cssi/SciSciGPT-SciSciNet huggingface dataset:
Pathophysiology of Hemorrhagic Shock and Resuscitation

Shock may be defined broadly as a condition in which metabolic energy production is limited either by the supply or utilization of oxygen, manifest by derangement of the normal oxygen supply–demand balance, accumulation of the byproducts of anaerobic metabolism, and dysfunction of one or more organ system. In the case of massive hemorrhage, shock is due specifically to impaired oxygen delivery (VO2) secondary to both hypovolemia and anemia. Restoration of tissue perfusion, termed resuscitation, proceeds systematically, is based upon the underlying etiology of shock, terminates upon achievement of clearly defined endpoints, and requires frequent re-evaluation. Resuscitation has been refined substantially over the previous decade, with a resultant improvement in the outcomes of critically ill patients (Brun-Buisson et al. 2004; Martin et al. 2003). Major changes have included improved accuracy of the assessment of intravascular volume status, recognition of the detrimental effects of both allogeneic blood product transfusion and excessive volume expansion, and timely, goal-directed treatment of shock and its complications. This chapter will focus on the diagnosis of shock, differentiation into hemorrhagic shock, the benefits and limitations of various measurements used to determine the adequacy of resuscitation, general resuscitative strategies, and complications of resuscitation.
Quantifying the capacity value of natural gas efficiency in New England

Abstract Natural gas utilities in New England face increasing natural gas transmission system congestion and volatile spot gas prices. We observe that prevailing evaluation methods for natural gas efficiency programs value avoided firm pipeline capacity based on its total (‘gross') cost. We propose, consistent with deregulated electricity markets, to value avoided firm pipeline capacity at its ‘net’ cost. Specifically, we account for revenues associated with selling excess capacity during periods when the firm capacity holder does not fully utilize its rights. Our evaluation of commercial building efficiency programs suggests that prevailing evaluation methods could over-estimate the value of efficiency programs when those programs are intended to offset new capacity investments (i.e., the utility is in need of additional firm capacity). The situation is more complex for a utility with sufficient firm pipeline capacity to meet forecast load (i.e., consumption). In this case, the prevailing evaluation methods have the potential to under- or over-estimate the value of efficiency programs. The value of avoided infrastructure investments is likely to vary with the specific circumstances of the utility and the expected revenues associated with short-term sales enabled by available capacity. In the future, we recommend that economic regulators of natural gas distribution utilities, the state public utility commissions (PUCs), value avoided infrastructure investments at the ‘net' cost of the investment instead of the ‘gross' cost.
Jobs and Community in Humboldt County, California

During the last three decades, ecological restoration has grown from a locally rooted, community-based movement into a widespread practice, diverse in meaning, application, and scale. During this period, restoration has become institutionalized as an important activity within a multitude of national, state, and local government agencies and programs. Restoration now represents a legitimate form of scientific inquiry and scholarship, supported by university research and teaching programs, professional associations, and journals. Perhaps most important, private and public sector funding for landscape-scale and other restoration efforts has steadily increased. Yet the phenomenal growth of the restoration movement, along with the increasing legitimacy accorded particular forms of restoration, has raised the stakes of debates about restoration’s purpose and rationale. Growing demand and esteem for restoration have illuminated its dynamic meaning—it ranges from a site for the productive engagement of communities and environments to a science-based practice that allows for the efficient and large-scale restitution of damaged ecosystems.
Greenland Ice Mapping Project: ice flow velocity variation at sub-monthly to decadal timescales

Abstract. We describe several new ice velocity maps produced by the Greenland Ice Mapping Project (GIMP) using Landsat 8 and Copernicus Sentinel 1A/B data. We then focus on several sites where we analyse these data in conjunction with earlier data from this project, which extend back to the year 2000. At Jakobshavn Isbrae and Koge Bugt, we find good agreement when comparing results from different sensors. In a change from recent behaviour, Jakobshavn Isbrae began slowing substantially in 2017, with a midsummer peak that was even slower than some previous winter minima. Over the last decade, we identify two major slowdown events at Koge Bugt that coincide with short-term advances of the terminus. We also examined populations of glaciers in north-west and south-west Greenland to produce a record of speed-up since 2000. Collectively these glaciers continue to speed up, but there are regional differences in the timing of periods of peak speed-up. In addition, we computed trends in winter flow speed for much of the south-west margin of the ice sheet and find little in the way of statistically significant changes over the period covered by our data. Finally, although the consistency of the data is generally good over time and across sensors, our analysis indicates that substantial differences can arise in regions with high strain rates (e.g. shear margins) where sensor resolution can become a factor. For applications such as constraining model inversions, users should factor in the impact that the data's resolution has on their results.
Pancreaticoduodenectomy for an Ampullary Region Carcinoma Occurred in Annular Pancreas Coexistent with Replaced Common Hepatic Artery

Introduction. Annular pancreas is a rare congenital abnormality characterized by a ring of pancreatic tissue surrounding the descending portion of the duodenum. Annular pancreas coexisting with replaced common hepatic artery which is also a rare anatomical variation has not been reported previously. Case Presentation. A 53-year-old man visited our hospital complaining of epigastric pain. Based on radiological examinations, he was diagnosed as having pancreatitis, annular pancreas, and hepatomesenteric trunk. One month later, obstructive jaundice developed. Endoscopic examination revealed ampullary region carcinoma. We performed pancreaticoduodenectomy using the “artery-first” approach. Discussion. Both annular pancreas and common hepatic artery anomaly are rare. High-quality preoperative imaging and awareness of such rare conditions are necessary for operative safety. Although the embryological relationship between these anomalies is uncertain, the present case may suggest some relevance between the two. Conclusion. The “artery-first” approach may be a useful method for pancreaticoduodenectomy in patients who have an anatomical abnormality.
Formalizing Place in Geographic Information Systems

The past four decades have witnessed a rapid and accelerating growth in the use of computers to handle geographic information. As machines, computers require that inputs be formalized, following well-defined rules and using shared definitions of terms. This requirement has created a fundamental tension with the informal world of human discourse, and nowhere is this more apparent than over the vague concept of place. The chapter explores this tension from various perspectives: current methods of geographic representation in digital form, inherent ambiguities, the case of the gazetteer, the role of volunteered geographic information, and place as an expression of context. Examples are used to illustrate the basic principles.
Building theory in public relations: Interorganizational relationships as a public relations paradigm

This article suggests that agreement on a paradigm focus for public relations scholarship would greatly enhance the probability of productive theory development. The author argues, influenced by Th...
Kinematic viscosity measurement of granular flows via low Reynolds number cylinder drag experiment

Confined, vibration-driven grain piles exhibit fluid-like properties, in particular, predictable, non-random flow patterns, hydrodynamic modal response to vibrational forcing, and a persistent, spatially uniform tendency toward local statistical mechanical equilibrium. This paper presents a technique that combines particle image velocimetry of vibration-driven grain flow over a submerged, instrumented cylinder and measurement of the flow-induced drag force on the cylinder to determine the grain flows effective kinematic viscosity. The fundamental basis of such measurements is provided by recent work showing that high-restitution grain piles subject to low-amplitude vibration are macroscopic dynamical analogs of liquid-state molecular hydrodynamic systems. Practically, the proposed viscometric method provides a key material property, the kinematic, or equivalently, dynamic viscosity, for use in computational fluid dynamic simulations of a variety of materials processing operations that utilize vibration-driven grain flows.
Emerging Powers and Leadership Norms

Leadership is a notoriously ambiguous term in international politics, one with meanings that range from disguised hegemony or domination on the one hand to purely consensual coalition-building and the provision of focal points for collective action on the other. The term usually has positive normative content (leadership is viewed as necessary for global governance to succeed), but the form that leadership takes—the mix of instruments that are deployed, whether a single leader is necessary or collective leadership is possible—varies over time and across regions. As global leadership by the USA is called into question, the leadership norms advanced by emerging powers represent different formulas that may ultimately be transferred to the global level. Brazil in South America, Germany in Europe, India in South Asia, and China in East Asia demonstrate specific types of leadership that may coincide or clash as their roles in global governance grow in importance.
296 Frequency of intraoperative consultation for upper abdominal primary debulking surgery in advanced ovarian cancer

Objectives To gauge the learning curve of gynecologic oncologist (GOs) by measuring their need for intraoperative consultants in upper abdominal primary debulking surgery for advanced ovarian cancer (OC). Methods Patients with bulky upper abdominal disease (UAB) undergoing primary debulking surgery (PDS) for OC from 1/2001–12/2013 were included. UAB was defined as tumor >1 cm cephalad to the omentum. Extensive procedures included diaphragm resection/peritonectomy, splenectomy, distal pancreatectomy, partial liver resection, resection of tumor from the porta hepatis, partial gastrectomy, and cholecystectomy. Results Of 585 patients identified, 452 (77%) underwent UAS. The most common procedures were diaphragm resection (n=413), splenectomy (n=141), and liver resection (n=97). Over the 13-year period, the rate of patients undergoing UAS increased from 77% to 84% (p=0.019). Median number of UAS procedures was 1 (range, 1–7), remaining constant over time (p=0.129). The percentage of UAS procedures performed by consultants decreased over time, from 100% in the first quartile to 50% in the last (p Conclusions GOs who attain the learning curve perform UAS with maximal cytoreduction, with a success rate similar to that of intraoperative consultants. Including UAS in the surgical armamentarium contributes to increased rates of CGR.
Catalyzing traffic safety advancements via data linkage: Development of the New Jersey Safety and Health Outcomes (NJ-SHO) data warehouse

Objective: Our objective is to describe the development of the New Jersey Safety and Health Outcomes (NJ-SHO) data warehouse, a unique and comprehensive data source that integrates various state-level administrative databases in New Jersey to enable the field of traffic safety to address critical, high-priority research questions.Methods: We have obtained full identifiable data from the following statewide administrative databases for the state of New Jersey: (1) driver licensing database; (2) Administration Office of the Courts data on traffic-related citations; (3) police-reported crash database; (4) birth certificate data; (5) death certificate data; and (6) hospital discharge data as well as (7) childhood electronic records from New Jersey residents who were patients of the Children's Hospital of Philadelphia pediatric health care network and (8) census tract-level indicators. We undertook an iterative process to develop a linkage algorithm in LinkSolv 9.0 software using records for individuals born in select birth years (1987 and 1988) and subsequently execute the linkage for the entire study period (2004-2017). Several metrics were used to evaluate the quality of the linkage process.Results: We identified a total of 62,685,619 records and 19,247,363 distinct individuals; 10,352,998 of these individuals had more than one record brought together during the linkage process. Our evaluation of this linkage suggests that the linkage was of high quality.Conclusions: The resulting NJ-SHO data warehouse will be one of the most comprehensive and rich traffic safety data warehouses to date. The warehouse has already been utilized for numerous studies and will be fully primed to support a host of rigorous studies, both in and beyond the field of traffic safety.
MISTR : a conserved MItochondrial STress Response network revealed by signatures of evolutionary conflict.

Host-pathogen conflicts leave genetic signatures of variation in homologous host genes. Using these molecular scars as a guide, we discovered a vertebrate-specific MItochondrial STress Response circuit (MISTR). MISTR proteins are associated with electron transport chain factors and activated by stress signals such as interferon-gamma and hypoxia. Upon stress, ultraconserved miRNAs downregulate MISTR1 followed by replacement with paralogs MISTR AntiViral (MISTRAV) or MISTR Hypoxia (MISTRH), depending on the insult. While cells lacking MISTR1 are more sensitive to apoptotic triggers, cells lacking MISTRAV or expressing the poxvirus-encoded vMISTRAV exhibit resistance to the same insults. Rapid evolution signatures across primate genomes for MISTR1 and MISTRAV indicate ancient and ongoing conflicts with pathogens. MISTR proteins are also found in plants, yeasts, and an algal virus indicating ancient origins and suggesting diverse means of altering mitochondrial function under stress. The discovery of MISTR circuitry highlights the use of evolution-guided studies to reveal fundamental biological processes.
Sensory and monosaccharide analysis of drip brew coffee fractions versus brewing time.

BACKGROUND The composition of drip brew coffee versus brewing time has been chemically characterized in previous studies, and it is known that the total dissolved solids (TDS) systematically decreases with each fraction during the brew. Little information exists regarding the corresponding sensory attributes versus time, however, and it is unclear how TDS correlates with flavor profile. RESULTS Standard drip brews were fractionated into distinct samples by switching in an empty carafe every 30 s during the brew. Using a trained sensory descriptive panel, we found that most taste and flavor attributes decreased with brew time; for example, the earlier fractions were systematically more bitter and more sour than later fractions. Surprisingly, however, several flavor and taste attributes increased in time; for example, later fractions were systematically sweeter and more floral than earlier fractions. Since later fractions had lower TDS, these results indicate that perceived sweetness in drip brew coffee is negatively correlated with TDS. Mass spectrometry measurements of the monosaccharide content in the brews showed that none of the fractions had perceptible concentrations of any monosaccharide. CONCLUSION The results of the sensory analysis and the monosaccharide analysis suggest that perceptible sweetness in coffee is a consequence of masking effects and/or the presence of sweet-associated aromas and flavors. The results further suggest that unique flavor profiles could be obtained from the same coffee grounds by judicious combinations of specific fractions. © 2020 Society of Chemical Industry.
Early and late rapid torque characteristics and select physiological correlates in middle-aged and older males

Purpose The purpose of this study was to compare early and late rapid torque parameters of the plantar flexors (PFs) in middle-aged (MM) and older (OM) males, and determine the effect of normalization to peak torque (PT) and muscle cross-sectional area (CSA). Methods Twenty-nine healthy, MM (n = 14; 45 ± 2 yrs) and OM (n = 15; 65 ± 3 yrs) performed rapid, maximal isometric contractions of the PFs. PT, as well as rate of torque development and impulse during the early (0-50 ms; RTD0-50, IMP0-50) and late (100-200 ms; RTD100-200, IMP100-200) contraction phases were calculated. Torque at 50 (TQ50), 100 (TQ100), and 200 (TQ200) ms was also obtained. CSA and echo-intensity (EI) of the gastrocnemii were acquired via ultrasonography. Torque variables were normalized to PT and CSA. Rate of EMG rise (RER) for the medial gastrocnemius was calculated at 30, 50 and 75 ms. Results TQ100 (MM = 69.71 ± 16.85 vs. OM = 55.99 ± 18.54 Nm; p = 0.046), TQ200 (MM = 114.76 ± 26.79 vs. OM = 91.56 ± 28.10 Nm; p = 0.031), and IMP100-200 (MM = 4.79 ± 1.11 vs. OM = 3.83 ± 1.17 Nm·s; p = 0.032) were lower in OM. PT, TQ50, RTD0-50, IMP0-50, RTD100-200, RER, CSA, and EI were similar between groups (p > 0.05). No differences were found for normalized torque variables (p > 0.05). EI was moderately associated with normalized torque parameters only (r = -0.38 --0.45). RER, at 75 ms, was moderately correlated with early, absolute torque measures and rapid torque variables made relative to PT and CSA (r = 0.41 --0.64). Conclusion Late rapid torque parameters of the PFs were preferentially impaired in OM compared to MM, and PT as well as CSA appeared to mediate this result.
Trunk and Lower Extremity Movement Patterns, Stress Fracture Risk Factors, and Biomarkers of Bone Turnover in Military Trainees.

Context Military service members commonly sustain lower extremity stress fractures (SFx). How SFx risk factors influence bone metabolism is unknown. Understanding how SFx risk factors influence bone metabolism may help to optimize risk-mitigation strategies. Objective To determine how SFx risk factors influence bone metabolism. Design Cross-sectional study. Setting Military service academy. Patients or other participants Forty-five men (agepre = 18.56 ± 1.39 years, heightpre = 176.95 ± 7.29 cm, masspre = 77.20 ± 9.40 kg; body mass indexpre = 24.68 ± 2.87) who completed Cadet Basic Training (CBT). Individuals with neurologic or metabolic disorders were excluded. Intervention(s) We assessed SFx risk factors (independent variables) with (1) the Landing Error Scoring System (LESS), (2) self-reported injury and physical activity questionnaires, and (3) physical fitness tests. We assessed bone biomarkers (dependent variables; procollagen type I amino-terminal propeptide [PINP] and cross-linked collagen telopeptide [CTx-1]) via serum. Main outcome measure(s) A markerless motion-capture system was used to analyze trunk and lower extremity biomechanics via the LESS. Serum samples were collected post-CBT; enzyme-linked immunosorbent assays determined PINP and CTx-1 concentrations, and PINP : CTx-1 ratios were calculated. Linear regression models demonstrated associations between SFx risk factors and PINP and CTx-1 concentrations and PINP : CTx-1 ratio. Biomarker concentration mean differences with 95% confidence intervals were calculated. Significance was set a priori using α ≤ .10 for simple and α ≤ .05 for multiple regression analyses. Results The multiple regression models incorporating LESS and SFx risk factor data predicted the PINP concentration (R2 = 0.47, P = .02) and PINP : CTx-1 ratio (R2 = 0.66, P = .01). The PINP concentration was increased by foot internal rotation, trunk flexion, CBT injury, sit-up score, and pre- to post-CBT mass changes. The CTx-1 concentration was increased by heel-to-toe landing and post-CBT mass. The PINP : CTx-1 ratio was increased by foot internal rotation, lower extremity sagittal-plane displacement (inversely), CBT injury, sit-up score, and pre- to post-CBT mass changes. Conclusions Stress fracture risk factors accounted for 66% of the PINP : CTx-1 ratio variability, a potential surrogate for bone health. Our findings provide insight into how SFx risk factors influence bone health. This information can help guide SFx risk-mitigation strategies.
Dynamic Subsurface Deformation and Strain of Soft Hydrogel Interfaces Using an Embedded Speckle Pattern With 2D Digital Image Correlation

Subsurface mechanisms can greatly affect the mechanical behavior of biological materials, but observation of these mechanisms has remained elusive primarily due to unfavorable optical characteristics. Researchers attempt to overcome these limitations by performing experiments in biological mimics like hydrogels, but measurements are generally restricted due to the spatio-temporal limitations of current methods. Utilization of contemporary 3D printing techniques into soft, transparent, aqueous yield-stress materials have opened new avenues of approach to overcoming these roadblocks. By incorporating digital image correlation with such 3D printing techniques, a method is shown here that can acquire full-field deformation of a hydrogel subsurface in real-time. Briefly, the method replaces the solvent of a transparent and low polymer concentration yield-stress material with an aqueous hydrogel precursor solution, then a DIC speckle plane is 3D printed into it. This complex is then polymerized using photoinitiation thereby locking the speckle plane in place. Full-field deformation measurements are made in real-time as the embedded speckle plane (ESP) responds with the bulk to the applied load. Example results of deformation and strain fields associated with indentation, relaxation, and sliding contact experiments are shown. This method has successfully observed the subsurface mechanical response in the bulk of a hydrogel and has the potential to answer fundamental questions regarding biological material mechanical behaviors.
Paleoenvironmental changes in the Hiwegi Formation (lower Miocene) of Rusinga Island, Lake Victoria, Kenya

Abstract The lower Miocene of Rusinga Island (Lake Victoria, Kenya) is best known for its vertebrate fossil assemblage but the multiple stratigraphic intervals with well-preserved fossil leaves have received much less attention. The Hiwegi Formation has three fossil leaf-rich intervals, which span the entire formation from oldest to youngest: Kiahera Hill, R5, and R3. Here, we describe new fossil collections from Kiahera Hill and R3 and compared these floras to previous work from R5, as well as modern African floras. The oldest flora at Kiahera Hill was most similar to modern tropical rainforests or tropical seasonal forests and reconstructed as a warm and wet, closed forest. This was followed by a relatively dry and open environment at R5, which was reconstructed as a woodland to open tropical seasonal forest. The youngest flora at R3 was most similar to modern tropical seasonal forests and was reconstructed as a warm and wet spatially heterogenous forest. Floral composition of all three floras differed, but the Kiahera Hill and R3 floras were more similar to each other than either flora was to the R5 flora. The Kiahera Hill flora had few monocots or herbaceous taxa, was dominated by large leaves, and had higher species richness and greater evenness than the R3 flora. Our work, coupled with previous studies, suggests that the R3 landscape consisted of both closed forest areas and open areas with seasonal ponding. The absence of morphotypes from the R5 flora that were present in the Kiahera Hill and R3 floras provides evidence for local extirpation during the R5 time interval. Thus, this work indicates that the Hiwegi Formation on Rusinga Island samples multiple environments ranging from more closed tropical forests to more open woodlands in the Early Miocene and provides important context for the evolution and habitat preference of early apes.
Collaborations between libraries and instructional designers: a lifeline for supporting streaming video and COVID-19 induced changes on campus

The University of Wisconsin-Eau Claire (UWEC), a regional comprehensive, liberal arts university was fortunate to have laid groundwork on creating cross-departmental collaboration between the electronic resources librarian, campus copyright officer, and instructional designers before the disruption of COVID-19.
Bias in Creative Adoption Decision Points

In both creativity and innovation, individuals or collectives of individuals must evaluate creative targets and select among responses such as accept, reject, or refine. These creative adoption dec...
Effects of bromocriptine in peripartum cardiomyopathy: a systematic review and meta-analysis

Peripartum cardiomyopathy (PPCM) is a rare but potentially life-threatening form of heart failure (HF). Bromocriptine, a dopamine D2 agonist, has been used as an adjunctive treatment for PPCM with controversial benefits. A comprehensive literature search was conducted through June 2021. We included studies comparing the outcomes of PPCM with or without bromocriptine use. Pooled risk ratio (RR) with 95% confidence intervals (CI) and I2 statistics were calculated. Composite major adverse outcomes were defined by a composite of death, need for advanced HF therapies, persistent New York Heart Association (NYHA) functional class III/V, or left ventricular ejection fraction (LVEF) ≤ 35% at 6-month follow-up. LVEF recovery was defined by improvement of LVEF to more than 50%. Eight studies (two randomized-controlled, six observational) involving 593 PPCM patients were included. Bromocriptine use was associated with significantly higher survival (91.6% vs. 83.9%, RR 1.11 p = 0.02). Baseline LVEF was not significantly different between the groups. LVEF at follow-up was significantly higher in the bromocriptine group (53.3% vs. 41.8%, p < 0.001). There was no significant association between bromocriptine use and lower composite major adverse outcomes (13.7% vs. 33.3%, RR 0.60 p = 0.54) or LVEF recovery (46.9% vs. 46.8%, RR 0.94 p = 0.74). In conclusion, the addition of bromocriptine to standard HF treatment in PPCM was associated with significantly higher survival and higher LVEF improvement. No association with lower composite adverse clinical outcomes or LVEF recovery was seen. The findings, although encouraging, warrant larger randomized-controlled studies.
Artificial faces are harder to remember

We compared memory and discrimination for real and artificial faces.Artificial faces were identity-matched to real faces.Memory performance was poorer for artificial faces.Discrimination was also poorer, but this effect is smaller. Observers interact with artificial faces in a range of different settings and in many cases must remember and identify computer-generated faces. In general, however, most adults have heavily biased experience favoring real faces over synthetic faces. It is well known that face recognition abilities are affected by experience such that faces belonging to "out-groups" defined by race or age are more poorly remembered and harder to discriminate from one another than faces belonging to the "in-group". Here, we examine the extent to which artificial faces form an "out-group" in this sense when other perceptual categories are matched. We rendered synthetic faces using photographs of real human faces and compared performance in a memory task and a discrimination task across real and artificial versions of the same faces. We found that real faces were easier to remember, but only slightly more discriminable than artificial faces. Artificial faces were also equally susceptible to the well-known face inversion effect, suggesting that while these patterns are still processed by the human visual system in a face-like manner, artificial appearance does compromise the efficiency of face processing.
Patient-specific CT dosimetry calculation: a feasibility study

Current estimation of radiation dose from computed tomography (CT) scans on patients has relied on the measurement of Computed Tomography Dose Index (CTDI) in standard cylindrical phantoms, and calculations based on mathematical representations of “standard man”. Radiation dose to both adult and pediatric patients from a CT scan has been a concern, as noted in recent reports. The purpose of this study was to investigate the feasibility of adapting a radiation treatment planning system (RTPS) to provide patient-specific CT dosimetry. A radiation treatment planning system was modified to calculate patient-specific CT dose distributions, which can be represented by dose at specific points within an organ of interest, as well as organ dose-volumes (after image segmentation) for a GE Light Speed Ultra Plus CT scanner. The RTPS calculation algorithm is based on a semi-empirical, measured correction-based algorithm, which has been well established in the radiotherapy community. Digital representations of the physical phantoms (virtual phantom) were acquired with the GE CT scanner in axial mode. Thermoluminescent dosimeter (TLDs) measurements in pediatric anthropomorphic phantoms were utilized to validate the dose at specific points within organs of interest relative to RTPS calculations and Monte Carlo simulations of the same virtual phantoms (digital representation). Congruence of the calculated and measured point doses for the same physical anthropomorphic phantom geometry was used to verify the feasibility of the method. The RTPS algorithm can be extended to calculate the organ dose by calculating a dose distribution point-by-point for a designated volume. Electron Gamma Shower (EGSnrc) codes for radiation transport calculations developed by National Research Council of Canada (NRCC) were utilized to perform the Monte Carlo (MC) simulation. In general, the RTPS and MC dose calculations are within 10% of the TLD measurements for the infant and child chest scans. With respect to the dose comparisons for the head, the RTPS dose calculations are slightly higher (10%–20%) than the TLD measurements, while the MC results were within 10% of the TLD measurements. The advantage of the algebraic dose calculation engine of the RTPS is a substantially reduced computation time (minutes vs. days) relative to Monte Carlo calculations, as well as providing patient-specific dose estimation. It also provides the basis for a more elaborate reporting of dosimetric results, such as patient specific organ dose volumes after image segmentation.
An In Vitro Model of T Cell Activation by Autologous Cytomegalovirus (CMV)-Infected Human Adult Endothelial Cells: Contribution of CMV-Enhanced Endothelial ICAM-1

Cellular immunity is strongly implicated in control of CMV disease; however, many mechanistic details remain unresolved. We previously demonstrated T cell activation responses to CMV-infected allogeneic endothelial cells (EC), suggesting EC as a mediator of CMV response in the transplant recipient. We now test the hypothesis that CMV-specific T cell responses can be directly stimulated by infected EC in an environment free of potentially confounding allogeneic factors. By isolating splenic T cells and gonadal vein endothelial cells (GVEC) from individual cadaveric organ donors, we have developed an in vitro model of T cell interaction with autologous CMV-infected EC. Proliferation assays demonstrated significantly enhanced responses by CMV-seropositive donor-derived T cells cocultured with CMV-infected GVEC, as compared with those elicited by uninfected cells. Similarly, as determined by limiting dilution analysis of IL-2-producing cells, T cell response frequencies to infected GVEC were significantly greater than to uninfected EC. In contrast, responses of CMV-seronegative donor-derived T cells were minimal, regardless of CMV status of stimulator GVEC. Intriguingly, CD4 responses were observed in spite of the fact that CMV-infected EC express no HLA class II. Finally, attenuation of CMV-stimulated T cell proliferation observed in the presence of blocking Ab specific for ICAM-1 suggests a contributing role for CMV-enhanced endothelial ICAM-1 expression in the activation response. These studies demonstrate that EC can stimulate autologous T cell responses to CMV in the absence of accessory APC and suggest potentially novel mechanisms of immune activation.
Numerical simulation of the flow field and fuel sprays in an IC engine

A two-dimensional model for axisymmetric piston-cylinder configurations is developed to study the flow field in two-stroke direct-injection Diesel engines under motored conditions. The model accounts for turbulence by a two-equation model for the turbulence kinetic energy and its rate of dissipation. A discrete droplet model is used to simulate the fuel spray, and the effects of the gas phase turbulence on the droplets is considered. It is shown that a fluctuating velocity can be added to the mean droplet velocity every time step if the step is small enough. Good agreement with experimental data is found for a range of ambient pressures in Diesel engine-type microenvironments. The effects of the intake swirl angle in the spray penetration, vaporization, and mixing in a uniflow-scavenged two-stroke Diesel engine are analyzed. It is found that the swirl increases the gas phase turbulence levels and the rates of vaporization.
On Terra Firma with English

This article discusses how to write legal prose in clear, forceful, and plain English and without turgid, boring, and unclear legalisms. From the Editor: "We finish with the next installment of our 'Best of' series, this time featuring short pieces by Gerald Lebovits. Judge Lebovits . . . offers practical advice shaped by his years as a legal reader and writer. He has written many pieces worthy of inclusion here, but we’ve picked our favorite favorites."
Magnetic Resonance Imaging of Human Brain Function: Principles, Practicalities, and Possibilities

This article presents a review of functional magnetic resonance (fMR) imaging. Included are four sections that address the principles, practicalities, and potentials of fMR imaging. The current types of hemodynamic contrast available with fMR imaging, including blood volume, perfusion, and oxygenation, are discussed. Technical issues of fMR imaging, including inter pretability and sensitivity, are described, and several currently unresolved issues are addressed. Also reviewed are commonly used fMR imaging platforms and clinical and research applications.
Hypothalamic Neurons Preferentially Respond to Female Nest Coo Stimulation: Demonstration of Direct Acoustic Stimulation of Luteinizing Hormone Release

Avian vocalizations are generally understood to play a pivotal role in reproductive functions. The role of the hypothalamus in gonadotropin release in higher vertebrates including birds is well established. To date, however, a direct linkage between the neuronal processing of vocal input and the contingent luteinizing hormone (LH) response has not been demonstrated. In this study, using female ring doves, we recorded neuronal activity from hypothalamic nuclei that, as we have shown previously, receive acoustic inputs from the auditory thalamic relay. Concurrently with recording single-unit responses to stimulation with species-specific coo vocalizations, we sampled LH levels in blood from the pituitary veins. LH concentration in the plasma was significantly elevated in birds hearing species-typical coos but not in birds exposed to experimentally altered coos or white noise or in birds that received no vocal stimulation. We found two types of neurons in the preoptic and anterior hypothalamus that selectively responded to the female nest coo: excitatory units and inhibitory units. Among the excitatory neurons are units characterized by two bursts separated by a period of slow spiking or complete silence, in a pattern approximately corresponding temporally to the two-note coo. We designate them as female-nest-coo-specific units. Most neurons in the posterior hypothalamus were nonselective in their response. Female nest coo and male nest coo stimulation evoked an equal magnitude of discharge changes from responsive units in the preoptic-anterior hypothalamic area. We found, however, that the LH increment was three times greater for birds hearing female nest coos than for birds hearing male nest coos. These observations suggest that feature-detecting neurons such as the female-nest-coo-specific units are involved in gonadotropin-releasing hormone output. The present findings are consistent with the well established behavioral evidence that female nest coos mediate ovarian growth.
Use of Chemicals in Fish Management and Fish Culture

Chemicals have been used in fish management since the 1930’s and in the culture of fish since the late 1800’s. Intensive efforts were made from the 1950’s to the late 1970’s to find, develop, and register general and selective fish toxicants and aquatic herbicides, but only two selective toxicants, two general fish toxicants, two algicides, and seven aquatic herbicides were registered or are being reregistered. The expense of registration or reregistration is prohibitive for most potential products; only federal sponsorship has maintained the registration of fish toxicants. Aquatic herbicide registrations have been maintained by their registrants because there are other uses of aquatic herbicides beyond that of fishery management and culture.
Osteoid osteoma of the elbow: a diagnostic challenge.

Background: Osteoid osteoma is a painful benign neoplasm that is rarely found in the elbow region. Methods: The study included fourteen patients, and we believe that this is the largest reported series of patients with osteoid osteoma of the elbow evaluated at one institution. Most of the patients had had symptoms for a prolonged period and had had multiple invasive procedures before an accurate diagnosis was made. Although findings on physical examination generally are nonspecific and are not always accurate in localizing the lesion, plain tomograms and computed tomography scans were most helpful in identifying the nidus in the present study. Thirteen of the patients had limited motion of the elbow before the definitive diagnosis was made, and ten of these thirteen had a mean flexion contracture of 38 degrees. Results: Removal of the nidus resulted in relief of pain and improvement in the range of motion of the elbow in all fourteen patients. A persistent postoperative flexion contracture was more common in the patients who had had a previous arthrotomy of the elbow than in those who had not had that procedure. Conclusions: It is important to recognize this uncommon entity to avoid the morbidity associated with a prolonged delay in diagnosis. Because the symptoms resolve after excision of the lesion, the surgeon can avoid unnecessary soft-tissue dissection and release of the contracture.
Role of the Tet38 Efflux Pump in Staphylococcus aureus Internalization and Survival in Epithelial Cells

We previously identified the protein Tet38 as a chromosomally encoded efflux pump of Staphylococcus aureus that confers resistance to tetracycline and certain unsaturated fatty acids. Tet38 also contributes to mouse skin colonization. In this study, we discovered a novel regulator of tet38, named tetracycline regulator 21 (TetR21), that bound specifically to the tet38 promoter and repressed pump expression. A ΔtetR21 mutant showed a 5-fold increase in tet38 transcripts and an 8-fold increase in resistance to tetracycline and fatty acids. The global regulator MgrA bound to the tetR21 promoter and indirectly repressed the expression of tet38. To further assess the full role of Tet38 in S. aureus adaptability, we tested its effect on host cell invasion using A549 (lung) and HMEC-1 (heart) cell lines. We used S. aureus RN6390, its Δtet38, ΔtetR21, and ΔmgrA mutants, and a Δtet38 ΔtetR21 double mutant. After 2 h of contact, the Δtet38 mutant was internalized in 6-fold-lower numbers than RN6390 in A549 and HMEC-1 cells, and the ΔtetR21 mutant was internalized in 2-fold-higher numbers than RN6390. A slight increase of 1.5-fold in internalization was found for the ΔmgrA mutant. The growth patterns of RN6390 and the ΔmgrA and ΔtetR21 mutants within A549 cells were similar, while no growth was observed for the Δtet38 mutant. These data indicate that the Tet38 efflux pump is regulated by TetR21 and contributes to the ability of S. aureus to internalize and replicate within epithelial cells.
β-Lactamase of the Legionnaires' bacterium.

Nine strains of the Legionnaires' bacterium produced a β-lactamase that was basically a cephalosporinase, but which also had some effect on all the penicillins tested. Cefoxitin, cefuroxime, and cephalexin were resistant to the enzyme. Minimal inhibitory and bactericidal concentrations were very similar.
Development of multiplex PCR assays to identify Escherichia coli pathogenic genes in food

In order to rapidly screen for the virulence factor that produces pathogenic Escherichia coli in food, we have developed multiplex polymerase chain reaction (PCR) assays. The multiplex PCR assays detect 4 pathogenic genes of enterohaemorrhagic E. coli (EHEC), enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), and enteroinvasive E. coli (EIEC). This allows for the generation of specific fragments 150, 179, 218, 401, 465, 584, and 881 bp for VT1, ST, LT, 16S rRNA, inV, VT2, and eaeA genes, respectively. The detection limit of 3 log CFU/mL for eaeA, LT, VT1, VT2, 4 log CFU/mL for inV, 6 log CFU/mL for ST by single PCR, while 5 log CFU/mL for VT1, VT2, 6 log CFU/mL for eaeA, LT, 7 log CFU/mL for ST, inV by multiplex PCR. This optimized detection method of pathogenic E. coli can be used as supportive data to revise the microbiological analytical manuals for the Korean Food Code.
Growth and hepatospecific gene expression of human hepatoma cells in a defined medium

The production of albumin, α-fetoprotein (AFP), and α-1 antitrypsin has been compared among human hepatoma cells cultured in medium containing serum, medium containing hormones and growth factors, and a basal medium containing selenium as the only supplement. Growth is sustained in all three media, and the expression of all three proteins was maintained for over 4 mo. in the various media. However, the quantitative production of albumin and AFP were dramatically different in the three media. Two hormones, insulin and triiodothyronine, influenced the level of secreted proteins. Triiodothyronine increases the amount of secreted albumin whereas insulin at 10 μg/ml reduced the level of total secreted protein.
Predictors of Academic Self-Handicapping and Achievement: Examining Achievement Goals, Classroom Goal Structures, and Culture.

The purposes of this study were to examine the predictors and achievement consequences of academic self-handicapping and to explore cultural variations in the pursuit and effects of performance goals and perceived classroom performance goal structures. Data were collected in 2 consecutive academic years from a diverse sample of high school students (N = 675). Performance-avoidance and classroom performance goal structure were positively associated with self-handicapping, whereas performance-approach goals negatively predicted handicapping. Self-handicapping was negatively associated with achievement in English. Cultural differences in the effects of performance goals on achievement and in the effects of classroom performance goal structure on the subsequent adoption of personal performance goals were found. Implications for efforts to alter classroom goal structures are discussed.
A solvent extraction technique for determining concentrations of gold and silver in natural waters

A combination of solvent extraction and graphite furnace atomic absorption spectrophotometry was utilized to determine Au and Ag in natural water samples. The method consisted of the extraction of the two metals with methyl-isobutyl ketone (MIBK) after complexation with dithizone. The samples had previously been acidified to pH < 2 to minimize container absorption. It was necessary to utilize Teflon® containers to minimize Au loss to the container surface even at these low pH's. Detection limits for Ag were 5 and 8 pm and for Au were 2 and 3 pm for freshwater and seawater, respectively. The technique was used to analyze estuarine and continental shelf waters as well as glacial ice samples for the two metals. The results obtained by this technique are within the range reported by other workers in a similar milieu and are useful in adding to our understanding of the distribution of these two noble metals in natural waters.
Cloning of the dut (deoxyuridine triphophatase) gene of Escherichia coli

Abstract Through the molecular cloning of DNA, cells were obtained that could produce a 300-fold increased level of deoxyuridine triphosphatase (dUTPase). First, λpyrE-dut phages were constructed from restriction endonuclease fragments. They contained a segment of Escherichia coli DNA that spanned the structural genes for dUTPase (dut) and orotidylate pyrophosphorylase (pyrE). The initial isolates demonstrated poor enzyme production and impaired growth. Improved enzyme yields were then obtained from large-plaque derivatives and from mutants with partial deletions of the cloned DNA. The deletion mutants were isolated after the induction of a recombinant prophage whose DNA was too large to be packaged. Finally, a 3.3-kb segment of DNA, containing the dut gene, was transferred to plasmid vectors. The recombinants and their levels of dUTPase overproduction (relative to that of wild type cells) were as follows: a thermoinducible λpyrE-dut phage, 45-fold (10-fold for orotidylate pyrophosphorylase); a dut-ColE1 type plasmid, 15-fold; and a thermoinducible dut-λ-ColE1 chimera, 14-fold before induction and 300-fold after induction.
Wittgenstein on language and artificial intelligence: The Chinese-room thought experiment revisited

Artificial Intelligence, the study and emulation of intelligence, has brought forth a host of epistemological and ethical issues (Anderson 1964). In the wake of viable natural-language-understanding programs, the question, "What does it take to put together an understanding program?" has superseded the hypothetical query, "What if machines can think?" Philosophers who have been concerned with these issues have adopted two different points of view: (1) programs will never be able to emulate human intelligence, mainly because they lack con sciousness and intentionality (Scriven 1953; Dreyfus 1979; Searle 1980); (2) programs already manifest intelligent behavior in limited domains and are potentially able to deal with larger domains in due course (Hofstadter 1980, 680; Schank 1975, 4; Winograd 1972, 2). Although both sides agree that the computer is a useful tool for simulation of behavior, the argument ensues over the issue if programs can go beyond mere imitation of human behavior, if they can be creative, if they can think. This paper investigates these questions from a Wittgensteinian point of view. Wittgenstein's philosophy does not seem to be in the least amenable to "thinking" or "understanding" programs. It is centered around the human being, thus decidedly anthropocentric; along these lines, Witt genstein's denial of credibility of "thinking" programs is categorical: "But machines can't think" (PI, 360). Therefore, it is surprising that he is considered to be the forerunner of "the concerns of modern AI" (Wilks 1976, 222). Even more problematic is the use of quotes from Wittgenstein to support advocates of "strong" AI (i.e., people who believe that a program is a mind) (Searle 1980), as well as contenders