Exposing a killer: pathologists angle for anthrax.

The grim reality of bioterrorism struck the United States in 2001 in the wake of the horrific terrorist attacks on the World Trade Center and the Pentagon. Weaponized Bacillus anthracis was directed through the postal service to targeted individuals in media and government institutions resulting in 22 confirmed cases of anthrax with five fatalities, the latter being associated with inhalational exposure. 1 Until the attack, general interest in anthrax was relatively subdued, being largely a veterinary concern, but that has clearly changed as reflected in the pre- and post-2001 publication rates on this topic (Figure 1) ▶ . In the battle against weaponized anthrax, the skills and techniques of pathology have stepped up to the challenge. Figure 1. Annual publication rates of journal articles regarding anthrax. Derived from the National Library of Medicine reference database. Anthrax is among the diseases that have shaped human history. It holds a place in the Judeo-Christian tradition, being purported to be the agent of the sixth of the ten plagues of Egypt, its cutaneous form was described by the Roman poet, Virgil, and through the efforts of Robert Koch and Louis Pasteur it launched broad acceptance of germ theory and vaccination. 2-4 Military strategists quickly recognized the potential of anthrax as a biological weapon with evidence of attempted application at least as early as World War I. 5 Despite international bans on biological warfare, governments with the means have continued research to accentuate the delivery and pathogenicity of this agent. Despite our long historical relationship with anthrax there are still challenges with respect to its biology and pathophysiology that have yet to be overcome; however, the knowledge that has accrued reveals why this organism can be so effectively applied as a bioweapon. Bacillus anthracis is a non-motile, gram-positive bacillus that can survive in spore form in soil for decades. On infecting a mammalian host the spores germinate into vegetative forms at 37°C and if unrestrained, will grow at a tremendous rate leaving a rotting carcass seething with sporulating organisms. Thus, the bacteria only require the living host as a temporary “incubation chamber”. Fortunately, humans are relatively resistant to disseminated infection under conditions of natural exposure but several forms of infection do occur. Bacteriological and clinical aspects of anthrax have been extensively reviewed 2,6,7 and will only be briefly summarized here. The major clinical forms of anthrax are cutaneous, gastrointestinal, inhalational, and meningeal, the latter being the potential result of secondary lymphatic or hematogenous spread after primary infection at one of the other sites. Cutaneous anthrax is the most commonly encountered clinical manifestation under conditions of natural environmental exposure. 8 The spores enter a cut or abrasion where they develop into vegetative forms, causing development of a localized papule followed by non-suppurative vesiculation, ulceration, and finally, formation of a black eschar, which is responsible for the organism’s name, anthracis, meaning “coal-like”. Although cutaneous anthrax can sometimes progress to disseminated disease it is usually self-limited, suggesting that host immunity has adapted to contain the organism when exposed by that route. Gastrointestinal anthrax is considered to be of lower incidence than the cutaneous form but it has been argued that this is due mainly to poor recognition of the disease. 9 However, it is generally agreed that this form of the disease is associated with greater morbidity and mortality. Humans acquire the disease through the ingestion of undercooked B. anthracis-contaminated meat. Spores can penetrate at any site from the oropharynx to the cecum. Analogous to cutaneous anthrax there is ulceration at the site of primary infection with spread to regional lymph nodes and associated hemorrhage and enlargement. As might be predicted, the clinical presentation differs depending on the site of primary penetration. Oropharyngeal anthrax presents with upper gastrointestinal symptoms such as sore throat, dysphagia, fever, edema, and cervical lymphadenopathy. Gastrointestinal anthrax results in a gastroenteritis picture presenting with anorexia, abdominal pain, nausea, vomiting, fever, and diarrhea, which can progress to bloody diarrhea, hematemesis, and ascites, with eventual septicemia. Mortality rates for gastrointestinal anthrax are not clearly established due to lack of controlled epidemiological studies, and reported values range from 4% to 50%. 9 The studies with larger patient numbers suggest that lower values are more accurate, indicating some degree of resistance to gastrointestinal exposure. Inhalational anthrax is by far the most fatal form of primary infection, approaching nearly 100% mortality without medical intervention. Although spores are of ideal size to reach alveolar spaces on inhalation, the lung is likely not a normal route of primary infection under natural conditions. Analysis of airborne spores around fields with anthrax-infested carcasses indicated that spores were associated with larger particles and airborne spore counts were low, making airborne spread of disease unlikely. 10 However, rare episodes of occupational exposure of the lung is well recognized among individuals handling contaminated animal products in closed environments, a condition previously known as “Wool Sorter’s Disease.” 11 Efficient exposure of the lung requires purposeful weaponization of spores so that they remain dispersed and airborne. The high susceptibility of the lung route of infection is likely related to the lack of host organ-based innate resistance and optimal conditions for application of bacterial virulence factors. Hence, weaponized B. anthracis is a highly effective killer on inhalation. Inhaled B. anthracis spores are rapidly phagocytosed by alveolar macrophages and transported to draining lymph nodes of lung and mediastinum. Thus, unlike many other bacteria, B. anthracis uses the lung as a portal of entry rather than a site of primary infection. Within hours after phagocytosis, spores begin to germinate into vegetative cells within lymph nodes; however, spore forms may persist for more than a month (providing the rationale for prolonged post-exposure antibiotic therapy). Vegetative cells multiply rapidly at body temperature with concomitant induction of plasmid-encoded virulence factors. 12 Two plasmids, pXO1 and pXO2, carry the genes for these factors. Plasmid pXO1 codes for three exotoxin components, protective antigen (PA), lethal factor (LF), and edema factor (EF), which combine to form two binary forms, lethal toxin (PA+LF) and edema toxin (PA+EF). The PA is a 735 amino acid polypeptide that facilitates transfer to the cytosol, where the toxins act. Lethal toxin, a zinc metalloproteinase, has the capacity to inactivate mitogen-activated protein kinase kinase (MAPKK) and stimulate the release of sepsis-related cytokines tumor necrosis factor-α and interleukin-1β. Recently, lethal toxin was demonstrated to impair dendritic cell maturation, potentially disrupting the link between innate and adaptive immune recognition. 13 Edema toxin, a calmodulin-dependent adenylate cyclase, increases levels of cyclic AMP, causing impaired neutrophil function and disruption of water balance, resulting in massive tissue edema. The pOX2 plasmid encodes three genes involved in synthesis of a polyglutamyl capsule that protects the vegetative bacteria from phagocytosis. Together, the toxins effectively inhibit host innate and adaptive immune responses, allowing the bacteria to grow unrestrained and overwhelming any resistance. Clinically, inhalational anthrax presents in a biphasic pattern with initial nonspecific flu-like symptoms nausea and vomiting 1 to 4 days after exposure, followed by severe illness with dyspnea, high fever, and shock. The latter symptoms represent a terminal stage and treatment is often ineffective when started at that time. A key radiological finding purported to aid early recognition is mediastinal widening in association with pleural effusion, but the interpreting clinician must also have a high index of suspicion for anthrax exposure. 1,14 Unfortunately, as evidenced in 2001, that suspicion may be absent in the initial days following a bioterror attack and ultimate diagnosis will depend on the investigations of pathologists and laboratorians.