cssi/SciSciGPT-SciSciNet huggingface dataset:
THE DIRECT ANTERIOR APPROACH IS ADVANTAGEOUS IN TREATING DISPLACED FEMORAL NECK FRACTURES WITH ARTHROPLASTY

Introduction Prosthetic replacement remains the treatment of choice for displaced femoral neck fractures in the elderly population, with recent literature demonstrating significant functional benefits of total hip arthroplasty (THA) over hemiarthroplasty. Yet the fracture population also has historically high rates of early postoperative instability when treated with THA. The direct anterior approach (DAA) may offer the potential to decrease the risk of postoperative instability in this high-risk population by maintaining posterior anatomic structures. The addition of intraoperative fluoroscopy can improve precision in component placement and overcome limitations on preoperative planning due to poor preoperative radiographs performed in the emergency setting. Methods We retrospectively reviewed clinical and radiographic outcomes of 113 consecutive patients with displaced femoral neck fractures treated by two surgeons over a five-year period. All underwent surgery via the DAA using fluoroscopic guidance, and were allowed immediate postoperative weight bearing without any hip precautions or restrictions. Charts were reviewed for relevant complications, while radiographs were reviewed for component positioning, sizing, and leg length discrepancy. Mean follow-up was 8.9 months. Results Mean age was 79.3 years (range, 42 to 101), 73% of patients were women, and mean BMI was 22.6 kg/m 2 . Ninety patients (80%) received THA while 23 (20%) received unipolar or bipolar hemiarthroplasty. Mean acetabular anteversion was 15.0 degrees (range, 4 to 24) and mean abduction was 39.2 degrees (range, 27 to 51) with 95% of acetabular components in the combined safe zone as described by Lewinnek. Mean radiographic leg-length difference was +2.2 mm (range, −4.9 to +8.8mm). There was no femoral stem subsidence of more than 2mm. Only one patient (0.9%) dislocated postoperatively, who was eventually constrained for recurrent posterior instability 3 months following surgery. Delayed wound healing (6.1%) was the most common postoperative complication. Conclusions The direct anterior approach allows a safe, effective, and reproducible approach for treatment of displaced femoral neck fractures, with very low rate of early postoperative instability compared to historical controls. The use of intraoperative fluoroscopy allows excellent component positioning, sizing, and restoration of leg length in spite of inconsistent preoperative radiographs.
The Stochastic X-Ray Variability of the Accreting Millisecond Pulsar MAXI J0911-655

In this work, I report on the stochastic X-ray variability of the 340 hertz accreting millisecond pulsar MAXI J0911-655. Analyzing pointed observations of the XMM-Newton and NuSTAR observatories, I find that the source shows broad band-limited stochastic variability in the 0.01-10 hertz range with a total fractional variability of approximately 24 percent root mean square timing residuals in the 0.4 to 3 kiloelectronvolt energy band that increases to approximately 40 percent root mean square timing residuals in the 3 to 10 kiloelectronvolt band. Additionally, a pair of harmonically related quasi-periodic oscillations (QPOs) are discovered. The fundamental frequency of this harmonic pair is observed between frequencies of 62 and 146 megahertz. Like the band-limited noise, the amplitudes of the QPOs show a steep increase as a function of energy; this suggests that they share a similar origin, likely the inner accretion flow. Based on their energy dependence and frequency relation with respect to the noise terms, the QPOs are identified as low-frequency oscillations and discussed in terms of the Lense-Thirring precession model.
A WEAR- AND HIGHLY OXIDATION-RESISTANT CHEMICALLY CROSS-LINKED UHMWPE WITH IMPROVED TOUGHNESS

Introduction Radiation cross-linked UHMWPE is preferred in total hip replacements due to its wear resistance [1]. In total knees, where stresses are higher, there is concern of fatigue damage [2]. Antioxidant stabilization of radiation cross-linked UHMWPE by blending vitamin E into the polymer powder was recently introduced [3]. Vitamin E greatly hinders radiation cross-linking in UHMWPE [4]. In contrast peroxide cross-linking of UHMWPE is less sensitive to vitamin E concentration [5]. In addition, exposing UHMWPE to around 300°C, increases its toughness by inducing controlled chain scission and enhanced intergranular diffusion of chains, simultaneously [6]. We present a chemically cross-linked UHMWPE with high vitamin E content and improved toughness by high temperature melting. Methods and Materials Medical grade GUR1050 UHMWPE was blended with vitamin E and with 2,5-Di(tert-butylperoxy)-2,5-dimethyl-3-hexyne or P130 (0.5% Vitamin-E and 0.9% P130). The mixed powder was consolidated into pucks. The pucks were melted for 5 hours in nitrogen at 300, 310 and 320°C. One set of pucks melted at 310°C was accelerated aged at 70°C at 5 atm. oxygen for 2 weeks. Tensile mechanical properties were determined using ASTM D638. Izod impact toughness was determined using ASTM D256 and F648. Wear rate was determined using a bidirectional pin-on-disc (POD) tester with cylindrical pins of UHMWPE against polished CoCr discs in undiluted, preserved bovine serum. Results The vinyl index increased as a function of temperature (Fig 1a). Cross-link density steadily decreased and impact strength increased with increasing vinyl index (Fig 1b). The ultimate tensile strength (UTS) was not affected by HTM (Table 2). Impact strength was significantly improved for all treatment temperatures (P<0.05) and wear was significantly increased only for the sample melted at 320°C (Table 2) Discussion High temperature melting (HTM) was shown to increase toughness of UHMWPEs presumably due to controlled chain scissioning and increased intergranular diffusion of chains [6]. For radiation cross-linked UHMWPE, it was shown that an increase in elongation-at-break and impact strength could be obtained without sacrificing wear resistance up to an elongation of about 500% [7]. This vitamin E-blended, peroxide cross-linked, high temperature melted UHMWPE has very high oxidation resistance due to its high antioxidant content, high wear resistance due to cross-linking and much improved toughness, representing an optimum joint replacement surface. For figures/tables, please contact authors directly.
All-Pay Auctions with Ties

We study the two-player, complete information all-pay auction in which a tie ensues if neither player outbids the other by more than a given amount. In the event of a tie, each player receives an identical fraction of the winning prize. Players thus engage in two margins of competition: losing versus tying, and tying versus winning. Two pertinent parameters are the margin required for victory and the value of tying relative to winning. We fully characterize the set of Nash equilibria for the entire parameter space. For much of the parameter space, there is a unique Nash equilibrium which is also symmetric. Equilibria typically involve randomizing over multiple disjoint intervals, so that in essence players randomize between attempting to tie and attempting to win. In equilibrium, expected bids and payoffs are non-monotonic in both the margin required for victory and the relative value of tying.
Dynamic watermarking for general LTI systems

Detecting attacks in control systems is an important aspect of designing secure and resilient control systems. Recently, a dynamic watermarking approach was proposed for detecting malicious sensor attacks for SISO LTI systems with partial state observations and MIMO LTI systems with a full rank input matrix and full state observations; however, these previous approaches cannot be applied to general LTI systems that are MIMO and have partial state observations. This paper designs a dynamic watermarking approach for detecting malicious sensor attacks for general LTI systems, and we provide a new set of asymptotic and statistical tests. We prove these tests can detect attacks that follow a specified attack model (more general than replay attacks), and we also show that these tests simplify to existing tests when the system is SISO or has full rank input matrix and full state observations. The benefit of our approach is demonstrated with a simulation analysis of detecting sensor attacks in autonomous vehicles. Our approach can distinguish between sensor attacks and wind disturbance (through an internal model principle framework), whereas improperly designed tests cannot distinguish between sensor attacks and wind disturbance.
Cluster-randomized trial of a mobile phone personalized behavioral intervention for blood glucose control

OBJECTIVE To test whether adding mobile application coaching and patient/provider web portals to community primary care compared with standard diabetes management would reduce glycated hemoglobin levels in patients with type 2 diabetes. RESEARCH DESIGN AND METHODS A cluster-randomized clinical trial, the Mobile Diabetes Intervention Study, randomly assigned 26 primary care practices to one of three stepped treatment groups or a control group (usual care). A total of 163 patients were enrolled and included in analysis. The primary outcome was change in glycated hemoglobin levels over a 1-year treatment period. Secondary outcomes were changes in patient-reported diabetes symptoms, diabetes distress, depression, and other clinical (blood pressure) and laboratory (lipid) values. Maximal treatment was a mobile- and web-based self-management patient coaching system and provider decision support. Patients received automated, real-time educational and behavioral messaging in response to individually analyzed blood glucose values, diabetes medications, and lifestyle behaviors communicated by mobile phone. Providers received quarterly reports summarizing patient’s glycemic control, diabetes medication management, lifestyle behaviors, and evidence-based treatment options. RESULTS The mean declines in glycated hemoglobin were 1.9% in the maximal treatment group and 0.7% in the usual care group, a difference of 1.2% ( P = 0.001) over 12 months. Appreciable differences were not observed between groups for patient-reported diabetes distress, depression, diabetes symptoms, or blood pressure and lipid levels (all P > 0.05). CONCLUSIONS The combination of behavioral mobile coaching with blood glucose data, lifestyle behaviors, and patient self-management data individually analyzed and presented with evidence-based guidelines to providers substantially reduced glycated hemoglobin levels over 1 year.
Plasma membrane association facilitates conformational changes in the Marburg virus protein VP40 dimer

Filovirus infections cause hemorrhagic fever in humans and non-human primates that often results in high fatality rates. The Marburg virus is a lipid-enveloped virus from the Filoviridae family and is closely related to the Ebola virus. The viral matrix layer underneath the lipid envelope is formed by the matrix protein VP40 (VP40), which is also involved in other functions during the viral life-cycle. As in the Ebola virus VP40 (eVP40), the recently determined X-ray crystal structure of the Marburg virus VP40 (mVP40) features loops containing cationic residues that form a lipid binding basic patch. However, the mVP40 basic patch is significantly flatter with a more extended surface than in eVP40, suggesting the possibility of differences in the plasma membrane interactions and phospholipid specificity between the VP40 dimers. In this paper, we report on molecular dynamics simulations that investigate the roles of various residues and lipid types in PM association as well as the conformational changes of the mVP40 dimer facilitated by membrane association. We compared the structural changes of the mVP40 dimer with the mVP40 dimer in both lipid free and membrane associated conditions. Despite the significant structural differences in the crystal structure, the Marburg VP40 dimer is found to adopt a configuration very similar to the Ebola VP40 dimer after associating with the membrane. This conformational rearrangement upon lipid binding allows Marburg VP40 to localize and stabilize at the membrane surface in a manner similar to the Ebola VP40 dimer. Consideration of the structural information in its lipid-interacting condition may be important in targeting mVP40 for novel drugs to inhibit viral budding from the plasma membrane.
Ontological Annotation with WordNet.

Semantic Web applications require robust and accurate annotation tools that are capable of automating the assignment of ontological classes to words in naturally occurring text (ontological annotation). Most current ontologies do not include rich lexical databases and are therefore not easily integrated with word sense disambiguation algorithms that are needed to automate ontological annotation. WordNet provides a potentially ideal solution to this problem as it offers a highly structured lexical conceptual representation that has been extensively used to develop word sense disambiguation algorithms. However, WordNet has not been designed as an ontology, and while it can be easily turned into one, the result of doing this would present users with serious practical limitations due to the great number of concepts (synonym sets) it contains. Moreover, mapping WordNet to an existing ontology may be difficult and requires substantial labor. We propose to overcome these limitations by developing an analytical platform that (1) provides a WordNet-based ontology offering a manageable and yet comprehensive set of concept classes, (2) leverages the lexical richness of WordNet to give an extensive characterization of concept class in terms of lexical instances, and (3) integrates a class recognition algorithm that automates the assignment of concept classes to words in naturally occurring text. The ensuing framework makes available an ontological annotation platform that can be effectively integrated with intelligence analysis systems to facilitate evidence marshaling and sustain the creation and validation of inference models.
Frequency domain identification of wiener models

Abstract This paper discusses Wiener model identifications in frequency domain using the sampled data, By exploring the fundamental frequency and harmonies generated by the unknown nonlinearity, we propose a frequency domain approach and show its convergence for both the linear and nonlinear subsystems in the presence of noise. No a priori knowledge of the structure of the non linearity is required and the linear part can be nonparametric.
Biochemical Underpinnings of Immune Cell Metabolic Phenotypes

The metabolism of immune cells affects their function and influences host immunity. This review explores how immune cell metabolic phenotypes reflect biochemical dependencies and highlights evidence that both the metabolic state of immune cells and nutrient availability can alter immune responses. The central importance of oxygen, energetics, and redox homeostasis in immune cell metabolism, and how these factors are reflected in different metabolic phenotypes, is also discussed. Linking immune cell metabolic phenotype to effector functions is important to understand how altering metabolism can impact the way in which immune cells meet their metabolic demands and affect the immune response in various disease contexts.
Role of Anisotropic Strength and Stiffness in Governing the Initiation and Propagation of Yielding in Polycrystalline Solids

The ratio of directional strength-to-stiffness is important in governing the relative order in which individual crystals within a polycrystalline aggregate will yield as the aggregate is loaded. In this paper, a strength-to-stiffness parameter is formulated for multiaxial loading that extends a similar parameter developed specifically for uniaxial loading. Building on the principle of strength-to-stiffness, a methodology for predicting the macroscopic stresses at which elements in a finite element mesh yield is developed. This analysis uses elastic strain data from one increment of a purely elastic finite element simulation to make the prediction, given knowledge of the single-crystal yield surface. Simulations of austenitic stainless steel AL6XN are used to demonstrate the effectiveness of the strength-to-stiffness parameter and yield prediction methodology.
A tax dead on arrival: classical liberalism, inheritance, and social mobility

Historically, it is safe to say that very few laws did as much to stoke inequality as laws touching descents and hereditary transmissions. This paper attempts to see if the classical liberal tradit...
GENE-10. H3K27ME3-MEDIATED SILENCING TUMOR SUPPRESSORS SUPPORTS THE PROLIFERATION OF PEDIATRIC BRAIN TUMOR CELLS HARBORING THE H3.3K27M MUTATION.

The lysine 27 to methionine mutation of histone H3.3 (H3.3K27M) is detected in over 75% of diffuse intrinsic pontine glioma (DIPG). The H3.3K27M mutant proteins inhibit H3K27 methyltransferase complex PRC2, resulting in a global reduction of tri-methylation of H3K27 (H3K27me3). Paradoxically, high levels of H3K27me3 were also detected at hundreds of genomic loci. However, it is not known how and why H3K27me3 is redistributed in DIPG cells. Here we show that lower levels of H3.3K27M mutant proteins at some genomic loci contribute to the retention of H3K27me3 peaks. But more importantly, Jarid2, a PRC2-associated protein, strongly correlates the presence of H3K27me3 and relieves the H3.3K27M-mediated inhibition in vivo and in vitro. Furthermore, we show that H3K27me3-mediated silencing of tumor suppressor gene Wilms Tumor 1 (WT1) supports the proliferation of DIPG cells and reaction of WT1 inhibits DIPG proliferation. Together, these studies reveal mechanisms whereby H3K27me3 is retained in the environment of global loss of this mark, and how persistence of this mark contributes to DIPG tumorigenesis.
“I’d rather be dead than disabled”—the ableist conflation and the meanings of disability

ABSTRACTDespite being assailed for decades by disability activists and disability studies scholars spanning the humanities and social sciences, the medical model of disability—which conceptualizes disability as an individual tragedy or misfortune due to genetic or environmental insult—still today structures many cases of patient–practitioner communication. Synthesizing and recasting work done across critical disability studies and philosophy of disability, I argue that the reason the medical model of disability remains so gallingly entrenched is due to what I call the “ableist conflation” of disability with pain and suffering. In an effort to better equip healthcare practitioners and those invested in health communication to challenge disability stigma, discrimination, and oppression, I lay out the logic of the ableist conflation and interrogate examples of its use. I argue that insofar as the semiosis of pain and suffering is structured by the lived experience of unwelcome bodily transition or variation,...
Citizen Participation: A Critical Look at the Democratic Adequacy of Government Consultations

Consultation procedures are used increasingly in the United Kingdom and elsewhere. This account looks critically at consultation as presently practiced, and suggests that consulters and consultees need to do much more to ensure both the participatory validity and democratic value of such exercises. The possibility of a “right to be consulted” is examined. Some ideas from a governmentality perspective are developed, using the growth of localism as an example, to suggest that consultation is often a very structured interaction: the actual operation of participation mechanisms may not always create a space for an equal exchange between official and participant views. Examples of best practice in consultation are examined before consideration is given to recent case law from the UK seeking to establish basic ground rules for how consultations should be organized. Finally the promise of consultation to reinvigorate democracy is evaluated and weighed against the correlative risk of “participatory disempowerment".
Serum Hormone Levels Among Patients with Prostatic Carcinoma or Benign Prostatic Hyperplasia and Clinic Controls

This study sought to identify differences in serum hormone levels between prostatic cancer (Cap) patients, benign prostatic hyperplasia (BPH) patients, and clinic controls (CC). Serum testosterone, estradiol, and prolactin values were obtained from 35 Cap, 42 BPH, and 161 CC patients attending a single medical center between January 1984 and April 1985. Relative risk estimates adjusted for age and race were calculated to compare hormone values between each case group and the CC. The distributions of hormone values and the testosterone to estradiol (T/E) ratios were grouped into thirds with the lowest third forming the reference category. The relative risk estimates for BPH in the middle and high thirds of testosterone were greater than unity (1.26 and 2.10, respectively), whereas the relative risk estimates in the middle and high thirds of estradiol were less than unity (0.63 and 0.35, respectively). For the middle and high thirds of the T/E ratio, the relative risk estimates for BPH showed statistically significant three- to fourfold increases. Modest depression of serum testosterone and estradiol was noted for CaP patients compared to CC, although the differences were not statistically significant. This depression was interpreted to be a likely result of the malignant process rather than a cause of it, whereas the development of clinically evident BPH was felt to be a biologically plausible response to an elevated T/E ratio.
Unheard voices: The challenge of inducing women’s civic speech

Deliberative institutions have gained popularity in the developing world as a means by which to make governance more inclusive and responsive to local needs. However, a growing body of evidence suggests that persistent gender inequality may limit women's ability to participate actively and influence outcomes in these forums. In response, policy makers have tried to induce women's participation by leveraging the group-based format of self-help groups, which can build women's social capital and develop their sense of political efficacy and identity. This paper evaluates the impact of one such intervention, known as the Pudhu Vaazhvu Project, on women's civic participation in rural Tamil Nadu. Using text-as-data methods on a matched sample of transcripts from village assembly meetings, the analysis finds that the Pudhu Vaazhvu Project significantly increases women's participation in the gram sabha along several dimensions -- meeting attendance, propensity to speak, and the length of floor time they enjoy. Although women in the Pudhu Vaazhvu Project villages enjoy greater voice, the study finds no evidence that they are more likely than women in control villages to drive the broader conversational agenda or elicit a relevant response from government officials.
Open Treatment of Peritonitis: An Argument Against

We conclude that open management of advanced peritonitis may provide improved drainage, but it does so at an intolerable cost of increased mechanical lesions, namely, fistulas. The theories of animal investigators need to be tested at the bedside and in the hospital operating room before this idea receives any further consideration. "Semi-open" treatment has been widely and usefully used by many surgeons. Unfortunately, it provides less than total drainage and has its own set of major problems related to mesh removal and/or ultimate wound closure. We think that a better alternative for future controlled study is the sporadically espoused but impressively effective continuing peritoneal lavage with dilute solutions of antibiotics.
Multi-Area Interchange Scheduling Under Uncertainty

The problem of multi-area interchange scheduling under system uncertainty is considered in this paper. A new scheduling technique is proposed for a multi-proxy bus system based on stochastic optimization that captures uncertainty in renewable generation and stochastic load. In particular, the proposed algorithm iteratively optimizes interface flows using multidimensional demand and supply functions. Optimality and convergence are guaranteed for both synchronous and asynchronous scheduling under nominal assumptions.
Experimental fabrication and nondestructive testing of carbon fiber beams for delaminations using embedded Terfenol-D particles

The experimental results for damage sensing using embedded magnetostrictive particles as sensors in carbon fiber–reinforced polymer laminates are presented. Laminates constructed of varying counts ...
Space-Time Supersymmetry in Compactified String Theory and Superconformal Models

Space-time supersymmetric compactified string theories which obey spin and statistics, tachyon free, and modular invariant, are constructed by compactifying on arbitrary sums of N = 2 minimal superconformal models. Heterotic-type compactifications with realistic phenomenology are described. The theories offer a considerably simpler structure than standard supersymmetric compactifications, gauge groups which contain E6 with chiral fermions in realistic grand unification representations, and no free parameters. Also given are the partition functions and the classification of modular invariants for the N = 2 minimal superconformal models.
Off-axis short GRBs from structured jets as counterparts to GW events

Binary neutron star mergers are considered to be the most favorable sources that produce electromagnetic (EM) signals associated with gravitational waves (GWs). These mergers are the likely progenitors of short duration gamma-ray bursts (GRBs). The brief gamma-ray emission (the "prompt GRB" emission) is produced by ultra-relativistic jets, as a result, this emission is strongly beamed over a small solid angle along the jet. It is estimated to be a decade or more before a short GRB jet within the LIGO volume points along our line of sight. For this reason, the study of the prompt signal as an EM counterpart to GW events has been sparse. We argue that for a realistic jet model, one whose luminosity and Lorentz factor vary smoothly with angle, the prompt signal can be detected for a significantly broader range of viewing angles. This can lead to a new type of EM counterpart, an "off-axis" short GRB. Our estimates and simulations show that it is feasible to detect these signals with the aid of the temporal coincidence from a LIGO trigger, even if the observer is substantially misaligned with respect to the jet.
Characterizing the Nature and Dynamics of Tor Exit Blocking

Facing undesired traffic from the Tor anonymity network, online service providers discriminate against Tor users. In this study we characterize the extent of discrimination faced by Tor users and the nature of undesired traffic exiting from the Tor network - a task complicated by Tor's need to maintain user anonymity. We leverage multiple independent data sources: email complaints sent to exit operators, commercial threat intelligence, webpage crawls via Tor, and privacy-sensitive measurements of our own Tor exit nodes to address this challenge. We develop methods for classifying email complaints sent to abuse contacts and an interactive crawler to find subtle forms of discrimination on the Web, and deploy our own exits in various configurations to understand which are prone to discrimination. We find that conservative exit policies are ineffective in preventing the blacklisting of exit relays. However, a majority of the attacks originating from Tor generate high traffic volume, suggesting the possibility of detection and prevention without violating Tor users' privacy. Based on work published at [1]. [1]: Rachee Singh, Rishab Nithyanand, Sadia Afroz, Paul Pearce, Michael Carl Tschantz, Phillipa Gill, and Vern Paxson.
Extended Synaptotagmin Localizes to Presynaptic ER and Promotes Neurotransmission and Synaptic Growth in Drosophila

The endoplasmic reticulum (ER) is an extensive organelle in neurons with important roles at synapses including the regulation of cytosolic Ca2+, neurotransmission, lipid metabolism, and membrane trafficking. Despite intriguing evidence for these crucial functions, how the presynaptic ER influences synaptic physiology remains enigmatic. To gain insight into this question, we have generated and characterized mutations in the single extended synaptotagmin (Esyt) ortholog in Drosophila melanogaster Esyts are evolutionarily conserved ER proteins with Ca2+-sensing domains that have recently been shown to orchestrate membrane tethering and lipid exchange between the ER and plasma membrane. We first demonstrate that Esyt localizes to presynaptic ER structures at the neuromuscular junction. Next, we show that synaptic growth, structure, and homeostatic plasticity are surprisingly unperturbed at synapses lacking Esyt expression. However, neurotransmission is reduced in Esyt mutants, consistent with a presynaptic role in promoting neurotransmitter release. Finally, neuronal overexpression of Esyt enhances synaptic growth and the sustainment of the vesicle pool during intense activity, suggesting that increased Esyt levels may modulate the membrane trafficking and/or resting Ca2+ pathways that control synapse extension. Thus, we identify Esyt as a presynaptic ER protein that can promote neurotransmission and synaptic growth, revealing the first in vivo neuronal functions of this conserved gene family.
A Dose of Managed Care: Controlling Drug Spending in Medicaid

We study the effect of privatizing Medicaid drug benefits on drug prices and utilization. Drug spending would decrease by 21.3 percent if private insurers administered all drug benefits. One-third of the decrease is driven by private insurers' ability to negotiate prices with pharmacies. The remaining two-thirds is driven by the greater use of lower cost drugs, such as generics, and is only realized in states that give private insurers the flexibility to design drug benefits. Privatization does not reduce prescriptions per enrollee and spending cuts are smaller for drugs that lower medical spending.
A Hyperthermophilic Phage Decoration Protein Suggests Common Evolutionary Origin with Herpesvirus Triplex Proteins and an Anti-CRISPR Protein.

Summary Virus capsids are protein shells that protect the viral genome from environmental assaults, while maintaining the high internal pressure of the tightly packaged genome. To elucidate how capsids maintain stability under harsh conditions, we investigated the capsid components of the hyperthermophilic phage P74-26. We determined the structure of capsid protein gp87 and show that it has the same fold as decoration proteins in many other phages, despite lacking significant sequence homology. We also find that gp87 is significantly more stable than mesophilic homologs. Our analysis of the gp87 structure reveals that the core "β tulip" domain is conserved in trimeric capsid components across numerous double-stranded DNA viruses, including Herpesviruses. Moreover, this β barrel domain is found in anti-CRISPR protein AcrIIC1, suggesting a mechanism for the evolution of this Cas9 inhibitor. Our work illustrates the principles for increased stability of gp87, and extends the evolutionary reach of the β tulip domain.
Building resilience to natural disasters: An application to small developing states

Abstract We present a dynamic small open economy model to explore the macroeconomic impact of a major natural disaster. In addition to permanent damages to public and private capital, the disaster causes temporary losses of productivity, inefficiencies during the reconstruction process, and damages to the sovereign's creditworthiness. We use the model to study the debt sustainability concerns that arise from the need to fully rebuild public infrastructure over the medium term and analyze the feasibility of ex ante policies, such as building adaptation infrastructure and fiscal buffers, and contrast these policies with the post-disaster support provided by donors. Investing in resilient infrastructure may prove useful, in particular if it is viewed as complementary to standard infrastructure, because it raises the marginal product of private capital, crowding in private investment, while helping withstand the impact of the natural disaster. In an application to Vanuatu, we find that donors should provide an additional 50% of pre-cyclone GDP in grants to be spent over the following 15 years to ensure public debt remains sustainable following Cyclone Pam. Helping the government build resilience on the other hand, reduces the risk of debt distress and at lower cost for donors.
Aggregate Hardware Impairments Over Mixed RF/FSO Relaying Systems With Outdated CSI

In this paper, we propose a dual-hop radio-frequency (RF)/free-space optical system with multiple relays employing the decode-and-forward and amplify-and-forward with a fixed gain relaying scheme. The RF channels are subject to a Rayleigh distribution while the optical links experience a unified fading model emcopassing the atmospheric turbulence that follows the Malaga distribution (or also called the $\mathcal {M}$ -distribution), the atmospheric path loss, and the pointing error. Partial relay selection with outdated channel state information is proposed to select the candidate relay to forward the signal to the destination. At the reception, the detection of the signal can be achieved following either heterodyne or intensity modulation and direct detection. Many previous attempts neglected the impact of the hardware impairments and assumed ideal hardware. This assumption makes sense for low data rate systems but it would no longer be valid for high data rate systems. In this paper, we propose a general model of hardware impairment to get insight into quantifying its effects on the system performance. We will demonstrate that the hardware impairments have small impact on the system performance for low signal-to-noise ratio (SNR), but it can be destructive at high SNR values. Furthermore, analytical expressions and upper bounds are derived for the outage probability and ergodic capacity while the symbol error probability is obtained through the numerical integration method. Capitalizing on these metrics, we also derive the high SNR asymptotes to get valuable insight into the system gains, such as the diversity and the coding gains. Finally, analytical and numerical results are presented and validated by the Monte Carlo simulation.
As Extracellular Glutamine Levels Decline, Asparagine Becomes an Essential Amino Acid

When mammalian cells are deprived of glutamine, exogenous asparagine rescues cell survival and growth. Here we report that this rescue results from use of asparagine in protein synthesis. All mammalian cell lines tested lacked cytosolic asparaginase activity and could not utilize asparagine to produce other amino acids or biosynthetic intermediates. Instead, most glutamine-deprived cell lines are capable of sufficient glutamine synthesis to maintain essential amino acid uptake and production of glutamine-dependent biosynthetic precursors, with the exception of asparagine. While experimental introduction of cytosolic asparaginase could enhance the synthesis of glutamine and increase tricarboxylic acid cycle anaplerosis and the synthesis of nucleotide precursors, cytosolic asparaginase suppressed the growth and survival of cells in glutamine-depleted medium in vitro and severely compromised the in vivo growth of tumor xenografts. These results suggest that the lack of asparaginase activity represents an evolutionary adaptation to allow mammalian cells to survive pathophysiologic variations in extracellular glutamine.
Surface-plasmon opto-magnetic field enhancement for all-optical magnetization switching

The demand for faster magnetization switching speeds and lower energy consumption has driven the field of spintronics in recent years. The magnetic tunnel junction is the most developed spintronic memory device in which the magnetization of the information storage layer is switched by spin-transfer-torque or spin-orbit torque interactions. Whereas these novel spin-torque interactions exemplify the potential of electron-spin-based devices and memory, the switching speed is limited to the ns regime by the precessional motion of the magnetization. All-optical magnetization switching, based on the inverse Faraday effect, has been shown to be an attractive method for achieving magnetization switching at sub-ps speeds. Successful magnetization reversal in thin films has been demonstrated by using circularly polarized light. However, a method for all-optical switching of on-chip nanomagnets in high density memory modules has not been described. In this work we propose to use plasmonics, with CMOS compatible plasmonic materials, to achieve on-chip magnetization reversal in nanomagnets. Plasmonics allows light to be confined in dimensions much smaller than the diffraction limit of light. This in turn yields higher localized electromagnetic field intensities. In this work, through simulations, we show that by using localized surface plasmon resonances, it is possible to couple light to nanomagnets and achieve significantly higher opto-magnetic field values in comparison to free space light excitation.
RAB-35 and ARF-6 GTPases Mediate Engulfment and Clearance Following Linker Cell-Type Death.

Summary Clearance of dying cells is essential for development and homeostasis. Conserved genes mediate apoptotic cell removal, but whether these genes control non-apoptotic cell removal is a major open question. Linker cell-type death (LCD) is a prevalent non-apoptotic developmental cell death process with features conserved from C. elegans to vertebrates. Using microfluidics-based long-term in vivo imaging, we show that unlike apoptotic cells, the C. elegans linker cell, which dies by LCD, is competitively phagocytosed by two neighboring cells, resulting in cell splitting. Subsequent cell elimination does not require apoptotic engulfment genes. Rather, we find that RAB-35 GTPase is a key coordinator of competitive phagocytosis onset and cell degradation. RAB-35 binds CNT-1, an ARF-6 GTPase activating protein, and removes ARF-6, a degradation inhibitor, from phagosome membranes. This facilitates phosphatidylinositol-4,5-bisphosphate removal from phagosome membranes, promoting phagolysosome maturation. Our studies suggest that RAB-35 and ARF-6 drive a conserved program eliminating cells dying by LCD.
English Competition Law Before 1900

English competition law before 1900 developed over many centuries and reflected changes in political conditions, economic theories and social values. It mirrored the historical movements in England...
Asymmetric histone incorporation during DNA replication in Drosophila male germline stem cells

Many stem cells undergo asymmetric division to produce both a self-renewing stem cell and a differentiating daughter cell. We previously reported that in male Drosophila germline stem cells (GSCs), preexisting (old) histone H3 is inherited to the self-renewed stem cell, whereas the differentiating daughter is enriched with newly synthesized (new) H3. However, the molecular mechanisms that establish asymmetric H3 distribution on sister chromatids were unclear. Here, we show that histone H4 is likewise inherited asymmetrically, while histones H2A and H2B are both inherited symmetrically. We hypothesize that the asymmetric distribution of H3 and H4 at sister chromatids is established during DNA replication. To directly visualize histone incorporation pattern on replicating sister chromatids, we develop a chromatin fiber technology with differential labeling of old versus new histones. We find spatially separable distributions of old and new H3 on isolated replicating sister chromatids. By contrast, old and new H2A displayed symmetric distribution on replicating sister chromatids. Furthermore, co-localization studies on chromatin fibers and proximity ligation assays on intact nuclei reveal that old H3 are preferentially retained by the leading strand while new H3 preferentially associate with the lagging strand. Finally, using a sequential nucleoside analog incorporation assay, we detect significantly increased unidirectional DNA replication on chromatin fibers from germline compared to somatic cells. Together, the unidirectionality of fork movement coupled with the strandness of histone incorporation could explain the asymmetry between old and new H3 on replicated sister chromatids. In summary, these results indicate that the spatial and temporal asymmetries inherent to DNA replication process may serve to bias histone incorporation, suggesting an unappreciated role for DNA replication in asymmetrically dividing cells.
Myocardial Polyploidization Creates a Barrier to Heart Regeneration in Zebrafish.

Summary Correlative evidence suggests that polyploidization of heart muscle, which occurs naturally in post-natal mammals, creates a barrier to heart regeneration. Here, we move beyond a correlation by demonstrating that experimental polyploidization of zebrafish cardiomyocytes is sufficient to suppress their proliferative potential during regeneration. Initially, we determined that zebrafish myocardium becomes susceptible to polyploidization upon transient cytokinesis inhibition mediated by dominant-negative Ect2. Using a transgenic strategy, we generated adult animals containing mosaic hearts composed of differentially labeled diploid and polyploid-enriched cardiomyocyte populations. Diploid cardiomyocytes outcompeted their polyploid neighbors in producing regenerated heart muscle. Moreover, hearts composed of equivalent proportions of diploid and polyploid cardiomyocytes failed to regenerate altogether, demonstrating that a critical percentage of diploid cardiomyocytes is required to achieve heart regeneration. Our data identify cardiomyocyte polyploidization as a barrier to heart regeneration and suggest that mobilizing rare diploid cardiomyocytes in the human heart will improve its regenerative capacity.
Performance Testing and Analysis of Synchronous Reluctance Motor Utilizing Dual-Phase Magnetic Material

While interior permanent magnet (IPM) machines have been considered state of the art for traction motors, synchronous reluctance (SynRel) motors with advanced materials can provide a competitive alternative. IPM machines typically utilize neodymium iron boron permanent magnets, which pose an issue in terms of price, sustainability, demagnetization at higher operating temperatures, and uncontrolled generation. On the other hand, SynRel machines do not contain any magnets and are free from these issues. However, the absence of magnets as well as the presence of bridges and centerposts limit the flux-weakening capability of a SynRel machine, and limit the achievable constant power speed ratio without having to significantly oversize the machine and/or the power converter. In this paper, a new material referred to as the dual-phase magnetic material, where nonmagnetic regions can be selectively introduced within each lamination, will be evaluated for SynRel designs. The dual-phase feature of this material enables nonmagnetic bridges and posts, eliminating one of the key limitations of the SynRel designs in terms of torque density and flux weakening. This paper will present the design, analysis, and test results of an advanced proof-of-concept SynRel design utilizing dual-phase material with traction applications as the ultimate target application.
What are people looking for when they Google "self-abortion"?

Abstract Objective To examine the motivations and circumstances of individuals seeking information about self-abortion on the Internet. Study design We identified 26 terms that we anticipated someone might use to find information about self-abortion on the internet. Users who entered these terms into the Google search engine were provided with a link to our survey via Google AdWords. We fielded the survey over a 32-day period; users were eligible if accessing the survey from a US-based device. We examined demographic characteristics of the sample, reasons for searching for information on self-abortion, knowledge of the legality of abortion and of nearby providers, and top performing keywords. Results Our Google AdWords campaign containing the survey link was shown approximately 210,000 times, and clicked 9,800 times; 1,235 respondents completed the survey. The vast majority of the sample was female (96%), and 41% were minors. Almost three-quarters (73%) indicated that they were searching for information because they were pregnant and did not or may not want to be. Eleven percent had ever attempted to self-abort. One-third of respondents did not know if abortion was legal in their state of residence, and knowledge of legality did not differ by age. Conclusions There is interest in learning more about self-abortion on the Internet. Our findings suggest that, among those who participated in our survey, online searches for information on self-abortion may be driven by adolescents and young adults facing an unintended pregnancy. Implications Young women, in particular, may have an unmet need for information about safe and accessible abortion options.
Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa

When subjected to rapid drops of temperature (cold shock), Neurospora responds with a temporary shift in its morphology. This report is the first to examine this response genetically. We report here the results of a screen of selected mutants from the Neurospora knockout library for alterations in their morphological response to cold shock. Three groups of knockouts were selected to be subject to this screen: genes previously suspected to be involved in hyphal development as well as knockouts resulting in morphological changes; transcription factors; and genes homologous to E. coli genes known to alter their expression in response to cold shock. A total of 344 knockout strains were subjected to cold shock. Of those, 118 strains were identified with altered responses. We report here the cold shock morphologies and GO categorizations of strains subjected to this screen. Of strains with knockouts in genes associated with hyphal growth or morphology, 33 of 131 tested (25%) showed an altered response to cold shock. Of strains with knockouts in transcription factor genes, 30 of 145 (20%) showed an altered response to cold shock. Of strains with knockouts in genes homologous to E. coli genes which display altered levels of transcription in response to cold shock, a total of 55 of 68 tested (81%) showed an altered cold shock response. This suggests that the response to cold shock in these two organisms is largely shared in common.
The History, Policy Implications, and Knowledge Gaps of the CCTV Literature: Insights for the Development of Body-Worn Video Camera Research

Closed-circuit television (CCTV) and body-worn video cameras (BWVCs) have rapidly spread throughout policing. Such widespread deployment has heightened the importance of identifying best practices...
An Intrinsic Role of Beta Oscillations in Memory for Time Estimation

The neural mechanisms underlying time perception are of vital importance to a comprehensive understanding of behavior and cognition. Recent work has suggested a supramodal role for beta oscillations in measuring temporal intervals. However, the precise function of beta oscillations and whether their manipulation alters timing has yet to be determined. To accomplish this, we first re-analyzed two, separate EEG datasets and demonstrate that beta oscillations are associated with the retention and comparison of a memory standard for duration. We next conducted a study of 20 human participants using transcranial alternating current stimulation (tACS), over frontocentral cortex, at alpha and beta frequencies, during a visual temporal bisection task, finding that beta stimulation exclusively shifts the perception of time such that stimuli are reported as longer in duration. Finally, we decomposed trialwise choice data with a drift diffusion model of timing, revealing that the shift in timing is caused by a change in the starting point of accumulation, rather than the drift rate or threshold. Our results provide evidence for the intrinsic involvement of beta oscillations in the perception of time, and point to a specific role for beta oscillations in the encoding and retention of memory for temporal intervals.