Anatomy of the rat medial geniculate body: II. Dendritic morphology.

The medial geniculate body (MGB) of the rat was studied with Golgi methods to determine the distribution of neurons identified by dendritic morphology. These findings were compared with major divisions and constituent nuclei established by somatic and fiber architectonics, and by connections with temporal neocortex (Clerici et al.: Society of Neuroscience Abstracts 12:1272, 1986; 13:325, 1987; Anatomical Record 218:23, 1987; Winer and Larue: Journal of Comparative Neurology 257:282–315, 1987; Clerici and Coleman: Journal of Comparative Neurology 297:14–31, 1990). It was found that an elaboration of the prototypical scheme proposed by Morest (Journal of Anatomy 98:611–630, 1964) for partitioning the mammalian MGB is valid for characterizing the rat MGB. Two predominant categories of principal neuron dendritic patterning were identified: a bushy cell having tufted dendritic fields and a stellate cell with a radiate dendritic domain. Tufted neurons have large caliber dendritic trunks that divide profusely into daughter branches close to the soma with intertwining higher order branches that maintain a relatively restricted dendritic field. Stellate neurons typically emit primary dendrites in all directions that then divide dichotomously at wide angles at subsequent orders of branching to produce a somewhat spheroidal dendritic field. In the present study, the rat MGB is found to be a tripartite structure composed of ventral (MGv), dorsal (MGd), and medial (MGm) divisions, each uniquely characterized by constituent dendritic morphology. The paramount neuronal class of the MGv is the tufted principal cell. In the ventral and ovoid nuclei of the MGv the neuronal orientation of highly oriented bitufted cells is in register with afferent brachial axons. In the ventral nucleus, this arrangement approximates vertical with a dorsomedial tilt most prominent rostrally; in the ovoid nucleus, tufted cells adhere to the double spiraled course of afferent axons. The transition zone between ventral and ovoid nuclei contains tufted neurons that align with radially oriented fibers issuing from the junction of the ovoid and midgeniculate bundles. Bitufted neurons of the marginal zone parallel fibers at the lateral margin of the geniculate. Within the MGd the dorsal and caudodorsal nuclei are characterized by stellate cells with extensive dendritic arbors and bushy neurons with dendritic branches less tufted than those observed in the MGv. The deep dorsal nucleus contains bitufted neurons that polarize with the long axis of the midgeniculate bundle and intermingle with stellate neurons. The suprageniculate nucleus includes neurons with large somata and long, sparsely branched and dorsoventrally oriented dendrites orthagonal to corticothalamic axons, as well as smaller neurons and classical stellate cells. Neurons of the limitans nucleus have elongated dendritic fields directed along the medial border of the auditory thalamus. Among the variety of cell types of the MGm are ones with the largest somata in the MGB, the prominent “magnocellular” neurons. These cells emit only a few gradually tapering dendrites, which are encrusted with appendages; dendrites branch at irregular intervals and lie oblique to incoming axons from the brachium of the inferior colliculus. The MGm also contains smaller neurons, both tufted and radiate in typology, along with more classical stellate cells. The distribution of cells characterized by morphological features of dendrites corresponds to anatomical regions of the MGB identified by cytoarchitecture, myeloarchitecture, and necortical connectivity. The structural of the rat MGB are mostly typical of mammalian forms, which suggests the rat provides a use for the auditory forebrain.