cssi/SciSciGPT-SciSciNet huggingface dataset:
Integration of Just In Time JIT Inventory in Outpatient Pharmacy Information Systems

With the implementation of the recent Healthcare Reform Act and the increased scrutiny on the soaring costs of healthcare, medical plans are looking for ways to optimize workflows and reduce costs. Titan Healthcare is a large non-profit integrated healthcare company located in Arizona, New Mexico, Nevada, Colorado, and Texas. They provide health insurance coverage, and a broad range of comprehensive health care services. Titan Healthcare is engrossed to employ the best strategies to close performance gaps and efficiently manage their operations. To help improve their pharmacy operations, they are seeking to design and implement a new Outpatient Pharmacy Information System which will handle both dispensing and inventory functionality. From a pharmacy inventory perspective, expectations for the system are to significantly reduce inventory costs and increase service levels to their members. Titan Healthcare is currently exploring options for designing the right Outpatient Pharmacy Inventory Management System for its operations.
Current standards and practices in violence risk assessment at a maximum security forensic hospital following a high profile sexual homicide

Often high profile cases impact the standards for ethical practices in forensic psychology and psychiatry. In Minnesota, a high profile sexual homicide allegedly committed by a parolee who was not civilly committed as mentally ill and dangerous or sexually dangerous (likely due to older age) has brought to question the state's risk assessment policies. Due to the high profile nature of this case, the State Operated Forensic Services (SOFS) in Minnesota has attempted to implement heightened standards in their violence risk assessment policy for both mentally ill and dangerous and sexually violent offender populations. The rationale for this article is to outline how the publicity of a high profile sexual homicide can result in changes in a state's risk assessment procedures. The author will briefly outline the revised risk assessment standards within a state operated civil commitment forensic hospital and describe a case study of a civilly committed sex offender who was examined under these new standards. The author will also question whether more is better. Specifically, the question will be asked whether such standards and practices are efficient and necessary given the issues of incremental validity and the fact that there will always be false negatives (an offender who is deemed to be non-dangerous, who ultimately is).
Factorization and resummation for single color-octet scalar production at the LHC

Heavy colored scalar particles appear in a variety of new physics (NP) models and could be produced at the Large Hadron Collider. Knowing the total production cross section is important for searching for these states and establishing bounds on their masses and couplings. Using soft-collinear effective theory, we derive a factorization theorem for the process $pp\ensuremath{\rightarrow}SX$, where $S$ is a color-octet scalar, that is applicable to any NP model provided the dominant production mechanism is gluon-gluon fusion. The factorized result for the inclusive cross section is similar to that for the standard model Higgs production; however, differences arise due to color exchange between initial and final states. We provide formulas for the total cross section with large (partonic) threshold logarithms resummed to next-to-leading logarithm accuracy. The resulting $K$ factors are similar to those found in Higgs production. We apply our formalism to the Manohar-Wise model and find that the next-to-leading logarithm cross section is roughly 2 times (3 times) as large as the leading order cross section for a color-octet scalar of mass of 500 GeV (3 TeV). A similar enhancement should appear in any NP model with color-octet scalars.
Influence of environmental factors on broadleaf signalgrass (Brachiaria platyphylla) germination

Abstract Laboratory and greenhouse studies were conducted to determine the effect of temperature, solution pH, water stress, and planting depth on broadleaf signalgrass germination. Broadleaf signalgrass seed required removal of the husk for germination. When treated with constant temperature, broadleaf signalgrass germinated over a range of 20 to 35 C, with optimum germination occurring at 30 and 35 C. Onset, rate, and total germination (87%) was greatest in an alternating 20/30 C temperature regime. Germination decreased as solution pH increased, with greatest germination occurring at pH values of 4 and 5. Germination decreased with increasing water potential, and no germination occurred below − 0.8 mPa. Emergence was above 42% when seed were placed on the soil surface or buried 0.5 cm deep. Germination decreased with burial depth, but 10% of broadleaf signalgrass seed emerged from 6.0-cm depth. No seed emerged from 10-cm depth. These data suggest that broadleaf signalgrass may emerge later in the seaso...
An Electrochemical Study of Pyrrhotite-Grinding Media Interaction under Abrasive Conditions

Abstract The electrochemical nature of pyrrhotite-grinding media interaction was investigated by using an experimental setup in which an electrode was rotated against a porcelain ball immersed in a ground quartzite slurry. Galvanic currents obtained by superposition of the polarization curves of pyrrhotite and grinding media electrodes under abrasion were correlated with corrosion currents estimated by applying Faraday's law to the ball wear data of marked ball wear tests. The effect of abraded steel debris on corrosive wear in the grinding of a magnetite ore was also investigated.
Neonatal Diabetes Mellitus: The Impact of Molecular Diagnosis

Neonatal hyperglycemia has multiple causes, some of which are common (sepsis, stress, phenytoin or glucocorticoid administration) and others of which are more rare, including transient neonatal diabetes and mutations of sulfonylurea receptors, which require molecular diagnosis. Many infants identified with the latter condition may respond well to oral sulfonylurea medications. We describe an infant in whom molecular diagnosis permitted such therapy as well as a new diagnosis for the mother, who had been insulin-dependent for 17 years. In addition to aberrant expression of imprinted genes on chromosome 6q24 for transient neonatal diabetes, molecular diagnosis offers a rationale for oral hypoglycemic agents (off-label use) that may improve the lives of affected infants.
Doppler lidar measurements of winds and turbulence in the boundary layer

Coherent Doppler lidar is ideally suited for accurate measurements of the profiles of wind and turbulence using various beam scanning and processing techniques. The most attractive scanning pattern is a three dimensional pattern which samples the largest number of independent samples of the turbulent field. Careful processing of the radial velocity estimates is required to correct for the spatial filtering by the lidar pulse and to remove the bias introduced by the estimation error. The various processing techniques and fundamental assumptions will be presented. Lidar derived profiles of wind and turbulence will be compared with in situ data, when available. Results will also be presented from a field campaign focused on wind energy applications.
Pride, Prejudice, and a Dose of Shame: The Meaning of Public Assistance

Little is known about women’s retrospective views on the receipt of public assistance, including their relationships with their caseworkers. This article examines the findings of 20 in-depth interviews with women about their experiences with public assistance after they went from public assistance to a living-wage job. The respondents’ self-stories are interpreted using a social constructionist framework.
Demographic and socioeconomic aspects of elderly migration in the 1980s.

The availability of data from the Current Population Survey for each year makes it possible to monitor the migration behavior of the population on a year-to-year basis and facilitates efforts to detect changes in long-standing patterns of behavior. The data used in this article come from the published reports summarizing the results of the March administration of the Current Population Survey over a decade, and, in general, deal with the movement of persons during the 12-month interval immediately preceding the survey. Trends and changes in some of the principal aspccts of migration behavior among the elderly are reviewed and summarized, focusing on the demographic, social, geographic, and economic dimensions. Also considered are differences in migration behavior along these dimensions from a temporal perspective as well as in comparison to the migration behavior of the younger population. When possible, the article provides insight on behavioral differences among the various age categories within the old...
Optical imaging of apoptosis as a biomarker of tumor response to chemotherapy.

A rapid and accurate assessment of the antitumor efficacy of new therapeutic drugs could speed up drug discovery and improve clinical decision making. Based on the hypothesis that most effective antitumor agents induce apoptosis, we developed a near-infrared fluorescent (NIRF) annexin V to be used for optical sensing of tumor environments. To demonstrate probe specificity, we developed both an active (i.e., apoptosisrecognizing) and an inactive form of annexin V with very similar properties (to account for nonspecific tumor accumulation), and tested the agents in nude mice each bearing a cyclophosphamide (CPA) chemosensitive (LLC) and a chemoresistant LLC (CR-LLC). After injection with active annexin V, the tumor-annexin V ratio (TAR; tumor NIRF/background NIRF) for untreated mice was 1.22 ± 0.34 for LLC and 1.43 ± 0.53 for CR-LLC (n = 4). The LLC of CPA-treated mice had significant elevations of TAR (2.56 ± 0.29, P = .001, n = 4), but only a moderate increase was obtained for the CR-LLC (TAR = 1.89 ± 0.19, P = .183). The in vivo measurements correlated well with terminal deoxyribosyl transferasemediated dUTP nick end labeling indexes. When inactive Cy-annexin V was used, with or without CPA treatment and in both CCL and CR-CCL tumors, tumor NIRF values ranged from 0.91 to 1.17 (i.e., tumor were equal to background). We conclude that active Cy-annexin V and surface reflectance fluorescence imaging provide a nonradioactive, semiquantitative method of determining chemosensitivity in LLC xenografts. The method maybe used to image pharmacologic responses in other animal models and, potentially, may permit the clinical imaging of apoptosis with noninvasive or minimally invasive instrumentation.
The role of magnesium in muscle phosphorylase kinase activity

Abstract Phosphorylase kinase (ATP:phosphorylase phosphotransferase, EC 2.7.1.38), the enzyme which regulates glycogen breakdown by converting α-glucan phosphorylase (α-1,4-glucan:orthophosphate glucosyltransferase, EC 2.4.1.1) into its active form, requires ATP and divalent metal ions for its activity. We have shown that: 1. 1.MgATP 2−2 chelate is the substrate for the reaction with an apparent K m of 0.07 mM ± 0.02 (S.D.). 2. 2.Free Mg 2+ in mM concentration is also required for activity. The apparent K a for free Mg 2+ is 0.6 mM ± 0.2 (S.D.). 3. 3.Free ATP 4− is not inhibitory. 4. 4.Mn and Ca at mM concentrations are inhibitors of the enzyme even though μM concentrations of Ca are required for activity 1,2 . With both metals the inhibition is not competitive with respect to MgATP 2− and Mg 2+ . Mn as well as Al, Ba and Co, in the absence of Mg, will allow the phosphorylase kinase reaction to proceed but only at approx. 5% of the rate observed in the presence of Mg.
Computations of Pulsed Film-Cooling

Direct Numerical Simulation (DNS) of a pulsed film cooling jet is presented to examine if pulsations of the coolant jet can enhance film cooling effectiveness. Calculations are performed for a cylindrical jet inclined at 30-degrees. The jet pulsation is defined by the duty cycle (DC) and the Strouhal number (St), both of which are varied in the present study. Calculations are done for a baseline steady blowing ratio of 1.5. With a peak blowing ratio (M) of 1.5, pulsing with a St = 0.32 and DC = 0.5 is shown to reduce jet blow-off and improve centerline and spanwise-averaged effectiveness over the steady M = 1.5 case.Copyright © 2007 by ASME
Donkey Kong in little bear country: A first grader's composing development in the media spotlight

Young children may make extensive use of media texts (e.g., movies, cartoons, songs) in their storytelling and play. Their experience with such texts may become evident as children learn to use and produce written texts. Through their writing, media material, such as the video creature Donkey Kong, may mingle with school material such as the literary creature Little Bear. In this article, I examine how media use informs child composing by drawing on data collected in an ethnographic project in an urban first grade. I focus on the influence of visual media involving animation. By untangling the complexities of 1 child's case history, I illustrate how the media figure into the most basic processes of writing development, processes of differentiation of, translation across, and reframing within symbolic forms and social practices. I conclude with a consideration of the teaching challenges posed, and opportunities offered, by the children's media use.
Impacts of Rest‐Rotation Grazing on Stream Banks in Forested Watersheds in Idaho

Abstract Rest-rotation grazing in Idaho allowed forage in the stream-side zone to be used at a higher rate than on either immediately adjacent range or the overall grazing allotment. Stream sides received unauthorized grazing during the scheduled rest periods, however, and complete rest was difficult to achieve. Cattle appeared to graze stream-side meadows at high elevations with less intensity during the early grazing period when vegetation was lush than during the late grazing period. Stream-bank alteration occurred soon after cattle were turned into ungrazed meadows.
Bicuculline-pentobarbital interactions on [35S]TBPS binding in various brain areas.

Abstract The effect of in vitro addition of pentobarbital to brain membrane preparations from cerebellum and cortex of C57B1 mice was examined in the presence and absence of the specific GABAA receptor “antagonist” bicuculline. In the cortex pentobarbital produced a biphasic effect (stimulation followed by inhibition) on [35S]TBPS binding, whereas only inhibition of [35S]TBPS binding was observed in the cerebellum. When bicuculline was added to assay mixtures, the stimulatory action of pentobarbital was markedly enhanced in the cortex. In the cerebellum the presence of bicuculline uncovered a biphasic effect of pentobarbital on [35S]TBPS binding, that is lower doses of pentobarbital increased, while higher doses decreased [35S]TBPS to the membrane receptors from the cerebellum.
β-Adrenergic receptor signaling in the heart: Role of CaMKII

The multifunctional Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) targets a number of Ca(2+) homeostatic proteins and regulates gene transcription. Many of the substrates phosphorylated by CaMKII are also substrates for protein kinase A (PKA), the best known downstream effector of beta-adrenergic receptor (beta-AR) signaling. While PKA and CaMKII are conventionally considered to transduce signals through separate pathways, there is a body of evidence suggesting that CaMKII is activated in response to beta-AR stimulation and that some of the downstream effects of beta-AR stimulation are actually mediated by CaMKII. The signaling pathway through which beta-AR stimulation activates CaMKII, in parallel with or downstream of PKA, is not well-defined. This review considers the evidence for and mechanisms by which CaMKII is activated in response to beta-AR stimulation. In addition the potential role of CaMKII in beta-AR regulation of cardiac function is considered. Notably, although many CaMKII targets (e.g., phospholamban or the ryanodine receptor) are central to the regulation of Ca(2+) handling, and effects of CaMKII on Ca(2+) handling are detectable, inhibition or gene deletion of CaMKII has relatively little effect on the acute physiological contractile response to beta-AR. On the other hand CaMKII expression and activity are increased in heart failure, a pathophysiological condition characterized by chronic stimulation of cardiac beta-ARs. Blockade of beta-ARs is an accepted therapy for treatment of chronic heart failure although the rationale for its beneficial effects in cardiomyocytes is uncertain. There is growing evidence that inhibition or gene deletion of CaMKII also has a significant beneficial impact on the development of heart failure. The possibility that excessive beta-AR stimulation is detrimental because of its effects on CaMKII mediated Ca(2+) handling disturbances (e.g., ryanodine receptor phosphorylation and diastolic SR Ca(2+) leak) is an intriguing hypothesis that merits future consideration.
Intestinal T lymphocytes in the chicken express an integrin-like antigen

We report the characterization of a molecule recognized on chicken T cells by the murine A19 monoclonal antibody that was generated by immunization with intestinal intraepithelial lymphocytes. Immunofluorescence analysis indicated that both alpha beta and gamma delta T cell subpopulations in the intestine express the A19 antigen, but natural killer cells and B cells do not. The A19-marked T cells were preferentially localized in the intestinal epithelium and less frequently in the underlying lamina propria. T cells appearing in the intestine during embryonic life were A19 negative but acquired the antigen within the first few days after hatching. Although rarely found on cells in non-intestinal tissues at any age, very late expression of the A19 antigen could be induced by concanavalin A stimulation of splenic and circulating T cells. Transforming growth factor beta 1 enhanced this induction of A19 expression. The A19 molecules expressed by intestinal T cells and activated splenic T cells were biochemically identical, consisting of a multi-molecular complex of proteins with approximate M(r) of 205, 145 and 75 kDa under nonreducing conditions and 120, 90 and 28 kDa under reducing conditions. The characteristics of this multimolecular complex and its differential expression suggest that the A19 antigen is a member of the integrin family which may function in the retention of intestinal lymphocytes.
A Review of the Physical Properities of Liquid Ionization Chamber Media

We review those physical properties of liquid methane, argon, krypton, and xenon important in the design, construction, and operation of liquid ionization chambers. The thermodynamic properties, electrical and optical properties, effects of impurities, and atomic and nuclear properties are summarized.
Guided Waves in Moving Media

This paper contains a theoretical study of the guided waves in a moving isotropic medium. The normal modes which can exist in a circular or rectangular wave guides are found by solving the Maxwell-Minkowski Equations subject to the appropriate boundary conditions. By certain transformations of field vectors, it is possible to change the Maxwell-Minkowski Equations into familiar forms such that the method of vector potentials can be applied to derive complete expressions for the field vectors. The results demonstrate that expressions for the propagation constant and the transverse-wave impedance and admittance in stationary media are modified by terms independent of the guide geometry when the media are moving.
Tat-SIRT1 Tango

In a recent issue of Cell Host & Microbe, Kwon et al. (2008) report that the human immunodeficiency virus (HIV) transactivator Tat inhibits the SIRT1 deacetylase, resulting in increased acetylation of the NF-kappaB p65 subunit and subsequently in T cell hyperactivation.
Tracing specific synonymous codon-secondary structure correlations through evolution.

Abstract We previously showed that GAU codons are preferred (relative to synonymous GAC codons) for encoding aspartates specifically at the N-termini of α-helices in human, but not in E. coli, proteins. To test if this difference reflected a general difference between eucaryotes and procaryotes, we now extended the analysis to include the proteins and coding sequences of mammals, vertebrates, S. cerevisiae, and plants. We found that the GAU-α-helix correlation is also strong in non-human mammalian and vertebrate proteins but is much weaker or insignificant in S. cerevisiae and plants. The vertebrate correlations are of sufficient strength to enhance α-helix N-terminus prediction. Additional results, including the observation that the correlation is significantly enhanced when proteins that are known to be correctly expressed in recombinant procaryotic systems are excluded, suggest that the correlation is induced at the level of protein translation and folding and not at the nucleic acid level. To the best of our knowledge, it is not explicable by the canonical picture of protein expression and folding, suggesting the existence of a novel evolutionary selection mechanism. One possible explanation is that some α-helix N-terminal GAU codons may facilitate correct co-translational folding in vertebrates.
Anaerobic production of magnetite by a dissimilatory iron-reducing microorganism

The potential contribution of microbial metabolism to the magnetization of sediments has only recently been recognized. In the presence of oxygen, magnetotactic bacteria can form intracellular chains of magnetite while using oxygen or nitrate as the terminal electron acceptor for metabolism1. The production of ultrafine-grained magnetite by magnetotactic bacteria in surficial aerobic sediments may contribute significantly to the natural remanent magnetism of sediments2–4. However, recent studies on iron reduction in anaerobic sediments suggested that bacteria can also generate magnetite in the absence of oxygen5. We report here on a sediment organism, designated GS-15, which produces copious quantities of ultrafine-grained magnetite under anaerobic conditions. GS-15 is not magnetotactic, but reduces amorphic ferric oxide to extracellular magnetite during the reduction of ferric iron as the terminal electron acceptor for organic matter oxidation. This novel metabolism may be the mechanism for the formation of ultrafine-grained magnetite in anaerobic sediments, and couldaccount for the accumulation of magnetite in ancient iron formations and hydrocarbon deposits.
Ultrafiltration is theoretically equivalent to equilibrium dialysis but much simpler to carry out.

This study shows that if one component of a reaction at equilibrium is freely diffusible through a semipermeable membrane, and if an aliquot of this component is removed through the membrane at its equilibrium concentration, the concentration of this component in the reaction mixture remains unchanged. This is illustrated by the binding of manganese (Mn2+) to concanavalin A. It is also shown that, at concentrations of calcium ions near saturation levels (0.01 m CaCh2, pH 5.2, 0.2 m NaCl), the binding of 1 mol of Mn2+ is extremely strong, with a dissociation constant K < 10−7, and at least 1 additional mol of Mn2+/mol of concanavalin A binds less strongly. As the pH is lowered, the affinity decreases to a small extent, until at pH 1.82 approximately 0.25 mol of Mn2+ binds/mol of protein. A possible application of the method to measure binding as a function of ligand concentration is described.
High-risk Factors of Respiratory Distress Syndrome in Term Neonates: A Retrospective Case-control Study.

Background: Respiratory distress syndrome (RDS) is a common critical disease in term neonates, but reasons for the occurrence of RDS remains unclear. Aims: This study aimed to investigate the cause of RDS in full-term neonates by a retrospective case-control study. Study Design: Case-control study. Methods: Among the patients admitted to Bayi Children's Hospital between January 2008 and December 2010, a total of 205 full-term neonates with RDS were assigned to the study group, and 410 full-term neonates without RDS were assigned to the control group. Clinical information, including the presence or absence of premature rupture of membranes (PROM), gender of the neonates, mode of delivery, birth weight, and any conditions suffered by the neonates were recorded. Results: The results of logistic regression analysis showed that the following causes were closely correlated with term neonatal RDS: selective cesarean section (OR: 8.737; 95% CI: 5.232-14.588), severe birth asphyxia (OR: 6.988; 95% CI: 2.990-16.333), small gestational age (OR: 6.222; 95% CI: 2.001-8.993), maternal-fetal infection (OR: 5.337; 95% CI: 1.999-8.233), PROM (OR: 3.380; 95% CI: 1.986-5.754), male sex (OR: 2.641; 95% CI: 1.721-4.053), gestational glucose intolerance or diabetes (OR: 2.415; 95% CI:1.721-4.053), and low birth weight (OR: 2.323; 95% CI: 1.329-4.060). Conclusion: Several high-risk factors, such as selective cesarean section, severe birth asphyxia, maternal-fetal infection, PROM, and male sex are closely correlated with full-term neonatal RDS. These could provide a significant reference for the diagnosis and treatment of term neonatal RDS.
Imaging evaluation of implantation site of permanent direct His bundle pacing lead.

1547-5271/$-see front matter B 2014 Heart Rhythm Society. All rights reserved. the location of the DHBP lead with respect to the plane of the tricuspid valve (TV). We present a patient with DHBP and imaging visualization of the location of the pacing lead. A 42-year-old man with persistent atrial fibrillation, mildly reduced left ventricular function, and sinus and atrioventricular nodal dysfunction underwent a dual chamber pacemaker implantation using the Select Secure Medtronic 3830 ventricular lead with a Medtronic Select Site deflectable catheter (C304) by mapping the HB with unipolar recordings from the lead tip. DHBP was verified by showing that pacing produced an identical morphology of paced QRS to native QRS with an isoelectric interval between the pacing spike
Principal component analysis of Auger line shapes at solid—solid interfaces

Abstract The analysis of Auger line shape changes during a compositional depth profile can be used to determine the existence of a distinct chemical state at a solid interface. The procedure involves principal component analysis of a data matrix made up of selected Auger spectra from the depth profile. The number of chemical components with distinguishable Auger line shapes in the overlayer, interface region, and underlayer is equal to the number of non-zero eigenvalues in the data matrix. Because spectra contain noise, some criterion must be used to decide when an eigenvalue is statistically different from zero. Provided reference spectra of all chemical components are available, the eigenvector solution can be rotated to give quantitative information on each component. The procedures described in this work are more rigorous than the visual inspection of Auger line shapes and are less time consuming than spectral stripping or curve resolving procedures. Line shape analysis was applied to Cu MVV and Cu LMM Auger spectra from cuprous oxide heterojunction photocells. A layer of Cu metal was found to be present at the interface of a SnO2-doped CdO heterojunction photocell.
Multi-Hop Broadcasting in Vehicular Ad Hoc Networks with Shockwave Traffic

A primary goal of intelligent transportation systems (ITS) is to improve road safety. The ability for vehicles to communicate is a promising way to alleviate traffic accidents by reducing the response time associated with human reaction to nearby drivers. In addition the limitations of standard driving can be overcome by providing drivers with instantaneous information about complications up ahead. Shockwaves, induced by vehicle speed differentials, are a typical mobility pattern that occurs with the formation and propagation of vehicle queues. These induce sudden braking and increase the occurrence of traffic incidents. In this paper, we investigate safety applications in highways with shockwave mobility and different lane configurations in vehicular ad hoc networks (VANET). We evaluate the performance of multi-hop broadcast communication using the ns-2 simulator with vehicles following a shockwave mobility pattern in fully-connected traffic streams. We propose mechanism to improve broadcast reliability using dynamic transmission range that leverages our understanding of fundamental traffic flow relationships.
Success of chronic defibrillation and the role of antiarrhythmic drugs with the automatic implantable cardioverter/defibrillator.

Abstract Because the automatic internal cardioverter defibrillator's long-term ability to reduce arrhythmic mortality in patients with ventricular tachycardia/fibrillation is unknown, it is important to determine whether the threshold for defibrillation changes over time. Serial defibrillation thresholds were measured in 23 patients over a mean replacement time of 24.8 ± 7.5 months. In all cases the lead system was a superior vena cava coil to a left ventricular epicardial patch. The defibrillation threshold for the entire group increased from 12.3 ± 4.7 J to 16.9 ± 5.9 J (p
Adiponectin deficiency modulates adhesion molecules expression and cytokine production but does not affect disease severity in the transfer model of colitis.

We investigated the effect of adiponectin (APN) deficiency in the CD4+CD45RBhigh transfer model of colitis. Recombination activating gene (Rag)-1 knockout (KO) and Rag-1 APN KO mice receiving CD4+CD45RBhigh cells developed colitis of comparable severity. Colonic mRNA expression of IL-6 and IL-17 was lower in Rag-1 APN KO mice compared to Rag-1 KO mice. Rag-1 APN KO and Rag-1 KO mice released comparable amounts of IL-6 from colon cultures, whereas release of IL-17 was higher in Rag-1 APN KO compared to Rag-1 KO mice. Expression of TNFα mRNA was comparable in Rag-1 KO and Rag-1 APN KO mice, but protein release was lower in Rag-1 APN KO mice compared to Rag-1 KO mice. Levels of IFNγ and IL-10 at mRNA and protein were comparable in Rag-1 KO and Rag-1 APN KO mice. Higher mRNA expression of VCAM-1 was observed in the colon of healthy APN KO compared to WT mice, while induction of colitis resulted in a comparable increase in VCAM-1 expression in Rag-1 KO and Rag-1 APN KO mice. In conclusion, although APN regulates expression of cytokines and adhesion molecules in the colon, this does not result in alteration of overall colitis severity in the CD4+CD45RBhigh transfer model.
Potentiometric studies on yeast complex III.

Abstract Potentiometric measurements have been performed on Complex III from bakers' yeast. The midpoint potentials for the b and c cytochromes were measured using room-temperature MCD and liquid-helium temperature EPR. A value of 270 mV was obtained for cytochrome c 1 , regardless of temperature, while the midpoint potentials found for the two species of cytochrome b varied with temperatures, viz., 62 and −20 mV at room temperature (MCD) compared to 116 and −4 mV at about 10 K (EPR). The midpoint potential of the iron-sulfur center obtained by low-temperature EPR was 286 mV. An abrupt conformational change occurred immediately after this center was fully reduced resulting in a change in EPR line shape. The potentials of the two half-reactions of ubiquinone were measured by following the semiquinone radical signal at 110 K and 23°C. Potentials of 176 and 51 mV were found at low temperature, while values of 200 and 110 mV were observed at room temperature. The midpoint potential of cytochrome c 1 was found to be pH independent. The potentials of cytochrome b were also independent of pH when titrations were performed in deoxycholate buffers, while a variation of −30 mV per pH unit was observed for both cytochrome c species in taurocholate buffers. These two detergents also produced different MCD contributions of the two b cytochromes. A decrease in E m of greater than 300 mV was found in potentiometric measurements of cytochrome c 1 at high ratios of dye to Complex III. Antimycin does not affect the redox potentials of cytochrome c 1 but appears to induce a transition of the low-potential b heme to a high-potential species. This transition is mediated by ubiquinone.
Use Of The National Bureau Of Standards (NBS) Antireflective (AR)-Chromium Optical Linewidth Standard For Measurements On Other Types Of Chromium Photomasks

Both antireflective (AR)-chromium and bright-chromium photomasks are currently used in the production of integrated circuits. Differences in the optical transmittance and reflectance of these photomasks can significantly change the line-image threshold required for accurate edge detection in optical microscope linewidth measurements. The suitability of using a calibration curve based on an AR-chromium optical linewidth-measurement standard (SRM 474) from the National Bureau of Standards (NBS) to correct linewidth measurements on other types of photomasks is discussed. Linewidths on each of three chromium photomasks of different chromium thicknesses were measured on four different types of optical microscope linewidth measurement systems. These measurements were corrected using an SRM 474 and compared with measurements made on the NBS optical linewidth calibration system. For the two bright-chromium specimens with low transmittance, the residual differences between the corrected values and the NBS values as measured on the NBS calibration system are generally less than ±0.05 μm for three of the measurement systems. For the see-through AR-chromium photomask with a higher transmittance, the calibration curve does not correct all systematic errors greater than ±0.05 μm. These results support theoretical studies showing that the degree of correction for systematic linewidth errors varies with the transmittance of the chromium photomask being measured and with the measurement system.
Unconsciously Poisoning the Roots: Psychological Dynamics of the Bible in Jewish/Christian Conflict

The persistence of antisemitism in Christian cultures despite the irrationality and destructiveness of its myths and attitudes has led more than one writer to consider that it may be deeply rooted in psychological dynamics, both individual and social. Likewise, much has been written about how the New Testament writings, especially certain passages in the Gospels, reflect a bitter split between the emerging Christian community and other Jewish groups. Seldom, however, are these two avenues of exploration brought together, asking how psychological mechanisms of defense and the formation of the biblical texts interact. In a recent book, The Jew and Deicide: The Origin of an Archetype (Davis, 2003), psychologist Frederick B. Davis attempted to do just that. Unfortunately, his presentation is deeply flawed by his lack of familiarity with basic New Testament studies and methods. His fundamental premise is sound—that the interplay of the texts, the communities that created them, and their subsequent interpreters evidence psychological dynamics that fuel an ongoing conflict. In this paper, I will suggest how those dynamics were active within the first century Christian community and were expressed in New Testament writings, and how those same dynamics are stimulated in later readers.
Spider and Fly: The Leninist Philosophy of Georg Lukács

AbstractFrom 1919 to 1929, the great Hungarian Marxist philosopher Georg Lukacs was one of the leaders of the Hungarian Communist Party, immersed not simply in theorising but also in significant practical-political work. Along with labour leader Jeno Landler, he led a faction opposing an ultra-left sectarian orientation represented by Bela Kun (at that time also associated with Comintern chairman Zinoviev, later aligning himself with Stalin).If seen in connection with this factional struggle, key works of Lukacs in this period – History and Class Consciousness (1923), Lenin: A Study in the Unity of His Thought (1924), Tailism and the Dialectic (1926) and ‘The Blum Theses’ (1929) – can be seen as forming a consistent, coherent, sophisticated variant of Leninism. Influential readings of these works interpret them as being ultra-leftist or proto-Stalinist (or, in the case of ‘The Blum Theses’, an anticipation of the Popular Front perspectives adopted by the Communist International in 1935). Such readings distort the reality. Lukacs’s orientation and outlook of 1923–9 are, rather, more consistent with the orientation advanced by Lenin and Trotsky in the Third and Fourth Congresses of the Communist International.After his decisive political defeat, Lukacs concluded that it was necessary to renounce his distinctive political orientation, and completely abandon the terrain of practical revolutionary politics, if he hoped to remain inside the Communist movement. This he did, adapting to Stalinism and shifting his efforts to literary criticism and philosophy. But the theorisations connected to his revolutionary politics of the 1920s continue to have relevance for revolutionary activists of the twenty-first century.
Photoexcitation and Charge Transport in Organic Molecular Crystals

By using short wavelength tunable optical pulses to excite a transient photoconductivity and to write a transient photorefractive grating we uncover the unique mechanisms of photocarrier generation in Rubrene and of charge transport in DAST.
EEG features of the appalic syndrome resulting from cerebral anoxia

Three victims of severe cerebral anoxia developed the apallic syndrome, and clinical neurophysiological examinations documented slow evolution of a diurnal behavior pattern. Marked EEG attenuation was the primary finding, but all the patients exhibited clustering of quiescent periods at night, during which EEG waves were generated, and two stages suggested REM and non-REM sleep. The results seemed to confirm the notions that brain-stem mechanisms control occurrence of REM and non-REM sleep, and that forebrain mechanisms are required for development of differential EEG features of non-REM sleep stages (I through IV). It is also suggested that an extreme degree of attenuation resulting from EEG desynchronization in certain patients may closely resemble ECS.
Abstract B44: The epigenetic modifier CHAF1A opposes neuroblastoma differentiation via metabolic reprogramming

Background: Neuroblastoma arises from embryonal neural crest secondary to a block in differentiation and long-term survival inversely correlates with the degree of neuronal differentiation. Inhibition of epigenetically controlled developmental programs is critical for neuronal de-differentiation and neuroblastoma pathogenesis. Treatment with differentiation agents has modestly improved survival. Methods: Loss-of-function studies in neuroblastoma cell lines with inducible and stable CHAF1A knockdown were performed in vitro and in vivo. Gene expression profiling of knockdown and control cell lines was performed on the Affymetrix U133+2.0 arrays. GSEA (Gene Set Enrichment Analysis) defined the transcriptional response to CHAF1A silencing. Quantitative-PCR assays were used to correlate CHAF1A expression with outcome and to validate the most significant enriched gene sets. Results: Here we demonstrate a novel function for the histone chaperone and epigenetic regulator CHAF1A in maintaining the highly de-differentiated state of neuroblastoma. CHAF1A is a subunit of the Chromatin Assembly Factor-1 (CAF1) which regulates H3K9-trimethylation and DNA methylation. High CHAF1A expression strongly correlates with poor prognosis in a large clinical cohort of 348 neuroblastoma patients (Progression-Free and Overall Survival p Conclusions: Our findings support the hypothesis that CHAF1A expression restricts neural crest differentiation and contributes to the pathogenesis of high-risk neuroblastoma by epigenetic regulation of glucose metabolism. In particular, we believe that the oncogenic functions of CHAF1A in neuroblastoma in part direct the metabolic reprogramming, thus conferring cell survival advantages and blocking cell differentiation. Further understanding of the metabolic changes epigenetically induced by CHAF1A will identify vulnerable points to impair neuroblastoma cell growth and guide the development of novel therapies. Citation Format: Eveline Barbieri, Zaowen Chen, Anna Lakoma, Eugene Kim, Jason Shohet. The epigenetic modifier CHAF1A opposes neuroblastoma differentiation via metabolic reprogramming. [abstract]. In: Proceedings of the AACR Special Conference on Pediatric Cancer at the Crossroads: Translating Discovery into Improved Outcomes; Nov 3-6, 2013; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2013;74(20 Suppl):Abstract nr B44.
Minkowski Bessel modes.

The global Minkowski Bessel (MB) modes, whose explicit form allows the identification and description of the condensed vacuum state resulting from the operation of a pair of accelerated refrigerators, are introduced. They span the representation space of a unitary representation of the Poincar\'e group on two-dimensional Lorentz space-time. Their three essential properties are (1) they are unitarily related to the familiar Minkowski plane waves, (2) they form a unitary representation of the translation group on two-dimensional Minkowski space-time, and (3) they are eigenfunctions of Lorentz boosts around a given reference event. In addition the global Minkowski Mellin modes are introduced. They are the singular limit of the MB modes. This limit corresponds to the zero-transverse-momentum solutions to the zero-rest-mass wave equation. Also introduced are the four Rindler coordinate representatives of each global mode. Their normalization and density of states are exhibited in a (semi-infinite) accelerated frame with a finite bottom. In addition we exhibit the asymptotic limit as this bottom approaches the event horizon and thereby show how a mode sum approaches a mode integral as the frame becomes bottomless. This is the infinite Regge-Wheeler volume limit.
Tylden's Almanac: The Chapbook of a Royalist in the Restoration

Among Rutgers' extensive holding of almanacs is one of particular interest printed in 1663. But it is not so much the content of the almanac that is interesting but for the jottings of the owner of it in the almanac. The owner, "Willm Tylden" used the endpapers to copy down a variety of poems, ballads, epitaphs, epigraphs, and household accounts. His jottings make this old almanac a fascinating reflection of the turbulent years just after the Restoration, as well as a mirror of one man's ardent royalist sympathies and somewhat libertine tastes.
High speed detection of circulating tumor cells

Epithelial tumor cells circulate in peripheral blood at ultra-low concentrations in cancer patients. We have developed an instrument capable of rapid and accurate detection of rare cells in circulation utilizing fiber-optic array scanning technology (FAST). The FAST cytometer can locate immunofluorescently labeled rare cells on glass substrates at scan rates 500 times faster than conventional automated digital microscopy. These high scan rates are achieved by collecting fluorescent emissions using a fiber bundle with a large (50 mm) field of view. Very high scan rates make possible the ability to detect rare events without the requirement for an enrichment step. The FAST cytometer was used to detect, image and re-image circulating tumor cells in peripheral blood of breast cancer patients. This technology has the potential to serve as a clinically useful point-of-care diagnostic and a prognostic tool for cancer clinicians. The use of a fixed substrate permits the re-identification and re-staining of cells allowing for additional morphologic and biologic information to be obtained from previously collected and identified cells.
Ontogeny of α1- and β-adrenergic receptors in rat lung

Abstract The binding characteristics of the α 1 -selective adrenergic ligand [ 3 H]-prazosin were determined in particulate membranes of rat lung from day 18 of gestation to adulthood. Specific binding was present at all ages studied, was reversible and inhibition of specific binding by agonists followed the order of potency: (-)-epinephrine = (-)-norepinephrine >> (-)-isoproterenol > (+)-norepinephrine. Inhibition by antagonists followed the order of potency: prazosin > WB4101, >> yohimbine. Binding capacity increased during the neonatal period from 52±9 fmoles·mg −1 protein in lung preparations on day 18 of a 21 day gestation increasing to 105±4 fmoles·mg −1 protein (mean±SE) by postnatal day 15. Binding activity decreased thereafter, reaching adult levels by 28 days of postnatal age, 62±3 fmoles·mg −1 protein. This pattern of α 1 -adrenergic receptor density was distinct from that of β-adrenergic receptors identified in rat lung membrane with the β-adrenergic antagonist, (-)-[ 3 H]dihydroal-prenolol ((-)-[ 3 H]DHA). (-)-[ 3 H]DHA binding increased dramatically during this same time period, from 46±4 fmoles·mg −1 protein on day 18 of gestation to 496±44 fmoles·mg −1 protein in the adult lung. Affinity for [ 3 H]-prazosin and (-)-[ 3 H]DHA did not change with age. Pulmonary α 1 -adrenergic receptors are present as early as 18 days of gestation in the rat and α 1 -adrenergic receptor density is maximal by 15 days of postnatal age. The timing of the changes in α 1 -adrenergic receptors correlates with the timing of increased sympathetic innervation of the developing rat lung and is distinct from that of β-adrenergic receptor sites.
The stability of natural and synthetic neurohypophysial hormones in vitro.

The spontaneous decomposition of synthetic and natural neurohypophysial hormones in aqueous buffer solutions over a pH range of 3.0–8.5 at room temperature has been studied. Maximal stability for crude, highly purified and synthetic arginine vasopressin preparations was found at pH 3.0, 5.0 and 8.5. The decay proceeded most rapidly at pH 7.5 in phosphate buffer. The stability of arginine vasopressin at pH 7.5 was higher in diethylbarbiturate than phosphate buffer solution. Crude synthetic lysine vasopressin with low initial activity was most stable at pH 3 and 5. It was found to undergo less decomposition in phosphate buffer than the arginine congener. The highly purified and synthetic arginine vasopressin preparations underwent decomposition more rapidly than crude posterior pituitary extracts. The ratios of the antidiuretic, vasopressor and milk-ejection potencies of arginine vasopressin remained constant although the potencies of the solutions fell. The velocity of decomposition of crude natural and hi...
Using NanoDot dosimetry to study the RS 2000 X-ray biological irradiator

AbstractPurpose: To use NanoDot dosimeters to study the RS 2000 X-ray Biological Irradiator dosimetry characteristics and perform in vivo dosimetry for cell or small animal experiments.Methods and materials: We first calibrated the Landauer NanoDot™ Reader by irradiating some NanoDot dosimeters with a set of known doses at specific positions defined by the irradiator. A group of five NanoDot dosimeters were placed at five specific positions where the dose rates were known and provided by the irradiator. Each group was irradiated for a set of times respectively. By correlating the readings of dosimeters with the given irradiated doses, we established the dose-reading relationship for the irradiator under the specific running condition. The established calibration curve was validated by exposing arbitrary known doses to a set of dosimeters, using the Landauer NanoDot™ Reader to measure the doses, and then making the comparison between the two doses. To study the dose gradient of the X-ray inside the irradia...
ortho Substituent effect on a 1,5-H shift reaction during thermal decomposition of aryltriazenes

Abstract An ortho substituent group has a significant effect on thermal decomposition of aryltriazenes. When the ortho methoxy-substituted phenyltriazenes were treated with methyl iodide at 110–130 °C, 1,5-H shift products were obtained in fair to moderate yields.