cssi/SciSciGPT-SciSciNet huggingface dataset:
[SWI+], the Prion Formed by the Chromatin Remodeling Factor Swi1, Is Highly Sensitive to Alterations in Hsp70 Chaperone System Activity

The yeast prion [SWI+], formed of heritable amyloid aggregates of the Swi1 protein, results in a partial loss of function of the SWI/SNF chromatin-remodeling complex, required for the regulation of a diverse set of genes. Our genetic analysis revealed that [SWI+] propagation is highly dependent upon the action of members of the Hsp70 molecular chaperone system, specifically the Hsp70 Ssa, two of its J-protein co-chaperones, Sis1 and Ydj1, and the nucleotide exchange factors of the Hsp110 family (Sse1/2). Notably, while all yeast prions tested thus far require Sis1, [SWI+] is the only one known to require the activity of Ydj1, the most abundant J-protein in yeast. The C-terminal region of Ydj1, which contains the client protein interaction domain, is required for [SWI+] propagation. However, Ydj1 is not unique in this regard, as another, closely related J-protein, Apj1, can substitute for it when expressed at a level approaching that of Ydj1. While dependent upon Ydj1 and Sis1 for propagation, [SWI+] is also highly sensitive to overexpression of both J-proteins. However, this increased prion-loss requires only the highly conserved 70 amino acid J-domain, which serves to stimulate the ATPase activity of Hsp70 and thus to stabilize its interaction with client protein. Overexpression of the J-domain from Sis1, Ydj1, or Apj1 is sufficient to destabilize [SWI+]. In addition, [SWI+] is lost upon overexpression of Sse nucleotide exchange factors, which act to destabilize Hsp70's interaction with client proteins. Given the plethora of genes affected by the activity of the SWI/SNF chromatin-remodeling complex, it is possible that this sensitivity of [SWI+] to the activity of Hsp70 chaperone machinery may serve a regulatory role, keeping this prion in an easily-lost, meta-stable state. Such sensitivity may provide a means to reach an optimal balance of phenotypic diversity within a cell population to better adapt to stressful environments.
Light microscopy of the exfoliation syndrome

The exfoliation syndrome affects all structures of the ocular anterior segment, as well as the conjunctiva and occasionally, nonocular structures. The exfoliative material has been shown by a series of light microscopic and gross anatomic studies to be only loosely adherent to the anterior lens capsule, zonules and anterior vitreous face, and firmly adherent to the equatorial lens capsule and posterior epithelium of the iris and the nonpigmented ciliary epithelium. Electron microscopy demonstrates that, in these latter regions, exfoliation material consisting of characteristic, cross-banded fibrils embedded in an amorphous matrix, is present both within the epithelial cells and associated with a disorganized, reduplicated basement membrane. These findings suggest that the material arises from the epithelium of the lens, iris and ciliary body, possibly the result of an underlying metabolic disorder. From these areas, the material enters the aqueous humor and later deposits on the anterior lens capsule, zonules, vitreous face, anterior surface of the iris, and trabecular meshwork. Histochemical studies demonstrate the presence of glycosaminoglycans, which may comprise the interfibrillar portion of the exfoliative material. Other studies demonstrate histochemical similarities between exfoliative material and zonules and are supported by recent work suggesting that the exfoliative fibrils are related to the microfibrillar portion of elastin. Although some reports suggest similarities between exfoliative material and amyloid, a majority of histochemical studies do not support this possibility.
Global ischemia induces NMDA receptor-mediated c-fos expression in neurons resistant to injury in gerbil hippocampus

The induction of c-fos protein-like immunoreactivity (CFPLI) was examined in the hippocampus of gerbils at several time points after transient global ischemia. c-Fos protein induction was largely confined to the dentate gyrus, CA3 and CA4 regions from 2 to 8 h after transient bilateral carotid occlusion. Little CFPLI was seen in the CA1 subfield, which is disproportionately sensitive to injury after global ischemia. c-Fos induction was completely blocked by pretreatment with MK-801 (3 mg/kg). Our results show that c-fos expression after global ischemia is NMDA receptor mediated, and mainly found in hippocampal neurons resistant to ischemic injury.
Adult muscle ‘stem’ cells can be sustained in culture as free-floating myospheres

The effectiveness of cell-based therapy to treat muscle disease has been hampered by difficulties in isolating, maintaining and propagating the stem cells that are needed for treatment. Here we report the isolation of muscle-derived stem cells from both young and old mice and their propagation over extended periods of time in culture as "free-floating" myospheres. Analysis of these sphere-forming cells showed that they express stem cell antigen-1 (Sca-1), beta1 integrin (CD29), Thy-1 (CD90), and CD34, but did not express CD45, CD31, or myogenic markers (Pax7, Myf5, and MyoD). We found that cells derived from myospheres and then grown adherently (MDACs) behaved similar to primary myoblasts, in that these cells expressed myogenic markers and were able to easily form multinucleated myotubes. Unlike the parental myospheres but analogous to primary myoblasts, MDACs expressed Pax7, Myf5, and MyoD, indicating that the parent myosphere cells were a more primitive type of cell. In support of this we demonstrated that myospheres were also able to differentiate into adipogenic and osteogenic cells in culture, as well as being able to contribute to injured muscle in vivo. In summary, we report that primitive adult muscle stem cells can be easily isolated and sustained in culture as myospheres.
Individual differences in reappraisal ability: Links to reappraisal frequency, well-being, and cognitive control

Reappraisal is generally viewed as an adaptive emotion regulation strategy. Reappraisal frequency has been associated with greater well-being, and reappraisal ability is thought to be composed of several crucial cognitive control processes. However, the relationships among reappraisal ability, reappraisal frequency, well-being, and various cognitive control processes have not yet been determined. In this study, we experimentally examined individual differences in reappraisal ability (RA), and also assessed reappraisal frequency, well-being, and several cognitive control processes. We observed a positive relationship between RA, reappraisal frequency, and well-being. RA was also related positively to working memory capacity and set-shifting costs, and marginally related to abstract reasoning. These findings have important implications for understanding the cognitive components and affective outcomes of RA.
Service Use Patterns for Adolescents with ADHD and Comorbid Conduct Disorder

Service use patterns and costs of youth diagnosed with attention-deficit/hyperactivity disorder (ADHD) and comorbid conduct disorder (CD) were assessed across adolescence (ages 12 through 17). Featured service sectors include mental health, school services, and the juvenile justice system. Data are provided by three cohorts from the Fast Track evaluation and are based on parent report. Diagnostic groups are identified through a structured assessment. Results show that public costs for youth with ADHD exceed $40,000 per child on average over a 6-year period, more than doubling service expenditures for a non-ADHD group. Public costs for children with comorbid ADHD and CD double the costs of those with ADHD alone. Varying patterns by service sector, diagnosis, and across time indicate different needs for youth with different conditions and at different ages and can provide important information for prevention and treatment researchers.
Nonpulsatile and Noninvasive Transmittance and Reflectance Tissue-bed Oximetry

A new optical device was developed that measures blood pressure noninvasively, in small human subjects (neonates and premature infants) and small animals (Roeder RAR. Transducer for indirect measurement of blood pressure in small human subjects and animals, Purdue University, BME; 2003.: xi, 50 p.). The ability of this device to measure oxygen saturation enhances its value. The objective of this research was to add the ability to obtain SaO(2) from the same device and to obtain the calibration curve. Another objective was to determine which measurement method (transmittance or reflectance) is preferable. This new oximeter is unlike the conventional pulse oximeter in that it does not require a pulse, making it ideal for measuring oxygen saturation noninvasively in small human subjects with small amplitude pulses or without a pulse. A study was performed in 11 pigs, ranging in weight 20-27 kg. The pig tail was used as the measuring site for %SaO(2) measurements. Arterial blood samples were obtained from the femoral artery and oxygen saturation was measured with a blood-gas analyzer. A small blood-pressure cuff was used to render the optical path bloodless. A comparison of the transmittance and reflectance methods for measuring oxygen saturation was made. %SaO(2) measurements ranged from 4% to 100%. It was found that both the transmittance and reflectance methods can be used to measure %SaO(2) reliably in situations with or without a pulse.
Insects as chemosensors of humans and crops

Insects transmit disease to hundreds of millions of people a year, and cause enormous losses to the world's agricultural output. Many insects find the human or plant hosts on which they feed, and identify and locate their mates, primarily through olfaction and taste. Major advances have recently been made in understanding insect chemosensation at the molecular and cellular levels. These advances have provided new opportunities to control insects that cause massive damage to health and agriculture across the world.
Supplementable Adequacy: Ground for a Situated Certainty

L'A. definit l'evidence adequate comme le critere de la certitude absolue chez Husserl. Examinant l'objection de Derrida et D. M. Levin concernant le caractere situe de la connaissance, l'A. defend la disponsibilite de l'adequation, malgre l'experience immanente et l'orientation transcendante de la phenomenologie.
The pathophysiology of ocular allergy : current thinking

Seasonal allergic conjunctivitis is the only ocular disease to involve solely Type-1 hypersensitivity, the other main forms of ocular allergy--perennial allergic conjunctivitis, vernal and atopic keratoconjunctivitis and giant papillary conjunctivitis--each having a more complex immunological basis and a chronic inflammatory component. Involvement of secondary inflammatory cells, particularly eosinophils, in addition to the mast cells resident in the conjunctival substantia propria, can lead to more serious corneal damage with vision-threatening potential. Thoughtful management of allergic conjunctivitis is needed in order to control the ocular inflammation without incurring steroid-induced side-effects, and patient education is also an important factor in maintaining optimal allergen avoidance, especially in the more severe and chronic cases. Laboratory models can be helpful in assessing the potential of new drugs, and SWR mice (topically sensitised and challenged with short ragweed) show clinical signs of allergic conjunctivitis, together with mast cell and eosinophil involvement, remarkably similar to the human pathophysiology. The antiinflammatory activity of both steroids and nedocromil sodium observed in this animal model supports therapeutic evidence of the usefulness of second-generation mast cell stabilising drugs in the treatment of ocular allergy.
Managing the Stigma of Personal Bankruptcy

Abstract In recent years, the relationship between personal bankruptcy and stigma has generated much discussion and speculation. However, virtually no research has examined the experiences of bankrupt debtors themselves. In this paper, we provide an analysis of bankruptcy stigma based on thirty-seven in-depth interviews with 19 married couples who filed for personal bankruptcy in 1999. The data demonstrate that stigma is a pervasive feature of contemporary personal bankruptcy and that bankrupt debtors rely upon a wide range of stigma management techniques employed by other stigmatized groups. We conclude by considering the implications of our findings for continued research on personal bankruptcy in the U.S. and for understanding, more broadly, the strategies and struggles for social agency among stressed and marginalized populations.
Ovarian aromatase cytochrome P-450 mRNA levels correlate with enzyme activity and serum estradiol levels in anestrous, pregnant and lactating rats.

We examined the changes in P-450AROM mRNA, aromatase enzyme activity and serum estradiol levels (E2) in anestrous, pregnant mare's serum gonadotropin (PMSG)-treated immature, pregnant, and lactating rats to determine if: (a) the various mRNA species encoding P-450AROM in rat ovarian tissue are differentially expressed during different hormonal states, and (b) a positive relationship exists between P-450AROM mRNA and enzymatic activity in rat ovarian tissue and serum estradiol levels from the same animals. Utilizing three different cDNAs encoding rat P-450AROM, levels of P-450AROM mRNA were determined by RNA blot analysis and scanning densitometry. Probe 1, a 5' probe, detects all three P-450AROM mRNA species in rat ovarian tissue (i.e. at 1.7, 2.2 and 2.7 kb). Probe 2 contains an unspliced intronic sequence in place of the heme-binding domain at its 3' terminus and thus the mRNA detected by this probe must encode a nonfunctional aromatase protein. Only the two smaller (i.e. nonfunctional) mRNA species at 1.7 and 2.2 kb are detected by probe 2. Probe 3 contains the heme-binding region and hybridizes to principally the largest mRNA transcript at 2.7 kb (but hybridizes also to a 1.7 kb mRNA transcript). Aromatase enzyme activity was measured by using a saturating concentration of [1 beta-3H]testosterone as substrate in the [3H]water-release assay while serum estradiol levels were determined by radioimmunoassay. In immature rats (IR) or lactating animals (LA) P-450AROM mRNA was not detectable along with low serum estradiol (IR approximately 2.8 pg/ml; LA approximately 0.2 pg/ml) and aromatase activity levels (IR approximately 0.8 pmol/h per mg protein; LA less than 0.5 pmol/h per mg protein). Anestrous animals treated with 5 IU of PMSG resulted in a clear increase (24 h later) in P-450AROM mRNA levels, in concert with a 4-fold increase in serum E2 (approximately 12.5 pg/ml) and aromatase activity (approximately 4.2 pmol/h per mg protein). During pregnancy, all three mRNA species were clearly detectable, but low serum E2 levels (approximately 0.6 pmol/ml) and P-450AROM mRNA abundance were observed at 3 days of gestation (DG). Levels of all three P-450AROM mRNA species increased markedly at 15 and 18 DG; thereafter, the levels declined at 20 DG and further decreased at 22 DG. However, regardless of the probe utilized (probe 1, 2 or 3) in the RNA blot analyses, the mRNA transcripts detected by each probe were expressed in a concerted fashion with respect to abundance and pattern.(ABSTRACT TRUNCATED AT 400 WORDS)
Optical properties of silicon clusters deposited on the basal plane of graphite

Laser ablation was used to deposit of silicon on highly oriented pyrolytic graphite surfaces in an ultra high-vacuum environment equipped with Auger electron spectroscopy (AES), scanning tunneling microscopy (STM) and luminescence spectroscopy. For deposition of up to several monolayers, post annealing produced silicon clusters, whose size distribution was determined vs annealing time and temperature using STM. Pure silicon clusters ranging from 1 to 10 nm showed no detectable photoluminescence in visible range. Exposure to oxygen at 10{sup {minus}6} Torr and for up to 8 hours showed adsorption on the surface of the clusters without silicon oxide formation and no detectable luminescence. Hydrogen termination of these clusters was accomplished by exposing them to atomic hydrogen beam but did not result in any photoluminescence. Prolonged exposure of these clusters to ambient air, however, resulted in strong photoluminescence spectra with color ranging from red to greenish-blue depending on average cluster size. Auger electron spectra revealed the existence of partially oxidized silicon clusters. This luminescence could be due to either an oxide phase or to changes in electronic structure of the clusters as a result of quantum confinement effect.
Desensitization of G-protein-coupled receptors: Agonist-induced phosphorylation of the chemoattractant receptor cAR1 lowers its intrinsic affinity for cAMP

Agonist-induced phosphorylation of G-protein-coupled receptors has been shown to facilitate the desensitization processes, such as receptor internalization, decreased efficiency of coupling to G-proteins, or decreased ligand affinity. The lowered affinity may be an intrinsic property of the phosphorylated receptor or it may be the result of altered interactions between the modified receptor and downstream components such as G-proteins or arrestins. To address this issue, we purified cAR1, the major chemoattractant receptor of Dictyostelium discoideum by a strategy that is independent of the ligand binding capacity of the receptor. To our knowledge, this represents the first successful purification of a chemoattractant receptor. The hexyl-histidine-tagged receptor was solubilized from a highly enriched plasma membrane preparation and purified by Ni2+-chelating chromatography. The protocol offers a simple way to purify 100-500 micrograms of a G-protein coupled receptor that can be targeted to the plasma membrane of D. discoideum. The Kd value for the purified cAR1 was about 200 nM, consistent with that of receptors that are not coupled to G-proteins in intact cells. In contrast, the affinity of phosphorylated cAR1, purified from desensitized cells, was about three times lower. Treatment of the phosphorylated receptor with protein phosphatases caused dephosphorylation and parallel restoration of higher affinity. We propose that ligand-induced phosphorylation of G-protein-coupled receptors causes a decrease in intrinsic affinity and may be useful in maintaining the receptor's sensitivity at high agonist levels. This affinity decrease may precede other processes such as receptor internalization or uncoupling from G-proteins.
Image contrast enhancement in compressed wavelet domain

This paper presents a new and efficient framework for image contrast and color enhancement in the compressed wavelet domain such as JPEG 2000. The proposed approach is capable of improving both global and local contrast as well as preserving color consistency. The framework does not require inverse transform for image enhancement since linear scale factors are directly applied to both scaling and wavelet coefficients in the compressed domain, which results in high computational efficiency. Also contaminated noise in the image can be efficiently reduced by introducing wavelet shrinkage terms adaptively in the different scales. Experimental results show that the proposed method can enhance wavelet-coded image computationally efficiently with high image quality and less noise or other artifact.
Alpha Adrenergic Regulation of Celiac Blood Flow and Plasma Catecholamine Response During Acute Heat Stress in Fed Cockerels

Hubbard cockerels with chronically implanted electromagnetic blood flow probes on the celiac artery were used to establish a relationship between changes in postprandial celiac mean blood flow (MBF) and plasma catecholamines during a acute heat exposure. Five min after the elevation of ambient temperature from 25 to 37 C, there were concomitant reductions (P less than .05) in celiac MBF, norepinephrine (NE), and heart rate (HR). After 50 min of heat stress, rectal temperature (Tr), respiratory rate (RR), plasma epinephrine (E), and celiac vascular resistance (CVR) were significantly greater (P less than .05) than preheat stress thermoneutral control values. During the thermoneutral recovery period, all parameters returned to values comparable to preheat exposure control with the exception of NE, which tended (P less than .1) to remain lower. To determine the role of the sympathetic nervous system in regulating postprandial celiac MBF during acute heat exposure, chronically instrumented cockerels were infused with phenoxybenzamine, an alpha-adrenergic receptor-blocking agent. Alpha-receptor blockade attenuated both postprandial intestinal hyperemia under thermoneutral conditions as well as the heat-induced reduction of postprandial celiac MBF. The results of these studies implicate the sympathetic nervous system in the regulation of postprandial celiac MBF in heat-stressed cockerels and indicate a possible alpha-adrenergic-mediated mechanism involved in postprandial intestinal hyperemia.
Dynamic Surface Tensions of Aqueous Surfactant Mixtures: Experimental Investigation

We present an experimental investigation of the interfacial behavior of aqueous solutions of two nonionic surfactants and their binary mixture. Specifically, we measure both the equilibrium surface tensions (using the Wilhelmy plate method) and the dynamic surface tensions (using the pendant bubble technique) of aqueous solutions of two nonionic alkyl ethoxylate surfactants, dodecyl penta(ethylene oxide) (C12E5) and decyl octa(ethylene oxide) (C10E8), as well as of their binary mixture. These measurements are then compared with the predictions made using recently developed molecular-thermodynamic theories of the equilibrium and the dynamic interfacial behaviors of nonionic surfactant mixtures and are found to be in good agreement. The main advantage of the equilibrium and the dynamic interfacial theories adopted here is that they are based on the molecular characteristics of the surfactants, and as such, the required number of experimentally determined parameters can be reduced significantly. Indeed, no e...
Functionality of Varroa-Resistant Honey Bees (Hymenoptera: Apidae) When Used for Western U.S. Honey Production and Almond Pollination

Two types of honey bees, Apis mellifera L., bred for resistance to Varroa destructor Anderson & Trueman, were evaluated for performance when used for honey production in Montana, and for almond pollination the following winter. Colonies of Russian honey bees and outcrossed honey bees with Varroa-sensitive hygiene (VSH) were compared with control colonies of Italian honey bees. All colonies were managed without miticide treatments. In total, 185 and 175 colonies were established for trials in 2010–2011 and 2011–2012, respectively. Survival of colonies with original queens or with supersedure queens was similar among stocks for both years. Colony sizes of the Varroa-resistant stocks were as large as or larger than the control colonies during periods critical to honey production and almond pollination. Honey production varied among stocks. In the first year, all stocks produced similar amounts of honey. In the second year, Russian honey bees colonies produced less honey than the control colonies. V. destructor infestations also varied among stocks. In the first year, control colonies had more infesting mites than either of the Varroa-resistant stocks, especially later in the year. In the second year, the control and outcrossed Varroa-sensitive hygiene colonies had high and damaging levels of infestation while the Russian honey bees colonies maintained lower levels of infestation. Infestations of Acarapis woodi (Rennie) were generally infrequent and low. All the stocks had similarly high Nosema ceranae infections in the spring and following winter of both years. Overall, the two Varroa-resistant stocks functioned adequately in this model beekeeping system.
A two-stage classifier system for normal mammogram identification

In this paper, we present a unique two-stage classifier system for identifying normal mammograms. We present methods that extract features from breast regions characterizing normal and cancerous tissue. A subset of the features is used to construct a classifier. This classifier is then used to classify each mammogram as normal or abnormal. We designed a unique two-stage cascading classifier system. A binary decision tree classifier was used in the first stage. It is designed to correctly classify cancerous regions. The regions classified as abnormal in the first-stage were used as input to the second-stage classifier, a linear classifier. We will show that the overall performance of our two-stage cascading classifier is better than a single classifier. Results for full-field normal mammogram analysis using this classifier are comparable to a human reader.
The development and validation of the state and trait food-cravings questionnaires

We describe three studies on the development and psychometric properties of state and trait versions of a multidimensional Food Cravings Questionnaire (FCQ-S and FCQ-T). In Study 1, we used confirmatory factor analysis to help refine the instruments. Results indicated good internal consistency for both questionnaires and their respective subscales, as well as excellent test-retest reliability for the FCQ-T. In Study 2, we examined the validity of the instruments by comparing the effect of food deprivation versus food satiation on both questionnaires. As hypothesized, we found a greater effect on the state measure than the trait measure. In Study 3, we used confirmatory factor analysis to cross-validate the factor structure of both questionnaires with a new sample. Results supported the factor structure of both instruments. Overall, we suggest that cravings can be conceptualized as multidimensional motivational states. Our preliminary data support the use of the FCQ-S and FCQ-T with clinical and nonclinical populations.
Earth-Moon Mass Ratio from Mariner 9 Radio Tracking Data

The phase-coherent range and Doppler tracking data obtained as a basis for the navigation of the Mariner 9 spacecraft from earth to Mars determine also the earth-moon mass ratio. As the earth revolves about the center of mass of the earth-moon system, a sinusoidal curve is impressed on the range and Doppler tracking data with a frequency equal to the sidereal mean motion of the moon. The mass ratio was determined from range and Doppler data obtained over a period of 15 weeks. The results from the Mariner Mars 1971 data are presented in a table together with previous results obtained in connection with other spacecraft.
Interexaminer agreement in the measurement of periodontal disease.

Six variables of periodontal pathology were independently measured by two periodontists in a study of healthy male adult participants from the VA Dental Longitudinal Study. Two examination sets containing a total of 50 volunteers were compared to establish interexaminer agreement. Measures of gingival recession, tooth mobility, calculus accumulation, and plaque accumulation exhibited high interexaminer correlation. The measures of periodontal pocket depth and gingival health also showed good levels of agreement, but reflected the examination order; the second examiner consistently scored more severe disease because of the invasive nature of these measures. Establishment of the reproducibility of periodontal pathology measurements is essential for studies which involve more than one examiner. This investigation demonstrated a high confidence level of reproducibility for the six measures utilized and showed that recalibration enhanced the initial training procedure and increased examiner agreement above the initial level.
Ultrasound-Guided Needle Localization during Open Parotid Sialolithotomy.

ObjectivesOur objective is to describe a novel operative technique for localization of parotid sialolithiasis, demonstrate the feasibility of the technique, and discuss its indications.Study DesignProspective study.SettingTertiary-level academic center.Subjects and MethodsPatients with symptomatic parotid sialolithiasis who had failed prior sialendoscopic extraction underwent ultrasound needle localization and open sialolithotomy. Data were prospectively collected. Independent variables included size of and location of sialoliths.ResultsEleven patients were treated using transcutaneous ultrasound-guided needle placement and injection of methylene blue prior to external sialolithotomy. Follow-up ranged from 6 to 12 months. Ten (91.9%) patients had stones within the proximal one-third of the ductal lumen, and 1 (9.1%) had stones present within both the proximal one-third and middle one-third of the ductal lumen. The average surgical time was 53 ± 10.8 minutes. The average sialolith length was 7.6 ± 2 mm. Th...
A Monte Carlo method for statistical derivatives of arbitrary spectra

The usual method of obtaining statistical derivatives such as significant heights, average periods etc., from sea or response spectra is to assume a particular probability distribution function and then calculate the derivatives from the properties of the assumed distribution. A method of generating time-domain records that satisfy a target spectra density and then calculating the statistical derivatives directly from these records is presented in this paper.
Familial short stature caused by haploinsufficiency of the insulin-like growth factor i receptor due to nonsense-mediated messenger ribonucleic acid decay.

Background: IGF-I, essential for normal human growth in utero and postnatally, mediates its effects through the IGF-I receptor (IGF1R), a widely expressed, cell surface tyrosine kinase receptor. Five cases of heterozygous mutations in the IGF1R gene have been identified in patients with varying degrees of intrauterine and postnatal growth retardation. Objective: The objective of the study was the analysis of the IGF1R gene in a short-statured patient and his affected family members. Patient: The male patient, with a height of −3.1 sd score (SDS; aged 12 yr), had normal circulating levels of GH binding protein, IGF-I, and IGF binding protein-3. His mother (−4.6 SDS), one of his siblings (−1.94 SDS), and several other maternal family members were also short statured. Results: The patient, his mother, and the short-statured sibling carry a novel heterozygous 19-nucleotide duplication within exon 18 of the IGF1R gene, which introduces a premature termination codon at codon 1106 of the IGF1R open reading frame...
Response of mouse tissues to neutron and gamma radiation: protection by WR-3689 and WR-77913.

The response of mouse intestine to d(22 MeV) + Be neutrons, p(50.5 MeV) + Be neutrons, or 137Cs γ-rays was examined using intestinal crypt-cell survival as an endpoint. The RBEs for 50.5 MeV neutrons relative to 137Cs-γ-rays of response levels of 5 and 50 surviving cells/circumference were 1.80 and 1.86. For d(22 MeV) + Be neutrons, the RBEs at comparable cell survivals were 1.86 and 1.93. For LD507 day (gastrointestinal syndrome), the RBE for 50.5 MeV neutrons was 1.56 and for LD5030 (bone marrow syndrome), the RBE was 1.29. The ability of the phosphorothioates WR-3689 [S-2-(3-methylaminopropylamino) ethylphosphorothioic acid, 450 mg/kg] and WR-77913 [S-2-(3-amino-2-hydroxypropyl) phosphorothioic acid, 800 mg/kg] to protect against p(50.5 MeV) + Be neutrons and γ-rays also was examined in mice using lethality at seven or 30 days as the endpoints. Results were compared to previous studies with d(22 MeV) + Be neutrons WR-2721 (400 mg/kg), an appropriate comparison given the similar RBEs of the two neutron sources relative to γ-rays. WR-77913 is a less effective protector than is WR-2721 while in some cases WR-3689 is as effective as WR-2721. The neutron DMFs for LD507 were 1.12 for WR-3689 and 0.99 for WR-77913. For the bone marrow syndrome (LD5030) the neutron DMFs were 1.47 for WR-3689 and 1.21 for WR-77913. For a given type of radiation, WR-3689 is more effective than WR-77913. Both phosphorothioates protect less well against neutron radiation than against photons, as was reported previously for WR-2721. Protective factors are higher for bone marrow than for gut. Our results differ from other investigators who have reported that some phosphorothioates protect better against fission neutrons than against X- or γ-rays.
Overview of distal myopathies: from the clinical to the molecular

Five distinct predominantly distal myopathies have been identified with discrete clinical and genetic patterns as follows: (1) Welander myopathy (late adult onset, type 1), with autosomal dominant inheritance and unknown molecular localization; (2) Markesbery-Griggs/Udd myopathies (late adult onset, type 2), with autosomal dominant inheritance and linkage to chromosome 2q; (3) Nonaka myopathy (early adult onset, type 1), with autosomal recessive inheritance and molecular localization to 9p1-q1. Nonaka myopathy is identical to quadriceps-sparing familial inclusion body myopathy; (4) Miyoshi myopathy (early adult onset, type 2), with autosomal recessive inheritance and localization to 2p; (5) Laing myopathy (early onset, type 3), with autosomal dominant inheritance and linkage to chromosome 14. The gene and abnormal gene product have not yet been defined for any of the distal myopathies. However, it is already clear that disorders allelic to the distal myopathies can begin with proximal weakness. Given such major phenotypic variation, it is possible that some of the diseases we regard as distal myopathies may become obsolete. Instead, these conditions may become known by their genetic mutation or abnormal gene product, much like Duchenne and Becker dystrophy.
HER-2/neu expression in primary and metastatic breast cancer

Introduction Trastuzumab is a highly effective therapy for the treatment of HER-2/neu positive breast cancer. To maximize benefit and minimize unnecessary toxicity, patient selection is essential. Currently HER-2/neu analysis is routinely performed only for primary invasive breast cancers, and trastuzumab therapy is recommended based on primary analysis only. Methods Using immunohistochemistry, we performed a retrospective study comparing HER-2/neu expression in original primary to subsequent metastatic breast cancers. Results Tumors from 382 patients with metastatic breast cancer were studied. In 254 cases (66%) both primary and metastatic lesions were concordant. In 90 cases the primary lesion was HER-2/neu positive with the metastatic lesion negative; whereas, in 37 cases the primary lesion was HER-2/neu negative and the metastatic lesion positive. Primary HER-2/neu immunostaining was associated with a negative predictive value of 35.7%. Although all four groups were similar at diagnosis, survival differences were noted with the best survival experienced by patients with initial primary lesions HER-2/neu negative and subsequent metastatic lesions positive. Patients with hormone receptor and HER-2/neu positive primary lesions who received tamoxifen were more likely to have HER-2/neu positive metastasis. Conclusions The significant discordance between HER-2/neu expression in primary and metastatic tumors suggests that determination of HER-2/neu status in metastatic disease should be attempted.
When your eyes see more than you do

Visual information is used by the brain to construct a conscious experience of the visual world and to guide motor actions [1]. Here we report a study of how eye movements and perception relate to each other. We compared the ability of human observers to perceive image motion with the reliability of their eyes to track the motion of a target [2], [3] and [4], the goal being to test whether both motor and sensory processes are based on the same set of signals and limited by a shared source of noise [2] and [4]. We found that the oculomotor system can detect fluctuations in the velocity of a moving target better than the observer. Surprisingly, in some conditions, eye movements reliably respond to the velocity fluctuations of a moving target that are otherwise perceptually invisible to the subjects. The implication is that visual motion signals exist in the brain that can be used to guide motor actions without evoking a perceptual outcome nor being accessible to conscious scrutiny.
DISKLIKE STRUCTURE IN THE SEMIREGULAR PULSATING STAR X HERCULIS

This paper reports the results of a Berkeley-Illinois-Maryland (BIMA) array interferometric observation of a semiregular pulsating star with an unusual narrow molecular line profile, X Her, in the CO J = 1-0 line. In the CO spectrum, a double-component profile (including narrow and broad components) is seen, as reported by previous observations. The narrow component consists of two spiky peaks. The spatial structure of the board component shows bipolar shape, and that of the narrow component shows an elliptical/spherical shape. The two peaks in the narrow component show a systematic difference in the integrated intensity map. The kinematical and geometrical properties of the narrow component are reminiscent of a Keplerian rotating disk with a central mass of 0.9 M☉, although an interpretation as an expansion disk seems to be more natural. A secondary bipolar flow instead of the disk also cannot be fully excluded as an interpretation of the narrow line.
Genetic and phenotypic analysis of reassortants of high growth and low growth strains of influenza B virus

Abstract The yield of influenza virus in eggs is critical to influenza vaccine production and availability, but the contribution of specific genes to the growth properties of influenza B viruses is not well understood. Influenza B/Beijing/184/93 and B/Shangdong/7/97 were chosen for study because B/Shangdong/7/97 replicated to several fold higher titers in eggs than B/Beijing/184/93 as demonstrated by hemagglutination titers and EID 50 . A reassortant with the HA , NP and PB2 genes from B/Beijing/184/93 and all other genes from B/Shangdong/7/97 had the high growth phenotype of B/Shangdong/7/97 in eggs, which suggests that NS , M , NA , PB1 or PA , or a combination of these genes derived from B/Shangdong/7/97were needed for the high growth phenotype of the reassortants. A high degree of homology was found among the genetic sequences of B/Beijing/184/93, B/Shangdong/7/97, and other influenza B viruses. However, differences potentially related to growth characteristics were suggested by analysis of the deduced amino acid (AA) sequences of four genes: NS ( NS1 , NS2 ), M ( BM2 ), NA ( NA , NB ) and PB1 . The studies identify multiple genes that may affect growth of influenza B viruses in eggs.
Flapping dynamics of a flag in a uniform stream

We consider the flapping stability and response of a thin two-dimensional flag of high extensional rigidity and low bending rigidity. The three relevant non-dimensional parameters governing the problem are the structure-to-fluid mass ratio, μ = ρ s h /(ρ f L); the Reynolds number, Re=VL/ν; and the non-dimensional bending rigidity, K B = EI / (ρfV 2 L 3 ). The soft cloth of a flag is represented by very low bending rigidity and the subsequent dominance of flow-induced tension as the main structural restoring force. We first perform linear analysis to help understand the relevant mechanisms of the problem and guide the computational investigation. To study the nonlinear stability and response, we develop a fluid-structure direct simulation (FSDS) capability, coupling a direct numerical simulation of the Navier-Stokes equations to a solver for thin-membrane dynamics of arbitrarily large motion. With the flow grid fitted to the structural boundary, external forcing to the structure is calculated from the boundary fluid dynamics. Using a systematic series of FSDS runs, we pursue a detailed analysis of the response as a function of mass ratio for the case of very low bending rigidity (K B = to-4) and relatively high Reynolds number (Re=10 3 ). We discover three distinct regimes of response as a function of mass ratio μ: (I) a small μ regime of fixed-point stability; (II) an intermediate μ regime of period-one limit-cycle flapping with amplitude increasing with increasing μ; and (III) a large μ regime of chaotic flapping. Parametric stability dependencies predicted by the linear analysis are confirmed by the nonlinear FSDS, and hysteresis in stability is explained with a nonlinear softening spring model. The chaotic flapping response shows up as a breaking of the limit cycle by inclusion of the 3/2 superharmonic. This occurs as the increased flapping amplitude yields a flapping Strouhal number (St=2Af/V) in the neighbourhood of the natural vortex wake Strouhal number, St ≃ 0.2. The limit-cycle von Karman vortex wake transitions in chaos to a wake with clusters of higher intensity vortices. For the largest mass ratios, strong vortex pairs are distributed away from the wake centreline during intermittent violent snapping events, characterized by rapid changes in tension and dynamic buckling.
Hamiltonian formulation of quantum error correction and correlated noise: Effects of syndrome extraction in the long-time limit

We analyze the long time behavior of a quantum computer running a quantum error correction (QEC) code in the presence of a correlated environment. Starting from a Hamiltonian formulation of realistic noise models, and assuming that QEC is indeed possible, we find formal expre ssions for the probability of a given syndrome history and the associated residual decoherence encoded in the reduced density matrix. Systems with non-zero gate times (“long gates”) are included in our analysis by using an upper bound on the noise. In order to introduce the local error probability for a qubit, we assume that propagation of signals through the environment is slower than the QEC period (hypercube assumption). This allows an explicit calculation in the case of a generalized spin-boson model and a quantum frustration model. The key result is a dimensional criterion: If the correlations decay sufficiently fast, the system evolves toward a stochas tic error model for which the threshold theorem of fault-tolerant quantum computation has been proven. On the other hand, if the correlations decay slowly, the traditional proof of this threshold theorem does not hold. T his dimensional criterion bears many similarities to criteria that occur in the theory of quantum phase transitio ns.
The potential role of excessive cortisol induced by HPA hyperfunction in the pathogenesis of depression

Prolonged hypothalamic-pituitary-adrenocortical (HPA) axis overactivity occurs at all levels of this axis during stress in normals and some depressed patients. This can induce enlargement of the pituitary and adrenals. Various reports showed that cortisol can affect mood and behavior, and disrupt memory and recall. The integrity of the hippocampus is essential for memory function and, via the high density of its cortisol receptors, cortisol induced inhibitory feedback to the HPA axis. Animal data suggest that over time aging and stress can permanently downregulate hippocampal cell receptors, produce chronic hippocampal inflammation (astroglial), and kill cells. Cushing's syndrome patients (high cortisol) show diminished hippocampal size and verbal recall inversely related to cortisol levels. All the above is consistent with the 'cascade hypothesis' of cortisol induced hippocampal damage with resultant diminished inhibition to HPA hyperactivity in a circular manner. High cortisol is associated with altered neurotransmitter function, e.g., diminished brain serotonin synthesis, low CSF 5HIAA, and increased noradrenergic activity.
The effects of talc pleurodesis on growing swine

Abstract Talc pleurodesis in an effective means of preventing recurrent pneumothorax. We have successfully applied talc pleurodesis with thoracoscopy in children with cystic fibrosis presenting with pneumothorax. However, little is known about the effects of talc pleurodesis on lung compliance in growing children. Therefore, six young pigs (10 weeks old, weighing 15 ± 1 kg) were prepared for study. In each pig, one hemithorax underwent thoracoscopy and talc pleurodesis (TALC). The other hemithorax underwent either thoracoscopy alone or no procedure (CONTROL). Dynamic and static respiratory mechanics were studied 5 and 10 weeks later. Air flow and airway pressure were measured to calculate dynamic transpulmonary and transrespiratory compliance, and static transpulmonary compliance. At 5 weeks, dynamic transpulmonary and transrespiratory compliance were less in the TALC lungs when compared with CONTROL lungs. At 10 weeks, the differences in dynamic transpulmonary and transrespiratory compliance between the TALC and CONTROL lungs had resolved. Static compliance was lower in the TALC lungs than in the CONTROL lungs at both 5 and 10 weeks, but this was significant only at 10 weeks. There was an improvement in static compliance in the TALC lungs between 5 and 10 weeks, but this only approached significance. At autopsy, there were marked adhesions and pleural thickening in the talc lungs. Histological examination demonstrated no differences in lung parenchyma between the TALC lungs and the CONTROL lungs. Talc pleurodesis causes a temporary impairment in dynamic transpulmonary and transrespiratory compliance that resolves with time and growth. Static compliance is more persistently compromised, but a trend toward improvement with time and growth exists. Talc pleurodesis does not affect the lung parenchyma. These data indicate that talc pleurodesis has minimal long-term effects on pulmonary compliance in growing pigs, and are consistent with clinical findings of the safety of talc pleurodesis in humans.
A simple device for washing large numbers of sections and tissues.

A simple, efficient, and inexpensive device which washes up to 480 slides simultaneously, is described. It consists essentially of a deeper lower tray to hold the slides or specimens and a shallower upper tray which distributes the water. Both are held in place by wooden frames. Both trays are perforated and the tapwater flow into the upper one is adjusted to keep the lower one nearly full. The correct number of holes needed in the bottom of each tray to maintain equilibrium of flow is ascertained easily by trial.
The burden of genetic disease on inpatient care in a children's hospital

The important role of genetics in pediatric illness has been increasingly recognized, but the true impact has not been well delineated. An important study of pediatric inpatient admissions to a children's hospital in 1978 found a genetic basis for disease in just less than half of admitted patients. We sought to update this study in light of current hospitalization practices and new knowledge about genetics. We systematically reviewed the records of 5,747 consecutive admissions (4,224 individuals), representing 98% of patients admitted in 1996 to Rainbow Babies and Children's Hospital (Cleveland, OH). Each patient was assigned to one of five groups on the basis of the presence or absence of an underlying chronic medical condition and whether that condition had a genetic basis or susceptibility. An underlying disorder with a significant genetic component was found in 71% of admitted children. The vast majority (96%) of underlying chronic disorders in children in this study were either clearly genetic or had a genetic susceptibility. Total charges for 1996 were >$62 million, of which $50 million (81%) was accounted for by disorders with a genetic determinant. The 34% of admissions with clearly genetic underlying disorders accounted for 50% (>$31 million) of the total hospital charges. The mean length of stay was 40% longer for individuals with an underlying disease with a genetic basis than for those with no underlying disease. Charges and length of stay were similar for children with underlying chronic disorders, regardless of the cause. This study begins to quantify the enormous impact of genetic disease on inpatient pediatrics and the health care system. Additional study and frank public discourse are needed to understand the implications on the future health care workforce and on the utilization of health care resources.
General Griffiths' Inequalities on Correlations in Ising Ferromagnets

Let N = (1, 2, ⋯, n). For each subset A of N, let JA ≥ 0. For eachi∈N, let σi ± 1. For each subset A of N, define σA=∏i∈A σi. Let the Hamiltonian be − ΣACN JA σA. Then for each A, B⊂N, 〈σA〉≥0 and 〈σAσB〉−〈σA〉〈σB〉≥0. This weakens the hypothesis and widens the conclusion of a result due to Griffiths.