cssi/SciSciGPT-SciSciNet huggingface dataset:
Surface energy balance and 24-h evapotranspiration on an agricultural landscape with SRF willow in central New York

Surface energy balance and 24-h evapotranspiration were mapped on an agricultural landscape with SRF willows using a remote sensing-based model to provide information on the water use by different land cover types. The results demonstrate the influence of land cover and vegetation type on surface energy balance and water losses. The evapotranspiration from forests was much higher than for other land cover types, corresponding to the significant cooling of the land surface. The SRF willow plantings were on average 3.6 °C cooler than the surrounding grassy areas; the 24-hour evapotranspiration from willows was 1.6 times higher than the evapotranspiration from surrounding grass, but lower than evapotranspiration from the forests. The possibility of changes in evapotranspiration patterns, local climate, and regional water balance with increasing acreage in SRF crop are discussed.
Learning, prediction and causal Bayes nets

Recent research in cognitive and developmental psychology on acquiring and using causal knowledge uses the causal Bayes net formalism, which simultaneously represents hypotheses about causal relations, probability relations, and effects of interventions. The formalism provides new normative standards for reinterpreting experiments on human judgment, offers a precise interpretation of mechanisms, and allows generalizations of existing theories of causal learning. Combined with hypotheses about learning algorithms, the formalism makes predictions about inferences in many experimental designs beyond the classical, Pavlovian cue-->effect design.
Inducible Knockout of the Interleukin-2 Receptor α Chain: Expression of the High-Affinity IL-2 Receptor Is Not Required for thein VitroGrowth of HTLV-I-Transformed Cell Lines

Adult T cell leukemia (ATL) is an aggressive malignancy that is associated with HTLV-I infection and characterized by constitutive expression of the high-affinity interleukin-2 receptor. The alpha subunit of the high-affinity receptor (IL-2Ralpha), which is normally present only on activated T cells, is specifically upregulated by HTLV-I and constitutively expressed on fresh leukemic cells from ATL patients as well as cell lines transformed by HTLV-I in vitro. Here we directly address the functional significance of IL-2Ralpha expression in HTLV-I transformed cell lines by using an endoplasmic reticulum-targeted single-chain antibody to inhibit the cell surface expression of IL-2Ralpha. Using constitutive and tetracycline-repressible systems to express the ER-targeted antibody against IL-2Ralpha, we have reduced cell surface expression of IL-2Ralpha by more that 2 logs of mean fluorescence intensity to virtually undetectable levels in the IL-2-independent HTLV-I-transformed cell lines C8166-45 and HUT102. No toxicity was associated with the intracellular retention of IL-2Ralpha, and the growth rate of the IL-2Ralpha-negative cells was in each case comparable to that of the parental cell line. We conclude that cell surface expression of IL-2Ralpha is dispensable for the in vitro growth of these HTLV-I-transformed cells.
Iron speciation and hydrogen peroxide concentrations in New Zealand rainwater

Abstract Rain was collected on the southern portion of the South Island of New Zealand during the summer of 1999 (January–March) during which time significant losses of ozone and increased UV were reported in the stratosphere over New Zealand. Iron and hydrogen peroxide concentrations were measured in rainwater because these analytes are directly influenced by photochemical processes and therefore are particularly susceptible to increasing UV levels. The absolute concentration of dissolved Fe(II) in New Zealand samples was very similar to summertime rain received in Wilmington, NC however the relative contribution of Fe(II) to total Fe was approximately twice as great in New Zealand samples. The larger percentage of reduced iron may reflect higher UV levels in New Zealand since Fe(II) is generated via photochemical reduction of particulate or dissolved Fe(III). No dissolved Fe(III) was detected in New Zealand rainwater, in contrast to the Wilmington site, where summertime Fe(III) concentrations are approximately equal to Fe(II) concentrations. Summertime hydrogen peroxide concentrations and diel variability in New Zealand were similar to other coastal and marine values in both the northern and southern hemispheres suggesting the increasing UV in New Zealand is not significantly increasing hydrogen peroxide concentrations at this location. Any excess photochemically produced hydrogen peroxide in New Zealand may be consumed through oxidation of Fe(II) which is rapidly reformed from photochemical reduction of Fe(III) by the higher UV levels in New Zealand.
Bomb-produced tritium in the Antarctic Ocean

Tritium measurements for twenty-four samples from the Antarctic Ocean are given. Results are discussed with respect to water mass types and previous measurements. High tritium concentrations are found in certain deep water masses, indicating an input of surface water into these masses in the last ten to twenty years.
Effect of unprocessed wheat bran oncalciuria and oxaluria in patients with urolithiasis

Abstract Seventeen hypercalciuria patients (8 control, 9 treatment) with a history of urolithiasis were randomly selected to receive low-calcium, low-oxalate diets with or without the addition of 30 g of dietary fiber as unprocessed wheat bran. Diet alone resulted in a 5.6 percent decrease in calciuria compared with a 23.5 percent decrease with the addition of the fiber. The addition of hydrochlorothiazide and potassium citrate further reduced calciuria by 40.4 percent and 34.5 percent respectively. Oxaluria was decreased 21.4 percent by diet alone compared with 3.9 percent in the diet and fiber treatment group. Patient compliance to diets was good, and no complications resulted from fiber intake.
What Vascular Ultrasound Testing Has Revealed about Pediatric Atherogenesis, and a Potential Clinical Role for Ultrasound in Pediatric Risk Assessment

Coronary vascular disease is one facet of a generalized disturbance of vascular function present throughout the vascular tree. Dysfunction of the endothelium leads to thickening of the intima and media of the vessel wall of large and medium-sized muscular arteries and large elastic arteries, such as the aorta, carotid, and iliac arteries. Flow-mediated dilatation of the brachial artery is one of several tests used to assess dysfunction of the endothelium using high resolution ultrasound. Endothelial dysfunction has been demonstrated in children with heterozygous familial hypercholesterolemia, type 1 diabetes, morbid obesity, and homozygous homocystinuria and in the offspring of a parent with early coronary disease. High resolution ultrasound has also confirmed postmortem findings that atherogenesis has its beginnings in childhood and adolescence, with the demonstration of increased carotid artery intima-medial thickening in children with familial hypercholesterolemia, familial combined hyperlipidemia, and type 1 diabetes and in the offspring of a parent with early coronary disease. In combination with family history and traditional risk factors, ultrasound evaluation of brachial artery flow-mediated vasodilation and carotid artery intima-medial thickening could be used in a clinical setting to assess coronary risk in high risk pediatric patients.
Transient anabolic effects accompany epidermal growth factor receptor signal activation in articular cartilage in vivo

Signals from the epidermal growth factor receptor (EGFR) have typically been considered to provide catabolic activities in articular cartilage, and accordingly have been suggested to have a causal role in osteoarthritis progression. The aim of this study was to determine in vivo roles for endogenous EGFR signal activation in articular cartilage. Transgenic mice with conditional, limb-targeted deletion of the endogenous intracellular EGFR inhibitor Mig-6 were generated using CreLoxP (Mig-6-flox; Prx1Cre) recombination. Histology, histochemical staining and immunohistochemistry were used to confirm activation of EGFR signaling in the articular cartilage and joints, and to analyze phenotypic consequences of Mig-6 loss on articular cartilage morphology, proliferation, expression of progenitor cell markers, presence of chondrocyte hypertrophy and degradation of articular cartilage matrix. The articular cartilage of Mig-6-conditional knockout (Mig-6-cko) mice was dramatically and significantly thicker than normal articular cartilage at 6 and 12 weeks of age. Mig-6-cko articular cartilage contained a population of chondrocytes in which EGFR signaling was activated, and which were three to four times more proliferative than normal Mig-6-flox articular chondrocytes. These cells expressed high levels of the master chondrogenic regulatory factor Sox9, as well as high levels of putative progenitor cell markers including superficial zone protein (SZP), growth and differentiation factor-5 (GDF-5) and Notch1. Expression levels were also high for activated β-catenin and the transforming growth factor beta (TGF-β) mediators phospho-Smad2/3 (pSmad2/3). Anabolic effects of EGFR activation in articular cartilage were followed by catabolic events, including matrix degradation, as determined by accumulation of aggrecan cleavage fragments, and onset of hypertrophy as determined by type × collagen expression. By 16 weeks of age, the articular cartilage of Mig-6-cko knees was no longer thickened and was degenerating. These results demonstrate unexpected anabolic effects of EGFR signal activation in articular cartilage, and suggest the hypothesis that these effects may promote the expansion and/or activity of an endogenous EGFR-responsive cell population within the articular cartilage.
Multiscale bilateral filtering for improving image quality in digital breast tomosynthesis.

Purpose: Detection of subtle microcalcifications in digital breast tomosynthesis (DBT) is a challenging task because of the large, noisy DBT volume. It is important to enhance the contrast-to-noise ratio (CNR) of microcalcifications in DBT reconstruction. Most regularization methods depend on local gradient and may treat the ill-defined margins or subtle spiculations of masses and subtle microcalcifications as noise because of their small gradient. The authors developed a new multiscale bilateral filtering (MSBF) regularization method for the simultaneous algebraic reconstruction technique (SART) to improve the CNR of microcalcifications without compromising the quality of masses. Methods: The MSBF exploits a multiscale structure of DBT images to suppress noise and selectively enhance high frequency structures. At the end of each SART iteration, every DBT slice is decomposed into several frequency bands via Laplacian pyramid decomposition. No regularization is applied to the low frequency bands so that subtle edges of masses and structured background are preserved. Bilateral filtering is applied to the high frequency bands to enhance microcalcifications while suppressing noise. The regularized DBT images are used for updating in the next SART iteration. The new MSBF method was compared with the nonconvex total p-variation (TpV) method for noise regularization with SART. A GE GEN2 prototype DBT system was used for acquisition of projections at 21 angles in 3° increments over a ±30° range. The reconstruction image quality with no regularization (NR) and that with the two regularization methods were compared using the DBT scans of a heterogeneous breast phantom and several human subjects with masses and microcalcifications. The CNR and the full width at half maximum (FWHM) of the line profiles of microcalcifications and across the spiculations within their in-focus DBT slices were used as image quality measures. Results: The MSBF method reduced contouring artifacts and enhanced the CNR of microcalcifications compared to the TpV method, thus preserving the image quality of the structured background. The MSBF method achieved the highest CNR of microcalcifications among the three methods. The FWHM of the microcalcifications and mass spiculations resulting from the MSBF method was comparable to that without regularization, and superior to that of the TpV method. Conclusions: The SART regularized by the multiscale bilateral filtering method enhanced the CNR of microcalcifications and preserved the sharpness of microcalcifications and spiculated masses. The MSBF method provided better image quality of the structured background and was superior to TpV and NR for enhancing microcalcifications while preserving the appearance of mass margins.
Slit grafting: the use of serrated island grafts in male and female-pattern alopecia.

Successful aesthetic treatment of earlier thinning of scalp hair in men and women has necessitated the development of alternatives to traditional punch grafting. The hair-bearing “serrated island” remaining between two parallel rows of round donor sites can be removed and dissected to yield a large number of “serrated island grafts” and single-hair grafts. These miniature grafts, along with half grafts from bisected plugs, can be placed, in one or several sessions, into dozens to hundreds of small “slits” between the remaining hairs in patients with early or advanced thinning on the crown, vertex, or anterior scalp — hence the name “slit grafting.” The versatility of this technique is demonstrated by its value for patients with dark hair and light skin, in repair work, in patients necessitating extensive vertex coverage, or in combination with alopecia reduction and traditional punch grafting. The finely tufted progressive growth from hundreds of these carefully placed slit grafts can thus counteract further hair loss in a variety of different situations.
The Economic Impacts of Disease Suppressive Rotations in Maine Potato Cropping Systems

Soil borne diseases can greatly reduce marketable yields and are a major concern to Maine potato (Solanum tuberosum L.) producers. Control of these organisms is difficult as they can persist in the soil for several years and symptoms typically worsen each year. Depending on the specific pathogen, different mechanisms for moderating soil borne disease can be employed, such as breaking the hostpathogen cycle, stimulating beneficial microbial activity, and direct inhibition of the pathogens. Current research at the USDA-ARS New England Plant, Soil, and Water Laboratory is investigating the effect of rotation crops and rotation sequences on the incidence and severity of soil-borne diseases in the following potato crop. These results (incidence, severity, and potato yield) are incorporated into an economic simulation model to determine the impact of rotations on profitability, level of income risk, and probability of economic loss for different rotation sequences and rotation lengths. The results show that several rotation sequences can increase profitability and reduce economic risk when compared to continuous potato or to the standard barley-potato rotations.
Characterization of Near-Infrared Atomic Emission from a Radio-Frequency Plasma for Selective Detection in Capillary Gas Chromatography:

A radio-frequency plasma source has been characterized for use as an element-selective detector with capillary gas chromatography using atomic emission in the near-infrared region of the spectrum. With selected compounds introduced at rates similar to those encountered during a chromatographic run, the performance of the plasma has been characterized as a function of several interdependent variables, including plasma makeup gas flow, electrode spacing, and applied power. These settings have been optimized, and detection limits for several nonmetallic elements determined. Detection limits range from 0.2 to 50 pg/s.
Amniotic Prolactin Control Over Amniotic and Fetal Extracellular Fluid Water and Electrolytes in the Rhesus Monkey

Administration of 1–10 mg ovine pituitary prolactin (oPRL) into the amniotic fluid of 10 rhesus monkeys in the last third of gestation consistently caused a decrease in amniotic fluid volume not seen when saline, vasopressin, or bovine serum albumin were injected into 9 other monkeys. The effects lasted for 24 h. Intraamniotic injection of 10 mg oPRL prevented or reversed a doubling of water and electrolyte content of the fetal extracellular fluid (ECF) volume in the face of hypertonic amniotic fluid. Efflux of these substances from the fetal ECF in the face of hypotonic amniotic fluid was similarly prevented or reversed by intraamniotic prolactin injection. Ovine PRL had no effect on fetal ECF water and electrolytes in the face of isotonic amniotic fluid. Possible sites of these oPRL effects were amnion, placenta, fetal lung and/or fetal gastrointestinal tract. (Endocrinology 100: 564, 1977)
Comparison of apnea identified by respiratory inductance plethysmography with that detected by end-tidal CO2 or thermistor

As part of the Collaborative Home Infant Monitoring Evaluation (CHIME) we compared apnea identified by a customized home monitor using respiratory inductance plethysmography (RIP) with simultaneously recorded polysomnography-acquired nasal end-tidal CO2 (Pet CO2 ) and nasal/oral thermistor in 422 infants during overnight laboratory recordings to determine concordance between techniques, sources of disagreement, and capacity of RIP to detect obstructed breaths within an apnea. Among 233 episodes of apnea identified by at least one method as ⩾ 16 s, 120 were observed by the CHIME monitor, 219 by Pet CO2 , and 163 by thermistor. The positive predictive value of the CHIME-identified apnea was 89.2% (95% CI 83, 95) and 73% (95% CI 65, 81) for Pet CO2 and thermistor, respectively. However, the sensitivity of the CHIME monitor in identifying events detected by the other methods was only ∼ 50%. Among 87 apnea events identified by all three techniques, no two methods showed high agreement in measurement of apnea d...
Vision therapy: are you kidding me? Problems with current studies.

Orthoptic exercises are routinely prescribed to treat symptoms of convergence insufficiency (CI). The type and duration of treatment tends to vary among providers as until recently, few strong rand...
Chloride flux out of Yellowstone National Park

Abstract Monitoring of the chloride concentration, electrical conductivity, and discharge was carried out for the four major rivers of Yellowstone National Park from September 1982 to January 1984. Chloride flux out of the Park was determined from the measured values of chloride concentration and discharge. The annual chloride flux from the Park was 5.86 × 1010 g. Of this amount 45% was from the Madison River drainage basin, 32% from the Yellowstone River basin, 12% from the Snake River basin, and 11% from the Falls River basin. Of the annual chloride flux from the Yellowstone River drainage basin 36% was attributed to the Yellowstone Lake drainage basin. The geothermal contribution to the chloride flux was determined by subtracting the chloride contribution from rock weathering and atmospheric precipitation and is 94% of the total chloride flux. Calculations of the geothermal chloride flux for each river are given and the implications of an additional chloride flux out of the western Park boundary discussed. An anomalous increase in chloride flux out of the Park was observed for several weeks prior to the Mt. Borah earthquake in Central Idaho on October 28, 1983, reaching a peak value shortly thereafter. It is suggested that the rise in flux was a precursor of the earthquake. The information in this paper provides baseline data against which future changes in the hydrothermal systems can be measured. It also provides measurements related to the thermal contributions from the different drainage basins of the Park.
Neurosonographic imaging of small-for-gestational-age neonates exposed and not exposed to cocaine and cytomegalovirus.

We sought to prospectively identify the role of neurosonography in the evaluation of a consecutive group of small-for-gestational-age (SGA) neonates, and also to identify the association of neurosonographic findings with cocaine exposure and cytomegalovirus (CMV) infection. Neurosonographic imaging was performed in 180 SGA neonates within 72 hours of birth. Urine samples were screened for CMV and cocaine metabolites (CM) in all cases. Sixty-five neonates (37.5%) had an abnormal neurosonographic appearance. Nine neonates were positive for CMV and 31 neonates were positive for CM. Focal echolucencies (27), ventricular dilation (27), and subependymal hemorrhages (12) were the most common neurosonographic abnormalities. The first two were more common with CM (p < .05). An abnormal neurosonographic pattern was seen more often in SGA neonates with CM (54.8%, 17 of 31; p < .05) and CMV (67%, 6 of 9; p < .01) as compared with the rest (32.6%, 44 of 135; p < .01). Among those without CM or CMV, prematurity was associated with an increased risk for abnormality (p < .001 between groups), specifically subependymal hemorrhage, ventricular dilation, and porencephalic cysts. Five CMV-positive neonates showed periventricular, echogenic foci mainly in the area of the frontal horn. Two new findings with SGA were caudate nucleus echogenicity and a “moth-eaten” appearance of the thalamus, each found in three infants. Neurosonographic imaging is useful in the evaluation of SGA neonates. Focal echolucencies and caudate echogenicity suggest maternal cocaine use, and periventricular echogenic foci strongly suggest fetal CMV infection. © 1994 John Wiley & Sons, Inc.
Ectopic pregnancy concurrent with induced abortion: Incidence and mortality

From 1972 through 1985, 24 women who underwent an induced abortion died as a result of a concurrent ectopic pregnancy. We analyzed data from the Joint Program for the Study of Abortion, National Hospital Discharge Survey, and the Centers for Disease Control Ectopic Pregnancy and Abortion Surveillance Systems to determine the incidence and mortality of ectopic pregnancy concurrent with induced abortion. During the period 1971 through 1985, the incidence of ectopic pregnancy concurrent with induced abortions was 1.35/1000 induced abortions, compared with 13.6/1000 pregnancies not terminated by induced or spontaneous abortion. The rate was higher among women who obtained abortions at earlier gestational age and among older women. The death-to-case rate for ectopic pregnancies concurrent with induced abortion was 1.3 times higher than that for women not undergoing abortion. Most of the deaths of women with ectopic pregnancy who underwent induced abortion were attributable to the failure to diagnose the ectopic pregnancy before the woman left the facility where the abortion was performed. Such deaths could be prevented by the provider of the abortion assuring that the tissue is examined for products of conception at the time of the abortion.
Side-by-Side Comparison of Gene-Based Smallpox Vaccine with MVA in Nonhuman Primates

Orthopoxviruses remain a threat as biological weapons and zoonoses. The licensed live-virus vaccine is associated with serious health risks, making its general usage unacceptable. Attenuated vaccines are being developed as alternatives, the most advanced of which is modified-vaccinia virus Ankara (MVA). We previously developed a gene-based vaccine, termed 4pox, which targets four orthopoxvirus antigens, A33, B5, A27 and L1. This vaccine protects mice and non-human primates from lethal orthopoxvirus disease. Here, we investigated the capacity of the molecular adjuvants GM-CSF and Escherichia coli heat-labile enterotoxin (LT) to enhance the efficacy of the 4pox gene-based vaccine. Both adjuvants significantly increased protective antibody responses in mice. We directly compared the 4pox plus LT vaccine against MVA in a monkeypox virus (MPXV) nonhuman primate (NHP) challenge model. NHPs were vaccinated twice with MVA by intramuscular injection or the 4pox/LT vaccine delivered using a disposable gene gun device. As a positive control, one NHP was vaccinated with ACAM2000. NHPs vaccinated with each vaccine developed anti-orthopoxvirus antibody responses, including those against the 4pox antigens. After MPXV intravenous challenge, all control NHPs developed severe disease, while the ACAM2000 vaccinated animal was well protected. All NHPs vaccinated with MVA were protected from lethality, but three of five developed severe disease and all animals shed virus. All five NHPs vaccinated with 4pox/LT survived and only one developed severe disease. None of the 4pox/LT-vaccinated animals shed virus. Our findings show, for the first time, that a subunit orthopoxvirus vaccine delivered by the same schedule can provide a degree of protection at least as high as that of MVA.
Fractal analysis of virtual endoscopy reconstructions

Virtual endoscopy reconstructions of the body noninvasively provide morphologic information of gross structural abnormalities such as stenoses in airways or blood vessels and polyps in the colonic wall. Surface irregularity or roughness is another indication of abnormality potentially detectible on virtual endoscopy. In this paper, we show how fractal dimension can be used to quantify surface roughness and how these methods may be applied to virtual angioscopy to distinguish the thoracic aorta in a normal volunteer from that of a patient predisposed to atherosclerosis. Finally, we discuss some problems we encountered applying fractal analysis to small, noisy datasets.
Recent temperature extremes at high northern latitudes unprecedented in the past 600 years

By use of a hierarchical Bayesian analysis of instrumental and proxy temperature records, early twenty-first-century summers at high northern latitudes are shown to have been warmer than any since 1400. Exceptionally high temperatures have been observed in Greenland, Russia and other high northern latitudes in the past decade. But until now it has not been possible to establish the degree to which periods of extreme warmth are anomalous relative to historical variability. A more rigorous statistical approach is needed for that. Now, using a hierarchical Bayesian approach and several climate proxies, Martin Tingley and Peter Huybers show that early-twenty-first-century summers in the high north have been warmer than any since 1400. These recent warm temperature extremes are unprecedented relative to temperature trends in the past 600 years. Recently observed extreme temperatures at high northern latitudes1,2,3,4,5,6,7 are rare by definition, making the longer time span afforded by climate proxies important for assessing how the frequency of such extremes may be changing. Previous reconstructions of past temperature variability have demonstrated that recent warmth is anomalous relative to preceding centuries2,8,9 or millennia10, but extreme events can be more thoroughly evaluated using a spatially resolved approach that provides an ensemble of possible temperature histories11,12. Here, using a hierarchical Bayesian analysis13,14 of instrumental, tree-ring, ice-core and lake-sediment records, we show that the magnitude and frequency of recent warm temperature extremes at high northern latitudes are unprecedented in the past 600 years. The summers of 2005, 2007, 2010 and 2011 were warmer than those of all prior years back to 1400 (probability P > 0.95), in terms of the spatial average. The summer of 2010 was the warmest in the previous 600 years in western Russia (P > 0.99) and probably the warmest in western Greenland and the Canadian Arctic as well (P > 0.90). These and other recent extremes greatly exceed those expected from a stationary climate, but can be understood as resulting from constant space–time variability about an increased mean temperature.
Lighting of End of Lay Broiler Breeders: Fluorescent Versus Incandescent

Abstract An 18-week experiment was conducted to investigate the effects of changing from incandescent to fluorescent lighting on egg production, egg weight, fertility, and hatchability of end of lay broiler breeders housed in an open-sided house. Forty-eight-week-old Cobb feather-sexed broiler breeders were housed, 30 females and 3 males per pen, in a total of 28 pens. Incandescent lights had been used previously, so pens were randomly assigned to either fluorescent or incandescent lights giving 20 lx of light at bird level. Lights used were 60 W incandescent and 22 W fluorescent cool-white circular. Body weight and egg production were measured weekly, and fertility, hatchability, and egg weight were determined monthly from 48 to 65 weeks of age. No significant treatment effects were observed on body weight, fertility, hatchability, or egg weight. A significant reduction in egg production was observed with fluorescent lighting from Weeks 58 to 65. The reduced egg production indicated it was detrimental to change from incandescent to cool-white fluorescent lighting.
Characterizing the Effects of Highly Bent DNA on Transcription

It is well-established that many transcriptional repressors such as the loop-forming lactose or galactose repressors tightly bend DNA. However, it remains unclear whether this bending directly affects the activity of RNA polymerase. Characterizing the effects of repressor-bound, highly stressed DNA on transcription is complicated by the inability to decouple the mechanical state of the DNA template from the kinetics of repressor binding, which itself will be influenced by the energy required for the repressor to strain the DNA. To our knowledge there currently exists no established assay capable of quantifying transcription by RNA polymerase from highly bent DNA templates which mimic the bending induced by loop-forming repressors in the absence of other DNA-binding proteins. We have developed a fluorescence-based in vitro assay capable of addressing this experimental limitation by exploiting the ability of DNA minicircles to impose both varying degrees of bending and twist. We are now applying this assay to characterize the rates of transcription by T7 RNA polymerase from highly stressed templates. We hypothesize that the activity of T7 RNA polymerase will be reduced on templates that are bent with degrees of curvature comparable to the loops generated by loop-forming repressor proteins. Contrary to our initial hypothesis, preliminary data suggests that at least one such highly bent DNA template is in fact readily transcribed by T7 RNA polymerase. However, the degree to which the minicircle templates are twisted beyond the torsionally relaxed helical structure may play a greater-than-expected role on the activity of RNA polymerase, and must therefore be addressed. Conclusions resulting from these measurements will shed light on the mechanism by which loop-forming repressors affect transcription, and could reveal a more significant role for the mechanical state of the DNA than was previously thought.
Perioperative complications in obese women vs normal-weight women who undergo vaginal surgery

Objective The purpose of this study was to compare the incidence of perioperative complications in obese and normal-weight patients who undergo vaginal urogynecologic surgery. Study Design A retrospective cohort analysis was conducted for obese patients (body mass index, ≥30 kg/m 2 ) who underwent vaginal surgery and who were matched with patients with normal body mass indices (>18.5 kg/m 2 but 2 ) by surgical procedures. Demographic information, comorbidities, and perioperative (≤6 weeks) complications were documented. Logistic regression analysis was used to compare the incidence of perioperative complications and to adjust for baseline differences. Results Seven hundred forty-two patients underwent vaginal surgery during the study period; 235 women were considered to have obese body mass indices. We matched 194 of these patients with normal-weight control subjects. There was no statistical difference in the proportion of subjects who had at least 1 perioperative complication (20% [obese] vs 15% [nonobese]). However, obese subjects were more likely to have an operative site infection (adjusted odds ratio, 5.5; [95% CI, 1.7-24.7]; P = .01). Conclusion The overall perioperative complication rate in obese and nonobese women is low, with obesity as an independent risk factor for the development of operative site infections.
Genotype-Based Bayesian Analysis of Gene-Environment Interactions with Multiple Genetic Markers and Misclassification in Environmental Factors

A key component to understanding etiology of complex diseases, such as cancer, diabetes, alcohol dependence, is to investigate gene-environment interactions. This work is motivated by the following two concerns in the analysis of gene-environment interactions. First, multiple genetic markers in moderate linkage disequilibrium may be involved in susceptibility to a complex disease. Second, environmental factors may be subject to misclassification. We develop a genotype based Bayesian pseudolikelihood approach that accommodates linkage disequilibrium in genetic markers and misclassification in environmental factors. Since our approach is genotype based, it allows the observed genetic information to enter the model directly thus eliminating the need to infer haplotype phase and simplifying computations. Bayesian approach allows shrinking parameter estimates towards prior distribution to improve estimation and inference when environmental factors are subject to misclassification. Simulation experiments demonstrated that our method produced parameter estimates that are nearly unbiased even for small sample sizes. An application of our method is illustrated using a case-control study of interaction between early onset of drinking and genes involved in dopamine pathway.
Analysis of proton exchange membrane fuel cell polarization losses at elevated temperature 120 °C and reduced relative humidity

Abstract Polarization losses of proton exchange membrane (PEM) fuel cells at 120 °C and reduced relative humidity (RH) were analyzed. Reduced RH affects membrane and electrode ionic resistance, catalytic activity and oxygen transport. For a cell made of Nafion ® 112 membrane and electrodes that have 35 wt.% Nafion ® and 0.3 mg/cm 2 platinum supported on carbon, membrane resistance at 20%RH was 0.407 Ω cm 2 and electrode resistance 0.203 Ω cm 2 , significantly higher than 0.092 and 0.041 Ω cm 2 at 100%RH, respectively. In the kinetically controlled region, 20%RH resulted in 96 mV more cathode activation loss than 100%RH. Compared to 100%, 20%RH also produced significant oxygen transport loss across the ionomer film in the electrode, 105 mV at 600 mA/cm 2 . The significant increase in polarization losses at elevated temperature and reduced RH indicates the extreme importance of designing electrodes for high temperature PEM fuel cells since membrane development has always taken most emphasis.
Computability and complexity of ray tracing

The ray-tracing problem is, given an optical system and the position and direction of an initial light ray, to decide if the light ray reaches some given final position. For many years ray tracing has been used for designing and analyzing optical systems. Ray tracing is now used extensively in computer graphics to render scenes with complex curved objects under global illumination. We show that ray-tracing problems in some three-dimensional simple optical systems (purely geometrical optics) are undecidable. These systems may consist of either reflective objects that are represented by rational quadratic equations, or refractive objects that are represented by rational linear equations. Some problems in more restricted models are shown to be PSPACE-hard or sometimes in PSPACE.
A note on polymeric inner-layered single fiber interfacial strength determination

The role of inner-layer (interphase) in fiber-reinforced composites is often assessed by measuring the interfacial shear strength of single fibers embedded in matrix blocks. Interfacial shear strength is calculated using simple shear lag analyses (Kelly and Tyson model). This paper shows that application of Kelly and Tyson's equation may result in gross overprediction of interfacial shear strengths if the thickness of the inner-layer is not negligible in comparison with the fiber diameter.
The Meridional Variation of the Eddy Heat Fluxes by Baroclinic Waves and Their Parameterization

The meridional and vertical eddy fluxes of sensible heat produced by small-amplitude growing baroclinic waves are calculated using solutions to the two-level model with horizontal shear in the mean flow. The results show that the fluxes are primarily dependent on the local baroclinicity, i.e., the local value of the isentropic slopes in the mean state. Where the slope exceeds the critical value, the transports are poleward and upward; where the slope is less than the critical value, the transports are equatorward and downward. These results are used to improve an earlier parameterization of the tropospheric eddy fluxes of sensible heat based on Eady's model. Comparisons with observations show that the improved parameterization reproduces the observed magnitude and sign of the eddy fluxes and their vertical variations and seasonal changes, but the maximum in the poleward flux is too near the equator.
Dishevelled binds the Discs large 'Hook' domain to activate GukHolder-dependent spindle positioning in Drosophila.

Communication between cortical cell polarity cues and the mitotic spindle ensures proper orientation of cell divisions within complex tissues. Defects in mitotic spindle positioning have been linked to various developmental disorders and have recently emerged as a potential contributor to tumorigenesis. Despite the importance of this process to human health, the molecular mechanisms that regulate spindle orientation are not fully understood. Moreover, it remains unclear how diverse cortical polarity complexes might cooperate to influence spindle positioning. We and others have demonstrated spindle orientation roles for Dishevelled (Dsh), a key regulator of planar cell polarity, and Discs large (Dlg), a conserved apico-basal cell polarity regulator, effects which were previously thought to operate within distinct molecular pathways. Here we identify a novel direct interaction between the Dsh-PDZ domain and the alternatively spliced “I3-insert” of the Dlg-Hook domain, thus establishing a potential convergent Dsh/Dlg pathway. Furthermore, we identify a Dlg sequence motif necessary for the Dsh interaction that shares homology to the site of Dsh binding in the Frizzled receptor. Expression of Dsh enhanced Dlg-mediated spindle positioning similar to deletion of the Hook domain. This Dsh-mediated activation was dependent on the Dlg-binding partner, GukHolder (GukH). These results suggest that Dsh binding may regulate core interdomain conformational dynamics previously described for Dlg. Together, our results identify Dlg as an effector of Dsh signaling and demonstrate a Dsh-mediated mechanism for the activation of Dlg/GukH-dependent spindle positioning. Cooperation between these two evolutionarily-conserved cell polarity pathways could have important implications to both the development and maintenance of tissue homeostasis in animals.
Use of the implicit association test for the measurement of tinnitus-related distress.

Purpose A priming stimulus activates and increases an association with the target stimulus. The goal of this research was to investigate whether current tinnitus measures are susceptible to increased error due to priming and, if so, to examine the feasibility of using the Implicit Association Test (IAT; Greenwald, McGhee, & Schwartz, 1998) for an alternative measurement of tinnitus-related distress. Method Participants completed 2 tinnitus-related questionnaires and the IAT online. Results Although participants with tinnitus did not view sound-related words as significantly more negative and IAT scores did not predict scores on the Tinnitus Handicap Inventory (Newman, Jacobson, & Spitzer, 1996), priming did affect negative implicit attitudes toward sound-related words. Conclusions On the basis of these results, it is suggested that current tinnitus measures may be susceptible to priming error and that future studies should continue to pursue how the IAT can be utilized in the measure of tinnitus-related d...
Two-dimensional convection and radiation with scattering from a Poiseuille flow

Two-dimensional combined convection and radiation heat transfer from a gray scattering fluid in a reflecting channel is considered. The model, represented by a set of simultaneous nonlinear integro-partial differential equations, is solved numerically. The effects of aspect ratio, conduction-radiation parameter, scattering albedo, and wall emissivity, are systematically investigated. It is found that these parameters have a significant influence on the temperature field and alter the radiative and convective fluxes at the hot and cold walls. In particular, when radiation effects are considerable, the heat-transfer characteristics of the fluid at the hot and cold walls are very different.
Subcutaneous administration of muscarinic antagonists and triple-immunostaining of the levator auris longus muscle in mice.

Hind limb muscles of rodents, such as gastrocnemius and tibialis anterior, are frequently used for in vivo pharmacological studies of the signals essential for the formation and maintenance of mammalian NMJs. However, drug penetration into these muscles after subcutaneous or intramuscular administration is often incomplete or uneven and many NMJs can remain unaffected. Although systemic administration with devices such as mini-pumps can improve the spatiotemporal effects, the invasive nature of this approach can cause confounding inflammatory responses and/or direct muscle damage. Moreover, complete analysis of the NMJs in a hind limb muscle is challenging because it requires time-consuming serial sectioning and extensive immunostaining. The mouse LAL is a thin, flat sheet of muscle located superficially on the dorsum of the neck. It is a fast-twitch muscle that functions to move the pinna. It contains rostral and caudal portions that originate from the midline of the cranium and extend laterally to the cartilaginous portion of each pinna. The muscle is supplied by a branch of the facial nerve that projects caudally as it exits the stylomastoid foramen. We and others have found LAL to be a convenient preparation that offers advantages for the investigation of both short and long-term in vivo effects of drugs on NMJs and muscles. First, its superficial location facilitates multiple local applications of drugs under light anesthesia. Second, its thinness (2-3 layers of muscle fibers) permits visualization and analysis of almost all the NMJs within the muscle. Third, the ease of dissecting it with its nerve intact together with the pattern of its innervation permits supplementary electrophysiological analysis in vitro9,5. Last, and perhaps most importantly, a small applied volume (˜50μl) easily covers the entire muscle surface, provides a uniform and prolonged exposure of all its NMJs to the drug and eliminates the need for a systemic approach1,8.
Can Government Support the Press? Historicizing and Internationalizing a Policy Approach to the Journalism Crisis

Although consensus has been reached that journalism is in crisis, few agree on the nature of its decline or the means of resolving it. After providing an overview of this ongoing debate, the author contextualizes the journalism crisis within several historical, political, intellectual, and structural processes that help highlight what is at stake and what can be done to support the journalism required for a democratic society in the United States. He concludes with an emphasis on normative considerations regarding the future of journalism and provides several policy proposals that aim to provide the resources necessary for a robust and independent press. These policies, he suggests, will help facilitate the transition from a failing commercial media system to a public-service model of the press.
Dendritic anion hosts: perchlorate uptake by G5-NH2 poly(propyleneimine) dendrimer in water and model electrolyte solutions.

Perchlorate (ClO_4^-) has emerged as a major groundwater and surface water contaminant in the United States. Ion exchange (IX) is the most widely used technology for treating water containing lower concentrations of perchlorate (<100 ppb). However, a major drawback of IX is the need for frequent regeneration or disposal of the perchlorate-laden resins. As a first step toward the development of high-capacity, selective and recyclable dendritic ligands for the recovery of perchlorate from aqueous solutions by dendrimer filtration, we tested the hypothesis that dendrimers with hydrophobic cavities and positively charged internal groups should selectively bind ClO_4^- over more hydrophilic anions such as Cl^-, NO_3^-, SO_4^(2-), and HCO_3^-. We measured the uptake of ClO_4^- by the fifth generation (G5-NH_2) poly(propyleneimine) (PPI) dendrimer with a diaminobutane core and terminal NH_2 groups in deonized water and model electrolyte solutions as a function of (i) anion−dendrimer loading, (ii) solution pH, (iii) background electrolyte concentration, and (iv) reaction time. The ClO_4^- binding capacity of this dendrimer is comparable to those of perchlorate-selective IX resins. However, its ClO_4^- binding kinetics is faster and reaches equilibrium in ∼1 h. Note also that only a high pH (∼9.0) aqueous solution is needed to release more than 90% of the bound ClO_4^- anions by deprotonation of the dendrimer tertiary amine groups. The overall results of this study suggest that dendritic macromolecules such as the G5-PPI NH_2 dendrimer provide ideal building blocks for the development of high-capacity, selective and recyclable ligands for the recovery of anions such as perchlorate from aqueous solutions by dendrimer enhanced filtration.
Sulfluramid resistance and vapor toxicity in field-collected German cockroaches (Dictyoptera: Blattellidae).

The toxicities of Raid Max Roach Bait (sulfluramid) and COMBAT Roach Control System (hydramethylnon) to susceptible and field-collected German cockroaches were examined. In all field-collected strains, a variable fraction of the population exhibited tolerance to Raid Max. In some strains, few males were killed in the first 5 d of exposure to Raid Max and some lived for up to 123 d when provided Raid Max only. Field-collected males that were given access to Raid Max for only 3 h following a 45·h starvation exhibited a 22-fold delay in mortality of 50% of the population compared with males that were continuously exposed to Raid Max. Males exposed to COMBAT for 3 h exhibited a similar pattern of mortality as males continuously exposed to this bait. Field-collected males provided COMBAT with or without rat chow exhibited identical patterns of mortality. However, males that were offered Raid Max along with rat chow exhibited significantly delayed mortality compared with males given Raid Max alone. A direct comparison of 1% hydramethylnon and sulfluramid, the active ingredients in COMBAT and Raid Max, respectively, in rat chow showed that physiological resistance to sulfluramid was involved; field-collected males consumed both baits equally on the first day, but whereas 100% of the males that were fed hydramethylnon-containing chow died within 5 d, only one of 25 males fed sulfluramid-baited rat chow died during this period, and males continued to consume large amounts of food. This suggested that, in the presence of alternate foods, the effective dose of sulfluramid was diminished, resulting in reduced mortality in males fed Raid Max. These results suggest that relatively high levels of resistance to sulfluramid are pervasive in field populations of the German cockroach. Experiments in which cockroaches were exposed to vapors of Raid Max or sulfluramid without direct contact showed that both acted as fumigants. The Raid Max bait remained lethal without direct contact for at least 170 d of continuous aeration in a fume hood. A headspace analysis revealed that sulfluramid was present in airborne collections of both technical sulfluramid and the Raid Max bait.