cssi/SciSciGPT-SciSciNet huggingface dataset:
Violence and counterviolence: the need for a children's domestic exchange.

What is commonly talked about as black violence in this country might more accurately be described as the black's counterviolent response to white violence done to him. This paper analyzes the many forms of white violence, uses the suicide analogy to understand the current stage of black counterviolence, and proposes a remedy that conforms to the “living law'' habits of the American people.
Millimeter emission lines in Orion A

During the course of a search of Orion A for signals from three large organic molecules, several millimeter-wave lines from known interstellar molecules were observed. Results are reported for observations of methanol (CH3OH), methyl cyanide (CH3CN), methyl acetylene (CH3CCH), acetaldehyde (CH3CHO) and (Si-29)O. Emission signals from two hydrogen recombination lines (H41-alpha and H42-alpha) detected from the H II region of Orion A are also reported. Negative results were obtained for several millimeter-wave transitions of ethylene oxide, acetone, and cyclopropenone.
Detoxification of the phytoalexin pisatin by a fungal cytochrome P-450

The fungus Nectria haematococca, a pathogen of garden pea (Pisum sativum), can demethylate pisatin, an antimicrobial compound synthesized by infected pea tissue. The phenolic product is less toxic than pisatin to many microorganisms. Cell extracts catalyzing pisatin demethylation were obtained from N. haematococca, and the properties of the reaction were examined. The enzyme activity was greatest in the high-speed pellet fraction, in which rates up to 20 nmol/min/mg protein were observed. The Km for pisatin was relatively low, less than 5 μm. The reaction was dependent on NADPH, which could not be replaced by any other cofactor tested. However, in the presence of NADPH, NADH increased the rate of demethylation. Oxygen uptake by the enzyme was stimulated by addition of pisatin, the increment of oxygen utilization being approximately equimolar with pisatin added. Formaldehyde was a product of the reaction. The effects of various inhibitors were tested to determine whether this reaction is mediated by cytochrome P-450. The respiratory inhibitors KCN (1 mm) and antimycin A strongly inhibited the demethylation of pisatin by intact cells of the fungus, but not by the NADPH-supplemented enzyme. The cytochrome P-450 inhibitors SKF 525-A and 1-(2-isopropylphenyl)imidazole inhibited demethylation both in whole cells and in the enzyme preparation, though the latter compound was effective only at high concentrations. Most other cytochrome P-450 inhibitors tested had little effect. However the reaction was quite sensitive to CO, and this inhibition was readily reversed by light at wavelengths near 450 nm. It is concluded that pisatin demethylase is a cytochrome P-450 monooxygenase.
Differential effect of urea on the migration of collagenous and non-collagenous polypeptides in SDS gels: basis for their separation by two-dimensional electrophoresis.

Abstract The α1(I), α2, and α1(III) collagen chains are retarded in urea-SDS gels relative to their migration in SDS gels without urea (Hayashi and Nagai, 1979). This effect of urea provides the rationale for the two-dimensional separation of collagenous from non-collagenous polypeptides by two-dimensional electrophoresis consisting of urea-SDS in one dimension and SDS alone in the other dimension. In this system, non-collagenous polypeptides align themselves diagonally across the separation plane while each class of α chain with its homologous crosslinked components, forms a separate and distinct alignment at an angle to the central diagonal.
Determination of reasons for cessation of participation in serial plasmapheresis programs.

BACKGROUND: An earlier retrospective review of records of over 500 remunerated donors participating in plasmapheresis programs at various levels of frequency for 10 or more years showed that there were no significant differences in mean values for total protein, microhematocrit, body weight, and individual serum proteins, regardless of the number of annualized donations for each of four frequency groups. Although these data demonstrated that donors undergoing serial plasmapheresis for 10 or more years were able to successfully continue in such programs, the review did not include information relative to individuals who had discontinued their participation. The current study investigated reasons that donors drop out of plasmapheresis programs. STUDY DESIGN AND METHODS: Donors who had successfully undergone plasmapheresis at least 20 times within a recent 6-month period but who had failed to appear for at least 30 days subsequent to their last donation were defined as potential subjects for the study. Staff members at the participating center contacted the donors, inviting them to return to the center to participate in a survey. Donors were also asked for a blood sample upon their arrival at the plasma center; samples were tested for total and various serum proteins. RESULTS: A total of 409 eligible subjects appeared at the centers for the survey, and 375 blood samples were analyzed for proteins. Survey responses indicated that the predominant reasons for an end to participation were socioeconomic in nature. Serum protein findings were compared to the most recent results obtained while the donors were active in the plasmapheresis program. Slightly higher mean serum protein findings were seen for samples collected on the survey date. CONCLUSION: A review of the aggregate responses obtained in the survey indicates that the predominant reasons that donors ceased to participate were not health-related: the remuneration was no longer needed, and work schedule conflicts, time constraints, and relocation were frequently cited.
Gonadotrope- and thyrotrope-specific expression of the human and bovine glycoprotein hormone alpha-subunit genes is regulated by distinct cis-acting elements.

The proximal 5'-flanking region of the alpha-subunit gene from humans and cattle confers pituitary-specific expression to heterologous reporter genes in transgenic mice. To investigate whether these promoter regions also contain the necessary regulatory elements for cell-specific expression and hormonal regulation, we used three independent lines of transgenic mice. Two lines of transgenic mice contained chimeric genes consisting of either 1.6 kilobasepairs (kbp) of human or 3 15 basepairs of bovine alpha-subunit proximal 5'-flanking sequence linked to the bacterial gene encoding chloramphenicol acetyltransferase (CAT). A third line of transgenic mice contained the proximal 1.6 kbp of 5'-flanking sequence of the human alpha-subunit gene linked to the bacterial lacZ gene encoding beta-galactosidase (beta gal; H alpha beta gal transgenic mice). Hormonal replacement paradigms indicate that both human and bovine alpha CAT transgenes are regulated by GnRH, suggesting that their expression occurs in gonadotrope...
Binding and hydrolysis of TNP-ATP by Escherichia coli F1-ATPase.

It had previously been suggested that Vmax hydrolysis rate of 2', 3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate (TNP-ATP) by F1-ATPase required filling of only two catalytic sites on the enzyme (Grubmeyer, C., and Penefsky, H. S. (1981) J. Biol. Chem. 256, 3718-3727), whereas recently it was shown that Vmax rate of ATP hydrolysis requires that all three catalytic sites are filled (Weber, J., Wilke-Mounts, S., Lee, R. S. F., Grell, E., and Senior, A. E. (1993) J. Biol. Chem. 268, 20126-20133). To resolve this apparent discrepancy, we measured equilibrium binding and hydrolysis of MgTNP-ATP under identical conditions, using betaY331W mutant Escherichia coli F1-ATPase, in which the genetically engineered tryptophan provides a direct fluorescent probe of catalytic site occupancy. We found that MgTNP-ATP hydrolysis at Vmax rate did require filling of all three catalytic sites, but in contrast to the situation with MgATP, "bisite hydrolysis" of MgTNP-ATP amounted to a substantial fraction (approximately 40%) of Vmax. Binding of MgTNP-ATP to the three catalytic sites showed strong binding cooperativity (Kd1 < 1 nm, Kd2 = 23 nm, Kd3 = 1.4 microM). Free TNP-ATP (i.e. in presence of EDTA) bound to all three catalytic sites with lower affinity but was not hydrolyzed. These data emphasize that the presence of Mg2+ is critical for cooperativity of substrate binding, formation of the very high affinity first catalytic site, and hydrolytic activity in F1-ATPases and that these three properties are strongly correlated.
Infrared spectroscopy of simple molecules in liquid-argon solution—III. The determination of the anharmonic force constants of OCS

Abstract The i.r. spectrum of OCS has been measured in liquid-argon solution and thirty three vibrational bands have been assigned. The vibrational wavenumbers, measured in liquid-argon solution, have been used to calculate the anharmonic (cubic and quartic) internal-coordinate force constants of OCS; the results are compared with previous calculations using gas-phase data.
Gert on Subjective Practical Rationality

The purpose of this paper is to consider Joshua Gert’s novel view of subjective practical rationality in his book Brute Rationality. After briefly outlining the account, I present two objections to his view and then consider his own objections to a rival approach to understanding subjective rationality which I take to be much more plausible.
MORAL THEME COMPREHENSION IN THIRD GRADERS, FIFTH GRADERS, AND COLLEGE STUDENTS

Abstract Proponents of character education (e.g., Bennett, author of The Book of Virtues) argue that children should hear moral stories in order to develop moral character. But do children necessarily understand the moral message of a narrative? Third and fifth grade students and adults were tested for their ability to apprehend the moral messages in three stories selected from The Book of Virtues and other sources. After listening and reading along with three moral stories, participants were asked to generate the message, select the message from a list of possible messages and select a vignette with the same message from three possible vignettes. Subjects also heard and read a non‐moral story and answered comprehension questions about it. Results indicate a developmental difference in moral theme understanding unexplained by reading comprehension scores alone.
Experimental verification of manufacturing error pattern and its utilization in form tolerance sampling

This paper presents an adaptive sampling procedure, which uses manufacturing surface error patterns and optimization search methods for reducing sample size, while maintaining high accuracy. The methodology is demonstrated with straightness and flatness evaluation. Two manufacturing processes, end and face milling are used to fabricate plates used for modeling and subsequent testing. Respective surface errors for each process are quantified and validated using previously published models. The initial points for sampling are identified through such a characterization of the process and its affect on the workpiece. These sampled points are fit using the least-squares method to complete the form evaluation. Points are added to the initial set using optimization search heuristics. The final tolerance value obtained is compared with that obtained from a large population sample to check the accuracy. With such an adaptive approach, it is proposed that the number of points sampled is potentially less than that which would be expected to achieve the same level of accuracy using traditional sampling methods. This paper demonstrates the error modeling and validation aspects of this adaptive sampling procedure.
Immunocytochemical Studies of Substance P and Met-Enkephalin in the Basal Ganglia and Substantia Nigra in Huntington's, Parkinson's and Alzheimer's Diseases

Immunocytochemical studies of the distribution and intensity of Substance P and Met-enkephalin staining in the basal ganglia and substantia nigra were carried out in five cases each of brains from patients with Huntington's disease, Parkinson's disease, Alzheimer's disease, and normal controls. The usefulness of the peroxidase-antiperoxidase method for human autopsy material was confirmed. Substance P and Met-enkephalin fibers were distributed in essentially the same pattern as described in experimental animals and in human brains. In Huntington's disease brains decreased Substance P staining was found in the internal globus pallidus and the substantia nigra, in agreement with radioimmunoassay studies by others. Met-enkephalin staining in the external globus pallidus was of normal intensity, although present within a shrunken area. In Parkinson's and Alzheimer's diseases there was intense immunoreactivity for Substance P in the globus pallidus and substantia nigra, and for Met-enkephalin in the globus pallidus, at variance with reported decreases in Parkinson's disease by radioimmunoassay, but in essential agreement with other immunocytochemical studies. Immunocytochemical methods complement radioimmunoassays of human brain and may help in mapping neuropeptidergic pathways and in pinpointing abnormalities in these pathways in basal ganglia disorders.
INTERNATIONAL HUMAN RIGHTS STANDARDS: GUIDELINES FOR THE WORLD'S POLICE OFFICERS

Police agencies throughout the world need to be concerned about the violation of human rights by their police officers. The police in all countries are accused of violating the human rights of the citizens they serve. As members of the United Nations, the governments of the world must enforce the human rights standards established by the United Nations and agreed on by most member nations of this world body. This article reviews the definition of human rights and standards pertaining to police officers that are known as international human rights instruments. The methods suggested by the United Nations to implement human rights, along with measures to monitor human rights violations and sanctions for violation of human rights by police officers, are covered in the discussion.
129Xe and131Xe NMR of xenon on silica surfaces at 77 K

Little is known about129Xe NMR spectral features and spin-lattice relaxation behavior, and the dynamics of xenon atoms, for xenon adsorbed on solid surfaces at cryogenic temperatures (≤77 K), where exchange with gas-phase atoms is not a significant complication. We report129Xe NMR experiments at 9,4 T that provide such information for xenon adsorbed onto the hydroxylated surface of a number of microporous silica samples at 77 K. A convenient design for these cryogenic experiments is described. Dynamics of surface-adsorbed xenon atoms on the time scale of seconds can be observed by129Xe NMR hole-burning experiments; much slower dynamics occurring over hours and days are evidenced from changes with time of the129Xe NMR chemical shifts. The peak maxima occur in the region ca. 180–316 ppm, considerably downfield of129Xe shifts previously reported on surfaces at higher temperatures, and closer to the shift of xenon bulk solid (316.4±1 ppm). The129Xe spin-lattice relaxation timesT1 range over five orders of magnitude; possible explanations for both nonexponential relaxation behavior and extremely shortT1 values (35 ms) are discussed. Preliminary131Xe and1H NMR results are presented, as well as a method for greatly increasing the sensitivity of129Xe NMR detection at low temperatures by using closely-spaced trains of rf pulses.
Calculation of the electron affinities of the chromophores involved in photosynthesis

We examine the electron affinities (EA) of the postulated electron acceptor chromophores of the photosynthetic reaction center in R. Viridis. We estimate a difference of EA`s between bacteriochlorophyll-b and bacteriopheophytin-b of 0.19eV, in excellent agreement with the experimental value reported at 0.20 eV. We estimate this difference in situ at 0.42 eV, compared to an experimental estimate of 0.34 eV. These results support those of Thompson and Zerner that in the absence of specific interactions between BChl-L-side and the environment, electron transfer to BChl-L-side might not be directly involved in the overall electron transfer process. 42 refs., 2 figs., 3 tabs.
Quantum effects, thermal statistics and reliability of nanoscale molecular and semiconductor devices

The reliability of nanoscale molecular and semiconductor devices is analysed in terms of generalized error prediction algorithms. The authors statistical procedures predict the reliability of a device based on the assignment of three independent variables. The first variable, P(1) is the probability of determining the state of the device if only one molecule (or information carrier) is present. The second variable N is the number of molecules (or information carriers) operating to assign the state of the device (that is, the number of quantized entities within the ensemble). The third variable xi lim is the limiting logarithmic reliability factor which is a function of the entire system and the environment in which it must operate. Statistical procedures are also presented which allow one to determine the total system reliability based on the assignment of the desired mean-time-between-failures (DMTBF) and the individual reliabilities of the components. Sample reliability analyses are carried out on three devices to determine the minimum number of molecules N that must be included in the ensemble to achieve an error probability of less than 10-10.
How do leaf hydraulics limit stomatal conductance at high water vapour pressure deficits

A reduction in leaf stomatal conductance (g) with increasing leaf-to-air difference in water vapour pressure (D) is nearly ubiquitous. Ecological comparisons of sensitivity have led to the hypothesis that the reduction in g with increasing D serves to maintain leaf water potentials above those that would cause loss of hydraulic conductance. A reduction in leaf water potential is commonly hypothesized to cause stomatal closure at high D. The importance of these particular hydraulic factors was tested by exposing Abutilon theophrasti, Glycine max, Gossypium hirsutum and Xanthium strumarium to D high enough to reduce g and then decreasing ambient carbon dioxide concentration ([CO2]), and observing the resulting changes in g, transpiration rate and leaf water potential, and their reversibility. Reducing the [CO2] at high D increased g and transpiration rate and lowered leaf water potential. The abnormally high transpiration rates did not result in reductions in hydraulic conductance. Results indicate that low water potential effects on g at high D could be overcome by low [CO2], and that even lower leaf water potentials did not cause a reduction in hydraulic conductance in these well-watered plants. Reduced g at high D in these species resulted primarily from increased stomatal sensitivity to [CO2] at high D, and this increased sensitivity may mediate stomatal responses to leaf hydraulics at high D.
Nonlinear steady-state response of a rotor-support system

A numerical method is presented to determine the steady-state nonlinear response of a rotor-support system due to deadband and rubbing using discrete Fourier transformation and inverse discrete Fourier transformation. Damaging subharmonic and superharmonic responses are found to occur in presence of a side force. The calculated results agree with the general trends which have been observed experimentally by other investigators. The effects of selected nondimensionalized parameters on rotor response are studied.
Health care-associated infections in children after cardiac surgery.

Few recent studies have assessed the epidemiology of health care-associated infections (HAIs) in the pediatric population after cardiac surgery. A retrospective cohort study was performed to assess the epidemiology of several types of HAIs in children 18 years of age or younger undergoing cardiac surgery from July 2010 to June 2012. Potential pre-, intra-, and postoperative risk factors, including adherence to the perioperative antibiotic prophylaxis regimen at the authors’ hospital, were assessed by multivariable analysis using Poisson regression models. Microorganisms associated with HAIs and their susceptibility patterns were described. Overall, 634 surgeries were performed, 38 (6 %) of which were complicated by an HAI occurring within 90 days after surgery. The HAIs included 7 central line-associated bloodstream infections (CLABSIs), 12 non-CLABSI bacteremias, 6 episodes of early postoperative infective endocarditis (IE), 9 surgical-site infections (SSIs), and 4 ventilator-associated pneumonias (VAPs). Mechanical ventilation (rate ratio [RR] 1.07 per day; 95 % confidence interval [CI] 1.03–1.11; p = 0.0002), postoperative transfusion of blood products (RR 3.12; 95 %, CI 1.38–7.06; p = 0.0062), postoperative steroid use (RR 3.32; 95 % CI 1.56–7.02; p = 0.0018), and continuation of antibiotic prophylaxis longer than 48 h after surgery (RR 2.56; 95 % CI 1.31–5.03; p = 0.0062) were associated with HAIs. Overall, 66.7 % of the pathogens associated with SSIs were susceptible to cefazolin, the perioperative antibiotic prophylaxis used by the authors’ hospital. In conclusion, HAIs occurred after 6 % of cardiac surgeries. Bacteremia and CLABSI were the most common. This study identified several potentially modifiable risk factors that suggest interventions. Further studies should assess the role of improving adherence to perioperative antibiotic prophylaxis, the age of transfused red blood cells, and evidence-based guidelines for postoperative steroids.
Her-2/neu expression in ovarian cancer: pre- and postexposure to platinum chemotherapy.

Abstract Objective Our objective was to determine if the level of Her-2/neu expression in advanced ovarian cancer changed after platinum-based chemotherapy. Methods Tissue samples from 43 patients who had surgery for ovarian cancer between 1991 and 2001 at the Mayo Clinic were stained for Her-2/neu expression using the DAKO kit and reviewed independently by two pathologists. Patient charts were reviewed for demographic data, clinical course, chemotherapy, and survival times. Results Her-2/neu expression was 0 in 30 patients (69.76%), 1+ in 12 patients (27.9%), and 3+ in 1 patient (2.32%) before chemotherapy. After platinum chemotherapy, Her-2/neu expression changed from 0 to 1+ in 7 patients, from 1+ to 0 in 4 patients, 0 to 2+ in 1 patient, and 1+ to 2+ in 2 patients and no change was seen in 29 patients. Both pathologists agreed in all instances when the score was 0 or 1+ and disagreed in two instances between a negative and a weakly positive staining. Conclusions Our findings indicate a low level of overexpression of Her-2/neu at the time of primary diagnosis of epithelial ovarian cancer. Relapsing tumors show no significant change in the intensity of Her-2/neu expression after platinum-based chemotherapy. Further prospective studies are needed to confirm these findings and to ascertain whether platinum chemotherapy indeed has no effect on Her-2/neu expression.
Histone acetylation: chromatin in action

Histone acetylation acts as a landmark and determinant for chromatin function. Active roles in the transcription and assembly of chromatin have been discovered for histone acetyltransferases and deacetylases. This review highlights these roles and discusses their significance for the maintenance of cell differentiation.
Computation of energy release rate components by the use of near-tip stress and displacement distributions

Abstract A simple method, called the two-term parameter technique, is introduced for the computation of the energy release rate in specimens made of composite materials. A cracked lap shear specimen with a zero degree stacking sequence was employed. The mode II energy release rate, calculated by the two-term parameter technique, was compared with that determined by using the crack closure method. The results show that the two-term parameter technique is very comparable to the crack closure method and does not require exact information of the stress and displacement distributions at the crack tip to get the energy release rate. Moreover, it is shown that while the crack closure method depends on the crack extension size, the two-term parameter technique is less affected by it.
Trichinella spiralis: Acceleration and inhibition of cyst calcification in rats

Daily administration of vitamin D3 (75,000 IU/kg b.wt) for 7 days acceleratedTrichinella spiralis cyst calcification in rats with a 14-week-old infection. When disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP) was administered (50 mg/kg b.wt) from 2 days before until 2 days after vitamin D3 treatment, cyst calcification was inhibited. Thus, the ability to inhibitT. spiralis calcification has been demonstrated for the first time.
The Stretta procedure for the treatment of GERD: a registry of 558 patients.

Purpose: To evaluate gastroesophageal reflux disease (GERD) symptoms, patient satisfaction, and antisecretory drug use in a large group of GERD patients treated with the Stretta procedure (endoluminal temperature-controlled radiofrequency energy for the treatment of GERD) at multiple centers since February 1999. Methods: All subjects provided informed consent. A health care provider from each institution administered a standardized GERD survey to patients who had undergone Stretta. Subjects provided (at baseline and follow-up) (1) GERD severity (none, mild, moderate, severe), (2) percentage of GERD symptom control, (3) satisfaction, and (4) antisecretory medication use. Outcomes were compared with the McNemar test, paired t test, and Wilcoxon signed rank test. Results: Surveys of 558 patients were evaluated (33 institutions, mean follow-up of 8 months). Most patients (76%) were dissatisfied with baseline antisecretory therapy for GERD. After treatment, onset of GERD relief was less than 2 months (68.7%) o...
Chromosomes and causation of human cancer and leukemia. XIII. An evaluation of karyotypic findings in erythroleukemia

This study was an attempt at defining the cytogenetic features of erythroleukemia (EL), particularly as related to the group of AML patients with MAKA (major karyotypic abnormalities), which generally were caused by three or more cellular events of translocation or nondisjunction. Eight of the 17 patients with MAKA had a diagnosis of EL or possible EL. In most cases, MAKA was featured by hypodiploidy, karyotypic instability, and polyploidy in the leukemic cells. The most common abnormalities were loss of B or G group chromosomes and gain of a no. 16 or one or more marker chromosomes. Each of the markers of 2q+, Dq+, mar(A2, st), mar(C12, M), r(?F) and minute metacentric and acentric markers was observed in two or more patients. The extent of polyploidy seemed to be correlated with the proportion of erythroid precursor cells in the marrow and with the karyotypic instability. Since the patients exhibited the same chromosomal features, whether or not they had a diagnosis of EL or possible EL, and since patients without such a diagnosis also had cytologic suggestions of EL, a close relation of MAKA to EL is assumed. It is believed that patients with MAKA constitute one of the three chromosomally classifiable groups of EL.
Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific rice cross.

To detect QTLs controlling traits of agronomic importance in rice, two elite homozygous lines 9024 and LH422, which represent the indica and japonica subspecies of rice (Oryza sativa), were crossed. Subsequently a modified single-seed-descent procedure was employed to produce 194 recombinant inbred lines (F8). The 194 lines were genotyped at 141 RFLP marker loci and evaluated in a field trial for 13 quantitative traits including grain yield. Transgressive segregants were observed for all traits examined. The number of significant QTLs (LOD ⩾ 2.0) detected affecting each trait ranged from one to six. The percentage of phenotypic variance explained by each QTL ranged from 5.1% to 73.7%. For those traits for which two or more QTLs were detected, increases in the traits were conditioned by indica alleles at some QTLs Japonica alleles at others. No significant evidence was found for epistasis between markers associated with QTLs and all the other markers. Pleitropic effects of single QTLs on different traits are suggested by the observation of clustering of QTLs. No QTL for traits was found to map to the vicinity of major gene loci governing the same traits qualitatively. Evidence for putative orthologous QTLs across rice, maize, oat, and barley is discussed.
An E. coli Ribonucleoprotein Containing 4.5S RNA Resembles Mammalian Signal Recognition Particle

The signal recognition particle (SRP) plays a central role in directing the export of nascent proteins from the cytoplasm of mammalian cells. An SRP-dependent translocation machinery in bacteria has not been demonstrated in previous genetic and biochemical studies. Sequence comparisons, however, have identified (i) a gene in Escherichia coli (ffh) whose product is homologous to the 54-kilodalton subunit (SRP54) of SRP, and (ii) an RNA encoded by the ffs gene (4.5S RNA) that shares a conserved domain with the 7SL RNA of SRP. An antiserum to Ffh precipitated 4.5S RNA from E. coli extracts, implying that the two molecules reside in a complex. The 4.5S RNA can also bind to SRP54 and can replace 7SL RNA in an enzymatic assay. The product of a dominant mutation in the ffs gene (4.5S RNAdl1) is also coprecipitated by the antiserum to Ffh protein and is lethal when expressed from an inducible promoter. After induction of 4.5S RNAdl1, the earliest observed phenotype was a permanent induction of the heat shock response, suggesting that there was an accumulation of aberrant proteins in the cytoplasm. Late after induction, translocation of beta-lactamase was impaired; this may be an indirect effect of heat shock, however, because translocation of ribose binding protein or of the porin, OmpA, was unaffected. An unusual separation of the inner and outer membranes, suggestive of a defect in cell envelope, was also observed. Protein synthesis did not cease until very late, an indication that 4.5S RNA probably does not have a direct role in this process.
Reduced liver fibrosis in hypoxia-inducible factor-1α-deficient mice

Liver fibrosis is characterized by excessive deposition of extracellular matrix in the liver during chronic injury. During early stages of this disease, cells begin to synthesize and secrete profibrotic proteins that stimulate matrix production and inhibit matrix degradation. Although it is clear that these proteins are important for development of fibrosis, what remains unknown is the mechanism by which chronic liver injury stimulates their production. In the present study, the hypothesis was tested that hypoxia-inducible factor-1α (HIF-1α) is activated in the liver during chronic injury and regulates expression of profibrotic proteins. To investigate this hypothesis, mice were subjected to bile duct ligation (BDL), an animal model of liver fibrosis. HIF-1α protein was increased in the livers of mice subjected to BDL by 3 days after surgery. To test the hypothesis that HIF-1α is required for the development of fibrosis, control and HIF-1α-deficient mice were subjected to BDL. Levels of type I collagen and α-smooth muscle actin mRNA and protein were increased in control mice by 14 days after BDL. These levels were significantly reduced in HIF-1α-deficient mice. Next, the levels of several profibrotic mediators were measured to elucidate the mechanism by which HIF-1α promotes liver fibrosis. Platelet-derived growth factor (PDGF)-A, PDGF-B, and plasminogen activator inhibitor-1 mRNA levels were increased to a greater extent in control mice subjected to BDL compared with HIF-1α-deficient mice at 7 and 14 days after BDL. Results from these studies suggest that HIF-1α is a critical regulator of profibrotic mediator production during the development of liver fibrosis.
Structural basis of HIV-1 Vpu-mediated BST2 antagonism via hijacking of the clathrin adaptor protein complex 1

BST2/tetherin, an antiviral restriction factor, inhibits the release of enveloped viruses from the cell surface. Human immunodeficiency virus-1 (HIV-1) antagonizes BST2 through viral protein u (Vpu), which downregulates BST2 from the cell surface. We report the crystal structure of a protein complex containing Vpu and BST2 cytoplasmic domains and the core of the clathrin adaptor protein complex 1 (AP1). This, together with our biochemical and functional validations, reveals how Vpu hijacks the AP1-dependent membrane trafficking pathways to mistraffick BST2. Vpu mimics a canonical acidic dileucine-sorting motif to bind AP1 in the cytosol, while simultaneously interacting with BST2 in the membrane. These interactions enable Vpu to build on an intrinsic interaction between BST2 and AP1, presumably causing the observed retention of BST2 in juxtanuclear endosomes and stimulating its degradation in lysosomes. The ability of Vpu to hijack AP-dependent trafficking pathways suggests a potential common theme for Vpu-mediated downregulation of host proteins. DOI: http://dx.doi.org/10.7554/eLife.02362.001
Provider readiness for neonatal resuscitation in rural hospitals.

ABSTRACT Objective To describe nurse and physician readiness for neonatal resuscitation in rural hospitals. Design Descriptive, correlational, and comparative. Setting Twenty-six rural hospitals in two Midwestern states. Participants Rural providers of care to newborns (165 nurses and 59 physicians). Instruments Neonatal Resuscitation Index (knowledge about neonatal resuscitation) and Neonatal Resuscitation Experience Index (comfort and recent skill performance). Results The average Neonatal Resuscitation Index (knowledge) score of 69% was low. Many skills needed for full resuscitation had not been performed by rural providers during the previous year. Nurses reported lower levels of comfort with skills needed for full resuscitation ( t =−4.68; p r =.50 vs. .34). Nurses who were current Neonatal Resuscitation Program providers had significantly higher average levels of comfort (3.67 vs. 3.11; p p p Conclusions Maintaining high levels of readiness for neonatal resuscitation in rural hospitals is challenging. Nurses and physicians should make special effort to obtain continuing neonatal resuscitation education to ensure optimal outcomes when newborn emergencies arise in rural hospitals. Teamwork training for neonatal resuscitation readiness is an important topic for future rural health research.
Sources of variability in the flavonoid content of foods

Abstract In 2012, USDA prepared Release 3.1 of the “USDA Database for the Flavonoid Content of Selected Foods”, which contained data on 508 food items collected from 308 published sources. Flavonoids are secondary metabolites produced by plants in response to various environmental stresses such as climate and ultraviolet radiation. Other sources of variability include cultivar, growing location, agricultural practices, processing techniques and preparation methods, as well as analytical variability. The objective of this manuscript is to examine and report on variability in the flavonoid content of foods. While the required information needed to assess variability is not available for all foods, data for a number of foods was analyzed using analysis of variance for cultivar, location and other factors. For orange juice, data for 247 samples representing 109 mean values, i.e., different cultivars and location, were analyzed. The overall range for hesperetin was 1-39 mg/100 g; samples from the United States 5-30 mg/100 g; samples from Brazil 5-25 mg/100 g; and samples from Corsica (France) 12-26 mg/100 g. For strawberries, data for 148 samples representing 98 mean values were analyzed. The overall range for pelargonidin was 8-58 mg/100 g. The range of quercetin values in raw yellow onions is 0-91 mg/100 g, and represents 96 mean values for 402 samples from the U.S., Japan, Spain, and other countries. The flavonoid content of foods is extremely variable and is influenced by both location and cultivar, which account for 25 to 33 percent of the variability as well as by numerous other factors, which were not examined in this analysis.
ORTHOPEDIC ASPECTS OF CHILD ABUSE

Fractures are one of the most common manifestations of child abuse. Pediatricians and other physicians caring for children must have a clear understanding of the injury patterns sustained by children in abusive situations and be able to recognize those injuries. A thorough history and physical examination and accurate radiographic interpretation are essential. Early identification of nonaccidental trauma is imperative because proper intervention has been shown to be effective. Language: en
Basement and Regional Structure Along Strike of the Queen Charlotte Fault in the Context of Modern and Historical Earthquake Ruptures

The Queen Charlotte fault (QCF) is a dextral transform system located offshore of southeastern Alaska and western Canada, accommodating ∼4.4 cm/yr of relative motion between the Pacific and North American plates. Oblique convergence along the fault increases southward, and how this convergence is accommodated is still debated. Using seismic reflection data, we interpret offshore basement structure, faulting, and stratigraphy to provide a geological context for two recent earthquakes, an M w 7.5 strike‐slip event near Craig, Alaska, and an M w 7.8 thrust event near Haida Gwaii, Canada. We map downwarped Pacific oceanic crust near 54° N, between the two rupture zones. Observed downwarping decreases north and south of 54° N, parallel to the strike of the QCF. Bending of the Pacific plate here may have initiated with increased convergence rates due to a plate motion change at ∼6 Ma. Tectonic reconstruction implies convergence‐driven Pacific plate flexure, beginning at 6 Ma south of a 10° bend the QCF (which is currently at 53.2° N) and lasting until the plate translated past the bend by ∼2 Ma. Normal‐faulted approximately late Miocene sediment above the deep flexural depression at 54° N, topped by relatively undeformed Pleistocene and younger sediment, supports this model. Aftershocks of the Haida Gwaii event indicate a normal‐faulting stress regime, suggesting present‐day plate flexure and underthrusting, which is also consistent with reconstruction of past conditions. We thus favor a Pacific plate underthrusting model to initiate flexure and accommodation space for sediment loading. In addition, mapped structures indicate two possible fault segment boundaries along the QCF at 53.2° N and at 56° N.
Characterization of microsatellite loci for five members of the minnow family Cyprinidae found in the Sacramento-San Joaquin Delta and its tributaries

Two microsatellite-enriched libraries [(CAGA) n , (TAGA) n ] were constructed using pooled DNA from three cyprinid species native to the Sacramento–San Joaquin Delta of California: Sacramento splittail ( Pogonichthys macrolepidotus ); Sacramento pikeminnow ( Ptychocheilus grandis ); and tui chub ( Siphateles bicolor ). Primers were designed for 105 loci and tested for levels of polymorphism in five cyprinid species found in the Delta: Sacramento splittail, Sacramento pikeminnow, tui chub, hitch ( Lavinia exilicauda ), and Sacramento blackfish ( Orthodon microlepidotus ). Fifty-one loci were polymorphic for at least one species and 31 loci were polymorphic for multiple species. The number of polymorphic loci per species ranged from 16 to 26.
Neuronal properties and trophic activities of immortalized hippocampal cells from embryonic and young adult mice

The hippocampal formation elaborates trophic factors such as nerve growth factor (NGF) to support the cholinergic innervation it receives from the septal region. To further study the trophic interactions of this pathway, hippocampal cells from embryonic day 18 and postnatal day 21 mice were immortalized via somatic cell fusion to N18TG2 neuroblastoma cells. The hippocampal cell lines exhibit morphological and cytoskeletal features which are typical of their neuronal parents but which are not expressed by the neuroblastoma parent. When differentiated with retinoic acid, the hippocampal cell lines exhibit electrophysiological features similar to cultured hippocampal neurons. Many of the lines constitutively express high levels of NGF, and at least one cell line exerts a non-NGF trophic effect on the expression of choline acetyltransferase by septal neurons in vitro. These cell lines are potentially useful for investigating the neurochemical and excitable properties of hippocampal neurons and identifying novel trophic activities that promote the development and maintenance of the septohippocampal pathway.
Reducibility and eigenvalue sensitivity for identifying critical generators in multimachine power systems

A selection procedure to identify critical generators to be equipped with power system stabilizers is proposed. The selection is based on the sensitivity of critical eigenvalues to a parameter of the state coefficient matrix. Critical generators are identified by observing the sensitivities of the eigenvalues with respect to parameters which affect the damping of the machine rotor oscillation. These parameters are the diagonal elements of the coefficient matrix. Unlike other methods, the proposed method for calculating eigenvalue sensitivity does not require any eigenvectors. There are also no derivatives involved. One of the main advantages of this method is that it shows the dependence of eigenvalue sensitivity on power system structure; this may be helpful in reducing the size of the system. The effectiveness of the method is described through the application to a multi-machine power system dynamic model. >
Wireless technologies in sleep monitoring

Sleep disorders affect over 40 million patients and represent a significant a significant risk factor for many, often life threatening diseases. Technologies available for diagnostics and treatment typically involve the recording of key biomedical signals including ECG, EEG, EMG, SpO 2 , and respiration. Overnight measurement of such parameters is often compromised by the discomfort imposed by conventional measurement methods, or interference or sensor failure resulting from patient motion. This paper will provide an overview of the measurement of key sleep-related biomedical signatures which can be obtained without wiring or even contact physical contact with the patient.
The stochastic lake game: A numerical solution

Abstract In this paper, we numerically solve a stochastic dynamic programming problem for the solution of a stochastic dynamic game for which there is a potential function. The players select a mean level of control. The state transition dynamics is a function of the current state of the system and a multiplicative noise factor on the control variables of the players. The particular application is for lake water usage. The control variables are the levels of phosphorus discharged (typically by farmers) into the watershed of the lake, and the random shock is the rainfall that washes the phosphorus into the lake. The state of the system is the accumulated level of phosphorus in the lake. The system dynamics are sufficiently nonlinear so that there can be two Nash equilibria. A Skiba-like point can be present in the optimal control solution. We analyze (numerically) how the dynamics and the Skiba-like point change as the variance of the noise (the rain) increases. The numerical analysis uses a result of Dechert (1978. Optimal control problems from second order difference equations. Journal of Economic Theory 19, 50–63) to construct a potential function for the dynamic game. This greatly reduces the computational burden in finding Nash equilibria solutions for the dynamic game.
Characterization and genetic mapping of simple sequence repeats in the rice genome.

We searched partial sequences of over 22,706 rice cDNA and 1220 genomic DNA clones to find and characterize simple sequence repeats (SSRs) in the rice genome. The most frequently found repeated SSR motif in both cDNA and genomic DNA sequences was d(CCG/CGG)n. The second most frequently found SSR was d(AG/CT)n. In contrast with mammalian genomes, in which d(AC/GT)n sequences are the most abundant, d(AC/GT)n sequences were not frequently observed in rice. Sequences containing d(CCG/CGG)n, d(AG/CT)n repeats, and other SSRs were chosen for polymorphism detection. It was predicted that 17 of 20 SSRs in cDNA sequences were located in 5'-untranslated regions near initiation codons. Twenty-two loci can be mapped on our RFLP linkage map by these SSRs. Six markers were tested with 16 japonica rice varieties as templates for PCR. Two markers exhibited amplified fragment length polymorphism among these rice varieties, implying that SSRs are polymorphic among rice varieties which have similar genetic backgrounds. Even these polymorphic SSRs are located within or around genes which code ubiquitous proteins.
Host DNA Degradation after Infection of Escherichia coli with Bacteriophage T4: Dependence of the Alternate Pathway of Degradation Which Occurs in the Absence of Both T4 Endonuclease II and Nuclear Disruption on T4 Endonuclease IV

Abstract Escherichia coli cells infected with T4 phage which are deficient in both nuclear disruption and endonuclease II exhibit a pathway of host DNA degradation which does not occur in cells infected with phage deficient only in endonuclease II. This alternate pathway of host DNA degradation requires T4 endonuclease IV.
Variation of hamstrings lengths and velocities with walking speed

Crouch gait, one of the most prevalent movement abnormalities among children with cerebral palsy, is frequently treated with surgical lengthening of the hamstrings. To assist in surgical planning many clinical centers use musculoskeletal modeling to help determine if a patient’s hamstrings are shorter or lengthen more slowly than during unimpaired gait. However, some subjects with crouch gait walk slowly, and gait speed may affect peak hamstring lengths and lengthening velocities. The purpose of this study was to evaluate the effects of walking speed on hamstrings lengths and velocities in a group of unimpaired subjects over a large range of speeds and to determine if evaluating subjects with crouch gait using speed matched controls alters subjects’ characterization as having ‘‘short’’ or ‘‘slow’’ hamstrings. We examined 39 unimpaired subjects who walked at five different speeds. These subjects served as speed-matched controls for comparison to 74 subjects with cerebral palsy who walked in crouch gait. Our analysis revealed that peak hamstrings length and peak lengthening velocity in unimpaired subjects increased significantly with increasing walking speed. Fewer subjects with cerebral palsy were categorized as having hamstrings that were ‘‘short’’ (31/74) or ‘‘slow’’ (38/74) using a speed-matched control protocol compared to a non-speed-matched protocol (35/74 ‘‘short’’, 47/74 ‘‘slow’’). Evaluation of patients with cerebral palsy using speed-matched controls alters and may improve selection of patients for hamstrings lengthening procedures.
Vertically-stacked on-chip SiGe/BiCMOS/RFCMOS coplanar waveguides

This paper presents a new on-chip transmission line interconnect structure which offers the potential of superior return and insertion loss characteristics compared to the equivalent standard transmission line device. Conventional on-chip coplanar waveguides (CPW) and differential pairs are routed in a single metal layer in the chip's metal-dielectric stack. The vertically stacked coplanar waveguide (PW) transmission lines presented here consist of metal lines on multiple metal levels connected by continuous via bars. The additional cross-sectional area of the VCPW topology decreases interconnect resistance while the increased effective device thickness increases capacitance to neighboring ground return lines leading to a characteristics impedance reduction.