cssi/SciSciGPT-SciSciNet huggingface dataset:
Seismic Rehabilitation of Reinforced Concrete Frame Interior Beam-Column Joints with FRP Composites

An experimental research program is described regarding the use of externally applied carbon fiber-reinforced plastic (CFRP) jackets for seismic rehabilitation of reinforced concrete interior beam-column joints, which were designed for gravity loads. The joints had steel reinforcement details that are known to be inadequate by current seismic codes in terms of joint shear capacity due to the absence of transverse steel hoops and bond capacity of beam bottom steel reinforcing bars at the joint. Lap splicing of beam bottom steel reinforcement at the joint using externally applied longitudinal CFRP composite laminates is investigated. Improvement of joint shear capacity using diagonal CFRP composite laminates is another strengthening scheme employed. Concrete crack widths for the as-built specimens and the extent of CFRP delamination for the rehabilitated specimens at various drift ratios are reported. The test results indicate that CFRP jackets are an effective rehabilitation measure for improving the seismic performance of existing beam-column joints with inadequate seismic details in terms of increased joint shear strength and inelastic rotation capacity. In addition, CFRP laminates are effective rehabilitation measures for overcoming problems associated with beam bottom steel bars that have inadequate embedment into the beam-column joints.
BRCA1 expression in a large series of sporadic ovarian carcinomas: A Gynecologic Oncology Group study

BRCA1 is a tumor suppressor gene that, when mutated, is associated with the development of hereditary ovarian cancer. A role for BRCA1 in the pathoetiology of sporadic ovarian epithelial cancer (OEC) development has been suggested, although spontaneous mutations of the BRCA1 gene in this disease are uncommon. Loss of gene function by epigenetic alteration is observed more commonly, while other means of gene inactivation have not been intensively investigated. We examined expression and localization of the BRCA1 gene product by immunohistochemistry and sought to clarify the relationship between protein expression and tumor stage, grade, histopathologic subtype, and outcome. Among 230 spontaneous OEC tumors, we found a statistically significant decrease in BRCA1 protein expression with advancing stages of OEC. There was no relationship between expression and tumor grade. There was a statistically significant relationship between the pathologic subtypes of OEC and BRCA1 expression. Minimal BRCA1 expression was protective for survival. These findings confirm a high rate of loss of BRCA1 protein expression in sporadic OEC and suggest a role of BRCA1 in the progression of sporadic ovarian carcinoma.
Comparison of equilibrium and non-equilibrium distribution coefficients for the human drug carbamazepine in soil

The distribution coefficient (KD) for the human drug carbamazepine was measured using a non-equilibrium technique. Repacked soil columns were prepared using an Airport silt loam (Typic Natrustalf) with an average organic matter content of 2.45%. Carbamazepine solutions were then leached through the columns at 0.5, 1.0 and 1.5 mL min(-1) representing average linear velocities of 1.8, 3.5 and 5.3 cm h(-1) respectively. Each flow rate was replicated three times and three carbamazepine pulses were applied to each column resulting in a total of 9 columns with 27 total carbamazepine pulses. Breakthrough curves were used to determine KD using the parameter fitting software CXTFIT. Results indicate that as flow rate decreased from 5.3 to 1.8 cm h(-1), KD increased an average of 21%. Additionally, KD determined by column leaching (14.7-22.7 L kg(-1)) was greater than KD determined by a 2h batch equilibrium adsorption (12.6 L kg(-1)). Based on these KD's carbamazepine would be generally characterized as non-mobile in the soil investigated. However, repeated carbamazepine applications resulted in an average 22% decrease in KD between the first and third applications. Decreasing KD is attributed to differences in sorption site kinetics and carbamazepine residence time in contact with the soil. This would indicate that the repeated use of reclaimed wastewater at high application rates for long-term irrigation or groundwater recharge has the potential to lead to greater transport of carbamazepine than KD determined by batch equilibrium would predict.
The management of anticoagulation during noncardiac operations in patients with prosthetic heart valves

Based on previous thromboembolic complications associated with the interruption of anticoagulation during subsequent noncardiac operations in patients with nonbiological mitral prostheses, a protocol was developed for this high risk group. We report the successful management of 26 such operations in which anticoagulation was interrupted for 12 hours and then rapidly restored by means of heparin in the postoperative period. Since an earlier study suggested no adverse effect from the interruption of chronic anticoagulants for three to five days among patients with isolated aortic valve prostheses, simple interruption was again employed during 16 subsequent noncardiac operative procedures in this group with no complications. There were three episodes of hemorrhage observed in patients receiving therapeutic doses of heparin postoperatively, but only one required blood replacement.
Bone Mineral Density, Carotid Artery Intimal Medial Thickness, and the Vitamin D Receptor BsmI Polymorphism in Mexican American Women

Low bone mineral density (BMD) is a predictor of cardiovascular mortality, suggesting that osteoporosis and cardiovascular disease may share common risk factors. We assessed the relationship between BMD and intimal medial thickening (IMT) of the common carotid artery, a marker of sub-clinical atherosclerosis, in 471 women examined as part of the San Antonio Family Osteoporosis Study, a population-based study of osteoporosis risk conducted in Mexican American families. Because of the documented role of vitamin D metabolism in bone metabolism and its possible role in cardiovascular function, we further evaluated whether allelic variation at the vitamin D receptor locus (VDR) influenced joint variation in BMD and IMT. The association of BMD with IMT depended on age, with low BMD being correlated with high IMT in older women, but with low IMT in younger women [age by IMT interaction effects significant at the spine (P = 0.042), radius ultradistal (P = 0.010), and hip (P = 0.006)]. In all women, the VDR BsmI BB genotype was associated with significantly higher forearm BMD (P = 0.005 for both radius ultradistal and midpoint), higher IMT (P = 0.05), and higher spine BMD in older women (P = 0.06), but not with hip BMD. The association of the VDR genotype with IMT was independent of its association with BMD. Although a functional consequence of the BsmI polymorphism on vitamin D metabolism has not been established, these findings support a possible biological relationship among VDR, bone metabolism, and atherosclerosis. We conclude that VDR polymorphisms may be one of multiple factors influencing the joint risk of atherosclerosis and osteoporosis.
The primary structure of rat ribosomal protein L12.

Summary The covalent structure of the rat 60S subunit protein L12 which is a component of the ribosomal elongation factor binding domain was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2-terminal amino acid sequence of the protein. L12 has 165 amino acids and a molecular weight of 17, 834. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 11–13 copies of the L12 gene. The mRNA for the protein is about 800 nucleotides in length. Rat L12 is homologous to Saccharomyces cerevisiae L15. The cDNA contains the highly repetitive DNA sequence, R. dre. I, in the 3′ noncoding region.
Absolute dating of igneous rocks of Crimea as reference formations for Bajocian

Because of the fairly homogeneous petrographic and chemical character of the assemblages of the Crimean igneous rocks (plutons), they can be used for an accurate geochronological correlation of the Middle Jurassic. Potassium-argon ages have established the ages of many of the Crimean intrusives as averaging 159 ± 3 m.y. Hypabyssal and effusive rocks (the Bajocian formations) genetically associated with the Crimean intrusives can therefore also be dated as 159 ± 3 m.y. Limits of the Triassic Period should be left at 135 and 180 (perhaps 185) m.y. Younger age determinations of 106 ± 5 m. y. suggest a regional rejuvenation of some of the Crimean intrusives in connection with low temperature hydrothermal metamorphism. Additional work should be carried out to further elucidate this fact. — R.M. Hutchinson.
2,5-Hexanedione exposure alters microtubule motor distribution in adult rat testis

2,5-Hexanedione (2,5-HD) exposure in rats causes a progressive Sertoli cell injury culminating in testicular atrophy. Morphological injury is preceded by alterations in the assembly characteristics of tubulin isolated from exposed rat testes. This is followed by decreased seminiferous tubule fluid (STF) secretion by Sertoli cells and an increase in the number and size of Sertoli cell vacuoles. The possible involvement of microtubules and microtubule motor-dependent transport processes in STF secretion by Sertoli cells prompted us to examine the immunodistribution of the microtubule motors cytoplasmic dynein and kinesin during and after 2,5-HD exposure in rats. Three weeks following the commencement of exposure (1% 2,5-HD in the drinking water), the intensity of apical Sertoli cell cytoplasmic dynein immunofluorescence declined. This staining deficit became statistically significant by 4 weeks of exposure. Accompanying this change, there was progressive disruption of the immunodistribution of cisternal Golgi elements and associated kinesin immunoreactivity. The decrease in apical Sertoli cell cytoplasmic dynein immunofluorescence and disruption of Golgi and kinesin immunoreactivity suggest that 2,5-HD-induced alterations in Sertoli cell-mediated transport and secretory events could involve deficits in microtubule-dependent motor function.
Direct tunneling leakage current and scalability of alternative gate dielectrics

We explore the scaling limits of alternative gate dielectrics based on their direct tunneling characteristics and gate leakage requirements for future complementary metal–oxide–semiconductor technology generations. Important material parameters such as the tunneling effective mass are extracted for several promising high-κ gate dielectrics. We also introduce a figure of merit for comparing the relative advantages of gate dielectric candidates. Using an accurate direct tunneling gate current model and specifications from the International Technology Roadmap for Semiconductors, we provide guidelines for the selection of gate dielectrics to satisfy the off-state leakage current requirements of future high-performance and low power technologies.
Factors affecting harp seal (Pagophilus groenlandicus) strandings in the Northwest Atlantic.

The effects of climate change on high latitude regions are becoming increasingly evident, particularly in the rapid decline of sea ice cover in the Arctic. Many high latitude species dependent on sea ice are being forced to adapt to changing habitats. Harp seals (Pagophilus groenlandicus) are an indicator species for changing high-latitude ecosystems. This study analyzed multiple factors including ice cover, demographics, and genetic diversity, which could affect harp seal stranding rates along the eastern coast of the United States. Ice cover assessments were conducted for the month of February in the Gulf of St. Lawrence whelping region from 1991–2010 using remote sensing data, and harp seal stranding data were collected over the same time period. Genetic diversity, which may affect how quickly species can adapt to changing climates, was assessed using ten microsatellite markers to determine mean d2 in a subset of stranded and by-caught (presumably healthy) seals sampled along the northeast U.S. coast. Our study found a strong negative correlation (R2 = 0.49) between ice cover in the Gulf of St. Lawrence and yearling harp seal strandings, but found no relationship between sea ice conditions and adult strandings. Our analysis revealed that male seals stranded more frequently than females during the study period and that this relationship was strongest during light ice years. In contrast, we found no significant difference in mean d2 between stranded and by-caught harp seals. The results demonstrate that sea ice cover and demographic factors have a greater influence on harp seal stranding rates than genetic diversity, with only a little of the variance in mean d2 among stranded seals explained by ice cover. Any changes in these factors could have major implications for harp seals, and these findings should be considered in the development of future management plans for the Arctic that incorporate climate variability.
Selective Autolysis of Nuclei as a Source of DNA Precursors in Paramecium aurelia Exconjugants

SYNOPSIS The fate of labeled DNA in macronuclear fragments of starving Paramecium aurelia exconjugants was studied by quantitative autoradiography. Labeled material originally contained in DNA of macronuclear fragments is incorporated into macronuclear anlagen. During the starvation period the mean number of macronuclear fragments per cell decreased exponentially while there was an approximately exponential increase in the volume of macronuclear anlagen. Fragments appeared to be selectively and individually autolyzed. Labeled material originally contained in fragments was largely if not completely conserved through 108 hr of starvation during which more than 90% of the fragments were lost. Soluble labeled material was detectable after autolysis of fragments began, but finally almost all labeled material was incorporated into macronuclear anlagen.
ERp29 regulates epithelial sodium channel functional expression by promoting channel cleavage

The epithelial Na+ channel (ENaC) plays a key role in the regulation of blood pressure and airway surface liquid volume. ERp29 is a 29-kDa thioredoxin-homologous endoplasmic reticulum (ER) protein ...
Renin–angiotensin system in ureteric bud branching morphogenesis: implications for kidney disease

Failure of normal branching morphogenesis of the ureteric bud (UB), a key ontogenic process that controls organogenesis of the metanephric kidney, leads to congenital anomalies of the kidney and urinary tract (CAKUT), the leading cause of end-stage kidney disease in children. Recent studies have revealed a central role of the renin–angiotensin system (RAS), the cardinal regulator of blood pressure and fluid/electrolyte homeostasis, in the control of normal kidney development. Mice or humans with mutations in the RAS genes exhibit a spectrum of CAKUT which includes renal medullary hypoplasia, hydronephrosis, renal hypodysplasia, duplicated renal collecting system and renal tubular dysgenesis. Emerging evidence indicates that severe hypoplasia of the inner medulla and papilla observed in angiotensinogen (Agt)- or angiotensin (Ang) II AT1receptor (AT1R)-deficient mice is due to aberrant UB branching morphogenesis resulting from disrupted RAS signaling. Lack of the prorenin receptor (PRR) in the UB in mice causes reduced UB branching, resulting in decreased nephron endowment, marked kidney hypoplasia, urinary concentrating and acidification defects. This review provides a mechanistic rational supporting the hypothesis that aberrant signaling of the intrarenal RAS during distinct stages of metanephric kidney development contributes to the pathogenesis of the broad phenotypic spectrum of CAKUT. As aberrant RAS signaling impairs normal renal development, these findings advocate caution for the use of RAS inhibitors in early infancy and further underscore a need to avoid their use during pregnancy and to identify the types of molecular processes that can be targeted for clinical intervention.
Report of the International Neutrino Commission: 2010

Abstract The International Neutrino Commission met during the conference to review NEUTRINO 2010, to discuss the format of the conference series, and to consider proposals for future conferences.
Population and target considerations for triple-negative breast cancer clinical trials

Triple-negative breast cancer (TNBC) is an aggressive disease subtype that has a poor prognosis. Extensive epidemiological evidence demonstrates clear socioeconomic and demographic associations with increased likelihood of TNBC in both poorer and minority populations. Thus, biological aggressiveness with few known therapeutic directions generates disparities in breast cancer outcomes for vulnerable populations. Emerging molecular evidence of potential targets in triple-negative subpopulations offers great potential for future clinical trial directions. However, trials must appropriately consider populations at risk for aggressive subtypes of disease in order to address this disparity most completely. New US FDA draft guidance documents provide both flexible outcomes for accelerated approvals as well as flexibility in design with adaptive trials. Careful planning with design, potential patient population and choices of molecular targets informed by biomarkers will be critical to address TNBC clinical care.
Fine Needle Aspiration Biopsy of Ocular Malignant Melanoma: An Institutional Review

Introduction: Ocular malignant melanoma (MM) is the most common primary intraocular cancer in adults. Ocular fine needle aspiration (FNA) biopsy is a high-risk procedure that may be performed during radioactive plaque insertion. Successful ocular FNA biopsies can provide information impacting prognosis and treatment. We review our experience with ocular FNA to examine its diagnostic efficacy and clinical significance. Materials and Methods: A search of the pathology database for ocular FNAs was performed between 1998 and 2012. Data was collected from reports including patient sex, age, race, and diagnosis. Chart review determined treatment and metastases. Results: 454 pathology reports of ocular FNA biopsies from 441 patients were reported (F Z 225; M Z 216; RaceZ 425 Caucasian; Age RangeZ 7 mo to 93 yr). The following diagnoses were rendered: MM (60.4%, 274/ 454), suspicious for MM (5.3%, 24/454), alternative diagnosis (34.4%, 156/ 454) (Table 1). Among 274 MM cases, 24.8% (68/274) were epithelioid, 23.0% (63/274) were spindle cell, 44.9% (123/274) were mixed cell subtypes, and 7.3% (20/274) were unspecified (Table 2). Among 156 cases with alternative diagnoses, 38.5% (60/156) were non-diagnostic, 12.2% (19/156) were atypical, 33.3% (52/156) were benign, 14.7% (23/156) were positive for non-melanoma malignancy, and 1.3% (2/156) were suspicious for malignancy (Table 1). Patients with a diagnosis of MM were commonly treated with a radioactive plaque (92.0%, 252/274) (Table 3). 12.8% (35/274) of patients with FNA biopsy-proven MM developed metastases, frequently identified in the liver (91.4%, 32/35), lung (28.6%, 10/35), and bone (25.7%, 9/35) (Table 4). Conclusions: Ocular FNA biopsies at our institution were performed during radioactive plaque insertion and were diagnostic for MM. Among nonmelanoma cases, most were either atypical or benign. A minority of patients developed metastases, with the most common site being the liver.
Biomarkers of brain injury in neonatal encephalopathy treated with hypothermia.

Objective To determine if early serum S100B and neuron-specific enolase (NSE)levels are associated with neuroradiographic and clinical evidence of brain injury in newborns with encephalopathy. Study design Patients who received therapeutic whole-body hypothermia were prospectively enrolled in this observational study. Serum specimens were collected at 0, 12, 24, and 72 hours of cooling. S100B and NSE levels were measured by enzyme linked immunosorbent assay. Magnetic resonance imaging was performed in surviving infants at 7-10 days of life. Standardized neurologic examination was performed by a child neurologist at 14 days of life. Multiple linear regression analyses were performed to evaluate the association between S100B and NSE levels and unfavorable outcome (death or severe magnetic resonance imaging injury/significant neurologic deficit). Cutoff values were determined by receiver operating curve analysis. Results Newborns with moderate to severe encephalopathy were enrolled (n = 75). Median pH at presentation was 6.9 (range, 6.5-7.35), and median Apgar scores of 1 at 1 minute, 3 at 5 minutes, and 5 at 10 minutes. NSE and S100B levels were higher in patients with unfavorable outcomes across all time points. These results remained statistically significant after controlling for covariables, including encephalopathy grade at presentation, Apgar score at 5 minutes of life, initial pH, and clinical seizures. Conclusion Elevated serum S100B and NSE levels measured during hypothermia were associated with neuroradiographic and clinical evidence of brain injury in encephalopathic newborns. These brain-specific proteins may be useful immediate biomarkers of cerebral injury severity. (J Pediatr 2012;161:434-40).
Chemical and biological consequences of oxidatively damaged guanine in DNA.

AbstractOf the four native nucleosides, 2′-deoxyguanosine (dGuo) is most easily oxidized. Two lesions derived from dGuo are 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (Fapy)∙dGuo. Furthermore, while steady-state levels of 8-oxodGuo can be detected in genomic DNA, it is also known that 8-oxodGuo is more easily oxidized than dGuo. Thus, 8-oxodGuo is susceptible to further oxidation to form several hyperoxidized dGuo products. This review addresses the structural impact, the mutagenic and genotoxic potential, and biological implications of oxidatively damaged DNA, in particular 8-oxodGuo, Fapy∙dGuo, and the hyperoxidized dGuo products.
Hydraulic permeation of liquids through swollen polymeric networks. III. A generalized correlation

An approximate equation was developed that permits calculation of the solvent tracer diffusion coefficient in a homogeneous swollen membrane from the measured hydraulic permeability coefficient. This relation was applied to data for 28 polymer–solvent systems that included 15 different organic solvents and 5 hydrocarbon polymer networks whose equilibrium swellings ranged from 16.1 to 91.5% polymer on a volume basis. The calculated tracer diffusion coefficient divided by the pure solvent self-diffusion coefficient for these systems formed a unique correlation when plotted versus the polymer volume fraction in the membrane. This relation agreed well with tracer diffusion coefficient data in the literature for the benzene–natural rubber system measured by radioactive tagging. Discussion centers on the evidence for the validity of the equation developed and the role of hydrodynamics on diffusion in swollen membranes.
Given over to demand: excorporation as commitment

This commentary discusses a set of essays, arguing that each examines a demand for the extraction or ‘excorporation’ of organic form to supplement a lack elsewhere, whether the apperception of that lack be located in the clinic, in national or regional politics, or in big data as databases come to promise the ground of inclusion and future life. Reading through and across the essays, it proposes a conceptual vocabulary rooted in a figure of ‘commitment’ or the ‘given over’, this given over opposed to an analytics of the gift. The argument is made that the body emerges as a ground for thought and action in its being given over to another, in its commitment. An analytics of commitment addresses the relation of parts and wholes in our attending to practices of excorporation and designations of sacrifice.
How badly do we need theory Z

In Theory Z-style management everybody participates in corporate decision making. This more open process should give us fewer Pintos, Love Canals, and massive international payoffs as executives are forced to expose their reasoning to the moral sensibilities of the whole corporation. So far everything looks good. But we are a long way from showing that only corporations so managed can be fully moral. Yet Dwiggins seems to believe this, putting his faith in the basic goodness of the many while virtually dismissing the managers as mere technicians. This is too slick; even if it describes our average corporation today, there are plenty of less radical changes which can also produce the desired moral corporation. Good leadership which acts on their commitment to improve the company is surely one historically respectable alternative; cynicism about the present crop of leaders should not distract us from investigating this route. Finally, I wonder if Dwiggins can consistently urge us to embrace a full-scale Theory Z organizational structure, given his position on the place of ethics and profitability.
An Open-Ended Project: Building a High Performance, yet Simple, Household Battery

An open-ended activity for high school students and college students is described. Students constructed a battery (galvanic cell) from common household materials such as aluminum, copper, table salt, and Drano. The battery yielded voltages ranging from 1.2 to 1.9 V, which are higher than those from a typical Zn�Cu lemon cell or a recently reported aluminum�air battery, and a current density of 33 mA/cm2 that is up to 75 times better than that of the aluminum�air battery and 150 times better than those of the typical lemon batteries. Students carried out various tests on the battery to gain an understanding of the factors affecting the cell performance, such as effect of household materials, NaCl in the electrolyte solution, electrode surface area, and electrode distance. Then, students, working in groups, were tasked to build a high-performance battery using common household materials. The final battery assembly should be able to power the motors of various toy cars. The project allowed students to relate chemistry to their everyday lives, gain additional insight in the concepts and principles of electrochemistry, and receive valuable hands-on experiences with electronics, circuits, and battery technology.
The uptake of cobalt into ferromanganese nodules, soils, and synthetic manganese (IV) oxides

Strong enrichments of cobalt occur in marine manganese nodules, soils, wads, and natural and synthetic minerals such as hollandite, cryptomelane, psilomelane, lithiophorite, birnessite, and δ-MnO2. Previously, it was suggested that Co3+ ions in these minerals replace either Mn3+ or substitute for Fe3+ in incipient goethite epitaxially intergrown with δ-MnO2. Neither of these interpretations is now considered to be satisfactory on account of the large discrepancy of ionic radius between octahedrally coordinated low-spin Co3+ and high-spin Mn3+ or Fe3+ in oxide structures. The close agreement between the ionic radii of Co3+ and Mn4+ suggests that some cobalt substitutes for Mn4+ ions in edge-shared [MnO6] octahedra in many manganese(IV) oxide mineral structures. It is proposed that hydrated cations, including Co2+ ions, are initially adsorbed on to the surfaces of certain Mn(IV) oxides in the vicinity of essential vacancies found in the chains or sheets of edge-shared [MnO6] octahedra. Subsequently, fixation of cobalt takes place as a result of oxidation of adsorbed Co2+ ions by Mn4+ and replacement of the displaced manganese by low-spin Co3+ ions in the [MnO6] octahedra or vacancies.
Labor markets and sunday closing laws

This paper puts forth arguments regarding the occurrence of state Sunday closing laws and reports statistical estimates that predict the occurrence of those laws in 1970 and 1984. Along with other socio-economic variables, two labor market activities are found to be significant determinants of Blue laws. Membership in labor unions is found to have a positive declining influence on the laws; female labor market participation is found to have a negative but growing influence.
The Place of Foreign Languages in a Unified Liberal Arts Program

Author's Summary— The place of foreign languages in a unified liberal arts program is determined by their relationship to a unifying principle: liberation of the mind from: (1) the bonds of ignorance; (2) the bewildering complexity of human experience; (3) the limitations of space and time; (4) the tyranny of symbols; (5) the bonds of self.
Surface Energy Characterization of Three Partially Hydrolyzed Hardwood Species Determined by Dynamic Contact Angle Analysis

The effects of a partial hydrolysis treatment on the surface energies of three southern hardwood species, red oak, sweetgum, and yellow-poplar were examined. Miniature beams machined to 3 × 15 × 150 mm (t × r × l) were water-saturated and subjected to a partial hydrolysis at 150°C for 30 min in 1% sulfuric acid, water, or 1% sodium hydroxide solutions. Untreated wood beams were used as controls. Dynamic contact angle specimens were planed from the beams to a thickness of 0.25 mm and cut to a length of 15 mm for analysis via the Wilhelmy plate technique. Surface energies for the wood/treatment combinations were calculated using the geometric mean procedure. Results indicated that acid and water treatments improved the wettability for all species. Alkaline treatment effects were species-specific. All treatments improved the surface energy of red oak. The dispersive forces accounted for the majority of the surface energies.
Differential effects of electroconvulsive shock and antidepressant drugs on serotonin-2 receptors in rat brain.

The effects of chronic administration of electroconvulsive shock and antidepressant drugs on rat brain serotonin-2 (5-HT2) receptors were investigated. Electroconbulsive shock increases the density and antidepressant drugs decrease the density of 5-HT2 receptors labelled by [3H]spiperone.
Effect of neonatal thymectomy and antilymphocyte globulin on non-specific mitogen stimulation in Holstein-Friesian calves

Nine of seventeen neonatal Holstein-Friesian calves were thymectomized, treated with antilymphocyte globulin, and monitored for immunologic functional ability for 4 to 6 months. The thymus weights for 4 to 10-day-old calves and 4 to 6-month-old calves indicated a continued increase in total weight. This indicated significant thymic involution had not occurred at 4 to 6 months. Following thymectomy a wasting syndrome was not observed although an increased incidence of a lowly virulent virus infection did occur. A significant decrease in circulating lymphocytes was observed. Peripheral blood lymphocytes were stimulated in vitro by non-specific mitogens, phytohemagglutinin, bacterial lipopolysaccharide and pokeweed mitogen using the whole blood culture method. Observations included a greater response to phytohemagglutinin and pokeweed mitogen in summer months and variable age related response to all mitogens. Lipopolysaccharide stimulation results were inconclusive. It was concluded that neonatal thymectomy was not a satisfactory experimental procedure for the production of selective immunosuppression in the bovine species.
Ultrafine-Grained Al-Mg-Sc Alloy via Friction-Stir Processing

Friction-stir processing (FSP) of twin-roll cast (TRC) Al-Mg-Sc alloy resulted into ultrafine-grained microstructure. The alloy was processed in as-received and aged (563 K [290 °C], 22 hours) conditions and at three different tool rotation rates: 800, 400, and 325 rpm. The microstructural features were characterized using electron backscattered diffraction (EBSD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The grain size varied from 0.89 μm to 0.39 μm depending on the processing and initial thermo-mechanical conditions of the alloy. The TRC alloy processed at 325 rpm in aged condition had all the grains less than 1 μm, and 95 pct of grains had high-angle grain boundaries (HAGBs). In all the cases, the fraction of HAGBs were more than 80 pct. The variation of misorientation angle distribution was similar to the theoretical MacKenzie distribution for cubic crystal materials. Grain size analysis at different sections and locations on the transverse section of the dynamically recrystallized zone showed a homogeneous and equiaxed microstructure. The average dispersoid (Al3(Sc,Zr)) size was ~8.0 nm in diameter obtained using high-resolution TEM. Grain size reduction was observed with increase in Zener–Hollomon parameter. It was shown that under the current microstructural and deformation conditions, dynamic recrystallization via particle-stimulated nucleation might not be possible during FSP.
Life adjustment of retired couples.

The marital relationship and retirement experiences of a community resident sample of retired couples from working class backgrounds has been investigated. Data was obtained from a structured clini...
Vertical skeletal and dental changes in earlytreatment of class II malocclusion

Increases in the vertical dimension can be detrimental in certain facial types or unstable in others. By using cephalograms, this study examined the skeletal and dental changes as well as their permanence in a group of children participating in a randomized clinical trial on the timing of Class II orthodontic treatment. Children (mean age, 9.6 years) were assigned to either a headgear/biteplane (n = 94), bionator (n = 86), or observation (n = 82) group. Cephalometric data were obtained from tracings at baseline, after Class I molar was obtained or 2 years after treatment had elapsed, and after 1 year of retention or observation. In general, the skeletal changes, such as total anterior and posterior face height and mandibular plane angle, observed during the active phase of early treatment persisted until the end of phase I. Phase I treatment also produced dental changes, such as molar eruption and tip. With the exception of the eruption of the mandibular molars in the bionator group, these changes could not be maintained until the end of phase I. Whether the subjects were in retention or not had little impact on any of the studied parameters. Mandibular plane angle, pretreatment, and retention had an affect on the mandibular molar tip over the course of the study.
Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

Plasma membrane proteins are a large, diverse group of proteins comprised of receptors, ion channels, transporters and pumps. Activity of these proteins is responsible for a variety of key cellular events, including nutrient delivery, cellular excitability, and chemical signaling. Many plasma membrane proteins are dynamically regulated by endocytic trafficking, which modulates protein function by altering protein surface expression. The mechanisms that facilitate protein endocytosis are complex and are not fully understood for many membrane proteins. In order to fully understand the mechanisms that control the endocytic trafficking of a given protein, it is critical that the protein s endocytic rate be precisely measured. For many receptors, direct endocytic rate measurements are frequently achieved utilizing labeled receptor ligands. However, for many classes of membrane proteins, such as transporters, pumps and ion channels, there is no convenient ligand that can be used to measure the endocytic rate. In the present report, we describe a reversible biotinylation method that we employ to measure the dopamine transporter (DAT) endocytic rate. This method provides a straightforward approach to measuring internalization rates, and can be easily employed for trafficking studies of most membrane proteins.
Evolution and Conservation of Predicted Inclusion Membrane Proteins in Chlamydiae

Chlamydia spp. are obligate intracellular pathogens that replicate within a vacuole termed the inclusion. Chlamydiae extensively modify the inclusion membrane via the insertion of chlamydial inclusion membrane proteins (Incs) which decorate the cytosolic face of the inclusion. We have assessed the overall relatedness and phylogeny of Incs in order to identify potential evolutionary trends. Despite a high degree of conservation among Incs within C. trachomatis serovars, phylogenetic analysis showed that some Incs cluster according to clinical groupings suggesting that certain Incs may contribute to tissue tropism. Bioinformatic predictions identified Incs in five chlamydial species: 55 in C. trachomatis, 68 in C. felis, 92 in C. pneumoniae, 79 in C. caviae, and 54 in C. muridarum. Inc homologues were compared between chlamydial species and 23 core Incs were identified as shared among all species. Genomic expansion of Incs was identified in C. pneumoniae, C. caviae, and C. felis but not C. trachomatis or C. muridarum.
Critical Review of the Test of Gross Motor Development

The Test of Gross Motor Development (TGMD) is designed to be both a norm-referenced and criterion-referenced assessment of gross motor skills in 3-to 10- year-old children, normal to moderately handicapped. Whether the TGMD thoroughly covers the total domain of gross motor function with its two subtests of locomotor and object control is questioned. The TGMD appears promising as a test the is easily learned and administered by a wide variety of professionals. Studies are reviewed that address the issues if the TGMD's reliability and validity. As with any formal assessment, testers need to be aware of the TGMD's strengths and weaknesses in interpreting test scores.
Platelets, glycoprotein Ib-IX, and von Willebrand factor are required for FeCl3-induced occlusive thrombus formation in the inferior vena cava of mice

Venous thromboembolism is a leading cause of death from cardiovascular disease. Despite the importance of the glycoprotein (GP) Ib-IX/von Willebrand factor (vWF) axis in arterial thrombosis, its requirement in venous, not venule thrombosis in response to endothelial injury (not stenosis or stasis) is uncharacterized. GPIbα-vWF participation in FeCl3-induced thrombus formation was evaluated in the inferior vena cava (IVC). Stable, occlusive thrombus formation in response to FeCl3-induced injury of the IVC was studied. FeCl3 (20% FeCl3, 10 minutes)-induced occlusive thrombosis required platelets as confirmed by a lack of occlusion in thrombocytopenic mice, and stable occlusion in control animals. No IVC occlusion was observed using GPIbα-deficient animals, a model of the human Bernard-Soulier syndrome (BSS). Transgenic IL-4 R/GPIbα mice (lack murine GPIbα, but express the extracellular domain of the human interleukin (IL-4 receptor fused to the transmembrane and cytoplasmic domains of human GPIbα) were stud...
Phylogenetic composition of Arctic Ocean archaeal assemblages and comparison with Antarctic assemblages.

Archaea assemblages from the Arctic Ocean and Antarctic waters were compared by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes amplified using the Archaea-specific primers 344f and 517r. Inspection of the DGGE fingerprints of 33 samples from the Arctic Ocean (from SCICEX submarine cruises in 1995, 1996, and 1997) and 7 Antarctic samples from Gerlache Strait and Dallman Bay revealed that the richness of Archaea assemblages was greater in samples from deep water than in those from the upper water column in both polar oceans. DGGE banding patterns suggested that most of the Archaea ribotypes were common to both the Arctic Ocean and the Antarctic Ocean. However, some of the Euryarchaeota ribotypes were unique to each system. Cluster analysis of DGGE fingerprints revealed no seasonal variation but supported depth-related differences in the composition of the Arctic Ocean Archaea assemblage. The phylogenetic composition of the Archaea assemblage was determined by cloning and then sequencing amplicons obtained from the Archaea-specific primers 21f and 958r. Sequences of 198 clones from nine samples covering three seasons and all depths grouped with marine group I Crenarchaeota (111 clones), marine group II Euryarchaeota (86 clones), and group IV Euryarchaeota (1 clone). A sequence obtained only from a DGGE band was similar to those of the marine group III Euryarchaeota.
Markov jump-linear performance models for recoverable flight control computers

Single event upsets in digital flight control hardware induced by atmospheric neutrons can reduce system performance and possibly introduce a safety hazard. One method currently under investigation to help mitigate the effects of these upsets is NASA Langley's recoverable computer system. In this paper, a Markov jump-linear model is developed for a recoverable flight control system, which will be validated using data from future experiments with simulated and real neutron environments. The method of tracking error analysis and the plan for the experiments are also described.
Identification of Multiple Rate-limiting Steps during the Human Mitochondrial Transcription Cycle in Vitro

We have reconstituted human mitochondrial transcription in vitro on DNA oligonucleotide templates representing the light strand and heavy strand-1 promoters using protein components (RNA polymerase and transcription factors A and B2) isolated from Escherichia coli. We show that 1 eq of each transcription factor and polymerase relative to the promoter is required to assemble a functional initiation complex. The light strand promoter is at least 2-fold more efficient than the heavy strand-1 promoter, but this difference cannot be explained solely by the differences in the interaction of the transcription machinery with the different promoters. In both cases, the rate-limiting step for production of the first phosphodiester bond is open complex formation. Open complex formation requires both transcription factors; however, steps immediately thereafter only require transcription factor B2. The concentration of nucleotide required for production of the first dinucleotide product is substantially higher than that required for subsequent cycles of nucleotide addition. In vitro, promoter-specific differences in post-initiation control of transcription exist, as well as a second rate-limiting step that controls conversion of the transcription initiation complex into a transcription elongation complex. Rate-limiting steps of the biochemical pathways are often those that are targeted for regulation. Like the more complex multisubunit transcription systems, multiple steps may exist for control of transcription in human mitochondria. The tools and mechanistic framework presented here will facilitate not only the discovery of mechanisms regulating human mitochondrial transcription but also interrogation of the structure, function, and mechanism of the complexes that are regulated during human mitochondrial transcription.
Incidence of Plantar Fascia Ruptures Following Corticosteroid Injection

Plantar fasciitis is commonly treated with corticosteroid injections to decrease pain and inflammation. Therapeutic benefits often vary in terms of efficacy and duration. Rupture of the plantar fascia has been reported as a possible complication following corticosteroid injection. A retrospective chart review of 120 patients who received corticosteroid injection for plantar fasciitis was performed at the authors' institution to determine the incidence of plantar fascia rupture. The plantar fascia rupture was diagnosed clinically and confirmed with magnetic resonance imaging. Various factors were analyzed, including the number of injections, interval between injections, body mass index (BMI), and activity level. Four patients (2.4%) consequently experienced plantar fascia rupture following an average of 2.67 injections. The average BMI of these patients was 38.6 kg/m². The authors conclude that corticosteroid injection therapy appears to be a safe and effective form of nonoperative treatment with minimal complications and a relatively low incident of plantar fascia rupture.
Pigmented Soybean (Glycine max) Seed Coats Accumulate Proanthocyanidins during Development

The dominant I gene inhibits accumulation of anthocyanin pigments in the epidermal layer of soybean (Glycine max) seed coats. Seed-coat color is also influenced by the R locus and by the pubescence color alleles (T, tawny; t, gray). Protein and RNA from cultivars with black (i,R,T) and brown (i,r,T) seed coats are difficult to extract. To determine the nature of the interfering plant products, we examined seed-coat extracts from Clark isogenic lines for flavonoids, anthocyanins, and possible proanthocyanidins by thin-layer chromatography. We show that yellow seed-coat varieties (I) do not accumulate anthocyanins (anthocyanidin glycosides) or proanthocyanidins (polymeric anthocyanidins). Mature, black (i,R,T) and imperfect-black (i,R,t) seed coats contained anthocyanins, whereas mature, brown (i,r,T) and buff (i,r,t) seed coats did not contain anthocyanins. In contrast, all colored (i) genotypes tested positive for the presence of proanthocyanidins by butanol/ HCl and 0.5% vanillin assays. Immature, black (i,R,T) and brown (i,r,T) seed coats contained significant amounts of procyanidin, a 3[prime],4[prime]-hydroxylated proanthocyanidin. Immature, black (i,R,T) or brown (i,r,T) seed-coat extracts also tested positive for the ability to precipitate proteins in a radial diffusion assay and to bind RNA in vitro. Imperfect-black (i,R,t) or buff (i,r,t) seed coats contained lesser amounts of propelargonidin, a 4[prime]-hydroxylated proanthocyanidin. Seed-coat extracts from these genotypes did not have the ability to precipitate protein or bind to RNA. In summary, the dominant I gene controls inhibition of not only anthocyanins but also proanthocyanidins in soybean seed coats. In homozygous recessive i genotypes, the T-t gene pair determines the types of proanthocyanidins present, which is consistent with the hypothesis that the T locus encodes a microsomal 3[prime]-flavonoid hydroxylase.
What's Special about U.S. Muslims? The War on Terrorism as Seen by Muslims in the United States, Morocco, Egypt, Pakistan, and Indonesia

Since 11 September 2001, Europe has suffered multiple jihadi attacks but the United States has not. This “American exceptionalism” has been attributed to the special qualities of U.S. Muslims, who are seen as politically better integrated and less sympathetic toward radical politics than other Muslims. This article tests the exceptionalism hypothesis by comparing results from a 2007 Pew poll of U.S. Muslims with results from 2006–2007 START polls of Muslims in Morocco, Egypt, Pakistan, and Indonesia. On questions about religious identity, attitude toward Al Qaeda, U.S. intentions in the War on Terrorism, and suicide terrorism, U.S. Muslims differed only slightly from comparison Muslims.
A platform for predicting and enhancing model drug delivery across the human nail plate.

Purpose: The objective of the present study was to assess the effect of pretreatment using chemical etchants on the delivery of terbinafine hydrochloride (TH) and 5-fluorouracil (5-FU) into and across the human nail plate. Methods: The TranScreen-N method was used to screen five potential etchants. Based on these results, the dorsal surface of nails was pretreated with chemical etchants, 1% or 10% (w/w) phosphoric acid (PA) or 10% (w/w) lactic acid (LA) gels, for a period of 60 seconds. The nail pretreated with a plain gel formulation (no PA or LA incorporated) was used as the control. Results: Despite the differences in physicochemical properties between TH (log P = 3.3) and 5-FU (log P = −0.83), the in vitro permeation as well as drug load of these drugs in the nail plate was enhanced because of pretreatment with the PA gels, whereas LA pretreatment failed to enhance the drug load and permeation. Optical microscopic and atomic force microscopy studies revealed that the PA enhanced the trans-ungual drug ...