cssi/SciSciGPT-SciSciNet huggingface dataset:
Abstract 3077: TMPRSS2-ERG regulation of miRNA in prostate cancer.

Prostate cancer is the cause of the second highest number of cancer related deaths in men in the United States, with the highest number of new estimated cases every year. In addition, the survival rate in localized and regional prostate cancer is 100%, while metastatic prostate cancer is about 29%, with a wide spectrum of biological deregulations. MicroRNA (miRNA) is a small non-coding RNA, about 22 nucleotides long, that mostly targets the 3’UTR of a gene for translational repression or mRNA degradation. More recently, miRNAs have been shown to play an important role in tumorigenesis, cancer progression, and metastasis. MiR-200c is one of the miRNA widely studied in cancer, as it has a role in regulating epithelial-mesenchymal transition (EMT). One of the ways in which miR-200c inhibits EMT is through its ability to downregulate ZEB1, an EMT activator that primarily functions by repressing E-cadherin. ERG is a transcription factor that is often fused with TMPRSS2 in prostate cancer and promotes cancer progression. In our study, we investigated the interaction of ERG and miR-200c, two factors that play important role in cancer progression, in prostate cancer. First, we identified ERG regulated miRNAs through utilizing miRNA profiling in LNCaP cells that were overexpressing ERG and in VCaP cells that have ERG knocked down. We selected miRNAs that are repressed upon ERG overexpression and derepressed upon ERG knockdown and also overlapped the results with ERG ChIP-seq data to identify ERG regulated miRNAs. Through this process we discovered that miR-200c is a good ERG-targeted miRNA. To validate the bioinformatics results, we used ERG overexpression in LNCaP and ERG knockdown in VCaP to measure mature miR-200c levels by miRNA assay. In addition, ERG ChIP-qPCR and miR-200c promoter luciferase assays showed that ERG binds at the miR-200c promoter. Next, to identify functional roles of miR-200c in ERG overexpressed cells, we performed invasion and migration assays to show that miR-200c plays an important role in the inhibition of ERG-mediated cancer progression. This study identifies an important factor, miR-200c, whose loss mediates the progression of TMPRSS2:ERG fusion-positive prostate cancer. According to our results, it might be possible to use miR-200c as a future therapeutic for ERG positive patients. This work was supported in part by the Research Scholar Award RSG-12-085-01 (to J.Y.) from the American Cancer Society, Department of Defense PC080665 (to J.Y.) and NRSA pre-doctoral fellowship T32 CA080621 (to J.K.). Citation Format: Jung Kim, Longtao Wu, Hong-Jian Jin, Jonathan Zhao, Jindan Yu. TMPRSS2-ERG regulation of miRNA in prostate cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3077. doi:10.1158/1538-7445.AM2013-3077
What political science can contribute to the study of law

Despite an inherent kinship, the studies of political science and law spent many decades isolated from one another. In recent years the two fields have become more and more integrated, with an increasing number of political scientists collaborating with law professors and joining law school faculties. Political science is a rigorous discipline that can benefit both legal scholars and lawyers. Public Law—the subfield of political science that studies law and courts—has much to offer in understanding how judges make decisions and how larger political and institutional contexts affect the legal system. Furthermore, law students can only benefit from exposure to the methodological approaches that are standard in political science. Enhanced integration of political science and law will inherently expand the knowledge and reach of lawyers and legal scholars due to the important contributions discussed in this article.
Cold Aisle Air Distribution in a Raised Floor Data Center With Heterogeneous Opposing Orientation Racks

In this paper we experimentally investigate the air flow distribution at the inlets of two opposing racks in a cold aisle. The racks have non uniform heat load and air flow requirements creating a heterogeneous data center environment. The Computer Room Air Conditioning (CRAC) unit fan speed is set to meet the air requirement of the high heat density rack. The effect of perforated tile air velocity on air distribution to both the racks is studied using particle image velocimetry (PIV) technique. PIV images are recorded at various rack heights corresponding to the locations of the servers in the rack. The PIV images recorded at various locations are stitched to construct a complete rack inlet air flow map. Three cases of rack air flow distributions are studied by varying the server work load and perforated tile flow rate. A significant change in the air distribution pattern is observed for the three cases investigated.Copyright © 2011 by ASME
System designs and applications

Solid oxide fuel cells (SOFCs) have been developed for a broad spectrum of power generation applications. The attractive feature of the SOFC is its clean and efficient production of electricity from a variety of practical fuels. SOFC power systems considered ranging from watt-sized portable devices to multi-megawatt stationary power plants. The development of a functional architecture for an SOFC power system involves design, analysis and optimisation to define suitable system configurations and specify system components and operating parameters that meet the requirements of the target application. This chapter provides an overview of SOFC power system designs, discusses key design factors and operating parameters for SOFC stacks, reviews selected examples of system configurations for portable, transportation and stationary power applications and briefly discusses system designs for SOFCs operating in electrolysis mode for hydrogen/chemical production.
Sensitivity analysis for frequency reliability of a random bending vibration system of continuous beam

With a criterion that the absolute value of difference between natural frequency and exciting frequency is less than a prescribed value,the frequency reliability analysis method avoiding resonance was proposed using the mechanical dynamics and reliability theory here.Furthermore,the sensitivity analysis method for frequency reliability was presented.The expressions for frequency reliability sensitrivity when parameters were normally distributed random variables were derived and the relation between the reliability and the fluctuation of distribution parameters of random variables was obtained.Through studying the effect of varying design parameters on the system reliability,the sensitivity analysisi method for the resonance reliability of dynamic structures was explored.A random bending vibration system of continuous beam was taken as an example,the sensitivity analysis for its frequency reliability was performed.The theoretical calculation results agreed well with those of the engineering practice.Both of them indicated the effectiveness of the proposed method.
Vehicle Routing Optimization with Carbon Emissions Considered under Time-varying Network

For the vehicle scheduling and routing distribution problem in urban distribution,we provide a multi-objective model pertaining to minimization of travel time and carbon emissions,which treats the travel speed as a critical variable.And a multi-objective particle swarm optimization(MOPSO) algorithm is provided to solve the problem.Finally,a numerical experiment about "cargo of city" is conducted.The superiority of time-varying network is shown.Also the influence of customer satisfaction and vehicle capacity on the Pareto results is analyzed.The research results will play an important instructing role in theory on the urban distribution with carbon emissions considered.
Prevalence and risk factors of memory disorders among elderly patients with chronic obstructive pulmonary disease

Objective To investigate the prevalence and influencing factors of memory disorder among elderly pa-tients with chronic obstructive pulmonary disease(COPD) and to provide basis for the improvement of memory function of the patients. Methods Form December 2009 to December 2010,138 COPD patients aged 60-80 years were selected with cluster sampling and surveyed with Rivermead Behavioral Memory Test-Second Edition(RBMT-Ⅱ) for their memory function. Results All the patients had memory impairment. The results of univariate analyses showed that the relevant factors of memory impairment were age(χ2=50. 164,P=0. 000),education level(χ2=55. 771,P=0. 000),liv-ing conditions(χ2=9. 877,P=0. 043),smoking(χ2=11. 683,P=0. 003),alcohol drinking(χ2=7. 689,P=0. 021), physical exercise(χ2=12. 633,P=0. 013),time of oxygen inhalation every day(χ2=31. 728,P=0. 000),duration of the disease(χ2=39. 927,P=0. 004),use of hormones(χ2=6. 248,P=0. 044),aerosol inhalation(χ2=21. 027,P=0. 000), the impairment degree of lung function(χ2=13. 072、P=0. 011),and partial pressure of carbon dioxide(χ2=25. 355, P=0. 000). Multi-variable regression analyses showed that education level(t=-6. 234,P=0. 000),living conditions(t=-2. 352,P=0. 020),duration of the disease(t=-3. 929,P=0. 000),alcohol drinking(t=-3. 073,P=0. 003), daily oxygen inhalation time(t=2. 905,P=0. 004),pulmonary function(t=2. 687,P=0. 008),and partial pressure of carbon dioxide(t=-1. 987,P=0. 002) were significat factors related to memory impairment among the elderly pa-tients. Conclusion The incidence of memory impairment in elderly patients with COPD is high and relates to complex factors.
Health Effects of Combined Exposure to Noise and High Temperature

With the development of modern industries and technologies, various kinds of harmful occupational factors often exist in one duty. Four major outcomes are included in health effects of combined factors: a synergetic effect, an additive effect, an antagonistic effect and no interactive effect. Noise and high temperature often exist in the same environment as harmful occupational factors. What are the combined effects of noise and high temperature? Are there any interactions such as synergistic, additive,antagonistic or negligible effects on health between noise and high temperature? The answers were different in literature. Synergistic,additive, antagonistic or negligible effects on auditory system, cardiovascular system and nervous system were all reported, and negligible effects on respiratory system were reported when co-exposure to noise and high temperature. At the present time, different findings were resulted by various experimental and exposure methodological inadequacies. Our viewpoints are that further research will focus on the specified conditions and dose-response relationships in which noise and high temperature have interactions, the mechanisms and potential interventions of their combined effects, interactive effects of noise and high temperature on other systems such as digestive system and immune system, etc.
The application of quality control circle, that in improving the success rate of intravenous catheter puncture by nurse

Objective: The quality control circle(QCC) methods is applied to improve the success rate of intravenous catheter puncture by nurse in the department of neurology. Methods: Establish quality control circle group、Choose some themes of improving the success rate of intravenous catheter puncture by nurse、Analysis the failure reason of intravenous catheter puncture、and according to the ten movable QCC steps to plan, implement,confirmation,disposal,and carry out the relevant quality improvement. Results:Through some quality control circle activities,the success rate of intravenous catheter puncture by nurse is increased from 80.36% to 92.75%.Conclusion: Application of quality control circle in intravenous catheter puncture can improve the success rate of intravenous catheter puncture by nurse. Because, it make us find out the root cause puncture failure,Analysis the causes and develop countermeasures.
InP-based integrated optical devices - present and future -

InP-based optical devices have been playing key roles over 30 years in the evolution of optical communication systems from the time-division-multiplexing systems in 1980's to recent digital-coherent systems. The enhanced functionalities and operation speed of the devices together with the improved electronics have brought about drastic reduction of size and power consumption of transceivers and network equipment as well as their costs. Increasing demands for network traffic have also expanded the application fields of the optical devices from the conventional telecom regions to short-reach regions such as intra-datacenter communication which now occupies 75% of the total traffic, and introduction of new technologies is strongly desired in terms of higher integration, lower power consumption and lower cost. In addition to the applications to communication networks, intensive efforts are being carried out for exploiting new applications such as sensing in near-infrared or mid-infrared wavelength region by taking advantages of the established InP-based technologies.
Pass the Idea Please: The Relationship between Network Position, Direct Engagement, and Course Performance in MOOCs

Extant research suggests that learner engagement in discussion forums is positively correlated with learner performance. In this paper we investigate which types of forum engagement are most strongly associated with final performance in MOOC courses. In particular, we compare the correlation between course final grade and two types of learner engagement: direct measures, which count the number of interactions, and indirect measures, which capture learners position in a social network. We found that direct measures have stronger correlations with final grade. However, in preliminary analyses, we also found that course instructors score higher than learners on some indirect measures. We discuss the implications of these findings and our plans for developing the work further in the future.
Towards an understanding of early childhood mathematics education: A systematic review of the literature focusing on practicing and prospective teachers:

This article presents the results of a literature review spanning 15 years (2000–2015), identifying empirical research relating to early childhood mathematics education. A total of 1141 articles we...
Juárez Girls Rising: Transformative Education in Times of Dystopia by Claudia G. Cervantes-Soon: (2017) Minneapolis, MN: University of Minnesota Press, 322 pp.

In Juarez Girls Rising: Transformative Education in Times of Dystopia, Claudia Cervantes-Soon (2017) provides readers with an ethnographic account of the lives of 10 young women who attended Prepar...
Advanced Systems Concept for Autonomous Construction and Self-repair of Lunar Surface ISRU Structures

In-Situ Resource Utilization is viewed by most as the basis for a successful manned exploration and settlement of the solar system. It is as its name implies, the “living off the land” that is a necessity for any untethered manned activity. The goal of ISRU is to allow settlements on the Moon and Mars and beyond to live with minimal if any dependence on the shipment of raw materials from Earth. The existence of a broad spectrum of elements on the lunar surface and on Mars gives hope that in principle it is possible to build a sustainable infrastructure on both these bodies that is based on local resources.
Culture, Communication, and Conflict: A Review of the Global Virtual Team Literature

Global virtual teams (GVTs) are teams distributed across geographical and cultural boundaries which have become more prevalent as organizations become more global. GVTs are a foundation of an agile organizational structure poised to capitalize on opportunities around the globe. This introductory literature review will build upon previous reviews within the literature to summarize the existing published knowledge on three key determinants of GVT success: culture, communication, and conflict. Arguments for culture to be viewed as a primary and unique antecedent of GVT effectiveness, due to the influence it has on communication and conflict, will be presented. Culture provides an additional layer of complexity that must be overcome when measuring the effect of other determinants, but particularly communication and conflict, on GVT outcomes. Finally, limitations within the literature and the directions for future research are presented.
Quantitative Proteomic Analysis of Biological Processes and Responses of the Bacterium Desulfovibrio desulfuricans ND132 upon Deletion of Its Mercury Methylation Genes.

Recent studies of microbial mercury (Hg) methylation revealed a key gene pair, hgcAB, which is essential for methylmercury (MeHg) production in the environment. However, many aspects of the mechanism and biological processes underlying Hg methylation, as well as any additional physiological functions of the hgcAB genes, remain unknown. Here, quantitative proteomics are used to identify changes in potential functional processes related to hgcAB gene deletion in the Hg-methylating bacterium Desulfovibrio desulfuricans ND132. Global proteomics analyses indicate that the wild type and ΔhgcAB strains are similar with respect to the whole proteome and the identified number of proteins, but differ significantly in the abundance of specific proteins. The authors observe changes in the abundance of proteins related to the glycolysis pathway and one-carbon metabolism, suggesting that the hgcAB gene pair is linked to carbon metabolism. Unexpectedly, the authors find that the deletion of hgcAB significantly impacts a range of metal transport proteins, specifically membrane efflux pumps such as those associated with heavy metal copper (Cu) export, leading to decreased Cu uptake in the ΔhgcAB mutant. This observation indicates possible linkages between this set of proteins and metal homeostasis in the cell. However, hgcAB gene expression is not induced by Hg, as evidenced by similarly low abundance of HgcA and HgcB proteins in the absence or presence of Hg (500 nm). Taken together, these results suggest an apparent link between HgcAB, one-carbon metabolism, and metal homeostasis, thereby providing insights for further exploration of biochemical mechanisms and biological functions of microbial Hg methylation.
Mask Weighted Stft Ratios for Relative Transfer Function Estimation and ITS Application to Robust ASR

Deep learning based single-channel time-frequency (T-F) masking has shown considerable potential for beamforming and robust ASR. This paper proposes a simple but novel relative transfer function (RTF) estimation algorithm for microphone arrays, where the RTF between a reference signal and a non-reference signal at each frequency band is estimated as a weighted average of the ratios of the two STFT (short-time Fourier transform) coefficients of the speech-dominant T-F units. Similarly, the noise covariance matrix is estimated from noise-dominant T-F units. An MVDR beamformer is then constructed for robust ASR. Experiments on the two- and six-channel track of the CHiME-4 challenge show consistent improvement over a weighted delay-and-sum (WDAS) beamformer, a generalized eigenvector beamformer, a parameterized multi-channel Wiener filter, an MVDR beamformer based on conventional direction of arrival (DOA) estimation, and two MVDR beamformers both based on eigendecomposition.
Silicon Quantum Dot-Poly(methyl methacrylate) Nanocomposites with Reduced Light Scattering for Luminescent Solar Concentrators

Silicon quantum dots with indirect bandgap photoluminescence are promising luminophores for large-area luminescent solar concentrators (LSCs). However, if commercially viable devices are to be achi...
A Total Internal Reflection Photoconductive Switch

We demonstrate a novel illumination technique for extrinsic photoconductive switches based on the creation of a total internal reflection trap to increase the optical path length. The optimal geometry is a square substrate illuminated from one of the corners along the diagonal, ensuring that the total internal reflection condition is maintained after any reflection off the device lateral sides. The optical absorption uniformity throughout the device bulk was improved by designing the entry window to have a cylindrical shape and by using a square core optical fiber as an illumination source. The concept is experimentally validated on a vanadium-doped silicon carbide device that shows approximately four-fold improvement in responsivity compared to normal illumination.
Making ‘my’ problem ‘our’ problem: Warfare as collective action, and the role of leader manipulation

Abstract Warfare is a collective action problem, and groups often stand to benefit from the quick and coordinated action that leaders can provide. This basic principle is as true in modern political contexts as it has been across our evolutionary history, and there is growing evidence that leadership has evolved, in part, to solve such collective action problems. Despite the material and reproductive benefits of leadership for groups, leaders may also seek private gains at the expense of group interests. Drawing upon insights from social and evolutionary psychology, I explain how leaders solve collective action problems in warfare, but also how leaders manipulate audience preferences when their own interests do not align with group interests. Specifically, when leaders anticipate great private gain from foreign aggression while facing steep public resistance at home, leaders will misframe the conflict as defensive rather than offensive in nature. I provide an evolutionary analysis that explains why leaders exploit this framing specifically, and I identify the specific aspects of conflict framing that are most likely to be exploited toward this end.
The Course of Catatonia: A Case Report from Inpatient Consultation through Two Years of Outpatient Care

Abstract Catatonia is a neuropsychiatric syndrome that affects 1.5 - 2% of patients referred to psychiatry consultation-liaison (C-L) services. Catatonia is a manifestation of an underlying medical illness in approximately 20% of patients seen in the general medical hospital. Acute symptoms often respond to intravenous (IV) benzodiazepines or electroconvulsive therapy, but little is known about the course of catatonia after patients transition to the outpatient setting. We present a case of a 60-year-old man with history of atrial septal defect, coronary artery disease, heart failure, peripheral artery disease, hypertension, type II diabetes, gout, and no known psychiatric history who was admitted to a general medical unit for altered mental status. Our inpatient C-L service diagnosed him with catatonia, based on the presence of immobility, mutism, stereotypy, withdrawal, and automatic obedience. These symptoms initially responded well to lorazepam during hospitalization, but they had recurred when he presented to our outpatient psychiatry C-L clinic. His symptoms have persisted over the last two years despite numerous medication trials and extensive workup for underlying conditions. In this report, we highlight both the challenges we have faced and successful strategies employed in the longitudinal care of this patient with catatonia and comorbid complex medical issues.
Alkyne hydration using Pt(II) catalysts

Abstract A series of Pt(II) derivatives has been found to be quite effective as alkyne hydration catalysts. These are new and differ from many other hydration catalysts in that there is no need to add protonic acids to or as the media. They are effective for hydration of electron rich alkynes but not useful for electron deficient systems. Finally, using unsymmetrically substituted alkynes, slight regioselectivity is observed with the carbonyl moiety preferring the position closer to the bulky substituent. When substituents have heteroatoms such as oxygen or sulfur, a chelation control process seems to govern the regiochemistry and high selectivities may be obtained.
Synthesis of enantiomerically pure N-Fmoc-S-Mob-(S)-α-Amino-ε-mercaptohexanoic acid and its use in solid phase peptide synthesis

Abstract Enantiomerically pure N-Fmoc-S-Mob (S)-α-amino-e-mercaptohexanoic acid (Amh) was synthesized in an efficient three step process from (L)-lysine via nucleophilic displacement of the pyridinium salt. An efficient method for Fmoc protection without any racemization is described. Incorporation of this unnatural amino acid into peptides and its complete deprotection was accomplished in high yield using standard procedures.
Parametric analysis of a steady state equilibrium-based biomass gasification model for syngas and biochar production and heat generation

Abstract In this study, a comprehensive steady-state model of downdraft biomass gasification process was developed using Aspen Plus simulation software. All four major gasification zones: drying, pyrolysis, oxidation, and reduction were modeled separately. Oxidation and Reduction zones were modeled using the Gibbs free energy minimization method with restricting chemical equilibrium approach. The simulated model was compared with the results of a wood-fed downdraft and sawdust fed gasifier reported in the literature. A parametric analysis was performed to analyze the effect of the gasifying agent, gasification temperature (500–1000 °C), equivalence ratio (0.1–0.5), and moisture content (0–25%) on the performance of gasifier. Performance evaluation of gasifier for syngas production and heat generation was performed by determining the low heating value of syngas, cold gas efficiency and thermal efficiency. The results indicated that the Equivalence ratio (ER) is the most important factor in gasification. Changes in the ER shows a very significant variation in syngas composition. Moreover, the increase in cold gas and thermal efficiencies was also very prominent until 700 °C after which it becomes constant. Biochar production increases with a decrease in moisture content and equivalence ratio.
Toxicity of pelvic nodal radiation for localized prostate cancer.

External beam radiation therapy (EBRT) is a definitive treatment modality for patients with localized prostate cancer (1). The entire prostate is included within the treatment field for all patients receiving EBRT for prostate cancer. EBRT along with androgen deprivation therapy (ADT) is recommended for patients with unfavorable intermediate- or high-risk disease, with national guidelines recommending a consideration of pelvic nodal irradiation for this subset of higher risk patients (1).
Antifibrinolytic Drugs and Allogeneic Transfusion in Pediatric Multilevel Spine Surgery: A Propensity Score Matched Cohort Study.

STUDY DESIGN Observational cohort study with propensity score matching. OBJECTIVE Determine whether antifibrinolytic drug use is associated with decreased allogeneic blood transfusion in multilevel pediatric spine surgery. SUMMARY OF BACKGROUND DATA Antifibrinolytic drugs are commonly used in adult multilevel spine surgery to reduce blood loss and allogeneic transfusion; however, only small studies have examined their efficacy in pediatric patients having multilevel spine surgery. METHODS Pediatric patients who had posterior multilevel spine surgery between 2016 and 2017 were identified in the national surgery quality improvement program participant use file. Propensity score matching was used to reduce bias from confounding and the rate of intraoperative allogeneic transfusion was compared between patients who received antifibrinolytic drugs and those who did not. Secondary outcomes included intraoperative cell saver volume, postoperative allogeneic transfusion, massive intraoperative transfusion, and adverse events including venous thromboembolism and seizure. RESULTS A total of 6904 patients underwent posterior multilevel spine surgery during the study period and 83% received antifibrinolytics. The matched cohort included 604 patients. Antifibrinolytic use had no association with reduced intraoperative allogeneic transfusion: odds ratio (OR) = 0.71 (99% confidence interval [CI] = 0.40-1.26, P = 0.12) or cell saver volume, median volume = 114 mL (0, 250 mL) in antifibrinolytic group versus 100 mL (0, 246 mL) in control group, P = 0.04. There was also no association with reduced postoperative allogeneic transfusion OR = 1.23 (99% CI = 0.54-2.81, P = 0.52) or massive transfusion OR = 1.0 (99% CI = 0.34-2.92, P = 1.0). No patient in the matched cohort had a venous thromboembolism or seizure. CONCLUSION Antifibrinolytic drugs are commonly used in pediatric multilevel spine surgery in the United States, but no efficacy was demonstrated in our study. There were no venous thromboembolisms or seizures implying an excellent safety profile in pediatric patients. LEVEL OF EVIDENCE 3.
Cancer-associated fibroblasts: key determinants of tumor immunity and immunotherapy

Immune-targeted approaches are rapidly changing the therapeutic landscape for cancer. In spite of that, most patients show resistance or acquire resistance to these therapies. Increasing work describing the tumor microenvironment (TME) has highlighted this space as one of the key determinants in tumor immune response and immunotherapeutic success. Frequently overlooked within this space, cancer-associated fibroblasts (CAFs) within the TME have surfaced as an important dictator of the tumor immune response. Herein, we review recent advances in defining the role of CAF-immune cell interactions in solid tumors and prospects for targeting stroma to overcome resistance to immunotherapy.
Generation of recombinant human ScFv antibodies against Rabies virus

Objective To obtain recombinant human anti-rabies antibodies by antibody phage library approach.Methods A combinatorial ScFv antibody phage library was constructed using pHAL14 vector from the peripheral blood of volunteers after rabies immunization.After package by hyperphage,the ScFv phage library was panned and selected by ELISA and IFA with purified rabies virus aG strain.The specific antibody was converted to full human IgG antibody with the VH Express and VK Express cassettes.Affinity and neutralizing test were performed to verify the function of the IgG molecules.Results Six unique human ScFv antibodies specific for the glycoprotein of rabies virus were obtained by ELISA,IFA and DNA sequencing.The full human IgG antibodies with high affinity represented a high level neutralizing activity to rabies virus aG strain,but not CVS strain.Conclusion The obtained human anti-rabies antibodies are important for diagnosis and treatment of rabies virus infection. Key words: Rabies virus; Immunoglobulins, heavy-chain; Immunoglobulins, light-china; Phages
Hydrodynamic quantum field theory: the onset of particle motion and the form of the pilot wave

We consider the hydrodynamic quantum field theory proposed by Dagan and Bush, a model of quantum dynamics inspired by Louis de Broglie and informed by the hydrodynamic pilot-wave system discovered by Couder and Fort. According to this theory, a quantum particle has an internal vibration at twice the Compton frequency that generates disturbances in an ambient scalar field, the result being self-propulsion of the particle through a resonant interaction with its pilot-wave field. Particular attention is given here to providing theoretical rationale for the geometric form of the wave field generated by steady, rectilinear particle motion at a prescribed speed, where signatures of both the de Broglie and Compton wavelengths are generally evident. While focus is given to the one-dimensional geometry considered by Dagan and Bush, we also deduce the form of the pilot wave in two dimensions. We further consider the influence on the pilot-wave form of the details of the particle-induced wave generation, specifically the spatial extent and vibration frequency of the particle. Finally, guided by analogous theoretical descriptions of the hydrodynamic system, we recast the particle dynamics in terms of an integro-differential trajectory equation. Analysis of this equation in the non-relativistic limit reveals a critical wave-particle coupling parameter, above which the particle self-propels. Our results provide the foundation for subsequent theoretical investigations of hydrodynamic quantum field theory, including the stability analysis of various dynamical states.
Core EPAs in the Acting Internship: Early Outcomes from an Interdepartmental Experience.

The Core Entrustable Professional Activities for Entering Residency (Core EPAs) are clinical activities all interns should be able to perform on the first day of residency with indirect supervision. The acting (sub) internship (AI) rotation provides medical students the opportunity to be assessed on advanced Core EPAs. All fourth-year AI students were taught Core EPA skills and performed these clinical skills under direct supervision. Formative feedback and direct observation data were provided via required workplace-based assessments (WBAs). Supervising physicians rated learner performance using the Ottawa Clinic Assessment Tool (OCAT). WBA and pre-post student self-assessment data were analyzed to assess student performance and gauge curriculum efficacy. In the 2017–2018 academic year, 167 students completed two AI rotations at our institution. By their last WBA, 91.2% of students achieved a target OCAT supervisory scale rating for both patient handoffs and calling consults. Paired sample t tests of the student pre-post surveys showed statistically significant improvement in self-efficacy on key clinical functions of the EPAs. This study demonstrates that the AI rotation can be structured to include a Core EPA curriculum that can assess student performance utilizing WBAs of directly observed clinical skills. Our clinical outcomes data demonstrates that the majority of fourth-year medical students are capable of performing advanced Core EPAs at a level acceptable for intern year by the conclusion of their AI rotations. WBA data collected can also aid in ad hoc and longitudinal summative Core EPA entrustment decisions.
Perioperative Skeletal Muscle Fluctuations in High-Acuity Liver Transplantation.

BACKGROUND Frailty has been implicated as a negative predictor of Liver Transplant (LT) outcomes. However, an understanding of changes in patient muscle mass peri-LT, and their effect in high-acuity patients remains lacking. We examined the impact of perioperative muscle mass changes (ΔSMI) on high-acuity (MELD ≥35) LT recipients. MATERIALS AND METHODS Skeletal muscle index (SMI) was calculated using CT imaging. Patients were divided into two groups, based on severity of peri-operative SMI decrease. LT recipients with chronic end-stage liver disease, MELD ≥35, and abdominal CT ≤30 days prior, and 30-90 days post LT were included. [1011 adult LT recipients reviewed, 2012-2018]. RESULTS Of 1011 patients reviewed, 88 met inclusion criteria (median MELD 41.1). The median ΔSMI was -5.0 (-29.4 - +21.1 cm2/m2) (fig A). Patients were classified into two groups: ΔSMI -5.0 (median ΔSMI: -9.2, n = 44). Recipients with ΔSMI<-5.0 had higher pre-LT SMI (35.4 versus 31.2 cm2/m2, P <0.001) and lower post-LT SMI (26.0 versus 30.8 cm2/m2, P <0.001). The ΔSMI<-5.0 group had higher early allograft dysfunction (40.9 versus 20.5%, P = 0.037), and inferior patient and graft survival (P = 0.015, 0.017, respectively). Multivariate analysis identified ΔSMI<-5.0 (HR: 2.938, P = 0.048), long cold-ischemia time (≥9h, HR: 7.332, P = 0.008), HCV (HR: 5.614, p = 0.001), and tracheostomy after LT (HR:9.218, P <0.001) as negative prognostic factors for patient survival . CONCLUSIONS Progressive perioperative sarcopenic deterioration was associated with inferior patient and graft survival in high acuity LT. These findings may guide pre and post-operative patient care and rehabilitation efforts in this challenging patient population.
Dial-a-ride problem: mixed integer programming revisited and constraint programming proposed

The dial-a-ride problem with time windows is studied in this article. First, a new efficient mixed integer programming formulation is proposed by merging some of drop-off nodes into paired pick-up ...
The old media business in the new: ‘the Googlization of everything’ as the capitalization of digital consumption

Digital labor and its role in the profitability of digital media companies have received increasing attention from scholars. However, digital audience labor has not been analyzed or recognized as t...
Dietary Sodium Loading Increases Central Systolic Blood Pressure More in Middle-Aged Compared to Young Salt Resistant Adults

Excess dietary sodium intake has been linked to increased incidence of cardiovascular disease and the development of hypertension. We sought to determine the effect of short-term dietary sodium loa...
Superconductivity and magnetism in CuBiSO from first principles

${\text{Cu}}_{1\ensuremath{-}y}\text{BiSO}$ was recently reported to superconduct at $T\ensuremath{\approx}5.8\text{ }\text{K}$ at $y\ensuremath{\approx}0.15$. Band-structure calculations indicate that the stoichiometric CuBiSO is a band insulator. In this Rapid Communication, I show that the hole-doped (whether in the virtual crystal approximation or with actual Cu vacancies) CuBiSO is on the verge of a ferromagnetic instability (cf. Pd metal) and therefore a conventional superconductivity with ${T}_{c}\ensuremath{\sim}6\text{ }\text{K}$ is quite unlikely. Presumably, the hole-doped CuBiSO is another example of superconductivity mediated by spin fluctuations.
The Role of Occupational Self-Assessment in Client-Centered Practice in School-Based Settings

Recent changes in educational legislation and societal views of health and wellness have resulted in sweeping changes in occupational therapy services delivery in school-based settings. Occupational therapy practitioners are uniquely equipped to identify the influence of supports and barriers on participation in meaningful contexts. With an understanding of student perception of occupational capacity and interest, practitioners can design client-focused, occupation-based interventions that promote lifestyle sustainability and community participation. The purpose of this article is to explore the role of occupational self assessment to enhance therapeutic partnership and empower students to identify and prioritize occupational needs and goals.
Protease-mediated degradation of lignin peroxidase in liquid cultures of Phanerochaete chrysosporium.

The decline of lignin peroxidase (LiP) activity observed after day 6 in cultures of Phanerochaete chrysosporium was found to be correlated with the appearance of idiophasic extracellular protease activity. Daily addition of glucose started on day 6 resulted in low protease levels and in turn in stable LiP levels. Addition of cycloheximide to day 6 cultures resulted in virtually no change of LiP activity and extracellular protein and negligible levels of protease activity, indicating that this protease is synthesized de novo. LiP activity was found to be stable upon removal of the fungal pellets on day 6 and incubation of the extracellular fluid alone. An almost complete disappearance of LiP activity and LiP proteins and high levels of protease activity were observed upon incubation of 6-day extracellular fluid in the presence of fungal pellets. Moreover, incubation of crude or purified LiP isoenzymes with protease-rich extracellular fluid of day 11 or 11-day cell extracts resulted in a marked loss of activity. In contrast, incubation of crude LiP with boiled and clarified extracellular fluid of day 11 cultures resulted in virtually no loss of activity. These results indicate that protease-mediated degradation of LiP proteins is a major cause for the decay of LiP activity during late secondary metabolism in cultures of P. chrysosporium. Images
Casimir Friction I: Friction of a vacuum on a spinning dielectric

We introduce the concept of Casimir friction, i.e. friction due to quantum fluctuations. In this first article we describe the calculation of a constant torque, arising from the scattering of quantum fluctuations, on a dielectric rotating in an electromagnetic vacuum.
Myocardial serotonin exchange: negligible uptake by capillary endothelium

The extraction of serotonin from the blood during transorgan passage through the heart was studied using Langendorff-perfused rabbit hearts. Outflow dilution curves of 131I- or 125I-labeled albumin, [14C]sucrose, and [3H]serotonin injected simultaneously into the inflow were fitted with an axially distributed blood-tissue exchange model to examine the extraction process. The model fits of the albumin and sucrose outflow dilution curves were used to define flow heterogeneity, intravascular dispersion, capillary permeability, and the volume of the interstitial space, which reduced the degrees of freedom in fitting the model to the serotonin curves. Serotonin extractions, measured against albumin, during single transcapillary passage, ranged from 24 to 64%. The ratio of the capillary permeability-surface area products for serotonin and sucrose, based on the maximum instantaneous extraction, was 1.37 +/- 0.2 (n = 18), very close to the predicted value of 1.39, the ratio of free diffusion coefficients calculated from the molecular weights. This result shows that the observed uptake of serotonin can be accounted for solely on the basis of diffusion between endothelial cells into the interstitial space. Thus it appears that the permeability of the luminal surface of the endothelial cell is negligible in comparison to diffusion through the clefts between endothelial cells. In 18 sets of dilution curves, with and without receptor and transport blockers or competitors (ketanserin, desipramine, imipramine, serotonin), the extractions and estimates of the capillary permeability-surface area product were not reduced, nor were the volumes of distribution. The apparent absence of transporters and receptors in rabbit myocardial capillary endothelium contrasts with their known abundance in the pulmonary vasculature.
Anti-Lambda/Anti-Proton Ratios at the AGS

Anti-Lambda (A) cross-sections in heavy ion collisions are of interest because strangeness production is a potential signal for QGP formation.1–3
Numerical study and parameter estimation for double-negative metamaterials with Hilbert-curve inclusions

The electromagnetic wave interaction with metallic Hilbert-curve inclusions of the 3/sup rd/ order embedded in a homogenous isotropic host medium is numerically studied. The electric and magnetic polarizability tensors for this inclusion are analyzed and the Maxwell-Garnett and periodic mixing formulations are applied to this class of inclusions in order to obtain approximate values for the effective permittivity and permeability of the bulk media. In addition, the effective parameters of the bulk medium are also extracted from the S-parameters of a finite element method (FEM) waveguide simulation and the agreement between these various approaches is examined.
121. Targeted Gene Therapy in the Treatment of X-Linked Hyper IgM Syndrome

X-linked hyper-IgM syndrome results in absent IgG, IgA, IgE and normal/elevated IgM due to defects in the CD40 ligand gene. HSCT is the only curative modality, but it carries significant risks, suggesting the need for improved methods of treatment. TALENs or CRISPRs, combined with the effective delivery of a homologous donor sequence containing normal CD40L DNA, will allow for targeted integration and provide physiologic expression of the endogenous CD40L gene to provide long-term immune reconstitution. TALENs targeting the 5′ UTR of the CD40L gene lead to allelic disruption of up to 31%at the target locus inK562 cells. In order to evaluate the capacity for targeted integration of a cassette at the cut site, cells were electroporated with the TALEN pair and a donor molecule with a promoterless GFP reporter gene flanked by homology arms. Expression of the GFP reporter was evaluated in Jurkat cells, with up to 10 % detected by flow cytometry and increasing to 22 % upon PHA activation. In addition, CRISPRs targeting a patient-specific mutation in intron 3 achieved >50 % gene disruption in K562 cells. Co-electroporation with a donormodified to contain a unique restriction enzyme site demonstrated site-specific gene integration. Site-specific modification at CD40L is achievable and physiologic expression of the endogenous CD40L gene could provide a viable therapy for XHIM.
Plasma gelsolin improves lung host defense against pneumonia by enhancing macrophage NOS3 function

Plasma gelsolin (pGSN) functions as part of the “extracellular actin-scavenging system,” but its potential to improve host defense against infection has not been studied. In a mouse model of primar...
Rates of Obsessive Compulsive Disorder in first degree relatives of patients with trichotillomania: a research note.

To explore a possible relationship between trichotillomania, (TTM) (compulsive hair pulling) and Obsessive Compulsive Disorder (OCD), 65 out of 69 (94%) first degree relatives of 16 female probands with severe chronic TTM were compared with two control groups for OCD and for TTM. Three (19%) of the 16 TTM probands had at least one first degree relative with a lifetime history of OCD, and there was an age corrected rate of 6.4% of first degree relatives with OCD. No relative in control group (A) met criteria for OCD. There was a trend (Fishers exact p = .07, two tailed) for a higher rate (age corrected) of OCD in TTM families; these pilot data are consistent with the concept of a spectrum of obsessive compulsive disorders which includes TTM and other pathological grooming behaviours.
Effects of Salesperson Experience, Age, and Goal Setting on New Product Performance Trajectory: A Growth Curve Modeling Approach

Based on survey data from 314 industrial salespeople, this study sheds light on the growth trajectory of a new product in the first 15 months after its launch, a critical period of new product success. A growth curve model was estimated using SAS Proc Mixed and the findings indicate that new product performance grows nonlinearly during this 15-month time frame. More importantly, salesperson-level factors significantly influence the new product performance growth. Specifically, the salesperson experience has a positive impact, whereas age has a negative impact on new product growth trajectory. These findings underscore the importance of effective alignment of the sales force in selling new products.
Long-Range Magnetization Transfer between Uncoupled Nuclei by Dipole−Dipole Cross-Correlated Relaxation: A Precise Probe of β-Sheet Geometry in Proteins

Interference between dipolar interactions in covalently linked (13)C-(1)H and nonlinked (1)H-(1)H pairs can be used to generate antiphase magnetization between noncoupled spins. The buildup rate of such antiphase terms is highly sensitive to local geometry, in particular the interproton distance and the (13)C-(1)H-(1)H internuclear angle. These rates have been measured for opposing C(alpha)H(alpha) pairs in antiparallel beta-sheets in the third Igg-binding domain of protein G (GB3) and in HIV protease, complexed with the inhibitor DMP323. For GB3, good agreement with the 1.1-A crystal structure is found. However, this agreement rapidly deteriorates with decreasing resolution of the corresponding X-ray structure. For HIV protease, two separate crystal structures that differ by less than 0.2 A from one another exhibit lower agreement in their predicted cross-correlated relaxation rates relative to one another than is found between experimental rates and the average of the rates predicted for the two structures. These data indicate that quantitative measurement of these cross-correlated relaxation rates can provide highly accurate structural information in macromolecules.