cssi/SciSciGPT-SciSciNet huggingface dataset:
Model-guided design and characterization of a high-precision 3D printing process for carbohydrate glass

Abstract Water-soluble glass patterned by 3D printing is a versatile tool for tissue engineering and microfluidics. Glasses can be patterned layer-by-layer as in conventional fused deposition modeling but also along 3D, “freeform” paths. In the latter approach, extruding heated material through a nozzle translating in 3D space allows for fabrication of sparse, freestanding networks of cylindrical filaments. These freeform structures are suitable for sacrificial molding with a variety of media, leaving complex microchannel networks. However, 3D printing carbohydrate glass in this way presents several unique challenges: 1) the material must resist degradation and crystallization during printing, 2) the glass must be hot enough to flow freely during extrusion and fuse to the printed construct, while cooling rapidly to retain its shape upon exiting the nozzle, 3) the extruder needs to apply high pressure, with rapid stop and start times and 4) the net force that acts on the filament during extrusion must be minimized so that the filament shape is predictable, i.e., coincides with the path taken by the nozzle. First, we review the properties of commercially available carbohydrate glasses and provide a guide for processing isomalt, our material of choice, to achieve the best printing performance. A pressure-controlled, piston-driven extruder is then described which allows for rapid responses and precise control over the material flow rate. We then analyze the heat transfer within the filament and the forces that contribute to the filament’s final shape. We find that the dominant force is due to the radial flow of the molten glass as it exits the nozzle. This analysis is validated on a purpose-built isomalt 3D printer, which we utilize to characterize relationships between extrusion pressure, translation speed, filament diameter, and viscous force. The insights of the physics of the printing process enable fabrication of intricate freeform prints as well as layer-by-layer designs. The practical and theoretical considerations should facilitate adoption of additive manufacturing of carbohydrate glasses with applications to a wide variety of fields, including tissue engineering and microfluidics.
Patient-Provider Discussions About Strategies to Limit Air Pollution Exposures.

Introduction Exposure to air pollution negatively affects respiratory and cardiovascular health. The objective of this study was to describe the extent to which health professionals report talking about how to limit exposure to air pollution during periods of poor air quality with their at-risk patients. Methods In 2015, a total of 1,751 health professionals completed an online survey and reported whether they talk with their patients about limiting their exposure to air pollution. In 2017, these data were analyzed to assess the frequency that health professionals in primary care, pediatrics, obstetrics/gynecology, and nursing reported talking about limiting air pollution exposure with patients who have respiratory or cardiovascular diseases, were aged ≤18 years, were aged ≥65 years, or were pregnant women. Frequencies of positive responses were assessed across categories of provider- and practice-level characteristics. Results Overall, 714 (41%) respondents reported ever talking with their patients about limiting their exposure to air pollution. Thirty-four percent and 16% of providers specifically reported talking with their patients with respiratory or cardiovascular disease diagnoses, respectively. Percentages of health professionals who reported talking with their patients about limiting air pollution exposure were highest among respondents in pediatrics (56%) and lowest among respondents in obstetrics/gynecology (0%). Conclusions Despite the well-described health effects of exposure to air pollution, the majority of respondents did not report talking with their patients about limiting their exposure to air pollution. These findings reveal clear opportunities to improve awareness about strategies to limit air pollution exposure among sensitive groups of patients and their healthcare providers.
Techno-economic analysis of a megawatt-scale thermoplastic resin wind turbine blade

Abstract Two-part, in-situ reactive thermoplastic resin systems for composite wind turbine blades have the potential to lower the blade cost by decreasing cycle times, capital costs of both tooling and equipment, and energy consumption during manufacturing, and enabling recycling at the end of the blade life. This paper describes a techno-economic model used to estimate the cost of a thermoplastic wind turbine blade relative to a baseline thermoset epoxy blade. It was shown that a 61.5-m thermoplastic blade costs 4.7% less than an equivalent epoxy blade. Even though the thermoplastic resin is currently more expensive than epoxy, this cost reduction is primarily driven by the decreased capital costs, faster cycle times, and reduced energy requirements and labor costs. Although thermoplastic technology for resin infusion of wind turbine blades is relatively new, these results suggest that it is economically and technically feasible and warrants further research.
THE EFFECT OF TEST-WISENESS ON SELF-EFFICACY AND MATHEMATIC PERFORMANCE OF MIDDLE SCHOOL STUDENTS WITH LEARNING DISABILITIES

THE EFFECT OF TEST-WISENESS ON SELF-EFFICACY AND MATHEMATIC PERFORMANCE OF MIDDLE SCHOOL STUDENTS WITH LEARNING DISABILITIES By Phyllis L. M. Haynes, Ph.D. A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University. Virginia Commonwealth University, 2011 Co-Directors: John Kregel, Ed.D., Professor Department of Special Education and Disability Policy School of Education Dr. Colleen A. Thoma, Ph.D., Professor Department of Special Education and Disability Policy School of Education The purpose of this study was to investigate whether the Test-Taking Strategy would improve performance on math curriculum-based assessments of students with disabilities, and if students reported an increased sense of math self-efficacy as a result of learning the Test-Taking Strategy. The Test-Taking Strategy uses mnemonics to teach strategies to help students successfully navigate through assessments. This study used an experimental, single-subject, multiple-probe, multiple base-line design (Horner & Baer, 1978). The design featured multiple participants, and followed the design features of quantitative research (Horner & Baer, 1978, McMillan, 2004, & Mitchell & Jolley, 2004). The Test-Taking Strategy did result in improved performance on CBA (math quizzes) for some of the students in this study. However, some students did not increase performance on math CBA (math quizzes). Findings also indicated most students did not report an increased sense of math efficacy. Results of this study and the impact of these findings are discussed.
Analysis of the initial burst of drug release coupled with polymer surface degradation

Purpose. Local pH effect on the release of a model pH-inert hydrophobic drug coupled with polymer degradation is described at the induction phase of biodegradable polymer erosion for better understanding the nature of initial burst of a drug.
Production and mechanical characterization of free-standing pigmented paper coating layers with latex and starch as binder

Abstract The mechanical properties of paper coating layers are important in various converting processes such as printing and folding of the paper. A method was developed to produce free-standing pigmented coating layers thick enough to be tested in bending as well as tension. The mechanical properties of these coating layers were characterized. Free-standing films with two types of binder formulations, pure latex or latex-starch combinations, with different binder content were prepared through an innovative film forming method that allows uniform drying of the coating layer while minimizing cracking. Tensile and flexural samples were cut out of these films. Tensile and flexural tests were carried out on samples and the results were compared. Tensile and flexural moduli were found to be similar. However, both stress at failure and strain at failure were higher in flexural tests compared to those in tensile tests. Three different types of paper were coated with these formulations and their failure during printing was evaluated through a standard ink picking test. Papers with different basis weights and porosities were found to have different picking resistance values. An interesting result was that the picking resistance did not correlate with the elastic modulus of the coating, but with the strain at failure behavior. This result indicates that the stiffness of the coating layer is not as critical during printing compared to its flexibility.
Accounting for Spatial and Temporal Variability in Electric Power Model Point-Source Emissions

Air emissions from power plants can have a significant effect on air quality. However, models used to analyze and project trends in the energy system are not easily coupled with air quality simulation models. Most downscaling approaches use simplistic averaging and “growth in place” methods that do not account for future changes in the pattern of generation due to renewable penetration, nor for important variations in time and space in emissions that have important implications for air quality. For instance, emissions of NOx are strongly correlated with temperature, which is also correlated with conditions favorable for ozone (smog) episodes. This paper presents a new methodology for downscaling electric sector results from the USDOE National Energy Modeling System (NEMS) for input into air quality modeling systems. The downscaling methodology will be used to analyze air quality and resulting health impacts of energy sector transitions, including transportation electrification and increased use of natural gas and renewable sources.
Effect of Exercise and Aβ Protein Infusion on Long-Term Memory-Related Signaling Molecules in Hippocampal Areas.

Alzheimer’s disease (AD) results from over-production and aggregation of β-amyloid (Aβ) oligopeptides in the brain. The benefits of regular physical exercise are now recognized in a variety of disorders including AD. In order to understand the effect of exercise at the molecular level, we studied the impact of exercise on long-term memory-related signaling molecules in an AD rat model. The rat model of AD (AD rat) was produced by 14-day osmotic pump infusion of i.c.v. 250 pmol/day Aβ1–42. The effects of 4 weeks of regular rodent treadmill exercise on the protein levels of CREB, CaMKVI, and MAPK-ERK1/2 in this model were determined by immunoblot analysis in the CA1 and dentate gyrus (DG) areas of the hippocampus, which is among the first brain structures impacted by AD. Aβ infusion caused marked reductions in the basal protein levels of CaMKVI and phosphorylated CREB without significantly affecting total CREB levels in both CA1 and DG areas. As predicted, our exercise regimen totally prevented these effects in the brains of exercised AD rats. Surprisingly, however, neither Aβ infusion nor exercise had any significant effect on the levels of phosphorylated or total ERK in the CA1 and DG areas. Additionally, exercise did not increase any of these molecules in healthy normal rats, which indicated a protective effect of exercise. These findings suggest that CaMKIV is likely a major kinase for phosphorylation of CREB. Therefore, regular exercise is highly effective in preventing the effects of AD even at the molecular level in both areas of the hippocampus. Considering the well-known resistance of the DG area to insults relative to area CA1, the present findings revealed similar molecular vulnerability of the two areas to AD pathology.
Differences in overall survival for patients with cholangiocarcinoma: Racial/ethnic disparity or socioeconomic factors?

380Background: Inequities in cancer survival are well documented. Whether disparities in overall survival (OS) result from inherent racial differences in underlying disease biology or socioeconomic...
The management of cerebrospinal fluid shunt infections: a clinical experience.

Fifty patients with infected cerebrospinal fluid shunts were treated by one of three forms of treatment: a) Twenty-two patients had shunt removal, systemic antibiotic treatment, and either external ventricular drainage or intermittent ventricular taps for decompression and antibiotic administration. b) Seventeen patients had removal and immediate replacement of the shunt with intrashunt and systemic antibiotics. c) Eleven patients received intrashunt and systemic antibiotics without shunt removal.
Acoustically Detonated Biomolecules for Genetically Encodable Inertial Cavitation

Recent advances in molecular engineering and synthetic biology have made it possible for biomolecular and cell-based therapies to provide highly specific disease treatment. However, both the ability to spatially target the action of such therapies, and their range of effects on the target tissue remain limited. Here we show that biomolecules and cells can be engineered to deliver potent mechanical effects at specific locations inside the body under the direction of focused ultrasound. This capability is based on gas vesicles, a unique class of air-filled protein nanostructures derived from buoyant photosynthetic microbes. We show that low-frequency ultrasound can convert these nanoscale biomolecules into micron-scale cavitating bubbles, as demonstrated with acoustic measurements and ultrafast optical microscopy. This allows gas vesicles targeted to cell-surface receptors to serve as remotely detonated cell-killing agents. In addition, it allows cells genetically engineered to express gas vesicles to be triggered with ultrasound to lyse and release therapeutic payloads. We demonstrate these capabilities in vitro, in cellulo, and in vivo. This technology equips biomolecular and cellular therapeutics with unique capabilities for spatiotemporal control and mechanical action.
Learning Robust Manipulation Strategies with Multimodal State Transition Models and Recovery Heuristics

Robots are prone to making mistakes when performing manipulation tasks in unstructured environments. Robust policies are thus needed to not only avoid mistakes but also to recover from them. We propose a framework for increasing the robustness of contact-based manipulations by modeling the task structure and optimizing a policy for selecting skills and recovery skills. A multimodal state transition model is acquired based on the contact dynamics of the task and the observed transitions. A policy is then learned from the model using reinforcement learning. The policy is incrementally improved by expanding the action space by generating recovery skills with a heuristic. Evaluations on three simulated manipulation tasks demonstrate the effectiveness of the framework. The robot was able to complete the tasks despite multiple contact state changes and errors encountered, increasing the success rate averaged across the tasks from 70.0% to 95.3%.
Analysis of the gradient method with an Armijo–Wolfe line search on a class of non-smooth convex functions

It has long been known that the gradient (steepest descent) method may fail on non-smooth problems, but the examples that have appeared in the literature are either devised specifically to defeat a...
Correlations of HIV Preexposure Prophylaxis Indications and Uptake, Chicago, Illinois, 2015-2018.

Objectives. To examine gaps in identification of preexposure prophylaxis (PrEP) candidates, uptake, and use of PrEP by populations most likely to seroconvert.Methods. At a federally qualified healt...
Modeling the Tumor Microenvironment and Pathogenic Signaling in Bone Sarcoma

Investigations of cancer biology and screening of potential therapeutics for efficacy and safety begin in the preclinical laboratory setting. A staple of most basic research in cancer involves the ...
The impact of naturalistic cannabis use on self-reported opioid withdrawal

Abstract Objectives Four states have legalized medical cannabis for the purpose of treating opioid use disorder. It is unclear whether cannabinoids improve or exacerbate opioid withdrawal. A more thorough examination of cannabis and its impact on specific symptoms of opioid withdrawal is warranted. Method Two hundred individuals recruited through Amazon Mechanical Turk with past month opioid and cannabis use and experience of opioid withdrawal completed the survey. Participants indicated which opioid withdrawal symptoms improved or worsened with cannabis use and indicated the severity of their opioid withdrawal on days with and without cannabis. Results 62.5% (n = 125) of 200 participants had used cannabis to treat withdrawal. Participants most frequently indicated that cannabis improved: anxiety, tremors, and trouble sleeping. A minority of participants (6.0%, n = 12) indicated cannabis worsened opioid withdrawal, specifically symptoms of yawning, teary eyes, and runny nose. Across all symptoms, more participants indicated that symptoms improved with cannabis compared to those that indicated symptoms worsened with cannabis. Women reported greater relief from withdrawal with cannabis use than men. Discussion These results show that cannabis may improve opioid withdrawal symptoms and that the size of the effect is clinically meaningful. It is important to note that symptoms are exacerbated with cannabis in only a minority of individuals. Prospectively designed studies examining the impact of cannabis and cannabinoids on opioid withdrawal are warranted.
Adverse effects related to methotrexate polyglutamate levels: adjudicated results from the cardiovascular inflammation reduction trial.

Objectives Methotrexate is widely used at low dosages (LD-MTX) for non-oncologic indications and is associated with a variety of adverse effects (AEs). We sought to determine whether concentrations of the active metabolite, MTX polyglutamates (MTX-PGs) 1-5, correlate with AEs. Method We examined data from the LD-MTX arm of the randomized double-blind Cardiovascular Inflammation Reduction Trial (CIRT). All AEs were blindly adjudicated and monitoring laboratories were tested centrally. The MTX-PGs 1-5 were assessed in one reference laboratory using liquid chromatography-tandem mass spectrometry. Based on prior literature, MTX-PGs 3-5 were chosen as the exposure of interest and quartiles of MTX-PGs 3-5 were assessed for their relationship with all AEs and each pre-specified category of AE using adjusted Cox proportional hazards regression. Results Of the 2391 subjects randomized to LD-MTX, MTX-PG levels were available for 1319 subjects (median dosage 16.1 mg/week) from the 8 month visit. We followed these subjects for a median of 2.2 years [interquartile range (IQR) 1.5-2.9]. Higher MTX-PG3-5 levels were related to an increased risk of anaemia [compared with quartile 1 (Q1); hazard ratio (HR) for Q4 1.27 (95% CI 0.98, 1.65), P for trend = 0.05] and a decreased risk of thrombocytopenia [HR for Q4 0.52 (95% CI 0.32, 0.84), P for trend = 0.05]. MTX-PG3-5 levels >134 nmol/l were associated with an increased risk of liver abnormalities [HR 1.36 (95% CI 1.08, 1.72)]. Conclusions Higher MTX- PG3-5 levels were modestly associated with LD-MTX AEs, including anaemia and liver function abnormalities, but a reduced risk of thrombocytopenia and haemorrhage. Clinical trial registration NCT01594333.
The impact of adherence to a guideline for minimizing opioid use for treatment of pain in an urban emergency department.

Abstract Introduction The opioid epidemic has significantly evolved over the last three decades. The initiation and continuation of prescription opioids for pain control were one of the primary contributors, across different medical settings. The emergency department (ED) is typically the first place patients go to for management of acute pain, and often where opioid naive patients first become exposed to opioids. In 2018, the ED of University Hospital located in Newark, NJ implemented a pain guideline to ensure that patients are not unnecessarily exposed to opioids. The goal of our study was to determine whether provider adherence was successful in reducing opioid administration. Methods We conducted a retrospective review of pharmacy records of patients treated for pain in the ED within the time frame January 1, 2017 and December 31, 2019. We analyzed the change in our practice by comparing the amount of opioid and non-opioid medications administered and the number of patients administered each type, as well as the change in our utilization of specific medications. The t-test or the χ2 test were used as applicable. Results There were decreases in the mean number of opioid doses administered in 2017 (1273) compared to 2019 (498; p = 0.027). There was an increase in non-opioid analgesics administered, (mean 2017: 1817, mean 2019: 2432.5, p = 0.018). There was also an increase in the proportion of patients given non-opioid analgesics (mean 2017: 22%, mean 2019: 28%, p Discussion A guideline that emphasizes the use of non-opioid analgesics first line treatment for acute pain can be effective for reducing opioid administration in the ED. Through the use of our guideline, we reduced the number of patients who have received opioid analgesics and, at the same time, increased non-opioid analgesic administration. Future studies should explore readmission rates, duration of pain relief in patients managed with non-opioid versus opioid analgesics, and perception of relief through the use of satisfaction scores.
Extreme exoticism: Japan in the American musical imagination: by W. Anthony Sheppard, New York, Oxford University Press, 2019, 640 pp., $45 (hardback), ISBN: 9780190072704

With Extreme Exoticism: Japan in the American Musical Imagination, W. Anthony Sheppard brings into fruition twenty years of prodigious research on American responses to and representations of Japan...
Eco-martyrdom: Positive elements of self-sacrifice:

This theoretical article briefly explores the historical and modern concept of martyrdom and how it has taken on its current negative connotation due to suicide bombings after 9/11. The paper will ...
Willingness to lend resources is associated with increases in recovery and participation in community activities

Recovery homes may facilitate individuals with substance use disorders re-integration back into community settings by providing friendship, resources, and advice. Participants of the current study ...
Minimum-Weight Design of Stiffened Cylinders Under Hydrostatic Pressure

A methodology is developed by which one may design a stiffened cylinder of specified material, radius and length such that it can carry safely a given hydrostatic pressure which minimum weight. The solution is accomplished in two stages. First, design charts based on a simplified formulation of the objective function are obtained. Second, these design charts are used to evaluate the design variables. Such an approach enables the designer to introduce needed changes or avoid interaction of failure modes by paying the least weight penalty. Design examples are presented and the results are compared with those obtained by other investigators.
Why Modern Ethics Rejects the Common Good: Some Suggestions

We frequently hear that the concept of the common good has fallen out of favor in modern moral and political thinking in the West. It is a notion that has seemed to many to carry with it the teleological conception of nature and of the human person characteristic of the classical philosophy in which it finds its origin. Since the rejection of such teleological conceptions is among the deepest commitments of modern science, any political theory resting on these beliefs must also be rejected. Not only, however, is the common good tainted by its association with teleology, but it also has seemed to many to carry in its wake a theological taint associated with its prominent role in the political thought of the high Middle Ages, especially the thought of Thomas Aquinas. The modern liberal state is widely believed to have dispensed with the necessity for recourse to the common good. The citizens of a modern liberal state look for protection and for inspiration, not to some shared conception of the common good, identified and pursued by political leaders, but rather to a system of rights and liberties rooted more directly in the rationality and autonomy of individual citizens. The doctrines of (1) the priority of the right to the good and (2) neutrality with regard to the good (firmly anchored as they are at the heart of contemporary political theory) seem to leave little or no room for a rich notion of the common good. In addition, the widely held belief that “the fact of reasonable pluralism” is a central truth about social life under conditions of late modernity seems to call into question any sufficiently “common” feature of conceptions of the good to ground a robust notion of the common good.
Health Benefits and Wages: Minimizing the Total Cost of Compensation

We study the role of health benefits in an employer's compensation strategy, given the overall goal of minimizing the total compensation expense (wages plus health-insurance cost) for a fixed number of workers. The employer's basic benefit package consists of a base wage and a moderate health plan. It may also offer the option of upgrading to a generous health plan for an additional charge. Optimally, the base wage is set in order to balance the total wage bill against the expected cost of health care, while in setting the charge for generous coverage the employer acts as a monopolist who sells generous health plans to her employees. Seen from this perspective, extreme adverse selection and "premium death spiral" are not likely. However, the optimal compensation package may exclude either low-cost or high-cost employees.
Communicated Values as Informal Controls: Promoting Quality While Undermining Productivity?†

We find that the effectiveness of piece-rate compensation relative to fixed pay in a laboratory letter-search task hinges on the presence or absence of a nonbinding statement to participants that the experimenter values correct responses. In the absence of the value statement, participants with piece-rate rewards for correct responses generate more correct and incorrect responses than do their counterparts with fixed pay, correcting errors as they go along to maximize compensation. Essentially, piece-rate compensation acts as an output control, incentivizing participants to maximize correct responses through a “produce-and-improve” strategy. The value statement suppresses this strategy because participants appear to perceive it as an input constraint, prompting greater initial care at the expense of lower overall productivity. As a result, the value statement eliminates the gains in correct responses that piece-rate incentivized participants otherwise realize. Thus, in settings in which individuals can gain efficiency by working expeditiously and improving quality when necessary, our results suggest the possibility that organizations could be better off just letting incentive schemes operate, rather than emphasizing quality in ways that could overly constrain productivity.
Conversations about place: Dialogic ethics and land ethics in technical communication

This paper proposes a hybrid place-based ethic for technical communicators. In order to offer richer decisionmaking strategies that more fully consider modern concepts of the environment, and to address the need for humanistic technical communication, this paper offers a hybrid ethic based on Aldo Leopold's land ethic and Martin Buber's concept of the narrow ridge. In proposing this place-based ethic, we first describe Leopold's land ethic and Buber's narrow ridge. We then present brief analyses of case studies drawn from documents, and interviews conducted with practicing technical communicators to show applications for this approach.
Power controlled multiple access in ad hoc networks

This work presents PCMA, a power controlled multiple access wireless MAC protocol within the collision avoidance framework. PCMA generalizes the transmit-or-defer “on/off” collision avoidance model of current protocols to a more flexible “variable bounded power” collision suppression model. The algorithm is provisioned for ad hoc networks and does not require the presence of base stations to manage transmission power. Our initial simulation results show that PCMA can improve the throughput performance non-power controlled protocols by a factor of 2 with potential for additional scalability as source destination pairs become more localized.
Temporal Changes in State Transitions and Photosystem Organization in the Unicellular, Diazotrophic Cyanobacterium Cyanothece sp. ATCC 51142

The unicellular Cyanobacterium Cyanothece sp. ATCC 51142, grown under alternating 12-h light/12-h dark conditions, temporally separated N2 fixation from photosynthesis. The regulation of photosynthesis was studied using fluorescence spectra and kinetics to determine changes in state transitions and photosystem organization. The redox poise of the plastoquinone (PQ) pool appeared to be central to this regulation. Respiration supported N2 fixation by oxidizing carbohydrate granules, but reduced the PQ pool. This induced state 2 photosystem II monomers and lowered the capacity for O2 evolution. State 2 favored photosystem I trimers and cyclic electron transport, which could stimulate N2 fixation; the stimulation suggested an ATP limitation to N2 and CO2 fixation. The exhaustion of carbohydrate granules at around 6 h in the dark resulted in reduced respiratory electron flow, which led to a more oxidized PQ pool and produced a sharp transition from state 2 to state 1. This transient state 1 returned to state 2 in the remaining hours of darkness. In the light phase, photosystem II dimerization correlated with increased phycobilisome coupling to photosystem II (state 1) and increased rates of O2 evolution. However, dark adaptation did not guarantee state 2 and left photosystem I centers in a mostly monomeric state at certain times.
Dietary trilinoelaidate: effects on metabolic parameters related to EFA metabolism in rats.

To study the effects of dietary trilinoelaidate (ttl8:2) on metabolic parameters, as they relate to essential fatty acid metabolism, a diet containing "ade quate" linoleic acid with incremental isocaloric ttl8:2 supplementation was formulated. This diet was fed to rats for 11 weeks and oxygen (O2) consumption, insensible water loss (IWL), growth parameters and liver fatty acid composition evaluated at specified time intervals. The results showed that while an essential fatty acid deficient diet (hy- drogenated tallow) increased IWL and decreased body weight gains, Oj consumption remained unaltered. Dietary ttl8:2, while accumulating in liver lipids, and apparently inhibiting A6 desaturase, as evidenced by 18:2/20:4 ratios, did not alter O2 consumption or IWL. At the highest level of ttl8:2 fed (2.5 weight percent of dietary fat) there was a noticeable decrease in body weight gains by the llth week; organ weights, however, were not affected. While the increased consumption of dietary trans fatty acids has raised questions regarding their potential biologically detrimental effects, the present study indicated that gross metabolic alterations related to classical essential fatty acid deficiency symptoms are not precipitated in the rat by ttl8:2 levels comparable to those consumed in a normal human diet. J. Nutr. 112: 126-135, 1982.
Characterization of epitopes of human secretory component on free secretory component, secretory IgA, and membrane-associated secretory component

We have compared the epitopes present in various forms of human secretory component by using a panel of hybridoma-derived antibodies elicited by immunizing mice with free secretory component (FSC) or secretory IgA (sIgA). Enzyme-linked immunosorbent binding assays (ELISA) were used to assess antibody binding to FSC- and SC-containing antigens, including sIgA isolated from milk, reduced and alkylated sIgA, and sIgA assembled in vitro by incubating dimeric IgA with FSC. Immunofluorescence assays were also used to assess binding to a human epithelial tumor cell line (HT29) that expresses secretory component as an integral protein of the plasma membrane. The results can be summarized as follows. 1) Most antibodies from fusions in which sIgA was the immunizing antigen bound preferentially to sIgA. 2) Most antibodies from fusions in which FSC was the immunizing antigen bound preferentially to FSC. 3) Antibodies that bound preferentially to sIgA invariably bound sIgA assembled in vitro; antibodies that bound preferentially to FSC invariably did not. 4) Antibodies that bound readily to both sIgA and FSC were rare in all fusions. 5) The monoclonal antibodies defined at least six classes of epitopes on SC, including epitopes that were a) FSC specific and reduction sensitive, b) FSC specific and reduction insensitive, c) sIgA specific and reduction-sensitive, d) sIgA specific and reduction insensitive, e) shared by FSC and sIgA and reduction-sensitive, and f) shared by FSC and sIgA and reduction-insensitive. 6) Antibodies that mediated intense immunofluorescent staining of secretory component on HT29 cell membranes were rare and constituted a distinct subset of those which recognized epitopes shared by FSC, reduced and alkylated sIgA, and some preparations of native sIgA. Results of these antibody-binding studies indicate that most SC epitopes are not shared by FSC and sIgA. Most SC-related epitopes on sIgA appear to be generated by the physical interaction of SC with dimeric IgA, whereas most epitopes on FSC are masked or altered by this interaction. Finally, epitopes that are shared by membrane SC and FSC and/or sIgA represent a minor and immunochemically distinct subset of epitopes on SC. The high proportion of unique epitopes on the different physical forms of SC suggest that the epitopes of this molecule are highly sensitive to its molecular environment. The monoclonal reagents described here will be useful in studying the structure and function of SC; quantitating FSC, sIgA, and membrane SC; purifying various molecular forms of SC by immunoaffinity chromatography; and localizing SC in human tissues and cultured cells by immunocytochemical techniques.
FoxO6 Integrates Insulin Signaling With MTP for Regulating VLDL Production in the Liver

Excessive production of triglyceride-rich very low-density lipoproteins (VLDL-TG) contributes to hypertriglyceridemia in obesity and type 2 diabetes. To understand the underlying mechanism, we studied hepatic regulation of VLDL-TG production by (forkhead box O6) FoxO6, a forkhead transcription factor that integrates insulin signaling to hepatic metabolism. We showed that transgenic mice expressing a constitutively active FoxO6 allele developed hypertriglyceridemia, culminating in elevated VLDL-TG levels and impaired postprandial TG clearance. This effect resulted in part from increased hepatic VLDL-TG production. We recapitulated these findings in cultured HepG2 cells and human primary hepatocytes, demonstrating that FoxO6 promoted hepatic VLDL-TG secretion. This action correlated with the ability of FoxO6 to stimulate hepatic production of microsomal triglyceride transfer protein (MTP), a molecular chaperone that catalyzes the rate-limiting step in VLDL-TG assembly and secretion. FoxO6 was shown to bind to the MTP promoter and stimulate MTP promoter activity in HepG2 cells. This effect was inhibited by insulin, consistent with the ability of insulin to promote FoxO6 phosphorylation and disable FoxO6 DNA-binding activity. Mutations of the FoxO6 target site within the MTP promoter abrogated FoxO6-mediated induction of MTP promoter activity. Hepatic FoxO6 expression became deregulated in insulin-resistant mice with obesity and type 2 diabetes. FoxO6 inhibition in insulin-resistant liver suppressed hepatic MTP expression and curbed VLDL-TG overproduction, contributing to the amelioration of hypertriglyceridemia in obese and diabetic db/db mice. These results characterize FoxO6 as an important signaling molecule upstream of MTP for regulating hepatic VLDL-TG production.
Test-driven synthesis

Programming-by-example technologies empower end-users to create simple programs merely by providing input/output examples. Existing systems are designed around solvers specialized for a specific set of data types or domain-specific language (DSL). We present a program synthesizer which can be parameterized by an arbitrary DSL that may contain conditionals and loops and therefore is able to synthesize programs in any domain. In order to use our synthesizer, the user provides a sequence of increasingly sophisticated input/output examples along with an expert-written DSL definition. These two inputs correspond to the two key ideas that allow our synthesizer to work in arbitrary domains. First, we developed a novel iterative synthesis technique inspired by test-driven development---which also gives our technique the name of test-driven synthesis---where the input/output examples are consumed one at a time as the program is refined. Second, the DSL allows our system to take an efficient component-based approach to enumerating possible programs. We present applications of our synthesis methodology to end-user programming for transformations over strings, XML, and table layouts. We compare our synthesizer on these applications to state-of-the-art DSL-specific synthesizers as well to the general purpose synthesizer Sketch.
Identification of bromo/chloro dibenzo-dioxins and dibenzofurans in ash samples

Abstract This paper describes the analytical procedures, results and identification of bromo/chloro dibenzo- p -dioxins and dibenzofurans in ash samples from several types of combustion and incineration processes.
Consensus of best current management: The starting point for clinical quality assessment

Consensus of best current management developed by a rational and deliberative process can provide the basis for clinical quality assessment. Unfortunately, it is not always possible to arrive at a consensus at all cancer sites, and this generally indicates areas where clinical research is needed. Assessing the quality of care in these situations presents special problems. When it is possible to arrive at consensus in a specific disease, this consensus should detail appropriate pretreatment evaluation and the details of the treatment. Committees of experts for each specific disease site can formulate the consensus and must document their decisions based on information from the current world literature. A carefully thought out and documented consensus can then provide the basis for the development of process based questionnaires in assessing quality. We have observed that individuals formulating consensus of best current management do not strictly follow their own criteria, and that compliance in various strata of practice throughout the United States shows a greater deviation from consensus than anticipated and indeed this deviation crosses all types of practice. It was then necessary to conduct outcome surveys in the same patients to validate the processes of care by showing a correlation of process performance with outcome or indeed to change our concepts of best current management. We recognize from these outcome studies that relatively few processes have direct association with outcome and the majority of our consensus points relate to either good general patient management or items important to individual patients but not to large groups of patients. In addition to validating processes through outcome correlations, we have found that process verification is important. We have observed quite different outcomes for two groups of patients with Hodgkin's disease treated with the same processes (i.e., mantle field technology and adequate radiation dose, etc.). We were unable to identify the reason for an increased failure rate in one group of these patients until we looked at each individual mantle port film from the two groups of patients. We then identified that one facility was not including the Hodgkin's disease in the treatment portal due to poor technical performance. We believe that this program of process verification may be important in evaluating quality for any disease site. Data will be presented that illustrates the above problems.
The VAST Challenge: history, scope, and outcomes: An introduction to the Special Issue

The annual Visual Analytics Science and Technology (VAST) challenge provides Visual Analytics researchers, developers, and designers an opportunity to apply their best tools and techniques against ...
Soil temperature, physiology, and growth of containerized Annona species

Abstract The effects of soil temperature on physiology and growth of Annona glabra L. (pond apple) seedling trees, A. muricata L. (soursop) seedling trees, and A. squamosa L.× A . cherimola Mill. cv. Gefner (‘Gefner’ atemoya) nursery trees grafted on A. squamosa L. (sugar apple) rootstock in containers were investigated. Trees were exposed to soil temperatures of 5, 10, 20, 25 or 35 °C in controlled soil temperature chambers in a glasshouse. The leaf chlorophyll index was the lowest at 5 or 10 °C, and the highest at 35 °C for all species tested. Net CO 2 assimilation decreased to nearly zero within one week for each species exposed to 5 or 10 °C and became consistently negative over the remaining experimental period. The fresh and dry weights of roots, stems, and leaves decreased almost linearly as soil temperature decreased for ‘Gefner’ atemoya on sugar apple rootstock and soursop. There was a quadratic relationship between soil temperature and organ fresh and dry weights for pond apple, with the greatest fresh and dry weights occurring at soil temperatures of 25 °C. In general, reduced growth at lower temperatures was observed within 1 to 2 weeks after soil temperature treatments were initiated. Soursop and ‘Gefner’ atemoya on sugar apple rootstock are less tolerant of low soil temperatures than pond apple. Soursop and sugar apple were best adapted to soil temperatures of 25–35 °C and pond apple to 20–25 °C.
A Cleaved Amplified Polymorphic Sequence Marker to Detect Variation in Wx Locus Conditioning Translucent Endosperm in Rice

The translucent endosperm trait in a japonica rice variety 'Kantou 194' is controlled by a Wx-mq gene which is allelic to Wx locus by genetic analysis and allelic test. The amylose content analysis showed that an intermediate amylose content between those of glutinous and non-glutinous rice existed in endosperm of homozygous Wx-mq genotype. The slight changes of amylose content in different varieties and F1 grains with an identical Wx-mq genotype might be influenced by dissimilar genetic background. To identify the Wx-mq genotype simply and rapidly, a cleaved amplified polymorphic sequence (CAPS) marker was designed. The result from the molecular detection indicated that it could be used for marker-assisted selection for low amylose content varieties in rice breeding.
What Barriers Thwart Postpartum Women’s Physical Activity Goals During a 12-Month Intervention? A Process Evaluation of the Nā Mikimiki Project

Approximately 70% of new mothers do not meet national guidelines for moderate-to-vigorous physical activity (MVPA). The Nā Mikimiki ("the active ones") Project (2008-2011) was designed to increase MVPA among women with infants 2-12 months old. Participants' barriers to exercising and achievement of specific MVPA goals were discussed during telephone counseling calls over 12 months. Healthy, inactive women (n = 115, mean age = 31 ± 5 years, infants' mean age = 5.5 ± 3 months; 80% racial/ethnic minorities) received a total of 17 calls over 12 months in three phases. During Phase 1 weekly calls were made for a month, in Phase 2 biweekly calls were made for 2 months, and in Phase 3 monthly calls were made for 9 months. Across all phases, the most frequent barriers to achieving MVPA goals were: time/too busy (25%), sick child (11%), and illness (10%). Goals for MVPA minutes per week were achieved or surpassed 40.6% of the time during weekly calls, 39.9% during biweekly calls, and 42.0% during monthly calls. The least likely MVPA goals to be achieved (p < 0.04) were those which the woman encountered and for which she failed to overcome the barriers she had previously anticipated would impair her improvement of MVPA. This process evaluation demonstrated that telephone counseling somewhat facilitated the resolution of barriers and achievement of MVPA goals; thus, if clinical settings adopted such methods, chronic disease risks could be reduced in this vulnerable population of new mothers.
The H19 long noncoding RNA gives rise to microRNAs miR-675-3p and miR-675-5p to promote skeletal muscle differentiation and regeneration

Regulated expression of the H19 long noncoding RNA gene has been well characterized as a paradigm for genomic imprinting, but the H19 RNA's biological function remains largely unclear. H19 is abundantly expressed maternally in embryonic tissues but is strongly repressed after birth, and significant transcription persists only in skeletal muscle. Thus, we examined the role of the H19 RNA in skeletal muscle differentiation and regeneration. Knockdown of H19 RNA in myoblast cells and H19 knockout mouse satellite cells decreases differentiation. H19 exon1 encodes two conserved microRNAs, miR-675-3p and miR-675-5p, both of which are induced during skeletal muscle differentiation. The inhibition of myogenesis by H19 depletion during myoblast differentiation is rescued by exogenous expression of miR-675-3p and miR-675-5p. H19-deficient mice display abnormal skeletal muscle regeneration after injury, which is rectified by reintroduction of miR-675-3p and miR-675-5p. miR-675-3p and miR-675-5p function by directly targeting and down-regulating the anti-differentiation Smad transcription factors critical for the bone morphogenetic protein (BMP) pathway and the DNA replication initiation factor Cdc6. Therefore, the H19 long noncoding RNA has a critical trans-regulatory function in skeletal muscle differentiation and regeneration that is mediated by the microRNAs encoded within H19.
Hydrogen bonding constrains free radical reaction dynamics at serine and threonine residues in peptides.

Free radical-initiated peptide sequencing (FRIPS) mass spectrometry derives advantage from the introduction of highly selective low-energy dissociation pathways in target peptides. An acetyl radical, formed at the peptide N-terminus via collisional activation and subsequent dissociation of a covalently attached radical precursor, abstracts a hydrogen atom from diverse sites on the peptide, yielding sequence information through backbone cleavage as well as side-chain loss. Unique free-radical-initiated dissociation pathways observed at serine and threonine residues lead to cleavage of the neighboring N-terminal Cα–C or N–Cα bond rather than the typical Cα–C bond cleavage observed with other amino acids. These reactions were investigated by FRIPS of model peptides of the form AARAAAXAA, where X is the amino acid of interest. In combination with density functional theory (DFT) calculations, the experiments indicate the strong influence of hydrogen bonding at serine or threonine on the observed free radical c...