Photosystem I Measurements in Mutants B4 and F8 of Chlamydomonas

In the report “Oxygenic photoautotrophic growth without photosystem I” by J. W. Lee et al. ([19 July 1996, p. 364][1]) ([1][2]) and elsewhere ([2][3]), it is said that mutants of the green alga Chlamydomonas that lacked detectable levels of functional Photosystem I (PSI) were capable of photoreduction of atmospheric carbon dioxide, autotrophic growth, and sustained simultaneous photoevolution of H2 and O2. The absence of PSI in the mutant strains B4 and F8 used in our work ([1][2], [2][3]) was confirmed by physical, biochemical, and genetic techniques. Subsequent analyses in our own laboratories, however, as well as those of colleagues to whom we have sent the mutants, indicate that there is variability in the PSI content of the cultures that depends on growth conditions. While some strains retain undetectable amounts of P700, others contain variable (0 to 20%) amounts of wild-type P700. We stand by our original measurements, which showed a lack of detectable P700 in strains F8 and B4 at the time of these studies ([1][2], [2][3]). 1. [↵][4]1. J. W. Lee, 2. C. V. Tevault, 3. T. G. Owens, 4. E. Greenbaum , Science 273, 364 (1996); [OpenUrl][5][Abstract][6] ; see also 1. J. M. Olson , ibid. 275, 996 (1997); [OpenUrl][7] ; T. G. Owens, J. W. Lee, C. V. Tevault, E. Greenbaum, ibid. , p. 996. 2. [↵][8]1. E. Greenbaum, 2. J. W. Lee, 3. C. V. Tevault, 4. S. L. Blankinship, 5. L. J. Mets , Nature 376, 438 (1995). [OpenUrl][9][CrossRef][10][Web of Science][11] [1]: /lookup/doi/10.1126/science.273.5273.364 [2]: #ref-1 [3]: #ref-2 [4]: #xref-ref-1-1 "View reference 1 in text" [5]: {openurl}?query=rft.jtitle%253DScience%26rft.stitle%253DScience%26rft.issn%253D0036-8075%26rft.aulast%253DLee%26rft.auinit1%253DJ.%2BW.%26rft.volume%253D273%26rft.issue%253D5273%26rft.spage%253D364%26rft.epage%253D367%26rft.atitle%253DOxygenic%2BPhotoautotrophic%2BGrowth%2BWithout%2BPhotosystem%2BI%26rft_id%253Dinfo%253Adoi%252F10.1126%252Fscience.273.5273.364%26rft_id%253Dinfo%253Apmid%252F8662525%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [6]: /lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6Mzoic2NpIjtzOjU6InJlc2lkIjtzOjEyOiIyNzMvNTI3My8zNjQiO3M6NDoiYXRvbSI7czoyNDoiL3NjaS8yNzcvNTMyMy8xNjUuMy5hdG9tIjt9czo4OiJmcmFnbWVudCI7czowOiIiO30= [7]: {openurl}?query=rft.jtitle%253Dibid.%26rft.volume%253D275%26rft.spage%253D996%26rft.atitle%253DIBID%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [8]: #xref-ref-2-1 "View reference 2 in text" [9]: {openurl}?query=rft.jtitle%253DNature%26rft.volume%253D376%26rft.spage%253D438%26rft.atitle%253DNATURE%26rft_id%253Dinfo%253Adoi%252F10.1038%252F376438a0%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [10]: /lookup/external-ref?access_num=10.1038/376438a0&link_type=DOI [11]: /lookup/external-ref?access_num=A1995RM63900056&link_type=ISI