cssi/SciSciGPT-SciSciNet huggingface dataset:
Cystic cerebellar dysplasia and biallelic LAMA1 mutations: a lamininopathy associated with tics, obsessive compulsive traits and myopia due to cell adhesion and migration defects

Background Laminins are heterotrimeric complexes, consisting of α, β and γ subunits that form a major component of basement membranes and extracellular matrix. Laminin complexes have different, but often overlapping, distributions and functions. Methods Under our clinical protocol, NCT00068224, we have performed extensive clinical and neuropsychiatric phenotyping, neuroimaging and molecular analysis in patients with laminin α1 ( LAMA1 )-associated lamininopathy. We investigated the consequence of mutations in LAMA1 using patient-derived fibroblasts and neuronal cells derived from neuronal stem cells. Results In this paper we describe individuals with biallelic mutations in LAMA1 , all of whom had the cerebellar dysplasia, myopia and retinal dystrophy, in addition to obsessive compulsive traits, tics and anxiety. Patient-derived fibroblasts have impaired adhesion, reduced migration, abnormal morphology and increased apoptosis due to impaired activation of Cdc42, a member of the Rho family of GTPases that is involved in cytoskeletal dynamics. LAMA1 knockdown in human neuronal cells also showed abnormal morphology and filopodia formation, supporting the importance of LAMA1 in neuronal migration, and marking these cells potentially useful tools for disease modelling and therapeutic target discovery. Conclusion This paper broadens the phenotypes associated with LAMA1 mutations. We demonstrate that LAMA1 deficiency can lead to alteration in cytoskeletal dynamics, which may invariably lead to alteration in dendrite growth and axonal formation. Estimation of disease prevalence based on population studies in LAMA1 reveals a prevalence of 1–20 in 1 000 000. Trial registration number: NCT00068224
Effective Treatment of Experimental Glioblastoma by HSV Vector-Mediated TNFα and HSV-tk Gene Transfer in Combination with Radiosurgery and Ganciclovir Administration

Experiments were carried out in a nude mouse model of human glioblastoma to determine whether gamma-knife radiosurgery combined with herpes simplex virus thymidine kinase (tk) suicide gene therapy and tumor necrosis factor alpha (TNFalpha) gene transfer provided an improved multimodality treatment of this disease. Animals were inoculated intracerebrally with 2 x 10(5) U-87MG human glioblastoma cells to establish brain tumors. At 3 days postinoculation, the tumor region was injected with 2 x 10(6) infectious particles of highly defective herpes simplex viral vectors expressing the viral tk gene with the kinetics of a viral immediate early gene either alone (T.1) or together with TNF alpha (TH:TNF). Subgroups of animals were given daily intraperitoneal injections of ganciclovir (GCV) for 10 days and/or subjected to gamma-knife radiosurgery on the fifth day post tumor-cell implantation. Comparisons of animal survival showed that the TH:TNF vector in combination with radiosurgery and GCV administration provided the most effective therapy; eight of nine animals survived for 75 days compared to four of eight using the next best protocol. These findings suggest that gene therapy in combination with more conventional therapeutic methods may provide an improved strategy for extending the life expectancy of patients afflicted with this ultimately fatal disease.
In-Transit Shipboard Fumigation of Corn on a Tanker Vessel

Corn aboard a tanker vessel was fumigated in transit with aluminum phosphide tablets at the rate of 1.06 g/m3 and with Detia gas bag blankets at the rate of 1.17 g/m3 Gas concentrations were always highest on the surface of the grain; however, some gas was detected at all sample points during the first half of the 44-day fumigation. The ship’s stripper pump was used to facilitate rapid penetration of phosphine in one fumigated tank, but the gas channeled downward directly to the rose box and was not uniformly distributed. To assess safety to the crew, phosphine was monitored in transit three times daily at 26 selected locations throughout the living and working areas of the ship. After fumigation, one tank was found to have a cracked bulkhead, and phosphine levels exceeding the threshold limit value were observed in an adjacent forward locker room. These concentrations dissipated quickly and completely after the locker room door was opened. Although live insects were on-loaded in the com, none were found during unloading. Phosphine residues were lowest on corn fumigated with bag blankets; residues exceeding the tolerance of 100 ppb were found on samples taken from the surface of the com in tanks treated with tablets.
Modification of development in chick embryos induced by ultra-violet radiation.

This is the sixth of a series of studies on radiation, four of which have concerned themselves with differential modification of embryonic development. The previous work includes experiments on Arbacia eggs and sperm, and on Fundulus eggs. The results in all the experiments agree in their more general features, namely, those regions which at the time of exposure have the highest rates of physiological activity, are the most readily modified in their development. Such modifications may be brought about by exposures made at various intervals after fertilization, as well as before fertilization. Experiments with hens' eggs yield the same general results, when exposed before and after incubation.The complete spectrum of the Hg vapor arc (running at 110 v. D. C. at a distance of 10 inches) served as a source of radiation. Eggs were exposed at intervals, up to 64 hr. after incubation, as well as before incubation. Since it was found that exposures through the shell were entirely without effect, it was necessary...
Effect of digitoxin and quinidine on intracellular electrolytes of the rabbit heart.

Conclusions1. 32 rabbits were injected intramuscularly in the gluteal region with 0.12 g of quinidine gluconate twice daily and 21 rabbits were similarly injected daily with 0.3 mg digitaline nativelle for 5 days and 10 days respectively. 2. A gain in intracellular cardiac potassium as expressed in liters of intracellular water was observed in the quinidine treated animals (from 108 to 124 mEq). A loss in intracellular cardiac sodium from 13.6 to 6.8 was also observed. 3. The mechanism of digitoxin toxicity and its reversal by quinidine is discussed. It is suggested that one of the actions of quinidine may be to reverse the ionic flow of sodium and potassium (and perhaps other ions) in cardiac muscle which occurs in digitoxin toxicity.
A New In-Cage Treatment System for Control of Northern Fowl Mites on Laying Hens

Commercially available plastic and other types of flexible tubing were tested to determine their relative permeability to an emulsifiable concentrate (EC) of permethrin acaricide. Bioassays employing northern fowl mites, Ornithonyssus sylviarum (Canestrini and Fanzago), indicated that .3 mg permethrin/cm2 surface area penetrated vinyl plastic tubing that had been filled with 42.5% active ingredient EC for 14 days. Vinyl tubing circuits were installed in wire poultry cages, either in the form of suspended loops or woven through the rear cage wall, and permethrin EC was introduced by means of a small pump. Significant reductions in northern fowl mite populations on hens caged therein were apparent after 48 days posttreatment. This approach has merit as a long-term, labor-economical form of mite control on caged poultry.
The Otalgia Point: A Novel Clinical Gesture in Otolaryngology.

Introduction:In cases of otalgia without any accompanying findings, some patients locate their otalgia below the attachment of the lobule, at the apex of the jugulodigastric region.Purpose:To present a series of these patients for whom nasal steroids or myringotomy usually ameliorated their pain.Materials and Methods:Thirty-two patients with normal physical examinations, tympanograms, and age-appropriate audiograms spontaneously indicated otalgia at “the otalgia point.” Inspection of the oropharynx and nasal airway, palpation of the neck and temporal mandibular joints, and nasopharyngoscopy/laryngoscopy ruled out referred causes of otalgia. Patients were offered either nasal steroid spray or trial myringotomy followed by tympanostomy tube.Results:Otalgia improved in all 10 (100%) patients who selected nasal steroids. Otalgia resolved in 17 of 20 (85%) myringotomy participants. Three patients declined intervention. In all, symptoms improved in 27/29 treated patients (93%).Conclusion:This description of “th...
Avocado extracts selectively induce apoptosis in transformed human oral cells

A132 Diets rich in fruits and vegetables has been associated with reduced risks for many types of cancers. Avocado (Persea americana) is a widely consumed fruit containing many cancer preventing nutrients, vitamins and phytochemicals. Additionally, the avocado is sodium, cholesterol and sugar free, and low in calories and fats making it a part of a healthy diet. We have shown that avocado extract D003 selectively induced apoptosis in premalignant and malignant human oral epithelial cell lines via accumulation of reactive oxygen species (ROS). To further test the potential activity of avocado extract selectively targeting precancerous cells, primary human oral epithelial cells (TE1177) were transformed with retrovirus containing HVP16/E6 or HVP16/E7. Infection of either HVP16/E6 or E7 successfully immortalized primary human oral epithelial cells. Two cell line from E6 (TE1177/E6) and five cell lines from E7 (TE1177/E7) were established. Flow cytometry analysis found that all seven cell lines harbored higher basic levels of ROS compared to the parent TE1177 cells. ROS levels rose dramatically in these transformed cell lines upon the treatment of avocado extracts for 3 hrs. Growth inhibition assay found that the transformed cells were more sensitive to the treatment of avocado extract than TE1177 cells. The addition of N-Acetyl-Cysteine (NAC) markedly reduced the levels of the ROS produced by the treatment of cells with the D003 extract and partially reversed D003-induced growth inhibition in transformed cells. Our data demonstrates that avocado extracts have potent inhibitory effect on the proliferation and selective killing of human oral transformed/premalignant and malignant cells. This inhibition appears to be mediated via ROS induced apoptosis. The further identification and characterization of the active components in avocados and their molecular targets in premalignant and malignant cells should help to better establish the nutritional components responsible for food-based chemopreventive activity.
Fast multiplicity counter featuring stilbene detectors for special nuclear material assay

A fast neutron multiplicity counting system based on organic scintillators, i.e. EJ-309 and stilbene, has been developed and experimentally tested at the University of Michigan. The system is able to detect correlated photon and neutron multiplets emitted by the fission reaction, within a gate time of tens of nanoseconds. This counting strategy is exploited to quantify fissile mass, without the need of complex electronic circuitry and unfolding procedures, otherwise required in moderated systems. Moderated systems are traditionally based on helium-3 detectors and feature a gate time of hundreds of microseconds. Measurement precision is thus negatively affected by accidental coincidences. A prototypal version of the proposed well-shaped counter was assembled and tested in the laboratory, using a spontaneous fission and an (α, n) neutron source, i.e. 252Cf and PuBe respectively. Measured results show excellent agreement with the simulated model of the system. The viability of the system to discriminate time-correlated fission neutrons from random, uncorrelated neutrons was proved. Preliminary results of an experimental campaign, carried out at INL (Idaho National Laboratory), to characterize plutonium metal samples are also shown. Results show a monotonic increasing trend for the range of measured 240-Pu effective masses, i.e. 0.024–0.5 kg, enabling the measurement of the mass of an unknown sample.
Sacred Objects as Vital Objects: Transitional Objects Reconsidered:

In this essay I argue that the concept of transitional objects in Winnicott's psychoanalytic developmental theory and Rizzuto's perspective regarding God representations in human life, though helpf...
Surgical Technical Evidence Review for Elective Total Joint Replacement Conducted for the AHRQ Safety Program for Improving Surgical Care and Recovery.

Background:Use of enhanced recovery pathways (ERPs) can improve patient outcomes, yet national implementation of these pathways remains low. The Agency for Healthcare Research and Quality (AHRQ; fu...
1,3,5-Tris-(4-(iso-propyl)-phenylsulfamoylmethyl)benzene as a potential Am(III) extractant: experimental and theoretical study of Sm(III) complexation and extraction and theoretical correlation with Am(III)

The problem of legacy alkaline high-level waste (HLW) both in the US and Russia, as a result of weapons production, has prompted studies of ligands for extraction of actinides, that could be possib...
Conceptualizations of Context in Science Education Research: Implications for Equity

Context is commonly equated to the setting in which events take place. Researchers often capture this place in their descriptions of participants, physical surroundings, and artifacts relevant to the phenomenon under investigation. In this chapter, context is treated as part of the researched phenomena. The first treatment of context considers factors internal and external to the individual. This conceptualization of context is illustrated via research on middle school science teachers’ views about students in relation to their life experiences situated within the sociopolitical milieux of the societies in which they occurred. Fields, sites that are separated both temporally and spatially constitute the second conceptualization of context. Fields have resources that promote structure; agency within a field enables access to and appropriation of these resources. Social life within a field is mediated by a dialectical relationship between agency and structure and this second view of context examines this relationship by way of cogenerative dialogues (Roth et al., Learn Environ Res 5(1):1–28, 2002). Cogenerative dialogues (or cogens) are fields within which participants engage in critical reflection by making meaning of shared experiences through multiple understandings and opportunities.
Irradiated Nuclear Fuel Management: Resource Versus Waste

Management of irradiated fuel is an important component of commercial nuclear power production. Although it is broadly agreed that the disposition of some fraction of the fuel in geological repositories will be necessary, there is a range of options that can be considered that affect exactly what fraction of material will be disposed in that manner. Furthermore, until geological repositories are available to accept commercial irradiated fuel, these materials must be safely stored. Temporary storage of irradiated fuel has traditionally been conducted in storage pools, and this is still true for freshly discharged fuel. Criticality control technologies have led to greater efficiencies in packing of irradiated fuel into storage pools. With continued delays in establishing permanent repositories, utilities have begun to move some of the irradiated fuel inventory into dry storage. Fuel cycle options being considered worldwide include the once-through fuel cycle, limited recycle in which U and Pu are recycled back to power reactors as mixed oxide fuel, and advance partitioning and transmutation schemes designed to reduce the long term hazards associated with geological disposal from millions of years to a few hundred years. Each of these options introduces specific challenges in terms of the waste forms required tomore » safely immobilize the hazardous components of irradiated fuel.« less
Fundamentals of Accelerators for Single-Pass Free Electron Lasers

The characteristics of electron accelerators suitable for single pass free electron lasers are discussed. In particular, their beam qualities: energy, energy spread, current, emittance and bunch time structure are considered, in general and on a few examples. The matching of an electron beam to a tapered wiggler is finally dealt with in some detail.
A Sullivanian Approach to Supervision

Sullivan had many specific, practical ideas about how to approach the very difficult, schizoid, or borderline patient. As a result, students brought to him their sickest and most troublesome patients who either would not talk or could not explain in any clear, relevant way what had happened to them as they grew up or precisely how they felt about significant issues. Sullivan felt that schizoid patients had often been raised in such an atmosphere of ambiguity and double-talk—where deep feelings were denied or explained away by meaningless chatter— that they were led away from experiencing the truths of their world. Facts that they may have once seen clearly in early childhood were later buried or distorted to fit in with adult needs and prescriptions. The result was a blurring of memory, a forgetting of episodes that failed to fit into family myths, and an apparent acceptance of rationalizations or untruths in order to please significant adults or at least to avoid their rage and threatening disapproval. Words ceased to have real meaning. Other people’s ideas could not be trusted, but one’s own perceptions were also suspect or dangerous. Without consensual validation or parental affirmation, clear observations and authentic feeling responses were repressed, resulting in vagueness, confusion, and amnesia.
Transition from hospital-based surgery

Abstract The purpose of this paper is to examine issues that are imperative to spinal surgeons when considering moving from a hospital-based surgery center to an outpatient surgery center. Before transitioning to outpatient surgery, surgeons must be aware of learning curves for procedures they intend to perform in this setting and ensure that they have achieved procedural proficiency in hospital settings first in order to promote patient safety. Surgeons must also carefully plan outpatient care protocols, taking into account proper patient selection, postoperative pain control, and complication management. The current literature suggests that experienced surgeons can successfully transition to outpatient-based surgery, with no significant differences in patient outcomes. However, the data is still limited and more investigation is necessary to characterize the initial outpatient surgical experience.
Multimer: validating multimodal, cognitive data in the city: towards a model of how the urban environment influences streetscape users

Multimer is a new technology that aims to provide a data-driven understanding of how humans cognitively and physically experience spatial environments. By multimodally measuring biosensor data to model how the built environment and its uses influence cognitive processes, Multimer aims to help space professionals like architects, workplace strategists, and urban planners make better design interventions. Multimer is perhaps the first spatial technology that collects biosensor data, like brainwave and heart rate data, and analyzes it with both spatiotemporal and neurophysiological tools.The Multimer mobile app can record data from several kinds of commonly available, inexpensive, wearable sensors, including EEG, ECG, pedometer, accelerometer, and gyroscope modules. The Multimer app also records user-entered information via its user interface and micro-surveys, then also combines all this data with a user's geo-location using GPS, beacons, and other location tools. Multimer's study platform displays all of this data in real-time at the individual and aggregate level. Multimer also validates the data by comparing the collected sensor and sentiment data in spatiotemporal contexts, and then it integrates the collected data with other data sets such as citizen reports, traffic data, and city amenities to provide actionable insights towards the evaluation and redesign of sites and spaces.This report presents preliminary results from the data validation process for a Multimer study of 101 subjects in New York City from August to October 2017. Ultimately, the aim of this study is to prototype a replicable, scalable model of how the built environment and the movement of traffic influence the neurophysiological state of pedestrians, cyclists, and drivers.
Hematolymphoid neoplasms are common in bone marrow biopsies performed for non-specific, diffuse marrow signal alterations on magnetic resonance imaging.

Abstract Aims MRI is an imaging modality used for a wide range of clinical indications. Occasionally, non-specific, diffuse T1 marrow signal alterations are identified, which may prompt a bone marrow biopsy (BM). However, there is little data on the clinicopathologic significance of this signal alteration. This study evaluated the frequency and nature of pathologic findings in BM performed to evaluate diffuse MRI T1 marrow signal alterations. Methods Records from January 2003 to May 2015 were searched for BM performed to evaluate abnormal MRIs. 179 cases were identified. Patients with nodular/destructive bone lesions on MRI or other imaging studies, or a previous diagnosed metastatic tumor or hematologic malignancy were excluded, resulting in 45 cases. Results The patients included 22 males and 23 females with a median age of 56 years. The location of the MRI T1 marrow signal alterations included spine, pelvis, knee, skull, femur, and arm. 19/45 patients had neoplasms identified in the BM. The remaining 26 patients had benign BM findings. There was a significant difference in hemoglobin values in patients with neoplastic versus benign BM findings (p = 0.037, unpaired Student's t -test). Conclusions Diffuse T1 marrow signal alterations on MRI should warrant a BM evaluation, as 42% of cases showed an underlying hematolymphoid neoplasm or metastatic tumor, even when patients with a known history of malignancy were excluded. When faced with a BM from a patient with a non-specific, diffuse MRI signal alteration, a pathologist should have a high index of suspicion for a malignant neoplasm, most often of hematopoietic/lymphoid type.
MP24-13 IMPACT OF THE 2012 UNITED STATES PREVENTIVE SERVICES TASK FORCE PROSTATE CANCER GUIDELINES ON PARTICIPATION IN A COUNTY WIDE FREE PROSTATE CANCER SCREENING PROGRAM

INTRODUCTION AND OBJECTIVES:The 2008 United States Preventive Services Task Force (USPSTF) guidelines recommended against screening patients older than 75 years with PSA testing. The recommendation in 2012 further expanded these criteria to recommend against screening for prostate cancer based on PSA in all patients. It remains unclear how these changes impact public perception and participation in screening programs. We sought to compare prostate cancer screening participation before and after the 2012 USPSTF guidelines.METHODS:We conduct a state funded, free to participants, prostate cancer screening program. All participants present with the desire to screen for prostate cancer with PSA. Our cohort consisted of 7, 950 voluntary screening encounters at various sites in our county. These sites consisted of our outpatient clinic, community health centers, churches, fire and police departments. Patients were divided into two groups and compared based on the major guideline change in May 2012: 1) June 2008 ...
The Incidence of Arterial and Venous Thrombosis in Antineutrophil Cytoplasmic Antibody–associated Vasculitis

To the Editor: We read with interest in The Journal of Rheumatology the article by Kang, et al , “High Incidence of Arterial and Venous Thrombosis in Antineutrophil Cytoplasmic Antibody-associated Vasculitis,”1 which reported a dramatically high incidence of arterial (ATE) and venous thrombosis events (VTE) in a hospital-based cohort of patients with antineutrophil cytoplasmic antibody–associated vasculitis (AAV). The study generally supports the previously reported increased risk of ATE and VTE in patients affected by AAV2–8, but has a number of inaccuracies and methodological issues that inflate the incidence rates of ATE and VTE. First, the authors compared clinical and laboratory features at baseline between groups defined by events that occurred during the followup (ATE vs no ATE, and VTE vs no VTE, Table 1 and … Address correspondence to Dr. D. Cornec, CHU Brest, Rheumatology, Boulevard Tanguy Prigent, Brest, 29609, France. E-mail: divi.cornec{at}chu-brest.fr
Public perceptions of autonomous vehicle safety: An international comparison

Abstract Autonomous vehicles (AVs) are envisioned to reduce road fatalities by switching control of safety-critical tasks from humans to machines. Realizing safety benefits on the ground depends on technological advancement as well as the scale and rate of AV adoption, which are influenced by public perceptions. Employing multilevel structural equation modeling, this paper explores differences in perceptions of AV safety across 33,958 individuals in 51 countries. At the individual level, young males report higher perceptions of current AV safety and predict fewer years until AVs are safe enough for them to use. Since young males are more likely to undertake risky driving behavior, their positivity towards AV safety could lead to more rapid manifestations of safety benefits. Urban, fully employed individuals with higher incomes and education levels also report fewer years until AVs are safe to use. The multilevel model identifies country-level effects after controlling for individual characteristics. Developed countries with greater motorization rates and lower road death rates tend to have greater awareness of AVs but are more pessimistic about their present and future safety. Individuals in developing countries that face greater road safety challenges, particularly involving 2- and 3-wheeled vehicles, predict fewer years until AVs will be safe enough for them to use. Higher AV safety perception among the most risk-taking road users and in developing countries coincide with sociodemographic groups and geographic areas facing the greatest road safety challenges and most in need of improvement, highlighting a potential opportunity to reduce the global disparity in road safety.
Short tandem repeat genotypes of samples from eleven populations comprising the FBI’s population database

Abstract Electronic DNA genotype data corresponding to eleven FBI relevant populations, typed with the expanded STR chemistries, are presented. Such data may prove valuable for population genetic studies and other forensic relevant inquiries.
Position: GDPR Compliance by Construction

New laws such as the European Union’s General Data Protection Regulation (GDPR) grant users unprecedented control over personal data stored and processed by businesses. Compliance can require expensive manual labor or retrofitting of existing systems, e.g., to handle data retrieval and removal requests. We argue for treating these new requirements as an opportunity for new system designs. These designs should make data ownership a first-class concern and achieve compliance with privacy legislation by construction. A compliant-by-construction system could build a shared database, with similar performance as current systems, from personal databases that let users contribute, audit, retrieve, and remove their personal data through easy-to-understand APIs. Realizing compliant-by-construction systems requires new cross-cutting abstractions that make data dependencies explicit and that augment classic data processing pipelines with ownership information.
GWAS significance thresholds for deep phenotyping studies can depend upon minor allele frequencies and sample size

An important issue affecting genome-wide association studies with deep phenotyping (multiple correlated phenotypes) is determining the suitable family-wise significance threshold. Straightforward family-wise correction (Bonferroni) of p 0.1), the permutation family-wise threshold was in close agreement with spectral decomposition methods. However, for less common SNPs (0.05 < MAF ≤ 0.1), the permutation threshold calculated over all SNPs was off by orders of magnitude. This applies to the number of individuals studied (here 777) but not to very much larger numbers. Based on these findings, we propose that the threshold to find a particular level of family-wise significance may need to be established using separate permutations of the actual data for several MAF bins.
Prevalence and predictors of donor milk programs among U.S. advanced neonatal care facilities.

(1) Determine national prevalence and predictors of donor milk programs among levels 2–4 advanced neonatal care facilities; (2) describe characteristics of donor milk programs. We randomly selected 120 U.S. hospitals with levels 2–4 advanced neonatal care facilities among each of four U.S. regions and surveyed the medical directors from July 2017 to November 2017 regarding donor milk use. We weighted responses according to the number of birthing hospitals in each region. Response rate was 213/480 (44%). Twenty-eight percent of level 2 and 88% of levels 3 and 4 neonatal care facilities had donor milk programs. Donor milk programs occurred more often in the South vs. Northeast (aOR 3.7 [1.1, 12.5] and less often in safety-net hospitals (≥75% Medicaid patients) vs. nonsafety-net hospitals (aOR 0.3 [0.1, 0.8]). In 2017, the vast majority of levels 3 and 4 neonatal care facilities had donor milk programs but disparities existed according to the safety-net hospital status and region.
Publishing Qualitative Research in the "Journal of Educational Psychology": Synthesizing Research Perspectives across Methodological Silos.

Although there is increasing recognition of the value of mixed-methods research, many publication spaces remain methodologically siloed, primarily publishing either qualitative or quantitative research studies. This methodological siloing presents a risk to coherence in the psychological sciences if qualitative and quantitative research traditions continue to exist and develop in disconnected spaces. In this commentary, we share our editorial experience while editing this special section via a case study of the process of publishing qualitative research in a traditionally quantitative publication venue. Our central goal is to describe how we aimed to increase contact between qualitative and quantitative research traditions during the editorial process and the unique challenges and opportunities that arose as a result. We hope that this case study of how the special section was produced will help spark discussion and debate about the roles of qualitative and quantitative methods in educational psychology and about how research communities can transform the ways in which they publish and evaluate research. (PsycINFO Database Record (c) 2020 APA, all rights reserved)
Hemoglobin: Structure, Function and Allostery

This chapter reviews how allosteric (heterotrophic) effectors and natural mutations impact hemoglobin (Hb) primary physiological function of oxygen binding and transport. First, an introduction about the structure of Hb is provided, including the ensemble of tense and relaxed Hb states and the dynamic equilibrium of Hb multistate. This is followed by a brief review of Hb variants with altered Hb structure and oxygen binding properties. Finally, a review of different endogenous and exogenous allosteric effectors of Hb is presented with particular emphasis on the atomic interactions of synthetic ligands with altered allosteric function of Hb that could potentially be harnessed for the treatment of diseases.
Comparison of therapy-related and de novo core binding factor acute myeloid leukemia: A bone marrow pathology group study.

This multi-institutional study retrospectively evaluated clinicopathologic and genetic characteristics in 351 patients with core-binding-factor acute myeloid leukemia (CBF-AML), comprising 69 therapy-related (t-CBF-AML) and 282 de novo cases. The T-CBF-AML patients were older, had lower WBC counts, and slightly higher hemoglobin than patients with de novo disease. Secondary cytogenetic abnormalities were more frequent in patients with de novo disease than t-CBF-AML (57.1% vs 41.1%, P = .026). Patients with secondary cytogenetic abnormalities had longer overall survival (OS) than those without abnormalities (median 190 vs 87 months, P = .021); trisomy 8, trisomy 22, and loss of the X or Y chromosome were associated with longer OS. In the 165 cases performed of targeted gene sequencing, pathogenic mutations were detected in 75.7% of cases, and were more frequent in de novo than in therapy-related disease (P = .013). Mutations were found in N/KRAS (37.0%), FLT3 (27.8%), KIT (17.2%), TET2 (4.9%), and ASXL1 (3.9%). The TET2 mutations were associated with shorter OS (P = .012) while N/KRAS mutation was associated with longer OS in t(8;21) AML patients (P = .001). The KIT mutation did not show prognostic significance in this cohort. Although they received similar therapy, t-CBF-AML patients had shorter OS than de novo patients (median 69 vs 190 months, P = .038). In multivariate analysis of all patients, older age and absence of any secondary cytogenetic abnormalities were significant predictors of shorter OS. Among the t-CBF-AML subset, age and hemoglobin were significant on multivariate analysis. This study demonstrated that although de novo and t-CBF-AML patients share many features, t-CBF-AML patients have worse clinical outcome than de novo patients.
US Department of Agriculture Summer Meals Program: what's hot?

This article provides an overview of the US Department of Agriculture Summer Meals Program (SMP) and highlights opportunities to strengthen SMP's public health impacts We also discuss initial SMP implications of 2 relevant policy provisions of the Families First Coronavirus Response Act (P L 116-127), signed into law on March 18, 2020 Ensuring access to summer meals among high-risk students can provide (1) supplemental nutrition assistance to families that helps address food insecurity during the summer months when there are no school meals, (2) healthy meals in structured settings that might help reduce obesity risk, and (3) support to other programs that offer other benefits such as education, physical activity, or job training
Conservative management of neck and thoracic pain in an adult with neurofibromatosis-1.

Objective To describe the case of a 21-year-old female with previously diagnosed neurofibromatosis type 1 (NF1) with neck, scapular, lumbar, and temporomandibular discomfort along with headaches. Clinical features The patient had chronic tightness and pain in the cervicothoracic region as well as pain in the lumbar spine at the site of prior neurofibroma removal. Radiographs demonstrated multilevel osseous changes. In addition to NF-1, she was diagnosed with cervical myalgia, tension-type headaches, and chronic temporomandibular joint disorder. Intervention and outcomes Treatment consisted of a course of manual therapy including cervical flexiondistraction, myofascial release, patient education on workplace ergonomics, and an at-home active care plan. The patient experienced a reduction in pain and headache frequency. Summary Manual therapy in the form of cervical flexion-distraction with myofascial release and education on workplace ergonomics were effective in reducing neck and thoracic pain as well as reducing headache frequency in a 21-year-old with NF-1.
A postreplicative C5-cytosine hypermodification triggered by bacteriophage methyltransferase and hydroxylase.

Viruses of bacteria, also known as bacteriophages, harbor the greatest diversity of DNA modifications identified to date. To fight against restriction endonucleases of their hosts, bacteriophages modify their genomic DNA through introduction of various moieties including amino acids, polyamines, and sugars (1, 2). A series of transformations are involved in DNA base modification. Followed by formation of hydroxymethyl pyrimidine nucleotides, which are utilized by DNA polymerase, replication and postreplicative modifications furnish installation of these moieties onto the DNA polymer (3⇓⇓⇓–7). Burke et al. (8) show that bacteriophages resort to C5-cytosine methyltransferase (C5-MT) and 5-methylcytosine dioxygenase ten-eleven translocation enzyme (TET) as an alternative mechanism to postreplicatively form hydroxymethylcytosine on DNA. The bacteriophage TET enables site-specific hydroxylation of 5-methylcytosine (5mC), installed by C5-MT, to produce 5-hydroxymethylcytosine (5hmC). Through bioinformatic screening, the authors identify and characterize tailoring enzymes, such as glycosyltransferases, that collaborate with phage C5-MT and TET to … [↵][1]1To whom correspondence may be addressed. Email: wchang6{at}ncsu.edu. [1]: #xref-corresp-1-1
Prevalence of Malignancy on Contrast-Enhanced Computed Tomography of the Abdomen and Pelvis in Patients With Unexplained, Unintentional Weight Loss.

OBJECTIVE: The aim of the study was to evaluate the prevalence of malignancy on contrast-enhanced computed tomography (CT) of the abdomen and pelvis in patients with unexplained, unintentional weight loss (UUWL). METHODS: This is a retrospective review of 999 adult outpatient contrast-enhanced CT of the abdomen and pelvis for UUWL. Patients were stratified into 2 groups: (1) weight loss only (WLO, n = 222) and (2) UUWL with additional symptoms (UUWL+, n = 777). χ2 test was performed to compare malignancy detection rate in the WLO and UUWL+ groups. RESULTS: Prevalence of malignancy was 5.3% (95% confidence interval [CI], 4.2%-7.1%; 55 of 999). Prevalence of malignancy in the WLO group was 2.3% (95% CI, 0.7%-5.2%; 5 of 222), lower than the prevalence of 6.2% (95% CI, 4.6%-8.1%; 48 of 777) in the UUWL+ group (P = 0.02). Prevalence of malignancy was lower in patients younger than 60 years in all patients and in the UUWL+ subgroup (P < 0.01 in both cases). CONCLUSIONS: There is low prevalence of malignancy on contrast-enhanced CT of the abdomen and pelvis in patients with UUWL, particularly in younger patients and those without additional symptoms.
Did the Economic Stimulus Act Really Stimulate the Economy

The American Recovery and Reinvestment Act of 2009 is a massive piece of legislation that was enacted on the heels of the most significant financial crisis of modern time. It was aimed at avoiding a depression. The provisions of the Act were aimed at calming the panic that had gripped investors and the American public and restoring trust in the financial industry. It was also aimed at stimulating the economy and creating jobs. There are many who question whether the Act really worked and whether tax incentive programs can effectively be utilized to stimulate the economy. In this paper, we examine the components of the Act as it relates to small businesses and individuals and analyze whether these incentives actually worked or did not work. We make recommendations and suggest strategies that can be utilized to ensure the success of future initiatives.
Relating PAC damping to EFG fluctuation rates through the PAC relaxation peak

A perturbed angular correlation (PAC) experiment that measures dynamic damping also needs information about the fundamental quadrupole frequency to relate the damping as a function of temperature to the EFG fluctuation rate. When the experiment is unable to access slow electric field gradient (EFG) fluctuations that show the fundamental quadrupole frequency directly, one needs additional information to determine the hyperfine field parameters and thereby the connection between observed damping and EFG fluctuation rates. One way to solve this problem is to estimate the hyperfine parameters from the fluctuation rate for maximum damping (i.e. at the relaxation peak) or from the rate of maximum damping. This work relates both the maximum damping rate and the fluctuation rate at the relaxation peak to EFG magnitudes (or quadrupole frequencies) for five dynamic N-state symmetric models of fluctuating EFGs.
The Synthesis of Ketones from Acid Halides and Organometallic Compounds of Magnesium, Zinc, and Cadmium

The synthesis of ketones from acid halides and organometallic compounds may be represented by the following equation: Freund fist observed the formation of ketones from simple acid chlorides and dimethyl- and diethyl-zinc. Organometallic compounds of sodium, copper, magnesium, cadmium, mercury, aluminum, tin, and lead have also been found to give this reaction. Only organozinc, organocadmium, and organomagnesium reagents have been found to be generally useful for such syntheses, however. These reactions are reviewed in this chapter. Keywords: ketones; acid halides; organometallic zinc; organometallic cadmium; organometallic magnesium; grignard reagents; other syntheses; experimental procedures
Evaluation of Teat Coverage Persistency and Teat Health for Four Dry Period Persistent Barrier Teat Sealant Dips

Mastitis research has shown that 40-50% of intramammary infections (IMI) are contracted during the dry or non-lactating period with the greatest percentages of these occurring during the first and last two weeks of the dry period. The ability to develop and apply external persistent barrier teat dip products (like a liquid bandage) that can persist for these 1 week periods could decrease IMI, thus improving animal health and performance, and product quality and safety. The objective of this study was to evaluate and compare 4 persistent barrier dry cow teat sealant dips with particular interest and comparisons of dip persistency in providing teat end protection, and overall teat end and skin health. Cows dipped with all dips had significantly greater persistency and protection compared to previous 4 trials (last 2 years). All dips were easy to apply and showed excellent teat health. Films were very consistent and very flexible over time (limited ripping, shredding or flaking). Overall, Dip B showed the greatest persistency in the first 4 days post dipping which is a very critical time period.
Web-based Training

The purpose of this book is to provide readers with information related to design, development, delivery, management, implementation, and evaluation aspects of WBT. The book is divided into three main Sections - Section I: Web-Based Training: Introduction; Section II: Web-Based Training: Design and Development Perspectives; and Section III: Web-Based Training: Implementation and Evaluation Perspectives. The book, containing 63 chapters, contributed by more than one hundred authors from throughout the world, explains how to use the Internet's World Wide Web for training in corporations, government agencies, and educational institutions.
A Simple Inter‐Class Distance Parameter for Predictive SAR/QSAR Models

The objective of many SAR or QSAR experiments is to develop statistical classification models that can separate chemicals into classes of “active” or “inactive” under a toxicological endpoint. Several existing statistical methods are often used to provide either quantitative or qualitative measures of the distance or separation between the active and inactive classes, including sensitivity, accuracy and the receiver-operator characteristic (ROC) curve. The present study proposes a simple “distance parameter” as a measure of the relative separation between classes of “active” and “inactive” chemicals. The distance parameter can be used alone or with existing statistical measures for both the optimization of a single model with respect to maximizing sensitivity and specificity, and also in the selection of the best model from among alternative models.
Feeding condition and the relative contribution of different dopamine receptor subtypes to the discriminative stimulus effects of cocaine in rats.

Rationale The contribution of dopamine receptor subtypes in mediating the discriminative stimulus effects of cocaine is not fully established. Many drug discrimination studies use food to maintain responding, necessitating food restriction, which can alter drug effects.
The intensity coherence function of time for partially saturated acoustic propagation through ocean internal waves.

The intensity coherence function of time for partially saturated acoustic propagation through internal waves is calculated with a method that is improved over previous treatments. Two specific improvements are introduced: the usual expansion in (1/ΛΦ2) is carried out to a higher order, and then the terms of that expansion are calculated with a new perturbative method. The method is applied to propagation without a sound channel, for both phase-screen and continuous-medium cases. The validity of the new perturbative method is estimated by calculating the next order error terms. Accuracies at the few-percent level are found. The new analytic formulas are also corroborated with numerical integration. Finally, the method is applied to a specific ocean-acoustic experiment [Azores Fixed Acoustic Range (AFAR)]. In order to achieve good agreement with experiment it will be necessary to add an accurate treatment of the sound channel to the present perturbation method.
Purification of human monocytes: isolation and collection of large numbers of peripheral blood monocytes.

Abstract Monocytes from human peripheral blood were purified by elutriation centrifugation. Up to 1.5 × 10 9 peripheral blood mononuclear cells could be separated in isotonic media to yield 6–8 × 10 8 lymphocytes with 95% purity and 1.5–2.5 × 10 8 monocytes with greater than 90% purity. The temperature at which elutriation was performed determined the purity of the monocyte fraction. Both the lymphocyte and monocyte fractions were characterized by cell sizing and histochemical staining. Most of the myeloperoxidase positive mononuclear cells (monocytes) has a modal volume of 378 μ m 3 while 10% were within the modal volume of the lymphocytes, 131 μ m 3 . Thus, there are two populations of cells with histochemical properties of monocytes which are separable by size. The collection and isolation of human peripheral blood monocyte populations in large numbers will facilitate studies of their functional characteristics.
Cocrystals of Piroxicam with Carboxylic Acids

A crystal engineering method was used to investigate cocrystal formation of piroxicam with pharmaceutically acceptable carboxylic acids. Forming cocrystals of piroxicam can potentially result in solid forms with increased bioavailability. A total of 50 unique cocrystals containing piroxicam and a guest carboxylic acid were identified in screening experiments. Each of the 23 guest molecules tested formed at least one cocrystal with piroxicam. In addition, the three known polymorphs of piroxicam were observed. Raman data for the piroxicam cocrystals can be sorted into three distinct groups based on spectral similarity. These groups are differentiated by the piroxicam tautomer present in the cocrystal and the presence or absence of a strong hydrogen bond donor interacting with piroxicam's amide carbonyl group. X-ray powder diffraction data revealed six isostructural piroxicam cocrystals. Single-crystal structure determination revealed that this isostructural series is a host−guest system in which the piroxic...