cssi/SciSciGPT-SciSciNet huggingface dataset:
Quantum Computation: a Tutorial

This tutorial is the first part of a series of two articles on quantum computation. In this first paper, we present the field of quantum computation from a broad perspective. We review the mathematical background and informally discuss physical implementations of quantum computers. Finally, we present the main primitives used in quantum algorithms.
Anatomic runoff score predicts cardiovascular outcomes in patients with lower extremity peripheral artery disease undergoing revascularization.

Background Although the presence, extent, and severity of obstruction in patients with lower extremity peripheral artery disease (LE PAD) affect their functional status, quality of life, and treatment, it is not known if these factors are associated with future cardiovascular events. We empirically created an anatomic runoff score (ARS) to approximate the burden of LE PAD and determined its association with clinical outcomes. Methods We evaluated all patients with LE PAD and bilateral angiography undergoing revascularization in a community-based clinical study. Primary clinical outcomes of interest were (1) a composite of all-cause death, myocardial infarction (MI), and stroke and (2) amputation-free survival. Cox proportional hazards models were created to identify predictors of clinical outcomes. Results We evaluated 908 patients undergoing angiography, and a total of 260 (28.0%) patients reached the composite end point (45 MI, 63 stroke, and 152 death) during the study period. Anatomic runoff score ranged from 0 to 15 (mean 4.7; SD 2.5) with higher scores indicating a higher burden of disease, and an optimal cutpoint analysis classified patients into low ARS ( P P P P P Conclusions After adjustment for clinical factors, the LE PAD ARS was an independent predictor of future cardiovascular morbidity and mortality in a broadly representative patient population undergoing revascularization for symptomatic PAD. A clinically useful anatomic scoring system, if validated, may assist clinicians in risk stratification during the course of clinical decision making.
The effect of heat and low pH on selected viral and bacterial fish pathogens

Abstract The thermal and pH resistance of five representative salmonid fish pathogens were tested to examine the efficacy of procedures currently used for processing fish viscera to be incorporated into fish diets. Aeromonas salmonicida, Mycobacterium chelonei, Renibacterium salmoninarum, infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) were suspended in buffered medium at pH 7.0 or 4.0, or in fish silage at pH 4.0 and their survival at selected temperatures was determined. When suspended in medium at pH 7.0, A. salmonicida, M. chelonei and IHNV were sensitive to temperatures in a range of 40–60°C. Renibacterium salmoninarum and IPNV were both more resistant to that temperature range in the neutral pH medium; however, each was inactivated by conditions simulating pasteurization. The combination of heat and low pH currently used for the processing and pasteurization of fish viscera should ensure that the resulting fish food is safe against spreading infectious diseases.
Pd-Mediated cross-coupling of aryl, alkenyl, and alkynyl stannanes with chiral 2-bromo oxazolines

Abstract Chiral 2-bromo-2-oxazolines couple with appropriately substituted organostannanes mediated by Pd-catalysts to furnish 2-substituted oxazolines in good to moderate yields.
The Interactive Roles of Leaders' Emotional Labor In Followers' Outcomes

An increasing stream of research has shown that leaders' emotions have substantial impact on followers' attitudes and performance. However, this line of research has barely explored the role of lea...
One-Trial Learning and Biphasic Time Course of Performance in the Goldfish

Goldfish (N = 408) spontaneously swam against flowing water into a calm-water well. After a single trial punished by brief electric shock, the fish avoided the well, as indexed by increased latencies of reentry. Avoidance declined during the first minute after shock, then rose to a peak 1 hour later. The biphasic time course is compatible with the two-store theory of memory formation.
The effectiveness of cost-effectiveness analysis in containing costs

OBEJCTIVE: Although cost-effectiveness analyses (CEAs) have been advocated as a tool to critically appraise the value of health expenditures, it has been widely hoped that they might also help contain health care costs. To determine how often they discourage additional expenditures, we reviewed the conclusions of recently published CEAs.
Formation of thiolate and phosphonate adlayers on indium-tin oxide: Optical and electronic characterization

Variable angle reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to investigate adlayers on indium−tin oxide (ITO) and fluorine-doped tin oxide surfaces. A close-packed, ordered adlayer was observed to form on both surfaces by 1-hexadecanethiol through thiolate−indium or thiolate−tin bonding on the two surfaces, respectively. Additionally, a close-packed, ordered adlayer of 12-phosphonododecanoic acid was observed to form on ITO through a phosphonate−indium bonding interaction. The chain length dependence of adlayer formation was studied using alkane thiols of varying chain lengths, and the enthalpic factors affecting adlayer formation were modeled using density functional theory calculations along with periodic boundary conditions.
Adhesion between Phosphatidylethanolamine Bilayers

The goal of this study is to provide additional information on the short-range interactions that determine the adhesion energy between bilayer surfaces. The specific problem concerns the hydration properties of bilayers of the membrane lipid phosphatidylethanolamine (PE), which imbibe much less water than bilayers composed of the other common ziwtterionic membrane lipid, phosphatidylcholine (PC). The osmotic stress/X-ray diffraction method was used to measure pressure−distance relations for PE and PC bilayers containing known mole fractions of the charged lipid phosphatidic acid (PA). The addition of 20 mol % PA to either PC or PE bilayers swelled the bilayers by an amount predictable from electrostatic double-layer theory. However, whereas the addition of 5 mol % PA disjoined PC bilayers, it did not change the fluid space between PE bilayers. By calculating the magnitude of the electrostatic pressure necessary to disjoin the bilayers, we estimate the adhesion energy for gel phase PE bilayers to be about ...
In vitro Sensitivity of Human Leukemic Cells to X-Rays.∗:

Summary1. This study determined the in vitro sensitivity to x-rays of the blood cells of 12 patients with chronic lymphocytic leukemia. The methods used were time-lapse cinemicrography and direct observations with phase microscopy. Non-irradiated leukemic lymphocytes survived 4 to 7 days under the in vitro conditions. Dosages of 5 to lOOOr reduced survival time of blood cells of 11 out of 12 patients with chronic lymphocytic leukemia. 2. Blood cells of one patient with chronic lymphocytic leukemia were resistant to dosages of lOOOr but were killed by irradiation with 4000r. This patient was given clinically 180r spray x-ray treatment but the white cell count showed only a slight decline and the patient failed to respond to the therapy. 3. Irradiation with lOOOr produced little or no effect on survival of blood cells from 3 patients with chronic myelogenous leukemia and 3 with acute leukemia.
Abstract A78: Using primary osteosarcoma samples to study tumor heterogeneity

Osteosarcoma (OS) is an aggressive pediatric bone malignancy that affects 400 children per year in the United States. It has one of the lowest 5 yr. survival rates of any pediatric cancer. In order to gain a better understanding of the pathogenesis of OS, we have established a panel of patient derived xenografts (PDX). Through a three institution collaboration, we have obtained 52 OS patient samples, 38 pre-chemotherapy biopsies and 16 post induction chemotherapy resections. These samples were implanted into immunocompromised mice either subrenal or intradermal and allowed to expand. We were able to generate 20 PDX samples (38% take rate) and have characterized 7 of them. This includes one pre and post-chemo pair from the same patient. The PDX samples had similar histology to the original patient biopsy including formation of osteoid, which is a hallmark of OS. Tumor propagating cells (TPCs) are a rare subset of cells with in the tumor that are thought to contain the properties necessary to propagate and give rise to all cells found in the original tumor. TPCs are also thought to be resistant to chemotherapy and thus, it is important to identify and study these cells to gain a better understand the biology that underlies the TPCs ability to propagate the tumor. To identify the TPCs in OS, we selected a panel of cell surface markers that have been associated with TPCs in other cancer types and assessed for their expression across multiple OS PDX samples. CD49f, CD146 and CD90 were expressed heterogeneously in most PDX samples. There was also a high degree of overlap in the expression of these three markers in a subset of OS cells. To determine if the heterogenenous staining observed has a biological significance, we sorted CD49f positive and negative cells and implanted them in serial dilutions into immunocompromized mice. Although no difference in tumor instance was observed, CD49f + tumor cells formed significantly larger tumors than those arising from CD49f- tumor cells. Thus, CD49f expression may mark a more proliferative subset of cells within the bulk tumor. Further studies are currently ongoing to define the functional relevance of CD146 and CD90 expression and their role in tumor propagation and tumor morphology using OS PDX samples. To further characterize these OS PDX samples, we have employed RNA sequencing technology. To date we have obtained RNAseq on 4 pre-chemotherapy and 3 post-chemotherapy patient samples. Analysis of this dataset is ongoing and may provide insight into mechanisms of chemoresistance. Citation Format: Leanne C. Sayles, Justin Vincent-Tompkins, Charles Chan, Irving Weissman, E. Alejandro Sweet-Cordero. Using primary osteosarcoma samples to study tumor heterogeneity. [abstract]. In: Proceedings of the AACR Special Conference on Pediatric Cancer at the Crossroads: Translating Discovery into Improved Outcomes; Nov 3-6, 2013; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2013;74(20 Suppl):Abstract nr A78.
COMPLEX REGGE POLES AND THE SIGN OF THE TWO-POMERANCHON DISCONTINUITY

Abstract The threshold increase of the two-fireball cross section, with one fireball of large mass and one of small is discussed in terms of complex Regge poles and the sign of the two-Pomeranchon discontinuity. It is shown that even though the cut in the complete amplitude is positive, an approximate Regge representation without complex poles, suitable for a limited energy region, may contain a negative cut. A connection is made between the triple Pomeranchon coupling and the amplitude of the cross-section oscillation associated with the complex poles.
We: Commentary on Papers by Trevarthen, Ammaniti & Trentini, and Gallese

In my discussion of papers by Trevarthen, Ammaniti & Trentini, and Gallese, I situate their work within an emerging paradigm of intersubjectivity. This new model finds philosophic grounding in the work of the phenomenologists (Husserl, Merleau-Ponty, Nancy) as it draws from the multiple sources that come together to comprise the new field of cognitive and affective neurosciences. These papers hold the potential to make relational psychoanalysis more relational by providing new foundations for rethinking the biological, developmental and clinical. What emerges is an intersubjective theory based in motor action and perception that serves as the embodied basis for human culture and community, a shared “we” space of basic human affinity. I extend this conception into the analytic setting with a brief introduction to enactive participation in clinical process.
Filtered density function for large eddy simulation of turbulent reacting flows

A methodology termed the “filtered density function” (FDF) is developed and implemented for large eddy simulation (LES) of chemically reacting turbulent flows. In this methodology, the effects of the unresolved scalar fluctuations are taken into account by considering the probability density function (PDF) of subgrid scale (SGS) scalar quantities. A transport equation is derived for the FDF in which the effect of chemical reactions appears in a closed form. The influences of scalar mixing and convection within the subgrid are modeled. The FDF transport equation is solved numerically via a Lagrangian Monte Carlo scheme in which the solutions of the equivalent stochastic differential equations (SDEs) are obtained. These solutions preserve the Ito-Gikhman nature of the SDEs. The consistency of the FDF approach, the convergence of its Monte Carlo solution and the performance of the closures employed in the FDF transport equation are assessed by comparisons with results obtained by direct numerical simulation ...
Neurovascular decompression with selective partial rhizotomy of the trigeminal nerve for tic douloureux

Abstract Fourteen patients with classic tic douloureux were treated with neurovascular decompression of the trigeminal nerve at its zone of entry into the pons. In addition, selective rhizotomy of the portio major was performed in each patient. This combined procedure gave excellent relief of pain with no recurrences in a 23-month postoperative period. Technical observations and postoperative complications in 3 patients are discussed. Routine selective partial rhizotomy of the sensory root of the trigeminal nerve may be a useful adjunct to neurovascular decompression for tic douloureux.
Domain-specific languages for composing signature discovery workflows

Domain-agnostic signature discovery entails study across multiple scientific disciplines. The cross-disciplinary nature and breadth of this work requires that existing executable applications be integrated with new capabilities into workflows, representing a wide range of user tasks. An algorithm may be written in multiple programming languages for various hardware platforms, and so workflow composition requires integrating executables from any number of remote hosts. This raises an engineering issue on how to generate web service wrappers for these heterogeneous executables and to compose them into a scientific workflow environment (e.g., Taverna). In this position paper, we summarize our work on two simple Domain-Specific Languages (DSLs) that automate these processes. Our Service Description Language (SDL) describes key elements of a signature discovery service and automatically generates its implementation code. The Workflow Description Language (WDL) describes the pipeline of services and generates deployable artifacts for the Taverna workflow management system. We demonstrate our approach with a real-world workflow composed of services wrapping remote executables.
Solid-particle motion in two-dimensional peristaltic flows

Some insight into the mechanism of solid-particle transport by peristalsis is sought experimentally through a two-dimensional model study (§ 2). The peristaltic wave is characterized by a single bolus sweeping by the particle, resulting in oscillatory motion of the particle. Because of fluid-particle interaction and the significant curvature in the wall wave, the peristaltic flow is highly nonlinear and time dependent.For a neutrally buoyant particle propelled along the axis of the channel by a single bolus, the net particle displacement can be either positive or negative. The instantaneous force acting upon the particle and the resultant particle trajectory are sensitive to the Reynolds number of the flow (§ 3 and 4). The net forward movement of the particle increases slightly with the particle size but decreases rapidly as the gap width of the bolus increases. The combined dynamic effects of the gap width and Reynolds number on the particle displacement are studied (§ 5). Changes in both the amplitude and the form of the wave have significant effects on particle motion. A decrease in wave amplitude along with an increase in wave speed may lead to a net retrograde particle motion (§ 6). For a non-neutrally buoyant particle, the gravitational effects on particle transport are modelled according to the ratio of the Froude number to the Reynolds number. The interaction of the particle with the wall for this case is also explored (§ 7).When the centre of the particle is off the longitudinal axis, the particle will undergo rotation as well as translation. Lateral migration of the particle is found to occur in the curvilinear flow region of the bolus, leading to a reduction in the net longitudinal transport (§ 8). The interaction of the curvilinear flow field with the particle is further discussed through comparison of flow patterns around a particle with the corresponding cases without a particle (§ 9).
DOS‐tips: Making Do

You may have had the experience of typing a document in which a specific word or phrase, perhaps a company or library name, occurs repeatedly in the text. To save yourself the trouble of frequent retyping, simply enter a code such as “@1” everywhere the repeating word or phrase occurs. Then, when the whole document is entered, use your word processor's “Find and Replace” function to replace “@1” with the actual text. You can have several of these “macros” in one document by using codes “@2”, “@3”, etc. (Watch out if you get to “@10”. A “@1” is hiding in there!)
Platonic Orthonormal Wavelets

Abstract We classify all orthonormal wavelets which occur in the L 2 space of the faces of a platonic solid.
Oxidase activity of a flavin‐dependent thymidylate synthase

Flavin-dependent thymidylate synthases (FDTS) catalyze the production of dTMP from dUMP and N5,N10-methylene-5,6,7,8-tetrahydrofolate (CH2H4folate). In contrast to human and other classical thymidylate synthases, the activity of FDTS depends on a FAD coenzyme, and its catalytic mechanism is very different. Several human pathogens rely on this recently discovered enzyme, making it an attractive target for novel antibiotics. Like many other flavoenzymes, FDTS can function as an oxidase, which catalyzes the reduction of O2 to H2O2, using reduced NADPH or other reducing agents. In this study, we exploit the oxidase activity of FDTS from Thermatoga maritima to probe the binding and release features of the substrates and products during its synthase activity. Results from steady-state and single-turnover experiments suggest a sequential kinetic mechanism of substrate binding during FDTS oxidase activity. CH2H4folate competitively inhibits the oxidase activity, which indicates that CH2H4folate and O2 compete for the same reduced and dUMP-activated enzymatic complex (FDTS–FADH2–NADP+–dUMP). These studies imply that the binding of CH2H4folate precedes NADP+ release during FDTS activity. The inhibition constant of CH2H4folate towards the oxidase activity was determined to be rather small (2 μm), which indicates a tight binding of CH2H4folate to the FDTS–FADH2–NADP+–dUMP complex.
The Heat Budget of a Body of Water of Varying Volume1

When the heat budget is applied to determine the evaporation from a lake of varying volume, the water budget must be considered. Using the continuity of the system a modified equation for evaporation from the heat budget has been developed. The modified equation has a term in the denominator that includes a base temperature used in the calculations. This equation is flexible in use and permits minimizing errors due to unknown quantities in the water budget.
Distributed CSMA with pairwise coding

We consider distributed strategies for joint routing, scheduling, and network coding to maximize throughput in wireless networks. Network coding allows for an increase in network throughput under certain routing conditions. We previously developed a centralized control policy to jointly optimize for routing and scheduling combined with a simple network coding strategy using max-weight scheduling (MWS) [9]. In this work we focus on pairwise network coding and develop a distributed carrier sense multiple access (CSMA) policy that supports all arrival rates allowed by the network subject to the pairwise coding constraint. We extend our scheme to optimize for packet overhearing to increase the number of beneficial coding opportunities. Simulation results show that the CSMA strategy yields the same throughput as the optimal centralized policy of [9], but at the cost of increased delay. Moreover, overhearing provides up to an additional 25% increase in throughput on random topologies.
Oral pigmentation and a method of removing it.

Abstract 1. 1. A simple method for removing certain types of melanin pigmentation from the anterior gingivae has been described. 2. 2. The incidence, histology, and physiology of such pigmentation has been discussed briefly.
The 3'-tail of a forked-duplex sterically determines whether one or two DNA strands pass through the central channel of a replication-fork helicase.

DnaB helicase is a ring-shaped hexamer that unwinds DNA at a replication fork. To understand how this protein interacts with DNA during unwinding, DnaB from Thermus aquaticus was incubated with chemically modified forked-duplex DNA substrates and the unwinding rates were measured. Unwinding was inhibited by modifications made to the 5'-tail, but not the 3'-tail, suggesting that the helicase interacts with the 5'-tail but not the 3'-tail during unwinding. Using oligonucleotides of mixed polarity, it was confirmed that DnaB translocates in the 5' to 3' direction as it unwinds DNA. A substrate was synthesized that contained two duplexes in tandem. Experiments involving various modifications of this tandem duplex demonstrated that when the 3'-tail is short, two stands of DNA pass through the central channel of DnaB with no resultant unwinding. Thus, the role of the 3'-tail in stimulating unwinding has been elucidated. The 3'-tail does not bind to DnaB during unwinding, but sterically determines whether one or two DNA strands pass through the central channel of DnaB. Furthermore, a new substrate for DnaB locomotion has been discovered. DnaB may actively translocate in the 5' to 3' direction along single-stranded DNA, even when a complementary strand is also present within the protein's central channel. This new mode of action may regulate DnaB activity by inhibiting unwinding at regions of DNA that are not forked. Furthermore, this new function for DnaB may coordinate abortion of leading and lagging strand replication if a nick is encountered on the leading strand.
Esophagitis and Hiatal Hernia

Esophagitis manifested by epigastric pain and distress, retrosternal burning, belching, and dysphagia, and, in the more severe cases, esophagogastric ulceration with bleeding and cicatricial changes is oftenest due to esophageal hiatal hernia of the sliding type. Schmidt 3 at the Mayo Clinic found that among 170 patients with benign regurgitant ulceration at the esophagogastric junction 158 had that type of hiatal hernia. A 47-year-old housewife who was first seen with the complaint of epigastric and retrosternal distress of many years duration. Several years previously she had been diagnosed as having "stomach ulcer" and had received some help from an ulcer program. However, the symptoms continued to progress. Intake of food intensified the symptoms and caused belching and regurgitation, much to the annoyance of the patient and her associates. Temporary relief was afforded by the ingestion of a popular antacid dispensed at cigaret counters. When first seen, the patient was using
Monte carlo simulation of macromolecular ionization by nanoelectrospray

Electrospray ionization (ESI) is commonly used in macromolecular mass spectrometry, yet the dynamics of macromolecules in ESI droplets are not well understood. In this study, a Monte Carlo based model was developed, which can predict the efficiency of electrospray ionization for macromolecules, i.e., the number of macromolecular ions produced per macromolecules electrosprayed. The model takes into account ESI droplet evaporation, macromolecular diffusion within the droplet, droplet fissions, and the statistical nature of the ESI process. Two idealized representations of macromolecular analytes were developed, describing cluster prone, droplet surface inactive macromolecules and droplet surface active macromolecules, respectively. It was found that surface active macromolecules are preferentially ionized over surface inactive cluster prone macromolecules when the initial droplet size is large and the analyte concentration in solution is high. Simulations showed that ESI efficiency decreases with increasing initial droplet size and analyte molecular weight, and is influenced by analyte surface activity, the properties of the solvent, and the variance of the droplet size distribution. Model predictions are qualitatively supported by experimental measurements of macromolecular electrospray ionization made previously. Overall, this study demonstrates the potential capabilities of Monte Carlo based ESI models. Future developments in such models will allow for more accurate predictions of macromolecular ESI intensity.
Tracing Genetic Lineages of Captive Desert Tortoises in Arizona

Abstract We genotyped 180 captive desert tortoises (Gopherus agassizii) from Kingman (n = 45), Phoenix (n = 113), and Tucson (n = 22), Arizona, USA, to determine if the genetic lineage of captives is associated with that of wild tortoises in the local area (Sonoran Desert). We tested all samples for 16 short tandem repeats and sequenced 1,109 base pairs of mitochondrial DNA (mtDNA). To determine genetic origin, we performed assignment tests against a reference database of 997 desert tortoise samples collected throughout the Mojave and Sonoran Deserts. We found that >40% of our Arizona captive samples were genetically of Mojave Desert or hybrid origin, with the percentage of individuals exhibiting the Mojave genotype increasing as the sample locations approached the California, USA, border. In Phoenix, 11.5% were Sonoran–Mojave crosses, and 8.8% were hybrids between desert tortoise and Texas tortoise (G. berlandieri). Our findings present many potential implications for wild tortoises in the Sonoran ...
Weight gain prevention in young adults: design of the study of novel approaches to weight gain prevention (SNAP) randomized controlled trial.

Weight gain during young adulthood is common and is associated with increased cardiovascular risk. Preventing this weight gain from occurring may be critical to improving long-term health. Few studies have focused on weight gain prevention, and these studies have had limited success. SNAP (Study of Novel Approaches to Weight Gain Prevention) is an NIH-funded randomized clinical trial examining the efficacy of two novel self-regulation approaches to weight gain prevention in young adults compared to a minimal treatment control. The interventions focus on either small, consistent changes in eating and exercise behaviors, or larger, periodic changes to buffer against expected weight gains. SNAP targets recruitment of six hundred young adults (18–35 years) with a body mass index between 21.0-30.0 kg/m2, who will be randomly assigned with equal probability to: (1) minimal intervention control; (2) self-regulation with Small Changes; or (3) self-regulation with Large Changes. Both interventions receive 8 weekly face-to-face group sessions, followed by 2 monthly sessions, with two 4-week refresher courses in each of subsequent years. Participants are instructed to report weight via web at least monthly thereafter, and receive monthly email feedback. Participants in Small Changes are taught to make small daily changes (~100 calorie changes) in how much or what they eat and to accumulate 2000 additional steps per day. Participants in Large Changes are taught to create a weight loss buffer of 5–10 pounds once per year to protect against anticipated weight gains. Both groups are encouraged to self-weigh daily and taught a self-regulation color zone system that specifies action depending on weight gain prevention success. Individualized treatment contact is offered to participants who report weight gains. Participants are assessed at baseline, 4 months, and then annually. The primary outcome is weight gain over an average of 3 years of follow-up; secondary outcomes include diet and physical activity behaviors, psychosocial measures, and cardiovascular disease risk factors. SNAP is unique in its focus on weight gain prevention in young adulthood. The trial will provide important information about whether either or both of these novel interventions are effective in preventing weight gain. ClinicalTrials.gov, NCT01183689
Genomewide Screen for Negative Regulators of Sirtuin Activity in Saccharomyces cerevisiae Reveals 40 Loci and Links to Metabolism

Sirtuins are conserved proteins implicated in myriad key processes including gene control, aging, cell survival, metabolism, and DNA repair. In Saccharomyces cerevisiae, the sirtuin Silent information regulator 2 (Sir2) promotes silent chromatin formation, suppresses recombination between repeats, and inhibits senescence. We performed a genomewide screen for factors that negatively regulate Sir activity at a reporter gene placed immediately outside a silenced region. After linkage analysis, assessment of Sir dependency, and knockout tag verification, 40 loci were identified, including 20 that have not been previously described to regulate Sir. In addition to chromatin-associated factors known to prevent ectopic silencing (Bdf1, SAS-I complex, Rpd3L complex, Ku), we identified the Rtt109 DNA repair-associated histone H3 lysine 56 acetyltransferase as an anti-silencing factor. Our findings indicate that Rtt109 functions independently of its proposed effectors, the Rtt101 cullin, Mms1, and Mms22, and demonstrate unexpected interplay between H3K56 and H4K16 acetylation. The screen also identified subunits of mediator (Soh1, Srb2, and Srb5) and mRNA metabolism factors (Kem1, Ssd1), thus raising the possibility that weak silencing affects some aspect of mRNA structure. Finally, several factors connected to metabolism were identified. These include the PAS-domain metabolic sensor kinase Psk2, the mitochondrial homocysteine detoxification enzyme Lap3, and the Fe-S cluster protein maturase Isa2. We speculate that PAS kinase may integrate metabolic signals to control sirtuin activity.
Dermatomyositis associated with bronchiolitis obliterans organizing pneumonia (BOOP).

Bronchiolitis obliterans organizing pneumonia (BOOP) is rarely associated with dermatomyositis and may be resistant to conventional corticosteroid therapy under this circumstance. We present a case of BOOP associated with dermatomyositis that responded to a combination of cyclophosphamide and corticosteroid therapy after corticosteroid treatments, alone, had failed. We believe this case shows it is important to recognize that facial rash in the presence of respiratory distress may represent dermatomyositis with BOOP and aggressive treatment may be necessary for resolution of pulmonary symptoms. (J Am Acad Dermatol 1999;40:328-30.)
Evaluation of a brief tailored motivational intervention to prevent early childhood caries.

Objectives This pragmatic randomized trial evaluated the effectiveness of a tailored educational intervention on oral health behaviors and new untreated carious lesions in low-income African-American children in Detroit, Michigan.
SpCoel1: a Sea Urchin Profilin Gene Expressed Specifically in Coelomocytes in Response to Injury.

SpCoel1 is a single copy gene that is specifically expressed in most of the coelomocytes of the adult purple sea urchin, Strongylocentrotus purpuratus. The 4-kb transcript from this gene has a relatively short (426 nucleotide) open reading frame (ORF) with long 3' and 5' untranslated regions. The ORF encodes a protein that has strong amino acid sequence similarity to profilins from yeast to mammals. Transcript titrations of SpCoel1 show significant increases per coelomocyte in animals that have been physiologically challenged. Increases in transcript levels are of similar magnitudes between animals receiving different treatments, such as injuries from needle punctures or from injections of foreign cells. The evidence presented here implies a molecular mechanism by which this lower deuterostome defense system responds to external insult, viz that an external "injury signal" activates a signal transduction system, which in turn mediates the alterations in cytoskeletal state that are required for coelomocyte activation.
Effects of ungulate herbivory on aspen, cottonwood, and willow development under forest fuels treatment regimes

Herbivory by domestic and wild ungulates can dramatically affect vegetation structure, composition and dynamics in nearly every terrestrial ecosystem of the world. These effects are of particular concern in forests of western North America, where intensive herbivory by native and domestic ungulates has the potential to substantially reduce or eliminate deciduous, highly palatable species of aspen (Populus tremuloides), cottonwood (Populus trichocarpa), and willow (Salix spp.). In turn, differential herbivory pressure may favor greater establishment of unpalatable conifers that serve as ladder fuels for stand-replacing fires. The resulting high fuel loads often require silvicultural fuels reductions to mitigate fire risk, which in turn may facilitate additional recruitment of deciduous species but also additional herbivory pressure. Potential interactions of ungulate herbivory with episodic disturbances of silviculture, fire, and other land uses are not well documented, but are thought to operate synergistically to affect forest dynamics. We evaluated individual and joint effects of ungulate herbivory and fuels reduction treatments in grand fir (Abies grandis) and Douglas-fir (Psuedotsuga menziezii) forests that dominate large areas of interior western North America. We applied fuels reduction treatments of mechanical thinning and prescribed fire and then evaluated the responses of aspen, cottonwood, and willow species to these treatments (N = 3) versus areas of no treatment (N = 3), and to exclusion from ungulate herbivory versus areas subjected to extant herbivory by free-ranging cattle (Bos taurus), elk (Cervus elaphus), and mule deer (Odocoileus hemionus). Densities of deciduous species were >4 times higher in response to fuels reduction treatments (84.4 individuals/ha) compared to areas of no treatment (19.7 individuals/ha). Additionally, when ungulates were excluded from fuels treated sites, the density of cottonwood was >5 times higher (122.5 individuals/ha) than fuels treated sites subjected to extant herbivory (24.3 individuals/ha). Similarly, densities of Populus spp. and Salix spp. were >3 times higher (211.6 individuals/ha) on fuels treated sites excluded from ungulate herbivory versus fuels treated sites subjected to extant herbivory (66.1 stems/ha). Deciduous species subjected to extant ungulate herbivory also were significantly lower in height, canopy surface area, and canopy volume than the same species inside the ungulate exclosures. Recruitment and long-term survival of aspen, cottonwood, and willow species in coniferous forests of interior western North America require a combination of episodic disturbances such as silviculture and fire to facilitate deciduous plant recruitment, followed by reductions in grazing pressure by domestic and wild ungulates during the time intervals between episodic disturbances to facilitate plant establishment, growth and survival.
Aggression, Sociability, and Roommate Friendship: New Findings Translated Into a Resource for Self‐Determined Choices

Many individuals with intellectual disabilities (ID) live with others, but little is known about roommate compatibility, and there are no resources available to assist in the selection process. People with ID and those who support them need practical information in order to make more informed and therefore better choices. Self-determination also should play an increased role in this complicated choice. This paper provides a practical, data-based resource that can be incorporated into a self-determined process of roommate selection. We conducted two studies, uncovered new information, and translated the findings into something that individuals and their supporters can use. The first study examined data from a community sample. In the second investigation, we replicated and expanded key findings in an institutional setting. Taken together, the findings of these studies are generalizable to a larger portion of the population. The results indicated that for the trait of sociability, similarity was the key to friendship. On the other hand, for aggressiveness, it was not similarity, but the total aggression in the pair that was (inversely) related to friendship. A practical multistep resource is provided for adults with ID and their advocates to help with the selection of a roommate who might also become a friend.
Vector finite elements for electromagnetic field computation

A novel structure for the finite-element analysis of vector fields is presented. This structure uses the affine transformation to represent vectors and vector operations over triangular domains. Two-dimensional high-order vector elements are derived that are consistent with Whitney forms. One-form elements preserve the continuity of the tangential components of a vector field across element boundaries, while two-form elements preserve the continuity of the normal components. The one-form elements are supplemented with additional variables to achieve pth order completeness in the range space of the curl operator. The resulting elements are called tangential vector finite elements and provide consistent, reliable, and accurate methods for solving electromagnetic field problems. >
Connective Tissue Mast Cells Exhibit Time-Dependent Degranulation Heterogeneity

Previous studies have identified two ultrastructurally distinct forms of mast cell (MC) degranulation following activation. Immunoglobulin E (IgE)-mediated reactions are characterized by a very rapid swelling and fusion of MC granules and abrupt mediator release. In certain chronic disease states (e.g., bullous pemphigoid), there is "piecemeal" degranulation with a more-gradual mediator release effected by microvesicular transport of "pieces" of granules to the cell surface. It is unclear whether these two degranulation patterns are determined by the different natures of the stimuli, heterogeneity among responding MC granules, or temporal factors. To investigate these issues, we have carried out electron microscopic studies with skin biopsies obtained from ragweed-sensitive subjects 15 and 30 s and 1, 3, 5, and 10 min after intradermal ragweed injection. "Anaphylactic"-type granule changes began by 15 s after ragweed injection and were complete by 5 min; unaffected granules were juxtaposed with granules that were swollen and fused. The remaining granules subsequently underwent changes in appearance similar to those seen in piecemeal degranulation. However, microvesicular transport of granule components to the surface was not observed. These findings indicate that skin MC changes in sites of IgE-mediated reactions include not only the typical very rapid anaphylactic degranulation but also a slower onset of gradual alteration of other granules, frequently within the same MC. These different patterns could reflect MC granule heterogeneity with attendant different responses to IgE-mediated stimuli.
VCAM-1 Activates Phosphatidylinositol 3-Kinase and Induces p120Cbl Phosphorylation in Human Airway Smooth Muscle Cells

VCAM-1 is a member of the Ig superfamily of receptors the expression of which is up-regulated on human airway smooth muscle (ASM) cells following stimulation with inflammatory mediators. The function of these receptors in adhesion is well known, but there is growing recognition that they also possess "outside-in" signaling functions, such as cytoskeletal reorganization, calcium mobilization, and cytokine release. The present study examined the activation of extracellular signal-regulated kinase and phosphatidylinositol 3-kinase (PI3K) in ASM cells following VCAM-1 engagement. VCAM-1 ligation activated extracellular signal-regulated kinase 2 and resulted in increased expression of cyclin D1, yet there was neither p27(kip1) degradation nor an increase in smooth muscle cell DNA synthesis. VCAM-1 ligation, however, augmented the proliferative response to submitogenic concentrations of epidermal growth factor. VCAM-1 engagement also stimulated a rapid increase in PI3K activity. This was associated with phosphorylation of the adapter protein p120(Cbl) and an increase in Cbl-associated PI3K activity. These studies suggest that VCAM-1 is linked to multiple signaling pathways in human ASM cells and may function to augment growth factor-induced responses.
Frame-Based Immobilization and Targeting for Stereotactic Body Radiation Therapy

Abstract Frame-based stereotactic body radiation therapy (SBRT), such as that conducted with Elekta’s Stereotactic Body Frame, can provide an extra measure of precision in the delivery of radiation to extracranial targets, and facilitates secure patient immobilization. In this paper, we review the steps involved in optimal use of an extra-cranial immobilization device for SBRT treatments. Our approach to using frame-based SBRT consists of 4 steps: patient immobilization, tumor and organ motion control, treatment/planning correlation, and daily targeting with pretreatment quality assurance. Patient immobilization was achieved with the Vac-Loc bag, which uses styrofoam beads to conform to the patient’s shape comfortably within the body frame. Organ and motion control was assessed under fluoroscopy and controlled via a frame-mounted abdominal pressure plate. The compression screw was tightened until the diaphragmatic excursion range was
A novel polymer of tubulin forms the conoid of Toxoplasma gondii.

Toxoplasma gondii is an obligatory intracellular parasite, an important human pathogen, and a convenient laboratory model for many other human and veterinary pathogens in the phylum Apicomplexa, such as Plasmodium, Eimeria, and Cryptosporidia. 22 subpellicular microtubules form a scaffold that defines the cell shape of T. gondii. Its cytoskeleton also includes an intricate apical structure consisting of the conoid, two intraconoid microtubules, and two polar rings. The conoid is a 380-nm diameter motile organelle, consisting of fibers wound into a spiral like a compressed spring. FRAP analysis of transgenic T. gondii expressing YFP-α-tubulin reveals that the conoid fibers are assembled by rapid incorporation of tubulin subunits during early, but not late, stages of cell division. Electron microscopic analysis shows that in the mature conoid, tubulin is arranged into a novel polymer form that is quite different from typical microtubules.
Response of White Leghorn Chickens of Various Genetic Lines to Infection with Avian Leukosis Virus Subgroup J

SUMMARY. In Experiment 1, chickens from various white leghorn experimental lines were inoculated with strain ADOL-Hcl of subgroup J avian leukosis virus (ALV-J) either as embryos or at 1 day of age. At various ages, chickens were tested for ALV-J induced viremia, antibody, and packed cell volume (PCV). Also, at 4 and 10 wk of age, bursal tissues were examined for avian leukosis virus (ALV)-induced preneoplastic lesions with the methyl green–pyronine (MGP) stain. In Experiment 2, chickens harboring or lacking endogenous virus 21 (EV21) were inoculated with strain ADOL-Hcl of ALV-J at hatch. All embryo-inoculated chickens in Experiment 1 tested positive for ALV-J and lacked antibody throughout the experimental period of 30 wk and were considered viremic tolerant, regardless of line of chickens. By 10 wk of age, the incidence of ALV-J viremia in chickens inoculated with virus at hatch varied from 0 (line 0 chickens) to 97% (line 15I5); no influence of ALV-J infection was noted on PCV. Results from microscopic examination of MGP-stained bursal tissues indicate that ALV-J can induce typical ALV-induced transformation in bursal follicles of white leghorn chickens. Lymphoid leukosis and hemangiomas were the most common ALV-J–induced tumors noted in chickens in Experiment 1. At termination of Experiment 2 (31 wk of age), 54% of chickens harboring EV21 were viremic tolerant compared with 5% of chickens lacking EV21 after inoculation with ALV-J at hatch. The data indicate that genetic differences among lines of white leghorn chickens, including the presence or absence of EV21, can influence response of chickens to infection with ALV-J.
Nursing and Home Care Perspective of Painful Diabetic Neuropathy

The obesity epidemic has resulted in an increase incidence of Type 2 Diabetes Mellitus (T2DM) in all age groups. Diabetic peripheral neuropathy (DPN), a well-known microvascular complication of diabetes increases with age and is most prevalent in older adults with longstanding diabetes [1]. In one survey of 255 diabetic patients, the reported average age, number of years with diabetes, and years with painful DPN (PDPN) was 61, 12, and 6.4 years, respectively [2]. Further, as diabetes rises in all age groups worldwide, with an estimated 4.4 % prevalence by 2030, the potential for complications such as DPN with and without pain is likely to increase [3]. Considering the global epidemic of T2DM across the life span and the increase in predicted life expectancy, the need for better access to health care and in particular nursing care for the prevention and management of diabetes becomes evident.
The natural history of penetrating ulcers of the iliac arteries

Objective The natural history of penetrating ulcers of the iliac arteries (PUIA) has not been previously described. The potential for degeneration into pseudoanerysm and rupture are feared complications. It is hypothesized that PUIA, similar to their thoracic aortic counterparts, signal impending vascular catastrophe. Methods A search of computed tomography (CT) angiography reports for the words, "penetrating ulcer" was performed. Patients with PUIA who underwent CT imaging from October 2010 to August 2011 were identified. Their clinical course was followed through December 2014. If patients with PUIA had additional vascular pathology that necessitated intervention, it was performed. A prospective and retrospective review of the imaging was performed when possible. Associated iliac diameter and ulcer dimensions were measured for patients with repeat imaging (n = 22). Demographic characteristics were compared for patients who were identified as having penetrating ulcers of the abdominal aorta. Mann-Whitney U , Fisher exact, and Pearson correlation coefficient tests were performed for statistical analysis. Results The calculated incidence of PUIA for patients who underwent CT imaging was 0.3%. The age at the time of diagnosis was 70.7 ± 10.0 years and the cohort included 28 male patients (82.3%). Median clinical and imaging follow-up was 42.0 (range, 1-82) months and 40.5 (range, 1-77) months. Most patients had a history of hypertension (82.4%), hyperlipidemia (76.5%), and tobacco use (70.6%). Twenty-one patients (61.8%) had concomitant aneurysms not necessarily associated with the PUIA. Although no PUIA rupture occurred, the population was sick because seven patients (20.6%) were deceased at the study end. Only one individual presented with symptoms that could possibly be attributed to their PUIA. Repeat imaging was performed for 22 patients (64.7%). The calculated median iliac artery diameter growth rate through the PUIA was 0.1 (range, 0-4.1) mm/y. Conclusions PUIA are generally slow-growing and are found incidentally. Most patients with PUIA were in their eighth decade with a history of hypertension and tobacco use. Patients with PUIA frequently have concurrent aortic aneurysm disease that requires intervention. The mortality for this population was high, but was not caused by rupture of a PUIA. Diameter changes noted in the PUIA during follow-up did not suggest ulcer treatment would improve survival.