Antibodies to tubular basement membrane elicited by stimulation with allogeneic kidney.

Antibodies to tubular basement membrane elicited by stimulation with allogeneic kidney. It has been shown that Brown-Norway (BN) rats injected with homologous tubular basement membrane (TBM) preparations develop TBM antibodies and tubulointerstitial nephritis (TIN). TBM antigen(s) under study was expressed in BN rats and (MAXX × BN)F 1 hybrids but was lacking in MAXX rats. (MAXX × BN)F 1 hybrid rats were injected two to five times at weekly intervals with 2 × 10 8 lymphoid cells from MAXX rats. Circulating TBM antibodies and linear deposits of IgG along the TBM of proximal convoluted tubules (PCT), but not interstitial cellular infiltration and tubular damage, were found in the rats that had received lymph node cells. Spleen cells, thymocytes, X-irradiated, or mitomycin-C treated lymph node cells failed to elicit the development of the circulating TBM antibody as well as of TBM immune deposits. In other experiments, (MAXX × BN)F 1 hybrids were parabiosed with either parent for 15 days. TBM antibodies were demonstrated in the sera of hybrid as well as MAXX partners 14 and 20 days after the beginning of parabiosis. Forty days after parabiosis, however, TBM antibodies were detectable only in the sera of the hybrids. Linear deposits of IgG were found in the TBM of all the hybrids, but the immune deposits were not associated with TIN. TBM antibody response was not observed in parabiosis of hybrids with BN or of BN with MAXX. Furthermore, this antibody response was observed in parabiosis of normal hybrids with anephric MAXX, but not of anephric hybrids with normal MAXX. The results of these studies indicate that an immune response to TBM alloantigens can be elicited by lymph node cell transfer or parabiosis. The most plausible explanation of this finding is that MAXX lymphoid cells circulating through the hybrid kidney are stimulated by the TBM alloantigen, or that the parental lymphoid cells induce a mild graft-versus-host reaction characterized by an allogeneic effect. Apparition d'anticorps antimembrane basale tubulaire (TBM) apres stimulation par un rein allogenique. Il a ete montre qu'apres injection de preparations homologues de TBM, les rats Brown-Norway (BN) developpent des anticorps anti-TBM et une nephrite tubulo-interstitial (NTI). Le ou les antigenes de TBM etudies existaient chez des rats BN et chez des hybrides (MAXX × BN)F 1 , mais non chez des rats MAXX. Deux a cinq fois, a une semaine d'intervalle, 12 × 10 8 cellules lymphoides ont ete injectees a des hybrides (MAXX × BN)F 1 . Chez les rats ayant recu les cellules lymphoides, nous avons trouve des anticorps anti-TBM circulants et des depots lineaires d'IgG le long de la TBM du tubulaires proximites convolutes (TCP), mais ni infiltration cellulaire interstitielle, ni lesion tubulaire. Des cellules spleniques, des thymocytes et des cellules ganglionnaires irradiees ou traitees par de la mitomycine C n'ont pas entraine le developpement d'anticorps anti-TBM circulants, ni de depots immuns le long de la TBM. Dans d'autres experiences, des hybrides (MAXX × BN)F 1 ont ete mis en parabiose avec l'un de leur parent pendant 15 jours. Nous avons trouve des anticorps anti-TBM dans le serum des hybrides et dans celui des partenaires MAXX, 14 et 20 jours apres le debut de la parabiose. Cependant, au bout de 40 jours de parabiose, des anticorps anti-TBM n'etaient detectables que dans le plasma des hybrides. Des depots lineaires d'IgG etaient trouves dans les TBM de tous les hybrides, mais n'etaient pas associes avec une NTI. Il n'a pas ete observe de production d'anticorps anti-TBM apres parabiose d'hybrides avec des rats BN ou de BN avec des MAXX. En outre, la production d'anticorps a ete observee apres parabiose d'hybrides normaux avec des MAXX anephriques, mais non l'inverse. Ces resultats indiquent qu'une reponse immune a des allo-antigenes de TBM peut etre induite par le transfert de cellules lymphoides ou par parabiose. L'explication la plus plausible de ce fait est que les cellules lymphoides de MAXX traversant le rein hybride sont stimulees par un allo-antigene de TBM, ou que les cellules lymphoides parentales induisent une reaction greffon-contre-hote moderee caracterisee par l'effet allogenique.