cssi/SciSciGPT-SciSciNet huggingface dataset:
Efficient Autoproteolytic Processing of the MHV-A59 3C-like Proteinase from the Flanking Hydrophobic Domains Requires Membranes

The replicase gene of the coronavirus MHV-A59 encodes a serine-like proteinase similar to the 3C proteinases of picornaviruses. This proteinase domain is flanked on both sides by hydrophobic, potentially membrane-spanning, regions. Cell-free expression of a plasmid encoding only the 3C-like proteinase (3CLpro) resulted in the synthesis of a 29-kDa protein that was specifically recognized by an antibody directed against the carboxy-terminal region of the proteinase. A protein of identical mobility was detected in MHV-A59-infected cell lysates. In vitro expression of a plasmid encoding the 3CLpro and portions of the two flanking hydrophobic regions resulted in inefficient processing of the 29-kDa protein. However, the efficiency of this processing event was enhanced by the addition of canine pancreatic microsomes to the translation reaction, or removal of one of the flanking hydrophobic domains. Proteolysis was inhibited in the presence of N-ethylmaleimide (NEM) or by mutagenesis of the catalytic cysteine residue of the proteinase, indicating that the 3CLpro is responsible for its autoproteolytic cleavage from the flanking domains. Microsomal membranes were unable to enhance the trans processing of a precursor containing the inactive proteinase domain and both hydrophobic regions by a recombinant 3CLpro expressed from Escherichia coli. Membrane association assays demonstrated that the 29-kDa 3CLpro was present in the soluble fraction of the reticulocyte lysates, while polypeptides containing the hydrophobic domains associated with the membrane pelletes. With the help of a viral epitope tag, we identified a 22-kDa membrane-associated polypeptide as the proteolytic product containing the amino-terminal hydrophobic domain.
EFFECTS OF AUXINS ON DEVELOPMENT OF APRICOT SEEDS AND SEEDLINGS

APPLYING THE research results of Crane (1953, 1955) to commercial apricot culture, many California orchardists in the past few years have sprayed trees with 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) to reduce drop, hasten maturity and increase fruit size. Because of the possibility that 2A4,5-T sprayed on trees during seed development might affect the seeds and subsequent seedlings, seeds from Tilton trees that had been sprayed with 2,4,5-T (100 mg./l.) in 1954 were routinely planted in the nursery after a month of stratification. The very few seedlings produced (4.6 per cent as contrasted with 53.4 per cent from controls) were extremely retarded and abnormal. The results reported belowv deal with more refined experiments on the effects of auxin treatment of parent-trees on seed development, germination and seedling growth. They should serve as a warning to nurserymen against the use of such seeds for propagation purposes. Of greater significance here is that they add to the accumulation of evidence that auxin treatments may produce delayed morphogenic and/or physiological effects without causing any morphological aberrancies shortly after auxin application.
Rate‐Adaptive Cardiac Pacing in Children Using a Minute Ventilation Biosensor

YABEK, S.M., ET AL.: Rate-Adaptive Cardiac Pacing in Children Using a Minute Ventilation Biosensor.Chronotropic integrity is required for a normal cardiac output response to exercise. We evaluated a rate-adaptive ventricular demand pacemaker (Telectronics, META-MV) which uses minute ventilation as the sensed physiological variable for adjusting pacing rate, in seven young patients with a mean age of 11.4 years. All patients had clinically significant bradycardia related to complete heart block (n = 4) or sinus node dysfunction (n = 3). For the entire group, paced heart rates increased from 70 ± 10 beats/min to 151 ± 19 beats/min with exercise testing. The onset of rate adaptation took < 30 seconds. Changes in paced rate were linearly related to workload, VO2 (5.9 to 20.7 mL/min/kg) and minute ventilation (8–65 L/min). The decline in pacing rate after exercise was related directly to the gradual decrease in minute ventilation and VO2. Our data show that minute ventilation closely and accurately reflects the metabolic demands of varying workloads in children and can be used to achieve physiological, rate-adaptive pacing.
Characterization of insulin-like growth factor-binding proteins in the uterus and conceptus during early conceptus elongation in cattle

As a first step in determining the role that insulin-like growth factor (IGF)-binding proteins (BPs) may have in regulating initial stages of conceptus elongation in cattle, the type and relative abundance of IGFBPs in serum, uterine tissues, and uterine fluid from pregnant and noninseminated cows on Days 13 and 15 postestrus and in Day 15 conceptuses was evaluated. Uterine and serum samples contained IGFBPs 2, 3, 4, and 5 as determined by immunoprecipitation followed by Western ligand blots of precipitates. Compared with those in uterine and serum samples, IGFBPs in conceptuses and conceptus-conditioned culture media were only faintly detectable. The percentage of the total IGF-I binding activity attributed to IGFBP-3 was greater (p 50%) than in inter- (40%) and intracaruncular (37%) endometrium. Percentage of total binding attributed to IGFBP-2 was greater (p < 0.05) in endometrium and serum ( approximately 30%) than in myometrium (16%) and uterine fluid (9%). Binding activity of certain IGFBPs varied due to day of the estrous cycle or due to pregnancy status. Concentrations of IGF-I in serum were greater (p < 0.05) in nonpregnant (52 +/- 2 ng/ml) than in pregnant (40 +/- 4 ng/ml) cows. Concentration of IGF-I in uterine fluid did not differ due to pregnancy status or stage of cycle (4.4 pg IGF-I/ microg uterine protein). Northern blots revealed mRNAs for IGFBPs 1, 2, 3, 4, and 5 in uterine tissues but not in conceptuses. In situ hybridization indicated that IGFBP-1 mRNA was primarily localized in luminal epithelium of endometrium; IGFBP-2 mRNA was in luminal epithelium and dense stromal cells adjacent to endometrial epithelium; and IGFBP-3 mRNA was in vascular endothelial cells and was more prevalent in myometrium than in endometrium. Tissue specificity and changes in abundance of IGFBPs in the uterus during early conceptus elongation indicate the potential importance of IGFBP regulation of uterine IGFs during this time period.
Multiple observer siting and path planning on a compressed terrain

We examine a smugglers and border guards scenario. We place observers on a terrain so as to optimize their visible coverage area. Then we compute a path that a smuggler would take so as to avoid detection, while also minimizing the path length. We also examine how our results are affected by using a lossy representation of the terrain instead. We propose three new application-specific error metrics for evaluating terrain compression. Our target terrain applications are the optimal placement of observers on a landscape and the navigation through the terrain by smugglers. Instead of using standard metrics such as average or maximum elevation error, we seek to optimize our compression on the specific real-world application of smugglers and border guards.
Development of a computerized dairy herd health data base for epidemiologic research

A computerized information system for disease surveillance, management and herd health service is presented. The objectives are to describe the incentive method of data collection used to maintain the participation of the farmers and veterinarians in the program, to describe techniques used to select cow records for epidemiologic research and to present incidence rates for the major dairy diseases seen during the study period, 1980–1984. Participating farmers recorded animal events on a daily basis, and their veterinarians recorded the results of all diagnoses and treatments. The data were processed and returned to the farmers and the veterinarians in weekly and monthly reports. The farmers and the veterinarians found that the reports returned to them were useful in managing the health of the herd, and this served as the incentive for continued participation in the program. Lactational incidence rates of the most common clinical dairy diseases were 17.5, 15.1 and 12.8% for metritis, mastitis and cystic follicles, respectively.
Correlation of estimated renal function parameters versus 24-hour creatinine clearance in ambulatory elderly.

This study evaluates the correlation between estimated renal function parameters (ie, creatinine clearance by the Cockcroft-Gault and Lott-Hayton formulas, serum creatinine and blood urea nitrogen) and 24-hour creatinine clearance in 15 young and 15 elderly subjects. Correlation coefficients (r) for the elderly group comparing Cockcroft-Gault and Lott-Hayton against 24-hour creatinine clearance were 0.73 (P less than .005) and 0.60 (P less than .02) respectively, and r for the young subjects were 0.37 (P greater than .05) and 0.57 (P less than .05), respectively. In 13 elderly subjects with creatinine clearance of 60 mL/minute or less, four (31%) had blood urea nitrogen of 20 mg/dL or less and 13 (100%) had serum creatinine between 1.0-1.5 mg/dL. We conclude that the formulas of Cockcroft-Gault and Lott-Hayton are valid in ambulatory elderly patients who are functionally independent without severe underlying disease, have normal body weight and are not on medication affecting renal function. Furthermore, in this select elderly population, an apparently normal serum creatinine (1.0-1.5 mg/dL) and blood urea nitrogen (20 mg/dL or less) may frequently represent a 24-hour creatinine clearance of 60 mL/minute or less.
Sexual Dimorphism, the Aging Kidney, and Involvement of Nitric Oxide Deficiency

Females develop less age-dependent loss of renal function, in part because of cardiorenal protective effects of estrogens. The low androgen level in women also may be protective, although the animal and clinical data are controversial. Both estrogen and androgens act through multiple mechanisms, sometimes beneficial, sometimes damaging, which makes it difficult to predict the impact of hormone replacement therapy in an aging population. Nitric oxide (NO) deficiency contributes to age-dependent cardiovascular risk and kidney damage in animal models. The increased oxidative stress of aging impacts at multiple sites in the NO biosynthetic pathway to decrease NO production/action. Endothelial dysfunction develops with aging and is delayed in women, in association with a delayed increase in asymmetric dimethylarginine. Animal data suggest that the aging kidney develops NO deficiency because of changes in the neuronal NO synthase. Relative preservation of NO production in females contributes to the better cardiovascular and renal responses to aging.
The Role of Luminal Nutrients in Intestinal Injury from Mesenteric Reperfusion and Platelet-Activating Factor in the Developing Rat

Necrotizing enterocolitis (NEC) develops primarily after the onset of enteral feeds in the premature infant. The purpose of this study was to evaluate the influence of intestinal luminal nutrients on histologic injury and the oxidant response in a rat model of NEC. On postnatal Days 10 and 35, Sprague-Dawley rats (total n = 81) underwent abdominal laparotomy. A control group received sham-injury only. The ischemia groups received a single intraluminal injection of 0.25 ml (Day 10) or 1.0 ml (Day 35) of lactose (8.6 g/dl), casein (2.2 g/dl), corn oil (4.4 g/dl), or infant formula (Similac ; 20 g/dl). After injection of the nutrient solutions, ischemia groups underwent mesenteric occlusion for 1 hr and intraluminal injection of platelet-activating factor (50 μg/kg). Necropsies were performed after 6 hr or at demise. Intestinal samples were taken for histology, total glutathione (GSH ; an antioxidant), and conjugated dienes (a lipid peroxidation product). Histologic injury was scored from 0 (normal) to 5 (transmural necrosis). Microscopic injury scores in the oil group were significantly higher than the casein group (P < 0.05) and trended toward being higher in the formula group (P = 0.085) at age 10 days. Total GSH activity was significantly higher in the sham groups than all ischemia groups on Day 10 (P < 0.001) and than the corn oil group on Day 35 (P < 0.05). GSH activity did not differ among ischemia groups. Conjugated diene concentrations were significantly higher in the casein group than the lactose and sham groups at age 10 days (P < 0.05) only. We conclude that intraluminal lipids may augment intestinal ischemic injury in the newborn (age 10 days) but not the weanling rat. While oxygenfree radicals were present during injury, lipid peroxidation from oxygen radicals was not responsible for this increase in histologic injury.
Comparison of gibberellin-like substances from cotyledons and phloem exudate collected from cotyledons of Pharbitis nil in relation to photoperiodic flowering

Gibberellin (GA)-like substances were analyzed in extracts from cotyledons and phloem exudate collected from cotyledons in photoinduced and vegetative seedlings of the short-day plant Pharbitis nil Chois. var. Violet, using high performance liquid chromatography (HPLC) and the dwarf rice bioassay, to see whether any specific GA-like substances were transported from the photoinduced cotyledons via phloem. Cotyledon extracts exhibited five peaks of free GA-like activity in HPLC, whereas only one or two active peaks were detected in phloem exudate extracts. The level of free GA-like activity was considerably lower in phloem exudate than in the cotyledons. In five out of six analyses of cotyledons and phloem exudate, there were substantially higher levels of free GA-like substances in photoinduced plants. Conjugated GA-like substances were present in much higher levels than free GA-like substances in the cotyledon extracts but the levels were not influenced by daylength. In phloem exudate extracts there was no conjugated GA-like substances. The free GA-like substances that are transported via phloem cochromatographed with GA5/20 and GA19 on HPLC. These were significantly higher in photoinduced plants and thus could have some influence on the photoperiodically-induced flowering in P. nil.
Role of psychological stress in cortisol recovery from exhaustive exercise among elite athletes

Life-event stress (LES) was used to classify elite athletes (n = 39) into high- and low-LES groups. A repeated measures analysis of variance revealed higher cortisol concentration after a graded exercise lest among the high-LES group relative to the low-LES group, which was maintained for up to 20 hr. Subsequent prospective analyses further indicated that high-LES athletes were more likely lo he symptomatic than low-LES athletes and that elevated cortisol level was positively correlated with symptomatology. To the extent that cortisol is a marker of exercise recovery in competitive athletes. our results suggest that chronic stress prolongs the recovery process, which may potentially widen a window of susceptibility for illness and injury among competitive athletes.
SU-FF-J-88: A Novel Workflow for SBRT Image Guidance Using 4D CT Method

Purpose: We presented a novel clinical workflow to determine interfractional image guidance shifts for SBRT treatment of lung using 4D CT scans, and validated this workflow in phantom studies. Method and Materials: An ITV was first generated on the planning 4D CTimage set and later used for treatment planning. The ITV and the 4D CTimage sets on both the planning and treatment days were loaded into GE AW software. A manual registration was then performed. Using the measurement tool on AW, image guidance shifts were measured based on the same phase of the two CTimage sets. Phantom studies were performed to simulate the entire SBRT process. The target was programmed to be shift for three scenarios: a) L/R and A/P shift; b) Longitudinal shift; and c) combination of L/R, A/P and longitudinal shift. All these phantom studies were repeated for two breathing traces: a sinusoidal and a real patient breathing trace. Results: The phantom studies showed that the differences were within a CT voxel size for all three shifting scenarios between the programmed and the measured shifts using the 4D CT method, for both the sinusoidal and patient breathing traces. We also performed two other validation studies retrospectively for one patient. An average was calculated for the tumor centroid position on 10 phases using a segmentation program. The difference between the simulation day and the treatment day gave the image guidance shifts. The second method used the average intensity projection image on the AW software. The image guidance shifts determined by these two retrospective methods agreed within 2mm with our clinically‐used method. Conclusion: The workflow to determine image guidance shifts for SBRT treatment was validated by phantom studies, and it was further verified retrospectively by two independent methods for one patient with a lungtumor.
Reinforcement-based intensive outpatient treatment for inner city opiate abusers: a short-term evaluation

We evaluated 3-month outcomes for reinforcement-based intensive outpatient treatment (RBT), a new relapse prevention behavior therapy for inner city opiate abusers. The therapy provides abstinence-contingent partial support of housing, food and recreational activities, abstinence-contingent access to social skills and job finding group therapy and non-contingent individual counseling, all in the context of a day treatment program. Heroin abusers (n = 52), contacted at a 3-day detoxification unit, were randomly assigned to RBT (n = 28) or referred to community treatment resources (n = 24) after a staff escort from the detoxification unit. For RBT patients, treatment began on the day of discharge; 61% received partial rent support in a recovery house based on the need for drug-free housing; the remainder were eligible for partial support of utility payments where they lived. Abstinence-based contingencies were in effect for 1 month with three times per week counseling available for an additional 2 months. One month after detoxification, 61% of RBT versus 17% of referral patients were enrolled in outpatient treatment (P < 0.01); RBT patients were significantly less likely than controls to have returned to any drug use; and 50% of RBT versus 21% of controls reported 30 days of abstinence from heroin and cocaine with confirmatory negative urine (P < 0.05). RBT patients had significantly lower scores on the Beck Depression Inventory at 1 month (M = 9.0 versus 17.6 for controls; P < 0.05) and showed evidence of less alcohol use and higher rates of employment. These results establish the short-term efficacy for RBT and support continued development and evaluation of this new outpatient behavioral treatment.
Treatment With Acamprosate in Patients With Schizophrenia Spectrum Disorders and Comorbid Alcohol Dependence

Objective: Alcohol use disorders and schizophrenia frequently co-occur with rates higher than in the general population. There is no consensus on the best treatment for patients with these comorbid conditions. Several clinical trials have shown that acamprosate is superior to placebo in reducing drinking and is particularly effective in sustaining abstinence. No study to date has examined the efficacy of acamprosate in patients with alcohol dependence and comorbid schizophrenia. The aims of this study are to assess the efficacy of acamprosate when compared to placebo in reducing drinking and to examine its effects on schizophrenic symptoms. Methods: This was a double-blind, randomized, 12-week treatment trial of acamprosate versus placebo. Twenty-three recently abstinent patients with diagnosed alcohol dependence and comorbid schizophrenia, schizoaffective disorder, or psychosis not otherwise specified were included in this study. Results: All participants significantly decreased their drinking during med...
Stress relaxation in fibers: Effect of water and temperature

The effect of water on stress relaxation was studied for several fibers as a function of water temperature and other parameters. A quantitative measure of the effect was developed. A statistical model was developed, and it was found that the observed behavior could be interpreted in terms of a redistribution of forces among the various bonds within the fiber. The measure of the water effect, as defined here, could be used to study the connection between stress relaxation and other fiber properties.
Use of noncontact mapping and saline-cooled ablation catheter for sinus node modification in medically refractory inappropriate sinus tachycardia.

Background Inappropriate sinus tachycardia (IST) is characterized by heart rate (HR) increase out of proportion to stress level. Radiofrequency (RF) modification of the sinus node (SN) is an accepted treatment modality for medically refractory IST. We describe a new technique using noncontact mapping and a saline irrigated catheter for SN modification. Methods Seven consecutive patients with medically refractory IST were referred for ablation. Intrinsic heart rate (IHR) was calculated with complete autonomic blockade by atropine and propranolol. Isoproterenol (ISO) 1 mcg/min was initiated and increased to 10 mcg/min. Site of earliest activation was tagged at each dose of ISO once stable HR was achieved. RF ablation to target site of earliest activation at peak HR on ISO 10 mcg/min was performed. With any change in P-wave morphology, activation was reassessed and the new site of earliest activation targeted. Endpoint was a decrease in HR and change in P-wave morphology in lead III and aVF. Results Five of seven patients had abnormal IHR. Mean number of RF lesions was 25 (10-52). All patients had either flattening of the P wave or development of negative P waves in leads III and aVF post RF associated with a decrease in HR of > or = 25% from baseline off ISO. A caudal shift of the site of early activation compared with baseline was observed. One patient who had a prior SN modification developed symptomatic intermittent junctional bradycardia and required an atrial pacemaker 2 weeks later. The other 6 patients in follow-up from 6 to 24 months had no further IST. Conclusions Noncontact mapping using the described technique in conjunction with the saline-cooled ablation catheter for SN modification in the treatment of IST may provide effective HR control.
Enhancing the synthetic efficacy of thorium tetrachloride bis(1,2-dimethoxyethane) with added 1,2-dimethoxyethane: Preparation of metallocene thorium dichlorides

Abstract The synthetic efficacy of thorium tetrachloride bis(dimethoxyethane), ThCl4(DME)2, is improved by adding 1,2-dimethoxyethane (DME) to the reaction mixture. The syntheses of the known metallocene dichlorides (C5Me5)2ThCl2 (1), (1,2,4-tBu3-C5H2)2ThCl2 (2), and (C5Me4Et)2ThCl2 (3) are used to demonstrate this finding. The full characterization and X-ray crystal structure of (C5Me4Et)2ThCl2 (3) is also reported.
The stability of thermally stratified plane Poiseuille flow

In studying the stability of a thermally stratified fluid in the presence of a viscous shear flow, we have a situation in which there is an important interaction between the mechanism of instability due to the stratification and the Tollmien-Schlichting mechanism due to the shear. A complete analysis has been carried out for the Benard problem in the presence of a plane Poiseuille flow and it is shown that, although Squire's transformation can be used to reduce the three-dimensional problem to an equivalent two-dimensional one, a theorem of Squire's type does not follow unless the Richardson number exceeds a certain small negative value. This conclusion follows from the fact that, when the stratification is unstable and the Prandtl number is unity, the equivalent two-dimensional problem becomes identical mathematically to the stability problem for spiral flow between rotating cylinders and, from the known results for the spiral flow problem, Squire's transformation can then be used to obtain the complete three-dimensional stability boundary. For the case of stable stratification, however, Squire's theorem is valid and the instability is of the usual Tollmien—Schlichting type. Additional calculations have been made for this case which show that the flow is completely stabilized when the Richardson number exceeds a certain positive value.
Spatial variation in the response of tree rings to normal faulting during the Hebgen Lake Earthquake, Southwestern Montana, USA

Abstract Tree rings have frequently been used to identify the effects of earthquakes on forests, but little is known about spatial variation in the response of trees to intraplate normal faulting. This paper documents and describes the effects of tree location (distance from and position above or below the fault scarp), size and age on the response of tree rings to the 1959 magnitude 7.5 Hebgen Lake earthquake, which occurred along a normal fault in the Gallatin National Forest in southwestern Montana. Core samples from 88 trees were collected along nine 100-m transects straddling the Hebgen scarp, and from 28 additional large-diameter trees near the scarp. The most common tree-ring response to the earthquake was a suppression in growth, usually lasting for several years. Among samples from the transects, suppressions were significantly more common below vs. above the scarp, but this pattern was not found among the large tree samples. Distance of trees (within 58 m) from the fault scarp had little effect on tree-ring responses. These results illustrate the importance of interactions between tree location and tree size/age in identifying tree-ring responses to earthquakes. Smaller, younger trees required the direct movement of the downthrown block below the scarp to incur sufficient damage to record a suppression, whereas larger, older trees were damaged even on the stationary slope above the scarp. The small effect of distance from the scarp on suppressions suggests that event-response trees may be found further from a fault than previously thought.
Bovine pancreatic DNase I binds very tightly to DNA fragments and may be mistaken for putative endogenous nuclear proteins convalently bound to DNA

Using published methods for the isolation of nuclear proteins tightly bound to DNA, and resistant to removal by SDS or 16-BAC detergent and urea, several new protein bands in the region of 55 kd and 62 kd on SDS gel and 43 kd and 70 kd on 16--BAC gel electrophoresis were identified in extracts of avian erythroid nuclei. These bands were radiolabelled by subjecting the DNA--protein complexes to nick--translation in the presence of [32P]--dCTP, followed by prolonged digestion with excess bovine DNase I. Amino acid sequence analysis shows that these bands contain DNase I. These results indicate that DNase I can form stable complexes with DNA, and suggest that DNase I--DNA complexes may be mistakenly identified as nuclear proteins covalently bound to DNA.
Research Note: Effect of Carbonated Drinking Water on Production Performance and Bone Characteristics of Laying Hens Exposed to High Environmental Temperatures

An experiment was conducted to determine the effects of providing heat-stressed laying hens with carbonated drinking water on production performance and tibia bone breaking strength. A total of 32 commercial laying hens (89 wk of age) were housed in an environmental chamber and exposed to 1 wk of a constant thermoneutral temperature (21 C), followed by 6 wk of a daily heat stress temperature cycle (34 C for 8 h; 21 C for 16 h). One half of the hens received tap water and the other half were provided carbonated water immediately after housing. Following 6 wk of heat stress temperature exposure, all hens were killed by cervical dislocation and left tibia bone strength measurements were taken. Hen-day egg production, egg weight, egg yield, feed consumption, and feed efficiency did not differ between water treatments; however, egg specific gravity was depressed for hens provided carbonated versus tap drinking water. Left tibia bone breaking strength per 100 g body weight was improved (P < or = .05) for hens provided with carbonated drinking water during exposure to heat stress temperatures. These results suggest that carbonated drinking water may enhance bone integrity by increasing tibia bone breaking strength of older laying hens exposed to a short-term heat stress period.
Metabolic depression in animals exposed to air after living in a he-O2 environment.

Metabolic depression in animals exposed to air after living in helium-oxygen environment, suggesting denitrogenation period effect
β1-tubulin gene mutation platelets are not macrothrombocytes

To the editor: The brief report by Kunishima et al[1][1] describing a mutation of the β1 tubulin gene affecting microtubule assembly is of interest, but data presented do not support association of the genetic defect with macrothrombocytopenia. The authors stated both the male propositus and his
Quarkonia production in pp, p(d)A and AA Collisions

Abstract Quarkonia ( J / ψ , ψ ′ , ϒ ) production provides a sensitive probe of gluon distributions and their modification in nuclei; and is a leading probe of the hotdense (deconfined) matter created in high-energy collisions of heavy ions. I will discuss our current understanding of the modification of gluon distributions in nuclei and other cold-nuclear-matter effects in the context of recent p-p and p(d)-A quarkonia measurements. Then I will review the latest results for nucleus-nucleus collisions from RHIC, and together with the baseline results from d-A and p-p collisions, discuss several alternative explanations for the observed suppressions and future prospects for distinguishing these different pictures.
Food Hypersensitivity and Otolaryngologic Conditions in Young Children

Objectives/HypothesisTo explore the relationship between food hypersensitivity and common upper aerodigestive disorders found in children younger than 2 years.Study DesignCase control study.SettingTertiary pediatric gastroenterology/otolaryngology clinics.MethodsTwo-year retrospective chart review of a common cohort of children younger than 2 years with suspected cow’s milk protein allergy (CMPA). Patients were managed in both a tertiary pediatric gastroenterology and otolaryngology clinic and compared with a control study group.ResultsOne hundred ninety-one children with CMPA were reviewed. One hundred forty-one had aerodigestive complaints, with 101 having sufficient follow-up to participate in the study. Ninety-one percent of this final patient group demonstrated improved symptoms following dairy elimination. Twenty-five patients (27%) had associated otolaryngologic (ear, nose, and throat [ENT]) issues that had been refractory to maximal gastroesophageal reflux therapy. The most common associated upper...
Abstract LB-345: Functional genomics reveals acute dependencies on polyadenylation for tumor cell mitotic progression

We are using genome-wide synthetic lethal paclitaxel chemosensitizer screens to understand the protein networks that support mitosis in lung tumor cells. Using this approach, we have identified several components of the polyadenylation machinery that are required for a high-fidelity mitosis in the presence of a synthetic lethal dose of paclitaxel. Here, we show depletion of multiple polyadenylation proteins in combination with low-dose paclitaxel causes a G2/M cell cycle block. We show depletion of one of these polyadenylation proteins, symplekin, has profound effects on mitotic spindle assembly resulting in over-duplicated centrosomes and impaired microtubule growth. Furthermore, we use a panel of lung lines to show symplekin is uniquely required for tumor cell mitosis as compared to normal cell mitosis. Our mechanistic studies suggest symplekin may mediate these effects on tumor cell mitosis by interacting with the centrosomally-associated protein transforming acidic coiled coil 3 protein (TACC3). Interestingly, TACC3 is typically expressed during development but becomes aberrantly upregulated in tumor cells. We propose TACC3 may become upregulated during tumorigenesis and engage symplekin to support mitosis in tumor cells. These results suggest polyadenylation components may represent an attractive therapeutic target in combination with already available anti-mitotic agents. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr LB-345.
Work in progress — A multidisciplinary introduction to microfabrication

Microfabrication is a critical area to many branches of science and engineering. In this work-in-progress paper, we describe our plan to introduce microfabrication technology in a comprehensive, cross-curricular way through lectures, demonstrations and experiments from freshman through junior classes across four disciplines (Electrical and Mechanical Engineering, Chemistry, and Physics). Immediately following, in the senior year, will be an opportunity for students in these disciplines to take a multidisciplinary microfabrication capstone course that will serve as a complete introduction to clean room theory and practice. This approach will fundamentally show microfabrication as being based on many disciplines and vital to most modern technologies. Assessment will be done for each level of the project, and assessment results will be used to continuously improve the course over time and gauge the success of attracting students into this area.
Comparison of androgenic effects on food intake and body weight in adult rats

Abstract In order to investigate the interrelationships between sex hormones, food intake, and body weight, three androgens (testosterone propionate (TP), dihydrotestosterone (DHT), and androstenedione (ANDR)) were administered to separate groups of gonadectomized adult rats of both sexes. In males TP increased body weight to the greatest degree, while the effects of DHT, a non-aromatizable androgen, and ANDR were approximately equal to each other. The relationship between each androgen and food intake partly paralleled that of body weight. In females, however, DHT exerted a stronger effect on body weight than TP, and ANDR produced no significant increases: food intake was stimulated by both TP and DHT. As others have indicated, aromatization of testosterone to estrogen then is not critical to the effect of androgens on either food intake or body weight. Furthermore, the fact that increases in body weight are not always accompanied by concomitant changes in food intake suggests that gonadal hormones may exert their effects on body weight primarily through various metabolic processes, not simply through changes in food intake.
Real World Experience with the Wearable External Defibrillator: A False Sense of Security?

(5016 6 10314 PVCs). A PVC burden of $5% was found in 21% of patients with LVSD. The presence of any PVC on an individual ECG was a strong marker of high PVC burden (odds ratio, 34.0 [5.3e219.3], p ! .01). The sensitivity and NPV of any PVC on a 12 lead ECG for high PVC burden $5% was 80% and 94%, respectively (Kappa 5 0.65, P ! 0.001). The number of PVCs on an ECG were also correlated with PVC burden (non-parametric r 5 0.668, P ! 0.001) and patients with 1e2 PVCs and particularly those with O 2 PVCs had significantly (P ! 0.001) elevated PVC burden during Holter (Table). Conclusions: In this study about 20% of patients with LVSD could potentially benefit from a correction of a PVC induced cardiomyopathy. Evidence of any PVC on a 12 lead ECG provided a simple screening tool to identify this patient population in a daily clinical practice.
Investigating new risk reduction and mitigation in the oil and gas industry

Abstract The complexity of the processes and the nature of volatile petroleum products urged the oil and gas industry to utilize various risk assessment techniques to identify potential failure modes that can interrupt operation processes. Consequently, government agencies and nonprofit professional societies guide the industry with regulatory guidelines, standards, and best recommended practices to oversee the operations management, assure safe working environment, and contain failures within tolerable limits. Yet, accidents due to electro-mechanical failures still occur and result in various consequences. Accordingly, critics have raised concerns about the petroleum industry's safety and risk mitigation credentials and question its ability to prevent future major accidents. Therefore, new risk assessment tools need to be introduced to provide decision makers and novice engineers with a diverse perception of potential risks. The aim of this paper is verify the application of Risk in Early Design (RED), a product risk assessment tool, in identifying potential failures in the oil and gas industry. Approximately thirty major accident underwent the RED analysis to verify the software's application to identify and rank potential failure modes.
Vacuum erection device in spinal cord injured men: Patient and partner satisfaction

Abstract Objective: To assess the efficacy of and patient and partner satisfaction with a vacuum erection device (VED) to treat erectile dysfunction of spinal cord injury. Design: Case series. Setting: University hospital outpatient clinic. Patients: Twenty spinal cord injured men with erectile dysfunction and their heterosexual partners, recruited from outpatient population and by advertisement. Intervention: Use of a VED to obtain erections for sexual activity. Main Outcome Measures: Efficacy in obtaining adequate penile erection, and patient and partner satisfaction with the device (survey). Results: At 3 months, 93% of the men and 83% of the women reported rigidity sufficient for vaginal penetration, with an average duration of 18 minutes. These numbers decreased somewhat at the 6-month control. At 6 months, 41% of the men and 45% of the women were satisfied with the device, with premature loss of rigidity during intercourse the most commonly reported complaint. Sixty percent of men and 42% of women indicated an improvement of the sexual relationship. Minor side effects, such as petechiae and penile skin edema, occurred frequently, but there were no complications that required treatment. Conclusion: The VED is effective in many couples in the treatment of erectile dysfunction associated with spinal cord injury. The devices were not universally accepted, but had a significant impact on sexual activity and sexual satisfaction for nearly half the couples. Vacuum erection devices should be presented to SCI men along with other options for treatment of erectile dysfunction.
Metabolism and thermoregulation in desert and montane grasshoppers.

Temperature regulation and oxygen consumption were examined in two species of grasshoppers: Melanoplus sanguinipes from cold alpine tundra at elevation 3,800 m, and Trimerotropis pallidipennis from hot desert habitats at elevation 250 m. Both species utilized behavioral thermoregulation to keep body temperature (T b ) more constant than environmental temperatures (T e ) during the day. The difference in average T b in the two species was much less than the difference in T e 's. Microclimate measurements indicate that temperature regulation is not difficult for M. sanguinipes, but T. pallidipennis must restrict activity for much of the day to avoid heat stress and can easily overheat if it moves into sunlit areas. Oxygen consumption ( $$\dot V{\text{O}}_{\text{2}} $$ ) at average T b and total daily energy expenditures are higher in M. sanguinipes than in T. pallidipennis, as is the Q10 for $$\dot V{\text{O}}_{\text{2}} $$ . These differences may be related to different strategies for energy utilization and predator avoidance.
Hemopoietic Stem Cells in Human Peripheral Blood

A population of lymphocytes, separable from the great majority by virtue of their larger size and their failure to exhibit the rosetting characteristics of thymus-dependent lymphocytes and bursa-equivalent cells, possess true pluripotentiality. On culture in vivo they proliferate and differentiate into erythrocytic, granulocytic, and megakaryocytic progeny. This may be the first clear demonstration of the primitive progenitor blood cell in man.
Induction of AIDS in rhesus monkeys by molecularly cloned simian immunodeficiency virus

Better understanding of the pathogenesis of acquired immunodeficiency syndrome (AIDS) would be greatly facilitated by a relevant animal model that uses molecularly cloned virus of defined sequence to induce the disease. Such a system would also be of great value for AIDS vaccine research. An infectious molecular clone of simian immunodeficiency virus (SIV) was identified that induces AIDS in common rhesus monkeys in a time frame suitable for laboratory investigation. These results provide another strong link in the chain of evidence for the viral etiology of AIDS. More importantly, they define a system for molecular dissection of the determinants of AIDS pathogenesis.
Long-Term Memory Deficits are Associated with Elevated Synaptic ERK1/2 Activation and Reversed by mGluR5 Antagonism in an Animal Model of Autism

A significant proportion of patients with autism exhibit some degree of intellectual disability. The BTBR T(+) Itpr3(tf)/J mouse strain exhibits behaviors that align with the major diagnostic criteria of autism. To further evaluate the BTBR strain's cognitive impairments, we quantified hippocampus-dependent object location memory (OLM) and found that one-third of the BTBR mice exhibited robust memory, whereas the remainder did not. Fluorescence deconvolution tomography was used to test whether synaptic levels of activated extracellular signal-regulated kinase 1/2 (ERK1/2), a protein that contributes importantly to plasticity, correlate with OLM scores in individual mice. In hippocampal field CA1, the BTBRs had fewer post-synaptic densities associated with high levels of phosphorylated (p-) ERK1/2 as compared with C57BL/6 mice. Although counts of p-ERK1/2 immunoreactive synapses did not correlate with OLM performance, the intensity of synaptic p-ERK1/2 immunolabeling was negatively correlated with OLM scores across BTBRs. Metabotropic glutamate receptor (mGluR) 5 signaling activates ERK1/2. Therefore, we tested whether treatment with the mGluR5 antagonist MPEP normalizes synaptic and learning measures in BTBR mice: MPEP facilitated OLM and decreased synaptic p-ERK1/2 immunolabeling intensity without affecting numbers of p-ERK1/2+ synapses. In contrast, semi-chronic ampakine treatment, which facilitates memory in other models of cognitive impairment, had no effect on OLM in BTBRs. These results suggest that intellectual disabilities associated with different neurodevelopmental disorders on the autism spectrum require distinct therapeutic strategies based on underlying synaptic pathology.
Dual of the sum of a linear and linear fractional program

Abstract This paper presents a dual of a mathematical programming problem whose objective function, the sum of linear and linear fractional types, is subjected to linear constraints. The duality theorems for linear programming and those for the linear fractional programming are particular cases for this more general problem.
Microautoradiographic study of the intracellular and extracellular distribution of glycerol-1,3-C14 labeled lipids in experimental connective tissue

out on tissue aliquots removed from the same incubation flasks which contained the tissues used for the chemical analysis of glycer01-1,3-C~~ incorporation by granuloma slices 7-450 days of Using the technic of quantitative microautoradiography, it has been possible to identify incorporation of this isotope and study its localization within the several cell types present in polyvinyl sponge and open wound granulomas. In addition, the presence of glycer01-1,3-C~~ in extracellular location has been demonstrated. Using standard histologic methods designed to preserve or extract lipids from the tissue, it has been found that 75 per cent of the incorporated isotope is labile to such procedures. These findings suggest that lipids (lipoproteins) occur as constituents of connective tissue "ground substance." METHODS
Effective minority-carrier mobility in heavily doped silicon defined by trapping and energy-gap narrowing

A simple model for effective minority-carrier mobility in heavily doped silicon, lower than the extended-state (∼ majority-carrier) mobility, is developed based on the assumption that some of the carriers are trapped in band-tail (or shallow bound) states. The model demonstrates for the first time a correlation between measured mobility and energy-gap narrowing, which is also influenced by band tails. Numerical simulations of temperature-dependent hole transport in the emitters of n+-p-n transistors, which accurately predict the measured (base) currents when the effective hole mobility model and the energy-gap narrowing it implies are used, are discussed.
The results of in situ prosthetic replacement for infected aortic grafts

BACKGROUND: Treatment of aortic graft infection with graft excision and axillofemoral bypass may carry an increased risk of limb loss, aortic stump blowout, and pelvic ischemia. A review of patients with aortic graft infection treated with in situ prosthetic graft replacement was undertaken to determine if mortality, limb loss, and reinfection rates were improved with this technique. METHODS: The clinical data of 25 patients, 19 males and 6 females, with a mean age of 68 years (range 35 to 83), with aortic graft infection, treated between January 1, 1989, and December 31, 1998, by in situ prosthetic graft replacement were reviewed. Follow-up was complete in the 23 surviving patients and averaged 36 months (range 4 to 103). RESULTS: Twenty aortofemoral, 3 aortoiliac, and 2 straight aortic graft infections were treated with excision and in situ replacement with standard polyester grafts in 16 patients (64%), or with rifampin-soaked collagen or gelatin-impregnated polyester grafts in 9 patients (36%). Fifteen patients (60%) had aortic graft enteric fistulas, 8 patients (32%) had abscesses or draining sinuses, and 2 patients (8%) had bacterial biofilm infections. Thirty-day mortality was 8% (2 of 25). There were no early graft occlusions or amputations. There was one late graft occlusion. There were no late amputations. The reinfection rate was 22% (5 grafts). All reinfections occurred in patients operated upon for occlusive disease. Only one reinfection occurred in the rifampin-soaked graft group (11% versus 29%, P = NS). Reinfection tended to be lower in patients with aortoenteric fistulas and without abscess. Autogenous tissue coverage provided statistically significant protection against reinfection. There were no late deaths related to in situ graft infection. CONCLUSIONS: Patients treated with in situ graft replacement had an 8% mortality and 100% limb salvage rate. Reinfection rates were similar to those of extra-anatomic bypass, but a trend of lower reinfection rates with rifampin-impregnated grafts was apparent. Patients with aortoenteric fistula and without abscess appear to be well treated by the technique of in situ prosthetic grafting and autogenous tissue coverage.
Regulation of cadherin expression in nervous system development.

This review addresses our current understanding of the regulatory mechanisms for classical cadherin expression during development of the vertebrate nervous system. The complexity of the spatial and temporal expression patterns is linked to morphogenic and functional roles in the developing nervous system. While the regulatory networks controlling cadherin expression are not well understood, it is likely that the multiple signaling pathways active in the development of particular domains also regulate the specific cadherins expressed at that time and location. With the growing understanding of the broader roles of cadherins in cell–cell adhesion and non-adhesion processes, it is important to understand both the upstream regulation of cadherin expression and the downstream effects of specific cadherins within their cellular context.