis in Arbovirus-Infected L Cells

Lust, George (Fort Detrick, Frederick, Md.). Alterations of protein synthesis in arbovirus-infected L cells. J. Bacteriol. 91:1612–1617. 1966.—Cellular protein synthesis and ribonucleic acid (RNA) synthesis in mouse L cells were markedly depressed 1 hr after infection with Venezuelan equine encephalomyelitis virus. Host RNA and protein synthesis were inhibited more rapidly by the virus infection than by actinomycin D. In cells infected 4 hr, a cytoplasmic RNA polymerase was demonstrated which was absent in uninfected cells. At this time, deoxyribonucleic acid-directed RNA synthesis catalyzed by the nuclear RNA polymerase was inhibited in vitro in enzyme preparations from nuclei of virus-infected cells. For optimal activity, the cytoplasmic RNA polymerase required the four nucleoside triphosphates, Mg++, and RNA. The enzyme was insensitive to actinomycin D and deoxyribonuclease, indicating that it catalyzed RNA-directed RNA synthesis. Attempts to purify the induced polymerase further were unsuccessful. Fresh preparations had to be used because the enzymatic activity was unstable.
Aspirin and Pancreatic Cancer Pain

In a controlled double blind study of analgesics for the pain of gastrointestinal cancer, aspirin (650 mg) was significantly more effective than placebo and superior to orally used codeine sulfate (60 mg). The effectiveness of aspirin was not specific for carcinoma of the pancreas, however, because the same pattern of pain relief was found in colonic carcinoma.
Placental expression profile of imprinted genes impacts birth weight

The importance of imprinted genes in regulating feto-placental development has been long established. However, a comprehensive assessment of the role of placental imprinted gene expression on fetal growth has yet to be conducted. In this study, we examined the association between the placental expression of 108 established and putative imprinted genes and birth weight in 677 term pregnancies, oversampled for small for gestational age (SGA) and large for gestational age (LGA) infants. Using adjusted multinomial regression analyses, a 2-fold increase in the expression of 9 imprinted genes was positively associated with LGA status: BLCAP [odds ratio (OR) = 3.78, 95% confidence interval (CI): 1.83, 7.82], DLK1 [OR = 1.63, 95% CI: 1.27, 2.09], H19 [OR = 2.79, 95% CI: 1.77, 4.42], IGF2 [OR = 1.43, 95% CI:1.31, 2.40], MEG3 [OR = 1.42, 95% CI: 1.19, 1.71], MEST [OR = 4.78, 95% CI: 2.64, 8.65], NNAT [OR = 1.40, 95% CI: 1.05, 1.86], NDN [OR = 2.52, 95% CI: 1.72, 3.68], and PLAGL1 [OR = 1.85, 95% CI: 1.40, 2.44]. Fo...
Making Do, Making Home: Borders and the Worlds of Chinatown and Sonoratown in Early Twentieth-Century Los Angeles

The racialized and gendered regulations of national borders through the industrialization of neighborhood geographies had a tremendous impact on daily work, home architecture, and social relationships in the Chinatown and Sonoratown neighborhoods of Los Angeles during the early twentieth century. Using space and gender as analytic lenses, this article examines the built environments of house courts in Sonoratown and Chinatown as windows into the everyday lives of Mexican and Chinese Angelenos. Women’s ordering of domestic space and intimate labor in and around the home spaces—in particular, their use of common spaces like courtyards and alleyways—blurred the categories that city and state agents designated for family, home, and nation, as they shaped conceptions of community that exceeded the logic of the nation-state.
Geomorphic characterization of four shelf-sourced submarine canyons along the U.S. Mid-Atlantic continental margin

Abstract Shelf-sourced submarine canyons are common features of continental margins and are fundamental to deep-sea sedimentary systems. Despite their geomorphic and geologic significance, relatively few passive margin shelf-breaching canyons worldwide have been mapped using modern geophysical methods. Between 2007 and 2012 a series of geophysical surveys was conducted across four major canyons of the US Mid-Atlantic margin: Wilmington, Baltimore, Washington, and Norfolk canyons. More than 5700 km2 of high-resolution multibeam bathymetry and 890 line-km of sub-bottom CHIRP profiles were collected along the outer shelf and uppermost slope (depths of 80-1200 m). The data allowed us to compare and contrast the fine-scale morphology of each canyon system. The canyons have marked differences in the morphology and orientation of canyon heads, steepness and density of sidewall gullies, and the character of the continental shelf surrounding canyon rims. Down-canyon axial profiles for Washington, Baltimore and Wilmington canyons have linear shapes, and each canyon thalweg exhibits morphological evidence for recent, relatively small-scale sediment transport. For example, Washington Canyon displays extremely steep wall gradients and contains ~100 m wide, 5–10 m deep, v-shaped incisions down the canyon axis, suggesting modern or recent sediment transport. In contrast, the convex axial thalweg profile, the absence of thalweg incision, and evidence for sediment infilling at the canyon head, suggest that depositional processes strongly influence Norfolk Canyon during the current sea-level high-stand. The north walls of Wilmington, Washington and Norfolk canyons are steeper than the south walls due to differential erosion, though the underlying cause for this asymmetry is not clear. Furthermore, we speculate that most of the geomorphic features observed within the canyons (e.g., terraces, tributary canyons, gullies, and hanging valleys) were formed during the Pleistocene, and show only subtle modification by Holocene processes active during the present sea-level high-stand.
An asymptotic analysis for directional solidification of a diffusion-dominated binary system

A model is presented that describes the steady-state transport of heat and solute in a diffusion-dominated, directional solidification system characterized by weak melt flows (e.g. under microgravity conditions). The heat transfer between the ampoule and melt can be asymmetric hence the two-dimensional geometry of the model is restricted to a Cartesian coordinate system. The solution procedure involves a coupled asymptotic/numerical approach. The asymptotic expansions are based upon the assumptions that the ampoule aspect ratio, the heat exchange between the ampoule and sample, and the slope of the liquidus line are small. These scalings lead to geometric boundary layer solutions around the solidifying front. The solidifying interfacial shape, thermal, flow, and solutal profiles can be analytically evaluated as functions of the heater temperature profile, heater translation rate, and material properties of the system. In the regime of interest, the flow is decoupled from the temperature and the concentration fields in the geometric boundary layer. Lateral corrections to the shape of the interface are determined by axial and lateral diffusive transport of heat, and diffusive transport of solute. The latter modify the melting point of the interface and the temperature gradients local to the interface. Lateral solute segregation is predicted to increase with solidification rate.
Structural role of amino acids 99-110 in recombinant human erythropoietin.

Erythropoietin is the prime regulator of red blood cell production. Previous studies demonstrated that antipeptide antibodies to amino acids 99-119 and 111-129 bind to two non-overlapping domains and inhibit the hormone's action (Sytkowski, A.J. & Donahue, K. A. (1987) J. Biol. Chem. 262, 1161-1165). Oligonucleotide-directed mutagenesis now shows that amino acids 99-110 (domain 1) but not 119-129 (domain 2) are important to erythropoietin's structure and function. Mutagenesis of wild-type human erythropoietin cDNA was used to produce a series of mutant proteins with sequential deletion of three adjacent amino acids and insertion of the sequence Glu-Phe across the two domains. Transient expression in COS-7 cells revealed 2.0-kb transcripts encoded by all of the cDNAs. Domain 2 mutants exhibited specific biological activities similar to that of the wild type. In contrast, domain 1 mutants were not secreted. In vitro transcription and translation of the domain 1, domain 2 and wild-type cDNAs resulted in the isolation of 23.5-kDa and 32-kDa proteins in the absence or presence of pancreatic microsomes, respectively, consistent with efficient translation of all of the mutants and equivalent post-translational processing of each protein. The data suggest that mutation within domain 1 results in the intracellular biosynthesis of erythropoietins with altered structure, rendering them subject to rapid degradation. The bioassay of erythropoietins synthesized entirely in vitro demonstrated that domain 1 mutants were inactive, whereas both wild type and domain 2 mutant hormones exhibited biologic activity. The results are consistent with a critical role for amino acids 99-110 in the structure of human erythropoietin.