cssi/SciSciGPT-SciSciNet huggingface dataset:
Epstein-Barr Virus dUTPase Induces Neuroinflammatory Mediators: Implications for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome.

Abstract Purpose Neuroinflammation is a common feature in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), affecting 85%–90% of all patients, yet the underlying mechanism or mechanisms responsible for the initiation and/or promotion of this process is largely unknown. Multiple reports, however, have suggested a role for Epstein-Barr virus (EBV), in particular, in ME/CFS, but its potential role, if any, in the neuroinflammatory process has not been addressed. In support of this premise, studies by our group have found that the EBV protein deoxyuridine triphosphate nucleotidohydrolase (dUTPase) induces anxiety and sickness behaviors in female mice. We also found that a small subset of patients with ME/CFS exhibited prolonged and significantly elevated neutralizing antibodies against EBV dUTPase protein in serum, which inversely correlated with ME/CFS symptoms. A larger ME/CFS case–control cohort study further confirmed that a significant percentage of patients with ME/CFS (30.91%–52.7%) were simultaneously producing antibodies against multiple human herpesviruses-encoded dUTPases and/or human dUTPase. Altogether, these findings suggest that EBV dUTPase protein may be involved in the neuroinflammatory process observed in ME/CFS. Thus, the aim of the present study was to determine whether the EBV dUTPase protein could contribute to neuroinflammation by altering the expression of genes involved with maintaining blood–brain barrier (BBB) integrity and/or modulating synaptic plasticity. Methods With the use of human immortalized astrocytes, microglia, and cerebral microvascular endothelial cells, we conducted time-course (0–24 h) experiments with EBV dUTPase protein (10 μg/mL) to determine what effect(s) it may have on the expression of genes involved with BBB permeability, astrocytes and microglia cell function, tryptophan metabolism, and synaptic plasticity by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In parallel, in vivo studies were conducted in female C57Bl/6 mice. Mice were injected by the intraperitoneal route with EBV dUTPase protein (10 μg) or vehicle daily for 5 days, and the brains were collected and processed for further qRT-PCR analysis of the in vivo effect of the dUTPase on the dopamine/serotonin and γ-aminobutyric acid/glutamate pathways, which are important for brain function, using RT2 Profiler PCR Arrays. Findings EBV dUTPase protein altered the expression in vitro (12 of 15 genes and 32 of 1000 proteins examined) and in vivo (34 of 84 genes examined) of targets with central roles in BBB integrity/function, fatigue, pain synapse structure, and function, as well as tryptophan, dopamine, and serotonin metabolism. Implications The data suggest that in a subset of patients with ME/CFS, the EBV dUTPase could initiate a neuroinflammatory reaction, which contributes to the fatigue, excessive pain, and cognitive impairments observed in these patients.
Solitude et souffrance des figures d’exception chez Kierkegaard. Sur la distance, la dissimulation et le silence face à Dieu

Dans cet article, nous relions la solitude a la souffrance chez Kierkegaard. Nous montrons que la pensee existentielle est un travail de distance, precisement un jeu de dissimulation dont la signification reside dans la capacite a sauvegarder le silence. Le secret de interiorite est intraduisible dans l’exteriorite du langage, ce qui isole et fait souffrir, mais ouvre le passage vers le religieux. Dieu seul peut voir les efforts de l’existant dissimulant dialectiquement sa souffrance dans la difference entre l’infini et le fini. Pour illustrer cette these, nous approfondissons les cas ou Kierkegaard presente la souffrance en recourant a des figures d’exception — des marqueurs de solitude — comme Abraham, Job et le quidam. Nous relisons l’« Histoire de la souffrance » des Stades et le Post-scriptum pour mieux saisir les liens entre la souffrance et la solitude — une idee qui se trouve justifiee dans le Point de vue explicatif — de celui qui se veut, finalement, seul devant Dieu.
Revisiting the Probability Distribution of Low Streamflow Series in the United States

AbstractDroughts result in billions of dollars in annual losses, loss of life, and the displacement of people. The characterization of hydrologic drought enhances the prediction of streamflow stati...
Health Benefits In 2019: Premiums Inch Higher, Employers Respond To Federal Policy

The annual Kaiser Family Foundation Employer Health Benefits Survey found that in 2019 the average annual premium for single coverage rose 4 percent to $7,188, and the average annual premium for fa...
Research on the Pathophysiology of Chiari I-Related Symptoms and Syringomyelia, with Emphasis on Dynamic MRI Techniques

The pathophysiology of Chiari-related symptoms and syringomyelia remains enigmatic. Present-day technology, most notably MR imaging, is providing progressively more sophisticated opportunities to test traditional as well as contemporary theories while adding to conventional animal models a selection of promising mechanical and computational representations of syringomyelia and its etiologies. We will briefly touch on the past and present of Chiari-related syringomyelia research with emphasis on essential questions related to the development of not just the anatomical anomalies but also the constellation of symptoms with which they are associated.
Pulmonary Vascular Diseases

This chapter describes pulmonary embolism and infarction, non-thrombotic pulmonary emboli (fat embolism syndrome, amniotic fluid emboli, foreign body emboli, tumor emboli), chronic pulmonary embolism, pulmonary arterial hypertension, and pulmonary veno-occlusive disease.
Associations between milk fat content and obesity, 1999 to 2016.

BACKGROUND The relationship between milk fat content and body mass index (BMI) remains unclear. The objective of this study was to determine if milk fat content is associated with obesity in children, adolescents and young adults. METHODS We used repeated cross sections of National Health and Nutrition Examination Survey (NHANES), 1999 to 2016. Using logistic regression, we measured associations between first milk consumed after formula/breast milk (whole, 2%, 1%, or fat-free) and weight status (≥85th to <95th BMI percentile, overweight; ≥95th BMI percentile, obesity) among children 2 to 6 years (n = 8367). We also assessed associations between current milk type and weight status among those 2 to 20 years of age (n = 26 750). RESULTS Children 2 to 6 years with obesity were less likely to have been weaned to whole milk versus healthy weight children (adjusted odds ratio [aOR], 0.77; 95% CI, 0.60-0.98; P = .031). Individuals 2 to 20 years with overweight or obesity were less likely to drink whole milk as their current milk and more likely to drink fat-free or 1% milk compared with healthy weight children. CONCLUSIONS Whole milk consumers are less likely to have obesity. It is unclear whether this relationship is causal or a reflection of United States Department of Agriculture recommendations. Further examination of the factors that influence milk choice among diverse cohorts would lend clarity to this important issue.
Targeting Fatty Acid Oxidation to Promote Anoikis and Inhibit Ovarian Cancer Progression

Epithelial-derived high-grade serous ovarian cancer (HGSOC) is the deadliest gynecologic malignancy. Roughly 80% of patients are diagnosed with late-stage disease, which is defined by wide-spread cancer dissemination throughout the pelvic and peritoneal cavities. HGSOC dissemination is dependent on tumor cells acquiring the ability to resist anoikis (apoptosis triggered by cell detachment). Epithelial cell detachment from the underlying basement membrane or extracellular matrix leads to cellular stress, including nutrient deprivation. In this report, we examined the contribution of fatty acid oxidation (FAO) in supporting anoikis resistance. We examined expression Carnitine Palmitoyltransferase 1A (CPT1A) in a panel of HGSOC cell lines cultured in adherent and suspension conditions. With CPT1A knockdown cells, we evaluated anoikis by caspase 3/7 activity, cleaved caspase 3 immunofluorescence, flow cytometry, and colony formation. We assessed CPT1A-dependent mitochondrial activity and tested the effect of exogenous oleic acid on anoikis and mitochondrial activity. In a patient-derived xenograft model, we administered etomoxir, an FAO inhibitor, and/or platinum-based chemotherapy. CPT1A is overexpressed in HGSOC, correlates with poor overall survival, and is upregulated in HGSOC cells cultured in suspension. CPT1A knockdown promoted anoikis and reduced viability of cells cultured in suspension. HGSOC cells in suspension culture are dependent on CPT1A for mitochondrial activity. In a patient-derived xenograft model of HGSOC, etomoxir significantly inhibited tumor progression. IMPLICATIONS: Targeting FAO in HGSOC to promote anoikis and attenuate dissemination is a potential approach to promote a more durable antitumor response and improve patient outcomes.
Acromelic dysplasias: how rare musculoskeletal disorders reveal biological functions of extracellular matrix proteins

Acromelic dysplasias are a group of rare musculoskeletal disorders that collectively present with short stature, pseudomuscular build, stiff joints, and tight skin. Acromelic dysplasias are caused by mutations in genes (FBN1, ADAMTSL2, ADAMTS10, ADAMTS17, LTBP2, and LTBP3) that encode secreted extracellular matrix proteins, and in SMAD4, an intracellular coregulator of transforming growth factor-β (TGF-β) signaling. The shared musculoskeletal presentations in acromelic dysplasias suggest that these proteins cooperate in a biological pathway, but also fulfill distinct roles in specific tissues that are affected in individual disorders of the acromelic dysplasia group. In addition, most of the affected proteins directly interact with fibrillin microfibrils in the extracellular matrix and have been linked to the regulation of TGF-β signaling. Together with recently developed knockout mouse models targeting the affected genes, novel insights into molecular mechanisms of how these proteins regulate musculoskeletal development and homeostasis have emerged. Here, we summarize the current knowledge highlighting pathogenic mechanisms of the different disorders that compose acromelic dysplasias and provide an overview of the emerging biological roles of the individual proteins that are compromised. Finally, we develop a conceptual model of how these proteins may interact and form an "acromelic dysplasia complex" on fibrillin microfibrils in connective tissues of the musculoskeletal system.
Space-Time Adaptive Detection at Low Sample Support

An important problem in space-time adaptive detection is the estimation of the large $p\times p$ interference covariance matrix from training signals. When the number of training signals $n$ is greater than $2p$ , existing estimators are generally considered to be adequate, as demonstrated by fixed-dimensional asymptotics. But in the low-sample-support regime ( $n or even $n ), fixed-dimensional asymptotics are no longer applicable. The remedy undertaken in this paper is to consider the “large dimensional limit” in which $n$ and $p$ go to infinity together. In this asymptotic regime, a new type of estimator is defined (Definition 2 ), shown to exist (Theorem 1), and shown to be detection-theoretically ideal (Theorem 2). Further, asymptotic conditional detection and false-alarm rates of filters formed from this type of estimator are characterized (Theorems 3 and 4) and shown to depend only on data that is given, even for non-Gaussian interference statistics. The paper concludes with several Monte Carlo simulations that compare the performance of the estimator in Theorem 1 to the predictions of Theorems 2-4, showing in particular higher detection probability than Steiner and Gerlach's Fast Maximum Likelihood estimator.
Feeding inequality: food insecurity, social status and college student health.

A growing but limited body of research has identified the college student population as one that is particularly vulnerable to food insecurity. Early estimates of food insecurity prevalence among college students range from 14 to 60 per cent. The present study utilises original survey data collected from a random sample (n = 300) of college students enrolled at an urban university in the Midwest region of the United States of America (USA). This study examines the impact of food insecurity on health outcomes and the mediation of this relationship by subjective social status among college students. Ordinary least squares (OLS) and logistic regression analyses find that food insecurity is related to worse self-rated, physical and mental health among college students, and Sobel-Goodman tests find that subjective social status plays a significant mediating role in the relationship between food insecurity and health among college students. The implications of these findings in a university context are discussed using a psychosocial framework and insights from the stress process model. In doing so, I discuss food insecurity among college students with an emphasis on the social significance of food and food insecurity.
Transcatheter aortic valve replacement after chest radiation: A propensity-matched analysis

Abstract Background Chest radiation therapy (CRT) for malignant thoracic neoplasms is associated with development of valvular heart disease years later. As previous radiation exposure can complicate surgical treatment, transcatheter aortic valve replacement (TAVR) has emerged as an alternative. However, outcomes data are lacking for TAVR patients with a history of CRT. Methods We conducted a retrospective study of all patients who underwent a TAVR procedure at a single institution between September 2012 and November 2018. Among 1341 total patients, 50 had previous CRT. These were propensity-matched in a 1:2 ratio to 100 patients without history of CRT. Thirty-day adverse events were analyzed with generalized estimating equation models. Overall mortality was analyzed with stratified Cox regression modelling. Results Median clinical follow-up was 24 months (interquartile range [IQR], 12–44 months). There was no difference between CRT and non-CRT patients in overall mortality (hazard ratio [HR] 0.84 [0.37–1.90], P = 0.67), 30-day mortality (HR 3.1 [0.49–20.03], P = 0.23), or 30-day readmission rate (HR 1.0 [0.43–2.31], P = 1). There were no differences in the rates of most adverse events, but patients with CRT history had higher rates of postprocedural respiratory failure (HR 3.63 [1.32–10.02], P = 0.01) and permanent pacemaker implantation (HR 2.84 [1.15–7.01], P = 0.02). Conclusions For patients with aortic valve stenosis and previous CRT, TAVR is safe and effective, with outcomes similar to those in the general aortic stenosis population. Patients with history of CRT are more likely to have postprocedural respiratory failure and to require permanent pacemaker implantation.
When what routinely happens conflicts with what ought to be done: a scenario-based assessment of secondary mathematics teachers’ decisions

This study analyses an online scenario-based instrument in which secondary mathematics teachers from across the United States were presented with episodes of mathematics instruction and then asked ...
Lifelong single-cell profiling of cranial neural crest diversification

The cranial neural crest generates a huge diversity of derivatives, including the bulk of connective and skeletal tissues of the vertebrate head. How neural crest cells acquire such extraordinary lineage potential remains unresolved. By integrating single-cell transcriptome and chromatin accessibility profiles of cranial neural crest-derived cells across the zebrafish lifetime, we observe region-specific establishment of enhancer accessibility for distinct fates. Neural crest-derived cells rapidly diversify into specialized progenitors, including multipotent skeletal progenitors, stromal cells with a regenerative signature, fibroblasts with a unique metabolic signature linked to skeletal integrity, and gill-specific progenitors generating cell types for respiration. By retrogradely mapping the emergence of lineage-specific chromatin accessibility, we identify a wealth of candidate lineage-priming factors, including a Gata3 regulatory circuit for respiratory cell fates. Rather than multilineage potential being an intrinsic property of cranial neural crest, our findings support progressive and region-specific chromatin remodeling underlying acquisition of diverse neural crest lineage potential. HighlightsO_LISingle-cell transcriptome and chromatin atlas of cranial neural crest C_LIO_LIProgressive emergence of region-specific cell fate competency C_LIO_LIChromatin accessibility mapping identifies candidate lineage regulators C_LIO_LIGata3 function linked to gill-specific respiratory program C_LI Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=173 SRC="FIGDIR/small/456710v1_ufig1.gif" ALT="Figure 1"> View larger version (69K): org.highwire.dtl.DTLVardef@1c4ca91org.highwire.dtl.DTLVardef@cf0d10org.highwire.dtl.DTLVardef@162bfe9org.highwire.dtl.DTLVardef@2565fd_HPS_FORMAT_FIGEXP M_FIG C_FIG
Energy Metabolism and the Induction of the Unfolded Protein Response: A Dissertation

White adipose plays a major role in the regulation of whole body metabolism through the storage and hydrolysis of triglycerides and by secretion of adipokines. The function of endocrine cells is highly dependent on the unfolded protein response (UPR), a homeostatic signaling mechanism that balances the protein folding capacity of the endoplasmic reticulum (ER) with the cell's secretory protein load. Here we demonstrate that the adipocyte UPR pathway is necessary for its secretory functions, and can thus play a crucial role in the control of whole body energy homeostasis. ER protein folding capacity is dependent both on the number of available chaperones as well as on their activity, which requires a sufficient ATP supply. In 3T3-L1 adipocytes, mitochondrial biogenesis occurred in parallel with induction of the UPR; therefore, we tested whether it was necessary for efficient ER function. Inhibition of mitochondrial ATP synthesis through depletion of Tfam, a mitochondrial transcription factor, or treatment with inhibitors of oxidative phosphorylation, demonstrate that ER function is sensitive to acute changes in adenine nucleotide levels. In addition, adenylate kinase 2 (AK2), which regulates mitochondrial adenine nucleotide interconversion, is markedly induced during adipocyte and B cell differentiation. AK2 depletion impairs induction of the UPR and secretion in both cell types. Interestingly, cytosolic adenylate kinase 1 (AK1) does not have the same effect upon UPR induction. We show that adenine nucleotides promote proper ER function and alterations in specific aspects of ATP synthesis can impair UPR signaling. Understanding the complex energetic regulation of the UPR may provide insight into the relationship between UPR and disease.
Vadose Zone Flow and Transport of Dissolved Organic Carbon at Multiple Scales in Humid Regimes

Scientists must embrace the necessity to offset global CO2 emissions regardless of politics. Efforts to enhance terrestrial organic carbon sequestration have traditionally focused on aboveground biomass and surface soils. An unexplored potential exists in thick lower horizons of widespread, mature soils such as Alfisols, Ultisols, and Oxisols. We present a case study of fate and transport of dissolved organic carbon (DOC) in a highly weathered Ultisol, involving spatial scales from the laboratory to the landscape. Our objectives were to interpret processes observed at various scales and provide an improved understanding of coupled hydrogeochemical mechanisms that control DOC mobility and sequestration in deep subsoils within humid climatic regimes. Our approach is multiscale, using laboratory-scale batch and soil columns (0.2 by 1.0 m), an in situ pedon (2 by 2 by 3 m), a well-instrumented subsurface facility on a subwatershed (0.47 ha), and ephemeral and perennial stream discharge at the landscape scale (38.4 ha). Laboratoryscale experiments confirmed that lower horizons have the propensity to accumulate DOC, but that preferential fracture flow tends to limit sequestration. Intermediate-scale experiments demonstrated the beneficial effects of C diffusion into soil micropores. Field- and landscapescale studies demonstrated coupled hydrological, geochemical, and microbiological mechanisms that limit DOC sequestration, and their sensitivity to local environmental conditions. Our results suggest a multi-scale approach is necessary to assess the propensity of deep subsoils to sequester organic C in situ. By unraveling fundamental organic C sequestration mechanisms, we improve the conceptual and quantitative understanding needed to predict and alter organic C budgets in soil systems.
The Heat Budget of Lakes

One of the most important and interesting characteristics of a lake is its thermal structure. The heat content of a body of water is of vital importance in limnology. The metabolism, physiology, and behavior of aquatic organisms are related directly to the temperature of the aquatic environment. Extreme temperatures restrict the growth and distribution of plants, animals, and microbes. Because of the high specific heat of water, large volumes of water change temperature relatively slowly. Therefore, large lakes tend to moderate local climates, provide longer growing seasons for aquatic life, and serve as integrated recorders of recent climatic phenomena. For these and other reasons, the thermal structure and heat content of a body of water must be known with some degree of accuracy in limnological studies.
Glutamic acid 203 of the cAMP-dependent protein kinase catalytic subunit participates in the inhibition by two isoforms of the protein kinase inhibitor.

Although the protein kinase inhibitors (PKIs) are known to be potent and specific inhibitors of the catalytic (C) subunit of cAMP-dependent protein kinase, little is known about their physiological roles. Glutamate 203 of the C alpha isoform (C alpha E203) has been implicated in the binding of the arginine 15 residue of the skeletal isoform of PKI (PKI alpha R15) (Knighton, D. R., Zheng, J., Ten Eyck, L. F., Xuong, N., Taylor, S.S., and Sowadski, J. M. (1991) Science 253, 414-420). To investigate the role of C alpha E203 in the binding of PKI and in vivo C-PKI interactions, in vitro mutagenesis was used to change the C alpha E203 codon of the murine C alpha cDNA to alanine and glutamine codons. Initially, the C alpha E203 mutant proteins were expressed and purified from Escherichia coli. C alpha E203 is not essential for catalysis as all of the C subunit mutants were enzymatically active. The mutation of Glu203 did increase the apparent Km for Leu-Arg-Arg-Ala-Ser-Leu-Gly (Kemptide) severalfold but did not affect the apparent Km for ATP. The Vmax(app) was not affected by the mutation of C alpha E203. The mutation of C alpha E203 compromised the ability of PKI alpha (5-24), PKI alpha, and PKI beta to inhibit phosphotransferase activity. PKI alpha was altered using in vitro mutagenesis to probe the role of Arg15 in interacting with C alpha E203. The PKI alpha R15A mutant was reduced in its inhibition of C alpha. Preliminary studies of the expression of these C alpha mutants in COS cells gave similar results. These results suggest that the C alpha E203 mutants may be useful in assessing the role of PKI in vivo.
Exploring URL hit priors for web search

URL usually contains meaningful information for measuring the relevance of a Web page to a query in Web search. Some existing works utilize URL depth priors (i.e. the probability of being a good page given the length and depth of a URL) for improving some types of Web search tasks. This paper suggests the use of the location of query terms occur in a URL for measuring how well a web page is matched with a user's information need in web search. First, we define and estimate URL hit types, i.e. the priori probability of being a good answer given the type of query term hits in the URL. The main advantage of URL hit priors (over depth priors) is that it can achieve stable improvement for both informational and navigational queries. Second, an obstacle of exploiting such priors is that shortening and concatenation are frequently used in a URL. Our investigation shows that only 30% URL hits are recognized by an ordinary word breaking approach. Thus we combine three methods to improve matching. Finally, the priors are integrated into the probabilistic model for enhancing web document retrieval. Our experiments were conducted using 7 query sets of TREC2002, TREC2003 and TREC2004, and show that the proposed approach is stable and improve retrieval effectiveness by 4%~11% for navigational queries and 10% for informational queries.
Neural-Network-Based Parameter Estimation in S-System Models of Biological Networks

The genomic and post-genomic eras have been blessing us with overwhelming amounts of data that are of increasing quality. The challenge is that most of these data alone are mere snapshots of the functioning organism and do not reveal the organizational structure of which the particular genes and metabolites are contributors. To gain an appreciation of their roles and functions within cells and organisms, genomic and metabolic data need to be integrated in systems models that allow the testing of hypotheses, generate experimentally testable predictions, and ultimately lead to true explanations. One type of data that is particularly well suited for such integration consists of time profiles, which show gene activities, metabolite concentrations, or protein prevalences at dense series of time points. We show with a specific example how such time series can be analyzed and evaluated, if some structural information about the data is available, even if this information is incomplete. The method consists of three components. The first is a particularly suitable mathematical modeling framework, namely Biochemical Systems Theory, in which parameters are direct indicators of the organization of the underlying phenomenon, the second is the training of an artificial neural network for data smoothing and complementation, and the third is a technique for reinterpreting differential equations in a fashion that facilitates parameter estimation. A prototype webtool for these analyses is available at https://bioinformatics.musc.edu/webmetabol/.
Minimal subspace rotation on the Stiefel manifold for stabilization and enhancement of projection-based reduced order models for the compressible Navier-Stokes equations

For a projection-based reduced order model (ROM) of a fluid flow to be stable and accurate, the dynamics of the truncated subspace must be taken into account. This paper proposes an approach for stabilizing and enhancing projection-based fluid ROMs in which truncated modes are accounted for a priori via a minimal rotation of the projection subspace. Attention is focused on the full non-linear compressible Navier-Stokes equations in specific volume form as a step toward a more general formulation for problems with generic non-linearities. Unlike traditional approaches, no empirical turbulence modeling terms are required, and consistency between the ROM and the Navier-Stokes equation from which the ROM is derived is maintained. Mathematically, the approach is formulated as a trace minimization problem on the Stiefel manifold. The reproductive as well as predictive capabilities of the method are evaluated on several compressible flow problems, including a problem involving laminar flow over an airfoil with a high angle of attack, and a channel-driven cavity flow problem.
Including a Range of Outcome Targets Offers a Broader View of Fibromyalgia Treatment Outcome: Results from a Retrospective Review of Multidisciplinary Treatment

Background Fibromyalgia is associated with substantial functional disability. Current drug and non-drug treatments result in statistically significant but numerically small improvements in typical numeric measures of pain severity and fibromyalgia impact. Objective The aim of the present study was to evaluate additional measures of pain severity and functional outcome that might be affected by fibromyalgia treatment. Methods This retrospective review evaluated outcomes from 274 adults with fibromyalgia who participated in a six-week, multidisciplinary treatment programme. Pain and function were evaluated on the first and final treatment visit. Pain was evaluated using an 11-point numerical scale to determine clinically meaningful pain reduction (decrease ≥ 2 points) and from a pain drawing. Function was evaluated by measuring active range of motion (ROM), walking distance and speed, upper extremity exercise repetitions, and self-reports of daily activities. Results Numerical rating scores for pain decreased by 10–13% (p < 0.01) and Fibromyalgia Impact Questionnaire (FIQ) scores decreased by 20% (p < 0.001). More substantial improvements were noted when using alternative measures. Clinically meaningful pain relief was achieved by 37% of patients, and the body area affected by pain decreased by 31%. ROM showed significant improvements in straight leg raise and cervical motion, without improvements in lumbar ROM. Daily walking distance increased fourfold and arm exercise repetitions doubled. Conclusion Despite modest albeit statistically significant improvements in standard measures of pain severity and the FIQ, more substantial pain improvement was noted when utilizing alternative measures of pain and functional improvement. Alternative symptom assessment measures might be important outcome measures to include in drug and non-drug studies to better understand fibromyalgia treatment effectiveness. Copyright © 2013 John Wiley & Sons, Ltd.
Successful endovascular repair of two ruptured thoracic aortic aneurysms in nonagenarians

Background Ruptured thoracic aortic aneurysm (rTAA) is a catastrophic and mortal event. Thoracic endoVascular aortic repair (TEVAR) has emerged as an alternative to open repair. We report the first two successful TEVAR performed for rTAA in nonagenarians. Methods and Results Patient 1 was a 92-year-old man with multiple comorbidities with a 5.6 cm thoracic aortic aneurysm who was admitted for anticoagulation for pulmonary embolism. Twelve hours later, he was found to be hypotensive and the X-ray showed an opacified left hemithorax ( Fig. 1 ). A 40 mm × 20 cm Gore TAG stent-graft (W. L. Gore & Associates, Inc., Flagstaff, AZ) was deployed to successfully exclude the rupture. The postoperative course was uncomplicated and on day 9, he was discharged to a skilled nursing facility. Patient 2 was a 94-year-old man with a history of multiple comorbidites and endovascular aneurysm repair for ruptured abdominal aortic aneurysm 3 years earlier, who presented to the emergency room in hemorrhagic shock. Computed tomography scan revealed hemomediastinum and left hemothorax suggesting thoracic aorta rupture ( Fig. 2 A). Emergently, a 34 mm × 30 cm Gore TAG stent-graft was deployed ( Fig. 2 B). A left chest tube was placed. Postoperative course was briefly complicated by acute renal failure and pneumonia and on day 14, he was discharged to a rehabilitation center. Conclusion TEVAR for rTAA is an effective option and advanced age alone should not deter definitive repair of the thoracic aorta.
Thomas Hardy's sense of Self: The Poet behind the autobiographer in the life of Thomas Hardy

(1980). Thomas Hardy's sense of Self: The Poet behind the autobiographer in the life of Thomas Hardy. Prose Studies: Vol. 3, No. 1, pp. 69-86.
Analysis of Matched Samples

In order to study the association between disease status (eg, nosocomial infection) and some exposure variable (eg, number of days on urinary catheter), it is often necessary to take into account other variables that may influence either the disease status or the exposure variable. For example, Freeman et al described a retrospective (in their terminology "casereferent") study of a neonatal population.' In this study, cases of nosocomial infection are selected in addition to corresponding control individuals who did not experience a nosocomial infection. All patients were selected from a neonatal intensive care unit, and the goal was to study the role of umbilical artery catheterization and its association with nosocomial infection. As noted by Freeman et al, this is a complex question because the risk of nosocomial infection might reasonably depend not only on the duration of catheterization, but also on the birth weight of the infant.' There are a number of ways in which such a study might be conducted. One might select cases of nosocomial infection from this unit and also select corre-
Turkish Migration to the United States: From Ottoman Times to the Present

The article presents a review of the book "Turkish Migration to the United States: From Ottoman Times to the Present," edited by A. Deniz Balgamis and Kemal H. Karpat.
Empirical and hierarchical Bayes competitors of preliminary test estimators in two sample problems

Abstract We consider the problem of estimation of μ 1 when it is suspected that μ 1 ≈ μ 2 based on independent samples from N p (μ 1 , σ 2 V 1 ) and N p (μ 2 , σ 2 V 2 ). We assume V 1 , V 2 known but σ 2 unknown. First, the EB estimator is derived and its Bayesian and frequentist properties are studied. Second, a modified EB estimator is proposed and shown to dominate a preliminary test estimator. Finally, a hierarchical Bayes approach is proposed as an alternative to EB estimators.
Degradation of the subunits of receptor-bound human choriogonadotropin by Leydig tumor cells

Abstract Biologically active, iodine-labeled derivatives of human choriogonadotropin in which all the iodine is localized either in the α or β subunits have been prepared. It was found that upon binding to Leydig tumor cells these derivatives are ultimately degraded to 3′-monoidotyrosine. A comparison of the rates of degradation of the derivatives labeled exclusively in the α or β sununits show that the α subunit is degraded somewhat faster than the β subunit. It was also found that NH4Cl, chloroquine and leupeptin inhibited the degradation of both subunits to the same extent. peptin inhibited the degradation of both subunits to the same extent. These results show that the Leydig tumor cells degrade both subunits of the receptor-bound human choriogonadotropin, and suggest that the two subunits are degraded by the same mechanism(s).
Decadal Prediction in the Pacific Region

Abstract A “perfect model” configuration with a global coupled climate model 30-member ensemble is used to address decadal prediction of Pacific SSTs. All model data are low-pass filtered to focus on the low-frequency decadal component. The first three EOFs in the twentieth-century simulation, representing nearly 80% of the total variance, are used as the basis for early twenty-first-century predictions. The first two EOFs represent the forced trend and the interdecadal Pacific oscillation (IPO), respectively, as noted in previous studies, and the third has elements of both trend and IPO patterns. The perfect model reference simulation, the target for the prediction, is taken as the experiment that ran continuously from the twentieth to twenty-first century using anthropogenic and natural forcings for the twentieth century and the A1B scenario for the twenty-first century. The other 29 members use a perturbation in the atmosphere at year 2000 and are run until 2061. Since the IPO has been recognized as a ...
Why do black basketball players work more for less money

Abstract We explore Kahn and Sherer’s finding that National Basketball Association players’ salaries are lower for black than white players. In general, we do not find evidence that this salary differential is due to the racial preferences of fans. Specifically, we observe that black players actually play more than comparable white players. We offer a theory of price discrimination based on relative supplies and supply elasticities, in lieu of the racial discrimination argument, as an explanation for the black/white wage differential.
Optically pumped HBr gas laser operating in regions of high atmospheric transmission

An optically pumped overtone HBr laser is investigated experimentally and theoretically. The frequency tuning and stabilization of the Nd:YAG pump laser is described. Results of HBr laser emission are presented. The simulation shows promising features of both pulsed and cw pumped systems concerning efficiency, frequency tuning and heat dissipation.
Anti-genetic engineering activism and scientized politics in the case of “contaminated” Mexican maize

The struggle over genetically-engineered (GE) maize in Mexico reveals a deep conflict over the criteria used in the governance of agri-food systems. Policy debate on the topic of GE maize has become “scientized,” granting experts a high level of political authority, and narrowing the regulatory domain to matters that can be adjudicated on the basis of scientific information or “managed” by environmental experts. While scientization would seem to narrow opportunities for public participation, this study finds that Mexican activists acting “in defense of maize” engage science in multiple ways, using and producing scientific knowledge as well as treating scientific discussions as a stage for launching complex social critiques. Drawing from research in science and technology studies, this article assesses the impacts and pitfalls of three tactics used by maize activists that respond to the scientization of biotechnology politics: (1) using scientific information as a resource; (2) participating in scientific research; and (3) reframing policy problems as broadly social, rather than as solely scientific or technical. The obstacles that maize activists have faced in carrying out each of these efforts indicate that despite diverse and sophisticated engagements between social movements and the scientific field, scientization remains a significant institutional barrier to democratizing agricultural governance.
Facile Liquid Chromatographic Separation of Positional Isomers with a γ-Cyclodextrin Bonded Phase Column

Abstract Facile isocratic liquid chromatographic separation of positional isomers of substituted anilines is demonstrated using a γ-cyclodextrin (γ-CD) bonded phase column. It is found that normal phase separation is more effective than reversed-phase separation for these compounds. The effect of mobile phase composition on the retention of several substituted anilines is also examined. A minimum value of the capacity factor (k') for each compound is seen at a mobile phase composition of 60% 2-propanol in water and a rationalization of this unusual observation is proposed. The effect of cavity size of cyclodextrins on the retention of solutes is also discussed.
Mammalian palaeobiology: living large in the Cretaceous.

Discoveries of large, carnivorous mammals from the Cretaceous challenge the long-held view that primitive mammals were small and uninteresting. Have palaeontologists been asking the wrong questions?
The Attitudinal Structure of African American Women Party Activists: The Impact of Race, Gender, and Religion

Using data on grassroots party activists in the South, we explore the attitudinal structure of Democratic activists. Because of their socialization experiences with both racial and gender discrimination, we hypothesize that African American women will have a unique attitudinal structure regarding racial and gender issues when compared to other activist subgroups. Our results indicate the importance of race, gender, and the black church for understanding the structure of African American women’s political attitudes. Unlike whites or black men, African American women locate issues of race and gender on a single dimension; whether abortion attitudes fit on that dimension depends on the religious involvement of these activists.
Anticardiolipin Antibodies From Patients With the Antiphospholipid Antibody Syndrome Recognize Epitopes in Both β2-Glycoprotein 1 and Oxidized Low-Density Lipoprotein

Background—We recently suggested that many anticardiolipin antibodies bind only to oxidized cardiolipin (OxCL) and/or to OxCL–β2-glycoprotein 1 (β2GP1) adducts but not to a “reduced” cardiolipin that is unable to undergo oxidation. To test this hypothesis, we investigated 24 sera, 4 protein A–purified IgG fractions, and 3 human monoclonal antibodies that were all isolated from patients with antiphospholipid antibody syndrome (APS); testing was also performed in 7 controls. Two monoclonal antibodies (IS3 and IS4) were selected for binding to CL and one was selected for binding to β2GP1 (LJB8). Methods and Results—By chemiluminescent immunoassay, all APS sera samples bound only to OxCL and not to reduced CL, and the binding was inhibited >95% by OxCL but not reduced CL. All purified IgG fractions bound to β2GP1 but only when the β2GP1 was plated on microtiter wells coated with OxCL. All 3 monoclonal antibodies bound only to OxCL. On Western blots, IS4 and LJB8 bound to β2GP1 as well as to delipidated apoB o...
Oxidative Regeneration of Sulfide-fouled Catalysts for Water Treatment

This study tested the stability, activity, and selectivity of an alumina-supported Pd–In bimetallic catalyst during repetitive sulfide fouling and oxidative regeneration conditions. Nitrate reduction with hydrogen was used as the probe reaction in a continuous-flow packed-bed reactor to assess changes in the catalyst structure as a result of the fouling and regeneration processes. Partial regeneration of a severely sulfide-fouled Pd–In catalyst was achieved with a NaOCl/NaHCO3 solution. However, the regenerated catalyst had a reduced activity for NO3 − reduction and increased selectivity towards NH3. Analysis of the catalyst bed after regeneration experiments using XPS, ICP-MS, and BET surface area revealed that bulk structural transformations of the Pd–In bimetallic catalyst occurred, as a result of preferential Pd dissolution near the column influent. The dissolved Pd showed limited mobility in the column, and was re-deposited on the catalyst, resulting in Pd enrichment on the catalyst surface and redistribution of Pd towards the end of the column. These changes along with residual sulfur content on the catalyst surface were likely responsible for the increased selectivity towards NH3. These results indicate the importance of limiting the exposure of reduced sulfur species to Pd-based catalysts, especially when treating contaminants like NO3 −, where product selectivity is a priority.
Technology integration for instructional improvement: The impact of professional development

Technology purchased for use in the classroom often goes unused. We identify a primary reason for the lack of technology integration as ineffectively developed professional development opportunities for teachers. Then we recommend a sustained, administrative-supported and mentor-supported approach to professional development as an alternative to the ineffective traditional approach. The importance of considering the role of teachers' attitudes and beliefs on their use of technology in professional development is also discussed.
Transcriptional Genomics Associates FOX Transcription Factors With Human Heart Failure

Background— Specific transcription factors (TFs) modulate cardiac gene expression in murine models of heart failure, but their relevance in human subjects remains untested. We developed and applied a computational approach called transcriptional genomics to test the hypothesis that a discrete set of cardiac TFs is associated with human heart failure. Methods and Results— RNA isolates from failing (n=196) and nonfailing (n=16) human hearts were hybridized with Affymetrix HU133A arrays, and differentially expressed heart failure genes were determined. TF binding sites overrepresented in the −5-kb promoter sequences of these heart failure genes were then determined with the use of public genome sequence databases. Binding sites for TFs identified in murine heart failure models (MEF2, NKX, NF-AT, and GATA) were significantly overrepresented in promoters of human heart failure genes (P<0.002; false discovery rate 2% to 4%). In addition, binding sites for FOX TFs showed substantial overrepresentation in both ad...
Population structural changes in single species of flour beetles with ozone as an environmental factor

1. Although a 5 hour monthly exposure for 16 months to 45.0 ppm O3 at 0.3l/min. reduced the numbers of individuals in single-species populations ofT. confusum andT. castaneum, it did not result in elimination or a significant decrease in numbers which would be useful in a control program for these flour beetle species. 2. During the first 5 months of culture, ozone had a stimulating effect on productivity ofT. confusum; a similar effect was not noted forT. castaneum. 3. Control of flour beetles by ozone in stored-products seems unlikely; however, ozone in an integrated control program with ionizing radiation might prove beneficial. 4. Biological interpretations are offered to explain the responses of these species to ozone exposures. 5. Further research is warranted to define ozone modifications in pest control programs and on stored-productsper se.
Lower levels of glutathione in the brains of secondary progressive multiple sclerosis patients measured by 1H magnetic resonance chemical shift imaging at 3 T.

Background: Disability levels for patients with secondary progressive multiple sclerosis (SPMS) often worsen despite a stable MRI T2 lesion burden. The presence of oxidative stress in the absence of measurable inflammation could help explain this phenomenon. In this study, the assessment of an in vivo marker of oxidative stress, cerebral glutathione (GSH), using magnetic resonance chemical shift imaging (CSI) is described, and GSH levels were compared in patients with SPMS and healthy controls.Objective: To assess whether GSH, a key antioxidant in the brain, is lower in the SPMS patients compared to matched controls.Methods: Seventeen patients with SPMS (Expanded Disability Status Scale = 4.0–7.0; length of MS diagnosis = 19.4 ± 7 years) and 17 age- and gender-matched healthy controls were studied. GSH levels were measured in the fronto-parietal regions of the brain using a specially designed magnetic resonance spectroscopy technique, CSI of GSH, at 3T.Results: The levels of GSH were lower for SPMS patien...
Dysphoric mood in young children referred to a child development unit.

Seventeen parents of 100 young children referred to a child development unit reported predominantly dysphoric mood in their children. The dysphoric group exhibited a cluster of depressive symptoms and also differed from the nondysphorics on referral problems, family history items, I.Q., and other measures.
Neoadjuvant androgen ablation prior to radiotherapy for prostate cancer: Reducing the potential morbidity of therapy

Objectives To determine the impact of neoadjuvant hormonal therapy (NHT) prior to conformal radiotherapy on the reduction of volume of normal tissue structures exposed to high doses of radiation therapy and to evaluate the overall late toxicity and response to treatment among patients treated with this approach.