cssi/SciSciGPT-SciSciNet huggingface dataset:
Chemical cardiac sympathetic denervation hampers defibrillation in the dog.

Cardiac Sympathetic Denervation and Defibrillation. Introduction: Cardiac defibrillation is influenced by several physical and nonphysical factors. Previous animal studies have shown that beta-adrenergic stimulation facilitates the process of defibrillation. The purpose of this study was to examine the effects of chemical sympathetic denervation on the ability to defibrillate the canine heart. Methods and Results: Twelve chronically instrumented dogs underwent serial measurements of the energy required to defibrillate the heart, ten before and after treatment with 50 mg/kg 6-hydroxydopaniine (6-OHDA). Two of the animals received 1% ascorbic acid in 0.9% saline solution (the vehicle) only, and three dogs received the vehicle followed several weeks later by 6-OHDA. Following treatment with 6-OHDA, the energy to defibrillate the heart rose from 11.9 ± 7.4 J (baseline 1) and 14.3 ± 8.7 J (baseline 2) to 23.3 ± 10.8 J (P < 0.01 and < 0.05, respectively). In contrast, following saline administration, no significant change was measured in the energy required to defibrillate the heart. After 6-OHDA, 5 of the 10 animals could not he defibrillated versus none of 5 after saline treatment (Chi square 3.750, P = 0.053). In surviving animals, a return of measurements to, or toward, baseline was measured after active treatment. Conclusions: In this chronically instrumented, closed chest animal model, chemical sympathetic denervation with 6-OHDA hampered the process of cardiac defibrillation. These results support previous observations of a modulating effect of this process by adrenergic activity.
Polycyclic aromatic compounds in Northwest Atlantic COD (Gadus morhua)

Abstract Twenty-seven polycyclic aromatic compounds (PAC) including polycyclic aromatic hydrocarbons (PAH) and polycyclic aromatic sulphur heterocycles were analysed in muscle, liver and ovaries of cod (Gadus morhua) from the Northwest Atlantic. These PAC include the 16 PAH priority pollutants (EPA recommendation), alkylated naphthalenes, phenanthrene-anthracenes as well as dibenzothiophenes (DBT). Aromatics were undetectable in muscle. One of the analysed PAC was detected in one ovary and six in another ovary sample. Liver samples contained between 1 and 9 PAC. Of the parental PAH, only acenapthene (18 ng/g, dry weight), fluorene (28 ng/g) and chrysene (22 ng/g) were detected once each in two liver samples, while fluorene (72 ng/g) was detected once in one of the ovaries. C-2 DBT was the major component in ovaries and liver (8–86 ng/g), while C-3 and C-4 alkylated phenanthrene-anthracene (12–78 ng/g) were the next major components detected in the liver samples. This is the first detailed study of PAH and PAC in finfish from the Northwest Atlantic.
Asymptotic stability of nonlinear multiparameter singularly perturbed systems

Sufficient conditions are derived to guarantee the asymptotic stability of a class of nonlinear singularly perturbed systems with several perturbation parameters of the same order. Estimates of the region of attraction and bounds on the small parameters are obtained.
Formation of Histamine-containing Dinucleotides by Enzymatic Interaction of Histamine and Coenzyme I

DIPHOSPHOPYRIDINE nucleotide (coenzyme I) reacts, in the presence of a soluble purified preparation of diphosphopyridine nucleotidase1, with 4-amino-5-carboxamido-imidazole yielding the pyrophosphate of adenosine-5′-phosphate and of the 4-amino-5-carboxamido-imidazole ribotide2,3. The latter may serve as a precursor in the biosynthesis of inosinic acid4.
Differential effects of DNA supercoiling on radical-mediated DNA strand breaks

Supercoiling is an important feature of DNA physiology in vivo. Given the possibility that the reaction of genotoxic molecules with DNA is affected by the alterations in DNA structure and dynamics that accompany superhelical tension, we have investigated the effect of torsional tension on DNA damage produced by five oxidizing agents: gamma-radiation, peroxynitrite, Fe2+/ EDTA/H2O2, Fe2+/H2O2, and Cu2+/H2O2. With positively supercoiled plasmid DNA prepared by a recently developed technique, we compared the quantity of strand breaks produced by the five agents in negatively and positively supercoiled pUC19. It was observed that strand breaks produced by gamma-radiation, peroxynitrite, and Fe2+/EDTA/H2O2 were insensitive to DNA superhelical tension. These results are consistent with a model in which chemicals that generate highly reactive intermediates (e.g., hydroxyl radical), but do not interact directly with DNA, will be relatively insensitive to the changes in DNA structure and dynamics caused by superhelical tension. In the case of Fe2+ and Cu2+, metals that bind to DNA, only Cu2+/H2O2 proved to be sensitive to DNA superhelical tension. Strand breaks produced by Cu2+/H2O2 in the positively supercoiled substrate occurred at lower Cu concentrations than in negatively supercoiled DNA. Furthermore, a sigmoidal Cu2+/H2O2 damage response was observed in the negatively supercoiled substrate but not in positively supercoiled DNA. The results with Cu2+ suggest that the redox activity, DNA binding orientation, or DNA binding affinity of Cu1+ or Cu2+ is sensitive to superhelical tension, while the results with the other oxidizing agents warrant further investigation into the role of supercoiling in base damage.
Antiseptal brain antibody in IgG of schizophrenic patients

On the hypothesis that schizophrenia is an immunological disorder in which antibody is produced against a unique antigen sequestered principally or exclusively in the septal region of the brain, we used crossed-immunoelectrophoresis (CIE) to evaluate reactivity of a gamma G immunoglobulin (IgG) fraction from serum of schizophrenic patients and nonschizophrenic control subjects with homogenates of tissues of septal region, hippocampus, vermal cerebellum, frontal cortex, and liver of rhesus monkeys. When IgG fractions of unmedicated schizophrenic patients and schizophrenic patients who had received neuroleptic medication for less than 24 hr were tested against septal region homogenate, a precipitin arc was identified, indicating a positive result, with more than 95% of the fractions. In contrast, IgG fractions of schizophrenic patients who had received neuroleptic medication for more than 24 hr were rarely positive. When schizophrenic fractions that tested positive against septal region homogenate were tested against homogenates of the other tissues, they were negative. Fractions of all nonschizophrenic control subjects were negative against all homogenates.
Surfistas Locales: Transnationalism and the Construction of Surfer Identity in Nicaragua:

Surfers have created their own subculture, which has been associated with concepts such as environmentalism, masculinity, place, and nonconformity, yet the increasing global reach of their sport has created transnational surf communities that bring into question the definition of what it means to be a “local” surfer. This ethnographic study examines identity construction in local Nicaraguan surfers, the ways in which their subculture has formed within a transnational context, how they accept/reject resident foreign surfers, and how foreign surfers see themselves in Nicaragua’s globalized surf space. The findings indicate that Nicaraguan surfers have formed their own local surf subculture from globalized influences, and determining whether foreigners are accepted or rejected from this subculture depends on a complex set of factors related to their relationship with local surfers and the local indigenous community.
Preparation and in vivo evaluation of a novel stabilized linker for 211At labeling of protein

Significant improvement of in vivo stability of 211At-labeled radioimmunoconjugates achieved upon employment of a recently reported new linker, succinimidyl N-2-(4-[211At]astatophenethyl)succinamate (SAPS), prompted additional studies of its chemistry. The 211At radiolabeling of succinimidyl N-2-(4-tributylstannylphenethyl)succinamate (1) was noted to decline after storage at -15 degrees C for greater than 6 months. Compound 1 was found to degrade via a ring closure reaction with the formation of N-2-(4-tributylstannylphenethyl)succinimide (3), and a modified procedure for the preparation of 1 was developed. The N-methyl structural analog of 1, succinimidyl N-2-(4-tributylstannylphenethyl)-N-methyl succinamate (SPEMS), was synthesized to investigate the possibility of improving the stability of reagent-protein linkage chemistry. Radiolabeling of SPEMS with 211At generates succinimidyl N-2-(4-[211At]astatophenethyl)-N-methyl succinamate (Methyl-SAPS), with yields being consistent for greater than 1 year. Radiolabelings of 1 and SPEMS with 125I generated succinimidyl N-2-(4-[125I]iodophenethyl)succinamate (SIPS) and succinimidyl N-2-(4-[125I]iodophenethyl)-N-methyl succinamate (Methyl-SIPS), respectively, and showed no decline in yields. Methyl-SAPS, SAPS, Methyl-SIPS and SIPS were conjugated to Herceptin for a comparative assessment in LS-174T xenograft-bearing mice. The conjugates of Herceptin with Methyl-SAPS or Methyl-SIPS demonstrated immunoreactivity equivalent to if not superior to the SAPS and SIPS paired analogs. The in vivo studies also revealed that the N-methyl modification resulted in a superior statinated product.
Perceiving visual expansion without optic flow

When an observer moves forward in the environment, the image on his or her retina expands. The rate of this expansion conveys information about the observer's speed1 and the time to collision2,3,4. Psychophysical5,7 and physiological8,9 studies have provided abundant evidence that these expansionary motions are processed by specialized mechanisms in mammalian visual systems. It is commonly assumed that the rate of expansion is estimated from the divergence of the optic-flow field (the two-dimensional field of local translational velocities)10,11,12,13,14. But this rate might also be estimated from changes in the size (or scale) of image features15. To determine whether human vision uses such scale-change information, we have synthesized stochastic texture stimuli in which the scale of image elements increases gradually over time, while the optic-flow pattern is random. Here we show, using these stimuli, that observers can estimate expansion rates from scale-change information alone, and that pure scale changes can produce motion after-effects. These two findings suggest that the visual system contains mechanisms that are explicitly sensitive to changes in scale.
Approaches to learning by undergraduate students: A longitudinal study

The purpose of this study was to determine if the success of students at a highly selective combined baccalaureate/MD degree program is affected by their different approaches to learning. One hundred freshmen students at the University of Missouri-Kansas City School of Medicine (UMKC) were surveyed using the Lancaster Approaches to Studying Inventory (short survey by Entwistle, 1981). Analysis of the data indicates that the learning styles of students from this combined-degree school tend to be similar to the learning styles of students from other baccalaureate-degree programs in medicine. It appears there are some relationships between approaches to learning and success in the program. This association between learning styles and success also appears to be related to gender differences.
Structural phase transition in epitaxial perovskite films

Three different film systems have been systematically investigated to understand the effects of strain and substrate constraint on the phase transitions of perovskite films. In $\mathrm{Sr}\mathrm{Ti}{\mathrm{O}}_{3}$ films, the phase transition temperature ${T}_{c}$ was determined by monitoring the superlattice peaks associated with rotations of $\mathrm{Ti}{\mathrm{O}}_{6}$ octahedra. It is found that ${T}_{c}$ depends on both $\mathrm{Sr}\mathrm{Ti}{\mathrm{O}}_{3}$ film thickness and $\mathrm{Sr}\mathrm{Ru}{\mathrm{O}}_{3}$ buffer layer thickness. However, lattice parameter measurements showed no sign of the phase transitions, indicating that the tetragonality of the $\mathrm{Sr}\mathrm{Ti}{\mathrm{O}}_{3}$ unit cells was no longer a good order parameter. This signals a change in the nature of this phase transition, the internal degree of freedom is decoupled from the external degree of freedom. The phase transitions occur even without lattice relaxation through domain formation. In $\mathrm{Nd}\mathrm{Ni}{\mathrm{O}}_{3}$ thin films, it is found that the in-plane lattice parameters were clamped by the substrate, while the out-of-plane lattice constant varied to accommodate the volume change across the phase transition. This shows that substrate constraint is an important parameter for epitaxial film systems, and is responsible for the suppression of external structural change in $\mathrm{Sr}\mathrm{Ti}{\mathrm{O}}_{3}$ and $\mathrm{Nd}\mathrm{Ni}{\mathrm{O}}_{3}$ films. However, in $\mathrm{Sr}\mathrm{Ru}{\mathrm{O}}_{3}$ films we observed domain formation at elevated temperature through x-ray reciprocal space mapping. This indicated that internal strain energy within films also played an important role, and may dominate in some film systems. The final strain states within epitaxial films were the result of competition between multiple mechanisms and may not be described by a single parameter.
Wind–Evaporation Feedback and Abrupt Seasonal Transitions of Weak, Axisymmetric Hadley Circulations

Abstract For an imposed thermal forcing localized off the equator, it is known that conservation of absolute angular momentum in axisymmetric flow produces a nonlinear response once the forcing exceeds a critical amplitude. It is shown here that, for a moist atmosphere in convective quasi-equilibrium, the combination of wind-dependent ocean surface enthalpy fluxes and zonal momentum advection can provide a separate feedback that causes the meridional flow to evolve nonlinearly as a function of a sea surface temperature (SST) forcing, even if an angular momentum–conserving response is not achieved. This wind–evaporation feedback is examined in both an axisymmetric primitive equation model and a simple model that retains only a barotropic and single baroclinic mode. Only SST forcings that do not produce an angular momentum–conserving response are examined here. The wind–evaporation feedback is found to be inhibited in models with linear dynamics because the barotropic component of the Hadley circulation, wh...
Relationship of hardiness, grit, and emotional intelligence to internet addiction, excessive consumer spending, and gambling

Hardiness is conceptualized as the courage that protects against such effects of denial and avoidance of life’s stressors as internet addiction, excessive consumer spending, and gambling. Also relevant protections may be grit, and emotional intelligence (EI). This study compares the relative effectiveness of these three aspects of personality on the negative performance considerations mentioned. All the measures of personality and performance were completed by a sample of undergraduates. The results showed that, as expected, hardiness was a better negative predictor of internet addiction and excessive consumer spending than were grit and emotional intelligence. There is also some indication that hardiness was also a better protection against gambling.
Formation of new oxide glasses by laser spin melting and free fall cooling

Abstract Glass spherules have been made by laser spin melting and free fall cooling techniques from ceramic rods. Thus, the materials were not in contact with a container at any time. Glasses of 100 to 800 μm diameter were formed from at least 80 wt% of the oxides Al2O3, Ga2O3, In2O3, La2O3, ZrO2, HfO2, Nb2O5 and Ta2O5 with 20 or less weight percent of CaO + SiO2. The best glass formers were in the Nb2O5- and Ta2O5-based systems. The indices of refraction of most of the glasses and Abbe numbers of many were measured.
Overexpression of Dimethylarginine Dimethylaminohydrolase 1 Attenuates Airway Inflammation in a Mouse Model of Asthma

Levels of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, are increased in lung, sputum, exhaled breath condensate and plasma samples from asthma patients. ADMA is metabolized primarily by dimethylarginine dimethylaminohydrolase 1 (DDAH1) and DDAH2. We determined the effect of DDAH1 overexpression on development of allergic inflammation in a mouse model of asthma. The expression of DDAH1 and DDAH2 in mouse lungs was determined by RT-quantitative PCR (qPCR). ADMA levels in bronchoalveolar lavage fluid (BALF) and serum samples were determined by mass spectrometry. Wild type and DDAH1-transgenic mice were intratracheally challenged with PBS or house dust mite (HDM). Airway inflammation was assessed by bronchoalveolar lavage (BAL) total and differential cell counts. The levels of IgE and IgG1 in BALF and serum samples were determined by ELISA. Gene expression in lungs was determined by RNA-Seq and RT-qPCR. Our data showed that the expression of DDAH1 and DDAH2 was decreased in the lungs of mice following HDM exposure, which correlated with increased ADMA levels in BALF and serum. Transgenic overexpression of DDAH1 resulted in decreased BAL total cell and eosinophil numbers following HDM exposure. Total IgE levels in BALF and serum were decreased in HDM-exposed DDAH1-transgenic mice compared to HDM-exposed wild type mice. RNA-Seq results showed downregulation of genes in the inducible nitric oxide synthase (iNOS) signaling pathway in PBS-treated DDAH1-transgenic mice versus PBS-treated wild type mice and downregulation of genes in IL-13/FOXA2 signaling pathway in HDM-treated DDAH1-transgenic mice versus HDM-treated wild type mice. Our findings suggest that decreased expression of DDAH1 and DDAH2 in the lungs may contribute to allergic asthma and overexpression of DDAH1 attenuates allergen-induced airway inflammation through modulation of Th2 responses.
Accountable to what?: Professional orientations towards accountability-based juvenile justice

Formal philosophical and procedural aspects of American juvenile justice have been transformed over the past half-century by `accountability' movements. Yet the meaning of accountability in juvenil...
Trypanosoma brucei gambiense: growth in vitro of bloodstream forms inhibited by dichlorodiammineplatinum (II) compounds and hypolipidemic drugs.

Infectious bloodstream forms of Trypanosoma brucei gambiense were grown in microcultures of murine bone marrow cells in 96-well tissue culture plates. Limiting dilution studies showed that fewer than 10 cultured trypanosomes developed into populations of about 5 × 104 parasites per well in a week. Bloodstream parasites were reisolated with high efficiency from mice infected with cultured parasites; fewer than 10 bloodstream parasites successfully established a trypanosome population in a microculture. Both the cis and trans isomers of dichlorodiammineplatinum (II) (cisplatin and transplatin) and a hypolipidemic agent, Wy 14643, were found to have activity against T. b. gambiense growing in microcultures. The minimum concentration of drug necessary to completely eliminate parasites from microcultures was 4 μM for cisplatin, 40 μM for transplatin, and 500 μM for Wy 14643. A preformed complex of cisplatin and bovine serum albumin and another hypolipidemic agent, chlofibric acid, were inactive. This culture system should be useful for rapid screening of large numbers of compounds for trypanocidal activity.
Email small group peer review revisited

Abstract Attention to email exchanged among a small group of student peers supercedes discussion of networked computer labs and is distinguished from research on collaborative classroom work in general, on online peer tutoring in writing centers, on email communication in online professional writing courses, and on online discourse in general. Email peer response within small groups is different from larger-scale, one-to-many computer-based communication tools (CBCT) on class mailing lists, bulletin boards, blogs, and wikis on the one hand and smaller-scale, one-to-one email exchange between an individual student and a peer tutor on the other hand. The benefits of assignments that require small groups to respond electronically and asynchronously to each other's drafts are analyzed and illustrated: rhetorical/thematic, discursive/environmental, technological, logistical/time management. The practicalities of students’ exchange of drafts, deadlines, and other guidelines are explained and illustrated in typical student email responses and model instructor handouts.
Realization of time gating by use of spatial filtering

A method for simulating conventional time gating in low-coherence optical imaging processes in highly scattering media is given. The method uses monochromatic instead of broadband light, and spatial filtering is substituted for time gating. The process enables the study of imaging techniques in scattering media to be carried out in an easy and highly controllable way. Experimental results are given.
Interaction between electrostatic whistlers and electron holes in the auroral region

[1] Electron holes have been discovered in several key regions of the magnetosphere in the past few years. These small-scale structures seem to play an important role in the global dynamics of the magnetosphere, being most often associated with parallel electron beams and strong ion heating, as shown by the Fast Auroral Snapshot (FAST) spacecraft in the auroral region. Electrostatic whistler waves (EWW) in the VLF frequency range are often associated with these electron holes, suggesting that the generation of whistler waves is related to the holes. We present a model of the interaction between EWW and electron holes. Our analysis is based on the mechanical description of the energy exchange between particles and waves; by the use of the Hamiltonian formalism, action-angle variables, and canonical perturbation theory, we show that trapped electrons, owing to their periodic motion in the potential structure of the electron hole, enter into a resonance with the wave and can destabilize it at large parallel phase velocity relative to the electron thermal velocity. We derive the growth rate of EWW and compare our model with previous ones. Application to FAST observations shows that this linear instability is able to generate EWW with a normalized growth rate γ/ω varying from a few percents at low frequency (above the lower-hybrid frequency) up to ∼10% at higher frequency (below the electron plasma frequency).
Testing the standard model in B-->K (*) l + l -

We study the potential of {ital B}{r_arrow}{ital K}{sup (*)}{ital l}{sup +}{ital l}{sup {minus}} decays as tests of the standard model. After discussing the reliability of theoretical predictions for the hadronic mix elements involved, we examine the impact of different new physics scenarios on various observables. We show that the angular information in {ital B}{r_arrow}{ital K}{sup *}{ital l}{sup +}{ital l}{sup {minus}} together with the dilepton mass distribution can highly constrain new physics. This is particularly true in the large dilepton mass region, where reliable predictions for the hadronic matrix elements can be made with presently available data. As illustrative examples, we compare the standard model predictions with those of two-Higgs-doublet models as well as top-quark color models, all of which give distinct signals in this region.
Full thermoelectric characterization of InAs nanowires using MEMS heater/sensors.

Precise measurements of a complete set of thermoelectric parameters on a single indium-arsenide nanowire (NW) have been performed using highly sensitive, micro-fabricated sensing devices based on the heater/sensor principle. The devices were fabricated as micro electro-mechanical systems consisting of silicon nitride membranes structured with resistive gold heaters/sensors. Preparation, operation and characterization of the devices are described in detail. Thermal decoupling of the heater/sensor platforms has been optimized reaching thermal conductances as low as with a measurements sensitivity below . The InAs NWs were characterized in terms of thermal conductance, four-probe electrical conductance and thermopower (Seebeck coefficient), all measured on a single NW. The temperature dependence of the parameters determining the thermoelectric figure-of-merit of an InAs NW was acquired in the range 200–350 K featuring a minor decrease of the thermal conductivity from 2.7 W to 2.3 W .
FOXO3A regulates peroxiredoxin III expression in human cardiac fibroblasts.

Human cardiac fibroblasts are protected from oxidative stress triggered by inflammation after myocardial injury (Li, P. F., Dietz, R., and von Harsdorf, R. (1999) FEBS Lett. 448, 206–210) by expressing potent antioxidant defenses such as superoxide dismutases, catalases, glutathione-peroxidases, and peroxiredoxins. Recently the transcription factor FOXO3A has been shown to increase resistance to oxidative stress by up-regulation of mitochondrial superoxide dismutase and peroxisomal catalase (Kops, G. J., Dansen, T. B., Polderman, P. E., Saarloos, I., Wirtz, K. W., Coffer, P. J., Huang, T. T., Bos, J. L., Medema, R. H., and Burgering, B. M. (2002) Nature 419, 316–321; Nemoto, S., and Finkel, T. (2002) Science 295, 2450–2452). We hypothesized that FOXO3A also regulates the expression of Prx III, the mitochondrial peroxiredoxin, in human cardiac fibroblasts. We found that depletion of FOXO3A leads to a dramatic reduction of Prx III mRNA and protein in serum-deprived human cardiac fibroblasts. These data suggest that endogenous FOXO3A is necessary for base-line expression of Prx III. Next, we identified two putative FOXO3A DNA binding sites in Prx III promoter at –267 and –244 nucleotides relative to the start codon. We demonstrated that both sequences are required for binding of endogenous FOXO3A to the Prx III promoter by performing electromobility shift assays and chromatin immunoprecipitation assays. Inhibition of endogenous FOXO3A by insulin growth factor 1 prevented binding of FOXO3A to Prx III promoter. In contrast, overexpression of FOXO3A increased Prx III promoter activity. Furthermore, depletion of Prx III was associated with enhanced apoptosis and oxidative stress after serum deprivation. We conclude that FOXO3A mediates Prx III expression, and this may play a critical role in the resistance to oxidative stress in cardiac fibroblasts.
The initial effects of an elbow mobilization with movement technique on grip strength in subjects with lateral epicondylalgia.

This preliminary study indicates the proportion of patients with lateral epicondylalgia that demonstrate a favourable initial response to a manual therapy technique - the mobilization with movement (MWM) for tennis elbow. Twenty-five subjects with lateral epicondylalgia participated. In a one-group pretest - post-test design, we measured (1) pain with active motion, (2) pain-free grip strength and, (3) maximum grip strength before and after a single intervention of MWM. Results of the study indicate that MWM was effective in allowing 92% of subjects to perform previously painful movements pain-free, and improving grip strength immediately afterwards. Significant differences were found between the grip strength of the affected and unaffected limbs prior to the intervention. Both pain-free grip strength and maximum grip strength of the affected limb increased significantly following the intervention. Pain-free grip strength increased by a greater magnitude than maximum grip strength. It can be concluded that MWM is a promising intervention modality for the treatment of patients with Lateral Epicondylalgia. Pain-free grip strength is a more responsive measure of outcome than maximum grip strength for patients with Lateral Epicondylalgia. Further research is warranted to investigate the long-term effectiveness of MWM in the treatment of impairment and disability resulting from Lateral Epicondylalgia.
Energy dispersive x-ray diffraction of micro-crystals at ultrahigh pressures

Abstract Ultrahigh pressures and temperatures in diamond-anvil cells are achieved at the expense of reducing sample volume. The capability of x-ray diffraction with high spatial resolution is most fundamental for probing microscopic samples at the maximum P-T and for minimizing the effect of gradients. Polychromatic synchrotron radiation with energy dispersive x-ray diffraction is ideal for the development of new classes of structural microprobes. Primary x-ray beams down to 3 microns can be produced with microbeam slit systems and microfocusing optical devices. The microprobe can be routinely used for a variety of high-pressure experiments, including single-crystal x-ray diffraction above 50 GPa, polycrystal-line diffraction above 300 GPa, deviatoric strain measurements, and diffraction at simultaneous high pressure and temperature.
A ventilation-exchange bougie for fibreoptic intubations with the laryngeal mask airway.

Summary The ventilation-exchange bougie is a new airway device which can be mounted on a fibreoptic laryngoscope for passage through the larynx into the trachea via a laryngeal mask airway. Subsequent removal of the fibreoptic laryngoscope and laryngeal mask airway allows a tracheal tube to be railroaded into position over the ventilation-exchange bougie. This study described the use of this technique for elective tracheal intubation in two groups of 12 subjects in whom difficulty with intubation was not expected. All the subjects were successfully intubated by one of two anaesthetists, one experienced and the other inexperienced with fibreoptic intubation techniques. Neither had had prior experience with the ventilation-exchange bougie. Because ventilation was maintained throughout the procedure, intubation did not need to be hurried. Cusum analysis confirmed the impression of a learning curve and the technique could be considered learnt after four and six intubations for the experienced and inexperienced fibreoptic larvngoscopists respectively. No difficulty was found either in intubating the larynx with the fibreoptic laryngoscope and ventilation-exchange bougie or when railroading the tracheal tube over the ventilation-exchange bougie. It is suggested that this new device could have an important role in teaching fibreoptic techniques, management of the difficult airway and failed intubations.
A theory of speech perception: II

The author’s theory of speech perception, as applied to time-dependent speech sounds, leads to many testable predictions. While some of these predictions are consistent with conventional knowledge, others are new and quite unexpected. A few are in contradiction to long accepted experimental results. A computer-aided experimental program, designed to test the theory, wholly supported these predictions. In view of this outcome, it seems desirable to test other predictions of the theory and to reexamine some conventionally accepted views in order to arrive at a more comprehensive theory of speech. The present findings indicate that, apart from categorization, consonants are similar to vowels: they exhibit parallel organizations and transformation properties.
Efficient Methods to Compute Genomic Predictions

Efficient methods for processing genomic data were developed to increase reliability of estimated breeding values and to estimate thousands of marker effects simultaneously. Algorithms were derived and computer programs tested with simulated data for 2,967 bulls and 50,000 markers distributed randomly across 30 chromosomes. Estimation of genomic inbreeding coefficients required accurate estimates of allele frequencies in the base population. Linear model predictions of breeding values were computed by 3 equivalent methods: 1) iteration for individual allele effects followed by summation across loci to obtain estimated breeding values, 2) selection index including a genomic relationship matrix, and 3) mixed model equations including the inverse of genomic relationships. A blend of first- and second-order Jacobi iteration using 2 separate relaxation factors converged well for allele frequencies and effects. Reliability of predicted net merit for young bulls was 63% compared with 32% using the traditional relationship matrix. Nonlinear predictions were also computed using iteration on data and nonlinear regression on marker deviations; an additional (about 3%) gain in reliability for young bulls increased average reliability to 66%. Computing times increased linearly with number of genotypes. Estimation of allele frequencies required 2 processor days, and genomic predictions required <1 d per trait, and traits were processed in parallel. Information from genotyping was equivalent to about 20 daughters with phenotypic records. Actual gains may differ because the simulation did not account for linkage disequilibrium in the base population or selection in subsequent generations.
Deformable image registration with content mismatch: a demons variant to account for added material and surgical devices in the target image

Fast, accurate, deformable image registration is an important aspect of image-guided interventions. Among the factors that can confound registration is the presence of additional material in the intraoperative image - e.g., contrast bolus or a surgical implant - that was not present in the prior image. Existing deformable registration methods generally fail to account for tissue excised between image acquisitions and typically simply “move” voxels within the images with no ability to account for tissue that is removed or introduced between scans. We present a variant of the Demons algorithm to accommodate such content mismatch. The approach combines segmentation of mismatched content with deformable registration featuring an extra pseudo-spatial dimension representing a reservoir from which material can be drawn into the registered image. Previous work tested the registration method in the presence of tissue excision (“missing tissue”). The current paper tests the method in the presence of additional material in the target image and presents a general method by which either missing or additional material can be accommodated. The method was tested in phantom studies, simulations, and cadaver models in the context of intraoperative cone-beam CT with three examples of content mismatch: a variable-diameter bolus (contrast injection); surgical device (rod), and additional material (bone cement). Registration accuracy was assessed in terms of difference images and normalized cross correlation (NCC). We identify the difficulties that traditional registration algorithms encounter when faced with content mismatch and evaluate the ability of the proposed method to overcome these challenges.
Discrepancy between in vitro and in vivo antifungal activity of albendazole

Albendazole has in vitro activity against Cryptococcus neoformans and reduced in vitro activity for albendazole when compared with Candida albicans. The major metabolite of albendazole, albendazole sulphoxide showed no in vitro activity against isolates of either fungus. Immunocompetent mice infected intravenously (i.v.) with C. albicans were treated with albendazole doses of 20-600 mg kg-1 per day in noble agar or sesame oil for per oral (PO) administration, or 80 mg kg-1 per day in DMSO for intraperitoneal (i.p.) and i.v. administration for 10 days, and were observed for survival. Mice infected with C. neoformans intracranially received albendazole in daily doses of 600 mg kg-1 prepared in DMSO (i.p.) or peanut butter/rat chow (PO) for 10 days and were observed for survival. Mortality was not different between the treated and control animals in any study. Plasma samples from uninfected mice dosed with similar formulations and doses of albendazole were analysed by HPLC for albendazole and albendazole sulphoxide. No albendazole could be detected in any sample, while concentrations of albendazole sulphoxide (286-8697 ng ml-1) were observed in all samples. These data suggest that the absence of in vivo activity for albendazole is due to rapid conversion to the inactive albendazole sulphoxide metabolite.
Bovine papilloma virus (BPV)-encoded E1 protein contains multiple activities required for BPV DNA replication.

Abstract Replication of bovine papilloma virus (BPV) DNA requires two virus-encoded proteins, E1 and E2, while all other proteins are supplied by the host cell. Here, we describe the isolation of the E1 protein and show that it is a multifunctional protein. Purified E1 protein was required for the in vitro replication of BPV origin-containing DNA by extracts of mouse cells, as reported [Yang, L., Li, R., Mohr, I. J., Clark, R. & Botchan, M. R. (1991) Nature (London) 353, 628-632]. In addition, the E1 protein cosedimented with a number of other activities including (i) DNA helicase activity, (ii) BPV origin-containing DNA-specific binding activity, (iii) DNA-dependent ATPase activity, and (iv) BPV origin-specific unwinding of superhelical DNA. The E1 protein, acting as a helicase, moved in the 3'-->5' direction, like simian virus 40 (SV40) large tumor antigen, which plays a pivotal role in SV40 DNA replication. However, unlike the SV40 large tumor antigen, the helicase activity of E1 was stimulated 5-fold by the presence of a fork structure at the junction between single-stranded and double-stranded DNA and was supported efficiently by all eight nucleoside triphosphates. The E1-catalyzed ATPase activity required the presence of single-stranded or double-stranded DNAs.
Ancient lithospheric lherzolite xenolith in alkali basalt from Baja California

Abstract K, Rb, Sr contents and Sr isotopic composition are reported for (1) the coexisting silicate minerals of a spinel lherzolite xenolith, (2) the whole rock xenolith, and (3) the host alkali basalt from the Pleistocene-Recent San Quintin volcanic field in Baja California. The data also include major element chemistry of the four mineral phases of the xenolith. The olivine-spinel and pyroxene thermometers indicate that the last temperature of equilibration of the xenolith in the upper mantle was about 1100°C. K, Rb, and Sr abundances are extremely low in the minerals of the xenolith, in contrast with the general enrichment of Ca, Al, Fe and Na in the whole xenolith. Furthermore, the abundance levels and the 87 Sr/ 86 Sr ratios of the minerals are greatly lowered by surface washing of the minerals in 2N cold HCl for three minutes. It is suggested that this is due to grain boundary and surface contamination of the minerals which took place in the upper mantle by a vapor phase deposition, prior to the inclusion of the xenolith in the basalt. The source of the vapor phase must have a 87 Sr/ 86 Sr ratio greater than 0.7070, the highest measured ratio in the unwashed orthopyroxene. Sr in the host alkali basalt has a 87 Sr/ 86 Sr ratio of 0.7031, unrelated to the grain boundary material. The acid-washed minerals, the unwashed minerals and the acid-washed whole rock xenolith show a scatter on a 87 Rb/ 86 Sr versus 87 Sr/ 86 Sr diagram. However, the surface-washed minerals and the whole rock alone define a straight-line relationship with a positive slope, which corresponds to an age 3.4 ± 0.3AE(2σ) for the rock and an initial 87 Sr/ 86 Sr of 0.70057 ± 0.0004 (2σ) . The age of 3.4 AE for the lherzolite is interpreted as its last involvement in a small degree of partial melting and the consequent extreme depletion of the large ion lithophile elements from the constituent minerals.
Geomagnetic polarity transitions in two oriented sediment cores from the northwest Pacific

Abstract The paths described by the geomagnetic poles during Jaramillo and Olduvai polarity transitions, as recorded in two oriented sediment cores from the northwest Pacific Ocean, closely resemble those previously determined in another deep-sea sediment core from the Indian Ocean. The paths determined for a specific transition appear to be the same in all three cores. There seems, however, to be a unique path associated with each of the three successive transitions studied in all three cores.
Community perceptions and foster care placement: A multi-level analysis

Abstract Signs of physical and social disorder in a community have the potential to influence the perceptions about community, which could, in turn, impact the decision making processes involved in child maltreatment investigations. While extant research has demonstrated a link between community context and child maltreatment rates, there is a dearth of research examining the potential relationship between social conditions in a community and the child welfare system's response to allegations of neglect. This study examined the relationship between child- and community-level characteristics and the decision to place a child in foster care. Two data sets were linked, one containing detailed observations of community conditions (e.g., cigarette and alcohol advertisements) and a second with individual child level information about child welfare investigations. Multilevel models allowed for the simultaneous understanding of child- and community-level characteristics. The results indicated strong associations between individual characteristics and foster care placement. The race or ethnicity of the child was a strong predictor of entry into foster care, and young children were much more likely to be placed in foster care than older children. Furthermore, community factors were also associated with placement into foster care. Specifically, the presence of litter and graffiti and the lack of security signage in a neighborhood appeared to impact the decision making process for foster care.
Sada Yacco in London and Paris, 1900: Le Rêve Réalisé

(1995). Sada Yacco in London and Paris, 1900: Le Reve Realise. Dance Chronicle: Vol. 18, No. 3, pp. 343-404.
Resource Utilization Reduction for Evaluation of Chest Pain in Pediatrics Using a Novel Standardized Clinical Assessment and Management Plan (SCAMP).

Background Chest pain is a common reason for referral to pediatric cardiologists. Although pediatric chest pain is rarely attributable to serious cardiac pathology, extensive and costly evaluation is often performed. We have implemented a standardized approach to pediatric chest pain in our pediatric cardiology clinics as part of a broader quality improvement initiative termed Standardized Clinical Assessment and Management Plans (SCAMPs). In this study, we evaluate the impact of a SCAMP for chest pain on practice variation and resource utilization. Methods and Results We compared demographic variables, clinical characteristics, and cardiac testing in a historical cohort (n=406) of patients presenting to our outpatient division for initial evaluation of chest pain in the most recent pre-SCAMP calendar year (2009) to patients enrolled in the chest pain SCAMP (n=364). Demographic variables including age at presentation, sex, and clinical characteristics were similar between groups. Adherence to the SCAMP algorithm for echocardiography was 84%. Practice variation decreased significantly after implementation of the SCAMP ( P <0.001). The number of exercise stress tests obtained was significantly lower in the SCAM P -enrolled patients compared with the historic cohort (∼3% of patients versus 29%, respectively; P <0.001). Similarly, there was a 66% decrease in utilization of Holter monitors and 75% decrease in the use of long-term event monitors after implementation of the chest pain SCAMP ( P =0.003 and P <0.001, respectively). The number of echocardiograms obtained was similar between groups. Conclusions Implementation of a SCAMP for evaluation of pediatric chest pain has lead to a decrease in practice variation and resource utilization.
Phenomenology and Functioning in First-Episode Schizophrenia

One current view of schizophrenia is that its clinical and functional features show a deteriorating course, particularly for negative symptoms. However, this is difficult to study in chronic patients who have been exposed to pharmacologic treatment and institutionalization. Examining first-episode (FE) patients can help clarify which symptoms are present initially and how the symptom pattern is linked to functioning. We evaluated a sample of 37 FE patients with schizophrenia and compared them to 70 other schizophrenia (OS) patients on standard clinical scales, measures of premorbid functioning, and quality of life. FE patients showed a symptom profile similar to OS patients; in particular, there was no evidence that negative symptoms are less severe in the FE group. Analysis of the clinical data led us to the conclusion that the symptom profile of schizophrenia exists at the outset, that negative symptoms are associated with poor premorbid and current functioning, but that the role of positive symptoms is more complex and may vary in subtypes.
A Novel Method for Mapping Land Cover Changes: Incorporating Time and Space With Geostatistics

Landsat data are now available for more than 30 years, providing the longest high-resolution record of Earth monitoring. This unprecedented time series of satellite imagery allows for extensive temporal observation of terrestrial processes such as land cover and land use change. However, despite this unique opportunity, most existing change detection techniques do not fully capitalize on this long time series. In this paper, a method that exploits both the temporal and spatial domains of time series satellite data to map land cover changes is presented. The time series of each pixel in the image is modeled with a combination of: 1) pixel-specific remotely sensed data; 2) neighboring pixels derived from ground observation data; and 3) time series transition probabilities. The spatial information is modeled with variograms and integrated using indicator kriging; time series transition probabilities are combined using an information-based cascade approach. This results in a map that is significantly more accurate in identifying when, where, and what land cover changes occurred. For the six images used in this paper, the prediction accuracy of the time series improves significantly, increasing from 31% to 61%, when both space and time are considered with the maximum likelihood. The consideration of spatial continuity also reduced unwanted speckles in the classified images, removing the need for any postprocessing. These results indicate that combining space and time domains significantly improves the accuracy of temporal change detection analyses and can produce high-quality time series land cover maps
Passive and Active Maternal Smoking during Pregnancy, as Measured by Serum Cotinine, and Postnatal Smoke Exposure. I. Effects on Physical Growth at Age 5 Years

The authors evaluated the effect of maternal environmental tobacco smoke (ETS) exposure during pregnancy and prenatal maternal smoking on child's height at age 5 years, using serum cotinine as a biomarker of exposure. They also determined the effects of postnatal smoke exposure. Participants included 2,622 women enrolled in the Child Health and Development Studies between 1964 and 1967. Nonsmokers were divided into ETS-exposed (serum cotinine 2-10 ng/ml; n = 77) and not exposed (n = 1,610), and smokers (n = 935) were divided into tertiles based on serum cotinine levels: 0-79, 80-163, and 164-569 ng/ml. Multivariate models adjusting for race, sex, birth order, and maternal height, body mass, education, and age indicated that children of smokers were 0.3, 0.3, and 0.8 cm shorter in the lowest, middle, and highest tertile of serum cotinine, respectively, and children of ETS-exposed women were 0.5 cm taller than those of nonsmokers. Only the children of heavy smokers were significantly shorter than children of nonsmokers; however, this difference disappeared after controlling for birth weight and gestational age. The adjusted heights of children of women who smoked both during and after pregnancy were significantly shorter than those of children of nonsmokers, but this effect also disappeared after controlling for birth weight and gestational age. These results suggest that the effects of smoke exposure on children's height may be explained by the effects of maternal smoking on fetal growth.