cssi/SciSciGPT-SciSciNet huggingface dataset:
The pressure injury predictive model: A framework for hospital-acquired pressure injuries.

Background Despite decades of research, pressure injuries continue to be a source of significant pain and delayed recovery for patients and substantial quality and cost issues for hospitals. Consideration of the current thinking around pressure injury risk must be evaluated to improve risk assessments and subsequent nursing interventions aimed at reducing hospital-acquired pressure injuries. Design This is a discursive paper using Walker and Avant's (2005) theory synthesis framework to examine the relevance of existing pressure injury models as they align with the current literature. Methods PubMed and CINAHL indexes were searched, first for conceptual models and then for pressure injury research conducted on hospitalised patients for the years 2006-2016. A synthesis of the searches culminated into a new pressure injury risk model. Conclusions Gaps in previous models include lack of attention to the environment, contributing episode-of-care factors and the dynamic nature of injury risk for patients. Through theory synthesis, the need for a new model representing the full risk for pressure injury was identified. The Pressure Injury Predictive Model is a representation of the complex and dynamic nature of pressure injury risk that builds on previous models and addresses new patient, contextual and episode-of-care process influences. The Pressure Injury Predictive Model (PIPM) provides a more accurate picture of the complexity of contextual and process factors associated with pressure injury development. Relevance to clinical practice Using the PIPM to determine risk can result in improved risk identification. This information can be used to implement targeted, evidence-based pressure injury prevention interventions specific to the patient risk profile, thus limiting unwarranted and unnecessary care.
High-fidelity light-shift gate for clock-state qubits

To date, the highest-fidelity quantum logic gates between two qubits have been achieved using the geometric-phase gate in trapped ions, with the two leading variants being the M\o{}lmer-S\o{}rensen gate and the light-shift (LS) gate. Both of these approaches have their respective advantages and challenges. For example, the latter is technically simpler and is natively insensitive to optical phases, but it has not been made to work directly on a clock-state qubit. We present a technique for implementing the LS gate that combines the best features of these two approaches: By using a small ($\ensuremath{\sim}\mathrm{MHz}$) detuning from a narrow (dipole-forbidden) optical transition, we are able to operate an LS gate directly on hyperfine clock states, achieving gate fidelities of $99.74(4)%$ using modest laser power at visible wavelengths. Current gate infidelities appear to be dominated by technical noise, and theoretical modeling suggests a path toward gate fidelity above $99.99%$.
Pharmacokinetic Analysis of Epithelial/Endothelial Cell Barriers in Microfluidic Bilayer Devices with an Air-Liquid Interface

As the range of applications of organs-on-chips is broadening, the evaluation of aerosol-based therapies using a lung-on-a-chip model has become an attractive approach. Inhalation therapies are not only minimally invasive but also provide optimal pharmacokinetic conditions for drug absorption. As drug development evolves, it is likely that better screening through use of organs-on-chips can significantly save time and cost. In this work, bio-aerosols of various compounds including insulin were generated using a jet nebulizer. The aerosol flows were driven through microfluidic bilayer devices establishing an air–liquid interface to mimic the blood–air barrier in human small airways. The aerosol flow in the microfluidic devices has been characterized and adjusted to closely match physiological values. The permeability of several compounds, including paracellular and transcellular biomarkers, across epithelial/endothelial cell barriers was measured. Concentration–time plots were established in microfluidic devices with and without cells; the curves were then utilized to extract standard pharmacokinetic parameters such as the area under the curve, maximum concentration, and time to maximum concentration. The cell barrier significantly affected the measured pharmacokinetic parameters, as compound absorption through the barrier decreases with its increasing molecular size. Aerosolizing insulin can lead to the formation of fibrils, prior to its entry to the microfluidic device, with a substantially larger apparent molecular size effectively blocking its paracellular transport. The results demonstrate the advantage of using lung-on-a-chip for drug discovery with applications such as development of novel inhaled therapies.
Ductoscopy combined X-ray mammography for breast cancer screening

Objective Understand shunde Uistrict Foshan City,Guangdong Province in the incidence of breast cancer in women.Methods The joint selectivity ductoscopy examination of the clinical breast examination screening,breast X-ray radiography examination from March 2011 to January 2013,Shunde District,FoShan City,Guangdong Provice,3 600 bladder than 40 years old woman with breast cancer screening.Results Three thousand six hundred women were found in 10 cases of breast cancer,the detection rate of 278/100 000 (10/3 600).Breast clinical examination found 1 313 cases of breast abnormalities,142 cases of breast lumps,nipple discharge line fiberoptic ductoscopy to cheek the 100 cases,X-ray radiography examination of four and more than 72 cases,accounted for a total of 45.19％ of the screening population (1 627/3 600).Conclusions Joint ductoscopy X-ray radiography examination of breast cancer screening in the normal population can help to detect early breast cancer,and to provide the basis for early clinical treatment. Key words: Breast neoplasms; Carcinoma; Ductoscopy; X-ray mammography; Screening; Epidemiology
Applied Bachelier Option Pricing: Where Both Assets Are Price Uncertain, and Option Asset Value

This paper builds on Louis Bachelier model for option pricing work by the author. The focus is to generalise the option model for where both assets are price uncertain, adapting the bivariate normal to the model; the paper also works on defining the underlying asset values based on this model, in doing so proposes the option definition be revised to recognise as a contract to ‘complete’ a purchase or sale transaction. In applying the model to the underlying asset values the question on attributing the instability coefficient arises and a solution is proposed implying a revision within the pricing model for this purpose, also the necessary question of the appropriate rate is addressed.
Genetic basis of offspring number and body weight variation in Drosophila melanogaster

Drosophila melanogaster egg production, a proxy for fecundity, is an extensively studied life-history trait with a strong genetic basis. As eggs develop into larvae and adults, space and resource constraints can put pressure on the developing offspring, leading to a decrease in viability, body size, and lifespan. Our goal was to map the genetic basis of offspring number and weight under the restriction of a standard laboratory vial. We screened 143 lines from the Drosophila Genetic Reference Panel for offspring numbers and weights to create an "offspring index" that captured the number vs. weight trade-off. We found 30 associated variants in 18 genes. Validation of hid, Sox21b, CG8312, and mub candidate genes using gene disruption mutants demonstrated a role in adult stage viability, while mutations in Ih and Rbp increased offspring number and increased weight, respectively. The polygenic basis of offspring number and weight, with many variants of small effect, as well as the involvement of genes with varied functional roles, support the notion of Fisher9s "infinitesimal model" for this life-history trait.
A Fresh Start for Stigmatized Groups: The Effect of Cultural Identity Mindset Framing in Brand Advertising

The idea that individuals can create a new beginning, known as the fresh start mindset, is deeply embedded in American culture. This mindset represents an accessible, shared construct that may be p...
Abstract 2338: The GLS1 inhibitor IPN60090 enhances antitumor immune response through metabolic reprogramming of T cells and impacts on the tumor microenvironment

Therapeutic agents targeting metabolism in the tumor microenvironment have been of increasing interest in recent years, however, the complexities of the interplay between tumor, stromal and immune cell interactions add complexity to these therapeutic approaches. We have previously disclosed the development of the GLS1 inhibitor, IPN60090, which is currently progressing through Phase 1 studies (NCT03894540) in multiple indication-specific biomarker-positive patient populations. In order to fully appreciate the opportunity to enhance IPN60090 activity in patients, we explored the impact of GLS1 inhibition on the activity of the immune system. Glutamine metabolism has been shown to play important and varied roles within the immune compartment including, but not limited to, roles in T-cell activation, T-cell effector functions and progression of exhaustion phenotypes. During T-cell activation, glutamine is utilized to drive activated T-cells towards a glycolytic phenotype. Eventually, activated T-cells exhaust and shift away from glycolysis towards fatty acid oxidation. Interestingly, it has been reported that the interaction of PD1 with PD-L1 blocks glutamine import and decreases glycolysis. Given these data, we hypothesized that inhibition of GLS1 with IPN60090 might enhance checkpoint blockade by increasing levels of glutamine in the tumor microenvironment, thus enhancing anti-tumor immune responses. In ex vivo culture systems, we show that IPN60090-mediated GLS1 inhibition increases the glycolytic activity of CD4+ and CD8+ T-cells, suggesting that GLS1 inhibition allows cells to maintain an energetically favorable phenotype. Furthermore, we show that IPN60090 enhances checkpoint blockade through cooperation with αPD1 therapy in two syngeneic mouse models which do not harbor predictive biomarkers of response to IPN60090 and which are refractory to checkpoint blockade. The observed synergy is due in part to IPN60090-dependent depletion of regulatory T-cells (Treg) and a concurrent increase in the CD8+ T-cell to Treg ratio in the tumor microenvironment. These data suggest that IPN60090 may show clinical benefit by enhancing immune response in the context of checkpoint blockade and have served as the justification for phase 1b trials in combination with Pembrolizumab which will enroll in 2021. Citation Format: Erika Suzuki, Jennifer Molina, Nakia D. Spencer, Christopher A. Bristow, Angela L. Harris, Ningping Feng, Mikhila Mahendra, Sonal Gera, Michael J. Soth, Kang Le, Timothy A. Yap, Giulio Draetta, Philip Jones, Timothy P. Heffernan, Jeffrey J. Kovacs. The GLS1 inhibitor IPN60090 enhances antitumor immune response through metabolic reprogramming of T cells and impacts on the tumor microenvironment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2338.
Common Surgical Procedures in Camelids

Veterinarians are commonly asked to perform surgery on llamas and alpacas. Several common surgical procedures are described including castration, gastrointestinal surgery, cesarean section, angular limb deformity and tooth-root abscess treatment.
Copyright at the Museum: Using the Publication Doctrine to Free Art and History

This Article is the first to use the copyright publication doctrine to clarify whether art, photographs, films, and historical documents that fill our museums and libraries are in the public domain. Knowing whether a photo, painting, film, or original letter was published is critically important to anyone who wants to use it today. Before 1989, publishing a work with no copyright notice dedicated the work to the public domain. Unpublished works without a notice are likely protected by copyright, and their unauthorized use can result in severe federal penalties. Unfortunately, the meaning of “publication” in copyright law is notoriously ambiguous. The federal statutory definition suggests that works “made available” to the public are published, while leading treatises generally assume that works given to public museums and libraries are unpublished. Confronted with this uncertainty, risk averse institutions too often assume that archived works are protected by copyright. Misunderstanding the law can keep cultural treasures locked in dark archives, vaults and basements, preventing their use as a foundation for new expression and distorting our sense of history.This Article critically examines mistaken assumptions about copyright publication. It finds that neither the statutory definition nor leading treatises adequately identify when a work is published. A better standard for determining when a work is published and in the public domain is needed to free works from being locked up by copyright uncertainty. The best solution would clarify the boundaries of a stable public domain. In a recent decision, the Supreme Court took a wrong turn in dismissing the importance of the public domain. Knowing what content may be freely used is critical to preserving First Amendment values and freeing cultural treasures from copyright’s bondage. The copyright ambiguity of archived works should be resolved in a way that honors the expressive and historical value of the public domain. After considering several alternatives, this Article shows how precedential patterns point to the best solution to the publication ambiguity. Drawing on empirical analysis of federal cases interpreting copyright publication, I identify the variables that are most important in determining whether archived works are published. The suggested solution focuses on copyright owner intent and the availability of authorized copies. Other factors described as significant in leading treatises — such as the type of work or archive — actually mask these two fundamental inquiries. The proposed standard provides a much needed solution to clarify which pieces of our cultural heritage are in the public domain and freely available as raw materials for educational sharing, expressive work, historical research, and public discourse.
Marleda: effective distribution estimation through markov random fields

Many problems within the biological sciences, such as DNA sequencing, protein structure prediction, and molecular docking, are being approached computationally. These problems require sophisticated solution methods that understand the complex natures of biological domains. Traditionally, such solution methods are problem specific, but recent advances in generic problem-solvers furnish hope for a new breed of computational tools. The challenge is to develop methods that can automatically learn or acquire an understanding of a complex problem domain. Estimation of Distribution Algorithms (EDAs) are generic search methods that use statistical models to learn the structure of a problem domain. EDAs have been successfully applied to many difficult search problems, such as circuit design, optimizing Ising spin glasses, and various scheduling tasks. However, current EDAs contain ad hoc limitations that reduce their capacity to solve hard problems. This dissertation presents a new EDA method, the Markovian Learning Estimation of Distribution Algorithm (MARLEDA), that employs a Markov random field model. The model is learned in a novel way that overcomes previous ad hoc limitations. MARLEDA is shown to perform well on standard benchmark search tasks. A multiobjective extension of MARLEDA is developed for use in predicting the secondary structure of RNA molecules. The extension is shown to produce high-quality predictions in comparison with several contemporary methods, laying the groundwork for a new computational tool for RNA researchers.
Airport Concentration and Market Power: An Events Study Approach

This paper presents independent confirmation of the results obtained in recent studies that suggest mergers and acquisitions creating airline dominance at the airport level lead to market power. Using a different methodology - an events study for the 1986 merger of Northwest Orient Airlines and Republic Airlines - this paper confirms those results, indicating that concentration in the context of the sunk costs associated with the operation at a particular airport facility allows market power in the airline industry.
GENERALIZED GROUP CHARACTERS AND COMPLEX ORIENTED COHOMOLOGY THEORIES

Let BG be the classifying space of a finite group G. Given a multiplicative cohomology theory E ⁄ , the assignment G 7i! E ⁄ (BG) is a functor from groups to rings, endowed with induction (transfer) maps. In this paper we investigate these functors for complex oriented cohomology theories E ⁄ , using the theory of complex representations of finite groups as a model for what one would like to know. An analogue of Artin's Theorem is proved for all complex oriented E ⁄ : the abelian subgroups of G serve as a detecting family for E ⁄ (BG), modulo torsion dividing the order of G. When E ⁄ is a complete local ring, with residue field of characteristic p and associated formal group of height n, we construct a character ring of class functions that computes 1 E ⁄ (BG). The domain of the characters is Gn,p, the set of n-tuples of elements in G each of which has order a power of p. A formula for induction is also found. The ideas we use are related to the Lubin Tate theory of formal groups. The construction applies to many cohomology theories of current interest: completed versions of elliptic cohomology, E ⁄ n- theory, etc. The nth Morava K-theory Euler characteristic for BG is computed to be the number of G-orbits in Gn.p. For various groups G, including all symmetric groups, we prove that K(n)⁄(BG) concentrated in even degrees. Our results about E⁄(BG) extend to theorems about E⁄(EG◊GX), where X is a finite G-CW complex.
A two-stage autonomous evolutionary music composer

An autonomous music composition tool is developed using Genetic Algorithms. The composition is conducted in two Stages. The first Stage generates and identifies musically sound patterns (motifs). In the second Stage, methods to combine different generated motifs and their transpositions are applied. These combinations are evaluated and as a result, musically fit phrases are generated. Four musical phrases are generated at the end of each program run. The generated music pieces will be translated into Guido Music Notation (GMN) and alternate representation in Musical Instrument Digital Interface (MIDI). The Autonomous Evolutionary Music Composer (AEMC) was able to create interesting pieces of music that were both innovative and musically sound.
Chapter 36 Restless legs syndrome and periodic limb movements

Publisher Summary Restless legs syndrome (RLS) and periodic limb movements (PLM) have been linked since the 1960s when Lugaresi, and his colleagues discovered that most patients with RLS suffer from periodic limb movements in sleep (PLMS). While highly associated, however, they do not appear to be merely alternate forms of one central neural process. Either can exist in a person without the other. Evidence suggests that RLS is only the most common condition associated with PLM. As recently defined by a consensus conference at the NIH held in conjunction with the International RLS Study group (IRLSSG), RLS is a clinical condition in which cognitively intact adult patients complain of four key symptoms. In brief, these are: (1) an urge to move, associated with abnormal sensation; (2) induction or aggravation by rest; (3) relief with activity; and (4) intensification at later phases of the normal day/night cycle (evening and night). While these symptoms are necessary to define RLS, there are mimics such as leg cramps or some polyneuropathic discomforts that can also satisfy these criteria and must be separately distinguished from RLS.
The Structure and Properties of the Cell Surface Coat

Publisher Summary This chapter discusses the structure and properties of the cell surface coat. Cell surface coats have been found on all living cells that have been examined with sufficiently sensitive methods. These cells protect their fragile plasma membrane with a relatively strong, hydrophilic coat which is a permanent feature of the cell surface complex. Under special conditions, however, rarely and momentarily, these coats can be breached or even removed from certain cells to reveal the delicate, oily, liquid like layer of the plasma membrane, damage to which is lethal unless immediately repaired. These cells usually are able to replace their missing coats in a matter of minutes. Most animal cell coats appear in the electron microscope (EM) as a uniform layer at the external cell surface. The layer seems to originate at, or be a part of, the outer leaflet of the trilaminar plasma membrane, and usually is 100-200 A thick. A variety of methods has been developed to reveal the ultrastructure of cell coats, the details of which depend on the particular method employed. The strength or durability of the cell surface coat varies dramatically among various cells.
Metacognition in web-based learning activities

One of the main challenges for those working in the field of ICT mediated learning is to develop innovative systems to support the knowledge building process. [email protected], a system developed in the Institute for Educational Technology, is an attempt to meet this challenge. It is a new educational tool for middle schools which stimulates awareness of students' cognitive and learning strategies by asking metacognitive questions during hypermedia web surfing. This paper presents the features of the system, the pilot study carried out with pupils, the results, and the suggestions for further developments.
Improved purification and characterization of hemolysin BL, a hemolytic dermonecrotic vascular permeability factor from Bacillus cereus

Bacillus cereus causes diarrheal and emetic food poisoning syndromes as well as a variety of mild to severe infections. A dermonecrotic vascular permeability (VP) factor has been implicated as a virulence factor in these illnesses. Hemolysin BL was previously identified as a unique tripartite hemolysin possessing VP activity. In this study, a high-yield purification scheme, which allowed quantitative characterization of hemolysin BL activity and determination of the molecular weight, pI, and N-terminal sequence of each component, was developed. Milligram quantities of the B, L1, and L2 components were highly purified by a combination of anion-exchange and hydroxylapatite chromatographies. The combined components had VP activity at low doses and were necrotic at higher doses. The toxin exhibited an unusual dose-response zone phenomenon in turbidometric hemolysis assays. Activity increased at doses up to 200 ng/ml, then decreased at doses up to 350 ng/ml, and was constant at doses up to at least 2,500 ng/ml. This behavior may provide an explanation for the unusual discontinuous pattern typical of hemolysin BL in gel diffusion assays. At high concentrations of one or two components, the presence of low amounts of the complementary component(s) resulted in full hemolytic activity. Erythrocytes were protected from lysis by Zn2+ at micromolar concentrations but not by Ca2+ and Mg2+ at concentrations up to 25 mM. These data provide guidelines for future work on this toxin and indicate that hemolysin BL is the dermonecrotic VP factor implicated as a B. cereus virulence factor.
Evaluating diagnostic tests.

The presentations at this conference will discuss new technologies and rapid scientific developments that have resulted in new diagnostic tests for periodontal disease, musculoskeletal imaging, temporomandibular joint dysfunctions, and incipient coronal and root dental caries. However, for many of these claims, there has been insufficient scientific support regarding the sensitivity and specificity of the tests, or their ability to predict the percent of cases in which the disease or condition progresses to the next state of development.Research is needed that will yield the basic diagnostic parameters of new diagnostic tests, i.e., their accuracy, precision, sensitivity, specificity, positive predictive value, and negative predictive value. The purpose and methods for calculating each of these measures are described in this paper. Five questions are then presented that will need to be addressed in future research regarding new diagnostic tests: (1) Does the scientific theory of the test fit with our curr...
High-resolution satellite image fusion using regression kriging

Image fusion is an important component of digital image processing and quantitative image analysis. Image fusion is the technique of integrating and merging information from different remote sensors to achieve refined or improved data. A number of fusion algorithms have been developed in the past two decades, and most of these methods are efficient for applications especially for same-sensor and single-date images. However, colour distortion is a common problem for multi-sensor or multi-date image fusion. In this study, a new image fusion method of regression kriging is presented. Regression kriging takes consideration of correlation between response variable (i.e., the image to be fused) and predictor variables (i.e., the image with finer spatial resolutions), spatial autocorrelation among pixels in the predictor images, and the unbiased estimation with minimized variance. Regression kriging is applied to fuse multi-temporal (e.g., Ikonos, QuickBird, and OrbView-3) images. The significant properties of image fusion using regression kriging are spectral preservation and relatively simple procedures. The qualitative assessments indicate that there is no apparent colour distortion in the fused images that coincides with the quantitative checks, which show that the fused images are highly correlated with the initial data and the per-pixel differences are too small to be considered as significant errors. Besides a basic comparison of image fusion between a wavelet based approach and regression kriging, general comparisons with other published fusion algorithms indicate that regression kriging is comparable with other sophisticated techniques for multi-sensor and multi-date image fusion.
Optimization of circuit trajectories: an auxiliary network approach

On optimizing circuit trajectories, i.e. continuous paths of circuit parameters, the paper presents an auxiliary network approach, which utilizes Pontryagin's minimum principle. Based on a set of circuit element correspondence rules, the introduced approach establishes an auxiliary network for a given circuit to be optimized, and then circuit trajectories are optimized in a process of simulating the given circuit and the auxiliary network. The auxiliary network approach facilitates establishing analytic models in designing high-performance circuits that require fine tuning circuit trajectories. The paper details the theoretical framework of auxiliary network, and provides practical examples of its application in adiabatic circuit design.
Strong exciton-plasmon coupling in semiconducting carbon nanotubes

We study theoretically the interactions of excitonic states with surface electromagnetic modes of small-diameter $(\ensuremath{\lesssim}1\text{ }\text{nm})$ semiconducting single-walled carbon nanotubes. We show that these interactions can result in strong exciton-surface-plasmon coupling. The exciton absorption line shape exhibits Rabi splitting $\ensuremath{\sim}0.1\text{ }\text{eV}$ as the exciton energy is tuned to the nearest interband surface-plasmon resonance of the nanotube. We also show that the quantum confined Stark effect may be used as a tool to control the exciton binding energy and the nanotube band gap in carbon nanotubes in order, e.g., to bring the exciton total energy in resonance with the nearest interband plasmon mode. The exciton-plasmon Rabi splitting we predict here for an individual carbon nanotube is close in its magnitude to that previously reported for hybrid plasmonic nanostructures artificially fabricated of organic semiconductors on metallic films. We expect this effect to open up paths to new tunable optoelectronic device applications of semiconducting carbon nanotubes.
Homocysteine impairs coronary microvascular dilator function in humans

Abstract Objectives We sought to use positron emission tomography (PET) to test the hypothesis that hyperhomocysteinemia adversely effects coronary microvascular dilator function. Background Hyperhomocysteinemia is associated with abnormal endothelium-dependent vasodilation in peripheral human arteries. However, its effect on the coronary circulation is not known. Methods Eighteen healthy humans, age 24 to 56 years, were enrolled in a double-blind, crossover trial. Basal and adenosine-stimulated myocardial blood flow (MBF) was determined by PET: after ingestion of placebo and after methionine-induced hyperhomocysteinemia. Further, brachial ultrasonography was used to assess flow-mediated vasodilation. Additionally, to assess the role of nitric oxide (NO) in adenosine-mediated vasodilation, the MBF response to adenosine was measured in the presence and absence of the NO synthase antagonist NG-monomethyl-l-arginine (l-NMMA) (0.3 mg/kg/min intravenously). Results Hyperhomocysteinemia resulted in a reduction in the MBF dose-response curve to adenosine (p Conclusions The data, therefore, support the hypothesis that acute hyperhomocysteinemia impairs microvascular dilation in the human coronary circulation as a result of reduced NO bioavailability.
The ionospheres and plasma tails of comets

The paper reviews the current state of knowledge about cometary plasma (type I) tails and ionospheres. Observational statistics for type I tails are examined along with spectroscopic observations of plasma tails, identified ion species in such tails, and the morphology of cometary plasma tails and ionospheres. Evidence for a strong interaction between comets and the solar wind is evaluated on the basis of observations of plasma-tail orientations, large accelerations of tail structures, and correlations between disturbances in type I tails and solar-wind or geomagnetic disturbances. The use of comets as solar-wind probes is discussed, the nature of comet-solar-wind interactions is investigated, and ionization sources for cometary gases are considered. Hydrodynamic models of comet-solar-wind interaction are summarized, and the structure and ion chemistry of cometary ionospheres are studied. Observations suggesting that significant magnetic fields are associated with comets are briefly reviewed and interpreted.
Enx (Hox11L1)-deficient mice develop myenteric neuronal hyperplasia and megacolon

The isolated homeobox gene Enx (Hox11L1) is expressed in enteric neurons innervating distal ileum, and proximal and distal colon. Enx-deficient mice develop megacolon with massive distension of the proximal colon. The number of myenteric ganglia, total neurons per ganglion, and NADPH diaphorase presumptive inhibitory neurons per ganglion are increased in the proximal and distal colon, but decreased in the distal ileum of all Enx -/- mice. Enx -/- mice provide a model for human neuronal intestinal dysplasia (NID), in which myenteric neuronal hyperplasia and megacolon are seen. These results suggest that Enx is required for the proper positional specification and differentiate cell fate of enteric neurons.
An analysis of a satellite multibeam altimeter

Since the GEOS-3 and SEASAT-1 radar altimeters measured altitude over a narrow swath along the satellite subtrack, ocean current and mesoscale feature maps could only be generated after a large number of satellite revolutions. The present paper analyzes a new multibeam altimeter technique that has the potential to cover wide swaths. The multibeam altimeter uses two antenna elements, simple parabolic dishes with offset feeds, deployed cross-track on singly hinged booms into a fixed measurement geometry to generate an interferometer pattern over the desired swath extent. Range gating allows the isolation of a single interferometeric lobe, and the desired altitude measurements are extracted by using a modified altitude tracker design. Implementing this sensor on future altimetry missions would allow the timely generation of ocean current and mesoscale feature maps for the first time.
Secular and Periodic Motions of the Node of an Artificial Earth-Satellite

IN a recent paper on the effect of the Earth's oblateness on the orbit of a near satellite, King-Hele1 mars an otherwise commendable presentation by abundant criticism of some earlier papers on this subject by myself and collaborators2,3. Although King-Hele's remarks were not so intended, his statements have had the effect of raising serious doubts about the validity of my early solutions and in particular about the usefulness of my approach to the problem of satellite orbit perturbations.
Characterization of GABA- and glycine-induced currents of solitary rodent retinal ganglion cells in culture.

Ganglion cells were fluorescently labeled, dissociated from 7- to 11-day-old rodent retinas, and placed in tissue culture. Whole-cell recordings with patch electrodes were obtained from solitary cells lacking processes, which permitted a high-quality space clamp. Both GABA (1-200 microM) and glycine (10-300 microM) produced large increases in membrane conductance in virtually every ganglion cell tested, including ganglion cells from different size classes in both rats and mice. Taurine evoked responses similar to those of glycine, but considerably greater concentrations of taurine (150-300 microM) were necessary to observe any effect. Since 20 microM GABA produced approximately the same response as 100 microM glycine, the effects of these two concentrations were compared under various conditions. When recording with chloride distributed equally across the membrane, the reversal potential of the agonist-induced currents was approximately 0 mV. When the internal chloride was reduced by substitution with aspartate, the reversal potential shifted in a negative direction by about 42 mV, indicating that the current was carried mainly by chloride ions. Strychnine (1-5 microM) completely and reversibly blocked the actions of glycine (100 microM) but not those of GABA (20 microM); however, higher concentrations of strychnine (20 microM) nearly totally inhibited the current elicited by GABA (20 microM). The responses to glycine (100 microM) were not affected by bicuculline methiodide (20 microM) or picrotoxinin (20 microM). In contrast, bicuculline methiodide (10 microM) and picrotoxinin (10 microM) reversibly blocked the current evoked by GABA (20 microM); d-tubocurarine (100 microM) only slightly decreased the response to GABA (20 microM). The antagonists were effective over a wide range of holding potentials (-90 mV to +30 mV). The responses to a steady application of both GABA and glycine decayed in a few seconds when recorded under conditions of both symmetric and asymmetric chloride across the membrane. During this decay the current and conductance decreased simultaneously, reflecting receptor desensitization rather than a change in the driving force for chloride caused by agonist-induced ionic fluxes. The time-course of desensitization was usually described by a single exponential with time constants for GABA (20 microM) and glycine (100 microM) of 4.0 +/- 1.6 s and 4.4 +/- 1.9 s (mean +/- S.D.), respectively.(ABSTRACT TRUNCATED AT 400 WORDS)
Augmentation of antitumor immune responses after adoptive transfer of bone marrow derived from donors immunized with tumor lysate-pulsed dendritic cells

Abstract We demonstrated previously that tumor lysate-pulsed dendritic cells (TP-DC) could mediate a specific and long-lasting antitumor immune response against a weakly immunogenic breast tumor during early lymphoid reconstitution. The purpose of this study was to examine the potential therapeutic efficacy of bone marrow transplants from TP-DC-vaccinated donors. In 2 aggressive metastatic models, bone marrow transplantation with donor bone marrow cells from TP-DC-immunized mice mediated a tumor-specific immune response in the recipient, and this caused regressions of preexisting tumor metastases. After vaccination with TP-DC, donors harbored increased numbers of both activated CD4+ and CD8+ T-cell populations in the bone marrow. Adoptive transfer of T cells purified from the bone marrow of TP-DC-vaccinated mice led to a reduction in preestablished lung metastases, whereas depletion of T cells from bone marrow abolished this effect. By using T cells derived from the bone marrow of TP-DC-vaccinated major histocompatibility complex class I and class II knockout mice, the effector cells required for the observed antitumor effect were determined to be major histocompatibility complex class I-restricted CD8+ T cells. Additionally, the tumor burden in TP-DC-immunized transplant recipients could be reduced further by repetitive TP-DC immunizations after bone marrow transplantation. Collectively, these results demonstrate an important therapeutic role of bone marrow from TP-DC-immunized donors and raise the potential for this approach in patients with advanced cancer.
Determination of the inter-spike times of neurons receiving randomly arriving post-synaptik potentials

Stein's model for a neuron receiving randomly arriving post-synaptic potentials is studied from an analytic viewpoint, using some recent results in the theory of first passage times for temporally homogeneous Markov processes. The case when the only input is excitatory can be treated exactly. It is shown that the moments of the firing time are guaranteed to be finite so that the differential-difference equation for the expectation (and higher moments) of the time for the membrane potential to first reach threshold from resting level can be written down. Analytic solutions are obtained in a number of cases with main emphasis on the case when the threshold is twice the epsp magnitude. An invariance principie is formulated wherein at a given mean input frequency and for a given decay parameter, the distribution of firing times depends only on the ratio of threshld to epsp magnitude. For the case where this ratio is two, the variation in the mean discharge rate is obtained as a function of mean input frequency. The results are compared with the experimental data for the Poisson monosynaptic excitation of cat motoneurons by Redmanet al. Agreement between theoretical and experimental values is excellent at input frequencies near 102 sec-1, and theory underestimates the firing rate below that input frequency. Reasons for the discrepancy are discussed at length including the uncertainties in the neuronal parameters and the dependence of epsp magnitude on mean input frequency. The problem of including an inhibitory input process together with excitation is treated by an approximation procedure when the inhibition is considerably weaker than the excitation. At the input frequency investigated it is shown that when inhibition "half as weak" as the excitation occurs, the mean discharge frequency is approximately halved. In the final section a method of estimating neuronal parameters from the moments of the experimental inter-spike time distribution is outlined.
Laboratory measurement of absorption by ammonia around 5 microns

Abstract Infrared absorption by ammonia has been measured between 2000 and 2200 cm-1 using samples of the gas diluted by hydrogen, helium and nitrogen. Data on absorption coeffiecients have been obtained employing path lengths up to ten meters and total pressures up to 8 atm. In the three cases of broadening studied, it is apparent that the measured absorption is neither due to the far wings of lines in the bands surrounding the region nor due to any dimer absorption. Thus, the observed absorption should be totally accountable by absorption due to local lines in the region.
Myocardial Blood Flow and Myocardial Uptake of 201Tl and 99mTc-Sestamibi During Coronary Vasodilation Induced by CGS-21680, a Selective Adenosine A2A Receptor Agonist

Background —We investigated the hemodynamic and coronary vasodilatory effects of CGS-21680, a potent selective adenosine A 2A agonist, as well as its potential use as a new stress modality in combination with perfusion scintigraphy. Methods and Results —A stenosis of the left anterior descending coronary artery (LAD) was produced in dogs to reduce the reactive hyperemic response to 201 Tl (0.5 mCi) and 99m Tc-sestamibi (5 mCi) were injected at the maximal dose of CGS-21680. Heart rate decreased with adenosine but increased during CGS-21680 infusion ( P P P P P =NS), and transmural 201 Tl and 99m Tc-sestamibi count-activity ratios (0.48±0.11 and 0.51±0.09, respectively) were also comparable ( P =NS). Myocardial scintigraphy uncovered perfusion defects in all dogs. Conclusions —CGS-21680 elicits coronary vasodilation comparable to that of adenosine and produces profound heterogeneity of MBF and of 201 Tl and 99m Tc-sestamibi myocardial uptake, rendering it a promising agent for pharmacological myocardial perfusion imaging.
Multicommodity Eulerian-Lagrangian Large-Capacity Cell Transmission Model for En Route Traffic

A new paradigm for building an Eulerian-Lagrangian cell transmission model for air traffic flow is developed. It is based on an aggregation of track data and is applied to the full National Airspace System in the United States. The Eulerian-Lagrangian model is based on a multicommodity network flow model constructed from historical air traffic data. The flow model is reduced to a linear time invariant dynamical system, in which the state is a vector of aggregate aircraft counts. This model is called a large-capacity cell transmission model in reference to the cell transmission model in highway traffic, which inspired this work. The predictive capabilities of the model are successfully validated against recorded air traffic data by showing an accurate match between predicted sector counts (based on filed flight plans) and measured sector counts. The problem of controlling the sector aircraft count is posed as an integer program in which the dynamical system appears in the constraints. To improve the computational time for solving the problem, the integer program is relaxed to a linear program. The computational results show that a high portion of solutions of the linear program are integers, making this method appropriate for high-level traffic flow management.
Modeling multi-axial deformation of planar anisotropic elasto-plastic materials, part I: Theory

Abstract In sheet metal forming processes local material points can experience multi-axial and multi-path loadings. Under such loading conditions, conventional phenomenological material formulations are not capable to predict the deformation behavior within satisfying accuracy. While micro-mechanical models have significantly improved the understanding of the deformation processes under such conditions, these models require large sets of material data to describe the micromechanical evolution and quite enormous computation expenses for industrial applications. To reduce the drawbacks of phenomenological material models under the multi-path loadings a new anisotropic elasto-plastic material formulation is suggested. The model enables the anisotropic yield surface to grow (isotropic hardening), translate (kinematic hardening) and rotate (rotation of the anisotropy axes) with respect to the deformation, while the shape of the yield surface remains essentially unchanged. Essentially, the model is formulated on the basis of an Armstrong–Frederick type kinematic hardening, the plastic spin theory for the reorientation of the symmetry axes of the anisotropic yield function, and additional terms coupling these expressions. The capability of the model is illustrated with multi-path loading simulations in ‘tension-shear’ and ‘reverse-shear’ to assess its performance with ‘cross’ hardening and ‘Bauschinger’ effects.
Modified Thiocarbohydrazide Procedure for Scanning Electron Microscopy: Routine use for Normal, Pathological, or Experimental Tissues

A modified thiocarbohydrazide (TCH) technique to cross-link osmium layers has resulted in a reliable method for preparing a wide variety of soft biological tissues for scanning electron microscopy without the use of evaporated metal. The technique works equally well on tissues with smooth surfaces and those with abrupt changes in contour or cut surfaces, as in biopsy or autopsied material or pathologically altered tissues, and thus has wide applicability. Small surface structures and functional areas between cells are distinct. In addition, thin cells such as alveolar endothelium in the lung exhibit a transparent property, allowing the visualization of cells within the capillary lumen while retaining adequate contrast for study of the capillary wall itself. Absence of an evaporated metal coat makes the removal of tissue from the scanning electron microscope specimen easy for embedding and examination of the same material with the transmission electron microscope.
Time Reversal and Reciprocity in Direct Reactions

Direct-reaction reciprocity tests of time-reversal invariance are analyzed on general grounds and specifically with the T-violating force derived in the preceding paper. A simple proof of Robson's theorem that a cut-off distorted wave Born approximation satisfies reciprocity irrespective of $T$ violation is indicated. Numerical calculations are described which show that Robson's theorem is not a severe restriction on direct-reaction reciprocity tests, and that the violation of reciprocity is of the same order of magnitude as the ratio of the $T$-odd to $T$-normal forces. If the $T$-violating term of the nucleon current contains an isovector part, the $T$-odd potential derived in the preceding paper implies roughly 0.2% violation of reciprocity in deuteron stripping. However, if the basic $T$ violation is isoscalar, the violation of reciprocity will be reduced by a factor of 50 in the context of the model considered.
Nanoscale optical spectroscopy and detection

We use a laser-irradiated metal tip to create a locally enhanced field at the tip apex. The tip acts as an optical antenna and is held a few nanometers above the sample surface so that a highly localized interaction between the enhanced field and the sample is achieved. The method has been successfully combined with vibrational spectroscopy by making use of the well-known effect of surface enhanced Raman scattering (SERS). We mapped out the vibrational modes of individual single-walled carbon nanotubes (SWNT) with a resolution down to l0nm.
Mechanisms of dislocation reduction in GaN using an intermediate temperature interlayer

A dramatic reduction of the dislocation density in GaN was obtained by insertion of a single thin interlayer grown at an intermediate temperature (IT-IL) after the initial growth at high temperature. A description of the growth process is presented with characterization results aimed at understanding the mechanisms of reduction in dislocation density. A large percentage of the threading dislocations present in the first GaN epilayer are found to bend near the interlayer and do not propagate into the top layer which grows at higher temperature in a lateral growth mode. TEM studies show that the mechanisms of dislocation reduction are identical to those described for the epitaxial lateral overgrowth process, however a notable difference is the absence of coalescence boundaries.